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1. An HMM approach expands the landscape of sesquiterpene cyclases across the kingdom Fungi

Author

Hayat Hage, Julie Couillaud, Asaf Salamov, Margot Loussouarn-Yvon, Fabien Durbesson, Elena Ormeño, Sacha Grisel, Katia Duquesne, Renaud Vincentelli, Igor Grigoriev, Gilles Iacazio, Marie-Noëlle Rosso, Biodiversité et Biotechnologie Fongiques (BBF), Aix Marseille Université (AMU)-École Centrale de Marseille (ECM)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Institut des Sciences Moléculaires de Marseille (ISM2), Aix Marseille Université (AMU)-École Centrale de Marseille (ECM)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Lawrence Berkeley National Laboratory [Berkeley] (LBNL), Architecture et fonction des macromolécules biologiques (AFMB), Aix Marseille Université (AMU)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Institut méditerranéen de biodiversité et d'écologie marine et continentale (IMBE), Avignon Université (AU)-Aix Marseille Université (AMU)-Institut de recherche pour le développement [IRD] : UMR237-Centre National de la Recherche Scientifique (CNRS), Institut des Sciences Moléculaires (ISM), Université Montesquieu - Bordeaux 4-Université Sciences et Technologies - Bordeaux 1 (UB)-École Nationale Supérieure de Chimie et de Physique de Bordeaux (ENSCPB)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Institut Carnot 3BCAR, INRAE, MICA Division, Ecole Doctorale Sciences Chimiques ED 250 - Aix Marseille Université, A*midex Foundation, IM2B - Institute of Microbiology, Bioenergies and Biotechnology, Department of Energy Joint Genome Institute, and IMBE - Institut Méditerranéen de Biodiversité et d'Ecologie marine et continentale

Subjects
sesquiterpene, [SDV.BBM.GTP]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Genomics [q-bio.GN], terpene synthase, General Medicine, fungi, [SDV.BBM.BC]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Biochemistry [q-bio.BM], Polyporales, genome, [SDV.MP.MYC]Life Sciences [q-bio]/Microbiology and Parasitology/Mycology
Abstract

Sesquiterpene cyclases (STC) catalyse the cyclization of the C15 molecule farnesyl diphosphate into a vast variety of mono- or polycyclic hydrocarbons and, for a few enzymes, oxygenated structures, with diverse stereogenic centres. The huge diversity in sesquiterpene skeleton structures in nature is primarily the result of the type of cyclization driven by the STC. Despite the phenomenal impact of fungal sesquiterpenes on the ecology of fungi and their potentials for applications, the fungal sesquiterpenome is largely untapped. The identification of fungal STC is generally based on protein sequence similarity with characterized enzymes. This approach has improved our knowledge on STC in a few fungal species, but it has limited success for the discovery of distant sequences. Besides, the tools based on secondary metabolite biosynthesis gene clusters have shown poor performance for terpene cyclases. Here, we used four sets of sequences of fungal STC that catalyse four types of cyclization, and specific amino acid motives to identify phylogenetically related sequences in the genomes of basidiomycetes fungi from the order Polyporales. We validated that four STC genes newly identified from the genome sequence of Leiotrametes menziesii, each classified in a different phylogenetic clade, catalysed a predicted cyclization of farnesyl diphosphate. We built HMM models and searched STC genes in 656 fungal genomes genomes. We identified 5605 STC genes, which were classified in one of the four clades and had a predicted cyclization mechanism. We noticed that the HMM models were more accurate for the prediction of the type of cyclization catalysed by basidiomycete STC than for ascomycete STC.

Published
2023

2. Acyl-CoA:lysophosphatidylcholine acyltransferase from the unicellular diatom Phaeodactylum tricornutum (PtLPCAT1) is involved in triacylglycerol and galactoglycerolipid synthesis and enhances eicosapentaenoic acid accumulation in recombinant oleaginous yeast

Author

You, Lingjie, Jouhet, Juliette, Maréchal, Eric, Amato, Alberto, Hao, Xiahui, Zhang, Donghui, Banaś, Antoni, Gong, Yangmin, Chinese Academy of Agricultural Sciences (CAAS), LIPID, Physiologie cellulaire et végétale (LPCV), Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche Interdisciplinaire de Grenoble (IRIG), Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Université Grenoble Alpes (UGA)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche Interdisciplinaire de Grenoble (IRIG), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Université Grenoble Alpes (UGA), Yangtze University, Intercollegiate Faculty of Biotechnology, Medical University of Gdańsk, grant from the National Natural Science Foundation of China n° 31961133008, NCN, Poland, No. UMO-2018/30/Q/NZ3/00497, IDEX UGA CDP Glyco@Alps, Institut Carnot 3BCAR, ANR-10-LABX-0049,GRAL,Grenoble Alliance for Integrated Structural Cell Biology(2010), and ANR-11-BTBR-0008,OCEANOMICS,Biotechnologies et bioressources pour la valorisation des écosystèmes marins planctoniques(2011)

Subjects
Yarrowia lipolytica, MESH: Triglycerides, eicosapentaenoic acid, [SDV.BIO]Life Sciences [q-bio]/Biotechnology, MESH: Eicosapentaenoic Acid, CRISPR/Cas9 eicosapentaenoic acid LPCAT Phaeodactylum tricornutum Yarrowia lipolytica, LPCAT, MESH: Acyltransferases, Phaeodactylum tricornutum, [SDV.BV.AP]Life Sciences [q-bio]/Vegetal Biology/Plant breeding, MESH: 1-Acylglycerophosphocholine O-Acyltransferase, MESH: Acyl Coenzyme A, CRISPR/Cas9, MESH: Diatoms
Abstract

