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5. Application of cinnamoyl derivative as a new ligand for dispersive liquid-liquid microextraction and spectrophotometric determination of cobalt

Author

Eleckova, L., Balogh, I.S., Imrich, J., and Andruch, V.

Subjects
Toluene -- Laws, regulations and rules -- Chemical properties -- Research, Extraction (Chemistry) -- Methods -- Research, Cobalt -- Chemical properties -- Research, Chemistry
Abstract

The method is based on the reaction of Co(II) with 3-[4-(dimethylamino)cinnamoyl]-4-hydroxy-6-methyl-2H-pyran-2-one (ligand) and a dimethylindocarbocyanine dye, followed by dispersive liquid-liquid microextraction of the ion associate formed and subsequent spectrophotometric detection. The appropriate experimental conditions were found to be: pH 9, 0.14 mM dye, toluene (extraction solvent) containing 2.25 mM of ligand, acetonitrile (dispersive solvent) and carbon tetrachloride (auxiliary solvent). Beer's law is obeyed in the range 0.06-0.42 mg/L of Co(II) at a wavelength of 558 nm. The limit of detection, calculated as three times the standard deviation of the blank test (n = 10), was found to be 9 µg/L for Co(II). The method was applied to the determination of cobalt in spiked water samples and a vitamin B12 pharmaceutical injection. Keywords: cinnamoyl pyrones, cobalt determination, dispersive liquid-liquid microextraction, UV-Vis detection, vitamin B12 DOI: 10.1134/S1061934815030053, Cobalt is an essential trace element for humans, but at high levels it can be toxic to human health. Cobalt toxicology is discussed in the publication by Domingo [1]. Cobalt [...]

Published
2015
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38. Multiplex sequencing of 1.5 Mb of the Mycobacterium leprae genome.

Author

Smith, D R, Richterich, P, Rubenfield, M, Rice, P W, Butler, C, Lee, H M, Kirst, S, Gundersen, K, Abendschan, K, Xu, Q, Chung, M, Deloughery, C, Aldredge, T, Maher, J, Lundstrom, R, Tulig, C, Falls, K, Imrich, J, Torrey, D, Engelstein, M, Breton, G, Madan, D, Nietupski, R, Seitz, B, Connelly, S, McDougall, S, Safer, H, Gibson, R, Doucette-Stamm, L, Eiglmeier, K, Bergh, S, Cole, S T, Robison, K, Richterich, L, Johnson, J, Church, G M, and Mao, J I

Abstract

The nucleotide sequence of 1.5 Mb of genomic DNA from Mycobacterium leprae was determined using computer-assisted multiplex sequencing technology. This brings the 2.8-Mb M. leprae genome sequence to approximately 66% completion. The sequences, derived from 43 recombinant cosmids, contain 1046 putative protein-coding genes, 44 repetitive regions, 3 tRNAs, and 15 tRNAs. The gene density of one per 1.4 kb is slightly lower than that of Mycoplasma (1.2 kb). Of the protein coding genes, 44% have significant matches to genes with well-defined functions. Comparison of 1157 M. leprae and 1564 Mycobacterium tuberculosis proteins shows a complex mosaic of homologous genomic blocks with up to 22 adjacent proteins in conserved map order. Matches to known enzymatic, antigenic, membrane, cell wall, cell division, multidrug resistance, and virulence proteins suggest therapeutic and vaccine targets. Unusual features of the M. leprae genome include large polyketide synthase (pks) operons, inteins, and highly fragmented pseudogenes.

Published
1997

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