Search

Your search keyword '"Ilicic, Katarina"' showing total 20 results
Start Over Author "Ilicic, Katarina"
20 results on '"Ilicic, Katarina"'

2. Neutron-activated, plasmonically excitable Fe-Pt-Yb2O3 nanoparticles delivering anti-cancer radiation against human glioblastoma brain cancer cells

Author

Seemann, Klaus M., primary, Kovács, András, additional, Schmid, Thomas E., additional, Ilicic, Katarina, additional, Multhoff, Gabriele, additional, Dunin-Borkowski, Rafal E., additional, Michelagnoli, Caterina, additional, Cieplicka-Oryńczak, Natalia, additional, Jana, Soumen, additional, Colombi, Giacomo, additional, Jentschel, Michael, additional, Schneider, Claus M., additional, and Kuhn, Bernd, additional

Published
2023
Full Text
View/download PDF

7. Proton pencil minibeam irradiation of an in-vivo mouse ear model spares healthy tissue dependent on beam size

Author

Sammer, Matthias, primary, Zahnbrecher, Esther, additional, Dobiasch, Sophie, additional, Girst, Stefanie, additional, Greubel, Christoph, additional, Ilicic, Katarina, additional, Reindl, Judith, additional, Schwarz, Benjamin, additional, Siebenwirth, Christian, additional, Walsh, Dietrich W. M., additional, Combs, Stephanie E., additional, Dollinger, Günther, additional, and Schmid, Thomas E., additional

Published
2019
Full Text
View/download PDF

8. Die zelluläre Antwort auf Bestrahlung mit fokussierten Niedrig-LET Protonen im Vergleich zu Hoch-LET Bestrahlung

Author

Ilicic, Katarina, Multhoff, Gabriele (Prof. Dr.), and Schnieke, Angelika (Prof., Ph.D.)

Subjects
Biowissenschaften, Biologie, ddc:570, RBE, LET, cellular response, microbeam irradiation
Abstract

The clinical use of high linear energy transfer (LET) particles in radiotherapy offers many benefits in comparison to conventional photon radiotherapy particularly due to an inhomogeneous dose deposition and thus an enhanced relative biological effectiveness (RBE). This work gives new insight into the dependence of the RBE on spatial dose distribution using a microbeam of low LET protons which were applied to cell nuclei in several variations and the resulting cellular response was assessed. The focused dose application resulted in an increased RBE, thus simulating high LET effects. Der Einsatz von Hoch-LET Teilchen in der Strahlentherapie bietet viele Vorteile gegenüber der konventionellen Photonenbestrahlung, da ihre inhomogene Dosisverteilung eine erhöhte relative biologische Wirksamkeit (RBW) bedingt. Diese Arbeit gibt neue Einblicke in die Abhängigkeit der RBW von der räumlichen Dosisverteilung. Hierfür wurden Niedrig-LET Protonen mit Hilfe eines Mikrostrahls in Zellkerne appliziert und die zelluläre Antwort untersucht. Die fokussierte Dosisapplikation führte zu einer erhöhten RBW, welche mit Hoch-LET Effekten vergleichbar ist.

Published
2018

9. DNA damage interactions on both nanometer and micrometer scale determine overall cellular damage

Author

Ilicic, Katarina, Greubel, Christoph, Girst, Stefanie, Reindl, Judith, Sammer, Matthias, Schwarz, Benjamin, Siebenwirth, Christian, Walsh, Dietrich W. M., Schmid, Thomas E., Scholz, Michael, and Dollinger, Günther

Abstract

Scientific reports 8(1), 16063 (2018). doi:10.1038/s41598-018-34323-9, Published by Macmillan Publishers Limited, part of Springer Nature, [London]

Published
2018
Full Text
View/download PDF

10. Beam size limit for pencil minibeam radiotherapy determined from side effects in an in-vivo mouse ear model

Author

Sammer, Matthias, primary, Teiluf, Katharina, additional, Girst, Stefanie, additional, Greubel, Christoph, additional, Reindl, Judith, additional, Ilicic, Katarina, additional, Walsh, Dietrich W. M., additional, Aichler, Michaela, additional, Walch, Axel, additional, Combs, Stephanie E., additional, Wilkens, Jan J., additional, Dollinger, Günther, additional, and Schmid, Thomas E., additional

