Search

Crash databases are one of the primary data sources for road safety research. Therefore, their quality is fundamental for the accuracy of crash analyses and, consequently the design of effective countermeasures. Although crash data often suffer from correctness and completeness issues, these are rarely discussed or addressed in crash analyses. Crash reports aim to answer the five "W" questions (i.e. When?, Where?, What?, Who? and Why?) of each crash by including a range of attributes. This paper reviews current literature on the state of crash data quality for each of these questions separately. The most serious data quality issues appear to be: inaccuracies in crash location and time, difficulties in data linkage (e.g. with traffic data) due to inconsistencies in databases, severity misclassification, inaccuracies and incompleteness of involved users' demographics and inaccurate identification of crash contributory factors. It is shown that the extent and the severity of data quality issues are not equal between attributes and the level of impact in road safety analyses is not yet entirely known. This paper highlights areas that require further research and provides some suggestions for the development of intelligent crash reporting systems., (Copyright © 2017 Elsevier Ltd. All rights reserved.)

Recent technological advancements bring the Connected and Autonomous Vehicles (CAVs) era closer to reality. CAVs have the potential to vastly improve road safety by taking the human driver out of the driving task. However, the evaluation of their safety impacts has been a major challenge due to the lack of real-world CAV exposure data. Studies that attempt to simulate CAVs by using either a single or integrating multiple simulation platforms have limitations, and in most cases, consider a small element of a network (e.g. a junction) and do not perform safety evaluations due to inherent complexity. This paper addresses this problem by developing a decision-making CAV control algorithm in the simulation software VISSIM, using its External Driver Model Application Programming Interface. More specifically, the developed CAV control algorithm allows a CAV, for the first time, to have longitudinal control, search adjacent vehicles, identify nearby CAVs and make lateral decisions based on a ruleset associated with motorway traffic operations. A motorway corridor within M1 in England is designed in VISSIM and employed to implement the CAV control algorithm. Five simulation models are created, one for each weekday. The baseline models (i.e. CAV market penetration: 0%) are calibrated and validated using real-world minute-level inductive loop detector data and also data collected from a radar-equipped vehicle. The safety evaluation of the proposed algorithm is conducted using the Surrogate Safety Assessment Model (SSAM). The results show that CAVs bring about compelling benefit to road safety as traffic conflicts significantly reduce even at relatively low market penetration rates. Specifically, estimated traffic conflicts were reduced by 12-47%, 50-80%, 82-92% and 90-94% for 25%, 50%, 75% and 100% CAV penetration rates respectively. Finally, the results indicate that the presence of CAVs ensured efficient traffic flow., (Copyright © 2019 Elsevier Ltd. All rights reserved.)

Among other cells, macrophages regulate the inflammatory and reparative phases during wound healing but genetic determinants and detailed molecular pathways that modulate these processes are not fully elucidated. Here, we took advantage of normal variation in wound healing in 1,378 genetically outbred mice, and carried out macrophage RNA-sequencing profiling of mice with extreme wound healing phenotypes (i.e., slow and fast healers, n = 146 in total). The resulting macrophage coexpression networks were genetically mapped and led to the identification of a unique module under strong trans-acting genetic control by the Runx2 locus. This macrophage-mediated healing network was specifically enriched for cholesterol and fatty acid biosynthetic processes. Pharmacological blockage of fatty acid synthesis with cerulenin resulted in delayed wound healing in vivo, and increased macrophage infiltration in the wounded skin, suggesting the persistence of an unresolved inflammation. We show how naturally occurring sequence variation controls transcriptional networks in macrophages, which in turn regulate specific metabolic pathways that could be targeted in wound healing.

Speed variations are identified as potentially important predictors of freeway crash rates; however, their impacts on crashes are not entirely known. Existing findings tend to be inconsistent possibly because of the different definitions for speed variations, different crash type consideration or different modelling and data aggregation approaches. This study explores the relationships of speed variations with crashes on a freeway section in the UK. Crashes split by vehicle type (heavy and light vehicles) and by severity mode (killed/serious injury and slight injury crashes) are aggregated based on the similarities of the conditions just before their occurrence (condition-based approach) and modelled using Multivariate Poisson lognormal regression. The models control for speed variations along with other traffic and weather variables as well as their interactions. Speed variations are expressed as two separate variables namely the standard deviations of speed within the same lane and between-lanes over a five-minute interval. The results, similar for all crash types (by coefficient significance and sign), suggest that crash rates increase as the within lane speed variations raise, especially at higher traffic volumes. Higher speeds coupled with greater volume and high between-lanes speed variation also increase crash likelihood. Overall, the results suggest that specific combinations of traffic characteristics increase the likelihood of crash occurrences rather than their individual effects. Identification of these specific crash prone conditions could improve our understanding of crash risk and would support the development of more efficient safety countermeasures., (Copyright © 2018 Elsevier Ltd. All rights reserved.)

