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This study was conducted to evaluate the dietary effects of chromium methionine (Cr-Meth) chelate on growth performance, oxidative stress parameters, blood biochemistry, and carcass traits of broiler chickens. An experiment was conducted on 34,000 1-day-old straight-run broiler chicks (Indian River; 42.0 ± 0.03) at a commercial farm. The chicks were divided randomly into 3 groups; the first group contained 17,000 birds, which used as a control, whereas the second and third groups consisted of 7000 and 10,000 birds, respectively, with 5 replicates per group. A completely randomized design was used. The birds were fed the experimental diets containing graded levels of Cr-Meth chelate: 0 (control), 50, and 100 g/ton. This compound consisted of chromium (0.4%) chelated with methionine, and it supply the diets with 200 and 400 ppb Cr for the used levels of 50 and 100 g/ton feed, respectively. Growth performance indices (body weight, body weight gain, feed intake, and feed conversion ratio) were measured throughout the experiment. At the end of experiment, 10 birds per treatment were slaughtered, and the carcass yield with relative weight of the internal organs was determined. Also, blood samples were taken and analyzed for glutathione peroxidase activity, malondialdehyde, ALT, AST, total protein, albumin, glucose, urea, creatinine, triglycerides, and total cholesterol. It was found that Cr-Meth improved the body weight, weight gain, feed intake, and feed conversion ratio of broilers. Moreover, it reduced the mortality rate of birds. The chelated chromium can alleviate the oxidative status of birds by increasing the plasma glutathione peroxidase activity and reducing the serum malondialdehyde level. It was observed that the effects of 100 g/ton Cr-Meth chelate on performance indices, mortality rate, and oxidative stress parameters were better than that of 50 g/ton inclusion rate. Supplementation of Cr-Meth increased the total protein level, but reduced the glucose, total cholesterol, and triglyceride concentrations in the blood serum. In addition, it increased the carcass yield and reduced the abdominal fat percentage of the birds’ carcass. Therefore, chromium can be included in diets of broilers at a rate of 200 to 400 ppb, and the higher concentration was more effective than the lower one. So, it can be recommended to use Cr-Meth chelate in broiler diets at 100 g/ton to improve the productive performance and reduce the oxidative stress of birds.

The insects’ infestation decreases the nutritive value of the stored grains and causes losses in its weight, quality, or economic values. The aim of the present study was to investigate the efficiency of a natural product of clove and lemongrass extracts in controlling of red flour beetle (Tribolium castaneum) in different feedstuffs and commercial poultry diets. Different concentrations of the tested product (5, 10, 20, 50, 100 mg) were mixed with 10 g of different feedstuffs and poultry diets, and incubated at different time points. The percent repellency (PR), toxicity effect, and antifeedant activity of the plant extracts compound were assessed. The PR was detected depending on the choice method. It was found that the PR was dose and time dependent. The highest doses (50 or 100 mg/10 g feed) achieved the highest repellency effect reaching 70% at 24 h post-application (PA). The minimal PR was reported by the lowest dose of 5 mg/10 g feed reaching 50% at 24 h PA. Moreover, the PR was found to decrease by time. The toxicity effect of this natural product on T. castaneum was cumulative, not acute, in which it was recorded after 1 month of application. Besides, this effect needs high doses of the product (at dose 500 or 1000 mg/50 g feed). The used product achieved a clear antifeedant activity against T. castaneum, as the feeding deterrent index (FDI %) for corn grains was 98.5% at 1.0 and 2.0% concentration, whereas for wheat grains there was a significant difference between both concentration (96.0 vs. 74.4%). In addition, the weight loss of control corn and wheat grains was higher than the treated ones, and it was about 3.15% and 2.0% per month for corn and wheat, respectively. In conclusion, the clove and lemongrass extracts had a repellency effect reaching to 70%. Moreover, it had a lethal effect on T. castaneum. In addition, it can reduce the weight loss of the infested feeds and consequently increasing its FDI %. Therefore, the clove and lemongrass extract blend can be used to protect the feedstuffs from the damage by this insect.

