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The relationship between milk fat intake (because of its high saturated fatty acid content) and the risk of suffering from cardiovascular diseases remains controversial. Thus, Golden Syrian hamsters were fed two types of fat—sheep milk fat that was rich in rumenic (cis9,trans11-18:2) and vaccenic (trans11-18:1) acids and olive oil—and two doses (a high- or normal-fat diet) for 14 weeks, and markers of lipid metabolism and atherosclerosis evolution were analyzed. The results revealed that the type and percentage of fat affected most plasma biochemical parameters related to lipid metabolism, while only the expression of five (CD36, SR-B1, ACAT, LDLR, and HMG-CoAR) of the studied lipid-metabolism-related genes was affected by these factors. According to aortic histology, when ingested in excess, both fats caused a similar increase in the thickness of fatty streaks, but the high-milk-fat-based diet caused a more atherogenic plasma profile. The compositions of the fats that were used, the results that were obtained, and the scientific literature indicated that the rumenic acid present in milk fat would regulate the expression of genes involved in ROS generation and, thus, protect against LDL oxidation, causing an effect similar to that of olive oil.

En Mexico ha pasado un ano en aislamiento social en casa debido a la pandemia por COVID-19. A partir de ello surgieron analisis academicos que se suman a las bases de datos oficiales respecto al sector cultural durante la pandemia. En este contexto el Observatorio de Politicas Culturales, adscrito a la Facultad de Antropologia de la Universidad Veracruzana, registro datos de julio a diciembre de 2020 a nivel local y los comparo con actividades convocadas por agentes gubernamentales y academicos. Las artes fueron las de mayor actividad, en contraste con el patrimonio tangible e intangible. Esto corresponde con la tendencia de trabajo de los gestores monitoreados en anos anteriores, pero tambien a que los agentes gubernamentales lanzaron muchas convocatorias enfocadas a esta tematica. Es necesario generar informacion constante que permita saber como y por que regresar a una vida cultural activa, para el diseno e implementacion de politicas culturales.Digitized culture, seeking strategies during health emergency by COVID-19Abstract: Mexico has spent a year in social isolation at home due to the COVID-19 pandemic. As a result, academic analyses have emerged that add to the official databases regarding the cultural sector during the pandemic. In this context, the Cultural Policies Observatory, attached to the Faculty of Anthropology of the Universidad Veracruzana, recorded data from July to December 2020 at the local level and compared them with activities organized by governmental and academic agents. The arts were the most active, in contrast to tangible and intangible heritage. This corresponds to the work trend of the managers monitored in previous years, but also to the fact that governmental agents launched many calls for proposals focused on this topic. It is necessary to generate constant information that allows us to know how and why to return to an active cultural life, for the design and implementation of cultural policies. Keywords: cultural managment; COVID-19; cultural policies; arts; heritage.

In this study a valuable fermented brewer’s spent grain (BSG) was obtained by solid state fermentation (SSF) with Rhizopus sp. and assessed for feed and food applications. SSF conditions were optimized by factorial design and response surface methodology (RSM) to maximize the value of the resulting BSG biomass. Two Rhizopus sp. strains were tested as inoculum (one wild and one mutant strain) and time and temperature were analyzed. Measured response variables included, among others, protein content, soluble protein, degree of hydrolysis, antioxidant activity, total phenolic content and antibacterial activity. Both strains led to the highest protein concentration (31.7 ± 7.6%) and soluble protein (47.4 ± 3.8 mg/g DM) when BSG was fermented at 30 °C for 9 days. The biomass obtained presented a modified amino acid profile resulting in an essential amino acid index (EAAI) of 1.58 compared to FAO human nutrition standard, with antioxidant capacity (59.7 ± 7.7% DPPH reduction) and 11 times higher total polyphenol content (2.7 ± 0.1 mg GAE/g DM). Hereby, results demonstrate that SSF of BSG results in a significant increase of highly appreciated characteristics for feed or food applications, which could lead to a promising valorization alternative.

