1. Evaluation of the optical and biomechanical properties of bioengineered human skin generated with fibrin-agarose biomaterials
- Author
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Ana Maria Ionescu, Jesús Chato-Astrain, Ingrid Garzón Bello, Fernando Campos, María del Mar Pérez Gómez, Miguel Alaminos, and Juan de la Cruz Cardona Pérez
- Subjects
Paper ,optical properties ,Materials science ,Biomedical Engineering ,Biocompatible Materials ,Human skin ,fibrin-agarose biomaterial ,Absorption (skin) ,01 natural sciences ,Fibrin ,Absorption ,010309 optics ,Biomaterials ,Bioengineered skin ,Scattering ,chemistry.chemical_compound ,0103 physical sciences ,Skin substitutes ,Humans ,General ,Skin ,Physical point ,Tissue Engineering ,integumentary system ,biology ,Optical properties ,Sepharose ,scattering ,Atomic and Molecular Physics, and Optics ,Electronic, Optical and Magnetic Materials ,Fibrin-agarose biomaterial ,Tissue optics ,chemistry ,bioengineered skin ,biology.protein ,Agarose ,absorption ,Biomedical engineering - Abstract
Significance: Recent generation of bioengineered human skin allowed the efficient treatment of patients with severe skin defects. However, the optical and biomechanical properties of these models are not known. Aim: Three models of bioengineered human skin based on fibrin-agarose biomaterials (acellular, dermal skin substitutes, and complete dermoepidermal skin substitutes) were generated and analyzed. Approach: Optical and biomechanical properties of these artificial human skin substitutes were investigated using the inverse adding-doubling method and tensile tests, respectively. Results: The analysis of the optical properties revealed that the model that most resembled the optical behavior of the native human skin in terms of absorption and scattering properties was the dermoepidermal human skin substitutes after 7 to 14 days in culture. The time-course evaluation of the biomechanical parameters showed that the dermoepidermal substitutes displayed significant higher values than acellular and dermal skin substitutes for all parameters analyzed and did not differ from the control skin for traction deformation, stress, and strain at fracture break. Conclusions: We demonstrate the crucial role of the cells from a physical point of view, confirming that a bioengineered dermoepidermal human skin substitute based on fibrin-agarose biomaterials is able to fulfill the minimal requirements for skin transplants for future clinical use at early stages of in vitro development., Ministry of Science, Innovation and Universities of Spain PGC2018-101904-A-I0, Instituto de Salud Carlos III (ISCIII), Ministry of Science, Innovation and Universities, through AES 2017 AC17/00013, Instituto de Salud Carlos III (ISCIII), Ministry of Science, Innovation and Universities within EuroNanoMed framework, EU AC17/00013, University of Granada A.TEP.280.UGR18, Junta de Andalucía PE-0395-2019, Fundación Benéfica Anticancer San Francisco Javier y Santa Cándida, Granada, Spain, OTRI.35A-07
- Published
- 2020
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