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Despite the growing recognition of corpus in language teaching and learning, the importance of teaching materials informed by corpus investigation has not been widely acknowledged, especially in the EFL context. In teaching grammar, it is essential to provide authentic materials for the learners indicating both grammaticality and appropriateness. This study investigates students' perceptions of the use of corpus-informed grammar materials and the strengths and drawbacks of these materials. The participants were 40 Basic Structure students at their first-year university level. The data were collected using questionnaires and interviews after the participants attended seven meetings with Real Grammar as the book used in the classroom. The data were then coded, categorized, and analyzed to describe how the students perceived the materials. The present study showed that most students have a positive attitude toward the corpus-informed grammar book since it enables them to know the appropriate context of use related to the register (spoken or written). Corpus-informed materials have several strengths, such as describing the register-specific and frequency information, improving language learning awareness, learning motivation, critical thinking, and many more. However, corpus-informed materials should not be the only thing to rely on, so lecturers or instructors should provide adequate explanations for the materials and corpus research. These results show that it is necessary to promote the use of corpus-informed materials in EFL classrooms, including grammar classes. However, the teachers should also be trained on how corpus works, and corpus-related terms and the use of corpus-informed materials should be reconsidered for the lower level of learners.

Stance, a crucial aspect of academic writing, is complex and vital for both beginner and advanced writers. However, L2 English learners and experienced writers frequently struggle with using stance markers effectively. Currently, there remains a scarcity of research comparing the utilisation of stance markers in applied linguistics research articles between native English professional writers and L1 Indonesian speakers. This study aims to fill this gap by investigating similarities and differences in the deployment of stance markers in applied linguistics research articles by authors with L1 Indonesian and L1 English backgrounds and discussing the educational implications derived from the analysis results. The analysis involves two corpora: the Indonesian academic corpus (IAC), comprising articles authored by L1 Indonesians, and the English academic corpus (EAC), consisting of articles by native English speakers. The study investigates various types of stance markers and their distribution across the corpora, revealing significant differences in their usage. L2 English authors employ more boosters and attitude markers, while L1 English writers use more hedges and self-mentions, with the most notable disparities observed in the usage of self-references. These findings underscore the necessity for educators, particularly within the Indonesian context, to sensitise learners to the discursive norms surrounding the use of stance markers in applied linguistics research articles and provide targeted instruction to enhance English learners’ proficiency in employing stance markers effectively.

As one of the text categories, opinion texts have distinctive characteristics compared to any other texts in newspapers, including the choice of verb usage. This study then aims at preliminarily examining the verbs used in opinion articles in "The Jakarta Post" to find out the relation between frequency and text characteristics. This study collected the opinion articles of "The Jakarta Post" comprising 47.143 words. This study was assisted by "Lancsbox" to store the corpus of opinion section texts, to identify the verb lemmas, and to count the frequency of verbs. The verbs found in this study were then classified based on Scheibman's main verb classification (which is based on Halliday's and Dixon's verb types). The results of the study show that there are three most frequent verb types used in opinion texts in "The Jakarta Post;" they are "material," "verbal," and "feeling" verb types. Meanwhile, the lesser frequent ones are "perception," "possessive/relational," "relational," and "cognition" verbs types. Meanwhile, the least frequent verb types are "existential," "corporeal" and "perception/relational" verbs types. As opinion text conveys the argument of the writer, it is plausible to find "feeling" verb type belongs to the third most frequent types, along with "material" type to show concrete actions and "verbal" type to report the information. These frequencies exhibit that there is a firm relationship between text characteristics and the tendency of verb choice.

