Author: "Institut national de l'environnement industriel et des risques" - Searchworks@Jio Institute Digital Library Search Results

Your search keyword '"Institut national de l'environnement industriel et des risques"' showing total 5,142 results

Start Over Author "Institut national de l'environnement industriel et des risques"

5,142 results on '"Institut national de l'environnement industriel et des risques"'

1. TEXTO : Total EXposure To Organic Pollutants (TEXTO)

Author: Institut de Recherche en Santé, Environnement et le Travail, France, Laboratoire d'Etude des Résidus et Contaminants dans les Aliments, Institut national de l'environnement industriel et des risques, and National Agency for Sanitary Safety of the Food of the Environment and Labor
Published: 2015

2. Assessment of endocrine effects of xeno-estrogens and dioxine-like compounds on the expression of target genes and functional impacts on fish reproduction

Author: Brion, Francois, Pakdel, Farzad, Kah, Olivier, Monod, Gilles, Hinfray, Nathalie, Porcher, J.M., Station commune de recherches en ichtyophysiologie, biodiversité et environnement (SCRIBE), ., Institut National de l'Environnement Industriel et des Risques, ., Ministère de l'Ecologie et du Développement Durable, ., Centre National de la Recherche Scientifique, ., Institut National de l'Environnement Industriel et des Risques (INERIS), Université de Rennes (UR), Institut Fédératif de Recherche - Génétique Fonctionnelle Agronomie et Santé (IFR 140 GFAS), Station commune de Recherches en Ichtyophysiologie, Biodiversité et Environnement (SCRIBE), Institut National de la Recherche Agronomique (INRA), Ministère de l'Ecologie et du Développement Durable, Centre National de la Recherche Scientifique (CNRS), INRA, Université de Rennes 1 (UR1), and Université de Rennes (UNIV-RENNES)
Subjects: POISSON ZEBRE, ZEBRAFISH, TOXICOLOGIE, [SDV]Life Sciences [q-bio], MOSQUITOFISH, ESTROGENS, AROMATASE, VITELLOGENESIS, [INFO]Computer Science [cs], DIOXINS, SEX DIFFERENTIATION
Abstract: Programme national de recherche sur les perturbateurs endocriniens
Published: 2009

3. Guide d'échantillonnage de plantes potagères dans le cadre de diagnostics environnementaux

Author: Denys, S., Marot, F., Elreedy, S., Gobron, V., Dinant, S., Pinet, C., Dumat, C., Schwartz, Christophe, Jeannée, N., PROST, René, Pascal, M., Lemaitre, N., Leprieur, F., Mercat-Rommens, C., Tack, K., Cardenas, G., Douay, F., Barbaste, Mireille, Institut National de la Recherche Agronomique US 1118 Unité de Service Analyses Végétales et Environnementales, Institut National de la Recherche Agronomique UR 0251 Physico-chimie et Ecotoxicologie des Sols d'agrosystèmes contaminés, Institut National de la Recherche Agronomique UMR 1120 Sols et Environnement, Institut National de l'Environnement Industriel et des Risques, Agence de l'Environnement et de la Maîtrise de l'Energie, Agence Française de Sécurité Sanitaire de l'Environnement et du Travail, Agence Nationale pour la Gestion des Déchets Radioactifs, Conservatoire National des Arts et Métiers [CNAM], Ecole Nationale Supérieure Agronomique de Toulouse, Géovariances, Institut de Veille Sanitaire, Institut de Radioprotection et de Sureté Nucléaire, Institut Supérieur d'Agriculture de Lille (Groupe ISA), Institut National de l'Environnement Industriel et des Risques (INERIS), Agence de l'Environnement et de la Maîtrise de l'Energie (ADEME), Agence Française de Sécurité Sanitaire de l'Environnement et du Travail, Agence Nationale pour la Gestion des Déchets Radioactifs (ANDRA), Conservatoire National des Arts et Métiers [CNAM] (CNAM), École nationale supérieure agronomique de Toulouse [ENSAT], Laboratoire Sols et Environnement (LSE), Institut National de la Recherche Agronomique (INRA)-Université de Lorraine (UL), Geovariances, Unité de recherche Science du Sol (USS), Institut National de la Recherche Agronomique (INRA), Institut de Veille Sanitaire (INVS), Institut de Radioprotection et de Sûreté Nucléaire (IRSN), Institut Supérieur d'Agriculture de Lille (ISA), and Unité de service et de recherches en analyses végétales et environnementales (USRAVE)
Subjects: stratégie, [SDV]Life Sciences [q-bio], plante potagère, industrie, relation so-lplante-atmosphère, échantillonnage, état sanitaire, environnement, contamination, interprétation, [SDE]Environmental Sciences, pollution, ComputingMilieux_MISCELLANEOUS
Abstract: National audience
Published: 2007

4. Approches de la fiabilité dynamique pour modéliser des systèmes critiques

Author: Centre de recherche en automatique de Nancy (CRAN) ; CNRS - Université Henri Poincaré - Nancy I - Institut National Polytechnique de Lorraine (INPL), Institut Charles Delaunay (ICD) ; Université de Technologie de Troyes - CNRS, Université de Technologie de Troyes (UTT) ; Université de Technologie de Troyes, Institut Charles Delaunay Laboratoire de Modélisation et Sûreté des Systèmes (LM2S) ; Université de Technologie de Troyes, E.D.F. Division Recherche et Développement [Clamart] ; EDF, Institut National de l'Environnement Industriel et des Risques (INERIS) ; INERIS, Institut de Mathématiques de Bordeaux (IMB) ; Université Sciences et Technologies - Bordeaux I - Université Victor Segalen - Bordeaux II - CNRS, CQFD (INRIA Bordeaux - Sud-Ouest) ; CNRS - INRIA - Université de Bordeaux, Groupe de Recherche en Economie Théorique et Appliquée (GREThA) ; Université Montesquieu - Bordeaux IV - CNRS, Laboratoire Jean Kuntzmann (LJK) ; CNRS - Université Joseph Fourier - Grenoble I - Université Pierre-Mendès-France - Grenoble II - Institut Polytechnique de Grenoble - Grenoble Institute of Technology, Laboratoire d'Informatique, de Traitement de l'Information et des Systèmes (LITIS) ; Université du Havre - Université de Rouen - Institut National des Sciences Appliquées [INSA] - Rouen, Babykina, Génia, Barros, Anne, Bérenguer, Christophe, Bouissou, Marc, Brinzei, Nicolae, Brissaud, Florent, Broy, Perrine, Deleuze, Gilles, De Saporta, Benoîte, Dufour, François, Dijoux, Yann, Donat, Roland, Do Van, Phuc, Langeron, Yves, Nguyen, Danh Ngoc, Medjaher, Slimane, Zhang, Huilong, Centre de recherche en automatique de Nancy (CRAN) ; CNRS - Université Henri Poincaré - Nancy I - Institut National Polytechnique de Lorraine (INPL), Institut Charles Delaunay (ICD) ; Université de Technologie de Troyes - CNRS, Université de Technologie de Troyes (UTT) ; Université de Technologie de Troyes, Institut Charles Delaunay Laboratoire de Modélisation et Sûreté des Systèmes (LM2S) ; Université de Technologie de Troyes, E.D.F. Division Recherche et Développement [Clamart] ; EDF, Institut National de l'Environnement Industriel et des Risques (INERIS) ; INERIS, Institut de Mathématiques de Bordeaux (IMB) ; Université Sciences et Technologies - Bordeaux I - Université Victor Segalen - Bordeaux II - CNRS, CQFD (INRIA Bordeaux - Sud-Ouest) ; CNRS - INRIA - Université de Bordeaux, Groupe de Recherche en Economie Théorique et Appliquée (GREThA) ; Université Montesquieu - Bordeaux IV - CNRS, Laboratoire Jean Kuntzmann (LJK) ; CNRS - Université Joseph Fourier - Grenoble I - Université Pierre-Mendès-France - Grenoble II - Institut Polytechnique de Grenoble - Grenoble Institute of Technology, Laboratoire d'Informatique, de Traitement de l'Information et des Systèmes (LITIS) ; Université du Havre - Université de Rouen - Institut National des Sciences Appliquées [INSA] - Rouen, Babykina, Génia, Barros, Anne, Bérenguer, Christophe, Bouissou, Marc, Brinzei, Nicolae, Brissaud, Florent, Broy, Perrine, Deleuze, Gilles, De Saporta, Benoîte, Dufour, François, Dijoux, Yann, Donat, Roland, Do Van, Phuc, Langeron, Yves, Nguyen, Danh Ngoc, Medjaher, Slimane, and Zhang, Huilong
Abstract: National audience, Le projet consiste à expérimenter et comparer des approches de la fiabilité dynamique. L'enjeu est de modéliser de façon probabiliste la sûreté de fonctionnement des systèmes critiques, en particulier les interactions entre les processus physiques (modélisé par des variables continues) et le contrôle-commande (modélisé par des variables discrètes), en présence de défaillances (stochastiques) ou de contextes rares. Les domaines industriels visés sont la production d'énergie et les industries des procédés. Les partenaires sont l'Université de Technologie de Troyes (UTT-ICD), Nancy-Université-INPL (CRAN), Institut de Mathématiques de Bordeaux-INRIA (CQFD), EDF R&D. Le projet part du constat que les méthodes classiques, binaires ou par états, d'évaluation de la sûreté de fonctionnement (Arbres de Défaillance, Arbres d'Evènements, réseaux de Petri stochastiques) présentent des limites pour les systèmes critiques à caractère hybride.. Face à ces systèmes, qui combinent en général des variables continues de nature déterministe, et des évènements discrets de nature stochastique, les approches statiques et même dynamiques à espace d'états discret, peuvent conduire, passé un certain niveau de complexité ou de réalisme, à des simplifications excessives qui mettent en cause la crédibilité de l'analyse ou peuvent mener à des marges excessives, ou pire, à des parades inappropriées.

5. Main conclusions and perspectives from the collective scientific assessment of the effects of plant protection products on biodiversity and ecosystem services along the land–sea continuum in France and French overseas territories

Author: Stéphane Pesce, Laure Mamy, Wilfried Sanchez, Marcel Amichot, Joan Artigas, Stéphanie Aviron, Carole Barthélémy, Rémy Beaudouin, Carole Bedos, Annette Bérard, Philippe Berny, Cédric Bertrand, Colette Bertrand, Stéphane Betoulle, Eve Bureau-Point, Sandrine Charles, Arnaud Chaumot, Bruno Chauvel, Michael Coeurdassier, Marie-France Corio-Costet, Marie-Agnès Coutellec, Olivier Crouzet, Isabelle Doussan, Juliette Faburé, Clémentine Fritsch, Nicola Gallai, Patrice Gonzalez, Véronique Gouy, Mickael Hedde, Alexandra Langlais, Fabrice Le Bellec, Christophe Leboulanger, Christelle Margoum, Fabrice Martin-Laurent, Rémi Mongruel, Soizic Morin, Christian Mougin, Dominique Munaron, Sylvie Nélieu, Céline Pelosi, Magali Rault, Sergi Sabater, Sabine Stachowski-Haberkorn, Elliott Sucré, Marielle Thomas, Julien Tournebize, Sophie Leenhardt, Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Institut National de l'Environnement Industriel et des Risques (INERIS), Aix Marseille Université (AMU), Ecologie fonctionnelle et écotoxicologie des agroécosystèmes (ECOSYS), AgroParisTech-Université Paris-Saclay-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Université de Reims Champagne-Ardenne (URCA), Centre Norbert Elias (CNELIAS), École des hautes études en sciences sociales (EHESS)-Avignon Université (AU)-Aix Marseille Université (AMU)-Centre National de la Recherche Scientifique (CNRS), Centre National de la Recherche Scientifique (CNRS), Modélisation et écotoxicologie prédictives, Département biostatistiques et modélisation pour la santé et l'environnement [LBBE], Laboratoire de Biométrie et Biologie Evolutive - UMR 5558 (LBBE), Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut National de Recherche en Informatique et en Automatique (Inria)-VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-Centre National de la Recherche Scientifique (CNRS)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut National de Recherche en Informatique et en Automatique (Inria)-VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-Centre National de la Recherche Scientifique (CNRS)-Laboratoire de Biométrie et Biologie Evolutive - UMR 5558 (LBBE), Université de Lyon-Université de Lyon-Institut National de Recherche en Informatique et en Automatique (Inria)-VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-Centre National de la Recherche Scientifique (CNRS), Laboratoire Chrono-environnement (UMR 6249) (LCE), Centre National de la Recherche Scientifique (CNRS)-Université de Franche-Comté (UFC), Université Bourgogne Franche-Comté [COMUE] (UBFC)-Université Bourgogne Franche-Comté [COMUE] (UBFC), MARine Biodiversity Exploitation and Conservation (UMR MARBEC), Institut de Recherche pour le Développement (IRD)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Centre National de la Recherche Scientifique (CNRS)-Université de Montpellier (UM), RiverLy - Fonctionnement des hydrosystèmes (RiverLy), Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER), Institut Sophia Agrobiotech (ISA), Université Nice Sophia Antipolis (1965 - 2019) (UNS), COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Université Côte d'Azur (UCA), Laboratoire Microorganismes : Génome et Environnement (LMGE), Centre National de la Recherche Scientifique (CNRS)-Université Clermont Auvergne (UCA), Biodiversité agroécologie et aménagement du paysage (UMR BAGAP), Ecole supérieure d'Agricultures d'Angers (ESA)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Institut Agro Rennes Angers, Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro), Laboratoire Population-Environnement-Développement (LPED), Institut de Recherche pour le Développement (IRD)-Aix Marseille Université (AMU), Environnement Méditerranéen et Modélisation des Agro-Hydrosystèmes (EMMAH), Avignon Université (AU)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Interactions Cellules Environnement - UR (ICE), VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS), Centre de recherches insulaires et observatoire de l'environnement (CRIOBE), Université de Perpignan Via Domitia (UPVD)-École Pratique des Hautes Études (EPHE), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Centre National de la Recherche Scientifique (CNRS), Stress Environnementaux et BIOsurveillance des milieux aquatiques (SEBIO), Institut National de l'Environnement Industriel et des Risques (INERIS)-Université de Reims Champagne-Ardenne (URCA)-Université Le Havre Normandie (ULH), Normandie Université (NU)-Normandie Université (NU)-SFR Condorcet, Université de Reims Champagne-Ardenne (URCA)-Centre National de la Recherche Scientifique (CNRS)-Université de Reims Champagne-Ardenne (URCA)-Centre National de la Recherche Scientifique (CNRS), Agroécologie [Dijon], Université de Bourgogne (UB)-Université Bourgogne Franche-Comté [COMUE] (UBFC)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Institut Agro Dijon, Santé et agroécologie du vignoble (UMR SAVE), Université de Bordeaux (UB)-Institut des Sciences de la Vigne et du Vin (ISVV)-Ecole Nationale Supérieure des Sciences Agronomiques de Bordeaux-Aquitaine (Bordeaux Sciences Agro)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Dynamique et durabilité des écosystèmes : de la source à l’océan (DECOD), Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Office français de la biodiversité (OFB), Groupe de Recherche en Droit, Economie et Gestion (GREDEG), COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-Centre National de la Recherche Scientifique (CNRS)-Université Côte d'Azur (UCA), Laboratoire d'Etude et de Recherche sur l'Economie, les Politiques et les Systèmes Sociaux (LEREPS), Université Toulouse Capitole (UT Capitole), Université de Toulouse (UT)-Université de Toulouse (UT)-Université Toulouse - Jean Jaurès (UT2J), Université de Toulouse (UT)-Institut d'Études Politiques [IEP] - Toulouse-École Nationale Supérieure de Formation de l'Enseignement Agricole de Toulouse-Auzeville (ENSFEA), Environnements et Paléoenvironnements OCéaniques (EPOC), Observatoire aquitain des sciences de l'univers (OASU), Université Sciences et Technologies - Bordeaux 1 (UB)-Institut national des sciences de l'Univers (INSU - CNRS)-Centre National de la Recherche Scientifique (CNRS)-Université Sciences et Technologies - Bordeaux 1 (UB)-Institut national des sciences de l'Univers (INSU - CNRS)-Centre National de la Recherche Scientifique (CNRS)-École Pratique des Hautes Études (EPHE), Ecologie fonctionnelle et biogéochimie des sols et des agro-écosystèmes (UMR Eco&Sols), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Institut de Recherche pour le Développement (IRD)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Institut Agro Montpellier, Institut de l'Ouest : Droit et Europe (IODE), Université de Rennes (UR)-Centre National de la Recherche Scientifique (CNRS), Fonctionnement agroécologique et performances des systèmes de cultures horticoles (UPR HORTSYS), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad), Aménagement des Usages des Ressources et des Espaces marins et littoraux - Centre de droit et d'économie de la mer (AMURE), Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université de Brest (UBO)-Institut Universitaire Européen de la Mer (IUEM), Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS), Ecosystèmes aquatiques et changements globaux (UR EABX), Institut méditerranéen de biodiversité et d'écologie marine et continentale (IMBE), Avignon Université (AU)-Aix Marseille Université (AMU)-Institut de recherche pour le développement [IRD] : UMR237-Centre National de la Recherche Scientifique (CNRS), Universitat de Girona (UdG), Physiologie et Toxines des Microalgues Toxiques et Nuisibles (PHYTOX), Centre Universitaire de Formation et de Recherche de Mayotte (CUFR), Unité de Recherches Animal et Fonctionnalités des Produits Animaux (URAFPA), Université de Lorraine (UL)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Hydrosystèmes continentaux anthropisés : ressources, risques, restauration (UR HYCAR), Direction de l'Expertise scientifique collective, de la Prospective et des Etudes, and French Office for Biodiversity (OFB) through the national ECOPHYTO plan
Subjects: Health, Toxicology and Mutagenesis, [SDE.MCG]Environmental Sciences/Global Changes, Biocontrol, General Medicine, Expertise, Environment, Ecotoxicology, Pollution, Modelling, [SDE]Environmental Sciences, Environmental Chemistry, Pesticides, [SDE.BE]Environmental Sciences/Biodiversity and Ecology, Ecological risk assessment
Abstract: International audience; Preservation of biodiversity and ecosystem services is critical for sustainable development and human well-being. However, an unprecedented erosion of biodiversity is observed and the use of plant protection products (PPP) has been identified as one of its main causes. In this context, at the request of the French Ministries responsible for the Environment, for Agriculture and for Research, a panel of 46 scientific experts ran a nearly 2-year-long (2020-2022) collective scientific assessment (CSA) of international scientific knowledge relating to the impacts of PPP on biodiversity and ecosystem services. The scope of this CSA covered the terrestrial, atmospheric, freshwater, and marine environments (with the exception of groundwater) in their continuity from the site of PPP application to the ocean, in France and French overseas territories, based on international knowledge produced on or transposable to this type of context (climate, PPP used, biodiversity present, etc.). Here, we provide a brief summary of the CSA's main conclusions, which were drawn from about 4500 international publications. Our analysis finds that PPP contaminate all environmental matrices, including biota, and cause direct and indirect ecotoxicological effects that unequivocally contribute to the decline of certain biological groups and alter certain ecosystem functions and services. Levers for action to limit PPP-driven pollution and effects on environmental compartments include local measures from plot to landscape scales and regulatory improvements. However, there are still significant gaps in knowledge regarding environmental contamination by PPPs and its effect on biodiversity and ecosystem functions and services. Perspectives and research needs are proposed to address these gaps.
Published: 2023

6. ReadEDTest: A tool to assess the readiness of in vitro test methods under development for identifying endocrine disruptors

Author: Thibault Crouzet, Elise Grignard, François Brion, Etienne B. Blanc, Normand Podechard, Sophie Langouet, Paloma Alonso-Magdalena, Philippe Hubert, Min Ji Kim, Karine Audouze, Toxicité environnementale, cibles thérapeutiques, signalisation cellulaire (T3S - UMR_S 1124), Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Université Paris Cité (UPCité), PEPPER, Institut National de l'Environnement Industriel et des Risques (INERIS), Stress Environnementaux et BIOsurveillance des milieux aquatiques (SEBIO), Institut National de l'Environnement Industriel et des Risques (INERIS)-Université de Reims Champagne-Ardenne (URCA)-Université Le Havre Normandie (ULH), Normandie Université (NU)-Normandie Université (NU)-SFR Condorcet, Université de Reims Champagne-Ardenne (URCA)-Centre National de la Recherche Scientifique (CNRS)-Université de Reims Champagne-Ardenne (URCA)-Centre National de la Recherche Scientifique (CNRS), Institut de recherche en santé, environnement et travail (Irset), Université d'Angers (UA)-Université de Rennes (UR)-École des Hautes Études en Santé Publique [EHESP] (EHESP)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique ), École des Hautes Études en Santé Publique [EHESP] (EHESP), and Universidad Miguel Hernández [Elche] (UMH)
Subjects: OBERON, Pepper, [SDV]Life Sciences [q-bio], OECD, Validation process, IATA, Endocrine disrupting chemicals, Zebrafish embryo, General Environmental Science
Abstract: International audience; Growing evidence shows that endocrine disruptors (EDs), known to affect the reproductive system, may also disturb other hormone-regulated functions leading to cancers, neurodevelopmental defects, metabolic and immune diseases. To reduce exposure to EDs and limit their health effects, development of screening and mechanism-based assays to identify EDs is encouraged. Nevertheless, the crucial validation step of test methods by regulatory bodies is a time- and resource-consuming process. One of the main raisons of this long duration process is that method developers, mainly researchers, are not fully aware of the regulatory needs to validate a test. We propose an online self-assessment questionnaire (SAQ) called ReadEDTest easy to be used by all researchers. The aim of ReadEDTest is to speed up the validation process by assessing readiness criteria of in vitro and fish embryo ED test methods under development. The SAQ is divided into 7 sections and 13 sub-sections containing essential information requested by the validating bodies. The readiness of the tests can be assessed by specific score limits for each sub-section. Results are displayed via a graphical representation to help identification of the sub-sections having sufficient or insufficient information. The relevance of the proposed innovative tool was supported using two test methods already validated by the OECD and four under development test methods.
Published: 2023

