10 results on '"Ikuyo HARA"'
Search Results
2. Distinct interactions of Sox5 and Sox10 in fate specification of pigment cells in medaka and zebrafish.
- Author
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Yusuke Nagao, Hiroyuki Takada, Motohiro Miyadai, Tomoko Adachi, Ryoko Seki, Yasuhiro Kamei, Ikuyo Hara, Yoshihito Taniguchi, Kiyoshi Naruse, Masahiko Hibi, Robert N Kelsh, and Hisashi Hashimoto
- Subjects
Genetics ,QH426-470 - Abstract
Mechanisms generating diverse cell types from multipotent progenitors are fundamental for normal development. Pigment cells are derived from multipotent neural crest cells and their diversity in teleosts provides an excellent model for studying mechanisms controlling fate specification of distinct cell types. Zebrafish have three types of pigment cells (melanocytes, iridophores and xanthophores) while medaka have four (three shared with zebrafish, plus leucophores), raising questions about how conserved mechanisms of fate specification of each pigment cell type are in these fish. We have previously shown that the Sry-related transcription factor Sox10 is crucial for fate specification of pigment cells in zebrafish, and that Sox5 promotes xanthophores and represses leucophores in a shared xanthophore/leucophore progenitor in medaka. Employing TILLING, TALEN and CRISPR/Cas9 technologies, we generated medaka and zebrafish sox5 and sox10 mutants and conducted comparative analyses of their compound mutant phenotypes. We show that specification of all pigment cells, except leucophores, is dependent on Sox10. Loss of Sox5 in Sox10-defective fish partially rescued the formation of all pigment cells in zebrafish, and melanocytes and iridophores in medaka, suggesting that Sox5 represses Sox10-dependent formation of these pigment cells, similar to their interaction in mammalian melanocyte specification. In contrast, in medaka, loss of Sox10 acts cooperatively with Sox5, enhancing both xanthophore reduction and leucophore increase in sox5 mutants. Misexpression of Sox5 in the xanthophore/leucophore progenitors increased xanthophores and reduced leucophores in medaka. Thus, the mode of Sox5 function in xanthophore specification differs between medaka (promoting) and zebrafish (repressing), which is also the case in adult fish. Our findings reveal surprising diversity in even the mode of the interactions between Sox5 and Sox10 governing specification of pigment cell types in medaka and zebrafish, and suggest that this is related to the evolution of a fourth pigment cell type.
- Published
- 2018
- Full Text
- View/download PDF
3. Sox5 functions as a fate switch in medaka pigment cell development.
- Author
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Yusuke Nagao, Takao Suzuki, Atsushi Shimizu, Tetsuaki Kimura, Ryoko Seki, Tomoko Adachi, Chikako Inoue, Yoshihiro Omae, Yasuhiro Kamei, Ikuyo Hara, Yoshihito Taniguchi, Kiyoshi Naruse, Yuko Wakamatsu, Robert N Kelsh, Masahiko Hibi, and Hisashi Hashimoto
- Subjects
Genetics ,QH426-470 - Abstract
Mechanisms generating diverse cell types from multipotent progenitors are crucial for normal development. Neural crest cells (NCCs) are multipotent stem cells that give rise to numerous cell-types, including pigment cells. Medaka has four types of NCC-derived pigment cells (xanthophores, leucophores, melanophores and iridophores), making medaka pigment cell development an excellent model for studying the mechanisms controlling specification of distinct cell types from a multipotent progenitor. Medaka many leucophores-3 (ml-3) mutant embryos exhibit a unique phenotype characterized by excessive formation of leucophores and absence of xanthophores. We show that ml-3 encodes sox5, which is expressed in premigratory NCCs and differentiating xanthophores. Cell transplantation studies reveal a cell-autonomous role of sox5 in the xanthophore lineage. pax7a is expressed in NCCs and required for both xanthophore and leucophore lineages; we demonstrate that Sox5 functions downstream of Pax7a. We propose a model in which multipotent NCCs first give rise to pax7a-positive partially fate-restricted intermediate progenitors for xanthophores and leucophores; some of these progenitors then express sox5, and as a result of Sox5 action develop into xanthophores. Our results provide the first demonstration that Sox5 can function as a molecular switch driving specification of a specific cell-fate (xanthophore) from a partially-restricted, but still multipotent, progenitor (the shared xanthophore-leucophore progenitor).
