1. Development of gene delivery systems for the epsilon-poly-L-lysine producer, Streptomyces albulus
- Author
-
Takahiro Kawai, Yusuke Hoshino, Shigeru Nakamori, Hiroshi Takagi, Yoshimitsu Hamano, and Ine Nicchu
- Subjects
biology ,Streptomycetaceae ,Bioengineering ,Gene delivery ,Protoplast ,medicine.disease_cause ,biology.organism_classification ,Applied Microbiology and Biotechnology ,Streptomyces ,Polyethylene Glycols ,Transformation (genetics) ,Drug Delivery Systems ,Shuttle vector ,Biochemistry ,Species Specificity ,Gene Targeting ,medicine ,Escherichia coli ,Polylysine ,Actinomycetales ,Transformation, Bacterial ,Biotechnology - Abstract
Streptomyces albulus IFO14147 produces epsilon-poly-L-lysine (epsilon-PL), an amino acid homopolymer antibiotic that is used as a food preservative in many countries, including Japan, South Korea, and the United States. To construct an overproducer of the industrially important epsilon-PL and to develop a deeper understanding of its biosynthetic mechanism, we developed systems for DNA delivery into the S. albulus strain based on both polyethylene glycol-mediated protoplast transformation and intergeneric conjugation from Escherichia coli. The successes of these transformations in particular were accomplished by employing the new cryptic-plasmid-based shuttle vectors constructed in this study. The genetic systems developed here should facilitate a molecular genetic approach to S. albulus.
- Published
- 2005