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450 results on '"Inoshima, Yasuo"'

2. First Report of Mermithidae (Enoplea: Mermithida) Parasitizing Adult Stable Flies in Japan

Author

Shimizu Kaori, Saito Taizo, Takashima Yasuhiro, Okada Haruhiko, Asakawa Mitsuhiko, and Inoshima Yasuo

Subjects
blood-feeding pests, genetics, host–parasite relationship, insect parasitism, mermithidae, stable fly, Biology (General), QH301-705.5
Abstract

Mermithidae is a family of nematodes that parasitize a wide range of invertebrates worldwide. Herein, we report nematodes that were unexpectedly found in three of 486 adult stable flies (Stomoxys calcitrans) captured from three farms (F1, F2, and F3) in different regions of Gifu Prefecture, Japan. We aimed to characterize these nematodes both at the morphological and molecular level. Morphological studies revealed that the nematodes were juveniles of Mermithidae. Phylogenetic analysis based on 18S and 28S rDNA indicated that the mermithids from farms F1 and F2 could be categorized into the same cluster as Ovomermis sinensis and Hexamermis sp., whereas the mermithid from farm F3 clustered with Amphimermis sp. Additionally, these mermithids could be categorized within the same clusters as related mermithids detected in Japan that parasitize various arthropod orders. Our findings suggest that these stable flies may have been parasitized by mermithids already present in the region and that genetically distinct species of mermithids occur across Japan. To the best of our knowledge, this is the first report of mermithids parasitizing adult stable flies in Japan.

Published
2024
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5. A new enzyme-linked immunosorbent assay for serological diagnosis of seal parapoxvirus infection in marine mammals

Author

Badr Yassien, Rahman Md. Matiur, Ohno Yoshito, Ishijima Keita, Maeda Ken, Kohyama Kaoru, Kamatari Yuji O., Shimizu Kaori, Okada Ayaka, and Inoshima Yasuo

Subjects
aquarium, elisa, parapoxvirus, pinnipeds, western blotting, Veterinary medicine, SF600-1100
Abstract

Seal parapoxvirus (SPPV) infection has been reported among pinnipeds in aquaria in Japan; however, its seroprevalence is unknown. Therefore, an enzyme-linked immunosorbent assay (ELISA) was developed for serological diagnosis of SPPV infection.

Published
2022
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18. Transmission of extended-spectrum cephalosporin-resistant Salmonella harboring a blaCMY-2-carrying IncA/C2 plasmid chromosomally integrated by ISEcp1 or IS26 in layer breeding chains in Japan

Author

SHIGEMURA, Hiroaki, MAEDA, Takashi, NAKAYAMA, Shiko, OHISHI, Akira, CARLE, Yuki, OOKUMA, Eiko, ETOH, Yoshiki, HIRAI, Shinichiro, MATSUI, Mari, KIMURA, Hirokazu, SEKIZUKA, Tsuyoshi, KURODA, Makoto, SERA, Nobuyuki, INOSHIMA, Yasuo, and MURAKAMI, Koichi

Subjects
Full Paper, IS26, layer breeding chain, Salmonella enterica, Bacteriology, beta-Lactamases, Anti-Bacterial Agents, Cephalosporins, bla CMY-2, Japan, Salmonella, Drug Resistance, Multiple, Bacterial, Animals, chromosomally-integrated IncA/C2 plasmid, Female, Chickens, Plasmids
Abstract