This study was conducted to evaluate the effect of feeding hydroponic barley sprouts to sheep on dry matter intake, nutrient digestibility, nitrogen balance, rumen fermentation parameters and ruminal enzymes activity. The barley sprouts were prepared in a hydroponic sprouting unit under controlled environment. Barley grains were first washed, soaked for one day using tap water, and then covered with a wet towel for another one day for germination. Thereafter, they were spread out in trays and were irrigated three times daily by tap water. The trays contained green fodder were removed after 8 days and the harvested carpets were exposed to air ventilation, then weighed and shredded to small pieces before feeding to the animals. Twenty mature rams (50 ± 1.30 kg bw) were randomly distributed into four groups, each with five rams. The rams in group 1 and 2 were fed on Egyptian clover and hydroponic barley sprouts only, respectively, while the animals in group 3 and 4 were offered the same forage sources used in previous groups but with supplying a concentrate mixture. The experiment lasted for three weeks. It was found that feeding of sprouted barley alone reduced total DM intake by about 61.5% than that of the clover alone, but this effect was ameliorated when fed with the concentrate. However, the digestibility of different nutrients was increased in sprouted barley diet by an average of 10.0% compared to the clover. The nitrogen retention % was similar in rams fed barely sprouts or clover without concentrate. Total volatile fatty acids and propionate production was higher by 18.5% and 8.31%, respectively, in rumen of rams fed the sprouts alone or with concentrate than the clover alone. In addition, feeding of barley sprouts increased the total rumen protozoa count by about 31.3% and most of ruminal enzyme activities (45.0, 9.0 and 23.2% for amylase, cellulose and protease, respectively) compared to the clover. Providing the concentrate with sprouted barley or clover improved the nutrients digestibility (by about 14.0%) and nitrogen retention (by 46.0%) as well as amylase (by 60.0%) and urease (by 15.0%) activity in comparison to feeding of these ingredients alone. In conclusion, feeding of barley sprouts alone to sheep reduced the DM intake, however, it can improve the nutrients digestibility, rumen fermentation and ruminal enzyme activities. Moreover, use of sprouted barley in sheep diets could result in better digestibility and fermentation results than the clover. Feeding of concentrate with sprouted barley increased its positive effect on nutrients digestibility and rumen fermentation and improved the total DM intake. Thus, it can be recommended to feed the hydroponic barley sprouts with the concentrate to obtain an optimal DM intake as well as a high animal performance.

Three experiments were performed to evaluate the digestibility and fermentation rates of two types of lignocellulose products that can be used in diets of pigs and turkeys. Experiment I: In vitro dry matter digestibility studies using Daisy system were conducted by using fecal or excreta fluid of swine/turkeys as a source of inoculums. The feedstuffs used as substrates were commercial swine or turkey diet, lignocellulose A and B. The dry matter digestibility was found to be about 75%, 28%, and

This study was conducted to evaluate the effect of using protease in diets of Nile tilapia on growth performance, water quality, blood parameters and intestinal morphology. The cost of these diets and their return on fish performance was calculated. A total of 360 fish were randomly allocated into four groups with triplicates (30 fish per replicate). Four diets were formulated; two controls (without protease supplementation) and two experimental diets (supplemented with protease). The first control diet contained the normal protein requirement (30% CP; control +ve), while the second control had a low protein content (29% CP; control -ve). The third diet was supplemented with protease at a dose of 500 g/ton, and its CP content was reduced to 29.0%, by reducing the fish meal content. The fourth diet contained the same CP level as the first control (30%) and supplemented with 250 g protease per ton feed. The experiment lasted for 14 weeks. The results showed that body weight and length, weight gain, specific growth rate, feed intake and feed conversion efficiency in the control -ve group (low CP) supplemented with protease were similar (p > 0.05) to that of the control +ve with normal CP content. However, these performance parameters were lower (p