7. An end-to-end pipeline based on open source deep learning tools for reliable analysis of complex 3D images of ovaries

Author: Manon Lesage, Manon Thomas, Thierry Pécot, Tu-Ky Ly, Nathalie Hinfray, Remy Beaudouin, Michelle Neumann, Robin Lovell-Badge, Jérôme Bugeon, Violette Thermes, Laboratoire de Physiologie et Génomique des Poissons (LPGP), Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique )-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique ), Institut National de l'Environnement Industriel et des Risques (INERIS), Stress Environnementaux et BIOsurveillance des milieux aquatiques (SEBIO), Institut National de l'Environnement Industriel et des Risques (INERIS)-Université de Reims Champagne-Ardenne (URCA)-Université Le Havre Normandie (ULH), Normandie Université (NU)-Normandie Université (NU)-SFR Condorcet, Université de Reims Champagne-Ardenne (URCA)-Centre National de la Recherche Scientifique (CNRS)-Université de Reims Champagne-Ardenne (URCA)-Centre National de la Recherche Scientifique (CNRS), and The Francis Crick Institute [London]
Subjects: Deep learning segmentation, Fish, 3D imaging, [SDV]Life Sciences [q-bio], Ovary, Clearing, Cellpose, Molecular Biology, Developmental Biology
Abstract: Computational analysis of bio-images by deep learning (DL) algorithms has made exceptional progress in recent years and has become much more accessible to non-specialists with the development of ready-to-use tools. The study of oogenesis mechanisms and female reproductive success has also recently benefited from the development of efficient protocols for three-dimensional (3D) imaging of ovaries. Such datasets have a great potential for generating new quantitative data but are, however, complex to analyze due to the lack of efficient workflows for 3D image analysis. Here, we have integrated two existing open-source DL tools, Noise2Void and Cellpose, into an analysis pipeline dedicated to 3D follicular content analysis, which is available on Fiji. Our pipeline was developed on larvae and adult medaka ovaries but was also successfully applied to different types of ovaries (trout, zebrafish and mouse). Image enhancement, Cellpose segmentation and post-processing of labels enabled automatic and accurate quantification of these 3D images, which exhibited irregular fluorescent staining, low autofluorescence signal or heterogeneous follicles sizes. In the future, this pipeline will be useful for extensive cellular phenotyping in fish or mammals for developmental or toxicology studies.
Published: 2023

8. Combinatorial pathway disruption is a powerful approach to delineate metabolic impacts of endocrine disruptors

Author: Kévin Bernal, Charbel Touma, Chedi Erradhouani, Talía Boronat‐Belda, Lucas Gaillard, Sara Al Kassir, Hélène Le Mentec, Corinne Martin‐Chouly, Normand Podechard, Dominique Lagadic‐Gossmann, Sophie Langouet, François Brion, Anja Knoll‐Gellida, Patrick J. Babin, Iva Sovadinova, Pavel Babica, Karine Andreau, Robert Barouki, Jan Vondracek, Paloma Alonso‐Magdalena, Etienne Blanc, Min Ji Kim, Xavier Coumoul, Toxicité environnementale, cibles thérapeutiques, signalisation cellulaire (T3S - UMR_S 1124), Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Université Paris Cité (UPCité), Institut de recherche en santé, environnement et travail (Irset), Université d'Angers (UA)-Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-École des Hautes Études en Santé Publique [EHESP] (EHESP)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique ), Institut National de l'Environnement Industriel et des Risques - INERIS (FRANCE), Centro de Investigación Biomédica en Red de Diabetes y Enfermedades Metabólicas Asociadas (CIBERDEM), Instituto de Salud Carlos III [Madrid] (ISC), Institut d'Investigació Biomèdica de Bellvitge [Barcelone] (IDIBELL), Laboratoire Maladies Rares: Génétique et Métabolisme (Bordeaux) (U1211 INSERM/MRGM), Université de Bordeaux (UB)-Groupe hospitalier Pellegrin-Institut National de la Santé et de la Recherche Médicale (INSERM), Univ Rennes, Inserm, EHESP, Irset, UMR S1085, Masaryk University [Brno] (MUNI), Institute of Biophysics of the Czech Academy of Sciences (IBP / CAS), Czech Academy of Sciences [Prague] (CAS), Université d'Angers (UA)-Université de Rennes (UR)-École des Hautes Études en Santé Publique [EHESP] (EHESP)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique ), Institut National de l'Environnement Industriel et des Risques (INERIS), The authors would like to thank the OBERON project (https://oberon-4eu.com/), funded by the European Union's Horizon 2020 research and innovation programme under grant agreement no. 825712, INSERM, Université Paris Cité and INERIS. IS and PB thank the RECETOX Research Infrastructure (no. LM2018121) and the CETOCOEN EXCELLENCE project (no. CZ.02.1.01/0.0/0.0/17_043/0009632) financed by the Ministry of Education, Youth and Sports for supportive background. This work was supported from the European Union's Horizon 2020 research and innovation programme under grant agreement no. 857560., European Project: 825712,H2020-EU.3.1.1. - Understanding health, wellbeing and disease,H2020-SC1-2018-Single-Stage-RTD,OBERON(2019), Jonchère, Laurent, and An integrative strategy of testing systems for identification of EDs related to metabolic disorders - OBERON - - H2020-EU.3.1.1. - Understanding health, wellbeing and disease2019-01-01 - 2019-12-31 - 825712 - VALID
Subjects: phthalate, perfluorinated compounds, [SDV]Life Sciences [q-bio], Biophysics, Cell Biology, dioxin, Biochemistry, appetite, bisphenol, inflammation, insulin resistance, microbiota, TBT, [SDV] Life Sciences [q-bio], Structural Biology, [SDV.TOX]Life Sciences [q-bio]/Toxicology, Genetics, Molecular Biology
Abstract: International audience; The prevalence of metabolic diseases, such as obesity, diabetes, metabolic syndrome and chronic liver diseases among others, has been rising for several years. Epidemiology and mechanistic (in vivo, in vitro and in silico) toxicology have recently provided compelling evidence implicating the chemical environment in the pathogenesis of these diseases. In this review, we will describe the biological processes that contribute to the development of metabolic diseases targeted by metabolic disruptors, and will propose an integrated pathophysiological vision of their effects on several organs. With regard to these pathomechanisms, we will discuss the needs, and the stakes of evolving the testing and assessment of endocrine disruptors to improve the prevention and management of metabolic diseases that have become a global epidemic since the end of last century.
Published: 2022

9. Ustekinumab is more effective than azathioprine to prevent endoscopic postoperative recurrence in Crohn's disease

Author: Buisson, Anthony, Nancey, Stéphane, Manlay, Luc, Rubin, David, Hebuterne, Xavier, Pariente, Benjamin, Fumery, Mathurin, Laharie, David, Roblin, Xavier, Bommelaer, Gilles, Pereira, Bruno, Peyrin‐biroulet, Laurent, Vuitton, Lucine, Post-Op Study Grp, Ustek, Service d'Hépatologie Gastro-entérologie [CHU Clermont-Ferrand], CHU Estaing [Clermont-Ferrand], CHU Clermont-Ferrand-CHU Clermont-Ferrand, Microbes, Intestin, Inflammation et Susceptibilité de l'Hôte (M2iSH), Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre de Recherche en Nutrition Humaine d'Auvergne (CRNH d'Auvergne)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Université Clermont Auvergne (UCA), Service d'Hépatogastroentérologie [CHU Lyon-Sud], Hospices Civils de Lyon (HCL), The University of Chicago Medicine [Chicago], Servcie Hépato-gastroentérolgie et Nutrition clinique [CHU Nice], Centre Hospitalier Universitaire de Nice (CHU Nice), Service Gastro-entérologie adulte – Maladies de l’appareil digestif [CHU Lille], CHU Lille, Service d'Hépato Gastroenterologie [CHU Amiens-Picardie], CHU Amiens-Picardie, Périnatalité et Risques Toxiques - UMR INERIS_I 1 (PERITOX), Institut National de l'Environnement Industriel et des Risques (INERIS)-Université de Picardie Jules Verne (UPJV)-CHU Amiens-Picardie, Service Gastroentérologie [CHU Bordeaux], Centre Hospitalier Universitaire de Bordeaux (CHU Bordeaux), Service de Gastro-entérologie et Hépatologie [CHU Saint-Etienne], Centre Hospitalier Universitaire de Saint-Etienne [CHU Saint-Etienne] (CHU ST-E), Unité de Biostatistiques [CHU Clermont-Ferrand], Direction de la recherche clinique et de l’innovation [CHU Clermont-Ferrand] (DRCI), Service d'Hépato-gastro-entérologie [CHRU Nancy], Centre Hospitalier Régional Universitaire de Nancy (CHRU Nancy), Service de Gastro-Entérologie [CHRU Besançon], Centre Hospitalier Régional Universitaire de Besançon (CHRU Besançon), 'Francois Aupetit' Association (AFA CrohnRCH), CHU Clermont-Ferrand (DRCI), Centre Hospitalier Universitaire de Lille (CHU Lille), Université de Picardie Jules Verne (UPJV)-CHU Amiens-Picardie-Institut National de l'Environnement Industriel et des Risques, Service de gastroentérologie [CHU Saint-Etienne], and Centre Hospitalier Universitaire de Saint-Etienne (CHU de Saint-Etienne)
Subjects: Adult, Male, medicine.medical_specialty, medicine.medical_treatment, Azathioprine, Antibodies, Monoclonal, Humanized, Gastroenterology, ustekinumab, law.invention, 03 medical and health sciences, 0302 clinical medicine, Crohn Disease, Gastrointestinal Agents, Randomized controlled trial, Recurrence, law, Internal medicine, Ustekinumab, Secondary Prevention, medicine, Clinical endpoint, Humans, postoperative recurrence, Propensity Score, Retrospective Studies, Crohn's disease, azathioprine, business.industry, Inflammatory Bowel Disease, [SDV.MHEP.HEG]Life Sciences [q-bio]/Human health and pathology/Hépatology and Gastroenterology, Bowel resection, medicine.disease, Control Groups, 3. Good health, Oncology, 030220 oncology & carcinogenesis, Cohort, Propensity score matching, Original Article, Female, 030211 gastroenterology & hepatology, business, Immunosuppressive Agents, medicine.drug
Abstract: International audience; Background: Preventing postoperative recurrence (POR) is a major concern in Crohn's disease (CD). While azathioprine is an option, no data is available on ustekinumab efficacy in this situation.Aims: We compared the effectiveness of ustekinumab versus azathioprine in preventing endoscopic POR in CD.Methods: We retrospectively collected data from all consecutive CD patients treated with ustekinumab after intestinal resection in 9 centers. The control group (azathioprine alone) was composed of patients who participated in a randomized controlled trial conducted in the same centers comparing azathioprine alone or in combination with curcumin. Propensity score analyses (inversed probability of treatment weighting = IPTW) were applied to compare the two groups. The primary endpoint was endoscopic POR (Rutgeerts' index ≥ i2) at 6 months.Results: Overall, 32 patients were included in the ustekinumab group and 31 in the azathioprine group. The propensity score analysis was adjusted on the main risk factors (smoking, fistulizing phenotype, prior bowel resection, resection length >30 cm and ≥2 biologics before surgery) and thiopurines or ustekinumab exposure prior to surgery making the two arms comparable (∣d∣ < 0.2). After IPTW, the rate of endoscopic POR at 6 months was lower in patients treated with ustekinumab compared to azathioprine (28.0% vs. 54.5%, p = 0.029). After IPTW, the rates of i2b-endoscopic POR (Rutgeerts' index ≥ i2b) and severe endoscopic POR (Rutgeerts' index ≥ i3) were 20.8% versus 42.5% (p = 0.066) and 16.9% versus 27.9% (p = 0.24), in the ustekinumab and azathioprine groups, respectively.Conclusion: Ustekinumab seemed to be more effective than azathioprine in preventing POR in this cohort of CD patients.
Published: 2021

10. Development of an exposure protocol for toxicity test (FEET) for a marine species: the European sea bass (Dicentrarchus labrax)

Author: Christelle Jozet-Alves, Sofia Soloperto, Christophe Minier, Salima Aroua, Marie-Pierre Halm-Lemeille, Stress Environnementaux et BIOsurveillance des milieux aquatiques (SEBIO), Université Le Havre Normandie (ULH), Normandie Université (NU)-Normandie Université (NU)-Université de Reims Champagne-Ardenne (URCA)-SFR Condorcet, Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS)-Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS)-Institut National de l'Environnement Industriel et des Risques (INERIS), Ethologie animale et humaine (EthoS), Université de Caen Normandie (UNICAEN), Normandie Université (NU)-Normandie Université (NU)-Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-Centre National de la Recherche Scientifique (CNRS), FR 3473 Institut universitaire Mer et Littoral (IUML), Le Mans Université (UM)-Université d'Angers (UA)-Université de Nantes (UN)-École Centrale de Nantes (ECN)-Université de Bretagne Sud (UBS)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Centre National de la Recherche Scientifique (CNRS), Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER), Normandie Region Region Normandie, urban community Le Havre Seine Metropole, IFREMER institute (program PENELOP), Research Federation FR CNRS [3730], European Union European Commission, urban community LHSM (Le Havre Seine Metropole), Institut National de l'Environnement Industriel et des Risques (INERIS)-Université de Reims Champagne-Ardenne (URCA)-Université Le Havre Normandie (ULH), Normandie Université (NU)-Normandie Université (NU)-SFR Condorcet, Université de Reims Champagne-Ardenne (URCA)-Centre National de la Recherche Scientifique (CNRS)-Université de Reims Champagne-Ardenne (URCA)-Centre National de la Recherche Scientifique (CNRS), and Normandie Université (NU)-Normandie Université (NU)-Université de Rennes (UR)-Centre National de la Recherche Scientifique (CNRS)
Subjects: 0106 biological sciences, 4-Dichloroaniline, Health, Toxicology and Mutagenesis, Behavioral test, Zoology, Temperate conditions, Context (language use), 010501 environmental sciences, Biology, Test (biology), 01 natural sciences, Marine species, Lethal Dose 50, 96 h LC50, Early-life stage toxicity test, Toxicity Tests, Environmental Chemistry, Ecotoxicology, Animals, 14. Life underwater, Sea bass, 0105 earth and related environmental sciences, Hatching, 010604 marine biology & hydrobiology, General Medicine, biology.organism_classification, Pollution, 3 4-Dichloroaniline, Larva, Toxicity, Sea bass larvae, Dicentrarchus, Bass, [SDV.TOX.ECO]Life Sciences [q-bio]/Toxicology/Ecotoxicology
Abstract: International audience; Regulatory assessment of the effects of chemicals requires the availability of validated tests representing different environments and organisms. In this context, developing new tests is particularly needed for marine species from temperate environments. It is also important to evaluate effects that are generally poorly characterized and seldom included in regulatory tests. In this study, we designed an exposure protocol using European sea bass (Dicentrarchus labrax) larvae. We examined classical toxicological values (LCx) as well as behavioral responses. By comparing different hatching and breeding strategies, we defined the optimal conditions of exposure as non-agitated conditions in 24- or 48-well microplates. Our exposure protocol was then tested with 3,4-dichloroaniline (3,4-DCA), a recommended reference molecule. Based on our results, the 96 h LC50 for 3,4-DCA corresponded to 2.04 mg/L while the 168 h LC50 to 0.79 mg/L. Behavioral analyses showed no effect of 3,4-DCA at low concentration (0.25 mg/L). In conclusion, the present work established the basis for a new test which includes behavioral analysis and shows that the use of sea bass is suitable to early-life stage toxicity tests.
Published: 2022

11. Interest of a multispecies approach in active biomonitoring: Application in the Meuse watershed

Author: Audrey Catteau, Jean-Marc Porcher, Anne Bado-Nilles, Isabelle Bonnard, Marc Bonnard, Arnaud Chaumot, Elise David, Odile Dedourge-Geffard, Laurence Delahaut, Nicolas Delorme, Adeline François, Laura Garnero, Christelle Lopes, Katherine Nott, Patrice Noury, Olivier Palluel, Mélissa Palos-Ladeiro, Hervé Quéau, Sébastien Ronkart, Khadija Sossey-Alaoui, Cyril Turiès, Bernard Tychon, Olivier Geffard, Alain Geffard, Stress Environnementaux et BIOsurveillance des milieux aquatiques (SEBIO), Institut National de l'Environnement Industriel et des Risques (INERIS)-Université de Reims Champagne-Ardenne (URCA)-Université Le Havre Normandie (ULH), Normandie Université (NU)-Normandie Université (NU)-SFR Condorcet, Université de Reims Champagne-Ardenne (URCA)-Centre National de la Recherche Scientifique (CNRS)-Université de Reims Champagne-Ardenne (URCA)-Centre National de la Recherche Scientifique (CNRS), Institut National de l'Environnement Industriel et des Risques (INERIS), Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Laboratoire de Biométrie et Biologie Evolutive - UMR 5558 (LBBE), Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut National de Recherche en Informatique et en Automatique (Inria)-VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-Centre National de la Recherche Scientifique (CNRS), Société wallonne des eaux (SWDE), and Département des Sciences et Gestion de L'environnement, Arlon Campus Environnement
Subjects: Environmental Engineering, Wastewater treatment plant, Bivalve, Amphipod, Wastewater, Pollution, Fish, Rivers, Bryophyte, Environmental Chemistry, Animals, [SDV.TOX.ECO]Life Sciences [q-bio]/Toxicology/Ecotoxicology, Waste Management and Disposal, Integrated biomarker response, Ecosystem, Water Pollutants, Chemical, Biological Monitoring, Environmental Monitoring
Abstract: International audience; A biomonitoring approach based on a single model species cannot be representative of the contaminations impacts on the ecosystem overall. As part of the Interreg DIADeM program (“Development of an integrated approach for the diagnosis of the water quality of the River Meuse”), a study was conducted to establish the proof of concept that the use of a multispecies active biomonitoring approach improves diagnostic of aquatic systems. The complementarity of the biomarker responses was tested in four model species belonging to various ecological compartments: the bryophyte Fontinalis antipyretica, the bivalve Dreissena polymorpha, the amphipod Gammarus fossarum and the fish Gasterosteus aculeatus. The species have been caged upstream and downstream from five wastewater treatment plants (WWTPs) in the Meuse watershed. After the exposure, a battery of biomarkers was measured and results were compiled in an Integrated Biomarker Response (IBR) for each species. A multispecies IBR value was then proposed to assess the quality of the receiving environment upstream the WWTPs. The effluent toxicity was variable according to the caged species and the WWTP. However, the calculated IBR were high for all species and upstream sites, suggesting that the water quality was already downgraded upstream the WWTP. This contamination of the receiving environment was confirmed by the multispecies IBR which has allowed to rank the rivers from the less to the most contaminated. This study has demonstrated the interest of the IBR in the assessment of biological impacts of a point-source contamination (WWTP effluent) but also of the receiving environment, thanks to the use of independent references. Moreover, this study has highlighted the complementarity between the different species and has emphasized the interest of this multispecies approach to consider the variability of the species exposition pathway and sensibility as well as the mechanism of contaminants toxicity in the final diagnosis.
Published: 2022

12. Modulation of magnetoencephalography alpha band activity by radiofrequency electromagnetic field depicted in sensor and source space

Author: Jean-Didier Lemaréchal, Jasmina Wallace, Brahim Selmaoui, Lydia Yahia-Cherif, Laurent Hugueville, Christophe Gitton, Institut National de l'Environnement Industriel et des Risques (INERIS), Périnatalité et Risques Toxiques - UMR INERIS_I 1 (PERITOX), Institut National de l'Environnement Industriel et des Risques (INERIS)-Université de Picardie Jules Verne (UPJV)-CHU Amiens-Picardie, Institut du Cerveau = Paris Brain Institute (ICM), Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Institut National de la Santé et de la Recherche Médicale (INSERM)-CHU Pitié-Salpêtrière [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU)-Sorbonne Université (SU)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS), Institut de Neurosciences des Systèmes (INS), Aix Marseille Université (AMU)-Institut National de la Santé et de la Recherche Médicale (INSERM), HAL-SU, Gestionnaire, Université de Picardie Jules Verne (UPJV)-CHU Amiens-Picardie-Institut National de l'Environnement Industriel et des Risques, Institut du Cerveau et de la Moëlle Epinière = Brain and Spine Institute (ICM), Institut National de la Santé et de la Recherche Médicale (INSERM)-CHU Pitié-Salpêtrière [AP-HP], and Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)
Subjects: Adult, Male, Electromagnetic field, Physiology, Science, Rest, Neurophysiology, Alpha (ethology), Article, 030218 nuclear medicine & medical imaging, Young Adult, 03 medical and health sciences, Electromagnetic Fields, 0302 clinical medicine, Nuclear magnetic resonance, Double-Blind Method, Caffeine, medicine, Humans, Heart rate variability, [SDV.NEU] Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC], Saliva, Control parameters, Ocular Physiological Phenomena, Chromatography, High Pressure Liquid, Cerebral Cortex, Physics, Cross-Over Studies, Multidisciplinary, medicine.diagnostic_test, Magnetoencephalography, Crossover study, Alpha band, Modulation, Medicine, [SDV.NEU]Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC], Female, Biomarkers, 030217 neurology & neurosurgery, Neuroscience
Abstract: Several studies reported changes in spontaneous electroencephalogram alpha band activity related to radiofrequency electromagnetic fields, but findings showed both an increase and a decrease of its spectral power or no effect. Here, we studied the alpha band modulation after 900 MHz mobile phone radiofrequency exposure and localized cortical regions involved in these changes, via a magnetoencephalography (MEG) protocol with healthy volunteers in a double-blind, randomized, counterbalanced crossover design. MEG was recorded during eyes open and eyes closed resting-state before and after radiofrequency exposure. Potential confounding factors, known to affect alpha band activity, were assessed as control parameters to limit bias. Entire alpha band, lower and upper alpha sub-bands MEG power spectral densities were estimated in sensor and source space. Biochemistry assays for salivary biomarkers of stress (cortisol, chromogranin-A, alpha amylase), heart rate variability analysis and high-performance liquid chromatography for salivary caffeine concentration were realized. Results in sensor and source space showed a significant modulation of MEG alpha band activity after the radiofrequency exposure, with different involved cortical regions in relation to the eyes condition, probably because of different attention level with open or closed eyes. None of the control parameters reported a statistically significant difference between experimental sessions.
Published: 2021

