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13 results on '"Infectious microorganisms"'

1. From the History of the Infection Study

Author

L. M. Farkhutdinova

Subjects
infectious theory, infectious epidemics, theory of miasms, infectious microorganisms, Internal medicine, RC31-1245
Abstract

The article reflects the development of ideas about infection, from the ancient period to the present day. In the V century Hippocrates proposed a miasmatic theory, according to which diseases are caused by harmful fumes. This paradigm remained dominant for 2.5 millennia. Although the existence of microorganisms has been known since 1676, when they were first described by Anthony van Leeuwenhoek, for a long time the detection of microbes in the biosubstrates of a sick person was considered as a secondary phenomenon in relation to the disease. The theoretical basis for such ideas was the concept of spontaneous generation, which has dominated since the time of Aristotle. The change from the miasmatic theory to the infectious paradigm was due to the fundamental discoveries of Louis Pasteur, who proved the biological nature of fermentation and the infectious genesis of silkworm diseases. The listed discoveries put an end to the discussion about spontaneous generation, became the scientific justification for asepsis and antiseptics and aimed at searching for pathogens of infectious human diseases, which led to a surge in discoveries in microbiology. The causative agents of fever (1868), leprosy (1873), anthrax (1876), tuberculosis (1882), cholera (1883), diphtheria (1884), plague (1894), etc. were discovered. As a result, the infectious theory finally conquered the world. An important achievement of the late 19th century was the allocation of a new type of infectious agents — viruses, which make up the most numerous form of life. With the recognition of the infectious theory at the end of the 19th century an active search for antimicrobial agents began. In 1943, the mass production of the first antibiotic, penicillin, was launched, the discovery of which is called one of the most outstanding achievements in the history of mankind. The use of antimicrobial drugs, along with mass vaccination, led to a significant decrease in the share of infectious diseases in the structure of mortality.

Published
2021
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2. Bacteriological Surveillance of Ambulance Vehicles from a Tertiary Care Hospital of North India

Author

Shiwani Sharma, Priya Datta, Varsha Gupta, Dipanshu Vasesi, and Jagdish Chander

Subjects
ambulance, disinfection, infectious microorganisms, multidrug resistant, Medicine
Abstract

Objective An ambulance is a medically equipped vehicle which is used in case of any medical emergency for the transport of patients to treatment facilities. The ambulances help in the transportation of thousands of patients per year, and such patients may carry infectious microorganisms which pose a major threat to the treatment of such patients. In this study, we analyzed the extent of bacterial contamination in our ambulance vehicles and measured the degree of antimicrobial resistance among isolated pathogens. Material and Method This study included five ambulances of our tertiary care hospital and different random sites were swabbed in each vehicle. These were selected based on their well-known high frequency of contact by emergency personnel and patients. Swabs were inserted into sterile test tubes containing normal saline and immediately transferred to our microbiology laboratory to identify bacterial contaminants utilizing standard microbiological procedures. Result A total of 198 swab samples were collected from all the five ambulances, out of which 170 (85.8%) swabs were sterile and 28 (14.2%) swabs yielded potentially pathogenic bacterial isolates. The highest contamination rate with pathogenic bacteria was detected in the oxygen flow meter knob (60%), suction machine tubing (60%), and stethoscope (40%). Staphylococcus aureus (32%) was the most frequently detected microorganism. Conclusion Our study showed low prevalence of bacterial contamination in ambulances because of good infection control policy of our hospital, however, some areas still need improvement and require proper standard operating procedures of disinfection policies of these emergency vehicles.

Published
2021
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3. Bacteriological Surveillance of Ambulance Vehicles from a Tertiary Care Hospital of North India.