- Published
- 2014
- Full Text
- View/download PDF
4. Evolutionary differentiation of androgen receptor ohnologs is responsible for the development of androgen-dependent unique sexual characteristics in a teleost fish
- Author
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Yukiko Ogino, Satoshi Ansai, Eiji Watanabe, Masaki Yasugi, Yukitoshi Katayama, Hirotaka Sakamoto, Keigo Okamoto, Kataaki Okubo, Yasuhiro Yamamoto, Ikuyo HARA, Touko Yamazaki, Ai Kato, Yasuhiro Kamei, Kiyoshi Naruse, Kohei Ohta, Hajime Ogino, Tatsuya Sakamoto, Shinichi Miyagawa, TOMOMI SATO, Gen Yamada, Michael Baker, and Taisen Iguchi
- Abstract
Teleost fishes exhibit complex unique sexual characteristics, such as fin enlargement and courtship display, in response to androgens. However, the molecular mechanisms underlying their evolutionary acquisition remain largely unknown. To address this question, we analysed medaka (Oryzias latipes) mutants deficient in androgen receptor ohnologs (ara and arb) generated by the teleost-specific whole-genome duplication event (TSGD). We discovered that both ar ohnologs are not required for spermatogenesis and appear to be functionally redundant for courtship display in males, while both copies were necessary for their reproductive success; ara was required for tooth enlargement and behavioural attractiveness, while arb for male-specific fin morphogenesis and sexual motivation. We further showed that the differences in both the transcription of the two ars, cellular localisation of their encoded proteins and their downstream genetic programs could be responsible for the phenotypic diversity between the ara and arb mutants. These findings suggest that the ar ohnologs have diverged in the teleost lineage in two different ways: First through the loss of their roles in spermatogenesis and second through the gene duplication followed by functional differentiation that has likely resolved the pleiotropic roles derived from their ancestral gene. Thus, our results provide insights into how genome duplication impacts the massive diversification of sexual characteristics in the teleost lineage.
- Published
- 2022
5. Complex chemistry of carbon nanotubes toward efficient and stable p-type doping
- Author
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Kaho Kawasaki, Ikuyo Harada, Kouki Akaike, Qingshuo Wei, Yasuko Koshiba, Shohei Horike, and Kenji Ishida
- Subjects
Materials of engineering and construction. Mechanics of materials ,TA401-492 - Abstract
Abstract Developing efficient and stable carbon nanotube (CNT) doping techniques and elucidating their chemistry is essential for their further implementation in electronic and energy devices. Here, protonic acids and lithium salts are employed as p-type inducers and stabilizers of the doped state, respectively. Leveraging the electron-withdrawing capability of protons, protonic acids can easily induce heavily p-doped states in CNTs. Anionic species from the acids attach to the positively charged CNTs to achieve charge compensation. Introducing lithium salts with bulky, charge-delocalized anions to the p-doped CNTs results in an anion replacement driven by the free energy gain. The newly formed complexes demonstrate outstanding thermal stability in air, enduring a temperature of 100 °C for over a year. The chemical hardness of the applied anion effectively explains the difference in stability of the doped CNTs, indicating that the doping process and its stabilization can be understood and controlled through complex chemistry.