Secondary crashes (SCs) or crashes that occur within the boundaries of the impact area of prior, primary crashes are one of the incident types that frequently affect highway traffic operations and safety. Existing studies have made great efforts to explore the underlying mechanisms of SCs and relevant methodologies have been evolving over the last two decades concerning the identification, modeling, and prevention of these crashes. So far there is a lack of a detailed examination on the progress, lessons, and potential opportunities regarding existing achievements in SC-related studies. This paper provides a comprehensive investigation of the state-of-the-art approaches; examines their strengths and weaknesses; and provides guidance in exploiting new directions in SC-related research. It aims to support researchers and practitioners in understanding well-established approaches so as to further explore the frontiers. Published studies focused on SCs since 1997 have been identified, reviewed, and summarized. Key issues concentrated on the following aspects are discussed: (i) static/dynamic approaches to identify SCs; (ii) parametric/non-parametric models to analyze SC risk, and (iii) deployable countermeasures to prevent SCs. Based on the examined issues, needs, and challenges, this paper further provides insights into potential opportunities such as: (a) fusing data from multiple sources for SC identification, (b) using advanced learning algorithms for real-time SC analysis, and (c) deploying connected vehicles for SC prevention in future research. This paper contributes to the research community by providing a one-stop reference for research on secondary crashes., (Copyright © 2018 Elsevier Ltd. All rights reserved.)

To understand the population genetics of structural variants and their effects on phenotypes, we developed an approach to mapping structural variants that segregate in a population sequenced at low coverage. We avoid calling structural variants directly. Instead, the evidence for a potential structural variant at a locus is indicated by variation in the counts of short-reads that map anomalously to that locus. These structural variant traits are treated as quantitative traits and mapped genetically, analogously to a gene expression study. Association between a structural variant trait at one locus, and genotypes at a distant locus indicate the origin and target of a transposition. Using ultra-low-coverage (0.3×) population sequence data from 488 recombinant inbred Arabidopsis thaliana genomes, we identified 6502 segregating structural variants. Remarkably, 25% of these were transpositions. While many structural variants cannot be delineated precisely, we validated 83% of 44 predicted transposition breakpoints by polymerase chain reaction. We show that specific structural variants may be causative for quantitative trait loci for germination and resistance to infection by the fungus Albugo laibachii , isolate Nc14. Further we show that the phenotypic heritability attributable to read-mapping anomalies differs from, and, in the case of time to germination and bolting, exceeds that due to standard genetic variation. Genes within structural variants are also more likely to be silenced or dysregulated. This approach complements the prevalent strategy of structural variant discovery in fewer individuals sequenced at high coverage. It is generally applicable to large populations sequenced at low-coverage, and is particularly suited to mapping transpositions., (Copyright © 2017 by the Genetics Society of America.)

The aim of expression Quantitative Trait Locus (eQTL) mapping is the identification of DNA sequence variants that explain variation in gene expression. Given the recent yield of trait-associated genetic variants identified by large-scale genome-wide association analyses (GWAS), eQTL mapping has become a useful tool to understand the functional context where these variants operate and eventually narrow down functional gene targets for disease. Despite its extensive application to complex (polygenic) traits and disease, the majority of eQTL studies still rely on univariate data modeling strategies, i.e., testing for association of all transcript-marker pairs. However these "one at-a-time" strategies are (1) unable to control the number of false-positives when an intricate Linkage Disequilibrium structure is present and (2) are often underpowered to detect the full spectrum of trans-acting regulatory effects. Here we present our viewpoint on the most recent advances on eQTL mapping approaches, with a focus on Bayesian methodology. We review the advantages of the Bayesian approach over frequentist methods and provide an empirical example of polygenic eQTL mapping to illustrate the different properties of frequentist and Bayesian methods. Finally, we discuss how multivariate eQTL mapping approaches have distinctive features with respect to detection of polygenic effects, accuracy, and interpretability of the results.

Finding causal relationships between genotypic and phenotypic variation is a key focus of evolutionary biology, human genetics and plant breeding. To identify genome-wide patterns underlying trait diversity, we assembled a high-quality reference genome of Cardamine hirsuta, a close relative of the model plant Arabidopsis thaliana. We combined comparative genome and transcriptome analyses with the experimental tools available in C. hirsuta to investigate gene function and phenotypic diversification. Our findings highlight the prevalent role of transcription factors and tandem gene duplications in morphological evolution. We identified a specific role for the transcriptional regulators PLETHORA5/7 in shaping leaf diversity and link tandem gene duplication with differential gene expression in the explosive seed pod of C. hirsuta. Our work highlights the value of comparative approaches in genetically tractable species to understand the genetic basis for evolutionary change.