International audience

Published
2022

3. Editorial: Ice and Snow Algae

Author

Eric Maréchal, Linda Nedbalová, LIPID, Physiologie cellulaire et végétale (LPCV), Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche Interdisciplinaire de Grenoble (IRIG), Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Université Grenoble Alpes (UGA)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche Interdisciplinaire de Grenoble (IRIG), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Université Grenoble Alpes (UGA), Charles University [Prague] (CU), Institut Carnot 3BCAR, Kilian Jornet Foundation, ANR-18-CE92-0015,BLinK,Les bétaines lipides chez les eucaryotes(2018), ANR-20-CE02-0020,ALPALGA,Microalgues des Alpes(2020), ANR-15-IDEX-0002,UGA,IDEX UGA(2015), ANR-10-LABX-0049,GRAL,Grenoble Alliance for Integrated Structural Cell Biology(2010), and ANR-17-EURE-0003,CBH-EUR-GS,CBH-EUR-GS(2017)

Subjects
Sanguina, red snow, glacier, bioalbedo, mountain, Plant Science, [SDV.BV.BOT]Life Sciences [q-bio]/Vegetal Biology/Botanics, [SDE.BE]Environmental Sciences/Biodiversity and Ecology, ice alga, ComputingMilieux_MISCELLANEOUS, snow alga
Abstract

International audience

Published
2022

4. Origin of cyanobacterial thylakoids via a non-vesicular glycolipid phase transition and their impact on the Great Oxygenation Event

Author

Nolwenn Guéguen, Eric Maréchal, LIPID, Physiologie cellulaire et végétale (LPCV), Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche Interdisciplinaire de Grenoble (IRIG), Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Université Grenoble Alpes (UGA)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche Interdisciplinaire de Grenoble (IRIG), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Université Grenoble Alpes (UGA), Institut Carnot 3BCAR, ANR-20-CE02-0020,ALPALGA,Microalgues des Alpes(2020), ANR-11-BTBR-0008,OCEANOMICS,Biotechnologies et bioressources pour la valorisation des écosystèmes marins planctoniques(2011), ANR-15-IDEX-0002,UGA,IDEX UGA(2015), ANR-10-LABX-0049,GRAL,Grenoble Alliance for Integrated Structural Cell Biology(2010), and ANR-17-EURE-0003,CBH-EUR-GS,CBH-EUR-GS(2017)

Subjects
Cyanobacteria, Gloeobacter, Physiology, thylakoid, Plant Science, monogalactosyldiacylglycerol, sulfoquinovosyldiacylglycerol, Photosynthesis, Thylakoids, [SDV.BBM]Life Sciences [q-bio]/Biochemistry, Molecular Biology, phosphatidylglycerol, Photosystem, biology, Chemistry, Great Oxygenation Event, Galactolipids, MGD, biology.organism_classification, [SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology, monoglucosyldiacylglycerol, Thylakoid, Biophysics, lipids (amino acids, peptides, and proteins), Hexagonal II lipid, Biogenesis
Abstract

The appearance of oxygenic photosynthesis in cyanobacteria is a major event in evolution. It had an irreversible impact on the Earth, promoting the Great Oxygenation Event (GOE) ~2.4 billion years ago. Ancient cyanobacteria predating the GOE were Gloeobacter-type cells lacking thylakoids, which hosted photosystems in their cytoplasmic membrane. The driver of the GOE was proposed to be the transition from unicellular to filamentous cyanobacteria. However, the appearance of thylakoids expanded the photosynthetic surface to such an extent that it introduced a multiplier effect, which would be more coherent with an impact on the atmosphere. Primitive thylakoids self-organize as concentric parietal uninterrupted multilayers. There is no robust evidence for an origin of thylakoids via a vesicular-based scenario. This review reports studies supporting that hexagonal II-forming glucolipids and galactolipids at the periphery of the cytosolic membrane could be turned, within nanoseconds and without any external source of energy, into membrane multilayers. Comparison of lipid biosynthetic pathways shows that ancient cyanobacteria contained only one anionic lamellar-forming lipid, phosphatidylglycerol. The acquisition of sulfoquinovosyldiacylglycerol biosynthesis correlates with thylakoid emergence, possibly enabling sufficient provision of anionic lipids to trigger a hexagonal II-to-lamellar phase transition. With this non-vesicular lipid-phase transition, a framework is also available to re-examine the role of companion proteins in thylakoid biogenesis.

