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195 results on '"Ichikawa‐Seki, Madoka"'

1. Far-East Asian Toxoplasma isolates share ancestry with North and South/Central American recombinant lineages

Author

Ihara, Fumiaki, Kyan, Hisako, Takashima, Yasuhiro, Ono, Fumiko, Hayashi, Kei, Matsuo, Tomohide, Igarashi, Makoto, Nishikawa, Yoshifumi, Hikosaka, Kenji, Sakamoto, Hirokazu, Nakamura, Shota, Motooka, Daisuke, Yamauchi, Kiyoshi, Ichikawa-Seki, Madoka, Fukumoto, Shinya, Sasaki, Motoki, Ikadai, Hiromi, Kusakisako, Kodai, Ohari, Yuma, Yoshida, Ayako, Sasai, Miwa, Grigg, Michael E., and Yamamoto, Masahiro

Published
2024
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3. Sex-inducing effects toward planarians widely present among parasitic flatworms

Author

Sekii, Kiyono, Miyashita, Soichiro, Yamaguchi, Kentaro, Saito, Ikuma, Saito, Yuria, Manta, Sayaka, Ishikawa, Masaki, Narita, Miyu, Watanabe, Taro, Ito, Riku, Taguchi, Mizuki, Furukawa, Ryohei, Ikeuchi, Aoi, Matsuo, Kayoko, Kurita, Goro, Kumagai, Takashi, Shirakashi, Sho, Ogawa, Kazuo, Sakamoto, Kimitoshi, Koyanagi, Ryo, Satoh, Noriyuki, Sasaki, Mizuki, Maezawa, Takanobu, Ichikawa-Seki, Madoka, and Kobayashi, Kazuya

Published
2023
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13. The first genetic characterization of Setaria marshalli (Nematoda, Spirurida) with reliable DNA barcoding based on a mitochondrial genetic marker

Author

Kitajima Chihiro, Ichijo Toshihiro, and Ichikawa-Seki Madoka

Subjects
setaria marshalli, filarial nematode, morphology, dna barcode, coi, Infectious and parasitic diseases, RC109-216
Abstract

Setaria marshalli is a mosquito-borne filarial nematode that causes infection in calves younger than two years old. In the present study, nematodes were obtained from a calf in Japan and morphologically identified as S. marshalli. Additionally, the partial cytochrome oxidase subunit I (COI) region (596 bp) was analyzed for the first time to establish a reliable DNA barcode. Nucleotide sequences of COI were identical among the seven worms obtained. The COI region can be a useful marker for species discrimination in the case of S. marshalli since nucleotide variations observed between the closest congener, Setaria cervi (51/596 bp), were sufficient to allow species discrimination. However, the phylogenetic relationship of S. marshalli with its congeners was unclear in a maximum likelihood tree. We found that the partial COI sequence of S. marshalli analyzed in the present study matched a relevant section of the complete mitochondrial genome of S. labiatopapillosa that was deposited in the International Nucleotide Sequence Database. This finding suggests that S. marshalli was misdiagnosed as S. labiatopapillosa in a previous study. It is crucial to conduct accurate morphological analyses to obtain reliable molecular information regarding Setaria nematodes.

Published
2022
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21. Reappraisal of Hydatigera taeniaeformis (Batsch, 1786) (Cestoda: Taeniidae) sensu lato with description of Hydatigera kamiyai n. sp.

Author

Lavikainen, Antti, Iwaki, Takashi, Haukisalmi, Voitto, Konyaev, Sergey V., Casiraghi, Maurizio, Dokuchaev, Nikolai E., Galimberti, Andrea, Halajian, Ali, Henttonen, Heikki, Ichikawa-Seki, Madoka, Itagaki, Tadashi, Krivopalov, Anton V., Meri, Seppo, Morand, Serge, Näreaho, Anu, Olsson, Gert E., Ribas, Alexis, Terefe, Yitagele, and Nakao, Minoru

Published
2016
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29. Additional file 1 of Development of novel DNA marker for species discrimination of Fasciola flukes based on the fatty acid binding protein type I gene

Author

Okamoto, Emi, Tashiro, Michiyo, Ortiz, Pedro, Mohanta, Uday Kumar, Hobán, Cristian, Murga-Moreno, César A., Angulo-Tisoc, José M., and Ichikawa-Seki, Madoka

Abstract

Additional file 1: Table S1. Fatty acid binding protein type I (FABP type I) genotypes of Fasciola flukes used in the present study.

