2,806 results on '"Ianiro A"'
Search Results
2. Machine-learned flow estimation with sparse data -- exemplified for the rooftop of a UAV vertiport
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Hou, Chang, Marra, Luigi, Maceda, Guy Y. Cornejo, Jiang, Peng, Chen, Jingguo, Liu, Yutong, Hu, Gang, Chen, Jialong, Ianiro, Andrea, Discetti, Stefano, Meilán-Vila, Andrea, and Noack, Bernd R.
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Physics - Fluid Dynamics - Abstract
We propose a physics-informed data-driven framework for urban wind estimation. This framework validates and incorporates the Reynolds number independence for turbulent flows, thus allowing the extrapolation for wind conditions far beyond the training data. Another key enabler is a machine-learned non-dimensionalized manifold from snapshot data. The velocity field is modeled using a double encoder-decoder approach. The first encoder normalizes data using the oncoming wind speed, while the second encoder projects this normalized data onto the isometric feature mapping manifold. The decoders reverse this process, with $k$-nearest neighbor performing the first decoding and the second undoing the normalization. The manifold is coarse-grained by clustering to reduce the computational load for de- and encoding. The sensor-based flow estimation is based on the estimate of the oncoming wind speed and a mapping from sensor signal to the manifold latent variables. The proposed machine-learned flow estimation framework is exemplified for the flow above an Unmanned Aerial Vehicle (UAV) vertiport. The wind estimation is shown to generalize well for rare wind conditions, not included in the original database.
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- 2024
3. Some effects of limited wall-sensor availability on flow estimation with 3D-GANs
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Cuéllar, Antonio, Ianiro, Andrea, and Discetti, Stefano
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Physics - Fluid Dynamics - Abstract
In this work we assess the impact of the limited availability of wall-embedded sensors on the full 3D estimation of the flow field in a turbulent channel with Re{\tau} = 200. The estimation technique is based on a 3D generative adversarial network (3D-GAN). We recently demonstrated that 3D-GANs are capable of estimating fields with good accuracy by employing fully-resolved wall quantities (pressure and streamwise/spanwise wall shear stress on a grid with DNS resolution). However, the practical implementation in an experimental setting is challenging due to the large number of sensors required. In this work, we aim to estimate the flow fields with substantially fewer sensors. The impact of the reduction of the number of sensors on the quality of the flow reconstruction is assessed in terms of accuracy degradation and spectral length-scales involved. It is found that the accuracy degradation is mainly due to the spatial undersampling of scales, rather than the reduction of the number of sensors per se. We explore the performance of the estimator in case only one wall quantity is available. When a large number of sensors is available, pressure measurements provide more accurate flow field estimations. Conversely, the elongated patterns of the streamwise wall shear stress make this quantity the most suitable when only few sensors are available. As a further step towards a real application, the effect of sensor noise is also quantified. It is shown that configurations with fewer sensors are less sensitive to measurement noise., Comment: in Theoretical and Computational Fluid Dynamics (2024)
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- 2024
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4. Three-dimensional generative adversarial networks for turbulent flow estimation from wall measurements
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Cuéllar, Antonio, Güemes, Alejandro, Ianiro, Andrea, Flores, Óscar, Vinuesa, Ricardo, and Discetti, Stefano
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Physics - Fluid Dynamics - Abstract
Different types of neural networks have been used to solve the flow sensing problem in turbulent flows, namely to estimate velocity in wall-parallel planes from wall measurements. Generative adversarial networks (GANs) are among the most promising methodologies, due to their more accurate estimations and better perceptual quality. This work tackles this flow sensing problem in the vicinity of the wall, addressing for the first time the reconstruction of the entire three-dimensional (3-D) field with a single network, i.e. a 3-D GAN. With this methodology, a single training and prediction process overcomes the limitation presented by the former approaches based on the independent estimation of wall-parallel planes. The network is capable of estimating the 3-D flow field with a level of error at each wall-normal distance comparable to that reported from wall-parallel plane estimations and at a lower training cost in terms of computational resources. The direct full 3-D reconstruction also unveils a direct interpretation in terms of coherent structures. It is shown that the accuracy of the network depends directly on the wall footprint of each individual turbulent structure. It is observed that wall-attached structures are predicted more accurately than wall-detached ones, especially at larger distances from the wall. Among wall-attached structures, smaller sweeps are reconstructed better than small ejections, while large ejections are reconstructed better than large sweeps as a consequence of their more intense footprint.
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- 2024
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5. A meshless method to compute the proper orthogonal decomposition and its variants from scattered data
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Tirelli, Iacopo, Mendez, Miguel Alfonso, Ianiro, Andrea, and Discetti, Stefano
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Physics - Data Analysis, Statistics and Probability - Abstract
Complex phenomena can be better understood when broken down into a limited number of simpler "components". Linear statistical methods such as the principal component analysis and its variants are widely used across various fields of applied science to identify and rank these components based on the variance they represent in the data. These methods can be seen as factorizations of the matrix collecting all the data, which are assumed to be a collection of time series sampled from fixed points in space. However, when data sampling locations vary over time, as with mobile monitoring stations in meteorology and oceanography or with particle tracking velocimetry in experimental fluid dynamics, advanced interpolation techniques are required to project the data onto a fixed grid before carrying out the factorization. This interpolation is often expensive and inaccurate. This work proposes a method to decompose scattered data without interpolating. The approach is based on physics-constrained radial basis function regression to compute inner products in space and time. The method provides an analytical and mesh-independent decomposition in space and time, demonstrating higher accuracy than the traditional approach. Our results show that it is possible to distill the most relevant "components" even for measurements whose natural output is a distribution of data scattered in space and time, maintaining high accuracy and mesh independence.
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- 2024
6. Actuation manifold from snapshot data
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Marra, Luigi, Maceda, Guy Y. Cornejo, Meilán-Vila, Andrea, Guerrero, Vanesa, Rashwan, Salma, Noack, Bernd R., Discetti, Stefano, and Ianiro, Andrea
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Physics - Fluid Dynamics ,Mathematics - Dynamical Systems ,Mathematics - Optimization and Control ,Physics - Data Analysis, Statistics and Probability - Abstract
We propose a data-driven methodology to learn a low-dimensional actuation manifold of controlled flows. The starting point is resolving snapshot flow data for a representative ensemble of actuations. Key enablers for the actuation manifold are isometric mapping as encoder and k-nearest neighbour regression as a decoder. This methodology is tested for the fluidic pinball, a cluster of three parallel cylinders perpendicular to the oncoming uniform flow. The centers of these cylinders are the vertices of an equilateral triangle pointing upstream. The flow is manipulated by constant rotation of the cylinders, i.e. described by three actuation parameters. The Reynolds number based on a cylinder diameter is chosen to be 30. The unforced flow yields statistically symmetric unforced periodic shedding represented by a one-dimensional limit cycle. The proposed methodology yields a five-dimensional manifold describing a wide range of dynamics with small representation error. Interestingly, the manifold coordinates automatically unveil physically meaningful parameters. Two of them describe the downstream periodic vortex shedding. The other three ones describe the near-field actuation, i.e. the strength of boat-tailing, the Magnus effect and forward stagnation point. The manifold is shown to be a key enabler for control-oriented flow estimation., Comment: 10 pages, 5 figures
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- 2024
7. Adipokines: masterminds of metabolic inflammation
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Tilg, Herbert, Ianiro, Gianluca, Gasbarrini, Antonio, and Adolph, Timon E.
