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Chitosan has established itself as a multifunctional and auspicious biomaterial within the domain of tissue engineering, presenting a decade of uninterrupted advancements and novel implementations. This article provides a comprehensive overview of the most recent developments in chitosan-based tissue engineering, focusing on significant progress made in the last ten years. An exploration is conducted of the various techniques utilized in the modification of chitosan and the production of scaffolds, with an analysis of their effects on cellular reactions and tissue regeneration. The investigation focuses on the integration of chitosan with other biomaterials and the addition of bioactive agents to improve their functionalities. Upon careful analysis of the in vitro and in vivo research, it becomes evident that chitosan effectively stimulates cell adhesion, proliferation, and differentiation. Furthermore, we offer valuable perspectives on the dynamic realm of chitosan-based approaches tailored to distinct tissue categories, including nerve, bone, cartilage, and skin. The review concludes with a discussion of prospective developments, with particular attention given to possible directions for additional study, translational implementations, and the utilization of chitosan to tackle existing obstacles in the field of tissue engineering. This extensive examination provides a significant amalgamation of the advancements achieved over the previous decade and directs scholars towards uncharted territories in chitosan-based tissue engineering., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier B.V. All rights reserved.)

Background: The perennial evergreen tea (Camellia sinensis) plant is one of the most popular nonalcoholic drinks in the world. Fertilizers and industrial, agricultural, and municipal activities are the usual drivers of soil contamination, contaminating tea plants with potentially toxic elements (PTEs). These elements might potentially accumulate to larger amounts in the leaves of plants after being taken up from the soil. Thus, frequent monitoring of these elements is critically important., Methods: The present study intended to determine PTEs (Al, Cr, Mn, Fe, Co, Ni, Cu, Zn, Cd, and Pb) in both tea leaves and infusions using ICP-OES. Various multivariate data analysis methods such as principal component analysis (PCA) and hierarchical cluster analysis (HCA) were employed to elucidate the potential sources of PTEs contamination, whether from anthropogenic activities or natural origins. Additionally, Pearson's correlation coefficient (PCC) was calculated to assess the relationships between the variables under study., Results: The mean contents (mg/L) of all studied elements in tea infusions decreased in order Mn (150.59 ± 1.66) > Fe (11.39 ± 0.99) > Zn (6.62 ± 0.89) > Cu (5.86 ± 0.62) > Co (3.25 ± 0.64) > Ni (1.69 ± 0.23) > Pb (1.08 ± 0.16) > Cr (0.57 ± 0.09) > Cd (0.46 ± 0.09) > Al (0.05 ± 0.008), indicating that Mn exhibits the highest abundance. The mean concentration trend in tea leaf samples mirrored that of infusions, albeit with higher concentrations of PTEs in the former. The tolerable dietary intake (TDI) value for Ni and provisional tolerable monthly intake (PTMI) value for Cd surpassed the standards set by the WHO and EFSA. Calculated hazard index (HI < 1) and cumulative cancer risk (CCR) values suggest negligible exposure risk., Conclusion: Elevated levels of PTEs in commonly consumed tea products concern the public and regulatory agencies., Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier GmbH. All rights reserved.)

This study focuses on the investigation of the significance of polymers in drug delivery approaches. The carboxymethyl cellulose (CMC), polyvinyl alcohol (PVA) and dextrin-based hydrogel membrane were prepared and employed for the sustained release of third-generation oral antibiotic (cefixime). Different proportions of CMC, PVA and dextrin were blended and hydrogel membranes were fabricated via solvent casting method. The prepared membrane was characterized by FTIR, SEM, UV-visible, TGA and swelling analysis. Cefixime drug was incorporated in the CMC/PVA/dextrin matrix and drug release was investigated. The sustained release of the tested drug (cefixime) was investigated and the drug was released in 120 min in the phosphate-buffered saline (PBS) solution. The antibacterial activity of the prepared membrane was promising against Proteus vulgaris , salmonella typhi, Escherichia coli and Bacillus subtilis strains. The swelling capabilities, thermal stability and non-toxic nature of the prepared CMC/PVA/dextrin membrane could have potential applications for cefixime drug in delivery in a controlled way for the treatment of infectious diseases., Competing Interests: The author(s) declared no potential conflicts of interest with respect to the research, authorship, and/or publication of this article., (© The Author(s) 2024.)