13. Toxic effects of a mixture of five pharmaceutical drugs assessed using Fontinalis antipyretica Hedw

Author: Khadija Sossey Alaoui, Matthew Saive, Christelle Robert, Sandrine Joachim, Katherine Nott, Bernard Tychon, Sébastien Ronkart, Alain Geffard, Jean-Marc Porcher, Rémy Beaudouin, Marie-Laure Fauconnier, Department of Sciences and Environment Management [Univ. Liège], Université de Liège, Institut National de l'Environnement Industriel et des Risques (INERIS), Stress Environnementaux et BIOsurveillance des milieux aquatiques (SEBIO), Université Le Havre Normandie (ULH), Normandie Université (NU)-Normandie Université (NU)-Université de Reims Champagne-Ardenne (URCA)-SFR Condorcet, Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS)-Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS)-Institut National de l'Environnement Industriel et des Risques (INERIS), Société wallonne des eaux, CER Groupe Marloie, and Gembloux Agro-Bio Tech [Gembloux]
Subjects: Chlorophyll, Fontinalis antipyretica, Antioxidant, Health, Toxicology and Mutagenesis, medicine.medical_treatment, ved/biology.organism_classification_rank.species, 0207 environmental engineering, Bioconcentration, 02 engineering and technology, 010501 environmental sciences, 01 natural sciences, Antioxidants, Environmental pollution, Superoxide dismutase, chemistry.chemical_compound, medicine, GE1-350, Food science, 020701 environmental engineering, 0105 earth and related environmental sciences, biology, Superoxide Dismutase, Chemistry, ved/biology, Chlorophyll A, Public Health, Environmental and Occupational Health, General Medicine, Catalase, Pollution, Glutathione, Bryopsida, 6. Clean water, Environmental sciences, Pharmaceutical Preparations, TD172-193.5, 13. Climate action, Oxidative stress, Bioaccumulation, Toxicity, biology.protein, Pharmaceuticals, Antioxidant enzymes, [SDV.TOX.ECO]Life Sciences [q-bio]/Toxicology/Ecotoxicology, Water Pollutants, Chemical
Abstract: International audience; The potential health risks associated with the pharmaceuticals released into the environment through effluents from sewage treatment plants have become a major cause for concern. Owing to the lack of effective indicators, monitoring the concentration of these pollutants in the aquatic environment is challenging. The aim of this study was to assess the toxicity of a mixture of five pharmaceutical drugs (paracetamol, carbamazepine, diclofenac, irbesartan, and naproxen) using the aquatic moss Fontinalis antipyretica as a bioindicator and bioaccumulator. We examined the effects of the drug mixture on the cellular antioxidant system, chlorophyll content, and morphological traits of F. antipyretica. The plant was exposed for 5 months to three concentrations of the mixture, including the environmental concentration (MX1), and 10-(MX10) and 100-times (MX100) the environmental concentration. The results showed that only carbamazepine and irbesartan were accumulated by the species. The bioconcentration level increased with exposure time, with the maximum uptake at the 4th month of exposure. The increase in bioaccumulation with exposure time was more evident in plants exposed to MX100. Analysis of the activity of antioxidant enzymes showed that superoxide dismutase (SOD, EC 1.15.1.1.) and catalase (EC 1.11.1.6.) were highly sensitive to the drug mixture. The activity of the enzymes was significantly higher in plants exposed to MX100; however, the activity of guaiacol peroxidase (GPX, EC 1.11.1.7.) was not significantly affected. Plants exposed to MX10 and MX100 had significantly lower total chlorophyll content and chlorophyll a/b ratio compared with those of plants in the control group; however, photosynthetic activity was restored after 5 months of exposure. The morphological characteristics of F. antipyretica were less sensitive to the treatment conditions.
Published: 2021

14. Nose-only inhalations of high-dose alumina nanoparticles/hydrogen chloride gas mixtures induce strong pulmonary pro-inflammatory response: a pilot study

Author: Anne-Laure Favier, Francisca Fargeau, Emmanuel Flahaut, Emmanuel Peyret, Alexandra Bourgois, Sylvie Renault, Ghislaine Lacroix, Samir Dekali, Dominique Saurat, Suzanne De Araujo, Christine Frederic, Nathalie Guitard, Aurélie Servonnet, Alexandre Boyard, Sabine François, Institut de Recherche Biomédicale des Armées (IRBA), Institut National de l'Environnement Industriel et des Risques (INERIS), Centre interuniversitaire de recherche et d'ingenierie des matériaux (CIRIMAT), Centre National de la Recherche Scientifique (CNRS)-Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Institut de Chimie du CNRS (INC), The French Government of Defense Procurement Agency, TheDirection Générale de l’Armement (DGA), The Agence de l’InnovationDéfense (AID), France, research program PROPERGOL fundedthis work., PROPERGOL, Centre National de la Recherche Scientifique - CNRS (FRANCE), Institut National de l'Environnement Industriel et des Risques - INERIS (FRANCE), Institut National Polytechnique de Toulouse - Toulouse INP (FRANCE), Institut de Recherche Biomédicale des Armées - IRBA (FRANCE), Université Toulouse III - Paul Sabatier - UT3 (FRANCE), and Centre Interuniversitaire de Recherche et d'Ingénierie des Matériaux - CIRIMAT (Toulouse, France)
Subjects: Male, Pulmonary toxicity, Health, Toxicology and Mutagenesis, Nanoparticle, Pilot Projects, 010501 environmental sciences, Pharmacology, Toxicology, [SDV.MHEP.PSR]Life Sciences [q-bio]/Human health and pathology/Pulmonology and respiratory tract, 01 natural sciences, chemistry.chemical_compound, alumina nanoparticles, Physique Médicale, Alumina nanoparticles, Aluminum Oxide, Hydrogen chloride, Lung, 0303 health sciences, Inhalation Exposure, Inhalation, medicine.diagnostic_test, Chemistry, respiratory system, 3. Good health, medicine.anatomical_structure, [SDV.TOX]Life Sciences [q-bio]/Toxicology, nose-only inhalation, Bronchoalveolar Lavage Fluid, hydrogen chloride, Matériaux, Combustion aerosols, 03 medical and health sciences, medicine, Animals, Rats, Wistar, Nose-only inhalation, 030304 developmental biology, 0105 earth and related environmental sciences, [SDV.EE.SANT]Life Sciences [q-bio]/Ecology, environment/Health, Aerosols, Pneumonia, Aerosol, Rats, Cytolysis, Bronchoalveolar lavage, combustion aerosols, Pulmonary inflammation, Nanoparticles, Hydrochloric Acid
Abstract: International audience; Objective: Solid composite propellants combustion, in aerospace and defense fields, can lead to complex aerosols emission containing high concentrations of alumina nanoparticles (Al2O3 NPs) and hydrogen chloride gas (HClg). Exposure to these mixtures by inhalation is thus possible but literature data toward their pulmonary toxicity are missing. To specify hazards resulting from these combustion aerosols, a pilot study was implemented.Materials and methods: Male Wistar rats were nose-only exposed to Al2O3 NPs (primary size 13 nm, 10 g/L suspension leading to 20.0–22.1 mg/m3 aerosol) and/or to HClg aerosols (5 ppm target concentration) following two exposure scenarios (single exposures (SE) or repeated exposures (RE)). Bronchoalveolar lavage fluids (BALF) content and lungs histopathology were analyzed 24 h after exposures.Results: Repeated co-exposures increased total proteins and LDH concentrations in BALF indicating alveolar–capillary barrier permeabilization and cytolysis. Early pulmonary inflammation was induced after RE to Al2O3 NPs ± HClg resulting in PMN, TNF-α, IL-1β, and GRO/KC increases in BALF. Both exposure scenarios resulted in pulmonary histopathological lesions (vascular congestions, bronchial pre-exfoliations, vascular and interalveolar septum edemas). Lung oxidative damages were observed in situ following SE.Conclusion:Observed biological effects are dependent on both aerosol content and exposure scenario. Results showed an important pro-inflammatory effect of Al2O3 NPs/HClg mixtures on the lungs of rat 24 h after exposure. This pilot study raises concerns toward potential long-term pulmonary toxicity of combustion aerosols and highlights the importance for further studies to be led in order to define dose limitations and exposure thresholds for risk management at the work place.
Published: 2021

15. Evaluation of Placental Transfer and Tissue Distribution of cis- and Trans-Permethrin in Pregnant Rats and Fetuses Using a Physiological-Based Pharmacokinetic Model

Author: Stéphane Personne, Céline Brochot, Paulo Marcelo, Aurélie Corona, Sophie Desmots, Franck Robidel, Anthony Lecomte, Véronique Bach, Florence Zeman, Institut National de l'Environnement Industriel et des Risques (INERIS), Périnatalité et Risques Toxiques - UMR INERIS_I 1 (PERITOX), Université de Picardie Jules Verne (UPJV)-CHU Amiens-Picardie-Institut National de l'Environnement Industriel et des Risques, Plateforme d’Ingénierie Cellulaire et Analyses des Protéines (ICAP) (ICAP), and Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS)
Subjects: permethrin, brain, Physiology, 010501 environmental sciences, PBPK model, 01 natural sciences, Pediatrics, Intestinal absorption, RJ1-570, 03 medical and health sciences, chemistry.chemical_compound, pyrethroids, Meconium, Placenta, parasitic diseases, medicine, rat, Original Research, 030304 developmental biology, 0105 earth and related environmental sciences, 0303 health sciences, Pregnancy, Fetus, Pyrethroid, business.industry, pesticides, medicine.disease, 3. Good health, fetus, medicine.anatomical_structure, chemistry, [SDV.TOX]Life Sciences [q-bio]/Toxicology, Pediatrics, Perinatology and Child Health, Gestation, pregnancy, business, Permethrin, medicine.drug
Abstract: Biomonitoring studies have highlighted the exposure of pregnant women to pyrethroids based on the measurement of their metabolites in urine. Pyrethroids can cross the placental barrier and be distributed in the fetus as some pyrethroids were also measured in the meconium of newborns. Prenatal exposure to pyrethroids is suspected to alter the neurodevelopment of children, and animal studies have shown that early life exposure to permethrin, one of the most commonly used pyrethroid in household applications, can alter the brain development. This study aimed to characterize the fetal permethrin exposure throughout gestation in rats. We developed a pregnancy physiologically based pharmacokinetic (pPBPK) model that describes the maternal and fetal kinetics of the cis- and trans- isomers of permethrin during the whole gestation period. Pregnant Sprague–Dawley rats were exposed daily to permethrin (50 mg/kg) by oral route from the start of gestation to day 20. Permethrin isomers were quantified in the feces, kidney, mammary gland, fat, and placenta in dams and in both maternal and fetal blood, brain, and liver. Cis- and trans-permethrin were quantified in fetal blood and tissues, with higher concentrations for the cis-isomer. The pPBPK model was fitted to the toxicokinetic maternal and fetal data in a Bayesian framework. Several parameters were adjusted, such as hepatic clearances, partition coefficients, and intestinal absorption. Our work allowed to estimate the prenatal exposure to permethrin in rats, especially in the fetal brain, and to quantitatively estimate the placental transfer. These transfers could be extrapolated to humans and be incorporated in a human pPBPK model to estimate the fetal exposure to permethrin from biomonitoring data.
Published: 2021

16. Integrated multi-biomarker responses of juvenile rainbow trout (Oncorhynchus mykiss) to an environmentally relevant pharmaceutical mixture

Author: Syaghalirwa N.M. Mandiki, Christelle Robert, Sébastien Baekelandt, Stéphane Betoulle, Mélodie Schmitz, Katherine Nott, Mahaut Beghin, Sébastien Ronkart, Patrick Kestemont, Erin Gillet, Jean-Marc Porcher, Olivier Palluel, Université de Namur [Namur] (UNamur), Stress Environnementaux et BIOsurveillance des milieux aquatiques (SEBIO), Université Le Havre Normandie (ULH), Normandie Université (NU)-Normandie Université (NU)-Université de Reims Champagne-Ardenne (URCA)-SFR Condorcet, Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS)-Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS)-Institut National de l'Environnement Industriel et des Risques (INERIS), Institut National de l'Environnement Industriel et des Risques (INERIS), Société wallonne des eaux, and Centre d'Economie Rurale
Subjects: Antioxidant, Health, Toxicology and Mutagenesis, medicine.medical_treatment, 0211 other engineering and technologies, 02 engineering and technology, 010501 environmental sciences, 01 natural sciences, Environmental pollution, chemistry.chemical_compound, Naproxen, Belgium, GE1-350, Food science, Chronic toxicity, Chemistry, Aquatic ecosystem, Brain, Drugs, General Medicine, Pollution, 6. Clean water, Cocktail, Carbamazepine, Liver, TD172-193.5, Oncorhynchus mykiss, Neurotoxicity Syndromes, [SDV.TOX.ECO]Life Sciences [q-bio]/Toxicology/Ecotoxicology, Lysozyme, Serotonin, Diclofenac, Rivers, IBR, medicine, Neurotoxicity, Juvenile, Animals, 14. Life underwater, 0105 earth and related environmental sciences, Acetaminophen, Pollutant, 021110 strategic, defence & security studies, Public Health, Environmental and Occupational Health, Immunity, Irbesartan, medicine.disease, Environmental sciences, Fish, 13. Climate action, Rainbow trout, Lysosomes, Biomarkers, Water Pollutants, Chemical
Abstract: International audience; Pharmaceuticals are emerging pollutants of concern for aquatic ecosystems where they are occurring in complex mixtures. In the present study, the chronic toxicity of an environmentally relevant pharmaceutical mixture on juvenile rainbow trout (Oncorhynchus mykiss) was investigated. Five pharmaceuticals (paracetamol, carbamazepine, diclofenac, naproxen and irbesartan) were selected based on their detection frequency and concentration levels in the Meuse river (Belgium). Fish were exposed for 42 days to three different concentrations of the mixture, the median one detected in the Meuse river, 10-times and 100-times this concentration. Effects on the nervous, immune, antioxidant, and detoxification systems were evaluated throughout the exposure period and their response standardized using the Integrated Biomarker Response (IBRv2) index. IBRv2 scores increased over time in the fish exposed to the highest concentration. After 42 days, fish exposed to the highest concentration displayed significantly higher levels in lysozyme activity (p < 0.01). The mixture also caused significant changes in brain serotonin turnover (p < 0.05). In short, our results indicate that the subchronic waterborne exposure to a pharmaceutical mixture commonly occurring in freshwater ecosystems may affect the neuroendocrine and immune systems of juvenile rainbow trout.
Published: 2021

17. Antidepressants Modify Cryptic Behavior in Juvenile Cuttlefish at Environmentally Realistic Concentrations

Author: Cécile Bellanger, Thomas Knigge, Apolline Chabenat, Université de Caen Normandie (UNICAEN), Normandie Université (NU), Ethologie animale et humaine (EthoS), Normandie Université (NU)-Normandie Université (NU)-Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-Centre National de la Recherche Scientifique (CNRS), Stress Environnementaux et BIOsurveillance des milieux aquatiques (SEBIO), Université Le Havre Normandie (ULH), Normandie Université (NU)-Normandie Université (NU)-Université de Reims Champagne-Ardenne (URCA)-SFR Condorcet, Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS)-Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS)-Institut National de l'Environnement Industriel et des Risques (INERIS), Normandy Region, Normandie Université (NU)-Normandie Université (NU)-Université de Rennes (UR)-Centre National de la Recherche Scientifique (CNRS), Institut National de l'Environnement Industriel et des Risques (INERIS)-Université de Reims Champagne-Ardenne (URCA)-Université Le Havre Normandie (ULH), Normandie Université (NU)-Normandie Université (NU)-SFR Condorcet, and Université de Reims Champagne-Ardenne (URCA)-Centre National de la Recherche Scientifique (CNRS)-Université de Reims Champagne-Ardenne (URCA)-Centre National de la Recherche Scientifique (CNRS)
Subjects: Cuttlefish, Camouflage, Sepia, Health, Toxicology and Mutagenesis, Zoology, Venlafaxine, 010501 environmental sciences, Development, Sepia officinalis, 01 natural sciences, Cephalopod, 03 medical and health sciences, Fluoxetine, medicine, Animals, Environmental Chemistry, Juvenile, [CHIM]Chemical Sciences, 14. Life underwater, 030304 developmental biology, 0105 earth and related environmental sciences, 0303 health sciences, Behavior, Animal, biology, Body color changes, Decapodiformes, Venlafaxine Hydrochloride, biology.organism_classification, Antidepressive Agents, [SDV.TOX]Life Sciences [q-bio]/Toxicology, Officinalis, [SDE]Environmental Sciences, medicine.drug
Abstract: Contamination of the marine environment by antidepressants may affect neurophysiological processes in nontarget organisms, such as the common cuttlefish, Sepia officinalis. The present study tested whether environmentally realistic concentrations of antidepressants, that is, fluoxetine alone (5 ng L-1 ) or cumulated with venlafaxine (2.5 or 5 ng L-1 ), affect camouflage in newly hatched cuttlefish. The results show that antidepressants improved uniform body patterns, whereas disruptive body patterns were not affected. Environ Toxicol Chem 2021;40:2571-2577. © 2021 SETAC.
Published: 2021

18. A meta-analysis of ecotoxicological models used for plant protection product risk assessment before their placing on the market

Author: Floriane Larras, Rémy Beaudouin, Philippe Berny, Sandrine Charles, Arnaud Chaumot, Marie-France Corio-Costet, Isabelle Doussan, Céline Pelosi, Sophie Leenhardt, Laure Mamy, Direction de l'Expertise scientifique collective, de la Prospective et des Etudes, Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Stress Environnementaux et BIOsurveillance des milieux aquatiques (SEBIO), Institut National de l'Environnement Industriel et des Risques (INERIS)-Université de Reims Champagne-Ardenne (URCA)-Université Le Havre Normandie (ULH), Normandie Université (NU)-Normandie Université (NU)-SFR Condorcet, Université de Reims Champagne-Ardenne (URCA)-Centre National de la Recherche Scientifique (CNRS)-Université de Reims Champagne-Ardenne (URCA)-Centre National de la Recherche Scientifique (CNRS), Institut National de l'Environnement Industriel et des Risques (INERIS), VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS), Interactions Cellules Environnement - UR (ICE), Laboratoire de Biométrie et Biologie Evolutive - UMR 5558 (LBBE), Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut National de Recherche en Informatique et en Automatique (Inria)-VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-Centre National de la Recherche Scientifique (CNRS), RiverLy - Fonctionnement des hydrosystèmes (RiverLy), Santé et agroécologie du vignoble (UMR SAVE), Université de Bordeaux (UB)-Institut des Sciences de la Vigne et du Vin (ISVV)-Ecole Nationale Supérieure des Sciences Agronomiques de Bordeaux-Aquitaine (Bordeaux Sciences Agro)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Groupe de Recherche en Droit, Economie et Gestion (GREDEG), Université Nice Sophia Antipolis (1965 - 2019) (UNS), COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-Centre National de la Recherche Scientifique (CNRS)-Université Côte d'Azur (UCA), Environnement Méditerranéen et Modélisation des Agro-Hydrosystèmes (EMMAH), Avignon Université (AU)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Ecologie fonctionnelle et écotoxicologie des agroécosystèmes (ECOSYS), and AgroParisTech-Université Paris-Saclay-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)
Subjects: Mammals, Environmental Engineering, Biodiversity, Plants, Ecotoxicology, Risk Assessment, Pesticide package, Pollution, Regulation procedures, Magnoliopsida, [SDE]Environmental Sciences, Animals, Humans, Environmental Chemistry, Pesticide approval, European Union, Ecological risk assessment, Waste Management and Disposal
Abstract: International audience; Before their placing on the market, the safety of plant protection products (PPP) towards both human and animal health, and the environment has to be assessed using experimental and modelling approaches. Models are crucial tools for PPP risk assessment and some even help to avoid animal testing. This review investigated the use of modelling approaches in the ecotoxicology section of PPP active substance assessment reports prepared by the authorities and opened to consultation from 2011 to 2021 in the European Union. Seven categories of models (Structure-Activity, ToxicoKinetic, ToxicoKinetic-ToxicoDynamic, Species Sensitivity Distribution, population, community, and mixture) were searched for into the reports of 317 active substances. At least one model category was found for 44 % of the investigated active substances. The most detected models were Species Sensitivity Distribution, Structure-Activity and ToxicoKinetic for 27, 21 and 15 % of the active substances, respectively. The use of modelling was of particular importance for conventional active substances such as sulfonylurea or carbamates contrary to microorganisms and plant derived substances. This review also highlighted a strong imbalance in model usage among the biological groups considered in the European Regulation (EC) No 1107/2009. For example, models were more often used for aquatic than for terrestrial organisms (e.g., birds, mammals). Finally, a gap between the set of models used in reports and those existing in the literature was observed highlighting the need for the implementation of more sophisticated models into PPP regulation.
Published: 2022

19. In situ use of bivalves and passive samplers to reveal water contamination by microcystins along a freshwater-marine continuum in France

Author: Myriam Bormans, Zouher Amzil, Emilie Lance, Véronique Savar, Alexandra Lepoutre, Elise Robert, Stress Environnementaux et BIOsurveillance des milieux aquatiques (SEBIO), Université Le Havre Normandie (ULH), Normandie Université (NU)-Normandie Université (NU)-Université de Reims Champagne-Ardenne (URCA)-SFR Condorcet, Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS)-Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS)-Institut National de l'Environnement Industriel et des Risques (INERIS), Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER), Ecosystèmes, biodiversité, évolution [Rennes] (ECOBIO), Centre National de la Recherche Scientifique (CNRS)-Observatoire des Sciences de l'Univers de Rennes (OSUR)-Institut Ecologie et Environnement (INEE), Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS)-Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES), This work was funded by the French National Research Program for Environmental and Occupational Health of the French Agency for Food, Environmental and Occupational Health & Safety (ANSES 2015/1/191, Bieautox program)., Institut National de l'Environnement Industriel et des Risques (INERIS)-Université de Reims Champagne-Ardenne (URCA)-Université Le Havre Normandie (ULH), Normandie Université (NU)-Normandie Université (NU)-SFR Condorcet, Université de Reims Champagne-Ardenne (URCA)-Centre National de la Recherche Scientifique (CNRS)-Université de Reims Champagne-Ardenne (URCA)-Centre National de la Recherche Scientifique (CNRS), Université de Rennes (UR)-Institut Ecologie et Environnement (INEE), Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS)-Observatoire des Sciences de l'Univers de Rennes (OSUR), and Université de Rennes (UR)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Rennes 2 (UR2)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Rennes 2 (UR2)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Centre National de la Recherche Scientifique (CNRS)
Subjects: 0106 biological sciences, In situ, Cyanobacteria, biosurvey, Environmental Engineering, Microcystins, Cyanotoxins, [SDV]Life Sciences [q-bio], Fresh Water, 01 natural sciences, 03 medical and health sciences, cyanotoxins, Phytoplankton, Animals, Humans, 14. Life underwater, Waste Management and Disposal, Ecosystem, 030304 developmental biology, Water Science and Technology, Civil and Structural Engineering, molluscs, 0303 health sciences, geography, geography.geographical_feature_category, biology, 010604 marine biology & hydrobiology, Ecological Modeling, Aquatic ecosystem, Solid phase adsorption toxin tracking (SPATT), fungi, Water, Estuary, Molluscs, Mussel, Contamination, biology.organism_classification, Pollution, 6. Clean water, Mytilus, Bivalvia, solid phase adsorption toxin tracking (SPATT), Biosurvey, Environmental chemistry, Environmental science
Abstract: International audience; Cyanobacteria are a potential threat to aquatic ecosystems and human health because of their ability to produce cyanotoxins, such as microcystins (MCs). MCs are regularly monitored in fresh waters, but rarely in estuarine and marine waters despite the possibility of their downstream export. Over a period of two years, we monthly analyzed intracellular (in phytoplankton) and extracellular (dissolved in water) MCs at five stations along a river continuum from a freshwater reservoir with ongoing cyanobacterial blooms to the coast of Brittany, France. MCs were quantified using two integrative samplers placed at each site: solid phase adsorption toxin tracking (SPATT) samplers for collecting extracellular MCs and caged mussels (Anodonta anatina and Mytilus edulis) filter-feeding on MC-producing cyanobacteria. The MC transfer was demonstrated each year during five months at estuarine sites and sporadically at the marine outlet. SPATT samplers integrated extracellular MCs, notably at low environmental concentrations (0.2 µg/L) and with the same variant profile as in water. The mussel A. anatina highlighted the presence of MCs including at intracellular concentrations below 1 µg/L. M. edulis more efficiently revealed the MC transfer at estuarine sites than water samplings. Bivalves showed the same MC variant profile as phytoplankton samples, but with differential accumulation capacities between the variants and the two species. Using SPATT or bivalves can give a more accurate assessment of the contamination level of a freshwater-marine continuum, in which the MC transfer can be episodic. MC content in M. edulis represents a potent threat to human health if considering updated French guideline values, and particularly the total (free and protein-bound) MC content, highlighting the necessity to include cyanotoxins in the monitoring of seafood originating from estuarine areas.
Published: 2021