- Published
- 2024
- Full Text
- View/download PDF
6. Distinct interactions of Sox5 and Sox10 in fate specification of pigment cells in medaka and zebrafish
- Author
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Motohiro Miyadai, Yusuke Nagao, Tomoko Adachi, Kiyoshi Naruse, Yasuhiro Kamei, Masahiko Hibi, Yoshihito Taniguchi, Ikuyo Hara, Hiroyuki Takada, Hisashi Hashimoto, Ryoko Seki, and Robert N. Kelsh
- Subjects
Pigments ,0301 basic medicine ,Embryology ,Cancer Research ,Oryzias ,Epithelium ,Multipotency ,Neural Stem Cells ,Animal Cells ,Medicine and Health Sciences ,Zebrafish ,Genetics (clinical) ,Pigmentation ,SOXE Transcription Factors ,Stem Cells ,Gene Expression Regulation, Developmental ,Eukaryota ,Neural crest ,Animal Models ,Xanthophore ,Cell biology ,Phenotypes ,medicine.anatomical_structure ,Experimental Organism Systems ,Neural Crest ,Osteichthyes ,Vertebrates ,Physical Sciences ,embryonic structures ,Melanocytes ,Cellular Types ,Anatomy ,SOXD Transcription Factors ,Research Article ,Cell type ,animal structures ,lcsh:QH426-470 ,Cell Potency ,Materials Science ,SOX10 ,Melanocyte ,Biology ,Research and Analysis Methods ,03 medical and health sciences ,Model Organisms ,Developmental Neuroscience ,Genetics ,medicine ,Animals ,Cell Lineage ,Chromatophores ,Progenitor cell ,Molecular Biology ,Alleles ,Materials by Attribute ,Ecology, Evolution, Behavior and Systematics ,Embryos ,fungi ,Organisms ,Biology and Life Sciences ,Epithelial Cells ,Cell Biology ,Zebrafish Proteins ,biology.organism_classification ,Chromatophore ,lcsh:Genetics ,Fish ,Biological Tissue ,030104 developmental biology ,Genetic Loci ,Cellular Neuroscience ,human activities ,Neuroscience ,Developmental Biology - Abstract
Mechanisms generating diverse cell types from multipotent progenitors are fundamental for normal development. Pigment cells are derived from multipotent neural crest cells and their diversity in teleosts provides an excellent model for studying mechanisms controlling fate specification of distinct cell types. Zebrafish have three types of pigment cells (melanocytes, iridophores and xanthophores) while medaka have four (three shared with zebrafish, plus leucophores), raising questions about how conserved mechanisms of fate specification of each pigment cell type are in these fish. We have previously shown that the Sry-related transcription factor Sox10 is crucial for fate specification of pigment cells in zebrafish, and that Sox5 promotes xanthophores and represses leucophores in a shared xanthophore/leucophore progenitor in medaka. Employing TILLING, TALEN and CRISPR/Cas9 technologies, we generated medaka and zebrafish sox5 and sox10 mutants and conducted comparative analyses of their compound mutant phenotypes. We show that specification of all pigment cells, except leucophores, is dependent on Sox10. Loss of Sox5 in Sox10-defective fish partially rescued the formation of all pigment cells in zebrafish, and melanocytes and iridophores in medaka, suggesting that Sox5 represses Sox10-dependent formation of these pigment cells, similar to their interaction in mammalian melanocyte specification. In contrast, in medaka, loss of Sox10 acts cooperatively with Sox5, enhancing both xanthophore reduction and leucophore increase in sox5 mutants. Misexpression of Sox5 in the xanthophore/leucophore progenitors increased xanthophores and reduced leucophores in medaka. Thus, the mode of Sox5 function in xanthophore specification differs between medaka (promoting) and zebrafish (repressing), which is also the case in adult fish. Our findings reveal surprising diversity in even the mode of the interactions between Sox5 and Sox10 governing specification of pigment cell types in medaka and zebrafish, and suggest that this is related to the evolution of a fourth pigment cell type., Author summary How individual cell fates become specified from multipotent progenitors is a fundamental question in developmental and stem cell biology. Body pigment cells derive from a multipotent progenitor, but while in zebrafish there are three types of pigment cells (melanocytes, iridophores and xanthophores), in medaka these progenitors form four (as zebrafish, plus leucophores). Here, we address whether mechanisms generating each cell-type are conserved between the two species. We focus on two key regulatory proteins, Sox5 and Sox10, which we previously showed were involved in pigment cell development in medaka and zebrafish, respectively. We compare experimentally how the two proteins interact in regulating development of each of the pigment cell lineages in these fish. We show that development of all pigment cells, except leucophores, is dependent on Sox10, and that Sox5 modulates Sox10 activity antagonistically in all pigment cells in zebrafish, and melanocytes and iridophores in medaka. Surprisingly, in medaka, Sox5 acts co-operatively with Sox10 to promote xanthophore fate and to repress leucophore fate. Our findings reveal surprising diversity how Sox5 and Sox10 interact to govern pigment cell development in medaka and zebrafish, and suggest that this likely relates to the evolution of the novel leucophore pigment cell type in medaka.
