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Abstract Open Science Partnerships (OSPs) are gaining attention as alternatives to university–industry collaborations with restrictive IPR and knowledge sharing policies. OSPs have different expected outcomes and deploy varying means to reach them. Appreciating these differences is crucial to understanding their scientific and socio-economic impact, and yet these differences have never been systematically investigated. This exploratory study draws on qualitative case studies of five biomedical OSPs involving academic partners and pharmaceutical companies. It identifies key elements—purpose, activities and structure—that can be used to describe how OSPs are designed. We identify two key aspects of purpose—predominant intent and research aims—which we argue affect the activities and structure of an OSP. Based on these two aspects, we propose four ideal types of OSPs that are designed to provide a starting point for researchers who explore the nature and impact of OSPs and for practitioners who are developing OSPs and wish to ensure that they deploy appropriate means to meet the intended outcomes of their partnership.

Summary: Background: Long-term SARS-CoV-2 adverse health outcomes are of significant concern, especially among young adults with the potential for the greatest long-term morbidity. We sought to assess and characterize these outcomes in a cohort of Marines. Methods: We used a cohort of US Marines from a previous longitudinal, prospective observational study of acute SARS-CoV-2, most of whom were enrolled prior to infection. A panel study was established to assess for post-acute sequelae of COVID-19 (PASC), defined as symptoms at least 4 weeks after symptom onset or diagnosis. Symptoms were assessed through questionnaires and validated quality of health metrics. Periodic US Marine Corps fitness testing metrics provided an additional standardized functional assessment and were compared to a pre-pandemic cohort. Findings: Globally dispersed Marine participants (n = 899) seen an average of 330 days following initial enrollment were predominately male (n = 825, 91.7%), White (n = 613, 71.6%) or Black (n = 149, 17.4%) with a median age of 18 years (interquartile range: 18–19). Among 798 SARS-CoV-2 infected participants, 197 (24.7%) developed PASC. The most prevalent symptoms were loss of taste and/or smell (n = 82; 41.6%), shortness of breath (n = 74; 37.6%), and cough (n = 45; 22.8%). Those with PASC had higher rates and severity of somatic (p

University reputation is a decisive factor in the management, positioning, sustainability, competitiveness, differentiation, and success of universities. However, the measurement of university performance is often communicated through international rankings, which are frequently criticized for not considering the student's perception, who is the primary user of the educational service. For this reason, the objective is to analyze how students' experiences and their perceived value of the educational process influence the final reputation of universities in two different contexts: Colombia and Spain. Additionally, the results of these relationships are contrasted with the cultural differences and educational systems of both countries. The methodology used is quantitative by means of the formulation and demonstration of a structural equation model, with 385 surveys collected from undergraduate students in Colombia and 333 from undergraduate students in Spain. The results confirmed that student experience influences both perceived value and reputation, and perceived value influences the reputation of the university, likewise, the student experience has an indirect effect on reputation through perceived value, with consistent findings in both countries.

University reputation is the result of the assessments that the stakeholders make of the university, especially the user of the education service, the student. The literature has shown an important impact of reputation on the student’s perception of the university, and it has been related to success, competitiveness, sustainability, stakeholder decision-making and the differential of the value offer. Therefore, its evaluation has been the subject of several studies that seek to measure and assess reputation in higher education to understand how to manage it. This article evaluates the psychometric properties of the reputation scale in students at private universities in Colombia. The methodology used included an adaptation of the scale to the Spanish language, content validity analysis, reliability analysis, exploratory factor analysis and confirmatory factor analysis. The results showed the validity and reliability of the scale and, through the analyses carried out, provided evidence of the scale’s robustness.

Whether the long-term treatment of patients with proton pump inhibitors (PPIs) with different diseases [GERD, Zollinger–Ellison syndrome (ZES), etc.] can result in vitamin B12 (VB12) deficiency is controversial. In this study, in 175 patients undergoing long-term ZES treatment with anti-acid therapies, drug-induced control acid secretory rates were correlated with the presence/absence of VB12 deficiency, determined by assessing serum VB12 levels, measurements of VB12 body stores (blood methylmalonic acid (MMA) and total homocysteine[tHYC]), and other features of ZES. After a mean of 10.2 yrs. of any acid treatment (5.6 yrs. with PPIs), 21% had VB12 deficiency with significantly lower serum and body VB12 levels (p < 0.0001). The presence of VB12 deficiency did not correlate with any feature of ZES but was associated with a 12-fold lower acid control rate, a 2-fold higher acid control pH (6.4 vs. 3.7), and acid control secretory rates below those required for the activation of pepsin (pH > 3.5). Over a 5-yr period, the patients with VB12 deficiency had a higher rate of achlorhydria (73% vs. 24%) and a lower rate of normal acid secretion (0% vs. 49%). In conclusion, in ZES patients, chronic long-term PPI treatment results in marked acid hyposecretion, resulting in decreased serum VB12 levels and decreased VB12-body stores, which can result in VB12 deficiency.

Abstract DNA methylation comprises a cumulative record of lifetime exposures superimposed on genetically determined markers. Little is known about methylation dynamics in humans following an acute perturbation, such as infection. We characterized the temporal trajectory of blood epigenetic remodeling in 133 participants in a prospective study of young adults before, during, and after asymptomatic and mildly symptomatic SARS‐CoV‐2 infection. The differential methylation caused by asymptomatic or mildly symptomatic infections was indistinguishable. While differential gene expression largely returned to baseline levels after the virus became undetectable, some differentially methylated sites persisted for months of follow‐up, with a pattern resembling autoimmune or inflammatory disease. We leveraged these responses to construct methylation‐based machine learning models that distinguished samples from pre‐, during‐, and postinfection time periods, and quantitatively predicted the time since infection. The clinical trajectory in the young adults and in a diverse cohort with more severe outcomes was predicted by the similarity of methylation before or early after SARS‐CoV‐2 infection to the model‐defined postinfection state. Unlike the phenomenon of trained immunity, the postacute SARS‐CoV‐2 epigenetic landscape we identify is antiprotective.

The sustainable design of product systems has been addressed in numerous research works. However, studies regarding the sustainability of services are fewer and they are focused mostly on the study of environmental aspects during the service provision stage. Regarding cultural services, the application of sustainability principles in their design process is increasingly required due to reasons such as environmental awareness, social responsibility, visitors' preferences and innovation strategies. Although up to date a limited number of initiatives have been promoted. The objectives of this work are the description of a specific sustainable design method based on the development of systematic tasks and supported on three complementary approaches, and the application of this method to the design of two different types of cultural exhibitions. The design of an itinerary exhibition, which is developed in a sheltered indoor space, and an exhibition, which is developed in the streets of a big city, are, respectively, carried out. The sustainability performance of different design alternatives are valued in each case by analyzing the incidence of those systems, activities, and stakeholders involved along the entire service development and using not only environmental but also socio-economic indicators. Thus, this research work aims at providing the appropriate methodological support to designers of cultural services and managers of cultural institutions, to carry out more sustainable projects.

