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Your search keyword '"Iavicoli, Patrizia"' showing total 39 results
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39 results on '"Iavicoli, Patrizia"'

6. Tuning the supramolecular chirality of one- and two-dimensional aggregates with the number of stereogenic centers in the component porphyrins

Author

Iavicoli, Patrizia, Hong Xu, Feldborg, Lise N., Linares, Mathieu, Paradinas, Markos, Stafstrom, Sven, Ocal, Carmen, Nieto-Ortega, Belen, Casado, Juan, Lopez Navarrete, Juan T., Lazzaroni, Roberto, Feyter, Steven De, and Amabilino, David B.

Subjects
Suprachiasmatic nucleus -- Analysis, Chirality -- Analysis, Porphyrins -- Chemical properties, Porphyrins -- Electric properties, Chemical synthesis -- Analysis, Chemistry
Abstract

A synthetic strategy was developed to perform synthesis of porphyrins containing between one and four stereogenic centers, such that their molecular weights vary only as a result of methyl groups which give the chiral forms. The self-assembly and chiral structure of the aggregates of porphyrin molecules are observed to be significantly influenced by the number of stereogenic centers.

Published
2010

9. Quantification of interacting cognate odorants with olfactory receptors in nanovesicles

Author

European Research Council, Marco, M. Pilar [0000-0002-4064-1668], Sanmartí-Espinal, Marta, Iavicoli, Patrizia, Calò, Annalisa, Taulés, Marta, Galvé, Roger, Marco, María Pilar, Samitier, Josep, European Research Council, Marco, M. Pilar [0000-0002-4064-1668], Sanmartí-Espinal, Marta, Iavicoli, Patrizia, Calò, Annalisa, Taulés, Marta, Galvé, Roger, Marco, María Pilar, and Samitier, Josep

Abstract

This study aims to improve our understanding of the interaction between olfactory receptors and odorants to develop highly selective biosensing devices. Natural nanovesicles (NVs) from Saccharomyces cerevisiae, ∼100 nm in diameter, carrying either the human OR17-40 or the chimpanzee OR7D4 olfactory receptor (OR) tagged with the c-myc epitope at their N-terminus, are presented as model systems to quantify the interaction between odorant and olfactory receptors. The level of expression of olfactory receptors was determined at individual NVs using a novel competitive ELISA immunoassay comparing the values obtained against those from techniques involving the solubilization of cell membrane proteins and the identification of c-myc-carrying receptors. Surface Plasmon Resonance (SPR) measurements on L1 Biacore chips indicate that cognate odorants bind to their Ors, thereby quantifying the approximate number of odorants that interact with a given olfactory receptor. The selectivity of OR17-40-carrying NVs towards helional and OR7D4-carrying NVs towards androstenone has been proven in cross-check experiments with non-specific odorant molecules (heptanal and pentadecalactone, respectively) and in control receptors. © 2017 The Author(s).

Published
2017

10. Engineering monolayer poration for rapid exfoliation of microbial membranes

Author

Pyne, Alice, primary, Pfeil, Marc-Philipp, additional, Bennett, Isabel, additional, Ravi, Jascindra, additional, Iavicoli, Patrizia, additional, Lamarre, Baptiste, additional, Roethke, Anita, additional, Ray, Santanu, additional, Jiang, Haibo, additional, Bella, Angelo, additional, Reisinger, Bernd, additional, Yin, Daniel, additional, Little, Benjamin, additional, Muñoz-García, Juan C., additional, Cerasoli, Eleonora, additional, Judge, Peter J., additional, Faruqui, Nilofar, additional, Calzolai, Luigi, additional, Henrion, Andre, additional, Martyna, Glenn J., additional, Grovenor, Chris R. M., additional, Crain, Jason, additional, Hoogenboom, Bart W., additional, Watts, Anthony, additional, and Ryadnov, Maxim G., additional

Published
2017
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13. Accurate c-myc-olfactory receptor quantification on characterized natural nanovesicles for biosensor applications