Author

Sharma, Shiwani, Datta, Priya, Gupta, Varsha, Vasesi, Dipanshu, and Chander, Jagdish

Subjects
AMBULANCES, TERTIARY care, EMERGENCY vehicles, BACTERIAL contamination, TRANSPORTATION of patients
Abstract

Objective An ambulance is a medically equipped vehicle which is used in case of any medical emergency for the transport of patients to treatment facilities. The ambulances help in the transportation of thousands of patients per year, and such patients may carry infectious microorganisms which pose a major threat to the treatment of such patients. In this study, we analyzed the extent of bacterial contamination in our ambulance vehicles and measured the degree of antimicrobial resistance among isolated pathogens. Material and Method This study included five ambulances of our tertiary care hospital and different random sites were swabbed in each vehicle. These were selected based on their well-known high frequency of contact by emergency personnel and patients. Swabs were inserted into sterile test tubes containing normal saline and immediately transferred to our microbiology laboratory to identify bacterial contaminants utilizing standard microbiological procedures. Result A total of 198 swab samples were collected from all the five ambulances, out of which 170 (85.8%) swabs were sterile and 28 (14.2%) swabs yielded potentially pathogenic bacterial isolates. The highest contamination rate with pathogenic bacteria was detected in the oxygen flow meter knob (60%), suction machine tubing (60%), and stethoscope (40%). Staphylococcus aureus (32%) was the most frequently detected microorganism. Conclusion Our study showed low prevalence of bacterial contamination in ambulances because of good infection control policy of our hospital, however, some areas still need improvement and require proper standard operating procedures of disinfection policies of these emergency vehicles. [ABSTRACT FROM AUTHOR]

Published
2021
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4. Point-of-care microfluidic devices for pathogen detection.

Author

Nasseri, Behzad, Soleimani, Neda, Rabiee, Navid, Kalbasi, Alireza, Karimi, Mahdi, and Hamblin, Michael R.

Subjects
*MICROFLUIDIC devices, *POINT-of-care testing, *CELL culture, *MICROREACTORS, *ENERGY consumption, *SINGLE molecules
Abstract

The rapid diagnosis of pathogens is crucial in the early stages of treatment of diseases where the choice of the correct drug can be critical. Although conventional cell culture-based techniques have been widely utilized in clinical applications, newly introduced optical-based, microfluidic chips are becoming attractive. The advantages of the novel methods compared to the conventional techniques comprise more rapid diagnosis, lower consumption of patient sample and valuable reagents, easy application, and high reproducibility in the detection of pathogens. The miniaturized channels used in microfluidic systems simulate interactions between cells and reagents in microchannel structures, and evaluate the interactions between biological moieties to enable diagnosis of microorganisms. The overarching goal of this review is to provide a summary of the development of microfluidic biochips and to comprehensively discuss different applications of microfluidic biochips in the detection of pathogens. New types of microfluidic systems and novel techniques for viral pathogen detection (e.g. HIV, HVB, ZIKV) are covered. Next generation techniques relying on high sensitivity, specificity, lower consumption of precious reagents, suggest that rapid generation of results can be achieved via optical based detection of bacterial cells. The introduction of smartphones to replace microscope based observation has substantially improved cell detection, and allows facile data processing and transfer for presentation purposes. [ABSTRACT FROM AUTHOR]

Published
2018
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5. Airborne Infectious Microorganisms☆

Author

Gonzalez-Martin, Cristina

Subjects
SARS, Severe acute respiratory syndrome, WMD, weapons of mass destruction, Airborne infectious viruses, Enteric viruses, MERS, Middle East Respiratory Syndrome, Avian influenza, Varicella, Article, Blastomycosis, Nocardia, Coccidiomycosis, Hantaviruses, MERS-CoV, MERS – Associated coronavirus, Airborne infectious bacteria, Aspergillosis, Tuberculosis, Airborne infectious fungi, Histoplasmosis, Bioaerosol, fungi, Cryptococcosis, Infectious microorganisms, Bioterrorism, Influenza, Severe acute respiratory syndrome, Norwalk-like viruses, HPS, Hantavirus pulmonary syndrome, HVAC, Heating, ventilation, and air conditioning, SARS-CoV, SARS-associated Coronavirus, Legionnaires’ disease, Measles, Smallpox
Abstract