Infections caused by acute respiratory viruses induce a systemic innate immune response, which can be measured by the increased levels of expression of inflammatory genes in immune cells. There is growing evidence that these acute viral infections, alongside transient transcriptomic responses, induce epigenetic remodeling as part of the immune response, such as DNA methylation and histone modifications, which might persist after the infection is cleared. In this article, we first review the primary mechanisms of epigenetic remodeling in the context of innate immunity and inflammation, which are crucial for the regulation of the immune response to viral infections. Next, we delve into the existing knowledge concerning the impact of respiratory virus infections on the epigenome, focusing on Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2), Influenza A Virus (IAV), and Respiratory Syncytial Virus (RSV). Finally, we offer perspectives on the potential consequences of virus-induced epigenetic remodeling and open questions in the field that are currently under investigation.

Introduction: The actin regulatory protein, cofilin plays a key signaling role in many cells for numerous cellular responses including in proliferation, development, motility, migration, secretion and growth. In the pancreas it is important in islet insulin secretion, growth of pancreatic cancer cells and in pancreatitis. However, there are no studies on its role or activation in pancreatic acinar cells.Methods: To address this question, we studied the ability of CCK to activate cofilin in pancreatic acinar cells, AR42J cells and CCK1-R transfected Panc-1 cells, the signaling cascades involved and its effect on enzyme secretion and MAPK activation, a key mediator of pancreatic growth.Results: CCK (0.3 and 100 nM), TPA, carbachol, Bombesin, secretin and VIP decreased phospho-cofilin (i.e., activate cofilin) and both phospho‐kinetic and inhibitor studies of cofilin, LIM kinase (LIMK) and Slingshot Protein Phosphatase (SSH1) demonstrated these conventional activators of cofilin were not involved. Serine phosphatases inhibitors (calyculin A and okadaic acid), however inhibited CCK/TPA-cofilin activation. Studies of various CCK‐activated signaling cascades showed activation of PKC/PKD, Src, PAK4, JNK, ROCK mediated cofilin activation, but not PI3K, p38, or MEK. Furthermore, using both siRNA and cofilin inhibitors, cofilin activation was shown to be essential for CCK-mediated enzyme secretion and MAPK activation.Conclusion: These results support the conclusion that cofilin activation plays a pivotal convergent role for various cell signaling cascades in CCK mediated growth/enzyme secretion in pancreatic acini.

Summary: Assays detecting blood transcriptome changes are studied for infectious disease diagnosis. Blood-based RNA alternative splicing (AS) events, which have not been well characterized in pathogen infection, have potential normalization and assay platform stability advantages over gene expression for diagnosis. Here, we present a computational framework for developing AS diagnostic biomarkers. Leveraging a large prospective cohort of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection and whole-blood RNA sequencing (RNA-seq) data, we identify a major functional AS program switch upon viral infection. Using an independent cohort, we demonstrate the improved accuracy of AS biomarkers for SARS-CoV-2 diagnosis compared with six reported transcriptome signatures. We then optimize a subset of AS-based biomarkers to develop microfluidic PCR diagnostic assays. This assay achieves nearly perfect test accuracy (61/62 = 98.4%) using a naive principal component classifier, significantly more accurate than a gene expression PCR assay in the same cohort. Therefore, our RNA splicing computational framework enables a promising avenue for host-response diagnosis of infection. Motivation: Host-based response assays (HRAs) can often diagnose infectious disease earlier and more precisely than pathogen-based tests. However, the role of RNA alternative splicing (AS) in HRAs remains unexplored, as existing HRAs are restricted to gene expression signatures. We report a computational framework for the identification, optimization, and evaluation of blood AS-based diagnostic assay development for infectious disease. Using SARS-CoV-2 infection as a case study, we demonstrate the improved accuracy of AS biomarkers for COVID-19 diagnosis when compared against six reported transcriptome signatures and when implemented as a microfluidic PCR diagnostic assay.

Abstract Synthesis research in ecology and environmental science improves understanding, advances theory, identifies research priorities, and supports management strategies by linking data, ideas, and tools. Accelerating environmental challenges increases the need to focus synthesis science on the most pressing questions. To leverage input from the broader research community, we convened a virtual workshop with participants from many countries and disciplines to examine how and where synthesis can address key questions and themes in ecology and environmental science in the coming decade. Seven priority research topics emerged: (1) diversity, equity, inclusion, and justice (DEIJ), (2) human and natural systems, (3) actionable and use‐inspired science, (4) scale, (5) generality, (6) complexity and resilience, and (7) predictability. Additionally, two issues regarding the general practice of synthesis emerged: the need for increased participant diversity and inclusive research practices; and increased and improved data flow, access, and skill‐building. These topics and practices provide a strategic vision for future synthesis in ecology and environmental science.

La historia de la publicidad nos ha mostrado cómo ésta evoluciona, muta, cambia y se adapta a los cambios sociales, económicos, políticos, culturales…siempre con un objetivo y fin, sorprender, atraer y seducir a los diferentes públicos objetivos de las marcas. Buscando además la eficacia y la rentabilidad. En los últimos años, Internet y el nuevo contexto digital han supuesto toda una revolución tecnológica generando nuevos escenarios para la comunicación publicitaria, modificando canales ya existentes, y alterando no solo contenidos y narrativas, sino también su producción y consumo. El paisaje de los medios de comunicación y la conceptualización estratégica de las campañas publicitarias se ha ido adaptando a esta nueva situación. La relación entre la publicidad y los avances tecnológicos del mundo digital tiene también un impacto social, ético y legal que debe ser tenido en cuenta por la industria publicitaria y estudiado en el ámbito académico. Respetar la información personal y la privacidad de los datos es una cuestión crítica. Hemos pasado de la comunicación unilateral dominada por los grandes medios y los gobiernos a una sociedad del conocimiento en la que los ciudadanos no solo pueden ser difusores de información, sino también co-crear nuevos contenidos de formas antes limitadas. Este contexto global, inmediato y participativo, tiene sus propias características de difusión y se ha visto en los últimos años como un espacio de expresión y difusión de bulos y fake news. Tener este caldo de cultivo y utilizar el mencionado anonimato como medio para la impunidad, promueve el odio y la intolerancia en línea. Es importante señalar que, en este marco online, la comunicación en Internet y las redes sociales está dotada de una serie de peculiaridades, de entre las que se hace especial hincapié en la experiencia del anonimato y la desinhibición que genera el sentimiento de virtualidad. Autores como López y Sánchez (2019) destacan la importancia de la convivencia digital al proponer actitudes frente al mundo virtual y digital. La actitud es un factor clave que se utiliza para interactuar con otros usuarios. Al desarrollar y reconocer esta actitud, podemos identificar riesgos que pueden vulnerar la intimidad y la privacidad en la forma en que interactuamos con cada dispositivo. Para autores como De Haro (2019), las redes sociales actualmente pueden formar otras redes independientes más generales que los aíslen de otros usuarios de Internet y creen espacios seguros para catalizar diferentes comportamientos humanos y brindar nuevas formas de comunicación y aprendizaje (Holcomb y Bill, 2010). Por otro lado, los cambios y desafíos de los últimos tiempos también nos muestran un mundo cada vez más preocupado por un consumo responsable, sostenible y ecológico. Al paradigma de la sostenibilidad, se une también el de la igualdad e inclusión, el consumidor envejece, y vemos como un target maduro creciente y con poder económico se incorpora a este contexto digital de forma gradual. Desde el 2020 la complicada situación geopolítica, con la sucesión de crisis y emergencias sanitaria, climática, política, bélica y energética, no da tregua, nos enfrentamos una nueva realidad económica y social, de carácter global, que plantea nuevos desafíos, a los ya existentes en el sector de la publicidad y las relaciones públicas. Ante este panorama, a las marcas no les basta con sorprender y llamar la atención de los consumidores, sino ganar su confianza y respeto. Por ello, invitamos a presentar manuscritos originales que desde el ámbito de la investigación científica contribuyan a profundizar en cómo se están diseñando y adaptando, desde las marcas y la industria publicitaria, las estrategias de comunicación para sorprender, innovar y ganar la confianza y el respeto según el público objetivo, target o segmentos poblacionales.