The insects' infestation decreases the nutritive value of the stored grains and causes losses in its weight, quality, or economic values. The aim of the present study was to investigate the efficiency of a natural product of clove and lemongrass extracts in controlling of red flour beetle (Tribolium castaneum) in different feedstuffs and commercial poultry diets. Different concentrations of the tested product (5, 10, 20, 50, 100 mg) were mixed with 10 g of different feedstuffs and poultry diets, and incubated at different time points. The percent repellency (PR), toxicity effect, and antifeedant activity of the plant extracts compound were assessed. The PR was detected depending on the choice method. It was found that the PR was dose and time dependent. The highest doses (50 or 100 mg/10 g feed) achieved the highest repellency effect reaching 70% at 24 h post-application (PA). The minimal PR was reported by the lowest dose of 5 mg/10 g feed reaching 50% at 24 h PA. Moreover, the PR was found to decrease by time. The toxicity effect of this natural product on T. castaneum was cumulative, not acute, in which it was recorded after 1 month of application. Besides, this effect needs high doses of the product (at dose 500 or 1000 mg/50 g feed). The used product achieved a clear antifeedant activity against T. castaneum, as the feeding deterrent index (FDI %) for corn grains was 98.5% at 1.0 and 2.0% concentration, whereas for wheat grains there was a significant difference between both concentration (96.0 vs. 74.4%). In addition, the weight loss of control corn and wheat grains was higher than the treated ones, and it was about 3.15% and 2.0% per month for corn and wheat, respectively. In conclusion, the clove and lemongrass extracts had a repellency effect reaching to 70%. Moreover, it had a lethal effect on T. castaneum. In addition, it can reduce the weight loss of the infested feeds and consequently increasing its FDI %. Therefore, the clove and lemongrass extract blend can be used to protect the feedstuffs from the damage by this insect.

The study was conducted to investigate the effect of low metabolizable energy diets with normal or narrow metabolizable energy to crude protein ratios (ME:CP) on performance, carcass characteristics, body composition and blood parameters in broilers fed from 1 to 42 days of age. The chicks were divided into 7 groups. The birds were fed starter & grower diets. Seven experimental diets were formulated in each phase; one control and 6 tested diets. The control diet was formulated according to the NRC of poultry (1994) and the other six diets fed three different levels of low energy diets (2900, 2700 & 2500 kcal/kg; one level for each 2 groups). The first three tested groups named ''normal calorie-protein ratio” groups in which the CP decreased in proportion to the decrease in ME, keeping the normal NRC ratio. In the second three tested groups, termed “narrow calorie-protein ratio” groups, the dietary protein was kept at the NRC levels leading to ratios narrower than that of the NRC. Results showed that chicks fed low ME diets with normal energy to protein ratio had lower body weight and feed utilization efficiency than the chicks fed the control diets. While, birds fed the low ME diets with normal protein NRC-levels and narrow ratios had nearly equal weight and feed conversion to the control. The body composition and carcass characteristics were not affected by the dietary treatments. Moreover, the blood parameters had no significant variations among the groups, except for total protein, ALT and AST which had an increased response to decreased dietary energy density. In conclusion, decreasing the dietary ME level without decreasing the crude protein level was more efficient economically and had no any adverse effect on the performance. However, decreasing of dietary ME with normal ME:CP ratio resulted in decreased performance and low economic efficiency.

Small ruminants represent an important economic source in small farm systems and agriculture. Feed is the main component of livestock farming, which has gained special attention to improve animal performance. Many studies have been done to improve feed utilisation through addition of feed additives. For a long period, antibiotics have been widely used as growth promoters in livestock diets. Due to their ban in many countries, search for alternative feed additives has been intensified. Probiotics are one of these alternatives recognised to be safe to the animals. Use of probiotics in small ruminant nutrition has been confirmed to improve animal health, productivity and immunity. Probiotics improved growth performance through enhancing of rumen microbial ecosystem, nutrient digestibility and feed conversion rate. Moreover, probiotics have been reported to stabilise rumen pH, increase volatile fatty acids production and to stimulate lactic acid utilising protozoa, resulting in a highly efficient rumen function. Furthermore, use of probiotics has been found to increase milk production and can reduce incidence of neonatal diarrhea and mortality. However, actual mechanisms through which probiotics exert these functions are not known. Since research on application of probiotics in small ruminants is scarce, the present review attempts to discuss the potential roles of this class of feed additives on productive performance and health status of these animals.