20. Toxicology and Biodegradability of a Phthalate-Free and Bio-Based Novel Plasticizer

Author: A. Chentouf, Fabrice Nesslany, J.-M. Raimbault, S. Mortier, D. Wils, Laure Chabot, Franck Gondelle, M.-L. Sola, S. Simar-Mentières, Pascal Pandard, Université de Lille, CHU Lille, Institut Pasteur de Lille, Laboratoire de toxicologie génétique [Lille], European Research Biology Center [ERBC], Institut National de l'Environnement Industriel et des Risques [INERIS], Roquette Frères, Réseau International des Instituts Pasteur (RIIP)-Réseau International des Instituts Pasteur (RIIP), European Research Biology Center (ERBC), Institut National de l'Environnement Industriel et des Risques (INERIS), and The study was funded by ROQUETTE Frères.
Subjects: Pharmacology, Isosorbide, Article Subject, Plasticizer, Phthalate, Bio based, 010501 environmental sciences, Biodegradation, Toxicology, Pulp and paper industry, 030226 pharmacology & pharmacy, 01 natural sciences, 03 medical and health sciences, Polyvinyl chloride, chemistry.chemical_compound, 0302 clinical medicine, chemistry, [SDV.TOX]Life Sciences [q-bio]/Toxicology, RA1190-1270, Toxicology. Poisons, medicine, Research Article, 0105 earth and related environmental sciences, medicine.drug
Abstract: Phthalate esters, mainly di-ethylhexylphthalate (DEHP), represent a class of chemicals primarily used as plasticizers for polyvinyl chloride in a wide range of domestic and industrial applications. These phthalate esters are low-toxicity environmental contaminants. To address these drawbacks, POLYSORB® ID 37, a blend of diesters obtained from esterification of isosorbide with plant-based fatty acids, was developed. The company can now offer PVC manufacturers a new product which competes with phthalates and other such chemicals. The market for plasticizers is very important, and ROQUETTE intends to provide a more sustainable and safer product. Isosorbide diester is bio-based (made from glucose and vegetable fatty acids). This plasticizer is registered in REACH regulation for high volumes (>1000 T/year). Risk assessment was obtained by conducting a wide range of biodegradability and toxicological protocols, using rodent models, according to established guidelines. Overall, all of the toxicological and biodegradability studies demonstrated that POLYSORB® ID 37 is nontoxic to mammalian life and is readily biodegradable.
Published: 2021

21. Toward waste valorization by converting bioethanol production residues into nanoparticles and nanocomposite films

Author: Guillaume Riviere, Pascal Pandard, Xun Liao, Stéphanie Baumberger, Florian Pion, Guy Marlair, Hanna Koivula, Thangavelu Jayabalan, Muhammad Farooq, Monika Österberg, Mika Henrikki Sipponen, Department of Food and Nutrition, Helsinki Institute of Sustainability Science (HELSUS), Aalto University, Institut Jean-Pierre Bourgin (IJPB), AgroParisTech-Université Paris-Saclay-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), University of Helsinki, Institut National de l'Environnement Industriel et des Risques (INERIS), Ecole Polytechnique Fédérale de Lausanne (EPFL), Quantis, H2020 BBI-JU, FinnCERES Materials Bioeconomy Ecosystem, Aalto University Bioeconomy Facilities, European Project: 720303,Zelcor, Helsingin yliopisto = Helsingfors universitet = University of Helsinki, Bioproduct Chemistry, Université Paris-Saclay, Institut national de l'environnement industriel et des risques, Department of Bioproducts and Biosystems, and Aalto-yliopisto
Subjects: [SDV.BIO]Life Sciences [q-bio]/Biotechnology, LIGNIN NANOPARTICLES, FABRICATION, Nanoparticle, 02 engineering and technology, Raw material, 010402 general chemistry, 7. Clean energy, 01 natural sciences, NANOSTRUCTURES, Industrial and Manufacturing Engineering, Nanomaterials, [SPI.MAT]Engineering Sciences [physics]/Materials, BIOMASS, MECHANISMS, CUO, chemistry.chemical_compound, Hydrolysis, Life cycle assessment, lifecycle assessment, ANTIOXIDANT, ZNO, Lignin, lignin nanoparticles, WATER, General Materials Science, Ecotoxicity, Cellulose, [SPI.NANO]Engineering Sciences [physics]/Micro and nanotechnologies/Microelectronics, Waste Management and Disposal, biorefinery, Nanocomposite, Renewable Energy, Sustainability and the Environment, ecotoxicity, cellulose nanofibrils, Lignocellulosic nanofibrils, CELLULOSE NANOFIBRILS, 021001 nanoscience & nanotechnology, 0104 chemical sciences, lignocellulosic nanofibrils, Biorefinery, Cellulose fiber, chemistry, Chemical engineering, 13. Climate action, 221 Nano-technology, 0210 nano-technology
Abstract: A “waste-valorization” approach was developed to transform recalcitrant hydrolysis lignin (HL) from second-generationbioethanol productioninto multifunctional bio-based products. The hydrolysis lignin (HL) was extracted with aqueousacetone, yielding two fractions enriched in lignin and cellulose, respectively. The soluble hydrolysis lignin (SHL) was converted into anionic and cationic colloidal lignin particles (CLPs and c-CLPs). The insoluble cellulose-rich fraction was transformed intolignocellulosicnanofibrils that were further combined with CLPs or c-CLPs to obtainnanocomposite filmswithtailored mechanical properties,oxygen permeabilityand antioxidant properties. To enable prospective applications of lignin in nanocomposite films and beyond, CLPs and c-CLPs were also produced from a soda lignin (SL) and the influence of the lignin type on the particle size and ecotoxicity was evaluated. Finally, the carbon footprint of the entire process from hydrolysis lignin to films was assessed and an integration to industrial scale was considered to reduce the energy consumption. While most previous work utilizes purified lignin and pristine and often purified cellulose fibers to produce nanomaterials, this work provides aproof of conceptfor utilizing the recalcitrant lignin-rich side stream of thebioethanolprocess as raw material for functional nanomaterials and renewable composites.
Published: 2021

22. Bioanalytical Characterization and Source Identification of a High Glucocorticoid Contamination in a River Under Urban and Industrial Pressures

Author: Chardon, Clémence, Gardia-Parège, Caroline, Maillot-Maréchal, Emmanuelle, Debret, Maxime, Budzinski, Hélène, Ait-Aissa, Selim, Institut National de l'Environnement Industriel et des Risques (INERIS), Stress Environnementaux et BIOsurveillance des milieux aquatiques (SEBIO), Université Le Havre Normandie (ULH), Normandie Université (NU)-Normandie Université (NU)-Université de Reims Champagne-Ardenne (URCA)-SFR Condorcet, Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS)-Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS)-Institut National de l'Environnement Industriel et des Risques (INERIS), Environnements et Paléoenvironnements OCéaniques (EPOC), Observatoire aquitain des sciences de l'univers (OASU), Université Sciences et Technologies - Bordeaux 1-Institut national des sciences de l'Univers (INSU - CNRS)-Centre National de la Recherche Scientifique (CNRS)-Université Sciences et Technologies - Bordeaux 1-Institut national des sciences de l'Univers (INSU - CNRS)-Centre National de la Recherche Scientifique (CNRS)-École pratique des hautes études (EPHE), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Centre National de la Recherche Scientifique (CNRS), Morphodynamique Continentale et Côtière (M2C), Université de Caen Normandie (UNICAEN), Normandie Université (NU)-Normandie Université (NU)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Rouen Normandie (UNIROUEN), Normandie Université (NU)-Centre National de la Recherche Scientifique (CNRS), and Civs, Gestionnaire
Subjects: [SDV.TOX.ECO] Life Sciences [q-bio]/Toxicology/Ecotoxicology, [SDV.TOX.ECO]Life Sciences [q-bio]/Toxicology/Ecotoxicology
Abstract: International audience; Synthetic glucocorticoids (GCs) are a group of steroids that are massively used as anti-inflammatory drugs to treat a broad spectrum of deseases such as asthma or arthritis. They may enter surface waters via urban, hospital and/or pharmaceutical industry effluents and were already detected in surface waters at concentrations ranging from < 1 to tens of ng/L. In organisms, GCs are designed to act through the glucocorticoid receptor (GR) and some studies have reported adverse effects on aquatic vertebrates, such as fish. Overall, there is still a need for knowledge on the sources and driver compounds for GR activity in different contexts. In the frame of a French national survey of EDCs in surface waters using in vitro bioassays, we previously identified a hotspot river site with high GR activity. To further identify the source(s) and the nature of this contamination, an investigative monitoring based on bioassay (MDA-Kb2) and chemical analyses was implemented in the current study. A contamination mapping was established on the basis of bioassay data, along a 40 km streatch upstream and downstream of the contaminated site allowing identification of the discharge of the contamination in the river and of its highly probable industrial origin in the sewerage network, although the contribution of waste waters from a hospital could not be excluded. By using a suspect screening approach based on HR-MS, fluticasone propionate as well as few other GCs were identified in the samples. Mass balance analysis based on quantitave chemical analysis confirmed fluticasone propionate main role in the measured GR activity. The high mobility and persistence of this contamination points out possible high environmental concern in terms of its fate and hazard for aquatic organisms.
Published: 2021

23. Modeling Acetylcholine Esterase Inhibition Resulting From Exposure to a Mixture of Atrazine and Chlorpyrifos Using a Physiologically Based Kinetic Model in Fish

Author: Mit, Corentin, Tebby, Cléo, Gueganno, Tristan, Bado-Nilles, Anne, Beaudouin, Rémy, Institut National de l'Environnement Industriel et des Risques (INERIS), Stress Environnementaux et BIOsurveillance des milieux aquatiques (SEBIO), Université Le Havre Normandie (ULH), Normandie Université (NU)-Normandie Université (NU)-Université de Reims Champagne-Ardenne (URCA)-SFR Condorcet, and Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS)-Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS)-Institut National de l'Environnement Industriel et des Risques (INERIS)
Subjects: [SDV.TOX.ECO]Life Sciences [q-bio]/Toxicology/Ecotoxicology
Abstract: International audience; Aquatic organisms are exposed to mixtures of chemicals that may interact. Mixtures of atrazine (ATR) and chlorpyrifos (CPF) may elicit synergic effects on the permanent inhibition of acetylcholinesterase (AChE) in certain aquatic organisms, causing severe damage. Mechanistic mathematical models of toxicokinetics and toxicodynamics (TD) may be used to better characterize and understand the interactions of these two chemicals. In this study, a previously published generic physiologically-based toxicokinetic (PBTK) model for fish was adapted to ATR and CPF. A sub-model of the kinetics of one of the main metabolites of CPF, chlorpyrifos-oxon (CPF-oxon), was included, as well as a TD model. Inhibition of two esterases, AChE and carboxylesterase, by ATR, CPF and CPF-oxon, was modeled using TD modeling of quantities of total and inactive esterases. Specific attention was given to the parameterization and calibration of the model to accurately predict the concentration and effects observed in the fish using Bayesian inference and published data from fathead minnow (Pimephales promelas), zebrafish (Danio rerio) and common carp (Cyprinus carpio L.). A PBTK-TD for mixtures was used to predict dose-response relationships for comparison with available adult fish data. Synergistic effects of a joint exposure to ATR and CPF could not be demonstrated in adult fish.
Published: 2021

24. Multi-Biomarkers Approach in Three-Spined Stickleback (Gasterosteus Aculeatus, L.) Using an ENvironmental Biomonitoring Analysis : Genotoxic Biomarkers Integration

Author: Cant, Amélie, Bonnard, Marc, Porcher, Jean-Marc, Prygiel, Jean, Catteau, Audrey, Delahaut, Laurence, Palluel, Olivier, Turies, Cyril, Geffard, Alain, Bado-Nilles, Anne, Institut National de l'Environnement Industriel et des Risques (INERIS), Stress Environnementaux et BIOsurveillance des milieux aquatiques (SEBIO), Université Le Havre Normandie (ULH), Normandie Université (NU)-Normandie Université (NU)-Université de Reims Champagne-Ardenne (URCA)-SFR Condorcet, Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS)-Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS)-Institut National de l'Environnement Industriel et des Risques (INERIS), and Agence de l'Eau Artois-Picardie
Subjects: [SDV.TOX.ECO]Life Sciences [q-bio]/Toxicology/Ecotoxicology
Abstract: International audience; In order to improve the diagnosis of environmental contamination of freshwater, multi-biomarkers approaches have been developed to better reflect the physiological state of an organism and potentially detect exposures to xenobiotics. Genotoxic biomarkers seem to be quite relevant due to the multitude of substances interacting with the DNA molecule which can affect its integrity in somatic and germ cells. Among the existing tools used to highlight the genotoxicity on fish erythrocytes, the alkaline comet assay (SCGE) is the most widely used method to detect primary DNA strand breaks and flow cytometry (FCM) is becoming a fast and convenient tool to notice chromosomal damages for aquatic biomonitoring. The present work proposes to evaluate the relevance to include two genotoxic biomarkers (primary DNA strand breaks and chromosomal damages) in the existing battery of multi-biomarkers developed on three-spined stickleback (Gasterosteus aculeatus). After 21 days of exposure, biological samples were recovered from sticklebacks caged in six sites in Northern France to constitute the battery. The inclusion of genotoxicity biomarkers seems to bring a little improvement to the discriminating power of sites as obtained by a Principal Component Analysis (PCA) explaining 30.8% of the variance, although they provide a complementary information on the toxicity of sites. The “Rhonelle” river was the most affected by primary DNA strand breaks measured in fish but this parameter was not well represented on axis, in opposition to fish caged in “Deule” and “Sensée Bouchain” sites showing lower levels of chromosomal damages, in accordance with the first component axis of the PCA.
Published: 2021

25. Development of a Physiologically-Based Toxicokinetic Model of BPA in Fish Including Its Two Main Metabolites, Bpa-Glucuronide and Bpa-Sulfate

Author: Mit, Corentin, Bado-Nilles, Anne, Beaudouin, Rémy, Institut National de l'Environnement Industriel et des Risques (INERIS), Stress Environnementaux et BIOsurveillance des milieux aquatiques (SEBIO), Université Le Havre Normandie (ULH), Normandie Université (NU)-Normandie Université (NU)-Université de Reims Champagne-Ardenne (URCA)-SFR Condorcet, and Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS)-Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS)-Institut National de l'Environnement Industriel et des Risques (INERIS)
Subjects: endocrine system, animal structures, urogenital system, [SDV.TOX.ECO]Life Sciences [q-bio]/Toxicology/Ecotoxicology, hormones, hormone substitutes, and hormone antagonists
Abstract: International audience; Physiologically-based toxicokinetic models (PBTK) are useful tools to improve understanding of the fate of a chemical inside an organism. Recently, Grech et al. (2019) proposed a generic PBTK model for four different fish, including zebrafish (Danio rerio), threespine stickleback (Gasterosteus aculeatus), rainbow trout (Oncorhynchus mykiss) and fathead minnow (Pimephales promelas). Meanwhile, bisphenol A (BPA) is considered as very high concern substance due to its endocrine disruptor function, its high production volume and its persistence in the aquatic environment. In this work, the PBTK will be adapted to accurately model the ADME processes (absorption, distribution, metabolization and excretion) of BPA. A particular effort will be carried out to predict internal concentration of its two main metabolites, BPA-monoglucuronide (BPA-g) and BPA-monosulfate (BPA-s), in various organs. In vitro data from zebrafish, rat (Sprague-Dawley) and mouse (CD-1) hepatocytes will be used to calculate Michaelis-Menten parameters related to metabolism. Moreover, TK data in zebrafish and rainbow trout for BPA, BPA-G and BPA-S in different organs will be retrieved from literature to calibrate the model. The objective will be to accurately predict TK data measured in vivo in external datasets. Further development will later add TD sub-models to predict the dynamic of biomarkers measured in organs.
Published: 2021

26. Development of an Untargeted Metabolomics Approach Based on LC-(ESI)-HRMS, Applied to Three-Spined Sticklebacks Exposed to Diclofenac

Author: Lebeau, Emmanuelle, Daniele, Gaëlle, Fildier, Aurélie, Turies, Cyril, Bado-Nilles, Anne, Porcher, Jean-Marc, Dedourge-Geffard, Odile, Geffard, Alain, Vulliet, Emmanuelle, Université de Reims Champagne-Ardenne (URCA), Institut des Sciences Analytiques (ISA), Institut de Chimie du CNRS (INC)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS), Institut National de l'Environnement Industriel et des Risques (INERIS), Stress Environnementaux et BIOsurveillance des milieux aquatiques (SEBIO), Institut National de l'Environnement Industriel et des Risques (INERIS)-Université de Reims Champagne-Ardenne (URCA)-Université Le Havre Normandie (ULH), Normandie Université (NU)-Normandie Université (NU)-SFR Condorcet, Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS)-Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS), and Civs, Gestionnaire
Subjects: fish, diclofenac, LC-HRMS, [SDV.TOX.ECO] Life Sciences [q-bio]/Toxicology/Ecotoxicology, [SDV.TOX.ECO]Life Sciences [q-bio]/Toxicology/Ecotoxicology, metabolomics, Three-spined stickleback
Abstract: International audience; Introduction The aquatic systems are the ultimate repository of a large part of the compounds due to human activities. In response to these chemical stresses which aquatic environment may suffer, most studies have focused on the use of biomarkers as early responses induced by these pressures. In recent years, several studies showed that metabolomics can be used to compare and determine the causal link between exposure and effects for predicting toxic effects of contaminants in aquatic organisms. Especially, untargeted approaches based on high resolution mass spectrometry has gained more and more popularity due to its benefit of analysing the whole metabolome in a global view. This approach is a promising tool to provide biomarkers of effect and information on exposure ways in organisms in view of aquatic biomonitoring and environmental risk assessment. The aims of this study were : - To develop an exhaustive untargeted approach able to cover a huge range of metabolites within three-spined sticklebacks (Gasterosteus aculeatus) - To identify impacted metabolites after a sub-chronic exposure of this species to diclofenac. Materials and methods A solid liquid extraction followed by liquid chromatography (RSLC U3000 from Thermo Scientific®) coupled to QtoF (Maxis Plus from Bruker Daltonics®) were used. The data were processed using Metaboscape 4.0 (Bruker Daltonics®). The extraction process was performed on 25 or 50 mg of whole fish, liver, gills or brain with 2.5 mL of MeOH/H2O/heptane. Two complementary analytical methods were then combined : a reverse phase separation on HSS T3 C18 column for positive ionization mode, and an HILIC separation on Nucleodur column for negative ionization mode. This development was done to identify finer and then more relevant biomarkers by a comparative strategy between control and exposed individuals to diclofenac. Results and discussion Two LC-HRMS methods have been developed based on the responses of analytical standards representative of different metabolite groups (amino acids, sugars...). The extraction protocol has been optimized in all matrices, taking into account the number of features (m/z, tr) observed in LC-HRMS. The study shows the influence of the nature of the solvents during the extraction stage as well as the choice of chromatographic conditions. These methods were applied to adult fish exposed to diclofenac, a non-steroidal anti-inflammatory drug ubiquitous in the environment. Individuals were exposed for 21 days at 1 and 100 µg/L. The results of the untargeted metabolomic approach will be presented during the speech. Conclusions This study provides a complete reliable analytical method for the characterization of metabolites in three-spined sticklebacks. Thus, it enables to identify biomarkers through a comparative strategy, for biomonitoring of water bodies quality.
Published: 2021