IntroductionIntegrating the heterogeneity of small-scale agriculture with the regulation, material, and non-material contributions is key to complementing the rural-support policy instruments. The objectives of the present study were to analyze the diversity of agricultural types of management in small-scale maize agroecosystems and discuss their implications for nature's contributions in the region of Valles Altos, México.MethodsThe methodology was conducted by constructing an agricultural management typology with multivariate statistical analysis for 112 small plots interviews. The operationalization of regulation, material, and non-material nature's contributions was based on the definition and counting of cultural elements from agronomic management for each class of contribution.ResultsThe results indicate three different types of agricultural management defined mainly by the type of seed, the destination of harvest, and the type of tillage. This management diversity is guided by farmers' motivation to achieve food self-sufficiency or generate income from grain sales. Each management type has a unique provision of regulation, material, and no material contributions defined by the use of the native seed, use of stover, and management diversification.DiscussionThe integration of farm typology methods and nature's contributions framework reveals that it is critical to establish new incentives that include the biological and cultural diversity of agroecosystems and the individual motivations of farmers. This may help conserve the natural and cultural values of agriculture and design appropriate incentives for small-scale agriculture.

ABSTRACT We investigated the temporal profile of multiple components of the serological response after asymptomatic or mildly symptomatic SARS-CoV-2 infection, in a cohort of 67 previously SARS-CoV-2 naive young adults, up to 8.5 months after infection. We found a significant decrease of spike IgG and neutralization antibody titers from early (11 to 56 days) to late (4 to 8.5 months) time points postinfection. Over the study period, S1-specific IgG levels declined significantly faster than that of the S2-specific IgG. Further, serum antibodies from PCR-confirmed participants cross-recognized S2, but not S1, of the betacoronaviruses HKU1 and OC43, suggesting a greater degree of cross-reactivity of S2 among betacoronaviruses. Antibody-Dependent Natural Killer cell Activation (ADNKA) was detected at the early time point but significantly decreased at the late time point. Induction of serum Antibody-Dependent Monocyte Phagocytosis (ADMP) was detected in all the infected participants, and its levels remained stable over time. Additionally, a reduced percentage of participants had detectable neutralizing activity against the Beta (50%), Gamma (61 to 67%), and Delta (90 to 94%) variants, both early and late postinfection, compared to the ancestral strain (100%). Antibody binding to S1 and RBD of Beta, Gamma, Delta (1.7 to 2.3-fold decrease), and Omicron (10 to 16-fold decrease) variants was also significantly reduced compared to the ancestral SARS-CoV-2 strain. Overall, we found variable temporal profiles of specific components and functionality of the serological response to SARS-CoV-2 in young adults, which is characterized by lasting, but decreased, neutralizing activity and antibody binding to S1, stable ADMP activity, and relatively stable S2-specific IgG levels. IMPORTANCE Adaptive immunity mediated by antibodies is important for controlling SARS-CoV-2 infection. While vaccines against COVID-19 are currently widely distributed, a high proportion of the global population is still unvaccinated. Therefore, understanding the dynamics and maintenance of the naive humoral immune response to SARS-CoV-2 is of great importance. In addition, long-term responses after asymptomatic infection are not well-characterized, given the challenges in identifying such cases. Here, we investigated the longitudinal humoral profile in a well-characterized cohort of young adults with documented asymptomatic or mildly symptomatic SARS-CoV-2 infection. By analyzing samples collected preinfection, early after infection and during late convalescence, we found that, while neutralizing activity decreased over time, high levels of serum S2 IgG and Antibody-Dependent Monocyte Phagocytosis (ADMP) activity were maintained up to 8.5 months after infection. This suggests that a subset of antibodies with specific functions could contribute to long-term protection against SARS-CoV-2 in convalescent unvaccinated individuals.

Summary: The ongoing evolution of SARS-CoV-2 requires monitoring the capability of immune responses to cross-recognize Variants of Concern (VOC). In this cross-sectional study, we examined serological and cell-mediated immune memory to SARS-CoV-2 variants, including Omicron, among a cohort of 18-21-year-old Marines with a history of either asymptomatic or mild SARS-CoV-2 infection 6 to 14 months earlier. Among the 210 participants in the study, 169 were unvaccinated while 41 received 2 doses of mRNA-based COVID-19 vaccines. Vaccination of previously infected participants strongly boosted neutralizing and binding activity and memory B and T cell responses including the recognition of Omicron, compared to infected but unvaccinated participants. Additionally, no measurable differences were observed in immune memory in healthy young adults with previous symptomatic or asymptomatic infections, for ancestral or variant strains. These results provide mechanistic immunological insights into population-based differences observed in immunity against Omicron and other variants among individuals with different clinical histories.