Published
2022

5. Multiplex CRISPR/Cas9 editing of the long‐chain acyl‐CoA synthetase family in the diatom Phaeodactylum tricornutum reveals that mitochondrial ptACSL3 is involved in the synthesis of storage lipids

Author

Xiahui Hao, Wenchao Chen, Alberto Amato, Juliette Jouhet, Eric Maréchal, Daniel Moog, Hanhua Hu, Hu Jin, Lingjie You, Fenghong Huang, Mark Moosburner, Andrew E. Allen, Yangmin Gong, Oil Crops Research Institute of the Chinese Academy of Agricultural Sciences Ministry of Agriculture, Key Laboratory of Biology and Genetic Improvement of Oil Crops, LIPID, Physiologie cellulaire et végétale (LPCV), Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche Interdisciplinaire de Grenoble (IRIG), Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Université Grenoble Alpes (UGA)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche Interdisciplinaire de Grenoble (IRIG), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Université Grenoble Alpes (UGA), Laboratory for Cell Biology, Philipps-University Marburg, Laboratory of Algal Biology, Institute of Hydrobiology, Chinese Academy of Sciences, Center for Microalgal Biotechnology and Biofuels, Institute of Hydrobiology, Chinese Academy of Sciences, Scripps Institution of Oceanography (SIO), University of California [San Diego] (UC San Diego), University of California-University of California, J. Craig Venter Institute [La Jolla, USA] (JCVI), National Natural Science Foundation of China (41776175 and 31961133008), Agricultural Science and Technology Innovation Project of Chinese Academy of Agricultural Sciences (CAAS-ASTIP-2013-OCRI), 2021 CEA-CAS bilateral program, Institut Carnot 3BCAR, Grants from the United States Department of Energy Genomics Science program, US National Science Foundation, NSF-MCB-1818390, Gordon and Betty Moore Foundation GBMF3828, ANR-10-LABX-0049,GRAL,Grenoble Alliance for Integrated Structural Cell Biology(2010), ANR-11-BTBR-0008,OCEANOMICS,Biotechnologies et bioressources pour la valorisation des écosystèmes marins planctoniques(2011), ANR-15-IDEX-0002,UGA,IDEX UGA(2015), Scripps Institution of Oceanography (SIO - UC San Diego), and University of California (UC)-University of California (UC)

Subjects
0106 biological sciences, Diatoms, 0303 health sciences, Physiology, Fatty Acids, Multiplexed CRISPR/Cas9, Plant Science, Fatty acid, Long-chain acyl-CoA synthetase, 01 natural sciences, Phaeodactylum tricornutum, Triacylglycerol, Mitochondria, 03 medical and health sciences, Coenzyme A Ligases, Coenzyme A, [SDV.BBM]Life Sciences [q-bio]/Biochemistry, Molecular Biology, CRISPR-Cas Systems, 030304 developmental biology, 010606 plant biology & botany
Abstract

International audience; Long-chain acyl-CoA synthetases (LACSs) play diverse and fundamentally important roles in lipid metabolism. While their functions have been well-established in bacteria, yeast and plants, the mechanisms by which LACS isozymes regulate lipid metabolism in unicellular oil-producing microalgae, including the diatom Phaeodactylum tricornutum, remain largely unknown. In P. tricornutum, a family of five genes (ptACSL1~5) encodes LACS activities. We generated single lacs knockout/knockdown mutants using multiplexed CRISPR/Cas9 method, and determined their substrate specificities towards different fatty acids (FAs) and subcellular localizations. ptACSL3 is localized in the mitochondria and its disruption led to compromised growth and reduced triacylglycerol (TAG) content when cells were bubbled with air. The ptACSL3 mutants showed altered FA profiles in two galactoglycerolipids and phosphatidylcholine (PC) with significantly reduced distribution of 16:0 and 16:1. ptACSL5 is localized in the peroxisome and its knockdown resulted in reduced growth rate and altered molecular species of PC and TAG, indicating a role in controlling the composition of acyl-CoAs for lipid synthesis. Our work demonstrates the potential of generating gene knockout mutants with the mutation of large fragment deletion using multiplexed CRISPR/Cas9 and provides insight into the functions of LACS isozymes in lipid metabolism in the oleaginous microalgae.

Published
2021

6. Grand Challenges in Microalgae Domestication

Author

Eric Maréchal, LIPID, Physiologie cellulaire et végétale (LPCV), Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche Interdisciplinaire de Grenoble (IRIG), Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Université Grenoble Alpes (UGA)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche Interdisciplinaire de Grenoble (IRIG), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Université Grenoble Alpes (UGA), Institut de Recherche Interdisciplinaire de Grenoble (IRIG), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA), Institut Carnot 3BCAR, ANR-20-CE02-0020,ALPALGA,Microalgues des Alpes(2020), ANR-11-BTBR-0008,OCEANOMICS,Biotechnologies et bioressources pour la valorisation des écosystèmes marins planctoniques(2011), ANR-15-IDEX-0002,UGA,IDEX UGA(2015), and ANR-17-EURE-0003,CBH-EUR-GS,CBH-EUR-GS(2017)

Subjects
0106 biological sciences, [SDV.BIO]Life Sciences [q-bio]/Biotechnology, pigments, algae based bioproducts, Plant Science, Biology, 01 natural sciences, SB1-1110, lipids, 03 medical and health sciences, domestication, [SDV.BBM]Life Sciences [q-bio]/Biochemistry, Molecular Biology, Domestication, ComputingMilieux_MISCELLANEOUS, 030304 developmental biology, Grand Challenges, 0303 health sciences, Agroforestry, green chemistry, microalgae, Plant culture, [SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology, Biofuel, biofuel, Specialty Grand Challenge, 010606 plant biology & botany
Abstract