27. Sex disparities in COVID-19 outcomes of inpatients with diabetes: insights from the CORONADO study

Author: L Bordier, Pierre Fontaine, Sandrine Lablanche, Abdallah Al-Salameh, Sarra Smati, Amandine Galioot, Adèle Lasbleiz, Olivier Bourron, Matthieu Wargny, Sandrine Coudol, Béatrice Guyomarch, Dominique Seret-Bégué, Sara Barraud, Eric Renard, Céline Gonfroy-Leymarie, Anna Flaus-Furmaniuk, Sybil Charrière, Bertrand Cariou, Emmanuel Cosson, Philippe Thuillier, Franck Mauvais-Jarvis, Jean-François Gautier, Bruno Vergès, Edith Bigot, Bruno Guerci, Bruno Fève, Matthieu Pichelin, Jean-Daniel Lalau, Louis Potier, Muriel Bourgeon, Samy Hadjadj, Gaëtan Prévost, Etienne Larger, Marion Munch, Nicolas Chevalier, Yves Reznik, Sophie Borot, Paul Sibilia, Pierre Gourdy, Bruno Laviolle, Coralie Amadou, Michel Marre, Blandine Tramunt, Institut des Maladies Métaboliques et Cardiovasculaires (I2MC), Université Toulouse III - Paul Sabatier (UT3), Université de Toulouse (UT)-Université de Toulouse (UT)-Institut National de la Santé et de la Recherche Médicale (INSERM), Unité de recherche de l'institut du thorax (ITX-lab), Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Université de Nantes - UFR de Médecine et des Techniques Médicales (UFR MEDECINE), Université de Nantes (UN)-Université de Nantes (UN), Centre hospitalier universitaire de Nantes (CHU Nantes), CHU Amiens-Picardie, Périnatalité et Risques Toxiques - UMR INERIS_I 1 (PERITOX), Institut National de l'Environnement Industriel et des Risques (INERIS)-Université de Picardie Jules Verne (UPJV)-CHU Amiens-Picardie, Centre Hospitalier Sud Francilien, Centre Hospitalier Universitaire de Reims (CHU Reims), Centre de Recherche en Sciences et Technologies de l'Information et de la Communication - EA 3804 (CRESTIC), Université de Reims Champagne-Ardenne (URCA), Hôpital d'Instruction des Armées Begin, Service de Santé des Armées, Service de Diabétologie - Endocrinologie [CHRU Besançon], Centre Hospitalier Régional Universitaire de Besançon (CHRU Besançon), AP-HP - Hôpital Antoine Béclère [Clamart], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), AP-HP Hôpital Bicêtre (Le Kremlin-Bicêtre), CHU Pitié-Salpêtrière [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU), Centre de Recherche des Cordeliers (CRC (UMR_S_1138 / U1138)), École Pratique des Hautes Études (EPHE), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU)-Université Paris Cité (UPCité), Institut de Cardiométabolisme et Nutrition = Institute of Cardiometabolism and Nutrition [CHU Pitié Salpêtrière] (IHU ICAN), Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU), Hôpital Louis Pradel [CHU - HCL], Hospices Civils de Lyon (HCL), Cardiovasculaire, métabolisme, diabétologie et nutrition (CarMeN), Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Hospices Civils de Lyon (HCL)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Centre Hospitalier Universitaire de Nice (CHU Nice), Hôpital Jean Verdier [AP-HP], Centre de Recherche Épidémiologie et Statistique Sorbonne Paris Cité (CRESS (U1153 / UMR_A_1125 / UMR_S_1153)), Conservatoire National des Arts et Métiers [CNAM] (CNAM), HESAM Université - Communauté d'universités et d'établissements Hautes écoles Sorbonne Arts et métiers université (HESAM)-HESAM Université - Communauté d'universités et d'établissements Hautes écoles Sorbonne Arts et métiers université (HESAM)-Université Sorbonne Paris Cité (USPC)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Paris Cité (UPCité)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), CHU Saint-Antoine [AP-HP], Centre hospitalier Félix-Guyon [Saint-Denis, La Réunion], Centre Hospitalier Régional Universitaire [Lille] (CHRU Lille), Hôpital Claude Huriez [Lille], CHU Lille, CHU Bordeaux [Bordeaux], Centre Hospitalier René Dubos [Pontoise], Centre Hospitalier Régional Universitaire de Nancy (CHRU Nancy), CHU Grenoble, AP-HP - Hôpital Cochin Broca Hôtel Dieu [Paris], Assistance Publique - Hôpitaux de Marseille (APHM), Centre recherche en CardioVasculaire et Nutrition = Center for CardioVascular and Nutrition research (C2VN), Aix Marseille Université (AMU)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Centre d'Investigation Clinique [Rennes] (CIC), Université de Rennes (UR)-Hôpital Pontchaillou-Institut National de la Santé et de la Recherche Médicale (INSERM), CHU Pontchaillou [Rennes], Hôpital Ambroise Paré [AP-HP], CHU Strasbourg, AP-HP - Hôpital Bichat - Claude Bernard [Paris], Différenciation et communication neuronale et neuroendocrine (DC2N), Université de Rouen Normandie (UNIROUEN), Normandie Université (NU)-Normandie Université (NU)-Institut National de la Santé et de la Recherche Médicale (INSERM), Service d'endocrinologie, diabétologie et maladies métaboliques [Rouen], CHU Rouen, Normandie Université (NU)-Normandie Université (NU)-Université de Rouen Normandie (UNIROUEN), Normandie Université (NU), CHU Montpellier, Centre Hospitalier Régional Universitaire [Montpellier] (CHRU Montpellier), Institut de Génomique Fonctionnelle (IGF), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS), CHU Caen, Normandie Université (NU)-Tumorothèque de Caen Basse-Normandie (TCBN), Centre Hospitalier de Gonesse, Centre Hospitalier Universitaire d'Angers (CHU Angers), PRES Université Nantes Angers Le Mans (UNAM), Groupe d'Etude de la Thrombose de Bretagne Occidentale (GETBO), Université de Brest (UBO)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut Brestois Santé Agro Matière (IBSAM), Université de Brest (UBO)-Université de Brest (UBO), CHU Dijon, Centre Hospitalier Universitaire de Dijon - Hôpital François Mitterrand (CHU Dijon), Service de Diabétologie et Endocrinologie, Centre d’Investigation Clinique de Nantes (CIC Nantes), Université de Nantes (UN)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre hospitalier universitaire de Nantes (CHU Nantes), Tulane University, Centre Hospitalier Universitaire de Toulouse (CHU Toulouse), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National de la Santé et de la Recherche Médicale (INSERM), Service de Diabétologie [CHU Pitié-Salpétrière], École pratique des hautes études (EPHE), CRHU Nancy, Jonchère, Laurent, Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées, unité de recherche de l'institut du thorax UMR1087 UMR6291 (ITX), Université de Picardie Jules Verne (UPJV)-CHU Amiens-Picardie-Institut National de l'Environnement Industriel et des Risques, CH Evry-Corbeil, Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-Hôpital Pontchaillou-Institut National de la Santé et de la Recherche Médicale (INSERM), Université de Montpellier (UM)-Université Montpellier 1 (UM1)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Montpellier 2 - Sciences et Techniques (UM2)-Centre National de la Recherche Scientifique (CNRS), Institut Brestois Santé Agro Matière (IBSAM), Université de Brest (UBO)-Université de Brest (UBO)-Université de Brest (UBO), and CHU Toulouse [Toulouse]
Subjects: Male, Endocrinology, Diabetes and Metabolism, [SDV]Life Sciences [q-bio], 030204 cardiovascular system & hematology, Severity of Illness Index, law.invention, 0302 clinical medicine, Endocrinology, Risk Factors, law, Hospital Mortality, Aged, 80 and over, [SDV.MHEP.EM] Life Sciences [q-bio]/Human health and pathology/Endocrinology and metabolism, Sex Characteristics, COPD, Incidence, Incidence (epidemiology), General Medicine, Middle Aged, [SDV.MHEP.EM]Life Sciences [q-bio]/Human health and pathology/Endocrinology and metabolism, Prognosis, Intensive care unit, 3. Good health, Hospitalization, [SDV] Life Sciences [q-bio], Intensive Care Units, Population study, Female, France, Sex characteristics, medicine.medical_specialty, Diabetes Complications, 03 medical and health sciences, Internal medicine, Diabetes mellitus, Severity of illness, Diabetes Mellitus, medicine, Humans, Aged, Retrospective Studies, Inpatients, SARS-CoV-2, business.industry, COVID-19, Retrospective cohort study, medicine.disease, Respiration, Artificial, business, 030217 neurology & neurosurgery
Abstract: Objective Male sex is one of the determinants of severe coronavirus diseas-e-2019 (COVID-19). We aimed to characterize sex differences in severe outcomes in adults with diabetes hospitalized for COVID-19. Methods We performed a sex-stratified analysis of clinical and biological features and outcomes (i.e. invasive mechanical ventilation (IMV), death, intensive care unit (ICU) admission and home discharge at day 7 (D7) or day 28 (D28)) in 2380 patients with diabetes hospitalized for COVID-19 and included in the nationwide CORONADO observational study (NCT04324736). Results The study population was predominantly male (63.5%). After multiple adjustments, female sex was negatively associated with the primary outcome (IMV and/or death, OR: 0.66 (0.49–0.88)), death (OR: 0.49 (0.30–0.79)) and ICU admission (OR: 0.57 (0.43–0.77)) at D7 but only with ICU admission (OR: 0.58 (0.43–0.77)) at D28. Older age and a history of microvascular complications were predictors of death at D28 in both sexes, while chronic obstructive pulmonary disease (COPD) was predictive of death in women only. At admission, C-reactive protein (CRP), aspartate amino transferase (AST) and estimated glomerular filtration rate (eGFR), according to the CKD-EPI formula predicted death in both sexes. Lymphocytopenia was an independent predictor of death in women only, while thrombocytopenia and elevated plasma glucose concentration were predictors of death in men only. Conclusions In patients with diabetes admitted for COVID-19, female sex was associated with lower incidence of early severe outcomes, but did not influence the overall in-hospital mortality, suggesting that diabetes mitigates the female protection from COVID-19 severity. Sex-associated biological determinants may be useful to optimize COVID-19 prevention and management in women and men.
Published: 2021

28. Low‐Level Radiofrequency Exposure Induces Vasoconstriction in Rats

Author: René de Seze, Anne Braun, Véronique Bach, Thi Cuc Mai, Amandine Pelletier, Institut National de l'Environnement Industriel et des Risques (INERIS), Périnatalité et Risques Toxiques - UMR INERIS_I 1 (PERITOX), and Université de Picardie Jules Verne (UPJV)-CHU Amiens-Picardie-Institut National de l'Environnement Industriel et des Risques
Subjects: medicine.medical_specialty, Radio Waves, Physiology, Rf exposure, medicine.medical_treatment, [SDV]Life Sciences [q-bio], Intraperitoneal injection, ambient temperature, Biophysics, Cold exposure, Vasodilation, 02 engineering and technology, Body Temperature, 030218 nuclear medicine & medical imaging, 03 medical and health sciences, chemistry.chemical_compound, 0302 clinical medicine, radiofrequency, Internal medicine, 0202 electrical engineering, electronic engineering, information engineering, medicine, Animals, vasoconstriction, Radiology, Nuclear Medicine and imaging, Neurotransmitter, Antagonist, 020206 networking & telecommunications, General Medicine, tail skin temperature, thermogenesis, Rats, Endocrinology, chemistry, medicine.symptom, Skin Temperature, Thermogenesis, Vasoconstriction, Body Temperature Regulation
Abstract: Recent studies have revealed that rodents' physiological responses to low-intensity radiofrequency (RF) electromagnetic fields were similar to thermoregulatory responses to cold conditions. The primary autonomic response to cold exposure is peripheral vasoconstriction that allows rodents to reduce heat loss and maintain a relatively constant internal body temperature. In the present study, we investigated the effects of 900 MHz RF at a low level (SAR of 0.35 W/kg) on tail skin temperature (Ttail ) in rats. We showed that rats exposed to RF had lower Ttail than control rats at ambient temperatures between 27 and 28 °C, suggesting that RF could induce a noticeable degree of vasoconstriction under mild-warm ambient temperatures. This difference in Ttail was suppressed after the intraperitoneal injection of a vasodilator, an α-adrenergic antagonist, confirming the hypothesis of the vasoconstriction in exposed rats. Moreover, like a response to cold stimuli, RF exposure led to increased plasma concentrations of important factors: noradrenaline (a neurotransmitter responsible for vasoconstriction and thermogenesis) and fatty acids (markers of activated thermogenesis). Taken together, these findings indicate that low-intensity RF levels triggered some key physiological events usually associated with responses to cold in rats. © 2021 Bioelectromagnetics Society.
Published: 2021

29. The Utility of Dreissena polymorpha for Assessing the Viral Contamination of Rivers by Measuring the Accumulation of F-Specific RNA Bacteriophages

Author: Isabelle Bonnard, Christophe Gantzer, Mélissa Palos Ladeiro, Alain Geffard, Nicolas Boudaud, Sandrine Capizzi-Banas, Fanny Bastien, Laboratoire de Chimie Physique et Microbiologie pour les Matériaux et l'Environnement (LCPME), Université de Lorraine (UL)-Centre National de la Recherche Scientifique (CNRS), Stress Environnementaux et BIOsurveillance des milieux aquatiques (SEBIO), Université Le Havre Normandie (ULH), Normandie Université (NU)-Normandie Université (NU)-Université de Reims Champagne-Ardenne (URCA)-SFR Condorcet, Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS)-Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS)-Institut National de l'Environnement Industriel et des Risques (INERIS), Actalia [Saint-Lô], Institut de Chimie du CNRS (INC)-Université de Lorraine (UL)-Centre National de la Recherche Scientifique (CNRS), Institut National de l'Environnement Industriel et des Risques (INERIS)-Université de Reims Champagne-Ardenne (URCA)-Université Le Havre Normandie (ULH), Normandie Université (NU)-Normandie Université (NU)-SFR Condorcet, and Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS)-Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS)
Subjects: Pollution, lcsh:Hydraulic engineering, animal structures, media_common.quotation_subject, [SDV]Life Sciences [q-bio], Geography, Planning and Development, 010501 environmental sciences, Aquatic Science, 01 natural sciences, Biochemistry, Dreissena, 03 medical and health sciences, lcsh:Water supply for domestic and industrial purposes, bacteriophage, lcsh:TC1-978, 14. Life underwater, 0105 earth and related environmental sciences, Water Science and Technology, media_common, lcsh:TD201-500, 0303 health sciences, biology, 030306 microbiology, surface water, mollusk, Contamination, biology.organism_classification, 6. Clean water, bioaccumulation, Wastewater, 13. Climate action, Bioaccumulation, Environmental chemistry, biomonitoring, [SDV.MP.VIR]Life Sciences [q-bio]/Microbiology and Parasitology/Virology, Zebra mussel, Viral load, Surface water
Abstract: River water that receives treated wastewater can be contaminated by pathogens including enteric viruses due to fecal pollution, which may represent an important public health hazard. There is a great diversity of enteric viruses and fecal bacteriophages, especially F-specific RNA bacteriophages (FRNAPHs), are commonly proposed as indicators of viral pollution due to a variety of characteristics such as their structural similarities to the main enteric viruses, their high concentrations in raw wastewater and their environmental survival rate, which is better than other cultivable enteric viruses. However, evaluating the viral contamination of water on the basis of FRNAPH concentration levels continues to present a challenge. This is because the quality of detection is strongly dependent on the quantity of viral particles, high spatio-temporal variabilities and the physico-chemical conditions of the water during sampling. To overcome these limitations, the present study aims to evaluate whether the bivalve mollusk Dreissena polymorpha (zebra mussel) could be considered a suitable experimental model for assessing the viral contamination of rivers. In order to determine this, the capacity of D. polymorpha to accumulate FRNAPHs and assimilate them into their soft tissue was studied. This provided a proof of concept for the use of D. polymorpha to evaluate the viral contamination of surface water. Two experiments were conducted: (1) an in situ experiment to confirm that zebra mussels naturally accumulated FRNAPHs and (2) a laboratory experiment to determine the accumulation and depuration kinetics of FRNAPHs in D. polymorpha tissue. The study highlights the capacity of the mussels to accumulate infectious FRNAPHs both on a laboratory scale under controlled conditions as well as in situ at different sites that are representative of different bodies of water. An analysis of the mussels’ soft tissue showed that they were capable of reflecting the water’s contamination level very quickly (within less than 24 h). Moreover, the soft tissue retained the viral load much longer than the water due to a low depuration rate. The analysis of FRNAPH concentrations in mussels exposed in situ suggested that there were differences in contamination levels between sites. These preliminary results underline the potential utility of zebra mussels in assessing viral contamination by measuring the accumulation of FRNAPHs in their tissue. This may ultimately enable stakeholders to use zebra mussels as a means of monitoring viral pollution in surface water.
Published: 2021

30. Effects of diclofenac on sentinel species and aquatic communities in semi-natural conditions

Author: Sandrine Joachim, Odile Dedourge-Geffard, Emmanuelle Vulliet, Rémy Beaudouin, Jean-Marc Porcher, Mélissa Palos-Ladeiro, A. James, Anne Bado-Nilles, Cleo Tebby, Sandrine Andres, Viviane David, Patrick Baudoin, Olivier Palluel, Adeline Geffard, Maéva Fieu, Gaëlle Daniele, Cyril Turies, Marc Bonnard, Stress Environnementaux et BIOsurveillance des milieux aquatiques (SEBIO), Institut National de l'Environnement Industriel et des Risques (INERIS)-Université de Reims Champagne-Ardenne (URCA)-Université Le Havre Normandie (ULH), Normandie Université (NU)-Normandie Université (NU)-SFR Condorcet, Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS)-Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS), Expertise en toxicologie/écotoxicologie des substances chimiques (ETES), Institut National de l'Environnement Industriel et des Risques (INERIS), TRACES - Technologie et Recherche en Analyse Chimique pour l'Environnement et la Santé, Institut des Sciences Analytiques (ISA), Institut de Chimie du CNRS (INC)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS), Unit of Models for Ecotoxicology and Toxicology (METO), and This study was funded by the French Ministry in Charge of Ecology and Sustainable Development within the frameworks of the Programme 190 and by the project 'DOREMIPHARM' supported by the French National Agency for Medicines and Health Products Safety.
Subjects: Aquatic Organisms, Diclofenac, Health, Toxicology and Mutagenesis, Sentinel species, 0211 other engineering and technologies, Fresh Water, 02 engineering and technology, 010501 environmental sciences, 01 natural sciences, Dreissena, Zooplankton, Environmental pollution, Mesocosm, Animal science, Abundance (ecology), [CHIM.ANAL]Chemical Sciences/Analytical chemistry, Animals, GE1-350, 14. Life underwater, 0105 earth and related environmental sciences, Invertebrate, 021110 strategic, defence & security studies, Primary producers, biology, Fishes, Public Health, Environmental and Occupational Health, General Medicine, biology.organism_classification, Pollution, Smegmamorpha, 6. Clean water, Macrophyte, Environmental sciences, Fish, Zebra mussels, TD172-193.5, Sentinel Species, [SDE]Environmental Sciences, Water Pollutants, Chemical
Abstract: Due to the potential hazard of diclofenac on aquatic organisms and the lack of higher-tier ecotoxicological studies, a long-term freshwater mesocosm experiment was set up to study the effects of this substance on primary producers and consumers at environmentally realistic nominal concentrations 0.1, 1 and 10 µg/L (average effective concentrations 0.041, 0.44 and 3.82 µg/L). During the six-month exposure period, the biovolume of two macrophyte species (Nasturtium officinale and Callitriche platycarpa) significantly decreased at the highest treatment level. Subsequently, a decrease in dissolved oxygen levels was observed. High mortality rates, effects on immunity, and high genotoxicity were found for encaged zebra mussels (Dreissena polymorpha) in all treatments. In the highest treatment level, one month after the beginning of the exposure, mortality of adult fish (Gasterosteus aculeatus) caused effects on the final population structure. Total abundance of fish and the percentage of juveniles decreased whereas the percentage of adults increased. This led to an overall shift in the length frequency distribution of the F1 generation compared to the control. Consequently, indirect effects on the community structure of zooplankton and macroinvertebrates were observed in the highest treatment level. The No Observed Effect Concentration (NOEC) value at the individual level was
Published: 2021

31. Impact of abdominal or pelvic radiotherapy on disease activity in inflammatory bowel disease: a multicentre cohort study from the GETAID

Author: Broussard, Doriane, Riviere, Pauline, Bonnet, Joelle, Fotsing, Ginette, Amiot, Aurelien, Peyrin-Biroulet, Laurent, Rajca, Sylvie, Buisson, Anthony, Gilleta, Cyrielle, Pelletier, Anne-Laure, Serrero, Melanie, Bouguen, Guillaume, Altwegg, Romain, Hebuterne, Xavier, Nancey, Stephane, Fumery, Mathurin, Cadiot, Guillaume, Nahon, Stephane, Rahier, Jean-Francois, Gornet, Jean-Marc, Vendrely, Véronique, Laharie, David, CHU Bordeaux [Bordeaux], Centre Hospitalier Universitaire de Nancy (CHU Nancy), CHU Toulouse [Toulouse], CHU Marseille, CIC - Biotherapie - Marseille, Institut National de la Santé et de la Recherche Médicale (INSERM), Hôpital Nord [CHU - APHM], Nutrition, Métabolismes et Cancer (NuMeCan), Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES), CHU Pontchaillou [Rennes], CHU Amiens-Picardie, Périnatalité et Risques Toxiques - UMR INERIS_I 1 (PERITOX), Université de Picardie Jules Verne (UPJV)-CHU Amiens-Picardie-Institut National de l'Environnement Industriel et des Risques, Centre Hospitalier Universitaire de Reims (CHU Reims), Groupe Hospitalier Intercommunal Le Raincy-Montfermeil, Centre Hospitalier Universitaire de Toulouse (CHU Toulouse), Université de Rennes (UR)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), and Institut National de l'Environnement Industriel et des Risques (INERIS)-Université de Picardie Jules Verne (UPJV)-CHU Amiens-Picardie
Subjects: Cohort Studies, Crohn Disease, [SDV]Life Sciences [q-bio], Abdomen, Humans, Female, Middle Aged, Inflammatory Bowel Diseases, Retrospective Studies
Abstract: International audience; Background - Abdominal or pelvic radiotherapy in inflammatory bowel disease (IBD) patients raises concerns regarding the risk of worsening of underlying disease. Aim - To assess the impact of radiotherapy on IBD course. Methods - A retrospective multicentre study including IBD patients exposed to abdominal or pelvic irradiation was conducted, retrieving IBD activity by semester (6-month periods) before (from S-4 to S-1) and after (from S + 1 to S + 6) radiotherapy and IBD flare during follow-up. Results - Sixty-one patients (32 women, mean age 59 years), with 467 patient semesters of follow-up, treated for digestive (n = 31), urinary tract (n = 23) and gynaecological cancers (n = 7) were included. Rates of IBD activity per semester were, respectively, 21% (95% CI: 16-27) from S-4 to S-1; 12% (7-19) from S + 1 to S + 3 (P = 0.15 vs S-4 to S-1) and 16% (10-25) from S + 4 to S + 6 (P = 0.45 vs S-4 to S-1). With a median follow-up of 156 weeks (interquartile range: 82-365), rates of survival without IBD flare at 1 and 3 years after radiotherapy were 82.5% (73.2-93.0) and 70.6% (58.8-84.7). Moderate-to-severe acute radiotherapy-induced gut toxicity and the absence of concomitant chemotherapy were independently associated with an increased risk of flare. Conclusion - Most patients with non-active IBD can be safely treated with abdominal or pelvic radiotherapy. Patients having acute gut toxicity and those without concomitant chemotherapy should be more closely monitored in the post-radiotherapy period.
Published: 2021

32. Multistate Models of Developmental Toxicity: Application to Valproic Acid-Induced Malformations in the Zebrafish Embryo

Author: Rémy Beaudouin, Frédéric Y. Bois, Thomas Braunbeck, Katharina Brotzmann, Ségolène Siméon, Institut National de l'Environnement Industriel et des Risques (INERIS), Stress Environnementaux et BIOsurveillance des milieux aquatiques (SEBIO), Université Le Havre Normandie (ULH), Normandie Université (NU)-Normandie Université (NU)-Université de Reims Champagne-Ardenne (URCA)-SFR Condorcet, Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS)-Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS)-Institut National de l'Environnement Industriel et des Risques (INERIS), Centre for Organismal Studies [Heidelberg] (COS), Heidelberg University, and Certara UK Limited
Subjects: 0301 basic medicine, Embryo, Nonmammalian, animal structures, Multistate model, Developmental toxicity, Toxicology, Models, Biological, Andrology, 03 medical and health sciences, 0302 clinical medicine, Toxicity Tests, Acute, Animals, Computer Simulation, Zebrafish, Survival analysis, Pharmacology, [STAT.AP]Statistics [stat]/Applications [stat.AP], biology, Dose-Response Relationship, Drug, Hatching, Valproic Acid, Abnormalities, Drug-Induced, Embryo, Bayes Theorem, Numerical Analysis, Computer-Assisted, biology.organism_classification, Acute toxicity, Toxicokinetics, 030104 developmental biology, Teratogens, 030220 oncology & carcinogenesis, Dose-response analysis, Toxicity, embryonic structures, Piecewise, Malformations, [SDV.TOX.ECO]Life Sciences [q-bio]/Toxicology/Ecotoxicology, Zebrafish embryo
Abstract: For the determination of acute toxicity of chemicals in zebrafish (Danio rerio) embryos, the OECD test guideline 236, relative to the Fish Embryo Toxicity Test (FET), stipulates a dose-response analysis of four lethal core endpoints and a quantitative characterization of abnormalities including their time-dependency. Routinely, the data are analysed at the different observation times separately. However, observations at a given time strongly depend on the previous effects and should be analysed jointly with them. To solve this problem, we developed multistate models for occurrence of developmental malformations and live events in zebrafish embryos exposed to eight concentrations of valproic acid (VPA) the first five days of life. Observations were recorded daily per embryo. We statistically infer on model structure and parameters using a numerical Bayesian framework. Hatching probability rate changed with time and we compared five forms of its time-dependence; a constant rate, a piecewise constant rate with a fixed hatching time at 48 hours post fertilization, a piecewise constant rate with a variable hatching time, as well as a Hill and Gaussian form. A piecewise constant function of time adequately described the hatching data. The other transition rates were conditioned on the embryo body concentration of VPA, obtained using a physiologically-based pharmacokinetic model. VPA impacted mostly the malformation probability rate in hatched and non-hatched embryos. Malformation reversion probability rates were lowered by VPA. Direct mortality was low at the concentrations tested, but increased linearly with internal concentration. The model makes full use of data and gives a finer grain analysis of the teratogenic effects of VPA in zebrafish than the OECD-prescribed approach. We discuss the use of the model for obtaining toxicological reference values suitable for inter-species extrapolation. A general result is that complex multistate models can be efficiently evaluated numerically.
Published: 2021
Full Text: View/download PDF