Introduction: Quarantining is commonly used to mitigate the spread of SARS-CoV-2. However, questions remain regarding what specific interventions are most effective. Methods: After a 2-week home quarantine, U.S. Marine Corps recruits underwent a supervised 2-week quarantine at a hotel from August 11 to September 21, 2020. All recruits were assessed for symptoms through oral questioning and had their temperatures checked daily. Study participants answered a written clinical questionnaire and were tested for SARS-CoV-2 by polymerase chain reaction shortly after arrival in quarantine and on Days 7 and 14. The results were compared with those of a previously reported Marine-supervised quarantine at a college campus from May until July 2020 utilizing the same study, laboratory, and statistical procedures. Results: A total of 1,401 of 1,514 eligible recruits (92.5%) enrolled in the study, 93.1% of whom were male. At the time of enrollment, 12 of 1,401 (0.9%) participants were polymerase chain reaction positive for SARS-CoV-2, 9 of 1,376 (0.7%) were positive on Day 7, and 1 of 1,358 (0.1%) was positive on Day 14. Only 12 of 22 (54.5%) participants endorsed any symptoms on a study questionnaire, and none of the participants had an elevated temperature or endorsed symptoms during daily screening for SARS-CoV-2. Participation rate (92%) was much greater than the approximately 58.8% (1,848 of 3,143) rate observed in the previous Marine-supervised college campus quarantine, suggesting the changing attitudes of recruits during the pandemic (p

The ErbB RTKs (EGFR, HER2, HER3, and HER4) have been well-studied in cancer. EGFR, HER2, and HER3 stimulate cancer proliferation, principally by activating the phosphatidylinositol-3-kinase and extracellular signal-regulated kinase (ERK) pathways, resulting in increased cancer cell survival and proliferation. Cancer cells have high densities of the EGFR, HER2, and HER3 causing phosphorylation of tyrosine amino acids on protein substrates and tyrosine amino acids near the C-terminal of the RTKs. After transforming growth factor (TGF) α binds to the EGFR, homodimers or EGFR heterodimers form. HER2 forms heterodimers with the EGFR, HER3, and HER4. The EGFR, HER2, and HER3 are overexpressed in lung cancer patient tumors, and monoclonal antibodies (mAbs), such as Herceptin against HER2, are used to treat breast cancer patients. Patients with EGFR mutations are treated with tyrosine kinase inhibitors, such as gefitinib or osimertinib. Peptide GPCRs, such as NTSR1, are present in many cancers, and neurotensin (NTS) stimulates the growth of cancer cells. Lung cancer proliferation is impaired by SR48692, an NTSR1 antagonist. SR48692 is synergistic with gefitinib at inhibiting lung cancer growth. Adding NTS to lung cancer cells increases the shedding of TGFα, which activates the EGFR, or neuregulin-1, which activates HER3. The transactivation process is impaired by SRC, matrix metalloprotease, and reactive oxygen species inhibitors. While the transactivation process is complicated, it is fast and occurs within minutes after adding NTS to cancer cells. This review emphasizes the use of tyrosine kinase inhibitors and SR48692 to impair transactivation and cancer growth.

In a study of US Marine recruits, seroprevalence of severe acute respiratory syndrome coronavirus 2 IgG was 9.0%. Hispanic and non-Hispanic Black participants and participants from states affected earlier in the pandemic had higher seropositivity rates. These results suggest the need for targeted public health strategies among young adults at increased risk for infection.

Young adults infected with SARS-CoV-2 are frequently asymptomatic or develop only mild disease. Because capturing representative mild and asymptomatic cases require active surveillance, they are less characterized than moderate or severe cases of COVID-19. However, a better understanding of SARS-CoV-2 asymptomatic infections might shed light into the immune mechanisms associated with the control of symptoms and protection. To this aim, we have determined the temporal dynamics of the humoral immune response, as well as the serum inflammatory profile, of mild and asymptomatic SARS-CoV-2 infections in a cohort of 172 initially seronegative prospectively studied United States Marine recruits, 149 of whom were subsequently found to be SARS-CoV-2 infected. The participants had blood samples taken, symptoms surveyed and PCR tests for SARS-CoV-2 performed periodically for up to 105 days. We found similar dynamics in the profiles of viral load and in the generation of specific antibody responses in asymptomatic and mild symptomatic participants. A proteomic analysis using an inflammatory panel including 92 analytes revealed a pattern of three temporal waves of inflammatory and immunoregulatory mediators, and a return to baseline for most of the inflammatory markers by 35 days post-infection. We found that 23 analytes were significantly higher in those participants that reported symptoms at the time of the first positive SARS-CoV-2 PCR compared with asymptomatic participants, including mostly chemokines and cytokines associated with inflammatory response or immune activation (i.e., TNF-α, TNF-β, CXCL10, IL-8). Notably, we detected 7 analytes (IL-17C, MMP-10, FGF-19, FGF-21, FGF-23, CXCL5 and CCL23) that were higher in asymptomatic participants than in participants with symptoms; these are known to be involved in tissue repair and may be related to the control of symptoms. Overall, we found a serum proteomic signature that differentiates asymptomatic and mild symptomatic infections in young adults, including potential targets for developing new therapies and prognostic tests.

The interplay between science and society takes place through a wide range of intertwined relationships and mutual influences that shape each other and facilitate continuous knowledge flows. Stylised consequentialist perspectives on valuable knowledge moving from public science to society in linear and recursive pathways, whilst informative, cannot fully capture the broad spectrum of value creation possibilities. As an alternative we experiment with an approach that gathers together diverse science-society interconnections and reciprocal research-related knowledge processes that can generate valorisation. Our approach to value creation attempts to incorporate multiple facets, directions and dynamics in which constellations of scientific and societal actors generate value from research. The paper develops a conceptual model based on a set of nine value components derived from four key research-related knowledge processes: production, translation, communication, and utilization. The paper conducts an exploratory empirical study to investigate whether a set of archetypes can be discerned among these components that structure science-society interconnections. We explore how such archetypes vary between major scientific fields. Each archetype is overlaid on a research topic map, with our results showing the distinctive topic areas that correspond to different archetypes. The paper finishes by discussing the significance and limitations of our results and the potential of both our model and our empirical approach for further research.

Smartphone use influences teenagers’ behaviors and lifestyles, not always in a positive way. Abuse and dependence on the use of this device is what has led to the study of nomophobia. The objective of this research is to measure the level of nomophobia in adolescents, and to study their digital consumption habits. The study seeks to analyze the relationships between risk of nomophobia, digital behavior, age and smartphone use. A structured questionnaire has been applied to a sample of 850 students aged 12 to 16. The data has been analyzed with SPSS and SPAD. Multivariate statistical characterization, one of the most recent data mining techniques, has been used to study differences in teenagers’ behaviors according to their risk of nomophobia, and to find related explanatory variables. Teenagers’ nomophobia risk ranges from mild to moderate, showing a relation with age, academic performance and intensity of use of mobile social networking apps. The risk of nomophobia responds to differences in students’ digital, social, relational and educational behaviors, and exhibits differences according to academic performance, age, gender, motivation and self-perception.