Modals are linguistic units that seem to be ubiquitous in nearly all genres and text categories. However, there are some tendencies in which some modals are more likely to occur in a particular text category than the others. It is said that modals are less frequent in academic texts compared to fiction and news. This paper then aims at describing the modals, focusing to those expressing necessity/obligation, by using corpus-based analysis. This study uses a general reference corpus, Corpus of Contemporary American English, and compiled the data from the academic subcorpus. The results show that statistically the usage frequency of necessity and obligation modals is negligible; however, no matter how small it is, it still purports to mark something. Among the modals in the same category there are some tendencies, e.g. modal should is the most frequent of all, followed by must, have to, shall, be supposed to, and have got to. The collocate analysis focuses on should and must and found out that the most frequent verb type following these two modals belong to thinking verbs (e.g., consider, learn, understand). However the most frequent modal constructions are should have and must have enabling a slightly different interpretation. Besides, due to the nature of language in an academic setting, it is thus predictable that the use of the core modals (e.g., should, must) is more frequent than the quasi-modals (e.g., have to).

Abstract Background The iroquois (iro/Irx) genes encode transcriptional regulators that belong to the TALE superclass of homeodomain proteins and have key functions during development in both vertebrates and insects. The Irx genes occur in one or two genomic clusters containing three genes each within the Drosophila and several vertebrate genomes, respectively. The similar genomic organization in Drosophila and vertebrates is widely considered as a result of convergent evolution, due to independent tandem gene duplications. In this study, we investigate the evolutionary history of the Irx genes at the scale of the whole metazoan kingdom. Results We identified in silico the putative full complement of Irx genes in the sequenced genomes of 36 different species representative of the main metazoan lineages, including non bilaterian species, several arthropods, non vertebrate chordates, and a basal vertebrate, the sea lamprey. We performed extensive phylogenetic analyses of the identified Irx genes and defined their genomic organizations. We found that, in most species, there are several Irx genes, these genes form two to four gene clusters, and the Irx genes are physically linked to a structurally and functionally unrelated gene known as CG10632 in Drosophila. Conclusion Three main conclusions can be drawn from our study. First, an Irx cluster composed of two genes, araucan/caupolican and mirror, is ancestral to the crustaceans+insects clade and has been strongly conserved in this clade. Second, three Irx genes were probably present in the last common ancestor of vertebrates and the duplication that has given rise to the six genes organized into two clusters found in most vertebrates, likely occurred in the gnathostome lineage after its separation from sea lampreys. Third, the clustered organization of the Irx genes in various evolutionary lineages may represent an exceptional case of convergent evolution or may point to the existence of an Irx gene cluster ancestral to bilaterians.

This paper examines the usage frequency of phonetically reduced modals (i.e. "gonna," "wanna," "gotta") in Present-day English. It is assumed that in distinct sociolinguistic and discourse contexts, the use of reduced modals is dynamic. To collect the data, there are five corpora used in this study, "Corpus of Contemporary American English" and "Global Web-Based English" as the representatives of Present-day English, Brown and LOB corpus as the representative of earlier years of Present-day English, and "A Representative Corpus of Historical English" to provide language data from Early Modern English to Present-day English. The analysis focuses on usage frequency of phonetically reduced modals over period of time, in different regions or countries, different medium of language use, and different text categories. The frequencies were further interpreted based on sociolinguistics and text category perspective to reveal the factors triggering the dynamic of use. The results of this study show the use of reduced modals is dramatically escalating in the last decades. According to regional observation, the use of reduced modals is more frequent in the United States than in other English-speaking countries. In relation to medium of language use, reduced modals are more commonly used in spoken language than in written language. As for text category, the usage frequency of reduced modal in fiction texts is the highest compared to academic texts and news texts. Academic texts seem to avoid these linguistic units since this sort of text must obey the use of standard language in which reduced forms are less standard and more colloquial. This phonetic reduction is plausible to occur since language system and language use apply economy principle. The use of phonetically reduced modals, however, varies in different context and is influenced by colloquialization: the more colloquial the context, the more frequent the use of reduced modals. In general, language use is phonetically simplified and sociolinguistically colloquialized.