- Published
- 2018
7. Sox5 Functions as a Fate Switch in Medaka Pigment Cell Development
- Author
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Yoshihito Taniguchi, Ryoko Seki, Yusuke Nagao, Takao Suzuki, Tetsuaki Kimura, Chikako Inoue, Robert N. Kelsh, Kiyoshi Naruse, Hisashi Hashimoto, Yasuhiro Kamei, Yoshihiro Omae, Masahiko Hibi, Tomoko Adachi, Ikuyo Hara, Atsushi Shimizu, Yuko Wakamatsu, and Parichy, David M.
- Subjects
Fish Proteins ,Cancer Research ,Cell type ,Embryology ,lcsh:QH426-470 ,Cellular differentiation ,Melanophores ,Oryzias ,Cell Fate Determination ,Animal Cells ,Genetics ,Animals ,Progenitor cell ,Molecular Biology ,Zebrafish ,Genetics (clinical) ,Ecology, Evolution, Behavior and Systematics ,biology ,Pigmentation ,Stem Cells ,Gene Expression Regulation, Developmental ,PAX7 Transcription Factor ,Neural crest ,Biology and Life Sciences ,Cell Differentiation ,Cell Biology ,biology.organism_classification ,Xanthophore ,Cell biology ,lcsh:Genetics ,Phenotype ,Neural Crest ,Multipotent Stem Cell ,Stem cell ,Cellular Types ,SOXD Transcription Factors ,Research Article ,Developmental Biology - Abstract
Mechanisms generating diverse cell types from multipotent progenitors are crucial for normal development. Neural crest cells (NCCs) are multipotent stem cells that give rise to numerous cell-types, including pigment cells. Medaka has four types of NCC-derived pigment cells (xanthophores, leucophores, melanophores and iridophores), making medaka pigment cell development an excellent model for studying the mechanisms controlling specification of distinct cell types from a multipotent progenitor. Medaka many leucophores-3 (ml-3) mutant embryos exhibit a unique phenotype characterized by excessive formation of leucophores and absence of xanthophores. We show that ml-3 encodes sox5, which is expressed in premigratory NCCs and differentiating xanthophores. Cell transplantation studies reveal a cell-autonomous role of sox5 in the xanthophore lineage. pax7a is expressed in NCCs and required for both xanthophore and leucophore lineages; we demonstrate that Sox5 functions downstream of Pax7a. We propose a model in which multipotent NCCs first give rise to pax7a-positive partially fate-restricted intermediate progenitors for xanthophores and leucophores; some of these progenitors then express sox5, and as a result of Sox5 action develop into xanthophores. Our results provide the first demonstration that Sox5 can function as a molecular switch driving specification of a specific cell-fate (xanthophore) from a partially-restricted, but still multipotent, progenitor (the shared xanthophore-leucophore progenitor)., Author Summary How individual cell fates are specified from multipotent progenitor cells is a fundamental question in developmental and stem cell biology. Accumulating evidence indicates that stem cells develop into each of their final, diverse cell-types after progression through one or more partially-restricted intermediates, but the molecular mechanisms underlying final fate choice are largely unknown. Neural crest cells (NCCs) give rise to diverse cell-types including multiple pigment cells and thus are a favored model for understanding the mechanism of fate specification. We have investigated how a specific fate choice is made from partially-restricted pigment cell progenitors in medaka. We show that Sry-related transcription factor Sox5 is required for fate determination between yellow xanthophore and white leucophore, and its loss causes excessive formation of leucophores and absence of xanthophores. We demonstrate that Sox5 functions cell-autonomously in the xanthophore lineage in medaka. Furthermore, pax7a is expressed in the partially-restricted progenitor cells shared with xanthophore and leucophore lineages, and Sox5 acts in some of these cells to promote xanthophore lineage. Our work reveals the role of Sox5 as a molecular switch determining xanthophore versus leucophore fate choice from the shared progenitor, and identifies an important mechanism regulating pigment cell fate choice from NCCs.
