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135 results on '"Imbenotte M"'

4. Determination of glyphosate in biological fluids by (super 1)H and (super 31)P NMR spectroscopy.

Author

Cartigny, B., Azaroual, N., Imbenotte, M., Mathieu, D., Vermeersch, G., Goulle, J.P., and Lhermitte, M.

Subjects
Poisoning -- Research, Glyphosate -- Analysis -- Research -- Usage, Nuclear magnetic resonance -- Usage -- Research -- Analysis, Xenobiotics -- Research, Body fluids -- Research -- Analysis -- Usage
Abstract

Abstract Identification of glyphosate in four cases of poisoning, using nuclear magnetic resonance spectroscopy of biological fluids is reported. It has been performed by using a combination of ¹H and [...]

Published
2004

10. Study of ethnic differences in distribution of CYP3A5 gene polymorphisms

Author

S Chevalier D Allorge D Lo-Guidice Jm Migot-Nabias F Kenani a Imbenotte M Broly F Lacarelle B Lhermitte M., Quaranta, Cytosquelette et Intégration des Signaux du Micro-Environnement Tumoral - UMR 6032 (CISMET), Université de la Méditerranée - Aix-Marseille 2-Université de Provence - Aix-Marseille 1-Centre National de la Recherche Scientifique (CNRS), and Vidalin, Michèle

Subjects
[SDV.BC]Life Sciences [q-bio]/Cellular Biology, [SDV.BC] Life Sciences [q-bio]/Cellular Biology, ComputingMilieux_MISCELLANEOUS
Abstract

International audience

Published
2006

28. Ethnic differences in the distribution of CYP3A5 gene polymorphisms.

Author

Quaranta, S., Chevalier, D., Allorge, D., Lo-Guidice, J. M., Migot-Nabias, F., Kenani, A., Imbenotte, M., Broly, F., Lacarelle, B., and Lhermitte, M.

Subjects
ETHNIC groups, GENETIC polymorphism research, CYTOCHROMES, ALLELES, GENOTYPE-environment interaction, POLYMERASE chain reaction, TUNISIANS, CAUCASIAN race
Abstract

The genetic polymorphism affecting the CYP3A5 enzyme is responsible for interindividual and interethnic variability in the metabolism of CYP3A5 substrates. The full extent of the CYP3A5 genetic polymorphism was analysed in French Caucasian, Gabonese and Tunisian populations using a polymerase chain reaction-single strand conformational polymorphism (PCR-SSCP) strategy. In the three populations, eight, 17 and ten single nucleotide polymorphisms (SNPs), respectively, were identified, among which nine correspond to rare new mutations. Also identified were 16 alleles including eight new allelic variants. Significant differences were observed in the distribution of these alleles. Particularly, the frequency of the CYP3A5*3C null allele in French Caucasians (81.3%) and in Tunisians (80.0%) is higher than in the Gabonese population (12.5%) (p < 0.001). Considering the CYP3A5 genotypes of the tested individuals, only 10.4% of French Caucasians and 30.0% of Tunisians were identified as CYP3A5 expressors. In contrast, 90.0% of Gabonese subjects appear to express the CYP3A5 protein. [ABSTRACT FROM AUTHOR]

Published
2006
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30. Identification and quantitation of xenobiotics by 1H NMR spectroscopy in poisoning cases.

Author

Imbenotte, M., Azaroual, N., Cartigny, B., Vermeersch, G., and Lhermitte, M.

Subjects
*XENOBIOTICS, *NUCLEAR magnetic resonance
Abstract

In order to analyse a wide range of xenobiotics and their metabolites present in biological fluids, NMR spectroscopy can be used. A large variety of xenobiotics (therapeutic agents, pesticides, solvents, alcohols) can be characterized and quantitated directly, without sample preparation. NMR investigations were applied to acute poisoning cases, involving drugs such as salicylates and valproic acid (VPA). In a salicylate poisoning case, the three major metabolites of acetylsalicylic acid have been detected in crude urine, and rapid identification of lysine revealed the origin of the intoxication, namely lysine acetylsalicylate (Aspegic®). Valproic acid as its glucuronide was identified in urine samples from two poisoned patients. 1H NMR was also used to identify and quantitate paraquat (Gramoxone®) in urine owing to its two aromatic signals at 8.49 and 9.02 ppm, in two acutely poisoned patients (183 and 93 mg/l). An intentional poisoning case with tetrahydrofuran (THF) was also investigated. Serum and urine samples were collected. THF was characterized by its resonances at 1.90 and 3.76 ppm, and quantified at 813 and 850 mg/l in the two biological fluids, respectively. Moreover, two other compounds were detected: lactate and γ-hydroxybutyric acid (GHB). 1H NMR spectroscopic analysis of serum samples from three poisoned patients revealed methanol in one case and ethylene glycol in the two others. Moreover, in the same spectrum, the corresponding metabolites formate and glycolate were found. Compared with the reference chromatographic or spectrophotometric methods, requiring time-consuming extraction and/or derivatization steps, NMR spectroscopy allows the determination of many exogenous and endogenous compounds, without any pre-selection of the analytes. [Copyright &y& Elsevier]

Published
2003
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31. Valproic acid intoxication identified by1H and1H-13C correlated NMR spectroscopy of urine samples.

