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Your search keyword '"Immobilized proteases"' showing total 2 results
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2 results on '"Immobilized proteases"'

1. Support Enzyme Loading Influences the Effect of Aldehyde Dextran Modification on the Specificity of Immobilized Ficin for Large Proteins.

Author

Siar, El Hocine, Abellanas-Perez, Pedro, Rocha-Martin, Javier, and Fernandez-Lafuente, Roberto

Subjects
*ENZYME specificity, *ENZYMES, *STERIC hindrance, *ALDEHYDES, *DEXTRAN
Abstract

It has been reported that the modification of immobilized glyoxyl–ficin with aldehyde dextran can promote steric hindrances that greatly reduce the activity of the immobilized protease against hemoglobin, while the protease still maintained a reasonable level of activity against casein. In this paper, we studied if this effect may be different depending on the amount of ficin loaded on the support. For this purpose, both the moderately loaded and the overloaded glyoxyl–ficin biocatalysts were prepared and modified with aldehyde dextran. While the moderately loaded biocatalyst had a significantly reduced activity, mainly against hemoglobin, the activity of the overloaded biocatalyst was almost maintained. This suggests that aldehyde dextran was able to modify areas of the moderately loaded enzyme that were not available when the enzyme was overloaded. This modification promoted a significant increase in biocatalyst stability for both biocatalysts, but the stability was higher for the overloaded biocatalyst (perhaps due to a combination of inter- and intramolecular crosslinking). [ABSTRACT FROM AUTHOR]

Published
2024
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2. Applications of spaceborne radar laboratory data to the study of aeolian processes

Author

Anthony R. Dobrovolskis, Bruce R. White, Dan G. Blumberg, Stephen D. Wall, Ronald Greeley, Keld Rømer Rasmussen, James D. Iversen, J. F. McHone, and Nicholas Lancaster

Subjects
B/E linked scan, Atmospheric Science, L band, F-compounds, C band, Immobilized proteases, Soil Science, Surface finish, Aquatic Science, Oceanography, MALDI-MS, Wind speed, law.invention, Peptide mapping, Geochemistry and Petrology, law, Earth and Planetary Sciences (miscellaneous), Surface roughness, Radar, Earth-Surface Processes, Water Science and Technology, Remote sensing, Tryptic digestion, Ecology, Paleontology, Si-compounds, Forestry, Metastable ion, Geophysics, Space and Planetary Science, Aeolian processes, Protein identification, Scale (map), Ortho-effect, Geology
Abstract

Aerodynamic roughness (z0) is an important parameter in studies of sand and dust transport, as well as atmospheric circulation models. Aerodynamic roughness is a function of the size and spacing of surface roughness elements and is typically determined at point locations in the field from wind velocity profiles. Because field measurements require complex logistics, z0 values have been obtained for very few localities. If radar can be used to map z0, estimates can be obtained for large areas. In addition, because aerodynamic roughness can change in response to surface processes (e.g., flooding of alluvial surfaces), radar remote sensing could obtain new measurements on short timescales. Both z0 and the radar backscatter coefficient σ0 are dependent on topographic roughness at the submeter scale, and correlation between, these two parameters was developed based on radar data obtained from aircraft (AIRSAR). The Spaceborne Radar Laboratory (SRL) afforded the opportunity to test the correlation for data obtained from orbit. SRL data for sites in Death Valley, California; Lunar Lake, Nevada; and Gobabeb, Namibia, were correlated with wind data and compared with previous radar z0 relations. Correlations between σ0 and z0 for L band (λ = 24 cm) HV (H, vertically and V, vertically polarized modes) L band HH, and C band (λ = 5.6 cm) HV compare favorably with previous studies. Based on these results, maps of z0 values were derived from SRL data for each site, demonstrating the potential to map z0 for large vegetation-free areas from orbit using radar systems.

Published
1997
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