Published
2019
Full Text
View/download PDF

11. The cellular responses to focused low LET proton irradiation in comparison to high LET irradiation

Author

Multhoff, Gabriele (Prof. Dr.), Schnieke, Angelika (Prof., Ph.D.), Ilicic, Katarina, Multhoff, Gabriele (Prof. Dr.), Schnieke, Angelika (Prof., Ph.D.), and Ilicic, Katarina

Abstract

The clinical use of high linear energy transfer (LET) particles in radiotherapy offers many benefits in comparison to conventional photon radiotherapy particularly due to an inhomogeneous dose deposition and thus an enhanced relative biological effectiveness (RBE). This work gives new insight into the dependence of the RBE on spatial dose distribution using a microbeam of low LET protons which were applied to cell nuclei in several variations and the resulting cellular response was assessed. The focused dose application resulted in an increased RBE, thus simulating high LET effects., Der Einsatz von Hoch-LET Teilchen in der Strahlentherapie bietet viele Vorteile gegenüber der konventionellen Photonenbestrahlung, da ihre inhomogene Dosisverteilung eine erhöhte relative biologische Wirksamkeit (RBW) bedingt. Diese Arbeit gibt neue Einblicke in die Abhängigkeit der RBW von der räumlichen Dosisverteilung. Hierfür wurden Niedrig-LET Protonen mit Hilfe eines Mikrostrahls in Zellkerne appliziert und die zelluläre Antwort untersucht. Die fokussierte Dosisapplikation führte zu einer erhöhten RBW, welche mit Hoch-LET Effekten vergleichbar ist.

Published
2018

13. DNA damage interactions on both nanometer and micrometer scale determine overall cellular damage

Author

Friedrich, Thomas, primary, Ilicic, Katarina, additional, Greubel, Christoph, additional, Girst, Stefanie, additional, Reindl, Judith, additional, Sammer, Matthias, additional, Schwarz, Benjamin, additional, Siebenwirth, Christian, additional, Walsh, Dietrich W. M., additional, Schmid, Thomas E., additional, Scholz, Michael, additional, and Dollinger, Günther, additional

Published
2018
Full Text
View/download PDF

14. Increased cell survival and cytogenetic integrity by spatial dose redistribution at a compact synchrotron X-ray source

Author

Burger, Karin, primary, Ilicic, Katarina, additional, Dierolf, Martin, additional, Günther, Benedikt, additional, Walsh, Dietrich W. M., additional, Schmid, Ernst, additional, Eggl, Elena, additional, Achterhold, Klaus, additional, Gleich, Bernhard, additional, Combs, Stephanie E., additional, Molls, Michael, additional, Schmid, Thomas E., additional, Pfeiffer, Franz, additional, and Wilkens, Jan J., additional

Published
2017
Full Text
View/download PDF

15. Microbeam radiation therapy at a laser-based compact synchrotron x-ray source

Author

Burger, Karin, primary, Ilicic, Katarina, additional, Hunger, Annique, additional, Dierolf, Martin, additional, Gunther, Benedikt, additional, Schmid, Ernst, additional, Walsh, Dietrich W. M., additional, Urban, Theresa, additional, Bartzsch, Stefan, additional, Radtke, Amira, additional, Eggl, Elena, additional, Achterhold, Klaus, additional, Gleich, Bernhard, additional, Combs, Stephanie E., additional, Molls, Michael, additional, Schmid, Thomas E., additional, Pfeiffer, Franz, additional, and Wilkens, Jan J., additional

Published
2017
Full Text
View/download PDF

16. Live cell imaging of mitochondria following targeted irradiation in situ reveals rapid and highly localized loss of membrane potential

Author

Walsh, Dietrich W. M., primary, Siebenwirth, Christian, additional, Greubel, Christoph, additional, Ilicic, Katarina, additional, Reindl, Judith, additional, Girst, Stefanie, additional, Muggiolu, Giovanna, additional, Simon, Marina, additional, Barberet, Philippe, additional, Seznec, Hervé, additional, Zischka, Hans, additional, Multhoff, Gabriele, additional, Schmid, Thomas E., additional, and Dollinger, Guenther, additional