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- 2024
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8. The importance of gut microbiome in the perinatal period
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Catassi, Giulia, Mateo, Sandra Garcia, Occhionero, Annamaria Sara, Esposito, Chiara, Giorgio, Valentina, Aloi, Marina, Gasbarrini, Antonio, Cammarota, Giovanni, and Ianiro, Gianluca
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- 2024
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9. Large-area, self-healing block copolymer membranes for energy conversion
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Sproncken, Christian C. M., Liu, Peng, Monney, Justin, Fall, William S., Pierucci, Carolina, Scholten, Philip B. V., Van Bueren, Brian, Penedo, Marcos, Fantner, Georg Ernest, Wensink, Henricus H., Steiner, Ullrich, Weder, Christoph, Bruns, Nico, Mayer, Michael, and Ianiro, Alessandro
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- 2024
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10. Innovation ecosystems: a comparison between university spin-off firms and innovative start-ups. Evidence from Italy
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Modina, Michele, Capalbo, Francesco, Sorrentino, Marco, Ianiro, Gabriele, and Khan, Muhammad Fayaz
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- 2024
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11. A Customized 3D-Printed Bolus for High-Risk Breast Cancer with Skin Infiltration: A Pilot Study
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Silvia Takanen, Anna Ianiro, Paola Pinnarò, Erminia Infusino, Laura Marucci, Antonella Soriani, Giuseppe Sanguineti, and Giuseppe Iaccarino
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breast cancer ,skin toxicity ,dosimetric analysis ,3D-printed bolus ,Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,RC254-282 - Abstract
Background: In high-risk breast cancer patients with skin infiltration, the administration of a uniform dose to superficial tissues is fundamental in order to reduce local skin relapse. A personalized bolus may prevent the potential inadequate dose distribution of a standard bolus due to air gaps between the bolus and the skin. In this pilot study, we introduced into clinical practice the use of a personalized 3D-printed bolus filled with ultrasound transmission gel. Methods: Seven patients undergoing radiotherapy after mastectomy were selected. A 3D-printed bolus dosimetric assessment was performed with MOSFET dosimeters on an anthropomorphic phantom and, subsequently, on three selected cases with increasing bolus shape irregularity. Acute/late toxicity and local control were assessed. Results: Overall, for the clinical cases, the percentage median difference between the measured and calculated doses was −2.7% (−7.0–4.9%). The median follow-up was 21 months. After two years, one patient showed G2 pain, one patient manifested G1 telangiectasia, one patient showed G1 hyperpigmentation, and two patients had no relevant toxicity. Conclusions: A personalized 3D-printed bolus filled with ultrasound gel may easily reproduce the standard bolus’ consistency and provide accurate coverage of the target area with tolerable acute/late toxicity grades. This is a pilot study, and further investigations are needed.
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- 2024
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12. A greener gastroenterology: challenges and opportunities for an eco-sustainable approach to digestive diseases
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Cammarota, Giovanni and Ianiro, Gianluca
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- 2024
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13. Identification of spatially-resolved markers of malignant transformation in Intraductal Papillary Mucinous Neoplasms
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Agostini, Antonio, Piro, Geny, Inzani, Frediano, Quero, Giuseppe, Esposito, Annachiara, Caggiano, Alessia, Priori, Lorenzo, Larghi, Alberto, Alfieri, Sergio, Casolino, Raffaella, Scaglione, Giulia, Tondolo, Vincenzo, Cammarota, Giovanni, Ianiro, Gianluca, Corbo, Vincenzo, Biankin, Andrew V., Tortora, Giampaolo, and Carbone, Carmine
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- 2024
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14. Can the Evidence-Based Use of Probiotics (Notably Saccharomyces boulardii CNCM I-745 and Lactobacillus rhamnosus GG) Mitigate the Clinical Effects of Antibiotic-Associated Dysbiosis?
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Waitzberg, Dan, Guarner, Francisco, Hojsak, Iva, Ianiro, Gianluca, Polk, D. Brent, and Sokol, Harry
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- 2024
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15. Genetically-inspired convective heat transfer enhancement in a turbulent boundary layer
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Castellanos, Rodrigo, Ianiro, Andrea, and Discetti, Stefano
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Physics - Fluid Dynamics ,Statistics - Machine Learning - Abstract
The convective heat transfer in a turbulent boundary layer (TBL) on a flat plate is enhanced using an artificial intelligence approach based on linear genetic algorithms control (LGAC). The actuator is a set of six slot jets in crossflow aligned with the freestream. An open-loop optimal periodic forcing is defined by the carrier frequency, the duty cycle and the phase difference between actuators as control parameters. The control laws are optimised with respect to the unperturbed TBL and to the actuation with a steady jet. The cost function includes the wall convective heat transfer rate and the cost of the actuation. The performance of the controller is assessed by infrared thermography and characterised also with particle image velocimetry measurements. The optimal controller yields a slightly asymmetric flow field. The LGAC algorithm converges to the same frequency and duty cycle for all the actuators. It is noted that such frequency is strikingly equal to the inverse of the characteristic travel time of large-scale turbulent structures advected within the near-wall region. The phase difference between multiple jet actuation has shown to be very relevant and the main driver of flow asymmetry. The results pinpoint the potential of machine learning control in unravelling unexplored controllers within the actuation space. Our study furthermore demonstrates the viability of employing sophisticated measurement techniques together with advanced algorithms in an experimental investigation., Comment: 20 pages, 13 figures
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- 2023
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16. A simple trick to improve the accuracy of PIV/PTV data
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Tirelli, Iacopo, Ianiro, Andrea, and Discetti, Stefano
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Physics - Fluid Dynamics - Abstract
Particle Image Velocimetry (PIV) estimates velocities through correlations of particle images within interrogation windows, leading to a spatial modulation of the velocity field. Although in principle Particle Tracking Velocimetry (PTV) estimates locally a non-modulated particle displacement, to exploit the scattered data from PTV it is necessary to interpolate these data on a structured grid, which implies a spatial modulation effect that biases the resulting velocity field. This systematic error due to finite spatial resolution inevitably depends on the interrogation window size and on the interparticle spacing. It must be observed that all these operations (cross-correlation, direct interpolation or averaging in windows) induce modulation on both the mean and the fluctuating part. We introduce a simple trick to reduce this systematic error source of PIV/PTV measurements exploiting ensemble statistics. Ensemble Particle Tracking Velocimetry (EPTV) can be leveraged to obtain the high-resolution mean flow by merging the different instantaneous realisations. The mean flow can be estimated with EPTV, and the fluctuating part can be measured from PIV/PTV. The high-resolution mean can then be superposed to the instantaneous fluctuating part to obtain velocity fields with lower systematic error. The methodology is validated against datasets with a progressively increasing level of complexity: two virtual experiments based on direct numerical simulations (DNS) of the wake of a fluidic pinball and a channel flow and the experimental data of a turbulent boundary layer. For all the cases both PTV and PIV are analysed.