It is estimated that between 20 and 30% of the total food produced in Europe is wasted, generating associated costs of 143 billion euros per year. These wastes include the non-eaten fraction and food chain by-products, including fish and poultry processing by-products, chitinous bioresources, agricultural, dairy, bakery, winery, and brewery by-products. Many of these wastes are rich in nutrients, even so, their high content in humidity and variability and due fundamentally to the nonexistence of an integral and efficient recovery activity causes their elimination without valorization.

Omega-3 long-chain polyunsaturated fatty acids (LC-PUFAs), such as eicosapentaenoic acid (EPA) (20:5n-3) and docosahexaenoic acid (DHA) (22:6n-3), are considered essential for human health. Microorganisms are the primary producers of omega-3 fatty acids in marine ecosystems, representing a sustainable source of these lipids, as an alternative to the fish industry. Some marine bacteria can produce LC-PUFAs de novo via the Polyunsaturated Fatty Acid (Pfa) synthase/ Polyketide Synthase (PKS) pathway, which does not require desaturation and elongation of saturated fatty acids. Cultivation-independent surveys have revealed that the diversity of microorganisms harboring a molecular marker of the pfa gene cluster (i.e., pfaA-KS domain) is high and their potential distribution in marine systems is widespread, from surface seawater to sediments. However, the isolation of PUFA producers from marine waters has been typically restricted to deep or cold environments. Here, we report a phenotypic and genotypic screening for the identification of omega-3 fatty acid producers in free-living bacterial strains isolated from 5, 500, and 1000 m deep coastal seawater from the Bay of Biscay (Spain). We further measured EPA production in pelagic Vibrio sp. strains collected at the three different depths. Vibrio sp. EPA-producers and non-producers were simultaneously isolated from the same water samples and shared a high percentage of identity in their 16S rRNA genes, supporting the view that the pfa gene cluster can be horizontally transferred. Within a cluster of EPA-producers, we found intraspecific variation in the levels of EPA synthesis for isolates harboring different genetic variants of the pfaA-KS domain. The maximum production of EPA was found in a Vibrio sp. strain isolated from a 1000 m depth (average 4.29% &plusmn, 1.07 of total fatty acids at 10 &deg, C, without any optimization of culturing conditions).

This paper examines the role of foreign direct investment (FDI) in expanding the Venezuelan oil industry in the 1990s, the drivers leading to the expropriations the following decade, and the obstacles to attracting new investments afterward. We argue that operational control by private companies, an attractive fiscal regime, and investor protection clauses were crucial elements in fostering the expansion of the oil industry after the oil opening in the 1990s. However, a successful investment cycle adding production and reserves, combined with an oil price boom, provided strong incentives for the government to increase taxes and expropriate projects. Weak institutions, policy instability, and the lack of credible property rights for the sector generated high “above ground risks” inhibiting FDI during the oil boom, despite the massive resource base and the substantial number of deals signed. FDI underperformance contributed to the decline of the Venezuelan oil industry, even before the imposition of U.S. sanctions. Using project-level data, and industry indicators, we document how volatile fiscal terms, changes in contract governance, discretionary policies, poor operating conditions, and the lack of investor protection, explain the failure to attract FDI.

The present research describes the use of Rhizopus oryzae as bioconversion agent of different agro-industrial by-products (fruit wastes, olive pulp cake, coffee grounds and potato peels) by solid state fermentation at different experimental conditions to enhance their value as animal feed. Results showed that R. oryzae grows better at fruit wastes than in other by-products. Fermentation process produced a protein and fat rich mycelia [respectively, 7.8 ± 1.2 and 9.0 ± 0.4% of dry matter (DM)] and reduced the carbohydrates content of considered by-products from 60.6 ± 0.4 to 40.1 ± 0.3% DM in 3 days. Supplementation with different nitrogen sources increases dramatically the protein concentration in the mycelia up to 46.8 ± 0.7% of DM. After fermentation, amino acid profile changed in the fermented product, with significant enrichment of several essential amino acids like valine, leucine, threonine, isoleucine and arginine and lower aspartate concentration. In conclusion, obtained product can be a high value alternative for animal feed.