33. Genetic analyses of a large cohort of infertile patients with globozoospermia, DPY19L2 still the main actor, GGN confirmed as a guest player

Author: Ségolène Veau, Gilles Schuler, Aline Papaxanthos, Julien Bessonnat, Marine Poulain, H. Lejeune, Laurent Pasquier, Rosalie Cabry, Raoudha Zouari, Amandine Septier, Flore Mietton, Myriam Chaabouni, Vincent Achard, Zine-Eddine Kherraf, Charles Coutton, Antoine Clergeau, Dominique Lauton, Christophe Sifer, Béatrice Dorphin, Catherine Guillemain, Mahmoud Kharouf, Tristan Celse, Sebti Benbouhadja, Nathalie Sermondade, Selima Fourati Ben Mustapha, Pierre F. Ray, Delphine Martinez, Bernard Foliguet, Julie Beurois, Valérie Mitchell, Guillaume Martinez, Abdelali Zoghmar, Véronique Satre, Ahmed Chargui, Gérard Tachdjian, Cynthia Frapsauce, Chema Triki, Sylviane Hennebicq, Jacques Puechberty, Caroline Cazin, Christophe Arnoult, Nicolas Thierry-Mieg, Lionel Mery, Institute for Advanced Biosciences / Institut pour l'Avancée des Biosciences (Grenoble) (IAB), Centre Hospitalier Universitaire [Grenoble] (CHU)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Etablissement français du sang - Auvergne-Rhône-Alpes (EFS)-Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes (UGA), Centre Hospitalier Universitaire [Grenoble] (CHU), Biologie Computationnelle et Modélisation (TIMC-BCM ), Translational Innovation in Medicine and Complexity / Recherche Translationnelle et Innovation en Médecine et Complexité - UMR 5525 (TIMC ), VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes (UGA)-Institut polytechnique de Grenoble - Grenoble Institute of Technology (Grenoble INP ), Université Grenoble Alpes (UGA)-VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes (UGA)-Institut polytechnique de Grenoble - Grenoble Institute of Technology (Grenoble INP ), Université Grenoble Alpes (UGA), Marseille medical genetics - Centre de génétique médicale de Marseille (MMG), Institut National de la Santé et de la Recherche Médicale (INSERM)-Aix Marseille Université (AMU), Hôpital de la Conception [CHU - APHM] (LA CONCEPTION), Service Endocrinologie - Diabétologie [CHU Caen], Université de Caen Normandie (UNICAEN), Normandie Université (NU)-Normandie Université (NU)-CHU Caen, Normandie Université (NU)-Tumorothèque de Caen Basse-Normandie (TCBN)-Tumorothèque de Caen Basse-Normandie (TCBN), Polyclinique les Jasmins [Tunis], CHU Ibn Rochd [Casablanca], Service d'Histologie-Embryologie, Biologie de la Reproduction (CECOS Paris Cochin), Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Hôpital Cochin [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), CHU Bordeaux [Bordeaux], Laboratoire d’Aide Médicale à la Procréation [CH Alpes-Léman] (AMP 74), Centre AMP 74 [Contamine-sur-Arve]-Centre Hospitalier Alpes-Léman - CHAL, Institut Jean Lamour (IJL), Université de Lorraine (UL)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Hôpital Jean Verdier [AP-HP], Centre Hospitalier Régional Universitaire [Montpellier] (CHRU Montpellier), Stabilité génétique, Cellules Souches et Radiations (SCSR (U_967)), Université Paris-Sud - Paris 11 (UP11)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Paris (UP), Hospices Civils de Lyon (HCL), Hôpital Arnaud de Villeneuve [CHRU Montpellier], CHU Pontchaillou [Rennes], Centre Hospitalier Universitaire de Saint-Etienne (CHU de Saint-Etienne), Hôpital Foch [Suresnes], Gynécologie-obstétrique et médecine de la reproduction - Maternité [CHU Tenon], CHU Tenon [AP-HP], Sorbonne Université (SU)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), CHU Amiens-Picardie, Périnatalité et Risques Toxiques - UMR INERIS_I 1 (PERITOX), Université de Picardie Jules Verne (UPJV)-CHU Amiens-Picardie-Institut National de l'Environnement Industriel et des Risques, Hôpital Bretonneau, Centre Hospitalier Régional Universitaire de Tours (CHRU Tours), Assistance Publique - Hôpitaux de Marseille (APHM), Institut méditerranéen de biodiversité et d'écologie marine et continentale (IMBE), Avignon Université (AU)-Aix Marseille Université (AMU)-Institut de recherche pour le développement [IRD] : UMR237-Centre National de la Recherche Scientifique (CNRS), Institute for Advanced Biosciences, CR Inserm U1209, CNRS UMR5309, University Grenoble Alpes, Team « Génétique, Épigénétique et Thérapies de l'infertilité », Grenoble, France., CHU Grenoble Alpes, UM de GI-DPI, 38000 Grenoble, France, Biologie moléculaire et cellulaire de la différenciation, Université Joseph Fourier - Grenoble 1 (UJF)-Institut Albert Bonniot-Institut National de la Santé et de la Recherche Médicale (INSERM), Centre Hospitalier Universitaire de Grenoble, UM de Génétique Chromosomique, Biologie Computationnelle et Mathématique (TIMC-BCM ), Techniques de l'Ingénierie Médicale et de la Complexité - Informatique, Mathématiques et Applications, Grenoble - UMR 5525 (TIMC ), IMAG-VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes (UGA)-Institut polytechnique de Grenoble - Grenoble Institute of Technology (Grenoble INP ), Université Grenoble Alpes (UGA)-IMAG-VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes (UGA)-Institut polytechnique de Grenoble - Grenoble Institute of Technology (Grenoble INP ), Centre clinicobiologique d’AMP, pôle femmes–Parents-Enfants, hôpital de La Conception, AP–HM, 147, boulevard Baille, 13005 Marseille, France, Aix Marseille Université (AMU)-Institut National de la Santé et de la Recherche Médicale (INSERM), Physiologie de la reproduction et des comportements [Nouzilly] (PRC), Centre National de la Recherche Scientifique (CNRS)-Université de Tours-Institut Français du Cheval et de l'Equitation [Saumur]-Institut National de la Recherche Agronomique (INRA), CHU Cochin [AP-HP]-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), Department of Obstetrics and Gynecology, University Hospital Bordeaux, Bordeaux, France., Partenaires INRAE, Université de Lorraine (UL)-Centre National de la Recherche Scientifique (CNRS), University Hospital of Montpellier, Service d'histologie, embryologie et cytogénétique [Béclère], Université Paris-Sud - Paris 11 (UP11)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-AP-HP - Hôpital Antoine Béclère [Clamart], CH Sallanches, Service de Génétique [CHU Montpellier] (Hôpital Arnaud de Villeneuve), Hôpital Arnaud de Villeneuve-Centre Hospitalier Universitaire de Montpellier (CHU Montpellier ), Service de Génétique Clinique [CHU Rennes] (Réseau de Génétique et Génomique Médicale), Hôpitaux Universitaires du Grand Ouest, Service Gynécologie-Obstétrique et Médecine de la reproduction [Hôpital Tenon], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU), CHU Bretonneau, CHU Lille, Université de Lille, Centre National de la Recherche Scientifique (CNRS)-Institut de recherche pour le développement [IRD] : UMR237-Aix Marseille Université (AMU)-Avignon Université (AU), Institut de Chimie du CNRS (INC)-Université de Lorraine (UL)-Centre National de la Recherche Scientifique (CNRS), Université Paris-Sud - Paris 11 (UP11)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Paris Cité (UPCité), Centre Hospitalier Universitaire de Saint-Etienne [CHU Saint-Etienne] (CHU ST-E), Université de Versailles Saint-Quentin-en-Yvelines - UFR Sciences de la santé Simone Veil (UVSQ Santé), Université de Versailles Saint-Quentin-en-Yvelines (UVSQ), Institut National de l'Environnement Industriel et des Risques (INERIS)-Université de Picardie Jules Verne (UPJV)-CHU Amiens-Picardie, and Centre Hospitalier Alpes-Léman - CHAL -Centre AMP 74 [Contamine-sur-Arve]
Subjects: Male, Candidate gene, Biology, Polymorphism, Single Nucleotide, Cohort Studies, Teratozoospermia, 03 medical and health sciences, symbols.namesake, Exome Sequencing, Genetics, Humans, Genetic Testing, Acrosome, Gene, Genetic Association Studies, Infertility, Male, Genetics (clinical), Globozoospermia, Loss function, ComputingMilieux_MISCELLANEOUS, 030304 developmental biology, Sanger sequencing, 0303 health sciences, Homozygote, 030305 genetics & heredity, Membrane Proteins, [SDV.BDLR]Life Sciences [q-bio]/Reproductive Biology, Spermatozoa, Phenotype, Human genetics, 3. Good health, Testicular Hormones, [SDV.GEN.GH]Life Sciences [q-bio]/Genetics/Human genetics, Mutation, symbols, Gene Deletion
Abstract: International audience; Globozoospermia is a rare phenotype of primary male infertility inducing the production of round-headed spermatozoa without acrosome. Anomalies of DPY19L2 account for 50-70% of all cases and the entire deletion of the gene is by far the most frequent defect identified. Here, we present a large cohort of 69 patients with 20-100% of globozoospermia. Genetic analyses including multiplex ligation-dependent probe amplification, Sanger sequencing and whole-exome sequencing identified 25 subjects with a homozygous DPY19L2 deletion (36%) and 14 carrying other DPY19L2 defects (20%). Overall, 11 deleterious single-nucleotide variants were identified including eight novel and three already published mutations. Patients with a higher rate of round-headed spermatozoa were more often diagnosed and had a higher proportion of loss of function anomalies, highlighting a good genotype phenotype correlation. No gene defects were identified in patients carrying < 50% of globozoospermia while diagnosis efficiency rose to 77% for patients with > 50% of globozoospermia. In addition, results from whole-exome sequencing were scrutinized for 23 patients with a DPY19L2 negative diagnosis, searching for deleterious variants in the nine other genes described to be associated with globozoospermia in human (C2CD6, C7orf61, CCDC62, CCIN, DNAH17, GGN, PICK1, SPATA16, and ZPBP1). Only one homozygous novel truncating variant was identified in the GGN gene in one patient, confirming the association of GGN with globozoospermia. In view of these results, we propose a novel diagnostic strategy focusing on patients with at least 50% of globozoospermia and based on a classical qualitative PCR to detect DPY19L2 homozygous deletions. In the absence of the latter, we recommend to perform whole-exome sequencing to search for defects in DPY19L2 as well as in the other previously described candidate genes.
Published: 2021

34. Exposure of South Korean Population to 5G Mobile Phone Networks (3.4–3.8 GHz)

Author: Kihwea Kim, Pierre-Baptiste Petit, René de Seze, Brahim Selmaoui, Donggeun Choi, Paul Mazet, Institut National de l'Environnement Industriel et des Risques (INERIS), Périnatalité et Risques Toxiques - UMR INERIS_I 1 (PERITOX), Université de Picardie Jules Verne (UPJV)-CHU Amiens-Picardie-Institut National de l'Environnement Industriel et des Risques, CEntre Technique des Industries Mécaniques (CETIM), CEntre Technique des Industries Mécaniques - Cetim (FRANCE), and National Radio Research Agency (RRA)
Subjects: 3.4–3.8 GHz, Physiology, Radio Waves, [SDV]Life Sciences [q-bio], Biophysics, 02 engineering and technology, 7. Clean energy, 030218 nuclear medicine & medical imaging, 03 medical and health sciences, 0302 clinical medicine, Electromagnetic Fields, electromagnetic field, South Korea, Republic of Korea, 0202 electrical engineering, electronic engineering, information engineering, Radiology, Nuclear Medicine and imaging, business.industry, Korean population, 020206 networking & telecommunications, General Medicine, Environmental Exposure, Geography, Mobile phone, Radiation protection, business, Telecommunications, 5G, Cell Phone, radio frequencies
Abstract: As industrialized countries race to install and deploy 5G networks, some countries have taken the lead and already have operational 5G networks in place. South Korea is among these. In this study, we measured exposure to electromagnetic fields in South Korea to evaluate the relative contribution of 5G as compared with other frequencies such as 2G, 3G, and 4G. Results show that the emission of 5G contributes about 15% to total telecommunications emissions. The highest levels were observed in the vicinity of 5G antennas and remain below the limits set by the International Commission on Non-Ionizing Radiation Protection (ICNIRP). © 2021 Bioelectromagnetics Society.
Published: 2021

35. Water quality of the Meuse watershed: Assessment using a multi-biomarker approach with caged three-spined stickleback (Gasterosteus aculeatus L.)

Author: Audrey Catteau, Sébastien Ronkart, Cyril Turies, Cleo Tebby, Olivier Palluel, Alain Geffard, Nina Chrétien, Sandrine Joachim, Katherine Nott, Rémy Beaudouin, Anne Bado-Nilles, Jean-Marc Porcher, Institut National de l'Environnement Industriel et des Risques (INERIS), Stress Environnementaux et BIOsurveillance des milieux aquatiques (SEBIO), Université Le Havre Normandie (ULH), Normandie Université (NU)-Normandie Université (NU)-Université de Reims Champagne-Ardenne (URCA)-SFR Condorcet, and Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS)-Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS)-Institut National de l'Environnement Industriel et des Risques (INERIS)
Subjects: Fish Proteins, Three-spined stickleback, Health, Toxicology and Mutagenesis, 0211 other engineering and technologies, Zoology, 02 engineering and technology, Gasterosteus, 010501 environmental sciences, 01 natural sciences, Thiobarbituric Acid Reactive Substances, Antioxidants, Environmental pollution, Condition index, Vitellogenin, Vitellogenins, Caging, Rivers, Water Quality, Wastewater treatment plants, Biomonitoring, Animals, GE1-350, 0105 earth and related environmental sciences, 021110 strategic, defence & security studies, biology, Public Health, Environmental and Occupational Health, Stickleback, General Medicine, Biomarker, biology.organism_classification, Pollution, 6. Clean water, Smegmamorpha, Europe, Environmental sciences, Oxidative Stress, Fish, TD172-193.5, biology.protein, Biomarker (medicine), Water quality, France, [SDV.TOX.ECO]Life Sciences [q-bio]/Toxicology/Ecotoxicology, Biomarkers, Water Pollutants, Chemical, Environmental Monitoring
Abstract: The use of a multi-biomarker approach with three-spined sticklebacks (Gasterosteus aculeatus) through an active biomonitoring strategy appears to be a promising tool in water quality assessment. The present work proposes to assess the efficiency of these tools in the discrimination of some sites in a large scale on the Meuse basin in Europe. The study was part of an EU program which aims to assess water quality in the Meuse across the French-Belgian border. Sticklebacks were caged 21 days upstream and downstream from the wastewater treatment plants (WWTPs) of Namur (Belgium), Charleville-Mezieres (France), Bouillon (Belgium) and Avesnes-sur-Helpe (France). First, the state of a variety of physiological functions was assessed using a battery of biomarkers that represented innate immunity (leucocyte mortality and distribution, phagocytosis activity, respiratory burst), antioxidant system (GPx, CAT, SOD and total GSH content), oxidative damages to the membrane lipids (TBARS), biotransformation enzymes (EROD, GST), synaptic transmission (AChE) and reproduction system (spiggin and vitellogenin concentration). The impacts of the effluents were first analysed for each biomarker using a mixed model ANOVA followed by post-hoc analyses. Secondly, the global river contamination was assessed using a principal component analysis (PCA) followed by a hierarchical agglomerative clustering (HAC). The results highlighted a small number of effects of WWTP effluents on the physiological parameters in caged sticklebacks. Despite a significant effect of the “localisation” factor (upstream/downstream) in the mixed ANOVA for several biomarkers, post-hoc analyses revealed few differences between upstream and downstream of the WWTPs. Only a significant decrease of innate immune responses was observed downstream from the WWTPs of Avesnes-sur-Helpe and Namur. Other biomarker responses were not impacted by WWTP effluents. However, the multivariate analyses (PCA and HAC) of the biomarker responses helped to clearly discriminate the different study sites from the reference but also amongst themselves. Thus, a reduction of general condition (condition index and HSI) was observed in all groups of caged sticklebacks, associated with a weaker AChE activity in comparison with the reference population. A strong oxidative stress was highlighted in fish caged in the Meuse river at Charleville-Mezieres whereas sticklebacks caged in the Meuse river at Namur exhibited weaker innate immune responses than others. Conversely, sticklebacks caged in the Helpe-Majeure river at Avesnes-sur-Helpe exhibited higher immune responses. Furthermore, weak defence capacities were recorded in fish caged in the Semois river at Bouillon. This experiment was the first to propose an active biomonitoring approach using three-spined stickleback to assess such varied environments. Low mortality and encouraging results in site discrimination support the use of this tool to assess the quality of a large number of water bodies.
Published: 2021

36. Individual Exposure to Environmental Radiofrequency Electromagnetic Fields in Hospitalized Preterm Neonates

Author: René de Seze, Stéphane Delanaud, André Léké, Dimitri Besset, Erwan Stéphan-Blanchard, Brahim Selmaoui, Périnatalité et Risques Toxiques - UMR INERIS_I 1 (PERITOX), Université de Picardie Jules Verne (UPJV)-CHU Amiens-Picardie-Institut National de l'Environnement Industriel et des Risques, and Institut National de l'Environnement Industriel et des Risques (INERIS)
Subjects: Electromagnetic field, medicine.medical_specialty, Neonatal intensive care unit, Physiology, Radio Waves, [SDV]Life Sciences [q-bio], 030303 biophysics, Biophysics, 03 medical and health sciences, Electromagnetic Fields, newborn, Medicine, Humans, Radiology, Nuclear Medicine and imaging, ComputingMilieux_MISCELLANEOUS, 030304 developmental biology, 0303 health sciences, business.industry, Infant, Newborn, General Medicine, Environmental Exposure, neonatal intensive care unit, 3. Good health, radiofrequencies, Emergency medicine, exposure levels, business, environment
Abstract: International audience
Published: 2021

37. An optimized LC-HRMS untargeted metabolomics workflow for multi-matrices investigations in the three-spined stickleback

Author: Emmanuelle Lebeau-Roche, Gaëlle Daniele, Aurélie Fildier, Cyril Turies, Odile Dedourge-Geffard, Jean-Marc Porcher, Alain Geffard, Emmanuelle Vulliet, TRACES - Technologie et Recherche en Analyse Chimique pour l'Environnement et la Santé, Institut des Sciences Analytiques (ISA), Institut de Chimie du CNRS (INC)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS), Stress Environnementaux et BIOsurveillance des milieux aquatiques (SEBIO), Institut National de l'Environnement Industriel et des Risques (INERIS)-Université de Reims Champagne-Ardenne (URCA)-Université Le Havre Normandie (ULH), Normandie Université (NU)-Normandie Université (NU)-SFR Condorcet, Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS)-Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS), Institut National de l'Environnement Industriel et des Risques (INERIS), Université Le Havre Normandie (ULH), Normandie Université (NU)-Normandie Université (NU)-Université de Reims Champagne-Ardenne (URCA)-SFR Condorcet, and Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS)-Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS)-Institut National de l'Environnement Industriel et des Risques (INERIS)
Subjects: Gills, Male, Ionization, Respiratory System, RPLC, Biochemistry, Mass Spectrometry, Workflow, Metabolites, Medicine and Health Sciences, Animal Anatomy, HILIC, Gasterosteus aculeatus, ComputingMilieux_MISCELLANEOUS, Chemical Reactions, Eukaryota, Brain, Smegmamorpha, Chemistry, Liver, Osteichthyes, Physical Sciences, Vertebrates, [SDE]Environmental Sciences, Amino Acid Analysis, Metabolome, Medicine, Female, Anatomy, Research Article, Fish Biology, Science, Research and Analysis Methods, [CHIM.ANAL]Chemical Sciences/Analytical chemistry, Alkanes, Metabolomics, Animals, [CHIM]Chemical Sciences, Molecular Biology Techniques, Molecular Biology, three-spined stickleback, Sticklebacks, Molecular Biology Assays and Analysis Techniques, Organisms, Chemical Compounds, LC-QToF, Biology and Life Sciences, Hydrocarbons, untargeted metabolomics, Metabolism, Fish, Aquatic Respiratory Anatomy, Zoology, Chromatography, Liquid
Abstract: Environmental metabolomics has become a growing research field to understand biological and biochemical perturbations of organisms in response to various abiotic or biotic stresses. It focuses on the comprehensive and systematic analysis of a biologic system's metabolome. This allows the recognition of biochemical pathways impacted by a stressor, and the identification of some metabolites as biomarkers of potential perturbations occurring in a body. In this work, we describe the development and optimization of a complete reliable methodology based on liquid chromatography coupled to high resolution mass spectrometry (LC-HRMS) for untargeted metabolomics studies within a fish model species, the three-spined stickleback (Gasterosteus aculeatus). We evaluated the differences and also the complementarities between four different matrices (brain, gills, liver and whole fish) to obtain metabolome information. To this end, we optimized and compared sample preparation and the analytical method, since the type and number of metabolites detected in any matrix are closely related to these latter. For the sample preparation, a solid-liquid extraction was performed on a low quantity of whole fish, liver, brain, or gills tissues using combinations of methanol/water/heptane. Based on the numbers of features observed in LC-HRMS and on the responses of analytical standards representative of different metabolites groups (amino acids, sugars…), we discuss the influence of the nature, volume, and ratio of extraction solvents, the sample weight, and the reconstitution solvent. Moreover, the analytical conditions (LC columns, pH and additive of mobile phases and ionization modes) were also optimized so as to ensure the maximum metabolome coverages. Thus, two complementary chromatographic procedures were combined in order to cover a broader range of metabolites: a reversed phase separation (RPLC) on a C18 column followed by detection with positive ionization mode (ESI+) and a hydrophilic interaction chromatography (HILIC) on a zwitterionic column followed by detection with negative ionization mode (ESI-). This work provides information on brain, gills, liver, vs the whole body contribution to the stickleback metabolome. These information would help to guide ecotoxicological and biomonitoring studies.
Published: 2021

38. Effectiveness and safety of ustekinumab intensification at 90 Mg every four weeks In Crohn's disease: a multicenter study