Author

Sanmarti-Espinal, Marta, Iavicoli, Patrizia, Galve, R, Persuy, Marie-Annick, Pajot-Augy, Edith, Marco, M.P., Samitier, J., Institute for Bioengineering of Catalonia [Barcelona] (IBEC), Department of Electronics, Universitat de Barcelona (UB), Consejo Superior de Investigaciones Científicas [Madrid] (CSIC), Neurobiologie de l'Olfaction et Modélisation en Imagerie (NOeMI), Institut National de la Recherche Agronomique (INRA), and Centro de Investigación Biomédica en Red, en Bioingeniera, Biomateriales y Nanomedicina (CIBER-BBN)

Subjects
olfactory receptor, G-protein coupled receptor, membrane receptor, [SDV.NEU]Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC], biosensor, nanovesicle
Abstract

International audience; Natural vesicles produced from genetically engineered cells with tailored membrane receptor composition are promising building blocks for biosensing devices [1]. This is particularly true for the case of G - protein coupled receptors (GPC Rs) present in many sensing processes in cells, whose functionality crucially depends on their lipid environment. However, the controlled production of natural vesicles containing GPCRs and their GPCRs expression level are among the outstanding challenges in the road map to realize practical biomolecular devices based on GPCRs. Largely expressed in the human body, GPCRs are implicated in a wide range of disease pathways [2], including cancer [3,4], cardiomyopathy, and schizophrenia. Around 50% of the drugs on the market regulate GPCR function, 30% of which directly target GPCRs [5]. Herein we present the production and characterization of membrane nanovesicles (nanosomes) of about 100 nm diameter from Saccaromyces Cerevisiae carrying the heterologously expre ssed olfactory receptor c - myc - OR1740 [6], a member of the family of GPCRs and representative of important candidates for the development of bioelectronic noses [7]. The nanosomes size in solution was characterized by Dynamic Light Scattering (DLS), Nanopar ticle Tracking Analysis (NTA), and Cryo - Electron Microscopy. In addition, we describe a universal direct novel c - myc - GPCR quantification without the necessity of a previous protein purification step. The quantification was carried out by a highly promising strategy based on a competitive ELISA format assay using c - myc tag - bioconjugates as model conjugates that mimics the behavior of the c - myc olfactory receptor on nanovesicles. A custom made antibody (anti - c - myc) was produced giving assay detectability in t he picomolar range and allowing the quantification of the c - myc - OR proteins. The number of olfactory receptors (OR17 - 40) per nanovesicle obtained was (30 +/- 6) OR/NS. Evidences of the OR17 - 40 functionality have been obtained by Biacore (SPR) measurements. Present results constitute an important step in the practical realization of biosensor devices based on natural nanovesicles integrating G - protein coupled membrane receptors.

Published
2012

14. Bioelectronic odorant detection nanoplatform

Author

Pajot, Edith, Daligault, Julien, Badonnel, Karine, Dewaele, Aurélie, Persuy, Marie-Annick, Congar, Patrice, Meunier, Nicolas, Baly, Christine, Salesse, Roland, Zine, Nadia, Baraket, Abdellatif, Errachid, Abdelhamid, Calo, Annalisa, Iavicoli, Patrizia, Sanmarti, Marta, Jaffrezic-Renault, Nicole, Gomila, Gabriel, Samitier, Josep, Neurobiologie de l'Olfaction et Modélisation en Imagerie (NOeMI), Institut National de la Recherche Agronomique (INRA), Institut des Sciences Analytiques (ISA), Institut de Chimie du CNRS (INC)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS), Université Claude Bernard Lyon 1 (UCBL), Université de Lyon, lab. Nanobioenginyeria, Institut de Bioenginyeria de Catalunya (IBEC), and Institute for Bioengineering of Catalonia [Barcelona] (IBEC)

Subjects
Biosensors, Odorant, [SDV]Life Sciences [q-bio], Nanotechnology, [INFO]Computer Science [cs], Olfactory receptors, ComputingMilieux_MISCELLANEOUS, Electronic nose
Abstract

International audience

Published
2012

15. Biosensor based on olfactory receptors immobilization for the detection of odorant compounds

Author

Sanmarti, Marta, Iavicoli, Patrizia, Pajot-Augy, Edith, Dols-Perez, Aurora, Calo, Annalisa, Gomila, Gabriel, Samitier, Josep, Institute for Bioengineering of Catalonia [Barcelona] (IBEC), Neurobiologie de l'Olfaction et Modélisation en Imagerie (NOeMI), Institut National de la Recherche Agronomique (INRA), International Society for Olfaction and Chemical Sensing (ISOCS). INT., and ProdInra, Migration