The objective of the present study was to determine the possible effect of gastrointestinal nematodes upon serum mineral concentrations of lambs. Twelve male lambs were used. Lambs were randomly assigned to 2 groups (n = 6): Group 1 infected with gastrointestinal nematodes and Group 2 as controls. Lambs of Group 1 were infected with a single dose of 15,000 L(3) larvae of GI nematodes (Haemonchus, Teladorsagia, Trichostrongylus, Cooperia and Oesophagostomum–Bunostomum). Blood samples were collected from the investigated animals individually every 2 weeks. However, the differences in serum macro-minerals (Ca, Mg, P, K, and Na) among groups were not significant. Although the differences in serum macro-minerals among groups were not significant and the iron serum concentration remained unaltered, the gastrointestinal parasitism reduced significantly/substantially the serum copper levels.

Objective This study was conducted to investigate the effects of probiotic, prebiotic and synbiotic with and without feed restriction on broilers performance, blood parameters, carcass characteristics, and feed cost of production from 1 to 56 days of age. Methods Two hundred and forty unsexed one day-old chicks of Arbor Acres breed were used. Two trials, I and II, were conducted, with 120 birds in both. Each trial was divided into 4 equal groups. The birds in trial I were fed ad libitum throughout the experiment, while the chicks in trial II were fed ad libitum during the first week of age, then subjected to 5 hours/d of feed restriction from the beginning of the second week up to the end of the experiment. In both trials, the birds in group 1 were fed on a control diet while the other groups were given the same control diet supplemented either with a probiotic in group 2, prebiotic in group 3, or synbiotic in group 4. Results It was found that chicks fed diets supplemented with probiotic, prebiotic and synbiotic (with and without feed restriction) exhibited higher body weight and feed efficiency than chicks fed the control diets. The feed additives in both trials did not affect hemoglobin, serum total protein, albumin, globulin, glucose, and total cholesterol, except the packed cell volume which was increased in the additive treatments with restriction at the end of the experiment. Moreover, the dietary treatments did not influence the carcass yield. However, the relative weights of liver, gizzard and proventriculus, small intestine and bursa of fabricius were found to be increased. The additives decreased the visible fat in the carcass, with more decreasing effect in the additive groups with restriction. The lowest feed cost per kg of weight gain was observed in the birds fed diets supplemented with synbiotic, probiotic and prebiotic. Feed restriction improved the feed conversion ratio, economic return, but decreased the feed intake, serum total cholesterol and visible fat in comparison with non-restricted groups. Conclusion The biological feed additives could be routinely added to broiler diets, especially when a feed restriction program is followed. Finally, it can be recommended to restrict feed, and add probiotic or synbiotic to increase weight, improve feed conversion rate and reduce feed cost of production.

This study was conducted to evaluate the effect of supplying two levels of Acacia nilotica (A. nilotica) pods to rations of sheep on nutrient digestibility, nitrogen balance and rumen liquor parameters (pH, total protozoa count, protein concentration and enzymes activity). Twelve mature rams (50 ± 1.25 kg B.W.) were distributed into three groups, each with four rams. Animals in group one were considered as a control which fed a basal diet, consisting of concentrate mixture and Egyptian clover. The second group and the third one received the same basal diet with supplying the concentrate mixture by 1.5% and 3.0% of A. nilotica pods meal respectively. The experiment lasted for 3 weeks. It was found that supplementation of A. nilotica pods to the concentrate mixture at a rate of 1.5% and 3.0% significantly improved the total feed intake compared to the control. The digestibility of dry matter and crude fibre was significantly reduced with A. nilotica supplements, whereas the digestibility of crude protein was significantly improved. All of nitrogen intake and N-retained were significantly increased in rams fed on concentrates with 1.5% and 3.0% A. nilotica pods when compared to the control. The pH of ruminal fluid was not affected by the dietary treatments. Nevertheless, the total rumen protozoa count was significantly decreased in A. nilotica pods supplemented groups. Also, the rumen protein concentration and the ruminal enzymes activity, especially α-amylase, cellulase and protease, were lower in A. nilotica pods supplemented treatments. In conclusions, inclusion of low levels of A. nilotica pods (1.5% and 3.0%) in the concentrates can be used as a natural protein protectant in ruminants by forming tannin protein complexes in the rumen to maximize the amino acids available in the lower digestive tract. Also, these levels can increase the protein digestibility as well as the N-retained in the body .