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- 2023
17. Fecal Microbiota Transplantation as Emerging Treatment in European Countries 2.0
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Porcari, Serena, Maida, Marcello, Bibbò, Stefano, McIlroy, James, Ianiro, Gianluca, Cammarota, Giovanni, Mastrantonio, Paola, editor, and Rupnik, Maja, editor
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- 2024
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18. Henoch–schonlein purpura following exposure to SARS-CoV2 vaccine or infection: a systematic review and a case report
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Di Vincenzo, Federica, Ennas, Sara, Pizzoferrato, Marco, Bibbò, Stefano, Porcari, Serena, Ianiro, Gianluca, and Cammarota, Giovanni
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- 2024
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19. Identification of spatially-resolved markers of malignant transformation in Intraductal Papillary Mucinous Neoplasms
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Antonio Agostini, Geny Piro, Frediano Inzani, Giuseppe Quero, Annachiara Esposito, Alessia Caggiano, Lorenzo Priori, Alberto Larghi, Sergio Alfieri, Raffaella Casolino, Giulia Scaglione, Vincenzo Tondolo, Giovanni Cammarota, Gianluca Ianiro, Vincenzo Corbo, Andrew V. Biankin, Giampaolo Tortora, and Carmine Carbone
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Science - Abstract
Abstract The existing Intraductal Papillary Mucinous Neoplasm (IPMN) risk stratification relies on clinical and histological factors, resulting in inaccuracies and leading to suboptimal treatment. This is due to the lack of appropriate molecular markers that can guide patients toward the best therapeutic options. Here, we assess and confirm subtype-specific markers for IPMN across two independent cohorts of patients using two Spatial Transcriptomics (ST) technologies. Specifically, we identify HOXB3 and ZNF117 as markers for Low-Grade Dysplasia, SPDEF and gastric neck cell markers in borderline cases, and NKX6-2 and gastric isthmus cell markers in High-Grade-Dysplasia Gastric IPMN, highlighting the role of TNFα and MYC activation in IPMN progression and the role of NKX6-2 in the specific Gastric IPMN progression. In conclusion, our work provides a step forward in understanding the gene expression landscapes of IPMN and the critical transcriptional networks related to PDAC progression.
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- 2024
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20. Non-intrusive sensing in turbulent boundary layers via deep fully-convolutional neural networks
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Guastoni, L., Balasubramanian, A. G., Güemes, A., Ianiro, A., Discetti, S., Schlatter, P., Azizpour, H., and Vinuesa, R.
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Physics - Fluid Dynamics - Abstract
Fully-convolutional neural networks (FCN) were proven to be effective for predicting the instantaneous state of a fully-developed turbulent flow at different wall-normal locations using quantities measured at the wall. In Guastoni et al. [J. Fluid Mech. 928, A27 (2021)], we focused on wall-shear-stress distributions as input, which are difficult to measure in experiments. In order to overcome this limitation, we introduce a model that can take as input the heat-flux field at the wall from a passive scalar. Four different Prandtl numbers $Pr = \nu/\alpha = (1,2,4,6)$ are considered (where $\nu$ is the kinematic viscosity and $\alpha$ is the thermal diffusivity of the scalar quantity). A turbulent boundary layer is simulated since accurate heat-flux measurements can be performed in experimental settings, paving the way for the implementation of a non-intrusive sensing approach for the flow in practical applications. This is particularly important for closed-loop flow control, which requires an accurate representation of the state of the flow to compute the actuation., Comment: 13 pages, 9 figures
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- 2022
21. List of contributors
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Aimo, Cristina, primary, Aronico, Nicola, additional, Auricchio, Renata, additional, Barone, Michele, additional, Biagi, Federico, additional, Bianchi, Paola Ilaria, additional, Bibbò, Stefano, additional, Broglio, Giacomo, additional, Caio, Giacomo, additional, Calabrese, Emma, additional, Cammarota, Giovanni, additional, Caproni, Marzia, additional, Carroccio, Antonio, additional, Castellaneta, Stefania, additional, Catassi, Carlo, additional, Catassi, Giulia, additional, Ciacci, Carolina, additional, Ciccocioppo, Rachele, additional, Copparoni, Roberto, additional, Corazza, Gino Roberto, additional, Corrà, Alberto, additional, Cristofori, Fernanda, additional, Dargenio, Vanessa Nadia, additional, De Giorgio, Roberto, additional, De Lisi, Giuseppe, additional, De Micco, Ida, additional, Di Sabatino, Antonio, additional, Di Stasio, Luigia, additional, Di Stefano, Michele, additional, Discepolo, Valentina, additional, Fasano, Alessio, additional, Francavilla, Ruggiero, additional, Gasbarrini, Antonio, additional, Gasbarrini, Giovanni, additional, Gatti, Simona, additional, Gianfrani, Carmen, additional, Giuliano, Alessandra, additional, Greco, Luigi, additional, Guandalini, Stefano, additional, Ianiro, Gianluca, additional, Lenti, Marco Vincenzo, additional, Lionetti, Elena, additional, Lucioni, Marco, additional, Maimaris, Stiliano, additional, Mamone, Gianfranco, additional, Mandile, Roberta, additional, Mansueto, Pasquale, additional, Marafini, Irene, additional, Mariotti, Elena Biancamaria, additional, Mengoli, Caterina, additional, Monteleone, Giovanni, additional, Monteleone, Ivan, additional, Neuhold, Susanna, additional, Novellino, Luisa, additional, Pilo, Caterina, additional, Plutino, Giuseppe, additional, Sansotta, Naire, additional, Santacroce, Giovanni, additional, Schiepatti, Annalisa, additional, Seidita, Aurelio, additional, Silano, Marco, additional, Troncone, Edoardo, additional, Troncone, Riccardo, additional, Vanoli, Alessandro, additional, Vitale, Serena, additional, Volta, Umberto, additional, Zanini, Annalisa, additional, and Zingone, Fabiana, additional
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- 2024
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22. Medical treatments and follow-up for clinical conditions associated to celiac disease
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Gasbarrini, Antonio, primary, Bibbò, Stefano, additional, Ianiro, Gianluca, additional, Cammarota, Giovanni, additional, and Gasbarrini, Giovanni, additional
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- 2024
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23. Corrigendum: Biosecurity measures to control hepatitis E virus on European pig farms
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Tamino Dubbert, Marina Meester, Richard Piers Smith, Tijs J. Tobias, Ilaria Di Bartolo, Reimar Johne, Enrico Pavoni, Gergana Krumova-Valcheva, Elena Lucia Sassu, Christopher Prigge, Giuseppe Aprea, Hannah May, Nadine Althof, Giovanni Ianiro, Jacek Żmudzki, Albena Dimitrova, Giovanni Loris Alborali, Daniela D'Angelantonio, Silvia Scattolini, Noemi Battistelli, and Elke Burow
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hepatitis-E-virus ,HEV ,biosecurity measures ,pig production ,BIOPIGEE ,One Health ,Veterinary medicine ,SF600-1100 - Published
- 2024
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24. An end-to-end KNN-based PTV approach for high-resolution measurements and uncertainty quantification
