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Herbal products comprise a wide spectrum of locally, nationally or internationally commercialized commodities. As these products have an increasingly important position in healthcare systems worldwide, a detailed product quality assessment is of crucial importance. For the quality evaluation of commercial herbal products, a wide range of methods were used, from simpler, quicker, and cost-effective HPTLC, to hyphenated methods with MS or NMR, where more precise quantification or specific structural information is required. Additionally, most of the methods have been coupled with chemometric tools, such as PCA, or PDA, for the multivariate analysis of the high amount of data generated by chromatograms, electropherograms or spectra. The chemical methods have revealed the widespread presence of low or variable quality herbal products in the marketplace. The majority of analytical investigations present major, qualitative and quantitative, inter-product variations of their chemical composition, ranging from missing ingredients, to strikingly and unnaturally high concentrations of some compounds. Moreover, the inter-batch quality variations were frequently reported, as well as the presence of some undesirable substances. The chemical analysis of herbal products is a vital component to raise the overall awareness of quality in the herbal market and generate a quality driven approach. [ABSTRACT FROM AUTHOR]

Since 1996 till 2018, the global area cultivated with GM crops has increased 113-fold, making biotech crops one of the fastest adopted crop technology in the past decades. In the European Union, only two countries still cultivate one available transgenic crop event on minor hectarage. Moreover, the number of notifications for confined field trials has dramatically dropped in the last decade. All these are happening while the EU legislation on GM crops has come under severe criticism. The percentage of EU citizens concerned about the presence of GMOs in the environment has decreased from 30% (in 2002) to 19% (in 2011), while the level of concern about the use of GM ingredients in food or drinks has decreased from 63% (in 2005) to 27% (in 2019). The steadily increasing acceptance of the EU citizens of GMOs in the environment and food, as it was recorded by Eurobarometers, should additionally ease the way and support a positive change of the legal framework that regulates the GM crops' testing and commercial cultivation in the EU. [ABSTRACT FROM AUTHOR]

Humankind draws important benefits from large‐scale ecological processes termed ecosystem services, yet the status of several of them is declining. Reliable monitoring methods are essential for tracking the status of ecosystem services. Predation is the mainstay of natural pest control, a key ecosystem service. We used green plasticine caterpillars to monitor predation pressure, and to obtain baseline data on predator activity in transgenic Bt versus non‐Bt maize fields in Old and New World countries. Predation pressure was measured at ground and canopy levels using an identical, small‐plot experimental design in four European countries (Denmark, Slovakia, Romania and Italy) and Argentina. Total predation rate in maize was 11.7%d−1 (min. 7.2%d−1 in Argentina, max. 29.0%d−1 in Romania). Artificial caterpillars were attacked both by invertebrates (mostly chewing insects with 42.0% of the attack marks, and ants with 7.1%, but also predatory and parasitoid wasps, spiders and slugs), and vertebrates (small mammals 25.5%, and birds 20.2%). Total predation at ground level (15.7%d−1) was significantly higher than in maize canopies (6.0%d−1) in all countries, except Argentina. We found no significant differences between predator pressure in Bt versus non‐Bt maize plots. The artificial caterpillar method provided comparable, quantitative data on predation intensity, and proved to be suitable for monitoring natural pest control. This method usefully expands the existing toolkit by directly measuring ecological function rather than structure. [ABSTRACT FROM AUTHOR]

St. John's wort (Hypericum perforatum L.) herbal products have limited oversight, frequent off-label use, and insufficient monitoring of adverse drug reactions. Many herbal products have a long history of use, but there are rising concerns over product efficacy, safety and quality in the wake of recent cases exposing discrepancies between labelling and constituents. In this study, we use amplicon metabarcoding (AMB) to authenticate 78 Hypericum perforatum herbal products sold in the European Economic Area (EEA) and evaluate its ability to detect substitution with European Pharmacopoeia standard methods using thin-layer chromatography (TLC) and high performance liquid chromatography (HPLC). AMB was carried out in triplicate using nrITS1 and nrITS2 on an Ion Torrent PGM sequencing platform. AMB, TLC and HPLC can all detect H. perforatum in herbal products. Furthermore, AMB is sensitive in detecting incongruence between constituent species and those listed on the label. TLC and HPLC are accurate methods for authenticating presence of the target species, but have limited efficiency in detecting infrageneric substitution and do not yield any information on the other plant ingredients in these products. Random post-marketing AMB of herbal products by regulatory agencies could raise awareness among consumers of substitution and would provide an incentive to manufacturers to increase quality control from raw ingredients to products. [ABSTRACT FROM AUTHOR]

Introduction: Herbal medicines play an important role globally in the health care sector and in industrialised countries they are often considered as an alternative to mono-substance medicines. Current quality and authentication assessment methods rely mainly on morphology and analytical phytochemistry-based methods detailed in pharmacopoeias. Herbal products however are often highly processed with numerous ingredients, and even if these analytical methods are accurate for quality control of specific lead or marker compounds, they are of limited suitability for the authentication of biological ingredients., Objective: To review the benefits and limitations of DNA barcoding and metabarcoding in complementing current herbal product authentication., Method: Recent literature relating to DNA based authentication of medicinal plants, herbal medicines and products are summarised to provide a basic understanding of how DNA barcoding and metabarcoding can be applied to this field., Results: Different methods of quality control and authentication have varying resolution and usefulness along the value chain of these products. DNA barcoding can be used for authenticating products based on single herbal ingredients and DNA metabarcoding for assessment of species diversity in processed products, and both methods should be used in combination with appropriate hyphenated chemical methods for quality control., Conclusions: DNA barcoding and metabarcoding have potential in the context of quality control of both well and poorly regulated supply systems. Standardisation of protocols for DNA barcoding and DNA sequence-based identification are necessary before DNA-based biological methods can be implemented as routine analytical approaches and approved by the competent authorities for use in regulated procedures. © 2017 The Authors. Phytochemical Analysis Published by John Wiley & Sons Ltd., (© 2017 The Authors. Phytochemical Analysis Published by John Wiley & Sons Ltd.)