G-protein-coupled receptors (GPCRs) are increasingly being considered as possible therapeutic targets in cancers. Activation of GPCR on tumors can have prominent growth effects, and GPCRs are frequently over-/ectopically expressed on tumors and thus can be used for targeted therapy. CNS/neural tumors are receiving increasing attention using this approach. Gliomas are the most frequent primary malignant brain/CNS tumor with glioblastoma having a 10-year survival

We investigated serological responses following a SARS-CoV-2 outbreak in spring 2020 on a US Marine recruit training base. 147 participants that were isolated during an outbreak of respiratory illness were enrolled in this study, with visits approximately 6 and 10 weeks post-outbreak (PO). This cohort is comprised of young healthy adults, ages 18-26, with a high rate of asymptomatic infection or mild symptoms, and therefore differs from previously reported longitudinal studies on humoral responses to SARS-CoV-2, which often focus on more diverse age populations and worse clinical presentation. 80.9% (119/147) of the participants presented with circulating IgG antibodies against SARS-CoV-2 spike (S) receptor-binding domain (RBD) at 6 weeks PO, of whom 97.3% (111/114) remained positive, with significantly decreased levels, at 10 weeks PO. Neutralizing activity was detected in all sera from SARS-CoV-2 IgG positive participants tested (n=38) at 6 and 10 weeks PO, without significant loss between time points. IgG and IgA antibodies against SARS-CoV-2 RBD, S1, S2, and the nucleocapsid (N) protein, as well neutralization activity, were generally comparable between those participants that had asymptomatic infection or mild disease. A multiplex assay including S proteins from SARS-CoV-2 and related zoonotic and human endemic betacoronaviruses revealed a positive correlation for polyclonal cross-reactivity to S after SARS-CoV-2 infection. Overall, young adults that experienced asymptomatic or mild SARS-CoV-2 infection developed comparable humoral responses, with no decrease in neutralizing activity at least up to 10 weeks after infection.

Zika and dengue viruses are emergent arboviruses of great public health impact. Both viruses are responsible for important diseases, yet there is currently no vaccine or specific treatment available.

ABSTRACTHost switch events of influenza A viruses (IAVs) continuously pose a zoonotic threat to humans. In 2013, swine-origin H1N1 IAVs emerged in dogs soon after they were detected in swine in the Guangxi province of China. This host switch was followed by multiple reassortment events between these H1N1 and previously circulating H3N2 canine IAVs (IAVs-C) in dogs. To evaluate the phenotype of these newly identified viruses, we characterized three swine-origin H1N1 IAVs-C and one reassortant H1N1 IAV-C. We found that H1N1 IAVs-C predominantly bound to human-type receptors, efficiently transmitted via direct contact in guinea pigs and replicated in human lung cells. Moreover, the swine-origin H1N1 IAVs-C were lethal in mice and were transmissible by respiratory droplets in guinea pigs. Importantly, sporadic human infections with these viruses have been detected, and preexisting immunity in humans might not be sufficient to prevent infections with these new viruses. Our results show the potential of H1N1 IAVs-C to infect and transmit in humans, suggesting that these viruses should be closely monitored in the future.

Background: Gambling landscape has changed in recent years with the emergence of online gambling (OG). Greater accessibility and availability of this betting modality can increase the risk of developing a gambling disorder (GD). Online sports betting (OSB) is currently the most common type of OG, but little is known about the clinical characteristics of OSB compared to slot-machine (SM) gamblers, the most common offline gambling disorder.Methods: This was a prospective study conducted between October 2005 and September 2019, and included outpatients diagnosed with GD seen in a Pathological Gambling and Behavioral Addictions referral unit. Only patients with OSB and SM disorders were included. The main objective was to assess the clinical profile of OSB compared to SM gamblers, and to define clinical predictors for developing OSB gambling disorder. Logistic regression was performed to determine the effects of variables on the likelihood of this disorder.Results: Among 1,186 patients attended in our Unit during the study period, 873 patients were included; 32 (3.7%) were OSB gamblers and 841 (96.3%) were SM gamblers. Overall, mean age was 45 ± 13 years and 94.3% were men. Compared to SM patients, OSB patients were younger (34.9 ± 9.5 vs. 45.3 ±13), more frequently single (43.8 vs. 20.6%) and had a university education level (43.8 vs. 4.5%); they were also more frequently non-smokers (18.7 vs. 66.7%) and had fewer psychiatric comorbidities (12.5 vs. 29.4%) than SM gamblers. GD duration before treatment initiation was shorter in OSB patients than in SM gamblers, most of them (81.3 vs. 42.4%) with ≤ 5 years of GD duration. OSB gamblers showed significant differences in weekly gambling expenditure, spending higher amounts than SM patients. Younger age (OR: 0.919; 95% CI: 0.874–0.966), university education level (OR: 10.658; 95% CI: 3.330–34.119), weekly expenditure >100€ (OR: 5.811; 95% CI:1.544–21.869), and being a non-smoker (OR:13.248; 95% CI:4.332–40.517) were associated with an increased likelihood of OSB gambling behavior.Conclusions: We identified different profiles for OSB and SM gamblers. Younger age, university education level, higher weekly expenditure, and non-smoking habit were associated with OSB compared to SM disorders. Prevention strategies should help young people become aware of the severe risks of OSB.

Research funding is an important factor for public science. Funding may affect which research topics get addressed, and what research outputs are produced. However, funding has often been studied simplistically, using top-down or system-led perspectives. Such approaches often restrict analysis to confined national funding landscapes or single funding organizations and instruments in isolation. This overlooks interlinkages, broader funding researchers might access, and trends of growing funding complexity. This paper instead frames a 'bottom-up' approach that analytically distinguishes between increasing levels of aggregation of funding instrument co-use. Funding of research outputs is selected as one way to test this approach, with levels traced via funding acknowledgements (FAs) in papers published 2009-18 by researchers affiliated to Denmark, the Netherlands or Norway, in two test research fields (Food Science, Renewable Energy Research). Three funding aggregation levels are delineated: at the bottom, 'funding configurations' of funding instruments co-used by individual researchers (from single-authored papers with two or more FAs); a middle, 'funding amalgamations' level, of instruments co-used by collaborating researchers (from multi-authored papers with two or more FAs); and a 'co-funding network' of instruments co-used across all researchers active in a research field (all papers with two or more FAs). All three levels are found to include heterogenous funding co-use from inside and outside the test countries. There is also co-funding variety in terms of instrument 'type' (public, private, university or non-profit) and 'origin' (domestic, foreign or supranational). Limitations of the approach are noted, as well as its applicability for future analyses not using paper FAs to address finer details of research funding dynamics.