Published
2022
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31. Assessment of genetic markers for multilocus sequence typing (MLST) of Fasciola isolates from Iran.

Author

Nazari, Naser, Rokni, Mohamad Bagher, Ichikawa‐Seki, Madoka, Raeghi, Saber, Hajjaran, Homa, Falahi, Shahab, Hamzavi, Yazdan, Heydarian, Peyman, Davari, Afshin, Ghadiri, Keyphobad, and Bozorgomid, Arezoo

Subjects
FASCIOLA, GENETIC markers, FASCIOLA hepatica, GENETIC variation, HAPLOTYPES, GENE amplification
Abstract

Background: Several markers have been described to characterise the population structure and genetic diversity of Fasciola species (Fasciola hepatica(F. hepatica) and Fasciola gigantica (F. gigantica). However, sequence analysis of a single genomic locus cannot provide sufficient resolution for the genetic diversity of the Fasciola parasite whose genomes are ∼1.3 GB in size. Objectives: To gain a better understanding of the gene diversity of Fasciola isolates from western Iran and to identify the most informative markers as candidates for epidemiological studies, five housekeeping genes were evaluated using a multilocus sequence typing (MLST) approach. Methods: MLST analysis was developed based on five genes (ND1, Pepck, Pold, Cyt b and HSP70) after genomic DNA extraction, amplification and sequencing. Nucleotide diversity and phylogeny analysis were conducted on both concatenated MLST loci and each individual locus. A median joining haplotype network was created to examine the haplotypes relationship among Fasciola isolates. Results: Thirty‐three Fasciola isolates (19 F. hepatica and 14 F. gigantica) were included in the study. A total of 2971 bp was analysed for each isolate and 31 sequence types (STs) were identified among the 33 isolates (19 for F. hepatica and 14 for F. gigantica isolates). The STs produced 44 and 42 polymorphic sites and 17 and 14 haplotypes for F. hepatica and F. gigantica, respectively. Haplotype diversity was 0.982 ± 0.026 and 1.000 ± 0.027 and nucleotide diversity was 0.00200 and 0.00353 ± 0.00088 for F. hepatica and F. gigantica, respectively. There was a high degree of genetic diversity with a Simpson's index of diversity of 0.98 and 1 for F. hepatica and F. gigantica, respectively. While HSP70 and Pold haplotypes from Fasciola species were separated by one to three mutational steps, the haplotype networks of ND1 and Cyt b were more complex and numerous mutational steps were found, likely due to recombination. Conclusions: Although HSP70 and Pold genes from F. gigantica were invariant over the entire region of sequence coverage, MLST was useful for investigating the phylogenetic relationship of Fasciola species. The present study also provided insight into markers more suitable for phylogenetic studies and the genetic structure of Fasciola parasites. [ABSTRACT FROM AUTHOR]

Published
2023
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34. Leptospiral meningoencephalitis in a raccoon dog

Author

Yamashita, Risako, primary, Yoshida, Toshinori, additional, Kobayashi, Mio, additional, Uomoto, Suzuka, additional, Shimizu, Saori, additional, Takesue, Keisuke, additional, Maeda, Natsuno, additional, Hara, Erika, additional, Ohshima, Kanami, additional, Zeng, Wen, additional, Takahashi, Yasunori, additional, Ikeuchi, Aoi, additional, Okamoto, Emi, additional, Sugiyama, Takutoshi, additional, Nagakubo, Hiroki, additional, Ichikawa-Seki, Madoka, additional, and Shibutani, Makoto, additional

Published
2021
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40. sj-pdf-1-vdi-10.1177_10406387211033583 – Supplemental material for Leptospiral meningoencephalitis in a raccoon dog

