1. Rapid and robust analytical protocol for E. coli STEC bacteria subspecies differentiation using whole cell MALDI mass spectrometry.
- Author
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Mclean K, Palarea-Albaladejo J, Currie CG, Imrie LHJ, Manson EDT, Fraser-Pitt D, Wright F, Alexander CJ, Pollock KGJ, Allison L, Hanson M, and Smith DGE
- Subjects
- Bacterial Typing Techniques methods, Principal Component Analysis, Serogroup, Species Specificity, Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization statistics & numerical data, Time Factors, Bacterial Typing Techniques statistics & numerical data, Escherichia coli O157 isolation & purification, Shiga-Toxigenic Escherichia coli isolation & purification, Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization methods
- Abstract
Whole cell MALDI is regularly used for the identification of bacteria to species level in clinical Microbiology laboratories. However, there remains a need to rapidly characterize and differentiate isolates below the species level to support outbreak management. We describe the implementation of a modified preparative approach for MALDI-MS combined with a custom analytical computational pipeline as a rapid procedure for subtyping Shigatoxigenic E. coli (STEC) and accurately identifying strain-specifying biomarkers. The technique was able to differentiate E. coli O157:H7 from other STEC. Within O157 serotype O157:H7 isolates were readily distinguishable from Sorbitol Fermenting O157 isolates. Overall, nine homogeneous groups of isolates were distinguished, each exhibiting distinct profiles of defining mass spectra features. This offers a robust analytical tool useable in reference/diagnostic public health scenarios., (Crown Copyright © 2018. Published by Elsevier B.V. All rights reserved.)
- Published
- 2018
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