17 results on '"Ibrahim Zarkesh"'
Search Results
2. Tissue-Specific Microparticles Improve Organoid Microenvironment for Efficient Maturation of Pluripotent Stem-Cell-Derived Hepatocytes
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Ensieh Zahmatkesh, Mohammad Hossein Ghanian, Ibrahim Zarkesh, Zahra Farzaneh, Majid Halvaei, Zahra Heydari, Farideh Moeinvaziri, Amnah Othman, Marc Ruoß, Abbas Piryaei, Roberto Gramignoli, Saeed Yakhkeshi, Andreas Nüssler, Mustapha Najimi, Hossein Baharvand, and Massoud Vosough
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liver organoid ,tissue-specific microparticle ,pluripotent stem cell ,hepatic differentiation ,tissue engineering ,Cytology ,QH573-671 - Abstract
Liver organoids (LOs) are receiving considerable attention for their potential use in drug screening, disease modeling, and transplantable constructs. Hepatocytes, as the key component of LOs, are isolated from the liver or differentiated from pluripotent stem cells (PSCs). PSC-derived hepatocytes are preferable because of their availability and scalability. However, efficient maturation of the PSC-derived hepatocytes towards functional units in LOs remains a challenging subject. The incorporation of cell-sized microparticles (MPs) derived from liver extracellular matrix (ECM), could provide an enriched tissue-specific microenvironment for further maturation of hepatocytes inside the LOs. In the present study, the MPs were fabricated by chemical cross-linking of a water-in-oil dispersion of digested decellularized sheep liver. These MPs were mixed with human PSC-derived hepatic endoderm, human umbilical vein endothelial cells, and mesenchymal stromal cells to produce homogenous bioengineered LOs (BLOs). This approach led to the improvement of hepatocyte-like cells in terms of gene expression and function, CYP activities, albumin secretion, and metabolism of xenobiotics. The intraperitoneal transplantation of BLOs in an acute liver injury mouse model led to an enhancement in survival rate. Furthermore, efficient hepatic maturation was demonstrated after ex ovo transplantation. In conclusion, the incorporation of cell-sized tissue-specific MPs in BLOs improved the maturation of human PSC-derived hepatocyte-like cells compared to LOs. This approach provides a versatile strategy to produce functional organoids from different tissues and offers a novel tool for biomedical applications.
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- 2021
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3. Tissue engineered scaffold fabrication methods for medical applications
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Mohammadreza Chimerad, Alireza Barazesh, Mojgan Zandi, Ibrahim Zarkesh, Armaghan Moghaddam, Pouya Borjian, Rojan Chimehrad, Alimohamad Asghari, Zeinab Akbarnejad, Hossein Ali Khonakdar, and Zohreh Bagher
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Polymers and Plastics ,General Chemical Engineering ,Analytical Chemistry - Published
- 2022
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4. Synthetic developmental biology: Engineering approaches to guide multicellular organization
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Ibrahim Zarkesh, Mohammad Kazemi Ashtiani, Zahra Shiri, Saeideh Aran, Thomas Braun, and Hossein Baharvand
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Organoids ,Tissue Engineering ,Stem Cells ,Genetics ,Cell Biology ,Biochemistry ,Developmental Biology - Abstract
Multicellular organisms of various complexities self-organize in nature. Organoids are in vitro 3D structures that display important aspects of the anatomy and physiology of their in vivo counterparts and that develop from pluripotent or tissue-specific stem cells through a self-organization process. In this review, we describe the multidisciplinary concept of "synthetic developmental biology" where engineering approaches are employed to guide multicellular organization in an experimental setting. We introduce a novel classification of engineering approaches based on the extent of microenvironmental manipulation applied to organoids. In the final section, we discuss how engineering tools might help overcome current limitations in organoid construction.