International audience

Published
2021

7. Biofuels from oleaginous algae Principles, industry sectors, challenges

Author

Marechal, Eric, LIPID, Physiologie cellulaire et végétale (LPCV), Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes (UGA)-Institut de Recherche Interdisciplinaire de Grenoble (IRIG), Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes (UGA)-Institut de Recherche Interdisciplinaire de Grenoble (IRIG), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Commissariat à l’Energie Atomique et aux Energies Alternatives (programme Fermeture du Cycle du Carbone), Institut Carnot 3BCAR, ANR-11-BTBR-0008,OCEANOMICS,Biotechnologies et bioressources pour la valorisation des écosystèmes marins planctoniques(2011), ANR-10-LABX-0004,CeMEB,Labex(2010), ANR-17-EURE-0003,CBH-EUR-GS,CBH-EUR-GS(2017), Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche Interdisciplinaire de Grenoble (IRIG), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Université Grenoble Alpes (UGA)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche Interdisciplinaire de Grenoble (IRIG), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Université Grenoble Alpes (UGA), and ANR-10-LABX-0004,CeMEB,Mediterranean Center for Environment and Biodiversity(2010)

Subjects
[SDV.EE]Life Sciences [q-bio]/Ecology, environment, [SDV.BIO]Life Sciences [q-bio]/Biotechnology
Abstract

International audience; (~500-800 signes) : Résumé du texte-Résumé du texte Certains organismes photosynthétiques sont capables de capturer le CO2 atmosphérique et de produire une biomasse riche en huile. Cette huile est considérée de ce fait comme une ressource renouvelable, qui pourrait devenir une alternative aux hydrocarbures fossiles. Cet article fournit une définition détaillée de ce qu'on entend par microalgue, huile, biocarburant, et donne un état de l'art des technologies de culture, de récolte, d'extraction d'huile et de conversion en biodiesel, du laboratoire à l'échelle pilote, soulignant les verrous biotechnologiques et technologiques à lever dans l'avenir.

Published
2021

8. Tuning the functional properties of lignocellulosic films by controlling the molecular and supramolecular structure of lignin

Author

Stéphanie Baumberger, Amel Majira, Laurence Foulon, Miguel Pernes, Elise Gerbin, Monika Österberg, Carlos Marcuello, Bernard Kurek, A Gainvors-Claisse, David Crônier, Blandine Godon, Yves-Michel Frapart, Betty Cottyn, Véronique Aguié-Béghin, Guillaume Riviere, Université de Reims Champagne-Ardenne, Bioproduct Chemistry, Université de Paris, Université Paris-Saclay, Department of Bioproducts and Biosystems, Aalto-yliopisto, Aalto University, Fractionnement des AgroRessources et Environnement (FARE), Université de Reims Champagne-Ardenne (URCA)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Aalto University School of Science and Technology [Aalto, Finland], Laboratoire de Chimie et de Biochimie Pharmacologiques et Toxicologiques (LCBPT - UMR 8601), Centre National de la Recherche Scientifique (CNRS)-Université de Paris (UP), Institut Jean-Pierre Bourgin (IJPB), AgroParisTech-Université Paris-Saclay-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), European Project: 720303,Zelcor, Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Université Paris Cité (UPCité), H2020 BBI-JU, ANR Institut Carnot 3BCar, European FEDER Programme, and Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Université de Paris (UP)

Subjects
[SDV.BIO]Life Sciences [q-bio]/Biotechnology, [SDV]Life Sciences [q-bio], Composite number, 02 engineering and technology, Microscopy, Atomic Force, Biochemistry, Oligomer, Lignin, Antioxidants, law.invention, [SPI.MAT]Engineering Sciences [physics]/Materials, chemistry.chemical_compound, Structural Biology, law, Spectroscopy, Fourier Transform Infrared, Electron paramagnetic resonance, 0303 health sciences, Antioxidant and antibacterial properties, General Medicine, Free Radical Scavengers, Phenoxy radicals, 021001 nanoscience & nanotechnology, Anti-Bacterial Agents, Intercellular Signaling Peptides and Proteins, 0210 nano-technology, Staphylococcus aureus, Radical, Supramolecular chemistry, Microbial Sensitivity Tests, complex mixtures, Cellulose nanocomposite, Protobind 1000, 03 medical and health sciences, Phenols, Suspensions, Escherichia coli, Cellulose, Molecular Biology, 030304 developmental biology, technology, industry, and agriculture, Water, Colloid lignin particles (CLP), Biodegradable polymer, [CHIM.POLY]Chemical Sciences/Polymers, chemistry, Chemical engineering, Nanoparticles, Colloid lignin particles
Abstract