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Tirelli, Iacopo, Ianiro, Andrea, and Discetti, Stefano
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Physics - Fluid Dynamics - Abstract
We introduce a novel end-to-end approach to improving the resolution of PIV measurements. The method blends information from different snapshots without the need for time-resolved measurements on grounds of similarity of flow regions in different snapshots. The main hypothesis is that, with a sufficiently large ensemble of statistically-independent snapshots, the identification of flow structures that are morphologically similar but occurring at different time instants is feasible. Measured individual vectors from different snapshots with similar flow organisation can thus be merged, resulting in an artificially increased particle concentration. This allows to refine the interrogation region and, consequently, increase the spatial resolution. The measurement domain is split in subdomains. The similarity is enforced only on a local scale, i.e. morphologically-similar regions are sought only among subdomains corresponding to the same flow region. The identification of locally-similar snapshots is based on unsupervised K-nearest neighbours search in a space of significant flow features. Such features are defined in terms of a Proper Orthogonal Decomposition, performed in subdomains on the original low-resolution data, obtained either with standard cross-correlation or with binning of Particle Tracking Velocimetry data with a relatively large bin size. A refined bin size is then selected according to the number of "sufficiently close" snapshots identified. The statistical dispersion of the velocity vectors within the bin is then used to estimate the uncertainty and to select the optimal K which minimises it. The method is tested and validated against datasets with a progressively increasing level of complexity: two virtual experiments based on direct simulations of the wake of a fluidic pinball and a channel flow and the experimental data collected in a turbulent boundary layer.
- Published
- 2022
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25. Heat transfer enhancement in turbulent boundary layers with a pulsed slot jet in crossflow
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Castellanos, Rodrigo, Salih, Gianfranco, Raiola, Marco, Ianiro, Andrea, and Discetti, Stefano
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Physics - Fluid Dynamics - Abstract
The convective heat transfer enhancement in a turbulent boundary layer (TBL) employing a pulsed, slot jet in crossflow is investigated experimentally. A parametric study on actuation frequencies and duty cycles is performed. The actuator is a flush-mounted slot jet that injects fluid into a well-behaved zero-pressure-gradient TBL over a flat plate. A heated-thin-foil sensor measures the time-averaged convective heat transfer coefficient downstream of the actuator location and the flow field is characterised by means of Particle Image Velocimetry. The results show that both the jet penetration in the streamwise direction and the overall Nusselt number increase with increasing duty cycle. The frequency at which the Nusselt number is maximised is independent of the duty cycle. The flow topology is considerably altered by the jet pulsation. A wall-attached jet rises from the slot accompanied by a pair of counter-rotating vortices that promote flow entrainment and mixing. Eventually, a simplified model is proposed which decouples the effect of pulsation frequency and duty cycle in the overall heat transfer enhancement, with a good agreement with experimental data. The cost of actuation is also quantified in terms of the amount of injected fluid during the actuation, leading to conclude that the lowest duty cycle is the most efficient for heat transfer enhancement.
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- 2022
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26. From snapshots to manifolds - A tale of shear flows
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Farzamnik, Ehsan, Ianiro, Andrea, Discetti, Stefano, Deng, Nan, Oberleithner, Kilian, Noack, Bernd R., and Guerrero, Vanesa
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Physics - Fluid Dynamics - Abstract
We propose a novel non-linear manifold learning from snapshot data and demonstrate its superiority over Proper Orthogonal Decomposition (POD) for shedding-dominated shear flows. Key enablers are isometric feature mapping, Isomap (Tenenbaum et al., 2000), as encoder and K-nearest neighbours (KNN) algorithm as decoder. The proposed technique is applied to numerical and experimental datasets including the fluidic pinball, a swirling jet, and the wake behind a couple of tandem cylinders. Analyzing the fluidic pinball, the manifold is able to describe the pitchfork bifurcation and the chaotic regime with only three feature coordinates. These coordinates are linked to vortex-shedding phases and the force coefficients. The manifold coordinates of the swirling jet are comparable to the POD mode amplitudes, yet allow for a more distinct manifold identification which is less sensitive to measurement noise. As similar observation is made for the wake of two tandem cylinders (Raiola et al., 2016). The tandem cylinders are aligned in streamwise distance which corresponds to the transition between the single bluff body and the reattachment regimes of vortex shedding. Isomap unveils these two shedding regimes while the Lissajous plots of first two POD mode amplitudes feature a single circle. The reconstruction error of the manifold model is small compared to the fluctuation level, indicating that the low embedding dimensions contains the coherent structure dynamics. The proposed Isomap-KNN manifold learner is expected to be of large importance in estimation, dynamic modeling and control for large range of configurations with dominant coherent structures.
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- 2022
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27. Machine learning flow control with few sensor feedback and measurement noise
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Castellanos, R., Maceda, G. Y. Cornejo, de la Fuente, I., Noack, B. R., Ianiro, A., and Discetti, S.
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Physics - Fluid Dynamics - Abstract
A comparative assessment of machine learning (ML) methods for active flow control is performed. The chosen benchmark problem is the drag reduction of a two-dimensional K\'arm\'an vortex street past a circular cylinder at a low Reynolds number ($Re=100$). The flow is manipulated with two blowing/suction actuators on the upper and lower side of a cylinder. The feedback employs several velocity sensors. Two probe configurations are evaluated: 5 and 11 velocity probes located at different points around the cylinder and in the wake. The control laws are optimized with Deep Reinforcement Learning (DRL) and Linear Genetic Programming Control (LGPC). By interacting with the unsteady wake, both methods successfully stabilize the vortex alley and effectively reduce drag while using small mass flow rates for the actuation. DRL has shown higher robustness with respect to variable initial conditions and to noise contamination of the sensor data; on the other hand, LGPC is able to identify compact and interpretable control laws, which only use a subset of sensors, thus allowing reducing the system complexity with reasonably good results. Our study points at directions of future machine learning control combining desirable features of different approaches.
