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Soil microbial communities impact carbon sequestration and release, biogeochemical cycling, and agricultural yields. These global effects rely on metabolic interactions that modulate community composition and function. However, the physicochemical and taxonomic complexity of soil and the scarcity of available isolates for phenotypic testing are significant barriers to studying soil microbial interactions. Corrinoids-the vitamin B12 family of cofactors-are critical for microbial metabolism, yet they are synthesized by only a subset of microbiome members. Here, we evaluated corrinoid production and dependence in soil bacteria as a model to investigate the ecological roles of microorganisms involved in metabolic interactions. We isolated and characterized a taxonomically diverse collection of 161 soil bacteria from a single study site. Most corrinoid-dependent bacteria in the collection prefer B12 over other corrinoids, while all tested producers synthesize B12, indicating metabolic compatibility between producers and dependents in the collection. Furthermore, a subset of producers release B12 at levels sufficient to support dependent isolates in laboratory culture at estimated ratios of up to 1000 dependents per producer. Within our isolate collection, we did not find strong phylogenetic patterns in corrinoid production or dependence. Upon investigating trends in the phylogenetic dispersion of corrinoid metabolism categories across sequenced bacteria from various environments, we found that these traits are conserved in 47 out of 85 genera. Together, these phenotypic and genomic results provide evidence for corrinoid-based metabolic interactions among bacteria and provide a framework for the study of nutrient-sharing ecological interactions in microbial communities.

ABSTRACT Aim Location Taxon Methods Results Main Conclusion We assessed the influence of island isolation on the composition of insular bird assemblages with a particular focus on species traits associated with dispersal. To do so, we tested whether ecomorphological metrics of dispersal ability, namely hand‐wing index and Kipp's distance, increase with increasing island isolation.Global.Birds.We integrated global datasets of island characteristics with distribution and ecomorphological trait information of birds; our final dataset comprised information for 2034 native, resident and terrestrial species inhabiting 2399 islands. Species restricted to islands were removed to avoid potentially confounding effects of speciation, such as the evolution of flightlessness or poor flight on islands. Using the generalised additive models, we tested for the relationship between hand‐wing index or Kipp's distance and island isolation, accounting for the effects of island area and spatial autocorrelation. We performed the analyses separately for (i) continental and oceanic islands and (ii) for all terrestrial birds and for passerine birds only.Hand‐wing index and Kipp's distance were positively related to island isolation on oceanic islands, that is bird communities on more isolated oceanic islands were composed of species with wings that had a higher aspect ratio and were more elongated. However, this relationship did not hold for continental islands. We found these patterns to be consistent for all terrestrial birds as well as for passerine birds only.Our study provides strong evidence that island isolation influences the trait composition of island bird assemblages at a global scale. Our results highlight the variation of dispersal‐related ecomorphological traits among bird assemblages on islands, suggesting that these traits play an important role in mediating the influence of island isolation on community assembly processes on islands. [ABSTRACT FROM AUTHOR]

In this work, the rapid and efficient preparation of isolated galloyl glucoside tautomer free radical inhibitors was investigated using Saxifraga tangutica as a raw material. Four highly polar galloyl glucoside tautomers, 3-O-galloyl-α-d-glucose ⇌ 3-O-galloyl-β-d-glucose (Fr2-1-1), 2-O-galloyl-α-d-glucose ⇌ 2-O-galloyl-β-d-glucose (Fr2-1-2/2-1-3), 1-O-galloyl-β-d-glucose (Fr2-2-1), and 6-O-galloyl-α-d-glucose ⇌ 6-O-galloyl-β-d-glucose (Fr2-3-1/Fr2-3-2), were obtained via two-step medium-pressure liquid chromatography (with solid loading instead of conventional liquid injection) and one-step high-performance chromatography coupled with on-line RPLC-DPPH techniques for targeted isolation. This separation integration technique not only increases sample intake and reduces time cost but also visualizes each step of targeted separation. All four compounds were isolated from the plant for the first time. In vitro antioxidant activity assays by DPPH (1,1‑diphenyl-2-picrylhydrazyl) revealed that Fr2-1-2/Fr2-1-3 (IC50: 5.52 ± 0.32 μM), Fr2-2-1 (IC50: 7.22 ± 0.57 μM), and Fr2-3–1/Fr2-3-2 (IC50: 7.36 ± 0.25 μM) had superior free radical scavenging abilities and that both were superior to that of quercetin (IC50: 18.61 ± 3.55 μM). Oxidative stress assays revealed that Fr2-1-2/Fr2-1-3 significantly inhibited oxidative stress damage in H2O2-induced HepG2 cells, decreased the level of ROS (P < 0.01) and protected hepatocytes. Combined with the current results, gallic acid showed greater antioxidant activity when H atoms were replaced at d-glucose –OH (C-2) than at the other three sites [–OH (C-1), –OH (C-6) and –OH (C-3)]. [ABSTRACT FROM AUTHOR]