openaire: EC/H2020/720303/EU//Zelcor Funding Information: This work was funded by the Bio Based Industry Joint Undertaking under the European Union's Horizon 2020 research and innovation programme within the Zelcor project (under the grant number No 720303 ), part of the COFILI project (grant number D201550245 ) for AFM measurements funded by the Grand Est Region and the European FEDER Programme and the Lignoxyl project for EPR measurements supported by the Agence Nationale de la Recherche (ANR) through the Carnot Institutes 3BCAR ( www.3bcar.fr ) and Qualiment ( https://qualiment.fr/ ) (no. 3 no. 19-CARN-001-01 and no. 16-CARN 001-01). The EPR data in this manuscript were obtained using equipment supported jointly by the French National Ministry of Research (PPF IRPE), the “Fondation pour la Recherche Médicale” (FRM DGE20061007745), and the CNRS (Department of Chemistry and Life Sciences). The IJPB benefits from the support of the LabEx Saclay Plant Sciences-SPS (ANR-10-LABX-552 0040-SPS). Funding Information: This work was funded by the Bio Based Industry Joint Undertaking under the European Union's Horizon 2020 research and innovation programme within the Zelcor project (under the grant number No 720303), part of the COFILI project (grant number D201550245) for AFM measurements funded by the Grand Est Region and the European FEDER Programme and the Lignoxyl project for EPR measurements supported by the Agence Nationale de la Recherche (ANR) through the Carnot Institutes 3BCAR (www.3bcar.fr) and Qualiment (https://qualiment.fr/) (no. 3 no. 19-CARN-001-01 and no. 16-CARN 001-01). The EPR data in this manuscript were obtained using equipment supported jointly by the French National Ministry of Research (PPF IRPE), the ?Fondation pour la Recherche M?dicale? (FRM DGE20061007745), and the CNRS (Department of Chemistry and Life Sciences). The IJPB benefits from the support of the LabEx Saclay Plant Sciences-SPS (ANR-10-LABX-552 0040-SPS). Publisher Copyright: © 2021 The Authors Copyright: Copyright2021 Elsevier B.V., All rights reserved. This study investigated the relationships between lignin molecular and supramolecular structures and their functional properties within cellulose-based solid matrix, used as a model biodegradable polymer carrier. Two types of derivatives corresponding to distinct structuration levels were prepared from a single technical lignin sample (PB1000): phenol-enriched oligomer fractions and colloidal nanoparticles (CLP). The raw lignin and its derivatives were formulated with cellulose nanocrystals or nanofibrils to prepare films by chemical oxidation or pressure-assisted filtration. The films were tested for their water and lignin retention capacities, radical scavenging capacity (RSC) and antimicrobial properties. A structural investigation was performed by infrared, electron paramagnetic resonance spectroscopy and microscopy. The composite morphology and performance were controlled by both the composition and structuration level of lignin. Phenol-enriched oligomers were the compounds most likely to interact with cellulose, leading to the smoothest film surface. Their RSC in film was 4- to 6-fold higher than that of the other samples. The organization in CLP led to the lowest RSC but showed capacity to trap and stabilize phenoxy radicals. All films were effective against S. aureus (gram negative) whatever the lignin structure. The results show the possibility to tune the performances of these composites by exploiting lignin multi-scale structure.

Published
2021

9. Characterization of the Bubblegum acyl-CoA synthetase of Microchloropsis gaditana

Author

Fabrice Rébeillé, Mariette Bedhomme, Juliette Jouhet, Laurent Poulet, Sébastien Leterme, Serge Crouzy, Séverine Collin, Renaud Dumas, Frédéric Laueffer, Eric Maréchal, Alberto Amato, Laurent Fourage, Morgane Michaud, Elodie Billey, Leonardo Magneschi, Amélie Andres-Robin, Giovanni Finazzi, LIPID, Physiologie cellulaire et végétale (LPCV), Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche Interdisciplinaire de Grenoble (IRIG), Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Université Grenoble Alpes (UGA)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche Interdisciplinaire de Grenoble (IRIG), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Université Grenoble Alpes (UGA), Total Raffinage-Chimie, Light Photosynthesis & Metabolism (Photosynthesis), Régulateurs du développement de la fleur (Flo_RE ), Chimie computationnelle, modélisation et rayons X (COMX), Laboratoire de Chimie et Biologie des Métaux (LCBM - UMR 5249), Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche Interdisciplinaire de Grenoble (IRIG), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Grenoble Alpes (UGA)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche Interdisciplinaire de Grenoble (IRIG), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Grenoble Alpes (UGA), CEA-Total partnership, Conseil Regional Auvergne-Rhône-Alpes, the European Commission, and Institut Carnot 3BCAR (LIPANG platform), ANR-11-BTBR-0008,OCEANOMICS,Biotechnologies et bioressources pour la valorisation des écosystèmes marins planctoniques(2011), ANR-10-LABX-0049,GRAL,Grenoble Alliance for Integrated Structural Cell Biology(2010), and ANR-17-EURE-0003,CBH-EUR-GS,CBH-EUR-GS(2017)

Subjects
0106 biological sciences, eicosapentaenoic acid, Regular Issue, very-long-chain polyunsaturated fatty acid, Physiology, lipidosin, Plant Science, Cyanobacteria, 01 natural sciences, 03 medical and health sciences, chemistry.chemical_compound, Biosynthesis, Phosphatidylcholine, Coenzyme A Ligases, Genetics, Bubblegum Acyl-CoA synthase, Nannochloropsis, Photosynthesis, Plastid, 030304 developmental biology, 0303 health sciences, omega pathway, biology, [SDV.BBM.BS]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Structural Biology [q-bio.BM], Point mutation, Fatty Acids, [SDV.BV.BOT]Life Sciences [q-bio]/Vegetal Biology/Botanics, biology.organism_classification, Chloroplast, Cytosol, Metabolic pathway, chemistry, Biochemistry, lipids (amino acids, peptides, and proteins), Microchloropsis, Metabolic Networks and Pathways, 010606 plant biology & botany
Abstract