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- 2022
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5. Biofabrication of size-controlled liver microtissues incorporated with ECM-derived microparticles to prolong hepatocyte function
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Zahra Heydari, Anastasia Shpichka, Iman Akbarzadeh, Peter S. Timashev, Roberto Gramignoli, Ensieh Zahmatkesh, Massoud Vosough, Zahra Farzaneh, Abbas Piryaei, Mohammad Hossein Ghanian, Mahdokht Hossein Aghdaei, Ibrahim Zarkesh, Svetlana P. Kotova, and Hossein Baharvand
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biology ,Chemistry ,Drug discovery ,Materials Science (miscellaneous) ,Mesenchymal stem cell ,Biomedical Engineering ,Cytochrome P450 ,Industrial and Manufacturing Engineering ,Umbilical vein ,Cell biology ,Extracellular matrix ,Gene expression ,biology.protein ,Ex vivo ,Biotechnology ,Biofabrication - Abstract
Multicellular microtissues of primary human hepatocytes (PHHs) co-cultured with other supporting cell types are a promising model for drug screening and toxicological studies. However, these liver microtissues (LMs) rapidly lose their functions during ex vivo culture. Here, in order to mimic the cellular and structural hepatic microenvironment, we co-cultured PHHs with human mesenchymal stromal cells (MSCs) and human umbilical vein endothelial cells (HUVECs) in the presence of cell-sized microparticles (MPs) derived from liver extracellular matrix (LEMPs). The microwell culture platform enabled biofabrication of size-controlled multicellular microtissues (PHH:HUVEC:MSC = 3:2:1) with efficient LEMP incorporation (about 70% at a 2:1 ratio of cells:MP). The biofabricated liver microtissues (BLMs) were cultured ex vivo for 14 days and compared to the cell-only LM in terms of gene and protein expression, functional activity, cytochrome P450 (CYP450) enzyme inducibility, and drug sensitivity. The results supported superior hepatic-related gene expression, functional activity, and polarity for PHH in BLM compared to LM. CYP450 enzyme inducibility and dose-responsive sensitivity to toxic drugs were significantly higher in the BLM group. In conclusion, microtissue engineering by incorporation of tissue-specific microparticles within a multicellular microtissue can offer some advantages for drug discovery studies and cell transplantation applications. In the near future, this approach could generate a scalable platform of several functional biofabricated microtissues representing different organs.
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- 2021
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6. Smart Materials in Regenerative Medicine
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Nikoo Hossein-Khannazer, Niloufar Rezaei, Leila Montazeri, Massoud Vosough, Sara Kazemi, Iman Akbarzadeh, Moustapha Hassan, and Ibrahim Zarkesh
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Clinical microbiology ,medicine.medical_specialty ,Medical biology ,Chemistry ,medicine ,Medical physics ,Smart material ,Regenerative medicine - Published
- 2021
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7. Contributors
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Tarun Agarwal, Ibrahim N. Amirrah, C. Anandharamakrishnan, Aafreen Ansari, Mahreen Arooj, Nandika Bandara, Nabaraj Banjara, Ananya Barui, J. Basilio Heredia, Kanishka Bhunia, Samprit Bose, Rashmi Chawdhary, Rahul Chetri, Nandita Dasgupta, Shalini Dasgupta, Pallab Datta, Sangeetha Dharmalingam, Dipanjan Dwari, Chyngyz Erkinbaev, Mh Busra Fauzi, Melissa Garcia-Carrasco, Anujit Ghosal, Nikhil Gorhe, Erick P. Gutiérrez-Grijalva, Vaishnavi Hada, Hushnaara Hadem, Mehavesh Hameed, S.A.R. Hashmi, Navam Hettiarachchy, Darryl L. Holliday, Nishant Rachayya Swami Hulle, K. Jagajjanani Rao, Lily Jaiswal, Law Xia Jian, Nurkhuzaiah Kamaruzaman, Manal Khan, Tarangini Korumilli, Vaidhegi