Resumen: Introducción: La actividad de guardia es un componente fundamental de la práctica médica y representa elevada carga asistencial y de tiempo laboral. Existen pocos estudios publicados que las analicen. Pacientes y métodos: Realizamos un estudio observacional, prospectivo, en el Hospital Virgen de la Salud de Toledo, hospital de tercer nivel. El objetivo principal fue describir la actividad asistencial de las guardias de medicina interna a partir de los motivos de consulta y llamadas al médico de guardia. Resultados: La media de pacientes ingresados en el hospital fue de 532, de los cuales 156 (29,4%) pacientes estaban ingresados en los servicios del equipo de guardia de medicina interna (medicina interna, oncología, neumología, endocrinología y reumatología son atendidos de guardia por medicina interna).Hubo una media de 30 consultas de enfermería y de especialista médico por guardia, una cada 18 minutos. Junto con los ingresos valorados en la guardia, suponen 43 actos asistenciales por guardia, uno cada 12 minutos.De las consultas realizadas por enfermería, aquellas por motivo burocrático representaron el 44% y por motivo clínico el 55%. Conclusión: Nuestro estudio refleja la importante actividad asistencial llevada a cabo durante las guardias de medicina interna.Un gran porcentaje de las consultas fueron de carácter burocrático, debiéndose implementar medidas que disminuyan las mismas.Un gran porcentaje fueron llamadas de carácter clínico en las que enfermería valoró presencialmente al paciente y precisó ayuda telefónica del médico.La facilidad de acceso y de comunicación médico-enfermería es imprescindible para asegurar una atención de calidad. Abstract: Introduction: On-call activity is a fundamental component of medical practice and represents a high burden of care and working time. There are few published studies that analyse these issues. Patients and methods: We conducted an observational, prospective study at the Virgen de la Salud Hospital in Toledo, a tertiary level hospital. The main objective was to describe the healthcare activity of internal medicine shifts based on the reasons for consultation and calls to the duty doctor. Results: The mean number of patients admitted to the hospital was 532, of which 156 (29.4%) were admitted to the departments of the duty internal medicine team (internal medicine, oncology, pneumology, endocrinology and rheumatology are attended by the duty internal medicine team).There was an average of 30 nursing and medical specialist consultations per shift, one every 18 minutes. Together with the admissions assessed during the shift, this represents 43 acts of care per shift, one every 12 minutes.Of the nurse consultations, 44% were for bureaucratic reasons and 55% for clinical reasons. Conclusion: Our study reflects the important healthcare activity carried out during internal medicine shifts.A large percentage of the consultations were bureaucratic in nature, and measures should be implemented to reduce them.A large percentage were clinical calls in which the nurse assessed the patient in person and required telephone help from the doctor.Ease of access and doctor-nurse communication is essential to ensure quality care.

ABSTRACT Irene Ramos works in the field of immunology to viral infections. In this mSphere of Influence article, she reflects on how “Global analyses of human immune variation reveal baseline predictors of postvaccination responses” by Tsang et al. (Cell 157:499–513, 2014, https://doi.org/10.1016/j.cell.2014.03.031) and “A crowdsourced analysis to identify ab initio molecular signatures predictive of susceptibility to viral infection” by Fourati et al. (Nat Commun 9:4418, 2018, https://doi.org/10.1038/s41467-018-06735-8) made an impact on her by highlighting the importance of data science methods to understand virus-host interactions.

The mandatory home confinement of the Spanish population, implemented in response to the COVID-19 pandemic, presents a unique opportunity to study the use and influence of Information and Communication Technologies (ICT) in people's perception of quality of life during this exceptional situation. This article adapts and validates a psychometric scale designed to identify and measure the main dimensions of the Quality of Life construct perceived through ICT use. To this end, an exploratory and transversal study has been carried out in Spain on a sample of 2,346 participants. Data processing has been carried out with SPSS and EQS. The results provide evidence of the reliability and psychometric quality on the scale, which exhibits adequate consistency that facilitates its application. The confirmatory factor analysis showed a hierarchical model of three correlated factors that account for the dimensions "Satisfaction with life", "Emotional support" and "Social support", which have enough correlation to measure the personal perception of quality of life associated with ICT use and are consistent with previous psychometric studies. The results of the TICO scale indicate that more than 70% of the sample feel ICT have united their family during home confinement and more than 45% experience happy feelings when they use ICT. In home confinement, ICT use has improved users' quality of life, mainly their satisfaction with life and social and family support.

We used epidemiologic and viral genetic information to identify a case of likely reinfection in an otherwise healthy, young Marine recruit enrolled in the prospective, longitudinal COVID-19 Health Action Response for Marines (CHARM) study, and we paired these findings with serological studies. This participant had a positive RT-PCR to SARS-CoV-2 upon routine sampling on study day 7, although he was asymptomatic at that time. He cleared the infection within seven days. On study day 46, he had developed symptoms consistent with COVID-19 and tested positive by RT-PCR for SARS-CoV-2 again. Viral whole genome sequencing was conducted from nares swabs at multiple time points. The day 7 sample was determined to be lineage B.1.340, whereas both the day 46 and day 49 samples were B.1.1. The first positive result for anti-SARS-CoV-2 IgM serology was collected on day 49 and for IgG on day 91. This case appears most consistent with a reinfection event. Our investigation into this case is unique in that we compared sequence data from more than just paired specimens, and we also assayed for immune response after both the initial infection and the later reinfection. These data demonstrate that individuals who have experienced an infection with SARS-CoV-2 may fail to generate effective or long-lasting immunity, similar to endemic human beta coronaviruses.

The differences in virus-host interactions resulting in distinct pathogenicity of seasonal and pandemic influenza A viruses (IAV) are not well understood. Here, the authors show that the hemagglutinin segment from pandemic, but not seasonal, IAV suppresses RIPK3-mediated dendritic cell death, thereby reducing T cell activation.

Vimentin is an intermediate filament protein that plays key roles in integration of cytoskeletal functions, and therefore in basic cellular processes such as cell division and migration. Consequently, vimentin has complex implications in pathophysiology. Vimentin is required for a proper immune response, but it can also act as an autoantigen in autoimmune diseases or as a damage signal. Although vimentin is a predominantly cytoplasmic protein, it can also appear at extracellular locations, either in a secreted form or at the surface of numerous cell types, often in relation to cell activation, inflammation, injury or senescence. Cell surface targeting of vimentin appears to associate with the occurrence of certain posttranslational modifications, such as phosphorylation and/or oxidative damage. At the cell surface, vimentin can act as a receptor for bacterial and viral pathogens. Indeed, vimentin has been shown to play important roles in virus attachment and entry of severe acute respiratory syndrome-related coronavirus (SARS-CoV), dengue and encephalitis viruses, among others. Moreover, the presence of vimentin in specific virus-targeted cells and its induction by proinflammatory cytokines and tissue damage contribute to its implication in viral infection. Here, we recapitulate some of the pathophysiological implications of vimentin, including the involvement of cell surface vimentin in interaction with pathogens, with a special focus on its role as a cellular receptor or co-receptor for viruses. In addition, we provide a perspective on approaches to target vimentin, including antibodies or chemical agents that could modulate these interactions to potentially interfere with viral pathogenesis, which could be useful when multi-target antiviral strategies are needed.