Subjects
artificial nose, olfactory receptor, odor, nanosome, microscopy, food and beverages, [SDV.NEU]Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC], [SDV.NEU] Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC]
Abstract

International audience; The olfactory system can discriminate quickly thousands of odorants at very low concentrations. With the aim of building a sensor that can detect odorants with high specificity and sensitivity, a new generation of artificial noses using olfactory receptors is being developed in our group. The possibility of using olfactory receptors for sensing in electronic noses was demonstrated in a previous work. Following those obtained results, we designed a new artificial olfactory system with the goal of developing a robust and reproducible odor-sensing device. This bioelectronic nose will be capable of detecting specific odorants which can be used in the area of food quality control.

Published
2011

16. Biosensor based on olfactory receptors immobilization for the detection of odorant compounds

Author

Iavicoli, Patrizia, Sanmarti, Marta, Pajot, Edith, Gomila, Gabriel, Samitier, Josep, Institute for Bioengineering of Catalonia [Barcelona] (IBEC), Neurobiologie de l'Olfaction et Modélisation en Imagerie (NOeMI), Institut National de la Recherche Agronomique (INRA), and ProdInra, Migration

Subjects
[SDV] Life Sciences [q-bio], [SDV]Life Sciences [q-bio], [INFO]Computer Science [cs], [INFO] Computer Science [cs], ComputingMilieux_MISCELLANEOUS
Abstract

International audience

Published
2011

17. Nanosomes immobilization for a bioelectronic olfactory neuron device

Author

Sanmarti, Marta, Iavicoli, Patrizia, Pajot, Edith, Samitier, Josep, Institute for Bioengineering of Catalonia [Barcelona] (IBEC), Neurobiologie de l'Olfaction et Modélisation en Imagerie (NOeMI), Institut National de la Recherche Agronomique (INRA), and ProdInra, Migration

Subjects
[SDV] Life Sciences [q-bio], [SDV]Life Sciences [q-bio], [INFO]Computer Science [cs], [INFO] Computer Science [cs], ComputingMilieux_MISCELLANEOUS
Abstract

International audience

Published
2011

18. Photoconductivity of Nanofilaments That are Self-Assembled from a Porphyrin with Long Alkyl-Chain Substituents

Author

Schall, Anna P., Iavicoli, Patrizia, John Qi, Zhengling, Menko, Julien, Lu, Ye, Linares, Mathieu, de Paula, Julio C., Amabilino, David B., Johnson, A. T., Smith, Walter F., Schall, Anna P., Iavicoli, Patrizia, John Qi, Zhengling, Menko, Julien, Lu, Ye, Linares, Mathieu, de Paula, Julio C., Amabilino, David B., Johnson, A. T., and Smith, Walter F.

Abstract

Photoelectronically active nanostructures that are Self-assembled from organic rnolecules hold the prortu se of tailored functionality.with simple and inexpensive production. Comparison of nanoWires assembled from related componnds can give important insightS into the details of self-assembly and the,conduction meehanisms. We report the photoconductivity of nanofibers that are self-assembled from a porphyrin With long alkyl substituents. In contrast to previously studied porphyrin nanowires, the photoconductivity increases as atthospheric O-2 is increased. This can be explained-using the same model aS used in the previous studies, by assuming a different, line-up of the bands of the nanofilaments. with the electrode Fermi level. However, this model does not explain our observation that-at O-2 concentrations above 1%, the conduction increases with continued- illunation; this may be due to photoactivation of shallow O-2 adsorption sites. The overall conduction level is low even at high O-2 Concentration, because the alkyl substituents form an insulating sheath around the rianofibers. Such inSulation could be valuable in applications where it would prevent cross-talk between signal S darried in different nanofilaments. Schottky barriers at the interface between- organic nanostructures and electrodes strongly affect conduction and photoconduction, and are strongly influenced by atmospheric gases such, as O-2., Funding Agencies|National Science Foundation [CHE-0616615, BMAT-1306170]; Nano/Bio Interface Center at the University of Pennsylvania through the National Science Foundation [NSEC DMR08-32802]; MINECO (Spain) [CTQ2010-16339, MAT2013-47869-C4]; IBM Ph.D. Fellowship; NSF IGERT program [DGE02-21664]; SeRC (Swedish e-Science Research Center)

