Search

Biomaterials of natural origin have recently gained increasing attention in the field of dental implantology. The requirements for such materials, however, are very high. In addition to high clinical efficiency in tissue regeneration, wound healing should be demonstrably positively influenced. The translational division for regenerative orofacial medicine of the Clinic and Polyclinic for Oral and Maxillofacial Surgery of the University Medical Center Hamburg-Eppendorf (UKE) is examining this research topic by investigating which innovative treatment methods for the reconstruction of bone defects or for augmentative procedures can be applied in the future or are already being applied in the field of oral and maxillofacial surgery.Biomaterialien natürlichen Ursprungs im Bereich der dentalen Implantologie erhalten in den letzten Jahren vermehrt Aufmerksamkeit, jedoch sind die Anforderungen hoch. Neben einer hohen klinischen Effizienz bei der Geweberegeneration sollte die Wundheilung nachweislich positiv beeinflusst werden. Die translationale Sektion „Regenerative Orofaziale Medizin“ der Klinik und Poliklinik für Mund-, Kiefer- und Gesichtschirurgie der Uniklinik Hamburg-Eppendorf (UKE) beschäftigt sich mit diesem Thema und untersucht, welche innovativen Behandlungsmethoden zur Rekonstruktion von Knochendefekten oder bei augmentativen Verfahren im Bereich der Mund-, Kiefer- und Gesichtschirurgie zukünftig Anwendung finden oder bereits angewendet werden.

Oral wounds are among the most troublesome injuries which easily affect the patients' quality of life. To date, the development of functional antibacterial dressings for oral wound healing remains a challenge. In this regard, we investigated antibacterial silk protein-based membranes for the application as wound dressings in oral and maxillofacial surgery. The present study includes five variants of casted membranes, i.e., i) membranes-silver nanoparticles (CM-Ag), ii) membranes-gentamicin (CM-G), iii) membranes-control (without functionalization) (CM-C), iv) membranes-silk sericin control (CM-SSC), and v) membranes-silk fibroin/silk sericin (CM-SF/SS), and three variants of nonwovens, i.e., i) silver nanoparticles (NW-Ag), ii) gentamicin (NW-G), iii) control (without functionalization) (NW-C). The surface structure of the samples was visualized with scanning electron microscopy. In addition, antibacterial testing was accomplished using agar diffusion assay, colony forming unit (CFU) analysis, and qrt-PCR. Following antibacterial assays, biocompatibility was evaluated by cell proliferation assay (XTT), cytotoxicity assay (LDH), and live-dead assay on L929 mouse fibroblasts. Findings indicated significantly lower bacterial colony growth and DNA counts for CM-Ag with a reduction of bacterial counts by 3log levels (99.9% reduction) in CFU and qrt-PCR assay compared to untreated control membranes (CM-C and CM-SSC) and membranes functionalized with gentamicin (CM-G and NW-G) (p 0.001). Similarly, NW-G yielded significantly lower DNA and colony growth counts compared to NW-Ag and NW-C (p 0.001). In conclusion, CM-Ag represented 1log level better antibacterial activity compared to NW-G, whereas NW-G showed better cytocompatibility for L929 cells. As data suggest, these two membranes have the potential of application in the field of bacteria-free oral wound healing. However, provided that loading strategy and cytocompatibility are adjusted according to the antibacterial agents' characteristic and fabrication technique of the membranes.

Die tanztherapeutische Methode der Authentischen Bewegung ermoglicht eine kreative, korper- und bewegungsorientierte Suche nach dem Selbst. In diesem Artikel wird der geschutzte tanztherapeutische Rahmen beschrieben, der gegeben sein muss, damit sich festgefahrene psychophysische Muster wandeln konnen und transformiert werden.