Inhalation exposes the upper and lower respiratory tracts of humans to a variety of airborne particles and vapors. Airborne transmission of pathogenic microorganisms to humans from the environment, animals, or other humans can result in disease. Inhalation is an important route of exposure as the lung is more susceptible to infection than the gastrointestinal tract. Ingested microorganisms must pass through the acidic environment of the stomach before they can colonize tissue while inhaled microorganisms are deposited directly on the moist surfaces of the respiratory tract. Inhalation of microbial aerosols can elicit adverse human health effects including infection, allergic reaction, inflammation, and respiratory disease. Following inhalation, infectious viruses, bacteria, and fungi can establish in host cells of the respiratory tract. Some are translocated and infect the gastrointestinal tract and other tissues. This article discusses human viral, bacterial, and fungal diseases transmitted via aerosols. Viral diseases presented are influenza, severe acute respiratory syndrome (SARS), Middle East respiratory syndrome (MERS), enteric viruses related infections, hantavirus disease, measles, and varicella. Bacterial diseases are Legionnaires’ disease, tuberculosis, and nontubercule mycobacterial disease. Exposure to some Gram-negative and Gram-positive bacteria, endotoxin, and actinomycetes when dispersed through the air can result in disease following inhalation. Fungal diseases included are histoplasmosis, coccidiomycosis, blastomycosis, cryptococcosis, and aspergillosis. The threat of bioterrorism with airborne infectious agents is also briefly presented.

Published
2019

6. Size‐dependent antimicrobial response of zinc oxide nanoparticles.

Author

Palanikumar, Loganathan, Ramasamy, Sinna Nadar, and Balachandran, Chandrasekaran

Abstract

Antibacterial and antifungal activities of zinc oxide nanoparticles (ZnO NPs) were investigated against infectious microorganisms. ZnO NPs were prepared by wet chemical precipitation method varying the pH values. Particle size and morphology of the as‐prepared ZnO powders were characterised by X‐ray diffraction, Fourier transform infrared spectroscopy and transmission electron microscope. The zone of inhibition by NPs ranged from 0 to 17 mm. The lowest minimum inhibitory concentration value of NPs is 25 µg.ml−1 against Staphylococcus epidermidis. These studies demonstrate that ZnO NPs have wide range of antimicrobial activities towards various microorganisms. The results obtained in the authors' study indicate that the inhibitory efficacy of ZnO NPs is significantly dependent on its chosen concentration and size. Significant inhibition in antibacterial response was observed for S. epidermidis when compared with control antibiotic. [ABSTRACT FROM AUTHOR]

Published
2014
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7. BIOLOGICZNE CZYNNIKI ZAGROŻENIA ZAWODOWEGO -- AKTUALNE PROBLEMY.

Author

Dutkiewicz, Jacek

Subjects
CORONAVIRUSES, VETERINARIANS, SARS disease, NIPAH virus, ENCEPHALITIS, HANTAVIRUSES, HEMORRHAGIC fever, HEPATITIS C virus, AIDS, WORK environment, GRAM-negative bacteria, RESPIRATORY diseases, OCCUPATIONAL diseases
Abstract

Copyright of Medycyna Pracy is the property of Instytut Medycyny Pracy im. Jerzego Nofera and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Published
2004

8. Визначення параметрів електромагнітного випромінювання для енергоінформаційної дезінфекції вовни в процесі її первинної обробки

Author

Kosulina, Nataliaya, Cherenkov, Aleksandr, Pirotti, Evgenij, Moroz, Sergey, and Chorna, Mariya

Subjects
UDC 577.3, electromagnetic radiation of millimeter range, infectious microorganisms, biotropic parameters, wool disinfection, электромагнитное излучение миллиметрового диапазона, инфекционные микроорганизмы, биотропные параметры, дезинфекция шерсти, електромагнітне випромінювання міліметрового діапазону, інфекційні мікроорганізми, біотропні параметри, дезинфекція шерсті
Abstract

