1. Leptin Receptor Gene Variant rs11804091 Is Associated with BMI and Insulin Resistance in Spanish Female Obese Children: A Case-Control Study
- Author
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Ramón Cañete, Angel Gil, Concepción M. Aguilera, Azahara I. Rupérez, Mercedes Gil-Campos, Rosaura Leis, Josune Olza, Rafael Tojo, [Olza, Josune] Univ Granada, Inst Nutr & Food Technol, Fac Pharm, Dept Biochem & Mol Biol 2, Ave Conocimiento S-N, Granada 18016, Spain, [Ruperez, Azahara I.] Univ Granada, Inst Nutr & Food Technol, Fac Pharm, Dept Biochem & Mol Biol 2, Ave Conocimiento S-N, Granada 18016, Spain, [Gil, Angel] Univ Granada, Inst Nutr & Food Technol, Fac Pharm, Dept Biochem & Mol Biol 2, Ave Conocimiento S-N, Granada 18016, Spain, [Aguilera, Concepcion M.] Univ Granada, Inst Nutr & Food Technol, Fac Pharm, Dept Biochem & Mol Biol 2, Ave Conocimiento S-N, Granada 18016, Spain, [Olza, Josune] Inst Salud Carlos III, CIBER Fisiopatol Obesidad & Nutr CIBEROBN, Madrid 28029, Spain, [Gil-Campos, Mercedes] Inst Salud Carlos III, CIBER Fisiopatol Obesidad & Nutr CIBEROBN, Madrid 28029, Spain, [Leis, Rosaura] Inst Salud Carlos III, CIBER Fisiopatol Obesidad & Nutr CIBEROBN, Madrid 28029, Spain, [Canete, Ramon] Inst Salud Carlos III, CIBER Fisiopatol Obesidad & Nutr CIBEROBN, Madrid 28029, Spain, [Gil, Angel] Inst Salud Carlos III, CIBER Fisiopatol Obesidad & Nutr CIBEROBN, Madrid 28029, Spain, [Aguilera, Concepcion M.] Inst Salud Carlos III, CIBER Fisiopatol Obesidad & Nutr CIBEROBN, Madrid 28029, Spain, [Olza, Josune] Inst Invest Biosanitaria Ibs GRANADA, Granada 18012, Spain, [Gil, Angel] Inst Invest Biosanitaria Ibs GRANADA, Granada 18012, Spain, [Aguilera, Concepcion M.] Inst Invest Biosanitaria Ibs GRANADA, Granada 18012, Spain, [Gil-Campos, Mercedes] Reina Sofia Univ Hosp, Maimonides Inst Biomed Res IMIBIC, Paediat Res & Metab Unit, Ave Menendez Pidal S-N, Cordoba 14010, Spain, [Canete, Ramon] Reina Sofia Univ Hosp, Maimonides Inst Biomed Res IMIBIC, Paediat Res & Metab Unit, Ave Menendez Pidal S-N, Cordoba 14010, Spain, [Leis, Rosaura] Univ Santiago de Compostela, Clin Univ Hosp Santiago, Dept Paediat, Unit Invest Nutr Growth & Human Dev Galicia, Travesia De Choupana 15706, Galicia, Spain, [Tojo, Rafael] Univ Santiago de Compostela, Clin Univ Hosp Santiago, Dept Paediat, Unit Invest Nutr Growth & Human Dev Galicia, Travesia De Choupana 15706, Galicia, Spain, Plan Nacional de Investigacion Cientifica, Desarrollo e Innovacion Tecnologica (I+D+I), Instituto de Salud Carlos III-Fondo de Investigacion Sanitaria, RETIC, Fondo Europeo De Desarrollo Regional (FEDER), and Universidade de Santiago de Compostela. Departamento de Ciencias Forenses, Anatomía Patolóxica, Xinecoloxía e Obstetricia, e Pediatría
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Male ,0301 basic medicine ,obesity ,medicine.medical_treatment ,Body Mass Index ,lcsh:Chemistry ,0302 clinical medicine ,insulin resistance ,Homeostasis ,genetic polymorphism ,Child ,lcsh:QH301-705.5 ,Spectroscopy ,LEPR gene ,child ,Neurons ,Linkage ,Leptin ,General Medicine ,Common ,Computer Science Applications ,Receptors, Leptin ,Female ,Risk ,Balance ,medicine.medical_specialty ,Adolescent ,Pomc ,030209 endocrinology & metabolism ,Biology ,Models, Biological ,Polymorphism, Single Nucleotide ,Article ,Catalysis ,Inorganic Chemistry ,03 medical and health sciences ,Sex Factors ,Insulin resistance ,Internal medicine ,medicine ,Humans ,Obesity ,Physical and Theoretical Chemistry ,Molecular Biology ,Genetic polymorphism ,Leptin receptor ,Adiponectin ,Insulin ,Organic Chemistry ,Quantitative insulin sensitivity check index ,medicine.disease ,Index ,Glucose ,030104 developmental biology ,Endocrinology ,lcsh:Biology (General) ,lcsh:QD1-999 ,Spain ,Case-Control Studies ,Body mass index ,Lepr polymorphisms - Abstract
Leptin is an endocrine hormone that has a critical role in body weight homoeostasis and mediates its effects via the leptin receptor (LEPR). Common polymorphisms in the genes coding leptin receptors have been associated with metabolic abnormalities. We assessed the association of 28 LEPR polymorphisms with body mass index (BMI) and their relationship with obesity-related phenotypes, inflammation and cardiovascular disease risk biomarkers. A multicentre case-control study was conducted in 522 children (286 with obesity and 236 with normal-BMI). All anthropometric, metabolic factors and biomarkers were higher in children with obesity except apolipoprotein (Apo)-AI, cholesterol, high-density lipoprotein cholesterol (HDL-c), and adiponectin, which were lower in the obesity group; and glucose, low-density lipoprotein cholesterol (LDL-c), and matrix metalloproteinase-9 that did not differ between groups. We identified the associations between rs11208659, rs11804091, rs10157275, rs9436303 and rs1627238, and BMI in the whole population, as well as the association of rs11804091, rs10157275, and rs1327118 with BMI in the female group, although only the rs11804091 remained associated after Bonferroni correction (p = 0.038). This single nucleotide polymorphisms (SNP) was also associated with insulin (p = 0.004), homeostasis model assessment for insulin resistance (HOMA-IR) (p = 0.006), quantitative insulin sensitivity check index (QUICKI) (p = 0.005) and adiponectin (p = 0.046) after adjusting for age, Tanner stage and BMI. Our results show a sex-specific association between the rs11804091 and obesity suggesting an influence of this SNP on insulin resistance., This work was supported by Plan Nacional de Investigación Científica, Desarrollo e Innovación Tecnológica (I+D+I), Instituto de Salud Carlos III-Fondo de Investigación Sanitaria (PI020826, PI051968, PI1102042, and PI1600871), RETIC (Red SAMID RD12/0026/0015) and Fondo Europeo De Desarrollo Regional (FEDER).
- Published
- 2017
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