Neuropeptide G protein-coupled receptors (GPCRs) are overexpressed on numerous cancer cells. In a number of tumors, such as small cell lung cancer (SCLC), bombesin (BB) like peptides and neurotensin (NTS) function as autocrine growth factors whereby they are secreted from tumor cells, bind to cell surface receptors and stimulate growth. BB-drug conjugates and BB receptor antagonists inhibit the growth of a number of cancers. Vasoactive intestinal peptide (VIP) increases the secretion rate of BB-like peptide and NTS from SCLC leading to increased proliferation. In contrast, somatostatin (SST) inhibits the secretion of autocrine growth factors from neuroendocrine tumors (NETs) and decreases proliferation. SST analogs such as radiolabeled octreotide can be used to localize tumors, is therapeutic for certain cancer patients and has been approved for four different indications in the diagnosis/treatment of NETs. The review will focus on how BB, NTS, VIP, and SST receptors can facilitate the early detection and treatment of cancer.

Three cases of influenza A(H10N8) virus infection in humans have been reported; 2 of these infected persons died. Characterization of the receptor binding pattern of H10 hemagglutinin from avian and human isolates showed that both interact weakly with human-like receptors and maintain strong affinity for avian-like receptors.

While peptide antagonists for the gastrin-releasing peptide receptor (BB2R), neuromedin B receptor (BB1R), and bombesin (BB) receptor subtype-3 (BRS-3) exist, there is a need to develop non-peptide small molecule inhibitors for all three BBR. The BB agonist (BA)1 binds with high affinity to the BB1R, BB2R, and BRS-3. In this communication, small molecule BBR antagonists were evaluated using human lung cancer cells. AM-37 and ST-36 inhibited binding to human BB1R, BB2R, and BRS-3 with similar affinity (Ki = 1.4–10.8 µM). AM-13 and AM-14 were approximately an order of magnitude less potent than AM-37 and ST-36. The ability of BA1 to elevate cytosolic Ca2+ in human lung cancer cells transfected with BB1R, BB2R, and BRS-3 was antagonized by AM-37 and ST-36. BA1 increased tyrosine phosphorylation of the EGFR and ERK in lung cancer cells, which was blocked by AM-37 and ST-36. AM-37 and ST-36 reduced the growth of lung cancer cells that have BBR. The results indicate that AM-37 and ST-36 function as small molecule BB receptor antagonists.

Los eventos empresariales dentro de la estrategia de marketing relacional han sufrido, en los últimos años, una transformación sin precedentes tanto en su propia identidad como en los procesos propios de comunicación social que se desarrollan antes, durante y tras la celebración del evento. Junto con la estrategia de marketing tradicional y la vinculación con los stake-holders mediante estrategias relacionales, en el contexto actual de la sociedad digital, los social media se erigen como plataformas indispensables para interconectar e interactuar con los grupos de interés de las organizaciones empresariales a través de la comunicación 2.0 y las redes sociales. Este hecho amplía, sin duda, el potencial de las estrategias de comunicación y de relación que pueden aplicar las organizaciones empresariales en los acontecimientos corporativos y nos conduce a la necesidad de replantearnos una nueva forma de gestionar la marca-acontecimiento, así como un nuevo perfil profesional del gestor de eventos, vinculado a las funciones de figuras emergentes en el ámbito de la comunicación online, como son el Community Manager, el Content Manager o el Content Curator. A través de un muestreo intencional de doce acontecimientos corporativos de ámbito nacional e internacional desarrollados por empresas, asociaciones o entidades empresariales celebrados en nuestro país durante el año 2012, pretendemos analizar, mediante la metodología del case studies, si la planificación de las acciones implementadas en medios sociales ha sido configurada a partir de objetivos estratégicos definidos y, por tanto, está basada en una estrategia de comunicación 2.0, o bien, por el contrario, el uso de éstos representa una actividad puntual que empieza y acaba con el evento. Dicho análisis nos permitirá apuntar algunas directrices básicas que pueden servir para gestionar de forma profesionalizada la estrategia de marca en los eventos empresariales.

El artículo estudia el uso de Twitter como canal de comunicación corporativa y publicitaria. Para ello se realiza un análisis de contenido sobre los perfiles en este soporte de los principales anunciantes en España según el ranking de InfoAdex. Las variables definidas se centran en la descripción de los perfiles de los anunciantes, la frecuencia de publicación, el tipo de publicaciones y la repuesta e interacción que se obtiene de los usuarios, analizando la capacidad de los anunciantes de difundir contenidos y generar conversación, por una parte, y la reputación obtenida en esta red de microblogging, por otra.

The use of proton pump inhibitors (PPIs) over the last 30 years has rapidly increased both in the United States and worldwide. PPIs are not only very widely used both for approved indications (peptic ulcer disease, gastroesophageal reflux disease (GERD), Helicobacter pylori eradication regimens, stress ulcer prevention), but are also one of the most frequently off-label used drugs (25−70% of total). An increasing number of patients with moderate to advanced gastroesophageal reflux disease are remaining on PPI indefinitely. Whereas numerous studies show PPIs remain effective and safe, most of these studies are 10 years of treatment. Recently, based primarily on observational/epidemiological studies, there have been an increasing number of reports raising issues about safety and side-effects with very long-term chronic treatment. Some of these safety issues are related to the possible long-term effects of chronic hypergastrinemia, which occurs in all patients taking chronic PPIs, others are related to the hypo-/achlorhydria that frequently occurs with chronic PPI treatment, and in others the mechanisms are unclear. These issues have raised considerable controversy in large part because of lack of long-term PPI treatment data (>10−20 years). Zollinger−Ellison syndrome (ZES) is caused by ectopic secretion of gastrin from a neuroendocrine tumor resulting in severe acid hypersecretion requiring life-long antisecretory treatment with PPIs, which are the drugs of choice. Because in

Avian influenza virus infections in the human population are rare due to their inefficient direct human-to-human transmission. However, when humans are infected, a strong inflammatory response is usually induced, characterized by elevated levels of cytokines and chemokines in serum, believed to be important in the severe pathogenesis that develops in a high proportion of these patients. Extensive research has been performed to understand the molecular viral mechanisms involved in the H5N1 pathogenesis in humans, providing interesting insights about the virus-host interaction and the regulation of the innate immune response by these highly pathogenic viruses. In this review we summarize and discuss the most important findings in this field, focusing mainly on H5N1 virulence factors and their impact on the modulation of the innate immunity in humans.