Kugarajah, Jia Xian Law, Angel Licea-Claverie, Alya Limayem, Yogeswaran Lokanathan, L. Mahalakshmi, Tapas Kumar Maiti, Sourav Maity, Zawani Mazlan, Medha Mili, Bhartendu Nath Mishra, Ahmed A. Mohamed, J.A. Moses, Soma Mukherjee, Ajay Naik, Debarshi Nath, Min Hwei Ng, Lei Nie, Sophia Devi Nongmaithem, Fatimah Mohd Nor, Atul Kumar Ojha, Abhinandan Pal, Kunal Pal, Seema Panicker, Itzel F. Parra-Aguilar, N. Prashant, Niloofar Khoshdel Rad, Mukesh Kumar Ram, Rani Puthukulangara Ramachandran, Shivendu Ranjan, Akash Roy, Sai Sateesh Sagiri, Sayandeep Saha, Atiqah Salleh, Nusaibah Sallehuddin, R. Santhosh, Angana Sarkar, Preetam Sarkar, Shiv Shankar, Ihsan Shehadi, Ali Smandri, A.K. Srivastava, Nadiah Sulaiman, Sheri-Ann Tan, Chelladurai Vellaichamy, Sarika Verma, Massoud Vosough, K.S. Yoha, Ensieh Zahmatkesh, Ibrahim Zarkesh, and Izzat Zulkiflee
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- 2022
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8. Electroconductive nanofibrillar biocomposite platforms for cardiac tissue engineering
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Tarun Agarwal, Sheri-Ann Tan, Lei Nie, Ensieh Zahmatkesh, Aafreen Ansari, Niloofar Khoshdel Rad, Ibrahim Zarkesh, Tapas Kumar Maiti, and Massoud Vosough
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- 2022
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9. Improved Differentiation of hESC-Derived Pancreatic Progenitors by Using Human Fetal Pancreatic Mesenchymal Cells in a Micro-scalable Three-Dimensional Co-culture System
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Newsha Haghparast, Yaser Tahamtani, Michael Larsen, Anne Grapin-Botton, Hamid Reza Aghayan, Babak Arjmand, Carla A. C. Gonçalves, Massoud Vosough, Mohammad Pakzad, Mahsa Zabihi, Hossein Baharvand, Zahra Ghezelayagh, Bagher Larijani, and Ibrahim Zarkesh
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Fetal mesenchyme ,TISSUES ,Mesenchyme ,Human Embryonic Stem Cells ,LINES ,Biology ,MATURATION ,CULTURE ,BETA-CELLS ,AggreWell ,medicine ,Humans ,INSULIN-PRODUCING CELLS ,Progenitor cell ,Pancreas ,Progenitor ,Matrigel ,Mesenchymal stem cell ,Cell Differentiation ,Mesenchymal Stem Cells ,EXPANSION ,Embryonic stem cell ,Coculture Techniques ,Cell biology ,ISLET ,Spheroid ,medicine.anatomical_structure ,embryonic structures ,EFFICIENT GENERATION ,Co-culture ,Stem cell ,EMBRYONIC STEM-CELLS ,Niche-specific - Abstract
Mesenchymal cells of diverse origins differ in gene and protein expression besides producing varying effects on their organ-matched epithelial cells’ maintenance and differentiation capacity. Co-culture with rodent’s tissue-specific pancreatic mesenchyme accelerates proliferation, self-renewal, and differentiation of pancreatic epithelial progenitors. Therefore, in our study, the impact of three-dimensional (3D) co-culture of human fetal pancreatic-derived mesenchymal cells (hFP-MCs) with human embryonic stem cell-derived pancreatic progenitors (hESC-PPs) development towards endocrine and beta cells was assessed. Besides, the ability to maintain scalable cultures combining hFP-MCs and hESC-PPs was investigated. hFP-MCs expressed many markers in common with bone marrow-derived mesenchymal stem cells (BM-MSCs). However, they showed higher expression of DESMIN compared to BM-MSCs. After co-culture of hESC-PPs with hFP-MCs, the pancreatic progenitor (PP) spheroids generated in Matrigel had higher expression of NGN3 and INSULIN than BM-MSCs co-culture group, which shows an inductive impact of pancreatic mesenchyme on hESC-PPs beta-cells maturation. Pancreatic aggregates generated by forced aggregation through scalable AggreWell system showed similar features compared to the spheroids. These aggregates, a combination of hFP-MCs and hESC-PPs can be applied as an appropriate tool for assessing endocrine-niche interactions and developmental processes by mimicking the pancreatic tissue.