The paper is devoted to solution of theoretical and experimental problems in development of electromagnetic (EM) technology in the millimeter wavelength range for disinfection and warming of baled wool during its processing.Sheared wool is an environment well fit for existence of a variety of microorganisms. It was established that 1 kg of wool contains 400 to 7000 million bacteria. This often leads to workers infection with pathogens of brucellosis, myterosis, glanders, toxicoplasmosis, ku-reketmosis, leptospirosis, anthrax and plague.When storing unwashed wool, especially at low temperatures, its compression and fat hardening take place. Fat softens and the rune easily unfolds at temperatures of 25...30 °C. This improves sorting quality and preserves natural wool properties.In this regard, the work studied the regularities of influence of EM energy in the millimeter wavelength range on the pathogenic microorganisms existing in wool.As a result of the studies, a range of changes in the biotropic EMF parameters was determined for destruction of microorganisms. The performed calculations have shown that the biotropic parameters of electromagnetic field include frequency range of 35.3...36.3 GHz; power flux density of 1.25 mW/cm2, exposure time of 2·103 s.Theoretical analysis of the model of warming up baled wool have shown that the temperature rise up to 24...40 °C is possible when wool is exposed to EMF with the following parameters (frequency; power flux density; exposure time): 36 GHz; 1.25...1.5 mW/cm2; 120...180 s.It was established that when wool was exposed to an EMF with certain biotropic parameters, wool breaking load increased by 200...300 cN and relative strength increased by 2...3 cN/tex in comparison with the control runs. The study results make it possible to create an environmentally friendly and efficient technology for primary wool processing by EM energy., Угнетение инфекционных микроорганизмов в кипах шерсти возможно при помощи электромагнитного излучения (ЭМИ) с параметрами: частота 36 ГГц, мощность 0,5 кВт, экспозиция 3 мин. Энергоинформационные параметры были определены в результате теоретических и экспериментальных исследований процесса взаимодействия ЭМИ с инфекционными микроорганизмами, Пригнічення інфекційних мікроорганізмів у кіпах вовни можливо за допомогою електромагнітного випромінювання (ЕМВ) з параметрами: частота 36 ГГц, потужність 0,5 кВт, експозиція 3 хв. Енергоінформаційні параметри були визначені в результаті теоретичних і експериментальних досліджень процесу взаємодії ЕМВ з інфекційними мікроорганізмами

Published
2017

9. Determining parameters of electromagnetic radiation for energoinformational disinfection of wool in its pretreatment

Author

Kosulina, Nataliaya; Kharkiv Petro Vasylenko National Technical University of Agriculture Alchevskih str., 44, Kharkiv, Ukraine, 61012, Cherenkov, Aleksandr; Kharkiv Petro Vasylenko National Technical University of Agriculture Alchevskih str., 44, Kharkiv, Ukraine, 61012, Pirotti, Evgenij; National Technical University "Kharkiv Polytechnic Institute" Kyrpychova str., 2, Kharkiv, Ukraine, 61002, Moroz, Sergey; Kharkiv Petro Vasylenko National Technical University of Agriculture Alchevskih str., 44, Kharkiv, Ukraine, 61012, Chorna, Mariya; Kharkiv Petro Vasylenko National Technical University of Agriculture Alchevskih str., 44, Kharkiv, Ukraine, 61012, Kosulina, Nataliaya; Kharkiv Petro Vasylenko National Technical University of Agriculture Alchevskih str., 44, Kharkiv, Ukraine, 61012, Cherenkov, Aleksandr; Kharkiv Petro Vasylenko National Technical University of Agriculture Alchevskih str., 44, Kharkiv, Ukraine, 61012, Pirotti, Evgenij; National Technical University "Kharkiv Polytechnic Institute" Kyrpychova str., 2, Kharkiv, Ukraine, 61002, Moroz, Sergey; Kharkiv Petro Vasylenko National Technical University of Agriculture Alchevskih str., 44, Kharkiv, Ukraine, 61012, and Chorna, Mariya; Kharkiv Petro Vasylenko National Technical University of Agriculture Alchevskih str., 44, Kharkiv, Ukraine, 61012