Objective There has been a great interest in knowing the factors associated with progression of aortic stenosis to try to slow down this evolution. Methods We studied 283 patients with chronic ischemic heart disease recording a broad number of clinical, therapeutic and analytical variables including inflammation and mineral metabolism biomarkers. We analyzed if any of these factors is a determinant of progression to aortic stenosis, defined as an increase in maximum flow speed across the aortic valve of at least 0.5 m/s. Results 20 patients (7%) progressed to aortic stenosis in 72.4 months of follow-up. Among the patients who developed aortic stenosis, a greater age, greater percentage of peripheral arterial disease (PAD), lower treatment with beta-blockers, estimated Glomerular Filtration Rate (eGFR), total cholesterol, LDL, High-sensitivity C-reactive protein (HS-CRP), high-sensitivity troponin, N-terminal pro b-type natriuretic peptide and galectin 3 were found. The results of the multivariate analysis showed that plasma levels of Proprotein convertase subtilisin/kexin type 9 (PCSK9) [OR: 0.668 per every increase in 100.000 μg/ml CI (0.457–0.977); p=0.038], HS-CRP [OR: 1.034 per every increase in 1 mg/dl CI (1.005–1.063); p=0.022], dyslipidemia [OR: 4.622 CI (1.285–16.618); p=0.019], PAD [OR: 9.453 CI (1.703–52.452); p=0.010], and eGFR [OR: 0.962 CI (0.939–0.986); p=0.002], remained a parameter with the ability to independently predict the progression of aortic stenosis Conclusions In patients with chronic ischemic heart disease, low PCSK9 and high HS-CRP plasmatic levels, low eFGR, PAD and dyslipidemia were independent predictors of progression of aortic stenosis. More studies are needed to investigate the relationships between the progression of aortic stenosis PCSK9 and inflammation.

The present research describes an integral strategy for valorisation of fruit and vegetable discards (FVd) in feeding application, using solid-state fermentation (SSF) and submerged fermentation (SmF), for both solid and liquid fractions obtained during these by-products handle and processing, using a strain of Rhizopus sp . After SSF, fermented biomass had 1.9 times higher protein content (up to 20.2 ± 1.7% DM) than the original mass and an improved amino acid (AA) profile (45.7 ± 1.8% essential AAs). Fatty acid (FA) profile was also modified during fermentation process, with higher monounsaturated (MUFA) and lower polyunsaturated fatty acid (PUFA) percentage in the final product compared with initial substrate. Phenolic compound concentration was double in final biomass than in initial substrate (up to 8.9 ± 1.5 mg GAE/g DM) and fermented product had higher antioxidant activity (DPPH reduction of 81.3 ± 7.7% and TEAC of 3.6 ± 0.3 mg/g DM). Compared with previously reported results, fruit complementation with vegetables increased the available nitrogen and resulted in higher biomass production. The fruit and vegetable leachate (FVL) obtained by centrifugation was treated by SmF and led, per liter of substrate, to 10.6 ± 1.4 g of fungal biomass and 3.3 g protein after 7 days of fermentation. Obtained fungal biomass was rich in PUFAs (27.1 ± 7.2% of total FA) and had an AA profile comparable to soybean meal, with 45.3 ± 1.5% of essential amino acids (E AA ) . In conclusion, results demonstrate that combined solid and liquid fermentation is a successful strategy for FVd valorisation to produce valuable alternative feed ingredient due to their high protein and the well-balanced lipid content and amino acid profile.

