1. Alleviation of lipid metabolic dysfunction through regulation of intestinal bacteriophages and bacteria by green tea polyphenols in Ob/Ob mice.
- Author
-
Dong S, Wu S, Li L, Hao F, Wu J, Liao Z, Wang J, Zhong R, Wei H, and Fang X
- Subjects
- Animals, Mice, Male, Humans, Mice, Obese, Obesity metabolism, Obesity drug therapy, Obesity physiopathology, Obesity therapy, Obesity microbiology, Mice, Inbred C57BL, Intestines microbiology, Plant Extracts pharmacology, Plant Extracts administration & dosage, Diet, High-Fat adverse effects, Polyphenols pharmacology, Polyphenols administration & dosage, Polyphenols metabolism, Polyphenols chemistry, Gastrointestinal Microbiome drug effects, Bacteriophages metabolism, Tea chemistry, Bacteria genetics, Bacteria classification, Bacteria metabolism, Bacteria isolation & purification, Lipid Metabolism drug effects
- Abstract
Green tea polyphenols (GTP) have been shown to ameliorate lipid metabolic disorders by regulating intestinal bacteria. Given the significant role of intestinal bacteriophages in shaping the gut microbiota, this study investigates GTP's influence on gut bacteriophage-bacteria interactions and lipid metabolism using metagenomics and metabonomics. The research results indicated that GTP significantly reduced body weight, serum triglycerides, leptin, insulin resistance, interleukin-6, and TNF-α levels while increasing adiponectin in ob/ob mice fed high-fat diet, aiding intestinal repair. GTP improved gut health by decreasing Enterobacter, Siphoviridae and Enterobacteria_phage_sfv, increasing Bifidobacterium and intestinal metabolites SCFA and hippuric acid. Correlation analysis showed negative correlations between Enterobacter sp. 50,588,862 and Enterobacteria_phages, Shigella_phages with 4-hydroxyphenylpyruvate and hippuric acid. Bifidobacterium choerinum and Bifidobacterium sp. AGR2158 were positively correlated with fatty acids and bile acids. In conclusion, GTP reduced fat accumulation and inflammation, enhanced gut barrier function in obese mice, closely associated with changes in the gut bacteriophage community., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier Ltd. All rights reserved.)
- Published
- 2024
- Full Text
- View/download PDF