Abstract

The paper is devoted to solution of theoretical and experimental problems in development of electromagnetic (EM) technology in the millimeter wavelength range for disinfection and warming of baled wool during its processing.Sheared wool is an environment well fit for existence of a variety of microorganisms. It was established that 1 kg of wool contains 400 to 7000 million bacteria. This often leads to workers infection with pathogens of brucellosis, myterosis, glanders, toxicoplasmosis, ku-reketmosis, leptospirosis, anthrax and plague.When storing unwashed wool, especially at low temperatures, its compression and fat hardening take place. Fat softens and the rune easily unfolds at temperatures of 25...30 °C. This improves sorting quality and preserves natural wool properties.In this regard, the work studied the regularities of influence of EM energy in the millimeter wavelength range on the pathogenic microorganisms existing in wool.As a result of the studies, a range of changes in the biotropic EMF parameters was determined for destruction of microorganisms. The performed calculations have shown that the biotropic parameters of electromagnetic field include frequency range of 35.3...36.3 GHz; power flux density of 1.25 mW/cm2, exposure time of 2·103 s.Theoretical analysis of the model of warming up baled wool have shown that the temperature rise up to 24...40 °C is possible when wool is exposed to EMF with the following parameters (frequency; power flux density; exposure time): 36 GHz; 1.25...1.5 mW/cm2; 120...180 s.It was established that when wool was exposed to an EMF with certain biotropic parameters, wool breaking load increased by 200...300 cN and relative strength increased by 2...3 cN/tex in comparison with the control runs. The study results make it possible to create an environmentally friendly and efficient technology for primary wool processing by EM energy., Угнетение инфекционных микроорганизмов в кипах шерсти возможно при помощи электромагнитного излучения (ЭМИ) с параметрами: частота 36 ГГц, мощность 0,5 кВт, экспозиция 3 мин. Энергоинформационные параметры были определены в результате теоретических и экспериментальных исследований процесса взаимодействия ЭМИ с инфекционными микроорганизмами, Пригнічення інфекційних мікроорганізмів у кіпах вовни можливо за допомогою електромагнітного випромінювання (ЕМВ) з параметрами: частота 36 ГГц, потужність 0,5 кВт, експозиція 3 хв. Енергоінформаційні параметри були визначені в результаті теоретичних і експериментальних досліджень процесу взаємодії ЕМВ з інфекційними мікроорганізмами

Published
2017

10. Airborne Infectious Microorganisms

Author

Stetzenbach, L.D.

Subjects
SARS, Severe acute respiratory syndrome, bioterrorism, coccidiomycosis, cryptococcosis, WMD, weapons of mass destruction, airborne infectious viruses, severe acute respiratory syndrome, SARS-CoV, SARS-associated coronavirus, bioaerosol, Article, blastomycosis, Nocardia, varicella, airborne infectious bacteria, measles, aspergillosis, hantaviruses, histoplasmosis, NLVs, Norwalk-like viruses, fungi, airborne infectious fungi, smallpox, tuberculosis, Norwalk-like viruses, HPS, Hantavirus pulmonary syndrome, avian influenza, infectious microorganisms, HVAC, Heating, ventilation, and air conditioning, influenza, legionnaires’ disease
Abstract

