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Highlights • CPPV-1 was found in all females of the study, tested with qPCR. • Was found CPPV-1 in fetal/maternal tissue in the post-mortem examination. • Feline females (with and without Reproductive Failures) were negative for commonly-occurring RF viruses in the same (FIV, FeLV e FHV-1). • Females showed clinical signs only related to RF and abnormalities WBCC. • These results provide the first description of an FPV agent causing only RF-related clinical signs in queens., Although reproductive failures (RF) such as abortion, stillbirth and neonatal mortality in cats are still under researched, it is known that many RF are caused by viral agents. This research surveyed the viral agent prevalence in queens with RF. Queens were excluded from the study if their RF was caused by issues other than infection, such as genetic, traumatic, hormonal or nutritional problems, or if they had a history of RF. Blood samples from 26 pregnant females with RF were collected for complete blood counts (BCC), renal/hepatic biochemistry and glycaemic analysis. Ultrasonography was performed to evaluate gestational age and foetal viability. When possible, placentas, humours and foetal tissues were collected. Blood samples were tested by PCR and qPCR for feline leukaemia virus (FeLV), feline immunodeficiency virus (FIV), feline alphaherpesvirus 1 (FeHV-1) and carnivore protoparvovirus 1 (CPPV-1). All maternal samples were negative for FeLV, FIV and FeHV-1 and positive for CPPV-1. In addition, foetuses from one queen and three females were positive for CPPV-1 by qPCR and for feline panleukopenia virus (FPV) through DNA sequencing. The BCC and biochemistry results revealed significant neutrophilia, lymphopenia, monocytosis, and liver enzymes. These results provide the first description of an FPV agent causing only RF-related clinical signs in queens.

The present study was conducted to investigate in 20 extensive sheep farms for the seroprevalence of Corynebacterium pseudotuberculosis (n=402) and Toxoplasma gondii (n=228). Enzyme-linked immunosorbent assay (ELISA) was used for the detection of antibodies to C. pseudotuberculosis/T. gondii. It was observed that C. pseudotuberculosis showed the highest prevalence in the region (34.07%) with statistically significant presence (p

Federal Rural University of Rio de Janeiro. Institute of Veterinary Medicine. Department of Animal Parasitology. Multiuser Molecular Biology Laboratory. Serop?dica, RJ, Brazil. Funda??o Oswaldo Cruz. Oswaldo Cruz Institute. Laboratory of Cellular Microbiology. Rio de Janeiro, RJ, Brazil. Funda??o Oswaldo Cruz. Oswaldo Cruz Institute. Laboratory of Cellular Microbiology. Rio de Janeiro, RJ, Brazil. Federal Rural University of Semi-?rido. Hospital Veterinary. Rio Grande do Norte, RN, Brazil. Federal Rural University of Semi-?rido. Hospital Veterinary. Rio Grande do Norte, RN, Brazil. Federal Rural University of Semi-?rido. Hospital Veterinary. Rio Grande do Norte, RN, Brazil. National Hansen's Disease Program. Healthcare Systems Bureau. Health Resources and Services Administration. Department of Health and Humans Services. Baton Rouge, United States. National Hansen's Disease Program. Healthcare Systems Bureau. Health Resources and Services Administration. Department of Health and Humans Services. Baton Rouge, United States. National Hansen's Disease Program. Healthcare Systems Bureau. Health Resources and Services Administration. Department of Health and Humans Services. Baton Rouge, United States. Infectious Disease Research Institute. Seattle, United States. Minist?rio da Sa?de. Secretaria de Vigil?ncia em Sa?de. Instituto Evandro Chagas. Laborat?rio de Geoprocessamento. Ananindeua, PA, Brasil. Funda??o Oswaldo Cruz. Oswaldo Cruz Institute. Laboratory of Molecular Biology Applied to Mycobacteria. Rio de Janeiro, RJ, Brazil. Funda??o Oswaldo Cruz. Oswaldo Cruz Institute. Laboratory of Molecular Biology Applied to Mycobacteria. Rio de Janeiro, RJ, Brazil. Federal Rural University of Rio de Janeiro. Institute of Veterinary Medicine. Department of Animal Parasitology. Multiuser Molecular Biology Laboratory. Serop?dica, RJ, Brazil. Leprosy was recognized as a zoonotic disease, associated with nine-banded armadillos (Dasypus novemcinctus) in the Southern United States of America in 2011. In addition, there is growing evidence to support a role for armadillos in zoonotic leprosy in South America. The current study evaluated twenty specimens of the six-banded armadillo (Euphractus sexcinctus), collected from rural locations in the state of Rio Grande do Norte (RN), Brazil for evidence of infection with Mycobacterium leprae. Serum was examined using two "in-house" enzyme-linked immunosorbent assays (ELISAs) and via two commercially available (ML flow and NDO-LID?) immunochromatographic lateral flow (LF) tests, for detection of the PGL-I and/or LID-1 antigens of the bacterium. The presence of M. leprae DNA in liver tissue was examined using the multi-copy, M. leprae-specific repetitive element (RLEP), as target in conventional and nested PCR assays. Molecular and anti-PGL-I-ELISA data indicated that 20/20 (100 %) of the armadillos were infected with M. leprae. The corresponding detection levels recorded with the LF tests were 17/20 (85 %) and 16/20 (85 %), for the NDO-LID? and ML flow tests, respectively. Our results indicate that, in common with D. novemcinctus, six banded armadillos (a species hunted and reared as a food-source in some regions of Brazil, including RN), represent a potential reservoir of M. leprae and as such, their role in a possible zoonotic cycle of leprosy within Brazil warrants further investigation.