Phytogenic feed additives represent a potential alternative to antibiotics with attributed health and growth-promoting effects. Chickens supplemented with an essential oil blend, a Quillaja saponin blend, or a combination of both phytogenic preparations showed a comprehensively and significantly improved apparent ileal digestibility of crude protein and amino acids compared to control birds. Accordingly, holistic transcriptomic analyses of jejunum and liver samples indicated alterations of macromolecule transporters and processing pathways likely culminating in an increased uptake and metabolizing of carbohydrates and fatty acids. Complementary analyses in Caco-2 showed a significant increase in transporter recruitment to the membrane (SGLT1 and PEPT1) after addition of essential oils and saponins. Although the penetrance of effects differed for the used phytogenic feed additives, the results indicate for an overlapping mode of action including local effects at the intestinal border and systemic alterations of macronutrient metabolism resulting in an improved performance of broilers.

Summary This study was conducted to investigate the effects of including potato peels (PP) and sugar beet pulp (SBP), as unconventional feeds, with and without enzyme in broiler diets from 1 to 42 days of age by observing the growth performance, blood parameters and carcass characteristics. A total of 150, 1-day old, chicks were randomly assigned into five groups, each with 30 chicks. Birds in group 1 were fed on the control diet. Chicks in groups 2 and 3 were offered diets containing PP and SBP at the rate of 15% and 7.5%, respectively, while those in groups 4 and 5 were fed the same diets but with adding an enzyme mixture. Using the unconventional feeds in the diets was found to decrease the body weight (BW). However, the feed intake, weight gain and feed conversion did not differ from the control in PP at the grower period, but decreased in SBP throughout the experiment. Addition of enzyme greatly improved the BW in PP and SBP to a degree that it surpasses the control and also increased the feed intake and conversion. The total cholesterol and low-density lipoprotein cholesterol serum levels were decreased in all tested groups. Carcass yield was not affected by treatments, but the carcass fat content was reduced using the unconventional feeds with or without enzyme. In conclusion, PP can be used at a rate of 15% in the grower diets of broilers. Furthermore, 15% PP or 7.5% SBP can be included in starter and grower diets, but with the addition of enzymes. This can help in solving the problem of current shortage and rising costs of conventional feeds.

Foot pad dermatitis (FPD) is very common in turkeys and it is an animal health and welfare issue affecting not only performance and walking ability but also the carcass quality. Thus, there is a great need to find out the preventive measures against this problem. The potential roles of extra dietary biotin, Zn and mannan-oligosaccharides (MOS) in preventing the development of FPD were assessed in this study which was conducted on 2-week-old female turkeys over a period of 4 weeks. The birds were allotted to four groups, with 29 each, and housed on dry wood shavings in floor pens. The turkeys were fed a control (300 μg biotin and 50 mg Zn/kg), high biotin (2000 μg/kg), high Zn (150 mg/kg) or MOS (1%) diet. Half of the turkeys in each group were additionally exposed to wet litter (27% DM) for 8 h daily in adjacent separate boxes. Foot pads of the birds were examined on days 0, 7, 14, 21 and 28 and assessed macroscopically and histopathologically for foot pad lesions. High dietary levels of biotin or Zn significantly reduced the severity of FPD on dry litter (score 1 vs. 2 in control) but not on wet litter. However, MOS did not affect the severity of foot pad lesions either on dry or wet litter. In addition, the severity was overall substantially higher on wet (approximately three times) than on dry litter. The present results suggest that adding high levels of biotin or Zn to the diet could reduce the development and severity of FPD on dry litter but without having any preventive effects on wet litter. Finally, the high litter moisture appears to be the major factor resulting in FPD. Therefore, the litter should be maintained dry to minimize the prevalence and severity of FPD in turkeys.