Inhalation exposes the upper and lower respiratory tracts of humans to a variety of airborne particles and vapors. Airborne transmission of pathogenic microorganisms to humans from the environment, animals, or other humans can result in disease. Inhalation is an important route of exposure as the lung is more susceptible to infection than the gastrointestinal tract. Ingested microorganisms must past through the acidic environment of the stomach before they can colonize tissue while inhaled microorganisms are deposited directly on the moist surfaces of the respiratory tract. Inhalation of microbial aerosols can elicit adverse human health effects including infection, allergic reaction, inflammation, and respiratory disease. Following inhalation, infectious viruses, bacteria, and fungi can establish in host cells of the respiratory tract. Some are translocated and infect the gastrointestinal tract and other tissues. This chapter discusses human viral, bacterial, and fungal diseases transmitted via aerosols. Viral diseases presented are influenza, severe acute respiratory syndrome (SARS), Norwalk-like viruses (NLVs) and hantavirus disease, measles, and varicella. Bacterial diseases are Legionnaires’ disease, tuberculosis, and nontubercule mycobacterial disease. Exposure to some Gram-negative and Gram-positive bacteria, endotoxin, and actinomycetes when dispersed through the air can result in disease following inhalation. Fungal diseases included are histoplasmosis, coccidiomycosis, blastomycosis, cryptococcosis, and aspergillosis. The threat of bioterrorism with airborne infectious agents is also briefly presented.

Published
2009

11. ТЕОРЕТИЧНИЙ АНАЛІЗ ВПЛИВУ НИЗЬКОЕНЕРГЕТИЧНИХ ЕЛЕКТРОМАГНІТНИХ ПОЛІВ ВКРАЙ ВИСОКОЧАСТОТНОГО ДІАПАЗОНУ НА ІНФЕКЦІЙНІ МІКРООРГАНІЗМИ В РАНАХ ШКІРЯНОГО ПОКРИВУ ТВАРИН

Subjects
low-energy electromagnetic field, microwave radiation, the skin of animals, infectious microorganisms, низькоенергетичне електромагнітне поле, випромінювання КВЧ діапазону, шкіряний покрив тварин, інфекційні мікроорганізми
Abstract

Justification of resource-saving electrotechnology with the optimal settings of habitats electromagnetic field and means of radiation of electromagnetic energy to restore the injured skin of farm animals. The article explored the interaction between the electromagnetic fields of ultra-high band of tissue healing and skin of animals by biotropni parameters for EMF suppression of infectious microorganisms in wounds of the skin of its effective recovery. It was proved that biotropnymy parameters for electromagnetic fields suppression of infectious microorganisms and efficient recovery of injured skin animals are: frequency range 35 ... 37 GHz, the power flux density 3 ... 5 mW / cm 2, Exposition 3 ... 5 min. The proposed study on the creation of sources of ultra-high band, which should consist of a transistor (10 GHz) and the diode (over 10 GHz) parts using the method of adding capacity for power within the 2,000-2,500 mWt may be applicable to a broad class of sources high output power. For better recovery of injured skin of animals low-energy electromagnetic fields of millimeter wavelength range, which will create an efficient, energy-saving and environmentally friendly technologies should be used, В даний час для відновлення шкіряного покриву тварин в основному використовують медикаментозні способи лікування. Як показує аналіз літературних джерел, застосування антибіотиків і інших медикаментів не завжди сприяє одужанню тварин і, крім того, лікарські препарати з молоком і м'ясом потрапляють в організм людини, надаючи на нього негативну дію. У статті досліджено процес взаємодії низькенергетичних ЕМП КВЧ діапазону із тканинами ран шкіряного покриву тварин і визначені біотропні параметри ЕМП для пригноблення інфекційних мікроорганізмів у ранах шкіряного покриву з його ефективним відновленням. Доведено, що біотропними параметрами ЕМП для пригноблення інфекційних мікроорганізмів і ефективного відновлення травмованого шкірного покриву тварин є : діапазон частот 35…37 ГГЦ, щільність потоку потужності 3…5 мВт/см2 , експозиція 3…5 хв. Запропоноване обґрунтування по створенню джерел КВЧ діапазону, які повинні складатися із транзисторної ( до 10 ГГЦ ) і діодної ( понад 10 ГГЦ) частин із застосуванням методу додавання потужностей для отримання потужності в межах 2000-2500 мВт, може бути застосовне в для широкого класу джерел з підвищеною вихідною потужністю

Published
2016

12. A Survey on the Level of Containment for Infectious Microorganisms at Hospitals and Biomedical Facilities in Tokyo in Case of Urban Great Disaster