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- 2021
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10. Fabrication of microporous inorganic microneedles by centrifugal casting method for transdermal extraction and delivery
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Mohammad-Masoud Mohebi, Ibrahim Zarkesh, Ensiyeh Hajizadeh-Saffar, Samira Gholami, Hossein Baharvand, and Mohammad Hossein Ghanian
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Materials science ,Fabrication ,Microinjections ,Biocompatibility ,Transdermal Patch ,Pharmaceutical Science ,Sintering ,Nanotechnology ,02 engineering and technology ,Administration, Cutaneous ,030226 pharmacology & pharmacy ,03 medical and health sciences ,Drug Delivery Systems ,0302 clinical medicine ,Aluminum Oxide ,Animals ,Humans ,Insulin ,Ceramic ,Skin ,Transdermal ,Hydrogels ,Microporous material ,021001 nanoscience & nanotechnology ,Rats ,Glucose ,Needles ,visual_art ,Drug delivery ,visual_art.visual_art_medium ,Particle size ,0210 nano-technology - Abstract
Microneedle patches have been widely used as transdermal transport systems because of their painless and easy application. Marked rigidity, strength, biocompatibility, and physiological stability are unique features of microneedles fabricated from ceramic materials to be used as microneedle patches. However, the conventional ceramic microneedles are typically dense structures with limited free space for biomolecule loading. A facile method is required for fabrication of biocompatible ceramic microneedles with interconnected porosity. Herein, the simple method of centrifugal casting was developed for fabrication of microporous microneedles from alumina suspensions. The slurry or resin-based alumina suspensions were casted into micromolds under centrifugal force, followed by sintering at high temperatures. The effects of particle size, solvent type, binder amount, resin content and sintering temperature on the microstructure and mechanical properties of microneedles were investigated. By optimizing the process parameters, highly porous (up to 60%) microneedles with interconnected micropores (of diameter ∼1–1.5 μm) were produced. The microporous microneedles were biocompatible and mechanically strong for skin penetration. The potential use of the microneedles for transdermal transportation of biomolecules was shown by fast and accurate extraction of glucose from a skin model and efficient loading and fast release of insulin under physiological conditions. The results suggested that the microporous alumina microneedles may serve as molecular transport systems in transdermal biosensing and drug delivery.
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- 2019
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11. Inner ear organoids: progress and outlook, with a focus on the vascularization
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Desmond A. Nunez, Hossein Baharvand, Paria Pooyan, Farideh Moeinvaziri, and Ibrahim Zarkesh
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Hearing loss ,Cell Biology ,Biology ,Organ development ,Biochemistry ,medicine.anatomical_structure ,Ear, Inner ,otorhinolaryngologic diseases ,Organoid ,medicine ,Inner ear ,Blood supply ,sense organs ,medicine.symptom ,Molecular Biology ,Neuroscience - Abstract
The inner ear is a complex organ that encodes sound, motion and orientation in space. Given the complexity of the inner ear, it is not surprising that treatments are relatively limited despite the fact that, in 2015, hearing loss was the fourth leading cause of years lived with disability (YLDs) worldwide. Inner ear organoid models are a promising tool to advance the study of multiple aspects of the inner ear to aid the development of new treatments and validate drug-based therapies. The blood supply of the inner ear plays a pivotal role in growth, maturation, and survival of inner ear tissues and their physiological functions. This vasculature cannot be ignored in order to achieve a truly in vivo-like model that mimics the microenvironment and niches of organ development. However, this aspect of organoid development has remained largely absent in the generation of inner ear organoids. The current review focuses on three-dimensional (3D) inner ear organoid and how recent technical progress in generating in vitro vasculature can enhance the next generation of these models.