Submerged fermentation (SmF) is an attractive biotechnological option for waste treatment, generating fungal bioprotein from food industry by-products. Using different Rhizopus sp. strains as fermentation agents, this paper describes a global strategy to identify interactions between cultivation parameters (pH 4.75–7.75, 7.5–82.5 g/l glucose, 0.75–3.75 g/l nitrogen, incubation time up to 5 days) for valorization of food industry by-products. Selected parameters and/or their interactions are critical for most of the proposed resulting values, giving the opportunity to optimize the process depending on the objective and making an “in silico” pre-evaluation of the process conditions. SmF of orange molasses leads to a high biomass and protein yield (11.9 g biomass/l and 4.0 g protein/l), with 43.1 ± 0.1% of essential amino acids and chemical oxygen demand (COD) reduction of almost 50%. Experiments with cheese whey result in 76.3 ± 1.1% of glucose consumption and a biomass production of 5.6 ± 2.2 g/l with 48.9 ± 5.1% of essential amino acids. SmF of orange molasses and cheese whey could contribute to promoting a sustainable feed industry while reducing by-product treatment.

The use of fungal cultures in modern cuisine can provide a broad number of new textures, flavors and tastes from unexpected substrates; such as fruits, vegetables and nuts. The presented research describes how Rhyzopus oryzae , a fungi used in Asian culture to produce traditional recipes, results in fruits, vegetables and grains with unique sensorial properties. Throughout the paper, different examples of these novelty uses are presented showing different examples of prototypes those have been successfully incorporated into our menus, their production procedures and their sensorial evaluations.

Bacteria of the genus Serratia, mainly S. proteamaculans and S. fonticola, are important spoilage agents in Atlantic horse mackerel (Trachurus trachurus). In order to evaluate whether bacteriophages against Serratia could delay the spoilage process, 11 viral strains active against this genus were isolated from food and best candidate was applied to fresh mackerel filets. All the phages belong to the Siphoviridae and Podoviridae families and were active at multiplicity of infection (MOI) levels below 1:1 in Long & Hammer broth. The ability of phage AZT6 to control Serratia populations in real food was tested in Atlantic horse mackerel extract and applied to fresh mackerel filets. Treatment with high phage concentration (MOI 350:1, initial Serratia population 3.9 ± 0.3 Log cfu/g) can reduce the Serratia populations up to 90% during fish storage (a maximum of 6 days) at low temperatures (6°C). Bacterial inhibition was dependent on the bacteriophage dosage, and MOI of 10:1 or lower did not significantly affect the Serratia populations.

Background Atlantic horse mackerel (Trachurus trachurus) is an important commercial fish in Europe. Changes in microbial growth, sensory quality and chemical evolution of modified atmosphere packed (MAP) horse mackerel fillets (48% CO2/50% N2/2% O2) were monitored at different storage temperatures (2, 4, 6 and 10 °C). Results The observed sensory shelf life of MAP fish fillets was found to be approximately 7 days at 2 °C, 6 days at 4 °C, 5 at 6 °C and 3 days at 10 °C. A significant correlation was observed between all sensory attributes and two groups of bacteria [total viable count (TVC) and psychrotrophic bacteria], with sensorial rejection when the bacteria approached 106 CFU g−1 at 4, 6 and 10 °C. A level of 30–50 mg total volatile basic nitrogen 100 g−1 of mackerel muscle was well correlated with sensory shelf life determination at all temperatures, except for 2 °C. During the storage time, lipid oxidation did not increase at any temperature and could not be related to quality decay. The presence of acetaldehyde and butyraldehyde in the packing atmosphere was only found in fillets stored at the highest temperature studied (10 °C). Conclusion In this study, key quality parameters were established to monitor the spoilage and shelf life of MAP Trachurus trachurus stored at different temperatures. In addition, these results confirm the use of modified atmosphere packaging as a mild preservation method for fillets of Atlantic horse mackerel. © 2012 Society of Chemical Industry