Published
2017
Full Text
View/download PDF

17. Session 23: Radiation therapy III – Particle therapy II

Author

Dollinger, Günther, primary, Greubel, Christoph, additional, Reindl, Judith, additional, Girst, Stephanie, additional, Schwarz, Benjamin, additional, Siebenwirth, Christian, additional, Walsh, Dieter, additional, Ilicic, Katarina, additional, Multhoff, Gabriele, additional, Wilkens, Jan, additional, and Schmid, Thomas E., additional

Published
2017
Full Text
View/download PDF

18. Role of membrane Hsp70 in radiation sensitivity of tumor cells

Author

Murakami, Naoya, primary, Kühnel, Annett, additional, Schmid, Thomas E., additional, Ilicic, Katarina, additional, Stangl, Stefan, additional, Braun, Isabella S., additional, Gehrmann, Mathias, additional, Molls, Michael, additional, Itami, Jun, additional, and Multhoff, Gabriele, additional

Published
2015
Full Text
View/download PDF

20. Role of membrane Hsp70 in radiation sensitivity of tumor cells

Author

Naoya Murakami, Kühnel, Annett, Schmid, Thomas E., Ilicic, Katarina, Stangl, Stefan, Braun, Isabella S., Gehrmann, Mathias, Molls, Michael, Jun Itami, and Multhoff, Gabriele

Abstract

Background: The major stress-inducible heat shock protein 70 (Hsp70) is frequently overexpressed in the cytosol and integrated in the plasma membrane of tumor cells via lipid anchorage. Following stress such as non-lethal irradiation Hsp70 synthesis is up-regulated. Intracellular located Hsp70 is known to exert cytoprotective properties, however, less is known about membrane (m)Hsp70. Herein, we investigate the role of mHsp70 in the sensitivity towards irradiation in tumor sublines that differ in their cytosolic and/or mHsp70 levels. Methods: The isogenic human colon carcinoma sublines CX+ with stable high and CX− with stable low expression of mHsp70 were generated by fluorescence activated cell sorting, the mouse mammary carcinoma sublines 4 T1 (4 T1 ctrl) and Hsp70 knock-down (4 T1 Hsp70 KD) were produced using the CRISPR/Cas9 system, and the Hsp70 down-regulation in human lung carcinoma sublines H1339 ctrl/H1339 HSF-1 KD and EPLC-272H ctrl/EPLC-272H HSF-1 KD was achieved by small interfering (si)RNA against Heat shock factor 1 (HSF-1). Cytosolic and mHsp70 was quantified by Western blot analysis/ELISA and flow cytometry; double strand breaks (DSBs) and apoptosis were measured by flow cytometry using antibodies against γH2AX and real-time PCR (RT-PCR) using primers and antibodies directed against apoptosis related genes; and radiation sensitivity was determined using clonogenic cell surviving assays. Results: CX+/CX− tumor cells exhibited similar cytosolic but differed significantly in their mHsp70 levels, 4 T1 ctrl/ 4 T1 Hsp70 KD cells showed significant differences in their cytosolic and mHsp70 levels and H1339 ctrl/H1339 HSF-1 KD and EPLC-272H ctrl/EPLC-272H HSF-1 KD lung carcinoma cell sublines had similar mHsp70 but significantly different cytosolic Hsp70 levels. γH2AX was significantly up-regulated in irradiated CX− and 4 T1 Hsp70 KD with low basal mHsp70 levels, but not in their mHsp70 high expressing counterparts, irrespectively of their cytosolic Hsp70 content. After irradiation γH2AX, Caspase 3/7 and Annexin V were up-regulated in the lung carcinoma sublines, but no significant differences were observed in H1339 ctrl/H1339 HSF-1 KD, and EPLC-272H ctrl/EPLC-272H HSF-1 KD that exhibit identical mHsp70 but different cytosolic Hsp70 levels. Clonogenic cell survival was significantly lower in CX− and 4 T1 Hsp70 KD cells with low mHsp70 expression, than in CX+ and 4 T1 ctrl cells, whereas no difference in clonogenic cell survival was observed in H1339 ctrl/H1339 HSF-1 KD and EPLC-272H ctrl/ EPLC-272H HSF-1 KD sublines with identical mHsp70 but different cytosolic Hsp70 levels. Conclusion: In summary, our results indicate that mHsp70 has an impact on radiation resistance. [ABSTRACT FROM AUTHOR]

Published
2015
Full Text
View/download PDF

Catalog

Books, media, physical & digital resources
See catalog results