1. The Processing and Regulation of Intronic miRNAs Are Independent of Their Host Genes in Arabidopsis.
- Author
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Li, Ying, Guo, Qianhuan, Wang, Meng, Zheng, Chengchao, and Yan, Kang
- Subjects
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MICRORNA , *ARABIDOPSIS , *GENES , *ARABIDOPSIS thaliana , *TRANSGENIC plants - Abstract
Background: MicroRNAs (miRNAs) have emerged as key regulators of post-transcriptional degradation and/or translational repression in both plants and animals. Increasing evidence has pointed to the important role of intergenic miRNAs in response to environmental stresses; however, detailed information about intronic miRNAs in plants is not clear. Results: Here, we performed quantitative real-time PCR (qRT-PCR) analysis using transgenic plants to investigate the relationship between intronic miRNAs and their respective host genes in Arabidopsis. Here, we found that three Arabidopsis thaliana intronic miRNAs (miR400, miR838, and miR848) were co-transcribed with their host genes in different organs. Intriguingly, both miR400 and its host gene (At1g32583) were up-regulated during cold stress. Unlike intronic miRNAs in animals, the change of expression levels of plant intronic miRNAs in transgenic lines did not affect the transcriptional levels of their host genes. This result indicates that there is no feedback regulation loop between the intronic miRNAs and their host genes in Arabidopsis. In miRNAs-cropping mutants, the mature miRNAs were significantly reduced, while the expression levels of host genes did not change, suggestting the microprocessor also play roles in intronic miRNA processing. Conclusions: Together, these results provide the evidence that there is an independent relationship between the processing of intronic miRNAs and their host genes in Arabidopsis. [ABSTRACT FROM AUTHOR] more...
- Published
- 2022
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