- Published
- 2014
8. Analysis of a novel gene, Sdgc, reveals sex chromosome-dependent differences of medaka germ cells prior to gonad formation
- Author
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Noriyoshi Sakai, Taro Saito, Jun Yoshimura, Shuji Shigenobu, Ikuyo Hara, Shuhei Nakamura, Tetsuaki Kimura, Amaury Herpin, Kiyoshi Naruse, Manfred Schartl, Toshiya Nishimura, Satoru Kobayashi, Tatsuya Tsukahara, Toshihiro Kawasaki, Yasuhiro Yamamoto, Minoru Tanaka, Shinichi Morishita, Laboratory of Molecular Genetics for Reproduction, National Institute for Basic Biology [Okazaki], The Graduate University for Advanced Studies, Laboratoire de Physiologie et Génomique des Poissons (LPGP), Institut National de la Recherche Agronomique (INRA)-Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique ), Department of Physiological Chemistry, Julius-Maximilians-Universität Würzburg [Wurtzbourg, Allemagne] (JMU), Interuniversity Bio-Backup Project Center, Laboratory of Bioresources, Genetic Strains Research Center, National Institute of Genetics (NIG), Department of Computational Biology, Graduate School of Frontier Sciences [Kashiwa], The University of Tokyo (UTokyo)-The University of Tokyo (UTokyo), The University of Tokyo (UTokyo), Graduate School of Frontier Sciences, Department of Biological Sciences, Graduate School of Science, Okazaki Institute for Integrative Bioscience, Funtional Genomics Facility, Grant-in-Aid for Scientific Research on Innovative Areas [22132007] Grant-in-Aid for Scientific Research (A) [25251034] to M.T. from the Ministry of Education, Culture, Sports, Science and Technology of Japan, and a grant of the Graduate Training Program of the Deutsche Forschungsgemeinschaft to M.S. [GK 1048: Molecular Basis of Organ Development in Vertebrates]., The University of Tokyo-The University of Tokyo, The University of Tokyo, and Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique )-Institut National de la Recherche Agronomique (INRA)
- Subjects
Fish Proteins ,Male ,endocrine system ,Gonad ,Somatic cell ,Genetic Linkage ,Organogenesis ,Oryzias ,Mitosis ,Locus (genetics) ,Cell Count ,Cell Separation ,Biology ,Y chromosome ,sexual identity ,Y Chromosome ,medicine ,Animals ,RNA, Messenger ,sex chromosome ,Gonads ,Molecular Biology ,Gene ,[SDV.BDD]Life Sciences [q-bio]/Development Biology ,Cells, Cultured ,Genetics ,Sex Chromosomes ,[SDV.BA]Life Sciences [q-bio]/Animal biology ,Chromosome ,Chromosome Mapping ,Gene Expression Regulation, Developmental ,[SDV.BDLR]Life Sciences [q-bio]/Reproductive Biology ,germ cell ,Up-Regulation ,medicine.anatomical_structure ,Germ Cells ,Genetic Loci ,Organ Specificity ,Female ,Germ line development ,Germ cell ,Developmental Biology ,Transcription Factors - Abstract
In vertebrates that have been examined to date, the sexual identity of germ cells is determined by the sex of gonadal somatic cells. In the teleost fish medaka, a sex-determination gene on the Y chromosome, DMY/dmrt1bY, is expressed in gonadal somatic cells and regulates the sexual identity of germ cells. Here, we report a novel mechanism by which sex chromosomes cell-autonomously confer sexually different characters upon germ cells prior to gonad formation in a genetically sex-determined species. We have identified a novel gene, Sdgc (sex chromosome-dependent differential expression in germ cells), whose transcripts are highly enriched in early XY germ cells. Chimeric analysis revealed that sexually different expression of Sdgc is controlled in a germ cell-autonomous manner by the number of Y chromosomes. Unexpectedly, DMY/dmrt1bY was expressed in germ cells prior to gonad formation, but knockdown and overexpression of DMY/dmrt1bY did not affect Sdgc expression. We also found that XX and XY germ cells isolated before the onset of DMY/dmrt1bY expression in gonadal somatic cells behaved differently in vitro and were affected by Sdgc. Sdgc maps close to the sex-determination locus, and recombination around the two loci appears to be repressed. Our results provide important insights into the acquisition and plasticity of sexual differences at the cellular level even prior to the developmental stage of sex determination.