Author: Mathurin, Fumery, Laurent, Peyrin-Biroulet, Stephane, Nancey, Romain, Altwegg, Cyrielle, Gilletta, Pauline, Veyrard, Guillaume, Bouguen, Stephanie, Viennot, Florian, Poullenot, Jerome, Filippi, Anthony, Buisson, Anne, Bozon, Franck, Brazier, Lieven, Pouillon, Bernard, Flourie, Lucile, Boivineau, Laurent, Siproudhis, David, Laharie, Xavier, Roblin, Momar, Diouf, Xavier, Treton, Jonchère, Laurent, Périnatalité et Risques Toxiques - UMR INERIS_I 1 (PERITOX), Institut National de l'Environnement Industriel et des Risques (INERIS)-Université de Picardie Jules Verne (UPJV)-CHU Amiens-Picardie, Service d'Hépato Gastroenterologie [CHU Amiens-Picardie], CHU Amiens-Picardie, Nutrition-Génétique et Exposition aux Risques Environnementaux (NGERE), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Lorraine (UL), Service d'Hépato-gastro-entérologie [CHRU Nancy], Centre Hospitalier Régional Universitaire de Nancy (CHRU Nancy), Autophagie infection et immunité - Autophagy Infection Immunity (APY), Centre International de Recherche en Infectiologie - UMR (CIRI), École normale supérieure - Lyon (ENS Lyon)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-École normale supérieure - Lyon (ENS Lyon)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Service d'Hépatogastroentérologie [CHU Lyon-Sud], Hospices Civils de Lyon (HCL), Département d'Hépato-Gastroentérologie et de Transplantation Hépatique [CHU Saint-Eloi], Hôpital Saint Eloi (CHRU Montpellier), Centre Hospitalier Régional Universitaire [Montpellier] (CHRU Montpellier)-Centre Hospitalier Régional Universitaire [Montpellier] (CHRU Montpellier)-Université de Montpellier (UM), Service de Gastroentérologie et pancréatologie [CHU Toulouse], Pôle Maladies de l'appareil digestif [CHU Toulouse], Centre Hospitalier Universitaire de Toulouse (CHU Toulouse)-Centre Hospitalier Universitaire de Toulouse (CHU Toulouse), Service de gastroentérologie [CHU Saint-Etienne], Centre Hospitalier Universitaire de Saint-Etienne (CHU de Saint-Etienne), Centre d'Investigation Clinique [Rennes] (CIC), Université de Rennes (UR)-Hôpital Pontchaillou-Institut National de la Santé et de la Recherche Médicale (INSERM), Nutrition, Métabolismes et Cancer (NuMeCan), Université de Rennes (UR)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Centre Hospitalier Universitaire [Rennes], Service d'Hépato-Gastro-Enterologie et Nutrition [CHU Caen], Université de Caen Normandie (UNICAEN), Normandie Université (NU)-Normandie Université (NU)-CHU Caen, Normandie Université (NU)-Tumorothèque de Caen Basse-Normandie (TCBN)-Tumorothèque de Caen Basse-Normandie (TCBN), Service d'Hépato-gastro-entérologie et oncologie digestive (CHU de Bordeaux), CHU Bordeaux [Bordeaux]-Hôpital Haut-Lévêque [CHU Bordeaux], CHU Bordeaux [Bordeaux], Hôpital Archet 2 [Nice] (CHU), Microbes, Intestin, Inflammation et Susceptibilité de l'Hôte (M2iSH), Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre de Recherche en Nutrition Humaine d'Auvergne (CRNH d'Auvergne)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Université Clermont Auvergne (UCA), Service d'Hépatologie Gastro-entérologie [CHU Clermont-Ferrand], CHU Estaing [Clermont-Ferrand], CHU Clermont-Ferrand-CHU Clermont-Ferrand, Service de Gastroentérologie [Hôpital Beaujon], Hôpital Beaujon [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), Service Gastroentérologie [CHU Bordeaux], Centre Hospitalier Universitaire de Bordeaux (CHU Bordeaux), Direction de la recherche clinique et de l'innovation [CHU Amiens], Université de Picardie Jules Verne (UPJV)-CHU Amiens-Picardie-Institut National de l'Environnement Industriel et des Risques, Institut National de la Santé et de la Recherche Médicale (INSERM)-École normale supérieure - Lyon (ENS Lyon)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-École normale supérieure - Lyon (ENS Lyon)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS), Université de Montpellier (UM)-CHU Saint-Eloi, Centre Hospitalier Universitaire Toulouse (CHU Toulouse), Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-Hôpital Pontchaillou-Institut National de la Santé et de la Recherche Médicale (INSERM), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Hôpital Haut-Lévêque [CHU Bordeaux], CHU Bordeaux [Bordeaux]-CHU Bordeaux [Bordeaux], Centre International de Recherche en Infectiologie (CIRI), École normale supérieure de Lyon (ENS de Lyon)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Université Jean Monnet - Saint-Étienne (UJM)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-École normale supérieure de Lyon (ENS de Lyon)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Université Jean Monnet - Saint-Étienne (UJM)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Service de Gastro-entérologie et Hépatologie [CHU Saint-Etienne], and Centre Hospitalier Universitaire de Saint-Etienne [CHU Saint-Etienne] (CHU ST-E)
Subjects: Crohn’s disease, [SDV] Life Sciences [q-bio], [SDV]Life Sciences [q-bio], [SDV.MHEP.HEG]Life Sciences [q-bio]/Human health and pathology/Hépatology and Gastroenterology, intensification, ustekinumab, [SDV.MHEP.HEG] Life Sciences [q-bio]/Human health and pathology/Hépatology and Gastroenterology
Abstract: International audience; Introduction: The approved maintenance regimens for ustekinumab in Crohn's disease (CD) are 90 mg every 8 or 12 weeks. Some patients will partially respond to ustekinumab or will experience a secondary loss of response. It remains poorly known if these patients may benefit from shortening the interval between injections.Methods: All patients with active CD, as defined by Harvey-Bradshaw score ≥ 4 and one objective sign of inflammation (CRP > 5 mg/L and/or fecal calprotectin > 250 µg/g and/or radiologic and/or endoscopic evidence of disease activity) who required ustekinumab dose escalation to 90mg every 4 weeks for loss of response or incomplete response to ustekinumab 90mg every 8 weeks were included in this retrospective multicenter cohort study.Results: One hundred patients, with a median age of 35 years (Interquartile Range (IQR), 28 - 49) and median disease duration of 12 (7 - 20) years were included. Dose intensification was performed after a median of 5.0 (2.8 - 9.0) months of ustekinumab treatment and was associated with corticosteroids and immunosuppressants in respectively 29% and 27% of cases. Short-term clinical response and clinical remission were observed in respectively 61% and 31% after a median of 2.4 (1.3 - 3.0) months. After a median follow-up of 8.2 (5.6-12.4) months, 61% of patients were still treated with ustekinumab, and 26% in steroid-free clinical remission. Among the 39 patients with colonoscopy during follow-up, 14 achieved endoscopic remission (no ulcers). At the end of follow-up, 27% of patients were hospitalized, and 19% underwent intestinal resection surgery. Adverse events were reported in 12% of patients, including five serious adverse events.Conclusion: In this multicenter study, two-thirds of patients recaptured response following treatment intensification with ustekinumab 90 mg every 4 weeks.
Published: 2021

39. Spatio-temporal assessment of pregnant women exposure to chlorpyrifos at a regional scale

Author: Frédéric Tognet, Laure Malherbe, Céline Brochot, Laurent Létinois, François Lestremau, Roseline Bonnard, Mohammed Guedda, Julien Caudeville, Véronique Bach, Karen Chardon, Florence Anna Zeman, Corentin Regrain, Emmanuelle Boulvert, Fabrice Marliere, Institut National de l'Environnement Industriel et des Risques (INERIS), Laboratoire Amiénois de Mathématique Fondamentale et Appliquée - UMR CNRS 7352 (LAMFA), Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS), Périnatalité et Risques Toxiques - UMR INERIS_I 1 (PERITOX), and Université de Picardie Jules Verne (UPJV)-CHU Amiens-Picardie-Institut National de l'Environnement Industriel et des Risques
Subjects: Physiologically based pharmacokinetic modelling, Insecticides, Epidemiology, 030501 epidemiology, Toxicology, Atmospheric sciences, 03 medical and health sciences, chemistry.chemical_compound, Pregnancy, Biomonitoring, Humans, ComputingMilieux_MISCELLANEOUS, Exposure assessment, 2. Zero hunger, Public Health, Environmental and Occupational Health, Agriculture, Environmental exposure, Environmental Exposure, Atmospheric dispersion modeling, Pollution, TCPy, chemistry, Chlorpyrifos, [SDE]Environmental Sciences, Environmental science, Female, Pregnant Women, 0305 other medical science, Scale (map), Biomarkers
Abstract: BACKGROUND The aim of this study was to use an integrated exposure assessment approach, combining spatiotemporal modeling of environmental exposure and fate of the chemical to assess the exposure of vulnerable populations. In this study, chlorpyrifos exposure of pregnant women in Picardy was evaluated at a regional scale during 1 year. This approach provided a mapping of exposure indicators of pregnant women to chlorpyrifos over fine spatial and temporal resolutions using a GIS environment. METHODS Fate and transport models (emission, atmospheric dispersion, multimedia exposure, PBPK) were combined with environmental databases in a GIS environment. Quantities spread over agricultural fields were simulated and integrated into a modeling chain coupling models. The fate and transport of chlorpyrifos was characterized by an atmospheric dispersion statistical metamodel and the dynamiCROP model. Then, the multimedia model Modul'ERS was used to predict chlorpyrifos daily exposure doses which were integrated in a PBPK model to compute biomarker of exposure (TCPy urinary concentrations). For the concentration predictions, two scenarios (lower bound and upper bound) were built. RESULTS At fine spatio-temporal resolutions, the cartography of biomarkers in the lower bound scenario clearly highlights agricultural areas. In these maps, some specific areas and hotspots appear as potentially more exposed specifically during application period. Overall, predictions were close to biomonitoring data and ingestion route was the main contributor to chlorpyrifos exposure. CONCLUSIONS This study demonstrated the feasibility of an integrated approach for the evaluation of chlorpyrifos exposure which allows the comparison between modeled predictions and biomonitoring data.
Published: 2021

40. Evolution of Endoscopic Lesions in Steroid-Refractory Acute Severe Ulcerative Colitis Responding to Infliximab or Cyclosporine

Author: Arnaud Bourreille, Julien Branche, Laurent Beaugerie, Yoram Bouhnik, Stéphane Nahon, Mathurin Fumery, Benoit Coffin, Philippe Marteau, Matthieu Allez, Guillaume Savoye, Javier P. Gisbert, Martine De Vos, Jean-Yves Mary, Jacques Moreau, Elena Ricart, Laurent Peyrin-Biroulet, Xavier Roblin, Jérôme Filippi, Franck Carbonnel, Maria Esteve, Olivier Dewit, Aurelien Amiot, David Laharie, Martti Färkkilä, Lucine Vuitton, Anthony Buisson, Antonio López-Sanromán, Gert Van Assche, Frank Zerbib, CHU Bordeaux [Bordeaux], CIC Bordeaux, Université Bordeaux Segalen - Bordeaux 2-Institut National de la Santé et de la Recherche Médicale (INSERM), Hôpital Haut-Lévêque [CHU Bordeaux], Institut des Maladies de l'Appareil Digestif, Université de Nantes (UN), Hôtel-Dieu de Nantes, Centre hospitalier universitaire de Nantes (CHU Nantes), Hôpital Claude Huriez [Lille], CHU Lille, Hopital Saint-Louis [AP-HP] (AP-HP), Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), Université Paris Diderot - Paris 7 (UPD7), Hôpital Beaujon [AP-HP], Centre Hospitalier Universitaire de Nice (CHU Nice), Hôpital l'Archet, Nutrition, inflammation et dysfonctionnement de l'axe intestin-cerveau (ADEN), Université de Rouen Normandie (UNIROUEN), Normandie Université (NU)-Normandie Université (NU)-Institut National de la Santé et de la Recherche Médicale (INSERM), UNIROUEN - UFR Santé (UNIROUEN UFR Santé), Normandie Université (NU)-Normandie Université (NU), Service d'Hépato-Gastroentérologie [CHU Rouen], Hôpital Charles Nicolle [Rouen]-Université de Rouen Normandie (UNIROUEN), Normandie Université (NU)-Normandie Université (NU)-CHU Rouen, Normandie Université (NU), Hôpital Charles Nicolle [Rouen], Centre Hospitalier Régional Universitaire de Besançon (CHRU Besançon), Hôpital JeanMinjoz, CHU Toulouse [Toulouse], Hôpital Henri Mondor, Université Paris-Est Créteil Val-de-Marne - Paris 12 (UPEC UP12), Service de Gastroentérologie et nutrition [CHU Saint-Antoine], CHU Saint-Antoine [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), Institut Pierre Louis d'Epidémiologie et de Santé Publique (iPLESP), Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU), Centro de Investigación Biomédica en Red en el Área temática de Enfermedades Hepáticas y Digestivas (CIBERehd), Liver Unit, Clínica Universitaria, CIBER-EHD, Université Catholique de Louvain = Catholic University of Louvain (UCL), Hospital Universitario Ramón y Cajal [Madrid], Universidad de Alcalá - University of Alcalá (UAH), CHU Amiens-Picardie, Périnatalité et Risques Toxiques - UMR INERIS_I 1 (PERITOX), Institut National de l'Environnement Industriel et des Risques (INERIS)-Université de Picardie Jules Verne (UPJV)-CHU Amiens-Picardie, Service d'Hépato-gastro-entérologie [APHP Kremlin-Bicêtre], AP-HP Hôpital Bicêtre (Le Kremlin-Bicêtre), Université Paris-Sud - Paris 11 (UP11), Microbes, Intestin, Inflammation et Susceptibilité de l'Hôte (M2iSH), Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre de Recherche en Nutrition Humaine d'Auvergne (CRNH d'Auvergne)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Université Clermont Auvergne (UCA), Infection Inflammation et Interaction Hôtes Pathogènes [CHU Clermont-Ferrand] (3IHP ), Direction de la recherche clinique et de l’innovation [CHU Clermont-Ferrand] (DRCI), CHU Clermont-Ferrand-CHU Clermont-Ferrand, Hôpital Louis Mourier - AP-HP [Colombes], Centre Hospitalier Universitaire de Saint-Etienne (CHU de Saint-Etienne), Hôpital Nord (Saint Etienne), University Hospitals Leuven [Leuven], University of Helsinki, CHU Tenon [AP-HP], Sorbonne Université (SU), Groupe Hospitalier Intercommunal Le Raincy-Montfermeil, Ghent University Hospital, Nutrition-Génétique et Exposition aux Risques Environnementaux (NGERE), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Lorraine (UL), Centre Hospitalier Régional Universitaire de Nancy (CHRU Nancy), Equipe 2 : ECSTRA - Epidémiologie Clinique, STatistique, pour la Recherche en Santé (CRESS - U1153), Centre de Recherche Épidémiologie et Statistique Sorbonne Paris Cité (CRESS (U1153 / UMR_A_1125 / UMR_S_1153)), Conservatoire National des Arts et Métiers [CNAM] (CNAM), HESAM Université (HESAM)-HESAM Université (HESAM)-Université Sorbonne Paris Cité (USPC)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Paris Cité (UPC)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Conservatoire National des Arts et Métiers [CNAM] (CNAM), HESAM Université (HESAM)-HESAM Université (HESAM)-Université Sorbonne Paris Cité (USPC)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Paris Cité (UPC)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Centre of Excellence in Complex Disease Genetics, HUS Abdominal Center, Department of Medicine, Gastroenterologian yksikkö, Helsinki University Hospital Area, Hôpital Archet 2 [Nice] (CHU), Hôpital Charles Nicolle [Rouen]-CHU Rouen, Normandie Université (NU)-Normandie Université (NU)-Université de Rouen Normandie (UNIROUEN), Service de Gastro-Entérologie [CHRU Besançon], Service de Gastro-entérologie et Nutrition[Rangueil], CHU Toulouse [Toulouse]-Hôpital de Rangueil, Service de gastro-entérologie [Henri Mondor AP-HP, Créteil], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Hôpital Henri Mondor, Sorbonne Université (SU)-Institut National de la Santé et de la Recherche Médicale (INSERM), Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-CHU Saint-Antoine [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU)-Sorbonne Université (SU), Institut d'Investigacions Biomèdiques August Pi i Sunyer (IDIBAPS), Universitat de Barcelona (UB), Hospital Clínic de Barcelona, Cliniques Universitaires Saint-Luc [Bruxelles], Service d'Hépato Gastroenterologie [CHU Amiens-Picardie], Université de Picardie Jules Verne (UPJV)-CHU Amiens-Picardie-Institut National de l'Environnement Industriel et des Risques, Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Clermont Auvergne [2017-2020] (UCA [2017-2020])-Centre de Recherche en Nutrition Humaine d'Auvergne (CRNH d'Auvergne)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Service d'Hépato-gastroentérologie [CHU Saint-Etienne], Hospital Universitario Mutua de Terrassa, Helsinki University Central Hospital [Finland] (HUCH), Universidad Autonoma de Madrid (UAM), Hospital Universitario de la Princesa, Service d'Hépato-gastro-entérologie [CHU Tenon], Sorbonne Université (SU)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), Service d'Hépato-gastro-entérologie [CHRU Nancy], Conservatoire National des Arts et Métiers [CNAM] (CNAM)-Université Sorbonne Paris Cité (USPC)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Paris (UP)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Association Francois Aupetit, CHU Rouen, Pôle Maladies de l'appareil digestif [CHU Toulouse], Centre Hospitalier Universitaire de Toulouse (CHU Toulouse), Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU), Service d'Hépatologie Gastro-entérologie [CHU Clermont-Ferrand], CHU Estaing [Clermont-Ferrand], Centre Hospitalier Universitaire de Saint-Etienne [CHU Saint-Etienne] (CHU ST-E), Helsingin yliopisto = Helsingfors universitet = University of Helsinki, HESAM Université - Communauté d'universités et d'établissements Hautes écoles Sorbonne Arts et métiers université (HESAM)-HESAM Université - Communauté d'universités et d'établissements Hautes écoles Sorbonne Arts et métiers université (HESAM)-Université Sorbonne Paris Cité (USPC)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Paris Cité (UPCité)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Conservatoire National des Arts et Métiers [CNAM] (CNAM), HESAM Université - Communauté d'universités et d'établissements Hautes écoles Sorbonne Arts et métiers université (HESAM)-HESAM Université - Communauté d'universités et d'établissements Hautes écoles Sorbonne Arts et métiers université (HESAM)-Université Sorbonne Paris Cité (USPC)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Paris Cité (UPCité)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), DESSAIVRE, Louise, and Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU)
Subjects: medicine.medical_specialty, MAINTENANCE THERAPY, UCEIS, [SDV]Life Sciences [q-bio], Gastroenterology, Severity of Illness Index, Mucosal Healing, law.invention, Vedolizumab, 03 medical and health sciences, 0302 clinical medicine, Maintenance therapy, Randomized controlled trial, law, Interquartile range, VEDOLIZUMAB, Internal medicine, medicine, Humans, Prospective Studies, Prospective cohort study, INDEX, OUTCOMES, Hepatology, business.industry, ulcerative Colitis, INDUCTION, [SDV.MHEP.HEG]Life Sciences [q-bio]/Human health and pathology/Hépatology and Gastroenterology, REMISSION, medicine.disease, Ulcerative colitis, [SDV.MHEP.HEG] Life Sciences [q-bio]/Human health and pathology/Hépatology and Gastroenterology, Infliximab, 3. Good health, Treatment Outcome, 030220 oncology & carcinogenesis, 3121 General medicine, internal medicine and other clinical medicine, Cyclosporine, 030211 gastroenterology & hepatology, Colitis, Ulcerative, Steroids, business, Steroid refractory, medicine.drug
Abstract: International audience; BACKGROUND/AIMS: Few data on the evolution of endoscopic findings are available in patients with acute severe ulcerative colitis (ASUC). The aim of this study was to describe this evolution in a prospective cohort. METHODS: Patients admitted for a steroid-refractory ASUC and included in a randomized trial comparing infliximab and cyclosporine were eligible if they achieved steroid-free clinical remission at day 98. Flexible sigmoidoscopies were performed at baseline, days 7, 42 and 98. Ulcerative colitis endoscopic index of severity (UCEIS) and its sub-scores - vascular pattern, bleeding and ulceration/erosion - were post-hoc calculated. Global endoscopic remission was defined by a UCEIS of 0, and partial endoscopic remission by any UCEIS sub-score of 0. RESULTS: Among the 55 patients analyzed (29 infliximab and 26 cyclosporine), 49 (83%) had UCEIS >= 6 at baseline at baseline. Partial endoscopic remission rates were higher for bleeding than for vascular pattern and for ulcerations/erosions at day 7 (20% vs. 4% and 5% (n = 55); p
Published: 2021

41. Basal Ti level in the human placenta and meconium and evidence of a materno- foetal transfer of food-grade TiO2 nanoparticles in an ex vivo placental perfusion model