Dissemination of extended-spectrum cephalosporin (ESC)-resistant Salmonella is a public health concern in the egg production industry. ESC-resistant Salmonella often acquires the bla gene via insertion sequences (ISs). Therefore, this study aimed to assess antimicrobial resistance in Salmonella from Japanese layer breeding chains and egg processing chains, and determine the genetic profiles of IS-like elements in ESC-resistant Salmonella. Antimicrobial susceptibility testing was performed on 224 isolates from 49 facilities involving layer breeder farms, hatcheries, pullet-rearing farms, and layer farms in breeding chains along with egg processing chains. ESC-resistant Salmonella strains were whole-genome sequenced. Among them, 40 (17.9%) were resistant to at least streptomycin, tetracycline, ampicillin, chloramphenicol, cefpodoxime, nalidixic acid, ciprofloxacin, and/or kanamycin despite lacking resistance to azithromycin and meropenem. Moreover, 15 were ESC-resistant Salmonella harboring blaCMY-2 (Salmonella enterica serovar Ohio, n=12; S. Braenderup, n=1; untypeable with O7:b:-, n=1) and blaCTX-M-14 (S. Cerro, n=1). IncA/C2 plasmids containing ISEcp1, IS26, and multiple antimicrobial resistance genes (including blaCMY-2) were identified in S. Ohio isolates from pullet-rearing and layer farms belonging to the same company. Chromosomal integration of partial or whole IncA/C2 plasmids was seen with two S. Ohio isolates via ISEcp1 or IS26, respectively. Antimicrobial resistance genes such as blaCMY-2 might be transmitted among the upper and the lower levels of layer breeding chains via the replicon type IncA/C2 plasmids containing ISEcp1 and IS26.

Published
2021

19. Longitudinal study of northern fur seal (Callorhinus ursinus) hematology

Author

KOHYAMA, Kaoru, KIYOTA, Masashi, and INOSHIMA, Yasuo

Subjects
Erythrocyte Indices, northern fur seal, Full Paper, hematology, Fur Seals, Animals, Wildlife Science, Animals, Wild, erythrocyte, Longitudinal Studies, leukocyte, seasonal change
Abstract

Although the causes have not been specified yet, wild populations of northern fur seals (Callorhinus ursinus) have been decreasing, which is why conservation techniques such as captive breeding and health maintenance should be established. Hematological parameters can be used to maintain the health status of northern fur seals kept in captivity for artificial reproduction and public education. Year-round fluctuations of blood parameters have not been examined for northern fur seals due to the difficulties in obtaining serial blood samples from wild animals during the oceanic migration period from late autumn to spring. In this study, blood samples were collected from four captive northern fur seals and analyzed monthly for more than three years to clarify the seasonal fluctuation patterns in 14 hematological parameters. Many hematological parameters seemed to be seasonal patterns: summer-autumn and winter-spring; leukocyte-related parameters were higher in summer and autumn than in winter and spring; erythrocyte-related parameters were lower in summer and autumn than in winter and spring. Significant seasonal differences in nine of the 14 parameters were observed using a generalized linear mixed model (GLMM) analysis. These results have improved our understanding of the seasonal patterns of hematological characteristics in the northern fur seal and can contribute to the health care of protected or captive northern fur seals.

Published
2021

34. Leukopenia induced by micafungin in a bottlenose dolphin (Tursiops truncatus): a case report

Author

OHNO, Yoshito, AKUNE, Yuichiro, NITTO, Hiroshi, and INOSHIMA, Yasuo

Subjects
Bottle-Nosed Dolphin, bottlenose dolphin, leukopenia, micafungin, Animals, Female, Wildlife Science, fungal pneumonia, Pulmonary Aspergillosis, Pneumonia, G-CSF, Note
Abstract

A bottlenose dolphin (Tursiops truncatus) housed in the Port of Nagoya Public Aquarium (PNPA) presented with symptomatic pneumonia caused by Aspergillus fumigatus. The dolphin was treated with micafungin. On days 2 and 11 after the first administration of micafungin, results from a physical examination and laboratory test indicated a decline of body temperature (BT) and leukopenia, with lowest BT, white blood cells (WBCs), and segmented neutrophils (SEGs) of 34.2°C, 600 cells/µl, and 67 cells/µl, respectively. BT, WBCs, and SEGs returned to normal range after administration of granulocyte colony stimulating factor (G-CSF). To the best of our knowledge, this is the first report of micafungin-induced decline of BT and leukopenia that was successfully treated with G-CSF in a bottlenose dolphin.