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- 2022
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28. Persistence of hepatitis E virus (HEV) subtypes 3c and 3e: Long-term cold storage and heat treatments
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Monini, Marina, Ianiro, Giovanni, De Sabato, Luca, Bivona, Marta, Ostanello, Fabio, and Di Bartolo, Ilaria
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- 2024
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29. A 1-year follow-up study on checkpoint inhibitor-induced colitis: results from a European consortium
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Mengoli, C., Aronico, N., Lepore, F., Broglio, G., Merli, S., Natalello, G., Alimenti, E., Scalvini, D., Muscarella, S., Agustoni, F., Pagani, A., Chiellino, S., Corallo, S., Musella, V., Cannizzaro, R., Vecchi, M., Caprioli, F., Gabbiadini, R., Dal Buono, A., Premoli, A., Locati, L.D., Buda, A., Contaldo, A., Schiepatti, A., Biagi, F., Morano, D., Cucè, M., Kotsakis, A., De Lisi, G., Lenti, M.V., Ribaldone, D.G., Borrelli de Andreis, F., Vernero, M., Barberio, B., De Ruvo, M., Savarino, E.V., Kav, T., Blesl, A., Franzoi, M., Gröchenig, H.P., Pugliese, D., Ianiro, G., Porcari, S., Cammarota, G., Gasbarrini, A., Spagnuolo, R., Ellul, P., Foteinogiannopoulou, K., Koutroubakis, I., Argyriou, K., Cappello, M., Jauregui-Amezaga, A., Demarzo, M.G., Silvestris, N., Armuzzi, A., Sottotetti, F., Bertani, L., Festa, S., Eder, P., Pedrazzoli, P., Lasagna, A., Vanoli, A., Gambini, G., Santacroce, G., Rossi, C.M., Delliponti, M., Klersy, C., Corazza, G.R., and Di Sabatino, A.
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- 2024
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30. Donor screening for fecal microbiota transplantation with a direct stool testing-based strategy: a prospective cohort study
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Rondinella, Debora, Quaranta, Gianluca, Rozera, Tommaso, Dargenio, Pasquale, Fancello, Giovanni, Venturini, Irene, Guarnaccia, Alessandra, Porcari, Serena, Bibbò, Stefano, Sanguinetti, Maurizio, Gasbarrini, Antonio, Masucci, Luca, Cammarota, Giovanni, and Ianiro, Gianluca
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- 2024
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31. Gemeinsam stark: Wie Peer-Trainings die digitale Kompetenz von Studierenden fördern
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Patrizia Ianiro-Dahm, Alexandra Reher, and Christine Syrek
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Education - Abstract
Die moderne Arbeitswelt erfordert digitale Kompetenz, doch Hochschulen mangelt es an Angeboten zum digitalen Kompetenzaufbau Studierender. Peer-Angebote können ein sinnvoller Ansatz zur Förderung digitaler Kompetenz sein, allerdings fehlen empirische Belege für deren Wirksamkeit. Die Studie setzt hier an und evaluiert den digitalen Kompetenzerwerb von Teilnehmenden fachübergreifender Peer-Trainings auf Grundlage des DigComp Rahmenmodells. Die Ergebnisse zeigen, dass Trainings-Teilnehmende ihre digitale Kompetenz im Vergleich zur Kontrollgruppe signifikant stärker steigern konnten. Die Ausbildung zur bzw. zum Peer-Trainer:in sowie die Peer-Trainings wurden von allen Beteiligten sehr positiv bewertet.
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- 2024
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32. Predicting the near-wall region of turbulence through convolutional neural networks
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Balasubramanian, A. G., Guastoni, L., Güemes, A., Ianiro, A., Discetti, S., Schlatter, P., Azizpour, H., and Vinuesa, R.
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Physics - Fluid Dynamics ,Statistics - Machine Learning - Abstract
Modelling the near-wall region of wall-bounded turbulent flows is a widespread practice to reduce the computational cost of large-eddy simulations (LESs) at high Reynolds number. As a first step towards a data-driven wall-model, a neural-network-based approach to predict the near-wall behaviour in a turbulent open channel flow is investigated. The fully-convolutional network (FCN) proposed by Guastoni et al. [preprint, arXiv:2006.12483] is trained to predict the two-dimensional velocity-fluctuation fields at $y^{+}_{\rm target}$, using the sampled fluctuations in wall-parallel planes located farther from the wall, at $y^{+}_{\rm input}$. The data for training and testing is obtained from a direct numerical simulation (DNS) at friction Reynolds numbers $Re_{\tau} = 180$ and $550$. The turbulent velocity-fluctuation fields are sampled at various wall-normal locations, i.e. $y^{+} = \{15, 30, 50, 80, 100, 120, 150\}$. At $Re_{\tau}=550$, the FCN can take advantage of the self-similarity in the logarithmic region of the flow and predict the velocity-fluctuation fields at $y^{+} = 50$ using the velocity-fluctuation fields at $y^{+} = 100$ as input with less than 20% error in prediction of streamwise-fluctuations intensity. These results are an encouraging starting point to develop a neural-network based approach for modelling turbulence at the wall in numerical simulations., Comment: Proc. 13th ERCOFTAC Symp. on Engineering Turbulence Modeling and Measurements (ETMM13), Rhodes, Greece, September 15-17, 2021
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- 2021
33. Watching Single Unmodified Enzymes at Work
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Ying, Cuifeng, Karakaci, Edona, Bermudez-Urena, Esteban, Ianiro, Alessandro, Foster, Ceri, Awasthi, Saurabh, Guha, Anirvan, Bryan, Louise, List, Jonathan, Balog, Sandor, Acuna, Guillermo P., Gordon, Reuven, and Mayer, Michael
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Physics - Biological Physics ,Physics - Optics ,Quantitative Biology - Biomolecules - Abstract
Many proteins undergo conformational changes during their activity. A full understanding of the function of these proteins can only be obtained if different conformations and transitions between them can be monitored in aqueous solution, with adequate temporal resolution and, ideally, on a single-molecule level. Interrogating conformational dynamics of single proteins remains, however, exquisitely challenging and typically requires site-directed chemical modification combined with rigorous minimization of possible artifacts. These obstacles limit the number of single-protein investigations. The work presented here introduces an approach that traps single unmodified proteins from solution in a plasmonic hotspot and makes it possible to assign changes in refractive index to changes in protein conformation while monitoring these changes for minutes to hours with a temporal resolution at least as fast as 40 microseconds. The resulting single molecule data reveals that adenylate kinase employs a hidden enzymatic sub-cycle during catalysis, that citrate synthase populates a previously unknown intermediate conformation, which is more important for its enzymatic activity than its well-known open conformation, that hemoglobin transitions in several steps from its deoxygenated and rigid T state to its oxygenated and flexible R state, and that apo-calmodulin thermally unfolds and refolds in steps that correspond to conformational changes of individual protein domains., Comment: 20 pages, 4 figures
- Published
- 2021
34. Lactoferrin: from the structure to the functional orchestration of iron homeostasis
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Ianiro, Giusi, Rosa, Luigi, Bonaccorsi di Patti, Maria Carmela, Valenti, Piera, Musci, Giovanni, and Cutone, Antimo