Author

Yamashita, Risako, Yoshida, Toshinori, Kobayashi, Mio, Uomoto, Suzuka, Shimizu, Saori, Takesue, Keisuke, Maeda, Natsuno, Hara, Erika, Ohshima, Kanami, Zeng, Wen, Takahashi, Yasunori, Ikeuchi, Aoi, Okamoto, Emi, Sugiyama, Takutoshi, Nagakubo, Hiroki, Ichikawa-Seki, Madoka, and Shibutani, Makoto

Subjects
70706 Veterinary Medicine, FOS: Clinical medicine, FOS: Veterinary sciences, 111599 Pharmacology and Pharmaceutical Sciences not elsewhere classified
Abstract

Supplemental material, sj-pdf-1-vdi-10.1177_10406387211033583 for Leptospiral meningoencephalitis in a raccoon dog by Risako Yamashita, Toshinori Yoshida, Mio Kobayashi, Suzuka Uomoto, Saori Shimizu, Keisuke Takesue, Natsuno Maeda, Erika Hara, Kanami Ohshima, Wen Zeng, Yasunori Takahashi, Aoi Ikeuchi, Emi Okamoto, Takutoshi Sugiyama, Hiroki Nagakubo, Madoka Ichikawa-Seki and Makoto Shibutani in Journal of Veterinary Diagnostic Investigation

Published
2021
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46. Seroprevalence of antibody to NcSAG1 antigen of Neospora caninum in cattle from Western Java, Indonesia

Author

Ichikawa-Seki, Madoka, Guswanto, Azirwan, Allamanda, Puttik, Mariamah, Euis Siti, Wibowo, Putut Eko, and Nishikawa, Yoshifumi

Subjects
cattle, Indonesia, fungi, parasitic diseases, Neospora caninum, ELISA, NcSAG1
Abstract

application/pdf, Neospora caninum can cause fetal abortion and neonatal mortality in cattle, and is a cause of economic concern worldwide. This study aimed to determine the prevalence of Neospora caninum-specific antibodies in cattle from Western Java, Indonesia. Serum samples from 991 cattle from 21 locations were tested for antibodies to N. caninum by using an enzyme-linked immunosorbent assay (ELISA) on the basis of recombinant NcSAG1. The overall seroprevalence was 16.6%, ranging from 0 to 87.5% in the sampled locations. The results of this study indicate latent infection rates of sampled animals were different in each location. Further studies are necessary to elucidate the relationship between N. caninum infection and abortion in cattle, and to identify risk factors for infection in high-prevalence environments.

Published
2016

47. Cloning and Characterization of a 2-Cys Peroxiredoxin from Babesia gibsoni

Author

MASATANI, Tatsunori, ASADA, Masahito, ICHIKAWA-SEKI, Madoka, USUI, Miho, TERKAWI, Mohamad A., HAYASHI, Kei, KAWAZU, Shin-ichiro, and XUAN, Xuenan

Subjects
Mice, Inbred ICR, Base Sequence, Reverse Transcriptase Polymerase Chain Reaction, Blotting, Western, Molecular Sequence Data, Antibodies, Protozoan, Babesia, antioxidant activity, canine, peroxiredoxin, Peroxiredoxins, Sequence Analysis, DNA, Note, Recombinant Proteins, Dogs, Babesiosis, Babesia gibsoni, Animals, Parasitology, Female, Amino Acid Sequence, Dog Diseases, Cloning, Molecular, Sequence Alignment, RNA, Protozoan
Abstract

application/pdf, Peroxiredoxins (Prxs) are a family of antioxidant enzymes. Here, we cloned a 2-Cys Prx, BgTPx-1, from the canine Babesia parasite B. gibsoni. Sequence identity between BgTPx-1 and 2-Cys Prx of B. bovis was 81% at the amino acid level. Enzyme activity assay by using recombinant BgTPx-1 (rBgTPx-1) indicated that BgTPx-1 has antioxidant activity. Antiserum from a mouse immunized with rBgTPx-1 reacted with parasite lysates and detect a protein with a monomeric size of 22 kDa and also a 44 kDa protein, which might be an inefficiently reduced dimer. BgTPx-1 was expressed in the cytoplasm of B. gibsoni merozoites. These results suggest that the BgTPx-1 may play a role to control redox balance in the cytoplasm of B. gibsoni.

Published
2014

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