Author: Adèle Guillard, Eric Gaultier, Christel Cartier, Laurent Devoille, Johanna Noireaux, Laurence Chevalier, Mathieu Morin, Grandin, Flore C., Mz Lacroix, Christine Coméra, Aude Cazanave, Alice de Place, Véronique Gayrard-Troy, Véronique Bach, Karen Chardon, Nihel Bekhti, Karine Adel-Patient, Vayssière, C., Fisicaro, P., Feltin, N., La Farge, F., Nicole Picard-Hagen, Bruno Lamas, Eric Houdeau, Endocrinologie & Toxicologie de la Barrière Intestinale (ToxAlim-ENTeRisk), ToxAlim (ToxAlim), Université Toulouse III - Paul Sabatier (UT3), Université de Toulouse (UT)-Université de Toulouse (UT)-Ecole Nationale Vétérinaire de Toulouse (ENVT), Institut National Polytechnique (Toulouse) (Toulouse INP), Université de Toulouse (UT)-Université de Toulouse (UT)-Institut National Polytechnique (Toulouse) (Toulouse INP), Université de Toulouse (UT)-Ecole d'Ingénieurs de Purpan (INP - PURPAN), Université de Toulouse (UT)-Université de Toulouse (UT)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Université Toulouse III - Paul Sabatier (UT3), Université de Toulouse (UT)-Université de Toulouse (UT)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Laboratoire National de Métrologie et d'Essais [Trappes] (LNE ), Laboratoire National de Métrologie et d’Essais (LNE), Groupe de physique des matériaux (GPM), Université de Rouen Normandie (UNIROUEN), Normandie Université (NU)-Normandie Université (NU)-Institut national des sciences appliquées Rouen Normandie (INSA Rouen Normandie), Institut National des Sciences Appliquées (INSA)-Normandie Université (NU)-Institut National des Sciences Appliquées (INSA)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche sur les Matériaux Avancés (IRMA), Université de Caen Normandie (UNICAEN), Normandie Université (NU)-Normandie Université (NU)-École Nationale Supérieure d'Ingénieurs de Caen (ENSICAEN), Normandie Université (NU)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université de Rouen Normandie (UNIROUEN), Normandie Université (NU)-Institut national des sciences appliquées Rouen Normandie (INSA Rouen Normandie), Institut National des Sciences Appliquées (INSA)-Normandie Université (NU)-Institut National des Sciences Appliquées (INSA)-Centre National de la Recherche Scientifique (CNRS)-Université de Caen Normandie (UNICAEN), Normandie Université (NU)-École Nationale Supérieure d'Ingénieurs de Caen (ENSICAEN), Normandie Université (NU)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Centre National de la Recherche Scientifique (CNRS), Centre Hospitalier Universitaire de Toulouse (CHU Toulouse), Innovations Thérapeutiques et Résistances (InTheRes), Ecole Nationale Vétérinaire de Toulouse (ENVT), Neuro-Gastroentérologie & Nutrition (ToxAlim-NGN), Exposition, Perturbation Endocrino-métabolique et Reproduction (ToxAlim-EXPER), Université de Picardie Jules Verne (UPJV), Périnatalité et Risques Toxiques - UMR INERIS_I 1 (PERITOX), Institut National de l'Environnement Industriel et des Risques (INERIS)-Université de Picardie Jules Verne (UPJV)-CHU Amiens-Picardie, Médicaments et Technologies pour la Santé (MTS), Université Paris-Saclay-Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Epidémiologie et analyses en santé publique : risques, maladies chroniques et handicaps (LEASP), Université de Toulouse (UT)-Université de Toulouse (UT)-Institut National de la Santé et de la Recherche Médicale (INSERM), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Ecole Nationale Vétérinaire de Toulouse (ENVT), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Ecole d'Ingénieurs de Purpan (INPT - EI Purpan), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Centre National de la Recherche Scientifique (CNRS)-Institut national des sciences appliquées Rouen Normandie (INSA Rouen Normandie), Institut National des Sciences Appliquées (INSA)-Normandie Université (NU)-Institut National des Sciences Appliquées (INSA)-Normandie Université (NU)-Université de Rouen Normandie (UNIROUEN), Normandie Université (NU), Hôpital Paule de Viguier, CHU Toulouse [Toulouse], Université de Picardie Jules Verne (UPJV)-CHU Amiens-Picardie-Institut National de l'Environnement Industriel et des Risques, and Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National de la Santé et de la Recherche Médicale (INSERM)
Subjects: human placenta, [SDV]Life Sciences [q-bio], Titanium dioxide, nanoparticles, E171 food additive, ComputingMilieux_MISCELLANEOUS
Abstract: International audience
Published: 2020

42. Alteration of predatory behaviour and growth in juvenile cuttlefish by fluoxetine and venlafaxine

Author: Apolline Chabenat, Thomas Knigge, Flavie Bidel, Cécile Bellanger, Stress Environnementaux et BIOsurveillance des milieux aquatiques (SEBIO), Université Le Havre Normandie (ULH), Normandie Université (NU)-Normandie Université (NU)-Université de Reims Champagne-Ardenne (URCA)-SFR Condorcet, Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS)-Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS)-Institut National de l'Environnement Industriel et des Risques (INERIS), Ethologie animale et humaine (EthoS), Université de Caen Normandie (UNICAEN), Normandie Université (NU)-Normandie Université (NU)-Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-Centre National de la Recherche Scientifique (CNRS), Alexander Silberman Institute of Life Science, The Hebrew University of Jerusalem (HUJ), This research work was supported by a doctoral grant from Normandy Region provided by the Research Federation CNRS 3730 SCALE (SCiences Appliquées à L'Environnement), Institut National de l'Environnement Industriel et des Risques (INERIS)-Université de Reims Champagne-Ardenne (URCA)-Université Le Havre Normandie (ULH), Normandie Université (NU)-Normandie Université (NU)-SFR Condorcet, Université de Reims Champagne-Ardenne (URCA)-Centre National de la Recherche Scientifique (CNRS)-Université de Reims Champagne-Ardenne (URCA)-Centre National de la Recherche Scientifique (CNRS), and Normandie Université (NU)-Normandie Université (NU)-Université de Rennes (UR)-Centre National de la Recherche Scientifique (CNRS)
Subjects: Cuttlefish, Environmental Engineering, food intake, Health, Toxicology and Mutagenesis, 0208 environmental biotechnology, Prey detection, Population, Zoology, 02 engineering and technology, 010501 environmental sciences, Biology, Sepia officinalis, 01 natural sciences, Predation, monoamines, Fluoxetine, Environmental Chemistry, Juvenile, Animals, 14. Life underwater, Sepia, education, Hatchling, cephalopod, development, 0105 earth and related environmental sciences, education.field_of_study, antidepressant, Hatching, maturation, [SCCO.NEUR]Cognitive science/Neuroscience, Public Health, Environmental and Occupational Health, Decapodiformes, Venlafaxine Hydrochloride, General Medicine, General Chemistry, Pollution, 020801 environmental engineering, [SDV.BA.ZI]Life Sciences [q-bio]/Animal biology/Invertebrate Zoology, Predatory Behavior, [SDV.TOX.ECO]Life Sciences [q-bio]/Toxicology/Ecotoxicology, Water Pollutants, Chemical
Abstract: International audience; Antidepressants in coastal waters may affect ontogeny of predatory behaviour in cuttlefish, which may, as a result, affect growth of newly-hatched cuttlefish. We investigated the effects of two of the most prescribed antidepressants, fluoxetine (FLX) and venlafaxine (VEN) in environmentally realistic concentrations on the predatory behaviour of hatchlings of Sepia officinalis. Newly-hatched cuttlefish were exposed from 1 hour (i.e., day 1) to 5 days after hatching to either FLX alone (5 ng·L-1) combined with VEN (2.5 ng·L-1 or 5 ng·L-1 each) to simulate an environmentally realistic exposure scenario. Their predatory behaviour was analysed through several parameters: prey detection, feeding motivation and success in catching the prey. All parameters improved in control animals over the first five days. The combination of FLX and VEN at 5 ng·L-1 each altered the predatory behaviour of the hatchlings by increasing the latency before attacking the prey, i.e., reducing feeding motivation, as well as by reducing the number of successful attacks. The changes in predatory behaviour tended to reduce food intake and affected growth significantly at 28 days post-hatching. Exposures to either FLX at 5 ng·L-1 or FLX and VEN in mixture at 2.5 ng·L-1 each tended to produce similar effects, even though they were not statistically significant. It is likely that the antidepressants affect maturation of the predatory behaviour and/or learning processes associated with the development of this behaviour. The slightest delay in maturation processes may have detrimental consequences for growth and population fitness.
Published: 2020

43. The Use of sp-ICP-MS to Study the Behavior of Inhaled TiO2 NPs in Lung's Compartments

Author: Ben Dhiab, Ibtihel, Peyret, Emmanuel, Lecomte, Anthony, Robidel, Franck, Papin, Arnaud, Aguerre-Chariol, Olivier, Trouiller, Bénédicte, Lacroix, Ghislaine, Institut National de l'Environnement Industriel et des Risques (INERIS), Périnatalité et Risques Toxiques - UMR INERIS_I 1 (PERITOX), and Université de Picardie Jules Verne (UPJV)-CHU Amiens-Picardie-Institut National de l'Environnement Industriel et des Risques
Subjects: integumentary system, [SDV.TOX]Life Sciences [q-bio]/Toxicology, [SDE]Environmental Sciences, respiratory system
Abstract: The purpose of the work was to assess the contribution of sp-ICP-MS for nanoparticle (NP) biodistribution studies, with the exemple of inhaled TiO2 NPs fate in rat lungs.
Published: 2020

44. Peut-on comparer l’excès de risque de cancer induit par une exposition aux substances chimiques au détriment radiologique estimé lors d’une exposition aux rayonnements ionisants ?

Author: CLERO, Enora, Bisson, Michèle, BILLARAND, Yann, Velly, Nathalie, Thybaud, Eric, PSE-SANTE/SESANE/LEPID, Institut de Radioprotection et de Sûreté Nucléaire (IRSN), Institut national de l’environnement industriel et des risques (INERIS), PSE-ENV/SEDDER, and Institut National de l'Environnement Industriel et des Risques (INERIS)
Subjects: [SDV]Life Sciences [q-bio]
Abstract: International audience; Contexte Pour se conformer au cadre réglementaire, des évaluations d’impact sanitaire sont réalisées pour les installations susceptibles de rejeter des substances radioactives et chimiques, en vue d’obtenir l’autorisation de les exploiter. Lors d’exposition à des niveaux faibles, de plus en plus d’experts sont tentés d’utiliser une approche graduelle ou proportionnée pour comparer le risque radiologique au risque chimique.Méthode Les méthodes de calcul de risques sanitaires chimiques et radiologiques seront présentées pour des effets sans seuil, essentiellement des effets cancérogènes. Les différentes étapes constitutives de chacune des deux approches seront détaillées de manière séquentielle : origine des données d’incidence des cancers, méthodes de raisonnement utilisées et processus de calcul.Résultats Concernant le risque chimique, la méthode s’appuie sur la détermination de valeurs toxicologiques de référence pour des effets sans seuil, les excès de risques unitaires, pour chacune des voies d’exposition de chaque substance concernée puis d’une sommation des risques pour l’ensemble des substances du mélange. Concernant le risque radiologique, le concept de détriment radiologique utilisé par la Commission Internationale de Protection Radiologique permet de quantifier l’ensemble des effets stochastiques nocifs de l’exposition aux rayonnements ionisants à faible dose et faible débit de dose. Il est déterminé à partir des coefficients de risques nominaux par type de cancer (organe/tissu), en tenant compte de la gravité de la maladie en termes de létalité, de qualité de vie et d’années de vie perdues.Conclusion En conclusion, il s’agit d’une analyse détaillée des deux approches mettant en exergue les différents éléments pour une meilleure compréhension des démarches et de leur interprétation. Les deux approches de calcul du risque chimique et radiologique seront mises en parallèle afin de déterminer à quel niveau elles sont comparables.
Published: 2020

45. Application des méthodes d’évaluation des risques cumulés pour l'identification des zones de surexposition

Author: Caudeville, Julien, Institut National de l'Environnement Industriel et des Risques (INERIS), Périnatalité et Risques Toxiques - UMR INERIS_I 1 (PERITOX), and Université de Picardie Jules Verne (UPJV)-CHU Amiens-Picardie-Institut National de l'Environnement Industriel et des Risques
Subjects: [SDV.EE.SANT]Life Sciences [q-bio]/Ecology, environment/Health
Published: 2020

46. Characterizing environmental geographic inequalities using an integrated exposure assessment

Author: Laure Malherbe, Mohammed Guedda, Céline Brochot, Roseline Bonnard, Karen Chardon, Julien Caudeville, Maxime Beauchamp, Corentin Regrain, Laurent Létinois, François Lestremau, Frédéric Tognet, Fabrice Marliere, Florence Anna Zeman, Véronique Bach, Institut National de l'Environnement Industriel et des Risques (INERIS), Périnatalité et Risques Toxiques - UMR INERIS_I 1 (PERITOX), Université de Picardie Jules Verne (UPJV)-CHU Amiens-Picardie-Institut National de l'Environnement Industriel et des Risques, Laboratoire Amiénois de Mathématique Fondamentale et Appliquée - UMR CNRS 7352 (LAMFA), and Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS)
Subjects: medicine.medical_specialty, Inequities, Insecticides, Geographic information system, Computer science, Health, Toxicology and Mutagenesis, Population, Food Contamination, 010501 environmental sciences, 01 natural sciences, Representativeness heuristic, Benzoates, Risk Assessment, Exposure, Integrated, 11. Sustainability, Nitriles, Pyrethrins, medicine, Spatial, Humans, education, 0105 earth and related environmental sciences, Exposure assessment, [SDV.EE.SANT]Life Sciences [q-bio]/Ecology, environment/Health, education.field_of_study, Operationalization, business.industry, Public health, Public Health, Environmental and Occupational Health, Methodology, Modeling, 04 agricultural and veterinary sciences, Environmental Exposure, Models, Theoretical, Industrial medicine. Industrial hygiene, RC963-969, Risk analysis (engineering), 13. Climate action, Scale (social sciences), 040103 agronomy & agriculture, Geographic Information Systems, 0401 agriculture, forestry, and fisheries, [SDV.SPEE]Life Sciences [q-bio]/Santé publique et épidémiologie, Public aspects of medicine, RA1-1270, business, Environmental Health
Abstract: Background At a regional or continental scale, the characterization of environmental health inequities (EHI) expresses the idea that populations are not equal in the face of pollution. It implies an analysis be conducted in order to identify and manage the areas at risk of overexposure where an increasing risk to human health is suspected. The development of methods is a prerequisite for implementing public health activities aimed at protecting populations. Methods This paper presents the methodological framework developed by INERIS (French National Institute for Industrial Environment and Risks) to identify a common framework for a structured and operationalized assessment of human exposure. An integrated exposure assessment approach has been developed to integrate the multiplicity of exposure pathways from various sources, through a series of models enabling the final exposure of a population to be defined. Results Measured data from environmental networks reflecting the actual contamination of the environment are used to gauge the population’s exposure. Sophisticated methods of spatial analysis are applied to include additional information and take benefit of spatial and inter-variable correlation to improve data representativeness and characterize the associated uncertainty. Integrated approaches bring together all the information available for assessing the source-to-human-dose continuum using a Geographic Information System, multimedia exposure and toxicokinetic model. Discussion One of the objectives of the integrated approach was to demonstrate the feasibility of building complex realistic exposure scenarios satisfying the needs of stakeholders and the accuracy of the modelling predictions at a fine spatial-temporal resolution. A case study is presented to provide a specific application of the proposed framework and how the results could be used to identify an overexposed population. Conclusion This framework could be used for many purposes, such as mapping EHI, identifying vulnerable populations and providing determinants of exposure to manage and plan remedial actions and to assess the spatial relationships between health and the environment to identify factors that influence the variability of disease patterns.
Published: 2020

47. Influence of chronic radiofrequency electromagnetic fields exposure on autonomic nervous activity during sleep in preterm neonates : preliminary results

Author: Besset, D., Selmaoui, B., Delanaud, S., René de Seze, Leke, A., Stephan-Blanchard, E., Périnatalité et Risques Toxiques - UMR INERIS_I 1 (PERITOX), Université de Picardie Jules Verne (UPJV)-CHU Amiens-Picardie-Institut National de l'Environnement Industriel et des Risques, and Institut National de l'Environnement Industriel et des Risques (INERIS)
Subjects: [SDV.TOX]Life Sciences [q-bio]/Toxicology
Abstract: Autonomic nervous control during sleep play a key role in the maintenance of homeostasis in preterm neonates. Low-frequency electromagnetic (0-100 kHz) fields have been associated with alteration in such mechanisms. However, it has never been tested with high frequencies whereas preterm neonates are also chronically exposed to radiofrequencies (RF, 87.5 MHz – 5.8 GHz) while hospitalized. We investigated the influence of chronic RF exposure on autonomic nervous activity in sleeping preterm neonates...
Published: 2020

48. Design of an Integrated Platform for Mapping Residential Exposure to Rf-Emf Sources

Author: Regrain, Corentin, Caudeville, Julien, de Seze, René, Guedda, Mohammed, Chobineh, Amirreza, de Doncker, Philippe, Petrillo, Luca, Chiaramello, Emma, Parazzini, Marta, Joseph, Wout, Aerts, Sam, Huss, Anke, Wiart, Joe, IRAS OH Epidemiology Chemical Agents, dIRAS RA-2, Institut National de l'Environnement Industriel et des Risques (INERIS), Périnatalité et Risques Toxiques - UMR INERIS_I 1 (PERITOX), Université de Picardie Jules Verne (UPJV)-CHU Amiens-Picardie-Institut National de l'Environnement Industriel et des Risques, Laboratoire Traitement et Communication de l'Information (LTCI), Institut Mines-Télécom [Paris] (IMT)-Télécom Paris, Télécom Paris, Chaire Modélisation, Caractérisation et Maîtrise des expositions aux ondes électromagnétiques (C2M), IMT Atlantique Bretagne-Pays de la Loire (IMT Atlantique), Institut Mines-Télécom [Paris] (IMT)-Institut Mines-Télécom [Paris] (IMT)-Télécom ParisTech, Faculté des Sciences [Bruxelles] (ULB), Université libre de Bruxelles (ULB), Département Communications & Electronique (COMELEC), Télécom ParisTech, IRAS OH Epidemiology Chemical Agents, and dIRAS RA-2
Subjects: SURROGATE, spatiotemporal exposure, Monte Carlo approach, Radio Waves, Health, Toxicology and Mutagenesis, assessment, lcsh:Medicine, spatiotemporal exposure assessment, YOUNG-PEOPLE, CHILDREN, 02 engineering and technology, 010501 environmental sciences, 01 natural sciences, [SPI]Engineering Sciences [physics], 0202 electrical engineering, electronic engineering, information engineering, POPULATION, ComputingMilieux_MISCELLANEOUS, education.field_of_study, Communication, BUILDINGS, Specific absorption rate, Grid, 3. Good health, ELECTROMAGNETIC-FIELD EXPOSURE, Whole body, animal structures, Technology and Engineering, Real-time computing, Population, BASE STATION EXPOSURE, Sciences de l'ingénieur, Article, Electromagnetic Fields, Humans, EXPOSURE, education, 0105 earth and related environmental sciences, Exposure assessment, data fusion, business.industry, lcsh:R, Public Health, Environmental and Occupational Health, 020206 networking & telecommunications, Environmental Exposure, Sensor fusion, MODEL, radiofrequency electromagnetic fields, Environmental science, Radiation protection, business, Cell Phone, ENVIRONMENTS
Abstract: Nowadays, information and communication technologies (mobile phones, connectedobjects) strongly occupy our daily life. The increasing use of these technologies and the complexity ofnetwork infrastructures raise issues about radiofrequency electromagnetic fields (Rf-Emf) exposure.Most previous studies have assessed individual exposure to Rf-Emf, and the next level is to assesspopulational exposure. In our study, we designed a statistical tool for Rf-Emf populational exposureassessment and mapping. This tool integrates geographic databases and surrogate models tocharacterize spatiotemporal exposure from outdoor sources, indoor sources, and mobile phones.A case study was conducted on a 100 x 100 m grid covering the 14th district of Paris to illustratethe functionalities of the tool. Whole-body specific absorption rate (SAR) values are 2.7 times higherthan those for the whole brain. The mapping of whole-body and whole-brain SAR values showsa dichotomy between built-up and non-built-up areas, with the former displaying higher values.Maximum SAR values do not exceed 3.5 and 3.9 mW/kg for the whole body and the whole brain,respectively, thus they are significantly below International Commission on Non-Ionizing RadiationProtection (ICNIRP) recommendations. Indoor sources are the main contributor to populationalexposure, followed by outdoor sources and mobile phones, which generally represents less than 1%of total exposure., info:eu-repo/semantics/published
Published: 2020

49. Assessment of water quality through an active biomonitoring approach using three-spined stickleback (Gasterosteus aculeatus)

Author: Catteau, Audrey, Bado-Nilles, Anne, Beaudouin, Rémy, Tebby, Cléo, Joachim, Sandrine, Palluel, Olivier, Turies, Cyril, Chretien, Nina, Geffard, Alain, Porcher, Jean-Marc, Institut National de l'Environnement Industriel et des Risques (INERIS), Stress Environnementaux et BIOsurveillance des milieux aquatiques (SEBIO), Université Le Havre Normandie (ULH), Normandie Université (NU)-Normandie Université (NU)-Université de Reims Champagne-Ardenne (URCA)-SFR Condorcet, and Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS)-Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS)-Institut National de l'Environnement Industriel et des Risques (INERIS)
Subjects: [SDV.TOX]Life Sciences [q-bio]/Toxicology, [SDV.TOX.ECO]Life Sciences [q-bio]/Toxicology/Ecotoxicology
Abstract: The Interreg DIADeM program (development of an integrated approach for the DIAgnosis of the water quality of the river Meuse) was created in order to develop new tools to evaluate the quality of water bodies in the Meuse waterway across the FrenchBelgian border. The project aimed to bring together the various crossborder stakeholders concerned with the protection of aquatic environments (academics, operators, users) while informing citizens of the risks caused by the degradation of water bodies. The use and the relevance of the multibiomarker approach in different model species (fish, crustacean, mussel) to assess the water quality was an important part of the program. Among the different model species used, the threespined stickleback has gained interest for biomonitoring for several years, due to its ubiquity and its tolerance to pollution. For many years, a new active strategy based on the importation of caged sticklebacks in a study site have been developed. Within the scope of the DIADeM program, the caging strategy was used with stickleback as model species to discriminate some sites in a large scale on the Meuse basin. In that way, sticklebacks were caged for 21 days upstream and downstream the wastewater treatment plants (WWTPs) of Namur (Belgiun), CharlevilleMézière (France), Bouillon (Belgium) and Avesnes-sur-Helpe (France). A battery of biomarkers was assessed to represent different physiological functions, such as innate immunity (leucocyte mortality and distribution, phagocytosis activity, respiratory burst, antioxidant system (GPx, CAT, SOD and GSH content) and oxidative damages (TBARS), biotransformation enzymes (EROD, GST), synaptic transmission (AChE) and reproduction system (spiggin and vitellogenin concentration). Physiological responses were compared between each site but also with biomarkers levels measured in sticklebacks under control conditions. Study highlighted a decrease of innate immune system downstream the WWTPs, especially through an increase of leucocyte mortality and a decrease in phagocytosis activity. A slight induction of EROD activity was also noticed downstream the WWTP of Bouillon and Namur. Moreover, the use of active biomonitoring clearly discriminated the different study sites from the reference but also amongst themselves, demonstrating the efficiency of used tools to assess the quality of water and characterize types of physiological impacts on fish.
Published: 2020

50. Usefulness of the transgenic cyp19a1b-GFP zebrafish model to refine the zebrafish embryo acute toxicity test (FET)

Author: Armelle Christophe, Marc Leonard, Pascal PANDARD, Noémie de Croze, François Brion, Institut National de l'Environnement Industriel et des Risques (INERIS), Stress Environnementaux et BIOsurveillance des milieux aquatiques (SEBIO), Université Le Havre Normandie (ULH), Normandie Université (NU)-Normandie Université (NU)-Université de Reims Champagne-Ardenne (URCA)-SFR Condorcet, and Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS)-Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS)-Institut National de l'Environnement Industriel et des Risques (INERIS)
Subjects: [SDV.TOX.ECO]Life Sciences [q-bio]/Toxicology/Ecotoxicology
Published: 2020

Catalog

Books, media, physical & digital resources

See catalog results

Searchworks

Select search scope, currently: Articles Catalog books, media & more in Jio Institute collections Articles journal articles & other e-resources

Search

Search Constraints

Refine your results

Search Limiters

Topic

Publication Year Range

Language

Publication Type

Journal

Region

Database

Publisher

5,142 results on '"Institut national de l'environnement industriel et des risques"'

Search Results

Catalog

Select search scope, currently: Articles

Catalog

books, media & more in Jio Institute collections

Articles

journal articles & other e-resources