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- 2023
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35. Fine-tuning the gut ecosystem: the current landscape and outlook of artificial microbiome therapeutics
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Porcari, Serena, Fusco, William, Spivak, Igor, Fiorani, Marcello, Gasbarrini, Antonio, Elinav, Eran, Cammarota, Giovanni, and Ianiro, Gianluca
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- 2024
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36. Investigation of Salmonella, hepatitis E virus (HEV) and viral indicators of fecal contamination in four Italian pig slaughterhouses, 2021–2022
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Ianiro, Giovanni, Pavoni, Enrico, De Sabato, Luca, Monini, Marina, Delibato, Elisabetta, Perrone, Vitantonio, Ostanello, Fabio, Niine, Tarmo, and Di Bartolo, Ilaria
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- 2024
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37. Inter-institutional variability of knowledge-based plan prediction of left whole breast irradiation
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Castriconi, Roberta, Tudda, Alessia, Placidi, Lorenzo, Benecchi, Giovanna, Cagni, Elisabetta, Dusi, Francesca, Ianiro, Anna, Landoni, Valeria, Malatesta, Tiziana, Mazzilli, Aldo, Meffe, Guenda, Oliviero, Caterina, Rambaldi Guidasci, Giulia, Scaggion, Alessandro, Trojani, Valeria, del Vecchio, Antonella, and Fiorino, Claudio
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- 2024
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38. From coarse wall measurements to turbulent velocity fields through deep learning
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Güemes, Alejandro, Discetti, Stefano, Ianiro, Andrea, Sirmacek, Beril, Azizpour, Hossein, and Vinuesa, Ricardo
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Physics - Fluid Dynamics - Abstract
This work evaluates the applicability of super-resolution generative adversarial networks (SRGANs) as a methodology for the reconstruction of turbulent-flow quantities from coarse wall measurements. The method is applied both for the resolution enhancement of wall fields and the estimation of wall-parallel velocity fields from coarse wall measurements of shear stress and pressure. The analysis has been carried out with a database of a turbulent open-channel flow with friction Reynolds number $Re_{\tau}=180$ generated through direct numerical simulation. Coarse wall measurements have been generated with three different downsampling factors $f_d=[4,8,16]$ from the high-resolution fields, and wall-parallel velocity fields have been reconstructed at four inner-scaled wall-normal distances $y^+=[15,30,50,100]$. We first show that SRGAN can be used to enhance the resolution of coarse wall measurements. If compared with direct reconstruction from the sole coarse wall measurements, SRGAN provides better instantaneous reconstructions, both in terms of mean-squared error and spectral-fractional error. Even though lower resolutions in the input wall data make it more challenging to achieve highly accurate predictions, the proposed SRGAN-based network yields very good reconstruction results. Furthermore, it is shown that even for the most challenging cases the SRGAN is capable of capturing the large-scale structures that populate the flow. The proposed novel methodology has great potential for closed-loop control applications relying on non-intrusive sensing.
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- 2021
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39. Valpalf®: A New Nutraceutical Formulation Containing Bovine Lactoferrin That Exhibits Potentiated Biological Activity
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Luigi Rosa, Giusi Ianiro, Antonella Niro, Giovanni Musci, Rosalba Paesano, Antimo Cutone, and Piera Valenti
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bovine lactoferrin ,Valpalf® ,iron chelation ,proteolysis resistance ,antioxidant activity ,anti-inflammatory activity ,Biology (General) ,QH301-705.5 ,Chemistry ,QD1-999 - Abstract
As a nutraceutical, bovine lactoferrin (bLf), an iron-binding glycoprotein involved in innate immunity, is gaining elevated attention for its ability to exert pleiotropic functions and to be exceptionally tolerated even at high dosages. Some of bLf’s activities, including its anti-inflammatory and antioxidant, are tightly linked to its ability to both chelate iron and enter inside the cell nucleus. Here, we present data about Valpalf®, a new formulation containing bLf, sodium citrate, and sodium bicarbonate at a molar ratio of 10−3. In the present study, Valpalf® exhibits superior iron-binding capacity, resistance to tryptic digestion, and a greater capacity to accumulate into the nucleus over time when compared to the native bLf alone. In agreement, Valpalf® effectively reduces interleukin(IL)-6 levels in lipopolysaccharide-stimulated macrophages and modulates the expression of antioxidant enzymes, such as superoxide dismutase 1 and 2, in phorbol-12-myristate-13-acetate-stimulated monocytes. Of note, this potentiated bioactivity was corroborated in a retrospective study on the treatment of anemia of inflammation in hereditary thrombophilic pregnant and non-pregnant women, demonstrating that Valpalf® improves hematological parameters and reduces serum IL-6 levels to a higher extent than bLf alone.
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- 2024
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40. Investigating the Hepatitis E Virus (HEV) Diversity in Rat Reservoirs from Northern Italy
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Luca De Sabato, Marina Monini, Roberta Galuppi, Filippo Maria Dini, Giovanni Ianiro, Gabriele Vaccari, Fabio Ostanello, and Ilaria Di Bartolo
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hepatitis E virus ,reservoir ,rat ,zoonoses ,HEV-C1 ,Italy ,Medicine - Abstract
Hepatitis E virus belonging to the Rocahepevirus ratti species, genotype HEV-C1, has been extensively reported in rats in Europe, Asia and North America. Recently, human cases of hepatitis associated with HEV-C1 infection have been reported, but the zoonotic nature of rat-HEV remains controversial. The transmission route of rat-HEV is unidentified and requires further investigation. The HEV strains of the Paslahepevirus balayani species, belonging to the same Hepeviridae family, and including the zoonotic genotype HEV-3 usually found in pigs, have also sporadically been identified in rats. We sampled 115 rats (liver, lung, feces) between 2020 and 2023 in Northeast Italy and the HEV detection was carried out by using Reverse Transcription PCR. HEV RNA was detected in 3/115 (2.6%) rats who tested positive for HEV-C1 strains in paired lung, intestinal contents and liver samples. Overall, none tested positive for the Paslahepevirus balayani strains. In conclusion, our results confirm the presence of HEV-rat in Italy with a prevalence similar to previous studies but show that there is a wide heterogeneity of strains in circulation. The detection of HEV-C1 genotype of Rocahepevirus ratti species in some human cases of acute hepatitis suggests that HEV-C1 may be an underestimated source of human infections. This finding, with the geographically widespread detection of HEV-C1 in rats, raises questions about the role of rats as hosts for both HEV-C1 and HEV-3 and the possibility of zoonotic transmission.