This study aims to determine the perception of students' understanding of science material at SMP IT KHAIRUNNAS Bengkulu City during the covid-19 pandemic or during face-to-face learning. The method used in this research is using a qualitative method. The data analysis technique used is descriptive qualitative technique. The results showed that students' perceptions of science learning during the covid 19 pandemic at SMP IT KHAIRUNNAS Bengkulu City showed that on average students were more aware of learning during face-to-face learning compared to online learning, because online learning was considered difficult because the science material presented by the teacher was lacking. Obviously, students also have difficulty understanding the formula from the explanation without being written directly by the teacher on the blackboard, when face-to-face learning makes it easier to explain material and answer questions given by the teacher.

How the developmental capacity of long-lived animals copes with fluctuations in the food supply is unclear. Here, the authors show using the sea anemone Nematostella vectensis that the crosstalk between Target of Rapamycin and fibroblast growth factor signalling in ring muscles links postembryonic tentacle patterning with food availability.

As one of the text categories, opinion texts have distinctive characteristics compared to any other texts in newspapers, including the choice of verb usage. This study then aims at preliminarily examining the verbs used in opinion articles in The Jakarta Post to find out the relation between frequency and text characteristics. This study collected the opinion articles of The Jakarta Post comprising 47.143 words. This study was assisted by Lancsbox to store the corpus of opinion section texts, to identify the verb lemmas, and to count the frequency of verbs. The verbs found in this study were then classified based on Scheibman’s main verb classification (which is based on Halliday’s and Dixon’s verb types). The results of the study show that there are three most frequent verb types used in opinion texts in The Jakarta Post; they are material, verbal, and feeling verb types. Meanwhile, the lesser frequent ones are perception, possessive/relational, relational, and cognition verbs types. Meanwhile, the least frequent verb types are existential, corporeal and perception/relational verbs types. As opinion text conveys the argument of the writer, it is plausible to find feeling verb type belongs to the third most frequent types, along with material type to show concrete actions and verbal type to report the information. These frequencies exhibit that there is a firm relationship between text characteristics and the tendency of verb choice.

Stance, a crucial aspect of academic writing, is complex and vital for both beginner and advanced writers. However, L2 English learners and experienced writers frequently struggle with using stance markers effectively. Currently, there remains a scarcity of research comparing the utilisation of stance markers in applied linguistics research articles between native English professional writers and L1 Indonesian speakers. This study aims to fill this gap by investigating similarities and differences in the deployment of stance markers in applied linguistics research articles by authors with L1 Indonesian and L1 English backgrounds and discussing the educational implications derived from the analysis results. The analysis involves two corpora: the Indonesian academic corpus (IAC), comprising articles authored by L1 Indonesians, and the English academic corpus (EAC), consisting of articles by native English speakers. The study investigates various types of stance markers and their distribution across the corpora, revealing significant differences in their usage. L2 English authors employ more boosters and attitude markers, while L1 English writers use more hedges and self-mentions, with the most notable disparities observed in the usage of selfreferences. These findings underscore the necessity for educators, particularly within the Indonesian context, to sensitise learners to the discursive norms surrounding the use of stance markers in applied linguistics research articles and provide targeted instruction to enhance English learners' proficiency in employing stance markers effectively. [ABSTRACT FROM AUTHOR]

Verb is one of the most important word classes in linguistic construction due to its prominent role and dynamic nature. Interestingly, the use of verbs in different linguistic contexts might be various because the context can limit or allow certain verbs to occur more frequently than other verbs. It is compelling to study further the use of verbs in a particular linguistic context. This paper thus aims at examining the use of verbs in news section in The Jakarta Post to figure out the frequency of verbs and how it relates to the characteristics of news text. This study compiled The Jakarta Post corpus comprising news articles belong to the category of hard news from October to December 2018 with total size of 21.682 words. The verb types used in this study refer to those compiled by Scheibmann (combining Halliday’s verb taxonomy and Dixon’s verb types). Based on the analysis, it is obvious that verbal type is the most frequent verb type, followed by material and existential. As for the least frequent ones, there are corporeal and perception/relational types. It is plausible that verbal type occupies the most frequent position because the nature of news text is to deliver information and thus it needs to use verbal verbs quite often. Likewise, material verb is frequent because it states concrete action and existential verb denotes existence; both are vital in constructing news text. Meanwhile, corporeal and perception/relational types are least frequent because corporeal deals with bodily gestures actions and perception/relational shows subjectivity. Both verb types are rather insignificant concepts in news writing. Based on the results of analysis, it is obvious that there is a firm relation between frequency of verbs used in news text and the characteristics of the text: linguistic units that are not in accordance with the function of the text are not really needed and thus infrequently used.