El artículo realiza una revisión de tres perspectivas que intentan describir los cambios producidos en la segunda mitad del siglo XX en la investigación científica y tecnológica. Los tres enfoques analizados –“Modo 2”, “ciencia posacadémica” y “ciencia posnormal”– asocian estas transformaciones a una mayor permeabilidad de la ciencia hacia los intereses y preferencias de los principales actores sociales. Estos enfoques han tenido un gran impacto, no sólo en la literatura especializada, sino también en la retórica de los hacedores de las políticas de I+D en los últimos años. En este trabajo se aborda un análisis conjunto de sus aportaciones más destacables y sus principales rasgos con el fin de realizar un balance crítico.

Este estudio presenta un análisis comparativo de contenidos, usabilidad y accesibilidad de las sedes web de las nueve OTRI universitarias andaluzas a partir de la construcción de un esquema analítico para la evaluación de la calidad de las sedes web de las OTRI españolas. Se han realizado tres tipos de análisis complementarios mediante la creación, selección y aplicación de una serie de indicadores de contenido, usabilidad y accesibilidad respectivamente. Para el análisis de accesiblidad se han usado dos programas informáticos aplicados a 135 páginas, quince por cada sede web, con especial atención a sus páginas de acceso. La ponderación de las puntuaciones conseguidas en cada uno de los apartados se traslada a una nota final procedente de la media obtenida entre el test de contenidos, por un lado, y la puntuación media conjunta entre el test de usabilidad y accesibilidad por otro. La evaluación de las sedes web de las OTRI de las universidades andaluzas ha revelado la existencia de graves deficiencias que afectan a la calidad de las mismas, lo que produce un impacto negativo en la comunicación y difusión de sus funciones como institución de enlace en materia de I+D entre las universidades y la sociedad.

Este artículo recoge la creciente tendencia en favor de los datos abiertos generados por las administraciones públicas y aborda su reutilización mediante el uso de licencias-tipo. Tras exponer algunas propuestas internacionales de apertura de datos, se examina la incidencia en España a través del marco legislativo actual y algunas de las principales iniciativas existentes, que aún presentan determinadas carencias, entre las que destaca cierto déficit de interoperabilidad. A continuación se analiza la regulación normativa de la reutilización de datos mediante el uso de licencias-tipo y se reflexiona sobre su aplicación al caso español. Se constata que las licencias Creative Commons se adaptan a la legislación española vigente porque contemplan todas las modalidades de difusión y explotación recogidas en la misma, lo que hace su uso recomendable.

La comunicación y la publicidad viven actualmente inmersas en un proceso de cambio y evolución constantes, alimentados por la revolución social, cultural y tecnológica que ha supuesto la web 2.0. El trabajo que se presenta tiene como objetivo conocer las principales líneas de trabajo académico en el ámbito de las redes sociales y la web 2.0 en España, estudiando la proyección y el uso que se da a cada una de las metodologías de investigación científica. Para la realización del análisis se han seleccionado las publicaciones sobre este tema en las principales revistas de comunicación en España.

La coyuntura de crisis afecta al sector publicitario no solo en términos económicos. El auge de los medios no convencionales, el rol activo del destinatario, el aumento de la inversión en Internet y el afán de conectar emocionalmente con el público son otras de las características más relevantes de la comunicación publicitaria actual. En este contexto, nos encontramos con una nueva tendencia en el discurso publicitario: el interés por narrar historias que afectan directamente al ciudadano, apelando al compromiso con unos valores.

Las personas mayores se han convertido en un segmento de mercado muy interesante debido a su peso económico y demográfico, siendo sus miembros grandes consumidores de los medios de comunicación. En este sentido y desde ámbito de la investigación académica en comunicación, el público senior se presenta como un tema de investigación oportuno y actual. El objetivo general de este trabajo es conocer cómo se ha procedido en la investigación científica en los estudios sobre personas mayores y publicidad, realizando para ello un análisis de contenido de los artículos publicadas durante el periodo 19762012. Los resultados de este estudio muestran la metodología y los temas preponderantes por décadas e identifican, entre otros aspectos, las variables de estudio más analizadas.

El presente trabajo analiza la integración de los medios sociales en las estrategias de comunicación de las políticas sectoriales para personas mayores desarrolladas por los ayuntamientos españoles. A través de una aproximación a estas políticas como comunicación de proximidad de los entes locales, se analiza su presencia en la web y el uso de recursos 2.0 en los procesos comunicativorelacionales orientados a los ciudadanos mayores. Los resultados muestran la existencia de un amplio abanico de políticas dirigidas al público senior, cuyo grado de visibilidad y difusión a través de las webs sociales de las instituciones municipales es muy heterogéneo.

Esta investigación analiza la representación de los mayores en la publicidad televisiva española desde un enfoque de género. Se examina el contenido de 2065 spots de la franja horaria de máxima audiencia durante una semana en 2008 y de cinco cadenas españolas (TVE 1, La 2, Tele 5, Antena 3 y Cuatro). Los resultados revelan que las mujeres mayores están infrarrepresentadas en comparación con los datos demográficos. Igualmente los personajes femeninos y masculinos se muestran con características asociadas al estereotipo de género. Las conclusiones de este estudio están en consonancia con investigaciones previas de otros países. Entre las implicaciones destacan aquellas que atienden a la aplicación de un modelo más igualitario bajo la consideración de sus posibles efectos en el objetivo de la publicidad.

Myeloid blood cells are largely resistant to infection with human immunodeficiency virus type 1 (HIV-1). Recently, it was reported that Vpx from HIV-2/SIVsm facilitates infection of these cells by counteracting the host restriction factor SAMHD1. Here, we independently confirmed that Vpx interacts with SAMHD1 and targets it for ubiquitin-mediated degradation. We found that Vpx-mediated SAMHD1 degradation rendered primary monocytes highly susceptible to HIV-1 infection; Vpx with a T17A mutation, defective for SAMHD1 binding and degradation, did not show this activity. Several single nucleotide polymorphisms in the SAMHD1 gene have been associated with Aicardi-Goutières syndrome (AGS), a very rare and severe autoimmune disease. Primary peripheral blood mononuclear cells (PBMC) from AGS patients homozygous for a nonsense mutation in SAMHD1 (R164X) lacked endogenous SAMHD1 expression and support HIV-1 replication in the absence of exogenous activation. Our results indicate that within PBMC from AGS patients, CD14+ cells were the subpopulation susceptible to HIV-1 infection, whereas cells from healthy donors did not support infection. The monocytic lineage of the infected SAMHD1 -/- cells, in conjunction with mostly undetectable levels of cytokines, chemokines and type I interferon measured prior to infection, indicate that aberrant cellular activation is not the cause for the observed phenotype. Taken together, we propose that SAMHD1 protects primary CD14+ monocytes from HIV-1 infection confirming SAMHD1 as a potent lentiviral restriction factor.