A significant amount of plastic trash has been dumped into the environment across the world, contributing to the present white pollution crisis. Therefore, plastic manufacturing and disposal must be examined. Biodegradable plastics (BPs) have recently become the subject of study due to their beneficial biodegradability and harmlessness, and they have been the most efficient method for addressing the issue of plastic pollution. This study aims to enhance the synthesis of biodegradable polymers from sodium alginate (Na-Alg) with the addition of guar gum, corn starch, and gelatin using the solution-casting method, followed by mixing in suitable proportions and drying at a certain temperature, resulting in thin film formation. To enhance qualities of the already produced polymer, additional substances such as glycerol, PVA, and latex were added as plasticizers. Characterization techniques such as scanning electron microscopy (SEM), tensile strength, thermogravimetric analysis (TGA), energy dispersive spectroscopy (EDS), X-ray diffraction (XRD), differential scanning calorimetry (DSC), UV-vis spectroscopy, and Fourier transform infrared (FTIR) spectroscopy were used to study structural characteristics, surface morphology, polymeric linkages, water absorption capabilities, chemical conductivity, and light transmittance of the newly formed films. These characterization results depict a remarkable achievement in the sense of the high degradability and impressive tensile strength of the newly formed films. In addition, SEM images indicated a porous structure with interconnected pores. FT-IR confirms the occurrence of molecular interactions between separate components. Consequently, different films showed different behavior of degradability, and it is suggested from interpreting the results that the polymeric films may be a viable biodegradable option., Competing Interests: The authors declare no conflict of interest., (This journal is © The Royal Society of Chemistry.)

Ceftriaxone sodium belongs to the third-generation cephalosporin group and is used intramuscular and intravenous route as a broad-spectrum antibiotic. This research aims to prepare biocompatible hydrogels for targeted delivery of ceftriaxone sodium by parental route. Different proportions of polymers (natural and synthetic) in the presence of cross-linker were synthesized by solvent casting method. Ceftriaxone sodium was loaded in hydrogels in different concentrations and its drug release behavior was evaluated along with swelling and biodegradation analysis. The characterization of hydrogel was done by scanning electron microscopy (SEM) and Fourier transform infrared (FTIR) to analyze surface morphology and functional groups involved in the formation of dextrin/Na-alginate/PVA hydrogels loaded with the drug. Thermogravimetric analysis (TGA) was confirmed by thermal stability and degradation pattern of loaded and unloaded hydrogels. The drug-loaded samples presented promising antimicrobial activity against S. aureus and P. multocida and their cytotoxic nature was also studied. Drug release analysis using simulated intestinal fluid (SIF) and phosphate buffer saline(PBS) for the circulatory system shows the consistent release of the drug. The findings unveiled the development of a biocompatible and innovative hydrogel, which has potential advantages for biomedical application, particularly in enhancing the therapeutic efficacy of ceftriaxone sodium drug., Competing Interests: The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (© 2024 The Authors.)

Hydrogels are 3-dimensional polymer networks capable of absorbing a large amount of water. Natural polymeric hydrogels are biodegradable, non-toxic and biocompatible. They can effectively retain nutrients for the plant and can be used as soil conditioners. This study uses a chemical cross-linking technique to synthesize starch and chitosan-based hydrogel using citric acid as a cross-linker. Additionally, hydrogel composites were developed by incorporating wood ash, nano-cellulose, and NPK (nitrogen-phosphorus-potassium) fertilizer as fillers to enhance their properties. The formulated hydrogel/hydrogel composite samples were characterized by FTIR spectroscopy, SEM analysis, X-ray diffraction and thermo-gravimetric analysis. The experiment results showed the chemical cross-linking among the polymeric chain and the semi-crystalline nature of the hydrogel/hydrogel composite samples. The swelling capacity of the hydrogel/hydrogel composite samples was 200-420% (in distilled water) and 104-220% (in saline medium) and demonstrated biodegradability within 110 days. The NPK reinforced hydrogel composite showed an excellent effect on the growth of pea plants (leaves count = 37, stem height = 20.2 cm), and could be effectively used as soil conditioners for agricultural applications. Considering the ability of hydrogel composites to reduce irrigation needs, enhance nutrient retention, and improve crop production, these novel hydrogel composites present an economically viable solution for sustainable agricultural practices., Competing Interests: The authors declare no conflict of interest., (This journal is © The Royal Society of Chemistry.)

Orthopedic implants are an important tool in the treatment of musculoskeletal conditions and helped many patients to improve their quality of life. Various inorganic-organic biocomposites have been broadly investigated particularly in the area of load-bearing orthopedic/dental applications. Polyimide (PI) is a promising organic material and shows excellent mechanical properties, biocompatibility, bio-stability, and its elastic modulus is similar to human bone but it lacks bioactivity, which is very important for cell adhesion and ultimately for bone regeneration. In this research, tantalum pentoxide (Ta 2 O 5 ) coating was prepared on the surface of PI by polydopamine (PDA) bonding. The results showed that Ta 2 O 5 was evenly coated on the surface of PI, and with the concentration of Ta 2 O 5 in the PDA suspension increased, the content of Ta 2 O 5 particles on the surface of PI increased significantly. In addition, the Ta 2 O 5 coating significantly increased the roughness and hydrophilicity of the PI matrix. Cell experiments showed that PI surface coating Ta 2 O 5 could promote the proliferation, adhesion, and osteogenic differentiation of bone marrow-derived stromal cells (BMSCs). The results demonstrated that fabricating Ta 2 O 5 coating on the surface of PI through PDA bonding could improve the biocompatibility as well as bioactivity of PI, and increase the application potential of PI in the field of bone repair materials., Competing Interests: The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (© 2023 The Authors.)