- Published
- 2014
9. Analysis of a novel gene, Sdgc, reveals sex chromosomedependent differences of medaka germ cells prior to gonad formation.
- Author
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Toshiya Nishimura, Amaury Herpin, Tetsuaki Kimura, Ikuyo Hara, Toshihiro Kawasaki, Shuhei Nakamura, Yasuhiro Yamamoto, Saito, Taro L., Jun Yoshimura, Shinichi Morishita, Tatsuya Tsukahara, Satoru Kobayashi, Kiyoshi Naruse, Shuji Shigenobu, Noriyoshi Sakai, Schart, Manfred, and Minoru Tanaka
- Subjects
SEX chromosomes ,GENE expression ,GERM cells ,GONADAL diseases ,PHENOTYPIC plasticity - Abstract
In vertebrates that have been examined to date, the sexual identity of germ cells is determined by the sex of gonadal somatic cells. In the teleost fish medaka, a sex-determination gene on the Y chromosome, DMY/dmrt1bY, is expressed in gonadal somatic cells and regulates the sexual identity of germ cells. Here, we report a novel mechanism by which sex chromosomes cell-autonomously confer sexually different characters upon germ cells prior to gonad formation in a genetically sex determined species. We have identified a novel gene, Sdgc (sex chromosome-dependent differential expression in germ cells), whose transcripts are highly enriched in early XY germ cells. Chimeric analysis revealed that sexually different expression of Sdgc is controlled in a germ cell-autonomous manner by the number of Y chromosomes. Unexpectedly,DMY/dmrt1bYwas expressed ingerm cells prior to gonad formation, but knockdown and over expression of DMY/dmrt1bY did not affect Sdgc expression. We also found that XX and XY germ cells isolated before the onset of DMY/dmrt1bY expression in gonadal somatic cells behaved differently in vitro and were affected by Sdgc. Sdgc maps close to the sex-determination locus, and recombination around the two loci appears to be repressed. Our results provide important insights into the acquisition and plasticity of sexual differences at the cellular level even prior to the developmental stage of sex determination. [ABSTRACT FROM AUTHOR]
- Published
- 2014
- Full Text
- View/download PDF
10. The Molecular Structure of cis-4-Aza-A-homo-tetrahydro-a-santonin and trans-4- Aza- A- homo- tetrahydro-a-santonin Related to the Lactam Rule
- Author
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Hiroaki Takayanagi, Ikuyo Hara, Motoaki Goto, and Haruo Ogura
- Abstract
Stereochemistry of the titled compounds was determined by X-ray structure analysis. The seven-membered lactam rings of both compounds are in a quasi-chair conformation which agrees with the lactam rule. The mean values of the C-NH-CO-C torsion angles are -6 ° and +5°, respectively, and these values also agree with Klyne's hypothesis.
- Published
- 1989
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