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- 2021
12. Tissue-Specific Microparticles Improve Organoid Microenvironment for Efficient Maturation of Pluripotent Stem-Cell-Derived Hepatocytes
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Zahra Heydari, Mohammad Hossein Ghanian, Abbas Piryaei, Zahra Farzaneh, Saeed Yakhkeshi, Andreas K. Nussler, Majid Halvaei, Ibrahim Zarkesh, Roberto Gramignoli, Ensieh Zahmatkesh, Marc Ruoß, Mustapha Najimi, Hossein Baharvand, Farideh Moeinvaziri, Amnah Othman, Massoud Vosough, UCL - SSS/IREC/PEDI - Pôle de Pédiatrie, and UCL - (SLuc) Service de gastro-entérologie et hépatologie pédiatrique
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0301 basic medicine ,QH301-705.5 ,Human Embryonic Stem Cells ,Induced Pluripotent Stem Cells ,02 engineering and technology ,liver organoid ,Umbilical vein ,Article ,Extracellular matrix ,03 medical and health sciences ,Tissue engineering ,hepatic differentiation ,Organoid ,Human Umbilical Vein Endothelial Cells ,Animals ,Humans ,tissue-specific microparticle ,pluripotent stem cell ,Biology (General) ,Induced pluripotent stem cell ,Decellularization ,Sheep ,Chemistry ,Mesenchymal stem cell ,Cell Differentiation ,Mesenchymal Stem Cells ,General Medicine ,021001 nanoscience & nanotechnology ,Cell biology ,Transplantation ,Organoids ,030104 developmental biology ,Liver ,tissue engineering ,Hepatocytes ,0210 nano-technology - Abstract
Liver organoids (LOs) are receiving considerable attention for their potential use in drug screening, disease modeling, and transplantable constructs. Hepatocytes, as the key component of LOs, are isolated from the liver or differentiated from pluripotent stem cells (PSCs). PSC-derived hepatocytes are preferable because of their availability and scalability. However, efficient maturation of the PSC-derived hepatocytes towards functional units in LOs remains a challenging subject. The incorporation of cell-sized microparticles (MPs) derived from liver extracellular matrix (ECM), could provide an enriched tissue-specific microenvironment for further maturation of hepatocytes inside the LOs. In the present study, the MPs were fabricated by chemical cross-linking of a water-in-oil dispersion of digested decellularized sheep liver. These MPs were mixed with human PSC-derived hepatic endoderm, human umbilical vein endothelial cells, and mesenchymal stromal cells to produce homogenous bioengineered LOs (BLOs). This approach led to the improvement of hepatocyte-like cells in terms of gene expression and function, CYP activities, albumin secretion, and metabolism of xenobiotics. The intraperitoneal transplantation of BLOs in an acute liver injury mouse model led to an enhancement in survival rate. Furthermore, efficient hepatic maturation was demonstrated after ex ovo transplantation. In conclusion, the incorporation of cell-sized tissue-specific MPs in BLOs improved the maturation of human PSC-derived hepatocyte-like cells compared to LOs. This approach provides a versatile strategy to produce functional organoids from different tissues and offers a novel tool for biomedical applications.
- Published
- 2021
13. Dynamically capped hierarchically porous microneedles enable post-fabrication loading and self-regulated transdermal delivery of insulin
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Ibrahim Zarkesh, Fateme Hassan-Aghaei, Mohammad Hossein Ghanian, Samira Gholami, Hossein Baharvand, Mohammad-Masoud Mohebi, and Ensiyeh Hajizadeh-Saffar
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Fabrication ,General Chemical Engineering ,medicine.medical_treatment ,Blood sugar ,02 engineering and technology ,010402 general chemistry ,01 natural sciences ,Industrial and Manufacturing Engineering ,Chitosan ,chemistry.chemical_compound ,medicine ,Environmental Chemistry ,Transdermal ,Glucose tolerance test ,medicine.diagnostic_test ,Insulin ,technology, industry, and agriculture ,Diabetic mouse ,General Chemistry ,021001 nanoscience & nanotechnology ,0104 chemical sciences ,chemistry ,Swelling ,medicine.symptom ,0210 nano-technology ,Biomedical engineering - Abstract
Glucose-responsive microneedle (MN) patches are of great interest for painless self-regulated insulin delivery in diabetic patients to improve their quality of life. This work is aimed to develop a smart MN-based insulin delivery system with potential for post-fabrication loading of insulin and a fast, repeatable response to glucose fluctuations without sacrificing its structural constituents. A hierarchically porous inorganic MN array is developed, consisting of a macroporous alumina core to provide an external access and a large capacity for insulin loading and a mesoporous shell to control insulin release by glucose-responsive gating of its nanopores that are dynamically capped with an enzyme-loaded chitosan (CS) hydrogel. The capped MNs doff their caps to blood sugar quickly and reversibly via swelling/deswelling of the CS cap. As a result, insulin release can be repeatedly regulated during sequential cycles of hyperglycemia/normoglycemia with short intervals of 40 min. In a type 1 diabetic mouse model, application of the smart MN system quickly causes normoglycemia within 1 h, which is prolonged for 5 h. Diabetic mice treated by the smart MN system behave similarly to the healthy mice in terms of controlled blood glucose levels during the in vivo glucose tolerance test. The “cap doffing” approach can aid the development of more safe, long-lasting MN systems for smart transdermal delivery of insulin and other drugs.