Published
2019

42. Metagenomic identification, sequencing, and genome analysis of porcine hepe-astroviruses (bastroviruses) in porcine feces in Japan

Author

Nagai, Makoto, primary, Okabayashi, Tamaki, additional, Akagami, Masataka, additional, Matsuu, Aya, additional, Fujimoto, Yoshikazu, additional, Hashem, Md Abul, additional, Mekata, Hirohisa, additional, Nakao, Ryo, additional, Matsuno, Keita, additional, Katayama, Yukie, additional, Oba, Mami, additional, Omatsu, Tsutomu, additional, Asai, Tetsuo, additional, Nakagawa, Keisuke, additional, Ito, Hiroshi, additional, Madarame, Hiroo, additional, Kawai, Kazuhiro, additional, Ito, Toshihiro, additional, Nonaka, Nariaki, additional, Tsukiyama-Kohara, Kyoko, additional, Inoshima, Yasuo, additional, Mizutani, Tetsuya, additional, and Misawa, Naoaki, additional

Published
2021
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44. Enzootic nasal tumor virus type 2 envelope of goats acts as a retroviral oncogene in cell transformation

Author

Maeda, Naoyoshi, Inoshima, Yasuo, De las Heras, Marcelo, Maenaka, Katsumi, Maeda, Naoyoshi, Inoshima, Yasuo, De las Heras, Marcelo, and Maenaka, Katsumi

Abstract

Enzootic nasal tumor virus type 1 (ENTV-1) (ovine nasal tumor virus) and ENTV-2 (caprine nasal tumor virus) are known to be causative agents of enzootic nasal adenocarcinoma (ENA) in sheep and goats, respectively. Although the nucleotide and amino acid sequences of ENTV-1 and ENTV-2 are quite similar, they are recognized as phylogenetically distinct viruses. The envelope protein of ENTV-1 functions as an oncoprotein in the in vitro transformation of epithelial cells and fibroblasts. Thus, it is the primary determinant of in vivo tumorigenesis in ENA. As per our knowledge, no previous studies have reported in detail the role of ENTV-2 in ENA tumorigenesis. Here, in order to investigate the molecular mechanism of caprine ENA oncogenesis by ENTV-2, we have attempted to identify the transforming potential of ENTV-2 envelope, and investigated the activation of cell signaling pathways in oncogenic transformation. Our findings confirmed that ENTV-2 envelope was capable of inducing oncogenic transformation of rat cell lines in vitro. Further, we found that MAPK, Akt, and p38 were constitutively activated in ENTV-2 envelope-transformed clone cells. In addition, inhibitor experiments revealed that MEK-MAPK and PI3K-Akt signaling pathways are involved in the ENTV-2 envelope-induced cell transformation. These data indicate that ENTV-2 envelope could induce oncogenic transformation by signaling pathways that are also utilized by ENTV-1 envelope.

Published
2020

45. Enzootic nasal tumor virus type 2 envelope of goats acts as a retroviral oncogene in cell transformation

Author

1000080444800, Maeda, Naoyoshi, Inoshima, Yasuo, De las Heras, Marcelo, 1000010322752, Maenaka, Katsumi, 1000080444800, Maeda, Naoyoshi, Inoshima, Yasuo, De las Heras, Marcelo, 1000010322752, and Maenaka, Katsumi

Abstract

Enzootic nasal tumor virus type 1 (ENTV-1) (ovine nasal tumor virus) and ENTV-2 (caprine nasal tumor virus) are known to be causative agents of enzootic nasal adenocarcinoma (ENA) in sheep and goats, respectively. Although the nucleotide and amino acid sequences of ENTV-1 and ENTV-2 are quite similar, they are recognized as phylogenetically distinct viruses. The envelope protein of ENTV-1 functions as an oncoprotein in the in vitro transformation of epithelial cells and fibroblasts. Thus, it is the primary determinant of in vivo tumorigenesis in ENA. As per our knowledge, no previous studies have reported in detail the role of ENTV-2 in ENA tumorigenesis. Here, in order to investigate the molecular mechanism of caprine ENA oncogenesis by ENTV-2, we have attempted to identify the transforming potential of ENTV-2 envelope, and investigated the activation of cell signaling pathways in oncogenic transformation. Our findings confirmed that ENTV-2 envelope was capable of inducing oncogenic transformation of rat cell lines in vitro. Further, we found that MAPK, Akt, and p38 were constitutively activated in ENTV-2 envelope-transformed clone cells. In addition, inhibitor experiments revealed that MEK-MAPK and PI3K-Akt signaling pathways are involved in the ENTV-2 envelope-induced cell transformation. These data indicate that ENTV-2 envelope could induce oncogenic transformation by signaling pathways that are also utilized by ENTV-1 envelope.

Published
2020

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