The metabolic pathways of glycerolipids are well described in cells containing chloroplasts limited by a two-membrane envelope but not in cells containing plastids limited by four membranes, including heterokonts. Fatty acids (FAs) produced in the plastid, palmitic and palmitoleic acids (16:0 and 16:1), are used in the cytosol for the synthesis of glycerolipids via various routes, requiring multiple acyl-Coenzyme A (CoA) synthetases (ACS). Here, we characterized an ACS of the Bubblegum subfamily in the photosynthetic eukaryote Microchloropsis gaditana, an oleaginous heterokont used for the production of lipids for multiple applications. Genome engineering with TALE-N allowed the generation of MgACSBG point mutations, but no knockout was obtained. Point mutations triggered an overall decrease of 16:1 in lipids, a specific increase of unsaturated 18-carbon acyls in phosphatidylcholine and decrease of 20-carbon acyls in the betaine lipid diacylglyceryl–trimethyl–homoserine. The profile of acyl-CoAs highlighted a decrease in 16:1-CoA and 18:3-CoA. Structural modeling supported that mutations affect accessibility of FA to the MgACSBG reaction site. Expression in yeast defective in acyl-CoA biosynthesis further confirmed that point mutations affect ACSBG activity. Altogether, this study supports a critical role of heterokont MgACSBG in the production of 16:1-CoA and 18:3-CoA. In M. gaditana mutants, the excess saturated and monounsaturated FAs were diverted to triacylglycerol, thus suggesting strategies to improve the oil content in this microalga.

Published
2021

10. DEVELOPPEMENT D'UN PROCEDE INTEGRE DE FERMENTATION ANAEROBIE COUPLEE A LA PERVAPORATION POUR LA PRODUCTION DE N-BUTANOL

Author

Moussa, Marwen, Auguste-Dormeuil, Charles, Galindo Alvarez, Lizeth, Pinault, Mailys, Athès-Dutour, Violaine, Meynial Salles, Isabelle, Génie et Microbiologie des Procédés Alimentaires (GMPA), Institut National de la Recherche Agronomique (INRA)-AgroParisTech, Laboratoire d'Ingénierie des Systèmes Biologiques et des Procédés (LISBP), Institut National de la Recherche Agronomique (INRA)-Institut National des Sciences Appliquées - Toulouse (INSA Toulouse), Institut National des Sciences Appliquées (INSA)-Université de Toulouse (UT)-Institut National des Sciences Appliquées (INSA)-Université de Toulouse (UT)-Centre National de la Recherche Scientifique (CNRS), Institut Carnot 3BCAR, and Projet BUTEXT

Subjects
Evolution in vivo, bioréacteur à membrane, [SPI]Engineering Sciences [physics], pervaporation, [SDV]Life Sciences [q-bio], n-butanol, procédé intégré
Abstract

International audience; pas de résumé

Published
2017

11. DEVELOPPEMENT D'UN PROCEDE INTEGRE DE FERMENTATION ANAEROBIE COUPLEE A LA PERVAPORATION POUR LA PRODUCTION DE N-BUTANOL

Author

MOUSSA, Marwen, Auguste-Dormeuil, Charles, Galindo Alvarez, Lizeth, Pinault, Mailys, Athès-Dutour, Violaine, Meynial Salles, Isabelle, Génie et Microbiologie des Procédés Alimentaires (GMPA), Institut National de la Recherche Agronomique (INRA)-AgroParisTech, Laboratoire d'Ingénierie des Systèmes Biologiques et des Procédés (LISBP), Institut National de la Recherche Agronomique (INRA)-Institut National des Sciences Appliquées - Toulouse (INSA Toulouse), Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Centre National de la Recherche Scientifique (CNRS), AgroParisTech, Institut Carnot 3BCAR, Projet BUTEXT, Centre National de la Recherche Scientifique (CNRS)-Institut National des Sciences Appliquées - Toulouse (INSA Toulouse), and Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Institut National de la Recherche Agronomique (INRA)

Subjects
bioréacteur à membrane, Evolution in vivo, [SPI]Engineering Sciences [physics], pervaporation, [SDV]Life Sciences [q-bio], n-butanol, ComputingMilieux_MISCELLANEOUS, procédé intégré
Abstract

International audience

Published
2017

12. Pervaporative fermentation for continuous anaerobic production of n-butanol with high titer, yield and productivity

Author

MOUSSA, Marwen, Galindo Alvarez, Lizeth, Pinault, Mailys, Meynial Salles, Isabelle, Athes-Dutour, Violaine, Génie et Microbiologie des Procédés Alimentaires (GMPA), Institut National de la Recherche Agronomique (INRA)-AgroParisTech, Laboratoire d'Ingénierie des Systèmes Biologiques et des Procédés (LISBP), Institut National de la Recherche Agronomique (INRA)-Institut National des Sciences Appliquées - Toulouse (INSA Toulouse), Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Centre National de la Recherche Scientifique (CNRS), Institut Carnot 3BCAR, BUTEXT, Institut National des Sciences Appliquées (INSA)-Université de Toulouse (UT)-Institut National des Sciences Appliquées (INSA)-Université de Toulouse (UT)-Centre National de la Recherche Scientifique (CNRS), Centre National de la Recherche Scientifique (CNRS)-Institut National des Sciences Appliquées - Toulouse (INSA Toulouse), and Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Institut National de la Recherche Agronomique (INRA)

Subjects
in vivo evolution, [SPI]Engineering Sciences [physics], pervaporation, [SDV]Life Sciences [q-bio], n-butanol, membrane bioreactor, integrated process
Abstract

