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This work showed the crude extract of the endophytic fungus Aspergillus sp, isolated from the almonds of Bertholletia excelsa Humn & Bonlp collected in the Brazilian Amazon, oviposition deterrent, and larvicidal activity of against Aedes aegypti. In the oviposition deterrence test was observed that females able to lay eggs preferred the control oviposition sites (46.6%), suggesting the extract also could repel the oviposition. Futhermore, the extract showed larvicidal activity with LC50 26.86 µg/mL at 24 hours and 18.75 µg/mL at 48 hours. Molecular docking studies were carried out to elucidate the mechanism of action of the compounds identified against the enzyme acetylcholinesterase. The compound Aspergillol B was a potent larvicide with potential for inhibition for the acetylcholinesterase enzyme (-7.74 Kcal/mol). These unprecedented results reported indicate that the secondary metabolites obtained from crude extract of Aspergillus sp. present useful biological potential against vectors of public health importance and antibiotic-resistant bacteria.

Endophytic fungi are microorganisms capable of colonizing the interior of plant tissues without causing damage to them. The study of the secondary metabolites produced by their vast biodiversity fungal is relevant for the discovery of new products for biotechnological and agrochemical applications. In addition, extract of the endophytic fungus Aspergillus sp., isolated from the almonds of Bertholletia excelsa HumnBonlp collected in the Brazilian Amazon, oviposition deterrent, and larvicidal activity of against Aedes aegypti. In the oviposition deterrence test was observed that females able to lay eggs preferred the control oviposition sites (46.6%). Furthermore, the extract showed larvicidal activity with LC50 26.86 µg/mL at 24 h and 18.75 µg/mL at 48 h. Molecular docking studies showed the compound Aspergillol B a potent larvicide by to inhibit the acetylcholinesterase enzyme (- 7.74 kcal/mol). These results indicate that compounds from secondary metabolites of Aspergillus sp., isolated from almonds of B. excelsa, are useful biological potential against vectors A. aegypti.

The amidation of fatty acid is an important reaction for synthesis of fatty alkolamines with a variety of pharmacological activities. The objective of this paper was to demonstrate the aminolysis direct and solvent of the triglycerides from the patawa oil by heterogeneous catalysis. The results of the amidation direct free solvents were favorable using 5% Al2O3 as catalyst, with a conversion of 94% at 24 h of reaction. After spectroscopic characterization, we decided explore the antimicrobial tests of the fatty amides (FAA) against the Staphylococcus aureus strain (ATCC 33591), showing a MIC activity of 1.9612 mg/mL and the fatty ethyl esters (FAEE) a 3.4125 mg/mL MIC. Therefore, the synthesis direct free solvents of FAA by Al2O3 from patawa oil was viable and innovative.

Mosquitoes in the genus Aedes are responsible for the four important human disease viruses, such as, dengue, yellow fever, chikungunya and Zika. Although important advances have emerged in the development of alternative measures for mosquito control, chemical insecticides still remain a vital part of integrated control programs. In this sense, a set of benzylidenemalononitriles were synthetized in very good yields (99-71%), using only water and glycerol at temperature room. A comparative study of the larvicidal activity between benzylidenemalononitriles showed that the 2g (R= 4-Cl) compounds possess much better larvicidal activity (LC50 and LC90 of 9.42 and 15.02, respectively at 24h). Study of molecular docking was applied to identify the mode iteration of compound 2g with binding site at the enzyme acetylcholinesterase, the profile of interaction showed a score 48.9795, with five bonds in three different amino acids.

This paper report the use of Carapa guianensis Abul. (Meliaceae) oil and derivates [fatty acid ethyl ester (FAEE’s) and free fatty acid (FFA’s)] associate with silk fibroin (SF), and its activity against the larvae of the vector Aedes aegypti. The emulsions FFA2-SF derived from AO2, that mostly contained unsaturated fatty acids, presented the best results against the larvae of Ae. aegypti after a 48 h period (LC50 = 16.79 mg.mL−1), causing structural alterations to the vector. It is suggested that the biopolymeric matrix of silk fibroin increases the biodistribution and bioavailability of active in medium aqueous. This is the first work of its type which has pointed larvicidal activity of the free fatty acid from C. guianensis, associate with silk fibroin against Aedes aegypti.

The Aedes aegypti mosquito is a vector that causes various diseases such as chikungunya fever, dengue and Zika, and therefore constitutes a public health problem. The present study was carried out to evaluate the larvicidal activity of different crude extracts (methanolic, hydroethanolic and hexanic) from leaves of Acmella oleracea solubilized in fibroin solution, as an alternative to the organic solvents that are used against Aedes aegypti. Using the Probit analysis, the hexane extract showed LC50 of 2.23 μg/mL after 24 h, whereas the hydroethanolic and the methanolic extracts showed LC50 of 28.42 μg/mL and 39.67 μg/mL, respectively. These extracts also did not show toxicity towards Chlorella vulgaris after 30 days, which suggests that they can be used without causing environmental damage. Thus, A. oleracea extract dissolved in fibroin solution could be an alternative for controlling Ae. aegypti without causing damage to the environment.