The wound dressings fabricated by polymers and oregano essential oil (OEO) can be very effective as a hydrogel. The current study has been focused on fabricating the hydrogel membranes of oregano oil encapsulated as an antibacterial agent into sodium alginate (SA) solution by solvent casting method and then evaluated the antibacterial, antioxidant activity, and physicochemical performance of SA/OEO-based polymeric membranes. The polymeric interactions, surface morphology, water absorption capability, thermal stability, and encapsulation efficiency were investigated by FT-IR, SEM, swelling ratio, DSC, and encapsulation efficiency. The percentage encapsulation efficiency of essential oil was 40.5%. FTIR validated the presence of molecular interaction between individual components. SEM images showed a rough and porous appearance for hydrogel membranes. Moreover, DSC showed that the fabricated membranes were thermally stable. The inclusion of more content OEO decreased swelling ratios. The antioxidant test was carried out by DPPH assay and antibacterial test through disc diffusion method against microbes. The results revealed that membranes containing the highest content of OEO had more excellent antioxidant and antibacterial efficacy. Therefore, the polymeric membranes of sodium alginate loaded with oregano essential oil can be employed as an effective wound-healing candidate., Competing Interests: The author(s) declared no potential conflicts of interest with respect to the research, authorship, and/or publication of this article., (© The Author(s) 2023.)

Hydrogels are three-dimensional polymer networks that are hydrophilic and capable of retaining a large amount of water. Hydrogels also can act as vehicles for the controlled delivery of active compounds. Bio-polymers are polymers that are derived from natural sources. Hydrogels prepared from biopolymers are considered non-toxic, biocompatible, biodegradable, and cost-effective. Therefore, bio-polymeric hydrogels are being extensively synthesized and used all over the world. Hydrogels based on biopolymers finds important applications in the agricultural field where they are used as soil conditioning agents as they can increase the water retention ability of soil and can act as a carrier of nutrients and other agrochemicals. Hydrogels are also used for the controlled delivery of fertilizer to plants. In this review, bio-polymeric hydrogels based on starch, chitosan, guar gum, gelatin, lignin, and alginate polymer have been discussed in terms of their synthesis method, swelling behavior, and possible agricultural application. The urgency to address water scarcity and the need for sustainable water management in agriculture necessitate the exploration and implementation of innovative solutions. By understanding the synthesis techniques and factors influencing the swelling behavior of these hydrogels, we can unlock their full potential in fostering sustainable agriculture and mitigating the challenges posed by an ever-changing environment., Competing Interests: The authors declare no conflict of interest., (This journal is © The Royal Society of Chemistry.)

The generation of solid waste is increasing with each passing day due to rapid urbanization and industrialization and has become a matter of concern for the international community. Leachate leakages from landfills pollute the soil and can potentially harm the human health. In this paper, inductively coupled plasma-optical emission spectrometric studies were employed to assess and analyze the composition of metals (Ba, Cd, Pb, Hg, Cu, Cr and Mn) and metalloid (As) in soil samples. Results of Cr, Mn, Cu, As, Ba, Cd, Pb and Hg from CRM (certified reference material, SRM 2709a) of San Joaquin soil were evaluated and reported in terms of percent recoveries which were in the range of 97.6-102.9% and show outstanding extraction efficiency. Other than copper, where the permitted limit set by the EU is specified as 50-140 mg/kg in soil, the average amount of all the metals in soil was found within the permissible limits provided by WHO, the European Community (EU) and US EPA. Soil contaminated with Hg (PERI = 100) and Cd (PERI = 145.50) posed an ecological risk significantly. Pollution load index (PLI) value is greater than 1, while degree of contamination (C deg ) value is less than 32 which indicated that the soil is polluted and considerably contaminated with metals and metalloid, respectively. In terms of the average daily dosage (ADD) of soil, children received the highest doses of all metals (ADD ing  = 1.315 × 10 -7  - 2.470 × 10 -3 and ADD derm = 9.939 × 10 -7  - 5.292 × 10 -11 ), whereas ADD ing (1.409 × 10 -8  - 2.646 × 10 -4 ) was found greater in adults. For all metals except for Ba, the hazard quotient (HQ) trend in both children and adults was observed to be HQ ing  > HQ derm  > HQ inh of soil. Children who are at the lower edge of cancer risk had a lifetime cancer risk (LCR) of 2.039 × 10 -4 for Cr from various paths of soil exposure., (© 2023. The Author(s), under exclusive licence to Springer Nature B.V.)