Since turkeys are in continuous contact with the litter, the potential effects of bedding materials and their quality are of major concern in the etiology of Foot Pad Dermatitis (FPD). Two week-old female turkeys were allotted to four groups, with 29 in each and housed either on wood shavings, lignocellulose, chopped straw or dried maize silage over a period of four weeks without artificial climatisation. The birds in all groups were fed identical commercial diets. Half of the turkeys in each group were additionally exposed for 8 h/d to corresponding wet (27% DM; by adding water) litter in adjacent separate boxes. Foot pads of the birds were examined macroscopically and histologically at the start and end of the experiment as well as at weekly intervals. Lignocellulose showed the lowest severity of FPD on dry and wet litter treatments, whereas chopped straw was associated with high FPD scores on dry treatment. Foot pad scores were similar on wood shavings and dried maize silage whether dry or wet. The DM content of litter materials was determined and the highest moisture content among dry treatments was observed in straw (about 31%) which was paralleled with FPD severity. The severity of FPD was overall much higher (>2 times) on wet than on dry litter. Exposure of the birds to wet litter for 8 h/d was sufficient to develop FPD. Lignocellulose could reduce the FPD severity, probably due to higher water binding capacity and faster release of water, while straw may increase it due to lower water evaporation. The physical form of litter either soft (lignocellulose) or with sharp edges (chopped straw) may also affect the onset of FPD. The litter moisture appears to be the dominant factor resulting in the development of FPD and should be kept lower than about 30% to minimise the prevalence and severity of FPD in turkeys.

Soybean meal (SBM) is the main protein source in diets for turkeys. High dietary levels of SBM are thought to increase the incidence of foot pad dermatitis (FPD). Therefore, this study was conducted to test potential effects of high SBM and to elucidate which constituents in SBM might be associated with the development of FPD. Two week-old female turkeys were allotted to four groups of 29 birds each, and housed on dry wood shavings in floor pens over a period of three weeks. Four different diets were fed: control, high SBM, high potassium (K) or high oligosaccharide (OL) diet. Additionally, for only 8 h/d half of the animals in each group were exposed to wet litter (27% DM) in adjacent separate boxes. The foot pads of all birds were assessed on days 0, 7, 14 and 21 for external lesions. For the histopathology of the foot pads, on day 0 three birds from each group, and on days 7 and 14 six birds per feeding group were selected. The remaining birds in each group were sacrificed on day 21 and their pads were evaluated histologically. High dietary levels of SBM, potassium or oligosaccharides did not influence the severity of FPD on dry litter, but slightly increased the severity on wet litter. However, there were no histopathological differences in FPD severity between these dietary treatments within each litter form compared to the control. Nevertheless, the FPD severity was in general higher on wet litter. Thus, litter moisture appears to be one of the most important factors involved in FPD in turkeys. In addition, all nutritional factors which increase water intake and excreta or litter moisture may contribute to an increased development and severity of FPD in turkeys.

Male broiler chicks (n=120) were fed diets containing 0, 5, 10 or 15% dried distillers' grains with solubles (DDGS) from the 12th day up to the end of fattening (day 35). During this period feed intake, weight gain and excreta quality (pH, DM) were tested. A digestibility trial was carried out on four birds from each group on the last five days of the experiment to determine the digestibility of organic matter and CP of the different diets. The protein digestibility was evaluated using three different methods; uric acid correction, alpha-amino-N and amino acid-N. There were no significant effects of increased DDGS levels on feed intake, weight gain, excreta quality or digestibility of CP and organic matter. However, feed conversion showed a tendency to decline at the highest DDGS level (15%). Digestibility of DDGS protein was estimated to be 77%. There was no significant difference between uric acid and alpha-amino-N method, but both methods had a significantly lower CP digestibility than amino acid-N. The present results indicate that DDGS can be used as a protein source in diets for fattening broilers up to 10-15%.