We have investigated the effect of storage temperature (0–20 °C) and carbon dioxide concentration (0–100% v/v, balance nitrogen) on the growth of Carnobacterium maltaromaticum, Serratia proteamaculans, Yersinia intermedia and Shewanella baltica, as well as on the growth of a mixed culture of the four species. These species were identified as the organisms responsible for spoilage in mackerel fillets packed under modified atmospheres in preliminary studies. The growth rates of all those groups were measured at several temperatures between 0 and 20 °C in cultures stored under air. Models describing the dependence of the maximum specific growth rate on temperature, CO2 and O2 were developed for each organism. C. maltaromaticum was the organism that showed the highest resistance to CO2 and to the lack of O2, while under aerobic condition at 0 °C. S. baltica showed the fastest growth rate. Model predictions were compared with observations on naturally contaminated horse mackerel fillets packed under modified atmospheres as well as inoculated with the bacteria strains used to generate the data for model development. These validation studies showed a good performance of models under constant and fluctuating temperature conditions. Models were implemented in a user-friendly computing tool called “Fishmap” (freely available at http://www.azti.es/en/fish-map-software.html ). This program predicts the growth of spoilage bacteria in horse mackerel fillets stored at constant and fluctuating temperature conditions under modified atmospheres and under air.

In this paper, we describe the effect of the addition of pregastric lipase on the composition and sensory properties of Idiazabal cheese. Free fatty acids (FFA), partial glycerides, free amino acids (FAA), gross composition and sensory characteristics were determined at different ripening times in cheeses manufactured with three different amounts of commercial animal lipase or with lipase-containing artisanal lamb rennet paste. The addition of lipase increased the content of total FFA, particularly of short-chain FFA, and that of total partial glycerides in cheeses. Unexpectedly, lipase utilization significantly affected total FAA concentration, which decreased in cheeses elaborated with high lipase amount. In general, Val, Glu and Leu were the major FAA, and their concentrations depended, mainly, on ripening time. Lipase addition had significant influence on the sensory characteristics of the cheeses, increasing scores for most of the flavour and odour attributes of the cheese. Principal component analysis (PCA) was done including dry matter, FFA, FAA, partial glycerides and odour and flavour attributes of the cheeses. It indicated that aroma and flavour parameters of Idiazabal cheese and the content of short-chain FFA and diglycerides were highly correlated to first principal component (PC1), while texture parameters, compositional variables and FAA were correlated to the second principal component (PC2).

Four types of lamb rennet pastes were characterized according to the sort of abomasa and the treatment it received: full fresh abomasa (FFA), full dried abomasa (FDA), empty dried abomasa (EDA), and empty frozen abomasa (EFA). These rennet pastes were studied by means of different technological parameters (milk clotting time, chymosin content, lipase activity, and aptitude against milk coagulation).The highest level of clotting activity (391.20 IMCU/g) corresponded to the rennet EDA whereas the lowest (172.87IMCU/g) was found in EFA. The chymosin content of the pastes classified them as rennet extracts, the highest chymosin level (80.46%) being found in the paste from FDA and the lowest (71.10%) being obtained from FFA.The highest level of lipase activity (10.57 U/g) was found in EDA whereas the lowest (1.46 U/g) was in paste FDA. Milk coagulation aptitude was studied through the use of a near infra-red radiation dispersion sensor. The highest value of Rmax (0.049 min-1) found in the rennet paste made with EDA indicated a higher level of casein hydrolysis and greater aggregation speed. For the same rennet at tmax value of 6.1 min was established lower than for other rennet preparations which reflected the fact that this rennet hydrolyzed 80% of the K-casein in a shorter period of time. It is concluded that the best natural lamb rennet paste of this work was FFA due to it combines appropriate proteolytic and lipolytic activities although it does not reach the maximum values for each technological parameters analyzed.