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- 2021
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14. Corrigendum to 'Fabrication of microporous inorganic microneedles by centrifugal casting method for transdermal extraction and delivery' [Int. J. Pharm. 558 (2019) 299–310]
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Mohammad Hossein Ghanian, Samira Gholami, Ensiyeh Hajizadeh-Saffar, Ibrahim Zarkesh, Hossein Baharvand, and Mohammad-Masoud Mohebi
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Materials science ,Fabrication ,Chemical engineering ,Centrifugal casting (silversmithing) ,Extraction (chemistry) ,Pharmaceutical Science ,Microporous material ,Transdermal - Published
- 2021
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15. Scalable and cost-effective generation of osteogenic micro-tissues through the incorporation of inorganic microparticles within mesenchymal stem cell spheroids
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Fatemeh Bagheri, Majid Halvaei, Mohamadreza Baghaban Eslaminejad, Mohammad Hossein Ghanian, Hossein Baharvand, Mahmoud Azami, Ibrahim Zarkesh, Forough Azam Sayahpour, and Javad Mohammadi
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Osteocalcin ,0206 medical engineering ,Cell ,Biomedical Engineering ,Bioengineering ,02 engineering and technology ,Biochemistry ,Biomaterials ,Extracellular matrix ,Tissue engineering ,Osteogenesis ,medicine ,Animals ,Humans ,Rats, Wistar ,Microparticle ,Cell Proliferation ,Tissue Engineering ,Tissue Scaffolds ,Cell growth ,Chemistry ,Mesenchymal stem cell ,Bioprinting ,Spheroid ,Mesenchymal Stem Cells ,General Medicine ,021001 nanoscience & nanotechnology ,020601 biomedical engineering ,Cell aggregation ,Extracellular Matrix ,Rats ,Cell biology ,medicine.anatomical_structure ,0210 nano-technology ,Biotechnology - Abstract
Mesenchymal stem cells (MSCs) are considered primary candidates for treating complex bone defects in cell-based therapy and tissue engineering. Compared with monolayer cultures, spheroid cultures of MSCs (mesenspheres) are favorable due to their increased potential for differentiation, extracellular matrix (ECM) synthesis, paracrine activity, and in vivo engraftment. Here, we present a strategy for the incorporation of microparticles for the fabrication of osteogenic micro-tissues from mesenspheres in a cost-effective and scalable manner. A facile method was developed to synthesize mineral microparticles with cell-sized spherical shape, biphasic calcium phosphate composition (hydroxyapatite and β-tricalcium phosphate), and a microporous structure. Calcium phosphate microparticles (CMPs) were incorporated within the mesenspheres through mixing with the single cells during cell aggregation. Interestingly, the osteogenic genes were upregulated significantly (collagen type 1 (Col 1) 30-fold, osteopontin (OPN) 10-fold, and osteocalcin (OCN) 3-fold) after 14 days of culture with the incorporated CMPs, while no significant upregulation was observed with the incorporation of gelatin microparticles. The porous structure of the CMPs was exploited for loading and sustained release of an angiogenic small molecule. Dimethyloxaloylglycine (DMOG) was loaded efficiently onto the CMPs (loading efficiency: 65.32 ± 6%) and showed a sustained release profile over 12 days. Upon incorporation of the DMOG-loaded CMPs (DCMPs) within the mesenspheres, a similar osteogenic differentiation and an upregulation in angiogenic genes (VEGF 5-fold and kinase insert domain (KDR) 2-fold) were observed after 14 days of culture. These trends were also observed in immunostaining analysis. To evaluate scalable production of the osteogenic micro-tissues, the incorporation of microparticles was performed during cell aggregation in a spinner flask. The DCMPs were efficiently incorporated and directed the mesenspheres toward osteogenesis and angiogenesis. Finally, the DCMP mesenspheres were loaded within a three-dimensional printed cell trapper and transplanted into a critical-sized defect in a rat model. Computed tomography and histological analysis showed significant bone formation with blood vessel reconstruction after 8 weeks in this group. Taken together, we provide a scalable and cost-effective approach for fabrication of osteogenic micro-tissues, as building blocks of macro-tissues, that can address the large amounts of cells required for cell-based therapies.