Since ancient times, medicinal plants have been used as traditional medicine to treat a variety of ailments. Aloe vera (AV) gel's therapeutic potential is one of the most effective approach in the fabrication of functional materials. The current study aimed to prepare the AV and chitosan (CS) membranes using various cross-linkers that were characterized using X-ray diffraction (XRD), scanning electron microscopy (SEM), Fourier transform-infrared (FT-IR) spectroscopy, thermal gravimetric analysis (TGA), and ultraviolet-visible (UV-Visible) techniques, as well as swelling ratio and antimicrobial studies. SEM analysis revealed that the membrane is porous, with interconnected pores. The inclusion of AV contents in the membrane improved thermal stability and crystallinity. The swelling ratio of the ACPG-3 membrane with a 2:1 CS to AV ratio was 366%. The membranes showed promising antimicrobial activity against Escherichia coli, Staphylococcus aureus, Bacillus subtilis, and Pasteurella multocida strains. The findings revealed that polymeric CS/AV membranes have effective potential for use in the biomedical field., Competing Interests: The author(s) declared no potential conflicts of interest with respect to the research, authorship, and/or publication of this article., (© The Author(s) 2023.)

The production of synthetic drugs is considered a huge milestone in the healthcare sector, transforming the overall health, aging, and lifestyle of the general population. Due to the surge in production and consumption, pharmaceutical drugs have emerged as potential environmental pollutants that are toxic with low biodegradability. Traditional chromatographic techniques in practice are time-consuming and expensive, despite good precision. Alternatively, electroanalytical techniques are recently identified to be selective, rapid, sensitive, and easier for drug detection. Metal-organic frameworks (MOFs) are known for their intrinsic porous nature, high surface area, and diversity in structural design that provides credible drug-sensing capacities. Long-term reusability and maintaining chemo-structural integrity are major challenges that are countered by ligand-metal combinations, optimization of synthetic conditions, functionalization, and direct MOFs growth over the electrode surface. Moreover, chemical instability and lower conductivities limited the mass commercialization of MOF-based materials in the fields of biosensing, imaging, drug release, therapeutics, and clinical diagnostics. This review is dedicated to analyzing the various combinations of MOFs used for electrochemical detection of pharmaceutical drugs, comprising antibiotics, analgesics, anticancer, antituberculosis, and veterinary drugs. Furthermore, the relationship between the composition, morphology and structural properties of MOFs with their detection capabilities for each drug species is elucidated., Competing Interests: Competing InterestsThe authors declare no competing interests., (© The Author(s), under exclusive licence to Springer Nature Switzerland AG 2022, Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law.)

A series of chitosan/poly(vinyl alcohol)/guar gum (CS/PVA/GG) blends were prepared. The synthesis was carried out using different combinations of CS and GG, while keeping PVA constant by casting solution method. The effect of formaldehyde as a crosslinking agent was also evaluated. The blends were characterized by scanning electron microscopy (SEM), Fourier Transform Infra-red (FTIR) and X-ray powder diffraction (XRD). Additionally, the swelling ratio along with antimicrobial activity was also studied. SEM exhibited the phenomenon that surface morphology was mostly affected by blend ratios and cross-linker. The XRD shows the crystalline structure of blends. The FTIR confirmed the strong intermolecular bonding between polymers. Swelling exhibits that cross-linking affects the hydrophilicity of blends and swelling was excellent for S4 blend. The prepared blends showed promising antimicrobial activity against P. multocida, S. aureus, E. coli, and B. subtilis bacterial agents. The data concludes that GG, CS and PVA ternary blends could possibly be used for the biomedical applications., (Copyright © 2020 Elsevier B.V. All rights reserved.)

Biopolymers have paramount importance in pharmaceutical applications and blended biopolymers have shown promising properties versus individual counterparts. In view of excellent performance of blended biopolymers, a novel series of blended membranes based on chitosan, guar gum and polyvinyl alcohol (CS/GG/PVA) were prepared by solution casting technique. The membranes were synthesized at different ratios of GG and CS, while keeping PVA constant. The surface morphology, structure, interaction of polymers and water absorbing capacity were determined with scanning electron microscope (SEM), Fourier-transform infrared spectroscopy (FTIR), X-ray diffraction (XRD) and swelling properties. FTIR exhibited intermolecular bonding between the biopolymers used for membranes preparation. SEM analysis revealed that the surface morphology depends upon the various ratios of polymers. The swelling pattern has shown that the prepared membranes were hydrophilic in nature. Results revealed that the chitosan and guar gum based ternary blends with polyvinyl alcohol have potential for controlled drug release., (Copyright © 2019 Elsevier B.V. All rights reserved.)