Published
2015
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23. Pasteurian Segregation on a Surface Imaged In Situ at the Molecular Level

Author

Xu, Hong, primary, Saletra, Wojciech J., additional, Iavicoli, Patrizia, additional, Van Averbeke, Bernard, additional, Ghijsens, Elke, additional, Mali, Kunal S., additional, Schenning, Albertus P. H. J., additional, Beljonne, David, additional, Lazzaroni, Roberto, additional, Amabilino, David B., additional, and De Feyter, Steven, additional

Published
2012
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25. Organization of the enantiomeric and racemic forms of an amphiphilic resorcinol derivative at the air-water and graphite-1-phenyloctane interfaces

Author

Iavicoli, Patrizia, primary, XU, Hong, additional, Keszthelyi, TamáS, additional, Telegdi, Judit, additional, WURST, Klaus, additional, Van Averbeke, Bernard, additional, Saletra, Wojciech J., additional, Minoia, Andrea, additional, Beljonne, David, additional, Lazzaroni, Roberto, additional, De Feyter, Steven, additional, and Amabilino, David B., additional

Published
2011
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32. Monolayer self-assembly at liquid–solid interfaces: chirality and electronic properties of molecules at surfaces

Author

Amabilino, David B, primary, De Feyter, Steven, additional, Lazzaroni, Roberto, additional, Gomar-Nadal, Elba, additional, Veciana, Jaume, additional, Rovira, Concepció, additional, Abdel-Mottaleb, Mohamed M, additional, Mamdouh, Wael, additional, Iavicoli, Patrizia, additional, Psychogyiopoulou, Krystallia, additional, Linares, Mathieu, additional, Minoia, Andrea, additional, Xu, Hong, additional, and Puigmartí-Luis, Josep, additional

Published
2008
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38. Chiral expression at the solid-liquid interface: a joint experimental and theoretical study of the self-assembly of chiral porphyrins on graphite.

Author

Linares M, Iavicoli P, Psychogyiopoulou K, Beljonne D, De Feyter S, Amabilino DB, and Lazzaroni R

Subjects
Adsorption, Chemistry methods, Computer Simulation, Hydrogen Bonding, Microscopy, Scanning Tunneling methods, Models, Chemical, Models, Molecular, Models, Theoretical, Molecular Conformation, Software, Solvents chemistry, Stereoisomerism, Surface Properties, Graphite chemistry, Porphyrins chemistry
Abstract

The chiral organization of an enantiopure functional molecule on an achiral surface has been studied with the aim of understanding the influence of stereogenic centers on the self-assembly in two dimensions. A chiral tetra meso-amidophenyl-substituted porphyrin containing long hydrophobic tails at the periphery of the conjugated pi-electron system was prepared for this purpose. Scanning tunneling microscopy (STM) images of the compound at the graphite-heptanol interface reveal a chiral arrangement of the molecules, with the porphyrin rows tilted by 13 degrees with respect to the normal to the graphite axes. In terms of molecular modeling, a combination of molecular dynamics simulations on systems constrained by periodic boundary conditions and on unconstrained large molecular aggregates has been applied to reach a quantitative interpretation on both the density of the layer and its orientation with respect to the graphite surface. The results show clearly that (i) the methyl groups of the stereogenic point toward the graphite surface and (ii) the porphyrin molecules self-assemble into an interdigitated structure where the alkyl chains align along one of the graphite axes and the porphyrin cores are slightly shifted with respect to one another. The direction of this shift, which defines the chirality of the monolayer, is set by the chirality of the stereogenic centers. Such an arrangement results in the formation of a dense chiral monolayer that is further stabilized by hydrogen bonding with protic solvents.

Published
2008
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39. Unique intermolecular reaction of simple porphyrins at a metal surface gives covalent nanostructures.

Author

In't Veld M, Iavicoli P, Haq S, Amabilino DB, and Raval R

Subjects
Molecular Structure, Particle Size, Surface Properties, Copper chemistry, Nanostructures chemistry, Organometallic Compounds chemistry, Porphyrins chemistry
Abstract

Deposition of a porphyrin onto metallic copper followed by heating leads to an unprecedented type of linking of the molecules giving a mixture of covalent multiporphyrin nanostructures at the surface.

Published
2008
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