In order to investigate the prevalence of viral diseases in vaquejada horses, samples were collected from Quarter Horse and Paint Horse breeds (males and females, aged between 2 and 15 years) from rural and urban areas of Mossoró, Rio Grande do Norte, Brazil were collected. The number of samples collected and tested varied with the techniques used: 132 serum samples were tested for vesicular stomatitis (VS) and equine viral arteritis (EVA), 118 for western and eastern equine encephalomyelitis (WEE/EEE), 114 for equine herpesvirus 1 (EHV-1), and 61 for equine influenza (EI). The techniques used varied according to the disease studied: tests for VS (Ribeirão and Alagoas strains), EVA, EHV-1, and WEE/EEE were performed using the seroneutralization technique in Vero cell cultures, while the test for for EI type-2 (H3N8) was performed using the hemagglutination inhibition technique. The following results were obtained: 39 (29.55%) and 6 (4.54%) VS-positive horses (n=132) harbored the Alagoas and Ribeirão strains, respectively; no animal tested positive for EVA (n=132); 11.01% (n=13) and 0.84% (n=1) animals tested positive for EEE and WEE (total n=118), respectively; and 22 out of 114 (19.29%) horses tested positive for EHV-1. The history of respiratory and reproductive diseases was statistically significantly (p = 0.02) related to the presence of antibodies against EHV-1. This is the first report of the seroprevalence of viral diseases in vaquejada horses in Rio Grande do Norte., Com o objetivo de pesquisar doenças virais em equinos de vaquejada foram coletadas amostras das raças Quarto de Milha e Paint Horse, machos e fêmeas, com idade variando entre 2 e 15 anos, criados nas zonas rural e urbana do município de Mossoró, Rio grande do Norte, Brasil. O número de amostras coletadas e testadas variaram durante a realização das técnicas: 132 amostras de soro foram testadas para estomatite vesicular (EV) e arterite viral equina (AVE), 118 para encefalomielite leste e oeste (EEL/EEO), 114 para herpesvírus tipo 1 (HVE-1) e 61 para influenza equina (IE). As técnicas utilizadas variaram de acordo com a enfermidade pesquisada: EV (cepas virais Ribeirão e Alagoas), AVE, HVE-1 e EEL/EEO foram pesquisados pela técnica de soroneutralização em culturas de células da linhagem Vero; enquanto a pesquisa para IE tipo-2 (H3N8) foi realizada pela técnica de inibição da hemaglutinação. Foram obtidos os seguintes resultados: 39 (29,55% cepa Alagoas) e 6 (4,54% cepa Ribeirão) de equinos positivos para EV (n=132), nenhum animal positivo para AVE (n=132), EEL/EEO (n=118) com positividade de 11,01% (n=13) e 0,84%(n=1) respectivamente; e 22 (19,29%) de equinos positivos para HVE-1 (n=114). Quanto às variáveis, histórico de doenças respiratórias e reprodutivas, as mesmas foram estatisticamente significativas (p=0.02) em relação à presença de anticorpos contra HVE-1. Este é a primeira descrição de soroprevalência de enfermidades virais em equinos de vaquejada no estado do Rio Grande do Norte, Brasil.

RESUMO Doenças infecciosas são as maiores responsáveis por falhas reprodutivas (FR) em cadelas, causando aborto, morte fetal e natimortalidade. Este estudo teve como objetivo investigar a associação entre agentes infecciosos, FR inexplicáveis e anemia em cadelas. Todas as amostras maternas e fetais foram negativas para a presença dos principais agentes infecciosos causadores de FR: herpes vírus canino 1, Neospora caninum, Brucella spp. e B. canis, enquanto agentes como o de Leishmania spp., parvovírus canino, Ehrlichia canis e Anaplasma platys foram encontrados em sangue materno. Coinfecções de A. platys/E. canis e A. platys/Leishmania spp. foram diagnosticadas. Os resultados indicam que os animais com anemia causadas por doenças transmitidas por vetores podem ser mais suscetíveis a sofrerem FR do que animais com valores hematológicos normais.