High dietary protein is thought to increase the incidence of foot pad dermatitis (FPD) as a result of increased uric acid and secondary ammonia production in the excreta or litter. This study was conducted on female turkeys over a period of 3 wk to test the effects of water alone, and also of these end products of protein metabolism, independent of the presence of excreta, on the development and severity of FPD. The animals were allocated into four groups, with 20 birds in each, and housed in floor pens on dry, clean wood shavings (changed daily) throughout the experiment. The control group was housed continuously in its pen, whereas the other groups were additionally exposed daily (for 8 hr) to experimentally treated wood shavings, in adjacent separate boxes, enriched with water alone or water with NH4Cl or uric acid. NH4Cl and uric acid were added via water to the litter to achieve the concentrations of ammonia and uric acid in the litter as found in the excreta of turkeys (about 0.50 g ammonia and 20 g uric acid/kg). The wet litter was kept clean by removing the excreta twice daily and by changing the litter twice a week. The foot pads of all birds were examined on days 0, 7, 14, and 21 and scored externally (macroscopically). Three birds were selected from each group on days 0, 7, and 14, while the remaining 11 birds/group were sacrificed on day 21 for histopathologic assessment of foot pads. The severity of FPD was found to be markedly higher (about 3 times) on wet than on dry litter. There were no negative effects of ammonia and uric acid on foot pad lesions. The results indicate that high litter moisture is the most likely factor causing FPD in turkeys. A focus on nitrogenous irritants in the litter was not substantiated. Exposure of birds to wet litter (in the absence of excreta) for only 8 hr/day was sufficient to develop foot pad lesions. The present results suggest that a focus on the protein content of the diet as a cause of FPD may be misplaced, but all dietary factors which increase excreta or litter moisture, or both, should be considered. The prevalence and severity of FPD can be reduced by maintaining dry litter.

Objective: This study aimed to investigate the effect of dietary copper either deficiency or excess on the plasma lipid profile, glutathione (GSH), and homocysteine levels. Design: Three groups of male albino rats were served as control (fed diet containing the required level of Cu; 5.40 mg/kg), copper-deficient (fed diet containing Cu; 0.60 mg/kg) and copper-excess (fed diet containing high level of Cu; 15.0 mg/kg). Materials and Methods: At the end of the experimental period (6 weeks), plasma levels of copper, lipid profile, GSH and homocysteine were estimated in all tested rats groups. Results: There was a significant increase in triacylgycerols, total cholesterol (T.Ch.), high density lipoprotein-cholesterol (HDL-C), very low density lipoprotein- cholesterol, and low density lipoprotein-cholesterol in rats fed a copperdeficient diet compared to control and those fed copper-excess one. In rats fed copper-deficient, HDL-C/T. Ch. ratio was significantly lower than that of the other tested animals. In addition, plasma GSH increased significantly, while plasma homocysteine decreased significantly in copper-deficient group compared to control and copper-excess groups. A significant negative correlation between plasma copper and each of T.Ch. and plasma GSH was observed in control, copper-deficient and copper-excess groups. On the contrary, plasma copper has shown a significant positive correlation with plasma homocysteine in all groups. Conclusion: the present results indicate that feeding of diets rich in bread and powdered milk develop copper-deficiency that resulted in an increase in plasma T.Ch. and triacylglycerols, while decreased HDL-C/T.Ch. ratio. This is representing a risk factor for the development of cardiovascular disorders. However, the body is able to protect itself against these risky changes by increasing the production of antioxidant (GSH) on the expense of homocysteine. Furthermore, copper supplementation can alleviate these degenerative changes.