- Published
- 2019
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16. Facile synthesis of biphasic calcium phosphate microspheres with engineered surface topography for controlled delivery of drugs and proteins
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Mahmoud Azami, Ibrahim Zarkesh, Mohammad Hossein Ghanian, Mohamadreza Baghaban Eslaminejad, Hossein Baharvand, Javad Mohammadi, and Fatemeh Bagheri
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Calcium Phosphates ,Nucleation ,chemistry.chemical_element ,Nanotechnology ,Ethylenediaminetetraacetic acid ,02 engineering and technology ,Thermal treatment ,Calcium ,010402 general chemistry ,01 natural sciences ,chemistry.chemical_compound ,Colloid and Surface Chemistry ,Drug Delivery Systems ,Tissue engineering ,Physical and Theoretical Chemistry ,Bovine serum albumin ,Edetic Acid ,biology ,Chemistry ,Surfaces and Interfaces ,General Medicine ,021001 nanoscience & nanotechnology ,Phosphate ,Microspheres ,0104 chemical sciences ,Durapatite ,Chemical engineering ,Drug delivery ,biology.protein ,0210 nano-technology ,Biotechnology - Abstract
Biphasic calcium phosphate (BCP) microspheres are of great interest due to their high stability and osteoinductive properties at specific compositions. However, the need for optimal performance at a unique composition limits their flexibility for tuning drug release by modulation of bulk properties and presents the question of engineering surface topography as an alternative. It is necessary to have a facile method to control surface topography at a defined bulk composition. Here, we have produced BCP microspheres with different surface topographies that have the capability to be used as tunable drug release systems. We synthesized calcium deficient hydroxyapatite (CDHA) microparticles by precipitating calcium and phosphate ions onto ethylenediaminetetraacetic acid (EDTA) templates. The morphology and surface topography of CDHA microparticles were controlled using process parameters, which governed nucleation and growth. These parameters included template concentration, heat rate, and stirring speed. Under low heat rate and static conditions, we could obtain spherical microparticles with long and short nanosheets on their surfaces at low and high EDTA concentrations, respectively. These nanostructured microspheres were subsequently crystallized by thermal treatment to produce EDTA-free BCP microspheres with intact morphology. These biocompatible BCP microspheres were highly effective in loading and prolonged release of both small molecule [dexamethasone (Dex)] and protein [bovine serum albumin (BSA)] models. This strategy has enabled us to control the surface topography of BCP microspheres at defined compositions and holds tremendous promise for drug delivery and tissue engineering applications.
- Published
- 2016
17. Scalable and cost-effective generation of osteogenic micro-tissues through the incorporation of inorganic microparticles within mesenchymal stem cell spheroids.
- Author
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Ibrahim Zarkesh, Majid Halvaei, Mohammad Hossein Ghanian, Fatemeh Bagheri, Forough Azam Sayahpour, Mahmoud Azami, Javad Mohammadi, Hossein Baharvand, and Mohamadreza Baghaban Eslaminejad
- Published
- 2020
- Full Text
- View/download PDF
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