Author

Sugamata, Masami, Yamaori, Junko, Yano, Kazuyoshi, Kimura-kuroda, junko, Ogino, Shuzo, Nakabayashi, Itsuki, and Hoshi, Tanji

Subjects
感染性微生物, Medical Facilities, バイオテロリズム, Great Disaster, 大規模災害, Infectious Microorganisms, Containment, Bioterrorism, 封じ込め, 医学施設
Abstract

東京の特徴の一つとして、病院・病院関連検査機関、および医科学研究系施設が多ぐ存在することが挙げられる。こうした施設では、感染性微生物を含む様々な微生物が検査や研究のために日常的に取り扱われている。これら感染性微生物の取り扱いは、物理学的・生物学的封じ込め(physical and biological containment)の原則に従って行われている。こうした封じ込め対策の機能が、平常時は勿論のこと、地震等の大規模災害等の非常時においては更に厳密に発揮される必要がある。我々は東京都にある上記関係諸機関(, As a characteristic of Tokyo, there are many hospitals that provide treatment to patients with infectious diseases, as well as many biomedical research institutes. Among these facilities, various infectious microorganisms are used routinely for medical di

Published
2007

13. Diversidad microbiana presente en el ambiente de la clínica odontológica de la universidad del Magdalena

Author

Zambrano Gari, César Camilo, Luna Fontalvo, Jorge Alberto, Zambrano Gari, César Camilo, and Luna Fontalvo, Jorge Alberto

Abstract

The microbial diversity in the environment and surface Dental Clinic’s of the University of Magdalena was determined from the search of infectious and pollutant microorganisms such as bacteria and fungi. Four monitorings, taking for each one 16 points of sampling: six trays and seven lamps of different dentals units and theroom’s sterilization, surgery and waiting room were carried out. Sedimentation techniques, static plates and torula, sweep were used for the microbiological environment and surfaces test (trays and lamps of the dentals units). The results shows that the environment of the waiting room had high abundance of bacteria and fungi. The genera Staphylococcus was recovered in higher roportion, with 48.790 CFU/m2/30 minutes and the total count of fungi was 13.150 CFU/m2/30 minutes, in minor scale Pseudomonas, Enterococcus, Moraxella and the total coliforms group. Regarding to the isolated fungi were dentified the genus Aspergillus, Curvularia, Geotrichum and Haplosporangium. The external conditions of the clinic´s environment influence directly on the concentration f microorganisms of the waiting room. The surface´s analysis of the trays and lamps of the dentals units corresponded in high proportion with the microorganisms mentioned previously., Se determinó la diversidad microbiana presente en el ambiente y superficie de la Clínica Odontológica de la Universidad del Magdalena a partir de la búsqueda de microorganismos infecciosos y contaminantes como bacterias y hongos. Se realizaron cuatro monitoreos, en cada uno 16 puntos de muestreo: seis bandejas y siete lámparas de diferentes unidades odontológicas, la sala de esterilización, sala de cirugía y la sala de espera. Se emplearon técnicas de sedimentación en placas estáticas y arrido con tórula para el estudio microbiológico del ambiente y superficies (bandejas y lámparas de las unidades odontológicas) respectivamente. Se encontró que el ambiente de la sala de espera tuvo mayor abundancia de bacterias y hongos. El género Staphylococcus fue recuperado en mayor proporción con 48.790 UFC/m2/30 minutos, y el recuento total de hongos fue de 13.150 UFC/m2/30 minutos. En menor escala se determinaron los géneros bacterianos Pseudomonas, Enterococcus, Moraxella y el grupo de los coliformes totales. Se identificaron hongos de los géneros Aspergillus, Curvularia, Geotrichum y Haplosporangium, demostrando que las condiciones del ambiente externo de a clínica influyen directamente sobre la concentración de microorganismos presentes en la sala de espera. Los resultados obtenidos del análisis de superficies de bandejas y lámparas de las unidades odontológicas correspondieron en mayor proporción con los microorganismos mencionados anteriormente.

Published
2013

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