Author

Azaroual, N., Imbenotte, M., Cartigny, B., Leclerc, F., Vallée, L., Lhermitte, M., and Vermeersch, G.

Abstract

Analysis of biological fluids by proton and carbon nuclear magnetic resonance spectroscopy (1H and13C NMR) is a promising tool in clinical biology. We used this method for rapid toxicological screening in the case of two suicide attempts. For each case, a urine sample was analysed at 300 MHz by 1D and 2D sequences (TOCSY and HMBC) in a short experimental time. Quantification was performed by peak integration on the 1D1H NMR spectrum. For the two patients, results showed the same resonances of the major metabolite, valproyl- O-glucuronide at concentrations of 121 and 44 mmol/l. [ABSTRACT FROM AUTHOR]

Published
2000
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36. Simultaneous determination of tryptophan and kynurenine in serum by HPLC with UV and fluorescence detection.

Author

Vignau, J., Jacquemont, M.-C., Lefort, A., Imbenotte, M., and Lhermitte, M.

Abstract

Tryptophan metabolism is disturbed in mental depression, and the induction of indoleamine 2,3-dioxygenase increases kynurenine production. In order to determine this disturbance in patients with chronic hepatitis C and receiving interferon-based immunotherapy, a new and specific HPLC protocol was elaborated. For tryptophan, the assay was linear from 6.25 to 100 µmol L−1, and the limits of detection (LOD) and quantitation (LOQ) for the method were 0.7 and 8.0 µmol L−1. For kynurenine, the linearity of calibration was from 0.0625 to 6.25 µmol L−1, with LOD and LOQ of 2 and 3 nmol L−1. Reproducibility and repeatability were satisfactory. The method allowed study of human blood serum. Copyright © 2004 John Wiley & Sons, Ltd. [ABSTRACT FROM AUTHOR]

Published
2004
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38. <SUP>1</SUP>H NMR Investigation of Toxic Effects of Lindane and Paraquat on Hep 3B and Hep G2 Human Hepatoma Cell Lines

Author

Descampiaux, B., Imbenotte, M., Desenclos, V., Vermeersch, G., Lhermitte, M., and Erb, F.

Abstract

Lindane and paraquat induce biochemical changes in the liver. In order to specify their molecular impact at the cellular level, a 300 MHz 1H NMR investigation of hepatoma cell lines Hep 3B and Hep G2 responses was performed. Cells were exposed over 24 h to 50 mg/L lindane (0.178 mM) or to 100 mg/L (0.389 mM) paraquat concentrations. The main observation following exposure to lindane was a decrease in betaine methyl groups (3.26 ppm) which could be related to the steatosis reported by some authors. Specifically, in Hep G2 cells with this pesticide, the glycine peak (3.56 ppm) was lowered, thus confirming that the glycine synthesis pathway involving methionine, choline, and betaine was disturbed by lindane. Moreover, in this hepatoma cell line, the p-chlorobenzoate ion could be detected as a doublet at 7.55 ppm. In Hep 3B cells, paraquat increased betaine and methionine levels, suggesting disturbance in glycine biosynthesis. Possibilities of cellular uptake were considered, and the presence of this herbicide in cells was revealed by spectrophotometric and NMR measurements after chlorhydric hydrolysis, suggesting interaction with cellular components. The impact of paraquat on Hep G2 cells appeared to be located on mitochondrial function, as indicated by the observed decrease in succinate and pyruvate levels.

Published
1997

39. Incidence de la complexation sur la toxicite du cadmium et du cuivre vis a vis de daphnia magna

Author

Pommery, J., Imbenotte, M., and Erb, F.

Abstract

Differential pulse anodic stripping voltametry and equilibrium computation were used to determine the levels of copper and cadmium, in free ionic state and in complexed species, under the effect of EDTA and HK2PO4. A variation of short term toxicity between the two chemical species was observed on the daphnia.The results of this study lead to conclusions on the stoechiometry and stability of these complexes, and on the determination of the toxic chemical species.

Published
1984
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44. Use of acetaminophen in young subelite athletes

Author

Garcin, M., Laurence Hamard, Billat, V., Imbenotte, M., Humbert, L., and Lhermitte, M.

Subjects
Adult, Male, Pain Threshold, Adolescent, Pain, Self Medication, Analgesics, Non-Narcotic, Urinalysis, Bicycling, Running, Case-Control Studies, Acute Disease, Chronic Disease, Humans, Female, Acetaminophen, Sports
Abstract

The purpose of the present investigation was to look for other drugs besides doping substances in the urine of subelite athletes submitted to heavy training.One hundred and forty-one young subelite athletes (in sprint, cycling, middle distance running and handball) were included in the study, with a control group of 89 high school pupils. Drugs were researched by high performance liquid chromatography using a diode array detector.Among the 212 subjects who agreed to give a urine sample, acetaminophen was detected: 9.5% for the subelite athletes versus 1.3% for the control group with a greater difference for sprint and cycling training (26.7% and 20%, respectively). Acetaminophen is used to treat both acute and chronic pains. It relieves pain by elevating the pain threshold.The use of acetaminophen has to be taken into account by medical staff, trainers and educators.

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