Lactic acid bacteria, such as Lactococcus lactis , are attractive hosts for the production of plant-bioactive compounds because of their food grade status, efficient expression, and metabolic engineering tools. Two genes from strawberry ( Fragaria x ananassa ), encoding an alcohol acyltransferase (SAAT) and a linalool/nerolidol synthase (FaNES), were cloned in L. lactis and actively expressed using the nisin-induced expression system. The specific activity of SAAT could be improved threefold (up to 564 pmol octyl acetate h −1 mg protein −1 ) by increasing the concentration of tRNA 1 Arg , which is a rare tRNA molecule in L. lactis . Fermentation tests with GM17 medium and milk with recombinant L. lactis strains expressing SAAT or FaNES resulted in the production of octyl acetate (1.9 μM) and linalool (85 nM) to levels above their odor thresholds in water. The results illustrate the potential of the application of L. lactis as a food grade expression platform for the recombinant production of proteins and bioactive compounds from plants.

Lipolysis during cheese ripening is usually assessed by the accumulation of free fatty acids (FFA). An assay to determine total lipolytic activity present in a cheese during ripening was established. Finely grated cheese (1 g) was directly incubated with trivalerin (204 mg) as a substrate at 35 °C for 4 h. Free valeric acid was extracted and quantified by gas chromatography. The assay was linear with time up to 24 h and up to 0.11 Lipase Units g −1 cheese. The total amount of lipolytic activity determined with this assay was consistent with the total amount of FFA present in 5 types of cheese. In Idiazabal cheese samples manufactured with or without lipase added, the total lipolytic activity determined during ripening remained constant up to 6 months of ripening. In addition to trivalerin, other possible substrates investigated were triolein, triundecanoin and the endogenous butyric acid-containing triacylglycerols present in the cheese sample. Activities measured with these substrates were considerably lower than values obtained with trivalerin due to the high levels of oleic acid present in cheese, or to difficulties in mixing triundecanoin (solid at 35 °C) with the grated cheese sample. Endogenous triacylglycerols gave increasingly lower activity values as ripening time progressed due to substrate depletion.

Three commercial lipases (lipases A1 and A2 were pregastric lipases and lipase A3 was a fungal lipase) from three different manufacturers were studied to develop the characteristic pungent flavour of Idiazabal (sheep's raw milk) cheese in experimental productions (50 L vats), both in summer and in winter. In the experimental productions, all lipases significantly increased the concentration of total free fatty acids (FFA), both after 90 and 180 days of ripening. Lipases A1 and A2 increased primarily the concentration of short-chain FFA (C4, C6 and C10), whereas C16:0 and C18:1 were the main FFA obtained with lipase A3. Cheeses made with no lipase had the lowest concentrations of total FFA, with C16:0 and C18:1 as the major FFA. The percent of short-chain FFA was 70–80% of the total for lipases A1 and A2, but 30–40% in cheeses made with lipase A3 or with no lipase. Sensory analysis was performed after 90 and 180 days of ripening. Cheeses made with lipases A1 or A2 or with lamb rennet paste (which contains pregastric lipase and was used as control for sensory analysis) had significantly higher scores in 6 of the 22 attributes analyzed (odour and flavour intensity, sharp odour, pungent flavour and rennet odour and flavour) than cheeses made with lipase A3 or with no lipase added. Lipase A1 was selected to conduct commercial cheese productions (200 L vats) made by artisan cheese makers adding 94 lipase units (LU)/50 L (high amount) or 8 LU/50 L (low amount). Control cheeses were made with lamb rennet paste having comparable amounts of lipolytic activity. After 90 and 180 days of ripening, cheeses made with the same amount of lipase (either as lipase A1 or present in lamb rennet paste) were indistinguishable from each other, both from the sensory and analytical points of view (comparable amounts of total FFA, and percent FFA composition). Those with low amount of lipase were rated as ‘mild’, whereas those with high amount of lipase were rated as ‘strong’. An industrial production (5000 L vat) with 100 LU/50 L yielded cheeses of ‘intermediate strength’ and intermediate levels of lipolysis. A linear correlation was observed between percent of short-chain FFA and the score for pungent flavour, for all amounts of lipase used in this study. We conclude that lipase A1 is an adequate commercial lipase to develop the characteristic flavour of Idiazabal cheese, both in artisan and industrial fabrications.