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- 2024
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41. Exploring the Potential of Muridae as Sentinels for Human and Zoonotic Viruses
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Ilaria Di Bartolo, Luca De Sabato, Giovanni Ianiro, Gabriele Vaccari, Filippo Maria Dini, Fabio Ostanello, and Marina Monini
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Muridae ,rats ,mice ,viral zoonoses ,rodents ,reservoir ,Microbiology ,QR1-502 - Abstract
In recent years, the transmission of viruses from wildlife to humans has raised significant public health concerns, exemplified by the COVID-19 pandemic caused by the betacoronavirus SARS-CoV-2. Human activities play a substantial role in increasing the risk of zoonotic virus transmission from wildlife to humans. Rats and mice are prevalent in urban environments and may act as reservoirs for various pathogens. This study aimed to evaluate the presence of zoonotic viruses in wild rats and mice in both urban and rural areas, focusing on well-known zoonotic viruses such as betacoronavirus, hantavirus, arenavirus, kobuvirus, and monkeypox virus, along with other viruses occasionally detected in rats and mice, including rotavirus, norovirus, and astrovirus, which are known to infect humans at a high rate. A total of 128 animals were captured, including 70 brown rats (Rattus norvegicus), 45 black rats (Rattus rattus), and 13 house mice (Mus musculus), and feces, lung, and liver were collected. Among brown rats, one fecal sample tested positive for astrovirus RNA. Nucleotide sequencing revealed high sequence similarity to both human and rat astrovirus, suggesting co-presence of these viruses in the feces. Murine kobuvirus (MuKV) was detected in fecal samples from both black (n = 7) and brown (n = 6) rats, primarily from urban areas, as confirmed by sequence analysis. These findings highlight the importance of surveillance and research to understand and mitigate the risks associated with the potential transmission of pathogens by rodents.
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- 2024
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42. Helicobacter pylori and the Human Gastrointestinal Microbiota: A Multifaceted Relationship
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Ege Tohumcu, Francesco Kaitsas, Ludovica Bricca, Alessandro Ruggeri, Antonio Gasbarrini, Giovanni Cammarota, and Gianluca Ianiro
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gastritis ,Helicobacter pylori ,gut and gastric microbiota ,H. pylori eradication therapies ,Therapeutics. Pharmacology ,RM1-950 - Abstract
Helicobacter pylori is a type of Gram-negative bacteria belonging to the Proteobacteria phylum which is known to cause gastrointestinal disorders such as gastritis and gastric ulcers. Its treatment is based on current eradication regimens, which are composed of combinations of antibiotics such as clarithromycin, metronidazole, levofloxacin and amoxicillin, often combined with a proton pump inhibitor (PPI). With the development of sequencing technologies, it has been demonstrated that not only does the colonization of the gastric and gut environment by H. pylori cause microbial changes, but also the treatment regimens used for its eradication have a significant altering effect on both the gastric and gut microbiota. Here, we review current knowledge on microbiota modulations of current therapies in both environments. We also summarize future perspectives regarding H. pylori infection, the integration of probiotics into therapy and what challenges are being faced on a global basis when we talk about eradication.
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- 2024
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43. The role of microbiome in the development of gluten-related disorders
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Catassi, Giulia, Lener, Elena, Grattagliano, Maria Maddalena, Motuz, Sofya, Zavarella, Maria Antonietta, Bibbò, Stefano, Cammarota, Giovanni, Gasbarrini, Antonio, Ianiro, Gianluca, and Catassi, Carlo
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- 2024
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44. Gut microbiota in colorectal cancer: From pathogenesis to clinic
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Fusco, William, Bricca, Ludovica, Kaitsas, Francesco, Tartaglia, Maria Francesca, Venturini, Irene, Rugge, Massimo, Gasbarrini, Antonio, Cammarota, Giovanni, and Ianiro, Gianluca
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- 2024
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45. The microbiome-driven impact of western diet in the development of noncommunicable chronic disorders
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Severino, Andrea, Tohumcu, Ege, Tamai, Luca, Dargenio, Pasquale, Porcari, Serena, Rondinella, Debora, Venturini, Irene, Maida, Marcello, Gasbarrini, Antonio, Cammarota, Giovanni, and Ianiro, Gianluca
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- 2024
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46. Common and Rare Variants in TMEM175 Gene Concur to the Pathogenesis of Parkinson’s Disease in Italian Patients
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Palomba, Nicole Piera, Fortunato, Giorgio, Pepe, Giuseppe, Modugno, Nicola, Pietracupa, Sara, Damiano, Immacolata, Mascio, Giada, Carrillo, Federica, Di Giovannantonio, Luca Giovanni, Ianiro, Laura, Martinello, Katiuscia, Volpato, Viola, Desiato, Vincenzo, Acri, Riccardo, Storto, Marianna, Nicoletti, Ferdinando, Webber, Caleb, Simeone, Antonio, Fucile, Sergio, Maglione, Vittorio, and Esposito, Teresa
- Published
- 2023
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47. Biosecurity measures to control hepatitis E virus on European pig farms
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Tamino Dubbert, Marina Meester, Richard Piers Smith, Tijs J. Tobias, Ilaria Di Bartolo, Reimar Johne, Enrico Pavoni, Gergana Krumova-Valcheva, Elena Lucia Sassu, Christopher Prigge, Giuseppe Aprea, Hannah May, Nadine Althof, Giovanni Ianiro, Jacek Żmudzki, Albena Dimitrova, Giovanni Loris Alborali, Daniela D'Angelantonio, Silvia Scattolini, Noemi Battistelli, and Elke Burow
- Subjects
hepatitis-E-virus ,HEV ,biosecurity measures ,pig production ,BIOPIGEE ,One Health ,Veterinary medicine ,SF600-1100 - Abstract
Hepatitis E virus (HEV) genotype 3 is a prevalent zoonotic pathogen in European pig farms, posing a significant public health risk primarily through the foodborne route. The study aimed to identify effective biosecurity measures for controlling HEV transmission on pig farms, addressing a critical gap in current knowledge. Utilizing a cross-sectional design, fecal samples from gilts, dry sows, and fatteners were collected on 231 pig farms of all farm types across nine European countries. Real-time RT-PCR was employed to test these samples for HEV. Simultaneously, a comprehensive biosecurity questionnaire captured data on various potential measures to control HEV. The dependent variable was HEV risk, categorized as lower or higher based on the percentage of positive pooled fecal samples on each farm (25% cut-off). The data were analyzed using generalized linear models (one for finisher samples and one for all samples) with a logit link function with country and farm type as a priori fixed factors. The results of the final multivariable models identified key biosecurity measures associated with lower HEV risk, which were the use of a hygienogram in the breeding (OR: 0.06, p = 0.001) and/or fattening area after cleaning (OR: 0.21, p = 0.019), the presence of a quarantine area (OR: 0.29, p = 0.025), testing and/or treating purchased feed against Salmonella (OR: 0.35, p = 0.021), the presence of other livestock species on the farm, and having five or fewer persons in charge of the pigs. Contrary to expectations, some biosecurity measures were associated with higher HEV risk, e.g., downtime of 3 days or longer after cleaning in the fattening area (OR: 3.49, p = 0.005) or mandatory handwashing for farm personnel when changing barn sections (OR: 3.4, p = 0.026). This novel study unveils critical insights into biosecurity measures effective in controlling HEV on European pig farms. The identification of both protective and risk-associated measures contributes to improving strategies for managing HEV and underscores the complexity of biosecurity in pig farming.