International audience; Within the framework of the global climate change and decline of natural resources, the substitution of petrochemical products by biobased products is drawing an increasing attention. Butanol has a major interest due to its multifunctional characters and considerable possibilities on various applications sectors: energy, cosmetic and pharmaceutical formulations, chemistry, etc. In this context, it is widely believed that biotechnology will provide a more sustainable route to produce butanol from renewable biomass-derived feedstocks. Nevertheless, to develop robust biotechnological processes for full scale industrial implementation, it is necessary to resolve technical issues, such as product inhibition of butanol producing microorganisms. One way to tackle product inhibition and thus increase productivity and the overall sustainability of the process is the implementation of an " In Situ Product Recovery " (ISPR) using pervaporation [1]. Pervaporation represents a promising process for the extraction of bio-butanol. This membrane-based process has several advantages over the existing processes, mainly with regard to energy consumption. Further considerable advantages of this process are related to its compactness and ability to operate at variable scales while being easily integrated with fermentation. In hydrophobic pervaporation, organic compounds like n-butanol undergo a selective permeation through the membrane [2]. The liquid bulk (feed) is in direct contact with the organo-selective upstream side of the membrane while a butanol-enriched vapor phase (permeate) is removed from the opposite side. Even though the Acetone-Butanol-Ethanol (ABE) production by C. acetobulylicum has been developed at the industrial scale (Weizmann Process), some limitations are remaining, such as i) the co-production of acetone, ii) the low growth rate of the producing cells and their possible degeneration during a continuous process, iii) the complexity of the downstream process and its high environmental footprint. Recently, an engineered Escherichia coli strain was successfully developed by our group to produce n-butanol as the main metabolite obtained from glucose, with a high yield (0.28 g/g, i.e. 73% of the theoretical yield) [3]. The present work was aimed to develop an integrated process for the continuous production of n-butanol using the E. coli strain in a membrane bioreactor, coupled to a pervaporation unit, according the following strategy:  In vivo evolution of the E. coli strain: this step allowed selecting highly solvent-resistant mutants.  The implementation of the obtained mutants using a membrane bioreactor: this step allowed to increase the n-butanol production rate (+50%) and the glucose consumption rate (+85%)  A comparative study of various organo-selective pervaporation membranes for the extraction of bio-butanol from model media (pure compounds and in binary or ternary mixtures) and real media (resulting from fermentation): this step allowed to highlight the role of competitive effects and facilitated transfer between the components, as well as the effect of the medium complexity on transport mechanisms.  The use of the best performing membranes for the in situ recovery of n-butanol. This work brings new insights towards the implementation of a robust and intensified biotechnological process for the continuous production of n-butanol with high titer, yield and productivity. The work strategy is valuable for similar integrated bioprocesses. References [1] Van Hecke W, Kaur G,, De Wever H. (2014) Advances in in-situ product recovery (ISPR) in whole cell biotechnology during the last decade. Biotechnol Adv. 32:1245-55. [2] Vane L. (2008) Separation technologies for the recovery and dehydration of alcohols from fermentation broths. Biofuels, Bioprod. Bioref. 2:553–588 [3] Soucaille P, Meynial-Salles I, Foulquier C, Rivière A. (2015) New polypeptide having ferredoxin NADP+ reductase activity polynucleotide encoding the sale and uses thereof, PCT INSA/INRA/CNRS EP15306225 27 juillet.

Published
2017

13. A decision support system using multi-source scientific data, an ontological approach and soft computing - application to eco-efficient biorefinery

Author

Busset Guillaume, Dervaux Stephane, Dibie Juliette, Sablayrolles Caroline, Lousteau-Cazalet Charlotte, Belaud Jean-Pierre, Charnomordic Brigitte, Buche Patrice, Vialle Claire, Barakat Abdellatif, Destercke Sebastien, Ingénierie des Agro-polymères et Technologies Émergentes (UMR IATE), Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro)-Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Université Montpellier 2 - Sciences et Techniques (UM2)-Université de Montpellier (UM)-Institut National de la Recherche Agronomique (INRA), Laboratoire de génie chimique [ancien site de Basso-Cambo] (LGC), Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Centre National de la Recherche Scientifique (CNRS)-Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées, Graphs for Inferences on Knowledge (GRAPHIK), Laboratoire d'Informatique de Robotique et de Microélectronique de Montpellier (LIRMM), Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS)-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS)-Inria Sophia Antipolis - Méditerranée (CRISAM), Institut National de Recherche en Informatique et en Automatique (Inria)-Institut National de Recherche en Informatique et en Automatique (Inria), Mathématiques, Informatique et STatistique pour l'Environnement et l'Agronomie (MISTEA), Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro)-Institut National de la Recherche Agronomique (INRA), Mathématiques et Informatique Appliquées (MIA-Paris), AgroParisTech-Institut National de la Recherche Agronomique (INRA), Laboratoire d'Excellence 'Maîtrise des Systèmes de Systèmes Technologiques' (Labex MS2T), Université de Technologie de Compiègne (UTC)-Centre National de la Recherche Scientifique (CNRS), Heuristique et Diagnostic des Systèmes Complexes [Compiègne] (Heudiasyc), Chimie Agro-Industrielle (CAI), Institut National de la Recherche Agronomique (INRA)-Ecole nationale supérieure des ingénieurs en arts chimiques et technologiques-Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées, Laboratoire de Chimie Agro-Industrielle (LCA), Institut National de la Recherche Agronomique (INRA)-Ecole Nationale Supérieure de Chimie de Toulouse, Institut CARNOT 3BCAR, Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Institut National de la Recherche Agronomique (INRA)-Université Montpellier 2 - Sciences et Techniques (UM2)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Université de Montpellier (UM)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro), Centre National de la Recherche Scientifique (CNRS)-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS)-Université de Montpellier (UM)-Inria Sophia Antipolis - Méditerranée (CRISAM), Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut National de la Recherche Agronomique (INRA), Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Ecole nationale supérieure des ingénieurs en arts chimiques et technologiques-Institut National de la Recherche Agronomique (INRA), Université de Toulouse (UT)-Université de Toulouse (UT)-Centre National de la Recherche Scientifique (CNRS)-Institut National Polytechnique (Toulouse) (Toulouse INP), Université de Toulouse (UT), Institut National de la Recherche Agronomique (INRA)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), Institut National de la Recherche Agronomique (INRA)-AgroParisTech, Institut National de la Recherche Agronomique (INRA)-Ecole nationale supérieure des ingénieurs en arts chimiques et technologiques (ENSIACET), Université de Toulouse (UT)-Université de Toulouse (UT)-Institut National Polytechnique (Toulouse) (Toulouse INP), and Université de Toulouse (UT)-Université de Toulouse (UT)