This work studied the addition of an adequate lipase to enhance lipolysis reactions and the development of piquant flavour and sharp odour in Idiazabal cheese, as an alternative to the use of lamb rennet paste. Cheeses were manufactured from bulk raw ewes' milk in 50 l vats with commercial bovine rennet and 80 lipase units of pregastric or 180 lipase units of fungal lipase and ripened for 180 days. A higher lipolytic activity was induced by lipase addition promoting strong changes in odour and flavour attributes. Both fungal and pregastric lipases increased the content of total free fatty acids (FFA), but the fungal lipase released mainly medium- and long-chain FFA. In contrast, the pregastric lipase preferably released short-chain FFA. Diglyceride (DG) content was considerably higher in cheeses made with added pregastric lipase compared with those made with fungal lipase or with no lipase. Monoglycerides (MG) were detected only in cheeses made with either lipase added, reaching comparable concentrations after ripening for 180 days. The cheeses made with pregastric lipase had the highest scores for odour and flavour intensity, and sharp and rennet odours, desirable attributes for the Idiazabal cheese made with lamb rennet paste. None of the texture attributes were significantly influenced by the concentrations of MG and DG in the cheeses made with either lipase. Thus, the pregastric lipase was more appropriate than the fungal lipase to develop a more traditionally-flavoured Idiazabal cheese.

A combination of conventional methods and genetic identification (PCR sequencing) was used to study the dynamics of the bacterial population during the spoilage of modified atmosphere packaged (MAP) Atlantic horse mackerel (Trachurus trachurus) fillets. The cultivable microflora in Atlantic horse mackerel samples packaged in a modified atmosphere (48% CO2, 50% N2 and 2% O2) at refrigeration temperature (6 °C) was measured on days 1, 5 and 7 using non-selective (Long and Hammer agar) and selective media (Kligler's iron agar, STAA and MRS). The microflora was genetically characterised using partial amplification of 16S rRNA gene sequences from 309 bacterial isolates obtained from Long and Hammer agar. At the end of the shelf life (5 days), the total viable counts (TVC) on Long and Hammer agar were not significantly different to the LAB counts on MRS agar (p>0.05). The molecular approach showed that Photobacterium, Arthrobacter, Chryseobacterium and Pseudoclavibacter (44.5% of total) dominated the microbial composition of the fish at the beginning of storage. However, Serratia, Shewanella and Yersinia dominated at the late spoilage stages (over 57.2% of the total). Carnobacterium was the most important species of the lactic acid bacteria (LAB) and was identified at the beginning and end of the storage period. Vibrio spp. was only found at the end of the shelf life. This research demonstrates that the microbial biodiversity in MAP Atlantic horse mackerel is enormous and the dominant species change over the storage time. The results presented here on the dominant communities in fish products will make it possible to accurately select the best preservation practices.

Atlantic horse mackerel (Trachurus trachurus) is an important commercial fish in Europe. Changes in microbial growth, sensory quality and chemical evolution of modified atmosphere packed (MAP) horse mackerel fillets (48% CO₂/50% N₂/2% O₂) were monitored at different storage temperatures (2, 4, 6 and 10 °C).The observed sensory shelf life of MAP fish fillets was found to be approximately 7 days at 2 °C, 6 days at 4 °C, 5 at 6 °C and 3 days at 10 °C. A significant correlation was observed between all sensory attributes and two groups of bacteria [total viable count (TVC) and psychrotrophic bacteria], with sensorial rejection when the bacteria approached 10⁶ CFU g⁻¹ at 4, 6 and 10 °C. A level of 30-50 mg total volatile basic nitrogen 100 g⁻¹ of mackerel muscle was well correlated with sensory shelf life determination at all temperatures, except for 2 °C. During the storage time, lipid oxidation did not increase at any temperature and could not be related to quality decay. The presence of acetaldehyde and butyraldehyde in the packing atmosphere was only found in fillets stored at the highest temperature studied (10 °C).In this study, key quality parameters were established to monitor the spoilage and shelf life of MAP Trachurus trachurus stored at different temperatures. In addition, these results confirm the use of modified atmosphere packaging as a mild preservation method for fillets of Atlantic horse mackerel.