Mannheimia haemolytica (MH) is a major cause of respiratory disease in bovine complex (BRDC) that causes great economic losses. To this day, there are no reports of birds affected by this bacterium. The present report describes an atypical outbreak of mannheimiosis in captive rheas (Rhea americana). Mannheimia haemolytica (MH) é uma das principais causas da doença do complexo respiratório dos bovinos (DCRB) que causa grandes perdas econômicas. Até os dias atuais, não há relato de aves acometidas por tal bactéria. Relata-se um surto atípico de mannheimiose em emas em cativeiro (Rhea americana).

The fibroepithelial hyperplasia and cystic dysplasia correspond to feline mammary gland nonneoplastic proliferations. Although benign, they have the carcinoma as the main differential diagnosis. The study aimed to establish the clinical-epidemiological and therapeutic profile of nonneoplastic mammary neoformations of domestic cats, in order to help veterinary clinicians in the management of feline mastopathies. Information was obtained retrospectively from the Veterinary Hospital of the Semi-Arid Rural Federal University clinical records. Historical information, clinical appearance of the lesions and microscopic diagnosis were collected, besides the therapy used and the respective clinical response. The data were distributed in frequencies. It was found that most disorders (91%) corresponded to fibroepithelial hyperplasia, in contrast with one (9%) case of cystic dysplasia. Cytology was enough to confirm all cases of fibroepithelial hyperplasia, while the cystic dysplasia definitive diagnosis was obtained only by the histopathological exam. Many of the cats studied were under one year old, having not usually been ovariectomized yet, but already subjected to progestin treatment. The most common clinical sign were local cyanosis. In 90% of the female patients with breast fibroepithelial hyperplasia, primary treatment consisted of antiprogestagen drug (aglepristone) administration. After complete regression of the breast tissue, the patients underwent ovariosalpingohysterectomy. For the cystic dysplasia case, it was established the association of surgical sterilization with mastectomy. For all situations, the different therapies promoted permanent remission of the mastopathies. The data obtained become crucial for clinical management and therapy of feline patients suffering from nonneoplastic mammary gland neoformations.

Os carcinomas da mama dos felinos apresentam alta taxa de malignidade, com comportamento invasivo na maioria dos casos. Ha numerosas pesquisas sobre os tumores de mama em cadelas, porem ainda existem poucos estudos em oncologia felina, principalmente em relacao ao aspecto metastatico tumoral. O presente trabalho objetivou caracterizar o perfil de metastases associado aos carcinomas da glândula mamaria felina. Foram analisados, de forma retrospectiva, os prontuarios de 24 gatas domesticas portadoras de carcinomas da glândula mamaria. Utilizou-se o arquivo do Hospital Veterinario da Universidade Federal Rural do Semi-Arido (Mossoro, Rio Grande do Norte, Brasil), compreendendo o periodo de dezembro de 2004 a agosto de 2009. As informacoes colhidas corresponderam a distribuicao de metastases, detectadas ao exame necroscopico. Todos os disturbios sugestivos de metastases mamarias na inspecao macroscopica foram encaminhados para analise histopatologica. Houve a possibilidade de realizacao de necropsia em apenas sete (29%) pacientes. Todas as gatas demonstraram infiltracao tumoral mamaria em mais de um orgao. A avaliacao histopatologica das lesoes extramamarias revelou similaridade com os padroes dos carcinomas examinados, possibilitando assim a caracterizacao de metastatizacao, a partir das neoplasias malignas primarias das glândulas mamarias. O pulmao equivaleu ao sitio de maior comprometimento (86%), seguido da pleura parietal, coracao, linfonodos intratoracicos, figado (ambos com 43%), ossos (28,6%), diafragma, baco, rim, ovario, utero e linfonodos mesentericos caudais (ambos com 14,3%). O carcinoma mamario felino demonstra uma ampla distribuicao de disturbios secundarios para distintos orgaos e estruturas corporeas.

O objetivo do presente trabalho foi comparar a atividade cicatricial do extrato aquoso da vagem e da casca da Caesalpinia ferrea (tul.) Martius (juca) em lesoes cutâneas de asininos (Equus asinus). Para a realizacao deste trabalho, em cada um dos oito animais experimentais foram feitas quatro feridas em formato de figura plana circular, a partir de um molde metalico esterilizavel. Nas feridas do lado direito aplicou-se os extratos e nas do lado esquerdo, apenas soro fisiologico. Os animais marcados de I a IV foram tratados com extrato da casca e aqueles de V ao VIII com extrato da vagem. Foram coletados dados semanais, durante 63 dias, referentes a sinais clinicos, alem de planimetria, visando calculo da area de cicatrizacao da ferida cirurgica. Os resultados apontaram que o uso dos extratos aquosos da vagem e da casca do juca (Caesalpinia ferrea) para tratamento de feridas em asininos (Equus asinus), nas condicoes experimentais propostas, nao foi suficiente para interferir no processo de cicatrizacao.