1. Gas-phase protein inner-shell spectroscopy by coupling an ion trap with a soft x-ray beamline
- Author
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Christophe Nicolas, Alexandre Giuliani, Laurent Nahon, Catalin Miron, Francis Canon, Aleksandar R. Milosavljević, Inst Phys, University of Belgrade [Belgrade], Synchrotron SOLEIL (SSOLEIL), Centre National de la Recherche Scientifique (CNRS), Centre des Sciences du Goût et de l'Alimentation [Dijon] (CSGA), Institut National de la Recherche Agronomique (INRA)-Université de Bourgogne (UB)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Centre National de la Recherche Scientifique (CNRS), Département Caractérisation et Elaboration des Produits Issus de l'Agriculture (CEPIA), Institut National de la Recherche Agronomique (INRA), Agence Nationale de la Recherche Scientifique [BLAN08-1_348053], Institut Français de Serbie through the EGIDE, Ministry of Education and Science of the Republic of Serbia [171020], Synchrotron SOLEIL ( SSOLEIL ), Centre National de la Recherche Scientifique ( CNRS ), Centre des Sciences du Goût et de l'Alimentation [Dijon] ( CSGA ), Institut National de la Recherche Agronomique ( INRA ) -Université de Bourgogne ( UB ) -AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Centre National de la Recherche Scientifique ( CNRS ), Département Caractérisation et Elaboration des Produits Issus de l'Agriculture ( CEPIA ), and Institut National de la Recherche Agronomique ( INRA )
- Subjects
NEXAFS SPECTROSCOPY ,Double ionization ,Analytical chemistry ,Photoelectron photoion coincidence spectroscopy ,02 engineering and technology ,Photoionization ,010402 general chemistry ,Mass spectrometry ,01 natural sciences ,Ion ,REGION ,Ionization ,PHOTOIONIZATION ,EXCITATION ,SPECTRA ,General Materials Science ,Physical and Theoretical Chemistry ,Quadrupole ion trap ,PHOTOEMISSION ,FUNCTIONAL PROTEINS ,Chemistry ,GLYCINE ,MASS-SPECTROMETRY ,021001 nanoscience & nanotechnology ,0104 chemical sciences ,[CHIM.THEO]Chemical Sciences/Theoretical and/or physical chemistry ,[ CHIM.THEO ] Chemical Sciences/Theoretical and/or physical chemistry ,Ion trap ,0210 nano-technology ,DECAY - Abstract
C, N, and O near-edge ion yield spectroscopy of 8+ selected electrosprayed cations of cytochrome c protein (12 kDa) has been performed by coupling a linear quadrupole ion trap with a soft X-ray beamline. The photoactivation tandem mass spectra were recorded as a function of the photon energy. Photoionization of the precursor, accompanied by CO2 loss, is the dominant relaxation process, showing high photoion stability following direct or resonant photoionization. The partial ion yields extracted from recorded mass spectra show significantly different behaviors for single and double ionization channels, which can be qualitatively explained by different Auger decay mechanisms. However, the single ionization spectra reveal characteristic structures when compared to existing near-edge X-ray absorption fine structure (NEXAFS) spectra from thin films of peptides and proteins. Therefore, the present experiment opens up new avenues for near-edge X-ray spectroscopy of macromolecules in the gas phase, overcoming the radiation damage issue or the environmental effects as due to the surface, intermolecular interactions, and solvent.
- Published
- 2012