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- 2024
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48. Effects of natural treatments on the varroa mite infestation levels and overall health of honey bee (Apis mellifera) colonies
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Laura Narciso, Martina Topini, Sonia Ferraiuolo, Giovanni Ianiro, and Cinzia Marianelli
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Medicine ,Science - Published
- 2024
49. Convolutional-network models to predict wall-bounded turbulence from wall quantities
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Guastoni, L., Güemes, A., Ianiro, A., Discetti, S., Schlatter, P., Azizpour, H., and Vinuesa, R.
- Subjects
Physics - Fluid Dynamics ,Physics - Computational Physics ,Statistics - Machine Learning - Abstract
Two models based on convolutional neural networks are trained to predict the two-dimensional velocity-fluctuation fields at different wall-normal locations in a turbulent open channel flow, using the wall-shear-stress components and the wall pressure as inputs. The first model is a fully-convolutional neural network (FCN) which directly predicts the fluctuations, while the second one reconstructs the flow fields using a linear combination of orthonormal basis functions, obtained through proper orthogonal decomposition (POD), hence named FCN-POD. Both models are trained using data from two direct numerical simulations (DNS) at friction Reynolds numbers $Re_{\tau} = 180$ and $550$. Thanks to their ability to predict the nonlinear interactions in the flow, both models show a better prediction performance than the extended proper orthogonal decomposition (EPOD), which establishes a linear relation between input and output fields. The performance of the various models is compared based on predictions of the instantaneous fluctuation fields, turbulence statistics and power-spectral densities. The FCN exhibits the best predictions closer to the wall, whereas the FCN-POD model provides better predictions at larger wall-normal distances. We also assessed the feasibility of performing transfer learning for the FCN model, using the weights from $Re_{\tau}=180$ to initialize those of the $Re_{\tau}=550$ case. Our results indicate that it is possible to obtain a performance similar to that of the reference model up to $y^{+}=50$, with $50\%$ and $25\%$ of the original training data. These non-intrusive sensing models will play an important role in applications related to closed-loop control of wall-bounded turbulence., Comment: 31 pages, 17 figures
- Published
- 2020
50. Gut microbiota functional profiling in autism spectrum disorders: bacterial VOCs and related metabolic pathways acting as disease biomarkers and predictors
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Pamela Vernocchi, Chiara Marangelo, Silvia Guerrera, Federica Del Chierico, Valerio Guarrasi, Simone Gardini, Federica Conte, Paola Paci, Gianluca Ianiro, Antonio Gasbarrini, Stefano Vicari, and Lorenza Putignani
- Subjects
autism ,gut microbiota volatilome ,tryptophan-derived metabolism ,SCFAs ,machine learning ,clinical decision support system (CDSS) algorithms ,Microbiology ,QR1-502 - Abstract
BackgroundAutism spectrum disorder (ASD) is a multifactorial neurodevelopmental disorder. Major interplays between the gastrointestinal (GI) tract and the central nervous system (CNS) seem to be driven by gut microbiota (GM). Herein, we provide a GM functional characterization, based on GM metabolomics, mapping of bacterial biochemical pathways, and anamnestic, clinical, and nutritional patient metadata.MethodsFecal samples collected from children with ASD and neurotypical children were analyzed by gas-chromatography mass spectrometry coupled with solid phase microextraction (GC–MS/SPME) to determine volatile organic compounds (VOCs) associated with the metataxonomic approach by 16S rRNA gene sequencing. Multivariate and univariate statistical analyses assessed differential VOC profiles and relationships with ASD anamnestic and clinical features for biomarker discovery. Multiple web-based and machine learning (ML) models identified metabolic predictors of disease and network analyses correlated GM ecological and metabolic patterns.ResultsThe GM core volatilome for all ASD patients was characterized by a high concentration of 1-pentanol, 1-butanol, phenyl ethyl alcohol; benzeneacetaldehyde, octadecanal, tetradecanal; methyl isobutyl ketone, 2-hexanone, acetone; acetic, propanoic, 3-methyl-butanoic and 2-methyl-propanoic acids; indole and skatole; and o-cymene. Patients were stratified based on age, GI symptoms, and ASD severity symptoms. Disease risk prediction allowed us to associate butanoic acid with subjects older than 5 years, indole with the absence of GI symptoms and low disease severity, propanoic acid with the ASD risk group, and p-cymene with ASD symptoms, all based on the predictive CBCL-EXT scale. The HistGradientBoostingClassifier model classified ASD patients vs. CTRLs by an accuracy of 89%, based on methyl isobutyl ketone, benzeneacetaldehyde, phenyl ethyl alcohol, ethanol, butanoic acid, octadecane, acetic acid, skatole, and tetradecanal features. LogisticRegression models corroborated methyl isobutyl ketone, benzeneacetaldehyde, phenyl ethyl alcohol, skatole, and acetic acid as ASD predictors.ConclusionOur results will aid the development of advanced clinical decision support systems (CDSSs), assisted by ML models, for advanced ASD-personalized medicine, based on omics data integrated into electronic health/medical records. Furthermore, new ASD screening strategies based on GM-related predictors could be used to improve ASD risk assessment by uncovering novel ASD onset and risk predictors.
- Published
- 2023
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- View/download PDF
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