Lung cancer comprises a large variety of histological subtypes with a frequent proclivity to form bone metastasis; a condition associated with dismal prognosis. To identify common mechanisms in the development of osteolytic metastasis, we systematically screened a battery of lung cancer cell lines and developed three models of non-small cell lung cancer (NSCLC) with a common proclivity to form osseous lesions, which represented different histological subtypes. Comparative analysis revealed different incidences and latency times. These differences were correlated with cell-type-specific secretion of osteoclastogenic factors, including macrophage inflammatory protein-1α, interleukin-8 and parathyroid hormone-related protein, some of which were exacerbated in conditions that mimicked tumor–stroma interactions. In addition, a distinct signature of matrix metalloproteinase (MMP) activity derived from reciprocal tumor–stroma interactions was detected for each tumor cell line. Thus, these results suggest subtle differences in the mechanisms of bone colonization for each lung cancer subtype, but share, although each to a different degree, dual MMP and osteoclastogenic activities that are differentially enhanced upon tumor–stromal interactions.

Fish larvae in aquaculture have high mortality rates due to pathogenic bacteria, especially the Vibrio species, and ineffective prophylactic strategies. Vaccination is not feasible in larvae and antibiotics have reduced efficacy against multidrug resistant bacteria. A novel approach to controlling Vibrio infections in aquaculture is needed. The potential of phage therapy to combat vibriosis in fish larvae production has not yet been examined. We describe the isolation and characterization of two bacteriophages capable of infecting pathogenic Vibrio and their application to prevent bacterial infection in fish larvae. Two groups of zebrafish larvae were infected with V. anguillarum (∼106 CFU mL-1) and one was later treated with a phage lysate (∼108 PFU mL-1). A third group was only added with phages. A fourth group received neither bacteria nor phages (fish control). Larvae mortality, after 72 h, in the infected and treated group was similar to normal levels and significantly lower than that of the infected but not treated group, indicating that phage treatment was effective. Thus, directly supplying phages to the culture water could be an effective and inexpensive approach toward reducing the negative impact of vibriosis in larviculture.

Lamb rennet pastes were prepared by the procedure most commonly used by Idiazabal cheese manufacturers. We studied the effects on their coagulating and lipolytic activities of the state of the stomach at the time of death (full of milk or empty), the amount of NaCl added, the origin of the lambs and paste storage time. Coagulating activities were generally between 155 and 363 units/g tissue. Pastes prepared from stomachs of lambs from slaughterhouse flocks had significantly higher coagulating activities than those of lambs from separate flocks. No significant decrease in coagulating activity was observed after 1 year storage at 4 °C. Chymosin represented 75–80% of the total coagulating activity with the remainder being pepsin. Rennet paste extracts with pH < 4·7 did not have increased coagulating activities when their pH was lowered to 2·0, while those with pH > 5·2 had activities 1·5-fold those before treatment. Lipase activity was higher in extracts of rennet pastes prepared using the stomachs of lambs that arrived at the slaughterhouse in the morning just prior to slaughter than in those prepared with the stomachs of lambs that had arrived on the previous evening. However, the reverse was the case for esterase activity. Activating the coagulating activity by pH cycling completely destroyed both lipolytic activities. Storage at 4 °C for > 1 year did not affect esterase activity but lipase activity decreased substantially after 4–5 months. Lipase, but not esterase, activity was responsible for the liberation of short-chain free fatty acids from ovine milk fat.