This paper examines the usage frequency of phonetically reduced modals (i.e. gonna, wanna, gotta) in Present-day English. It is assumed that in distinct sociolinguistic and discourse contexts, the use of reduced modals is dynamic. To collect the data, there are two corpora used in this study, Corpus of Contemporary American English and Global Web-Based English as the representatives of Present-day English. The analysis focuses on usage frequency of phonetically reduced modals over period of time, in different regions, different medium of language use, and different text types. The frequencies were further interpreted based on sociolinguistics and text genre perspective to reveal the factors triggering the dynamic of use. The results of this study show the use of reduced modals is dramatically escalating in the last decades. According to regional observation, the use of reduced modals is more frequent in the United States than in other English-speaking countries. In relation to medium of language use, reduced modals are more commonly used in spoken language than in written language. As for text type, the usage frequency of reduced modal in fiction texts is the highest compared to academic texts and news texts. Academic texts seem to avoid these linguistic units since this sort of text must obey the use of standard language in which reduced forms are less standard and more colloquial. This phonetic reduction is plausible to occur since language system and language use apply economy principle. The use of phonetically reduced modals, however, varies in different context, varies in different context, influenced by colloquialization: the more colloquial the context is, the more frequent they are. In general, language use is phonetically simplified and sociolinguistically colloquialized.Â

In daily use of language, speakers need to use certain linguistic units to convey messages to the interlocutors. To assert non-factual condition, there are modality markers as parts of language elements. English grammar marks the modality concept by means of modal verbs, adverbs, adjective, conditional sentence, and the like. Regarding modal verbs, one of them is quasi-modal. This paper then examines quasi-modals in English. However, talking about English cannot be separated from its regional varieties. This research thus aims to describe the differences of quasi-modals in British English and American English. To complete the research, it attempts to reveal the factors triggering the differences.

Penutur bahasa mempunyai hakikat kreatif dan inovatif. Maka dalam pemroduksian bahasa, penutur dapat memodifikasi konstruksi lingual sedemikian rupa demi kepentingan penyampaian informasi atau pesan kepada lawan tutur. Karena bahasa-bahasa di dunia mempunyai karakteristiknya masing-masing, maka fenomena pengemasan informasi menarik untuk diamati. Oleh sebab itu, makalah ini mengulas perihal pengemasan informasi dalam bahasa Indonesia dan bahasa Inggris. Di samping itu, tulisan ini berusaha memerikan faktor-faktor internal bahasa penyebab persamaan dan perbedaan pengemasan informasi di antara kedua bahasa tersebut.

The cnidarian Nematostella vectensis has developed into a powerful model system to study the mechanisms underlying animal development, regeneration, and evolution. However, despite the significant progress in the molecular and genetic approaches in this sea anemone, endogenous protein tagging is still challenging. Here, we report a robust method for knock in for Nematostella using CRISPR/Cas9. As an outcome, we generate endogenously tagged proteins that label core molecular components of several cellular apparatus, including the nuclear envelope, cytoskeleton, cell adhesion, endoplasmic reticulum, cell trafficking, and extracellular matrix. Using live imaging, we monitor the dynamics of vesicular trafficking and endoplasmic reticulum in embryos, as well as cell contractility during the peristaltic wave of a primary polyp. This advancement in gene editing expands the molecular tool kit of Nematostella and enables experimental avenues to interrogate the cell biology of cnidarians. [ABSTRACT FROM AUTHOR]