Subjects
Reliability theory, Decision support system, knowledge, Computer science, 020209 energy, Context (language use), 02 engineering and technology, Scientific literature, Ontology (information science), bioprocess eco-design, Engineering, ontology, knowledge engineering, fuzzy numbers, belief theory, uncertainty management, 0202 electrical engineering, electronic engineering, information engineering, Fuzzy number, Semantic Web, Automatique, Soft computing, Ontology, [INFO.INFO-WB]Computer Science [cs]/Web, Automatic Control Engineering, Experimental data, Fuzzy numbers, Data science, Software quality, management
Abstract

International audience; In decision tasks such as bioprocess efficiency comparison, scientific literature is a valuable source of data. This large number of scientific data is heterogeneously structured, mainly in textual format. Innovative tools able to integrate and treat constantly new information are required. In this context, the use of semantic web methods such as ontologies seems relevant to structure the experimental information. Imprecision and uncertainty can arise from data incompleteness and variability. This is particularly true for processes involving biological materials. Document reliability should also be considered. Soft computing methods have the potential to be the kingpin of specialized software that can be integrated in decision support systems (DSS) intended to solve these issues. This paper presents the implementation of a pipeline which permits to: (1) structure and integrate the experimental data of interest by using ontologies, (2) assess data source reliability, (3) compute and visualize indicators taking into account data imprecision.

Published
2016

14. Comparison of various milling modes combined to the enzymatic hydrolysis of lignocellulosic biomass for bioenergy production: Glucose yield and energy efficiency

Author

Abderrahim Solhy, Florian Monlau, Abdellatif Barakat, A. Licari, Patrice Buche, Ingénierie des Agro-polymères et Technologies Émergentes (UMR IATE), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Institut National de la Recherche Agronomique (INRA)-Université Montpellier 2 - Sciences et Techniques (UM2)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Université de Montpellier (UM)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro), Center for Advanced Materials, Université Mohammed VI Polytechnique, Office Chérifien des Phosphates in the Moroccan Kingdom (OCP Group), and Institut Carnot 3BCAR (Bioénergies, Biomolécules et Biomatériaux du Carbone Renouvelable)

Subjects
Materials science, Glucose yield, 020209 energy, hydrolyse enzymatique, Biomass, Lignocellulosic biomass, 02 engineering and technology, 010501 environmental sciences, 01 natural sciences, 7. Clean energy, Industrial and Manufacturing Engineering, Bioenergy, Enzymatic hydrolysis, 0202 electrical engineering, electronic engineering, information engineering, [SPI.GPROC]Engineering Sciences [physics]/Chemical and Process Engineering, Electrical and Electronic Engineering, valorisation des sous produits, Ball mill, Milling, 0105 earth and related environmental sciences, Civil and Structural Engineering, Energy, Waste management, Mechanical Engineering, broyage mécanique, Jet mill, enzymatic hydrolysis, Building and Construction, pretreatment, Pulp and paper industry, Pollution, efficience énergétique, General Energy, Energy efficiency, fibre lignocellulosique, Biofuel, bagasse, prétraitement, Bagasse
Abstract

Bagasse is an abundant by-product from sugarcane production that can be used for conversion into biofuels. Nonetheless, the recalcitrant structures of lignocellulosic fibers required a pretreatment prior conversion into biofuels. In this study, four mechanical deconstruction methods were compared in terms of energy demand and energy efficiency at lab scale: BM (ball mill), VBM (vibratory ball mill), CM (centrifugal mill) and JM (jet mill). Results indicate that VBM was more effective compared to BM, JM and CM in enzymatic accessibility and sugars solubilization: VBM-3h > BM-72 h > JM-5000 rpm > CM-0.12 mm. However, preliminary energetic assessment showed that at lab scale, the CM (centrifugal mill) as mechanical fractionation process appears to be the most efficient in terms of energy-efficiency (kg glucose/kWh) compared to BM, VBM and JM. A comparison with literature pretreatments data highlighted that fine and/or ultrafine milling process (BM, VBM, CM) are simpler saccharification technologies, which not required any chemical or water inputs, thus minimizing waste generation and treatment.

Published
2016

15. L’écotoxicologie à l’INRA

Author

Mougin, Christian, Physicochimie et Ecotoxicologie des SolS d'Agrosystèmes Contaminés (PESSAC), Institut National de la Recherche Agronomique (INRA), and Institut Carnot 3BCAR

Subjects
[SDV]Life Sciences [q-bio], [SDE]Environmental Sciences, ComputingMilieux_MISCELLANEOUS
Abstract

National audience

Published
2014

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