Summary: The present communication presents a novel compact microstrip line feed dual‐band four‐element MIMO (multiple input multiple output) antenna for 5G sub‐6GHz N79 and WiFi‐6E bands with a defined size of 48 × 48 × 1.6 mm3. The FR4 substrate used in the construction of the designated antenna has a thickness of 1.6 mm, a relative permittivity of 4.4, and loss tangent values of 0.02. The proposed MIMO architecture consists of four equilateral triangle‐shaped antenna elements on top and ground with inverted L‐shaped stubs on the substrate's bottom layer. The ground structure of the antenna is a connected ground and is formed by defected ground parts which are connected with inverted L‐shaped stubs. The orthogonal structure of the MIMO antenna elements, with four armed square stubs among them, enhanced the isolation between surrounding antennas. The proposed four‐port MIMO antenna is built and tested in to validate design, scattering, and radiation performance, as well as MIMO diversity features. The designed antenna has more than 15 dB isolation and operates at 4.75 GHz from 4.45 to 5.2 GHz (5G sub‐6 GHz N79 band) and 6.3 GHz from 5.95 to 7.15 GHz (WiFi‐6E band). Furthermore, the design provides omnidirectional patterns, high gain (6.5dBi) and efficiency (>95%), a low envelope correlation coefficient (<0.00001) and acceptable diversity gain (10 dB), a good total active refraction coefficient (−10 dB) and low channel capacity loss (0.03 bit/s/Hz), and a considerable mean effective gain (MEG = −3 dB) and MEG ratio (0 dB). Because the simulated and tested results match so well, the antenna is a good candidate for 5G sub‐6 GHz N79 band operations. [ABSTRACT FROM AUTHOR]

The persistence of small populations is influenced by the degree and cost of inbreeding, with the degree of inbreeding depending on whether close-kin mating is passively or actively avoided. Few studies have simultaneously studied these factors. We examined inbreeding in a small, isolated population of westslope cutthroat trout using extensive genetic and demographic data. Passive inbreeding avoidance was low, with predicted lifetime dispersal of approximately 36 and 74 m for females and males, respectively. Additionally, we found limited evidence for active inbreeding avoidance during reproduction. Relatives remained spatially clustered into adulthood, and observed relatedness among mate pairs was greater than expected under random mating by 0.09, suggesting that inbreeding is a concern in this population. Further, we examined sex-specific inbreeding depression throughout the life cycle and provide evidence for inbreeding depression in some fitness components, including family size, juvenile survival and reproductive success. Our results suggest that, in an at-risk trout population, limited passive and active inbreeding avoidance lead to a higher degree of inbreeding than expected under random mating. Observed inbreeding, along with evidence for fitness reduction due to inbreeding depression, could put the population at a heightened risk of decline or extirpation. [ABSTRACT FROM AUTHOR]

Bovine rotavirus (BRV) is the main cause of acute gastroenteritis in calves, resulting in significant economic losses to the cattle industry worldwide. Additionally, BRV has multiple genotypes, which could enable cross-species transmission, thereby posing a significant risk to public health. However, there is a problem of multiple genotypes coexisting in BRV, and the cross-protection effect between different genotypes of rotavirus strains is not effective enough. Therefore, mastering clinical epidemic genotypes and using epidemic genotype strains for vaccine preparation is an effective means of preventing and controlling BRV. In this study, BRV strain DQ2020 in MA104 cells was identified by transmission electron microscopy (TEM), reverse transcription polymerase chain reaction (RT-PCR), and colloidal gold immunochromatographic test strips. The whole genome of BRV strain DQ2020 was sequenced and pathogenicity in suckling mice was assessed. The results showed that after 10 passages in MA104 cells, BRV strain DQ2020 induced cytopathic effects. Wheel-shaped virus particles (diameter, ~80 nm) were observed by TEM. A target band of 382 bp was detected by RT-PCR, a positive band was detected with the colloidal gold immunochromatographic test strips, and significant green fluorescence was observed by indirect immunofluorescence (IFA). The highest median tissue culture infectious dose of strain DQ2020 after 9 passages in MA104 cells was 10−4.81 viral particles/0.1 mL. Based on phylogenetic analysis of 11 gene fragments, the genotype of BRV strain DQ2020 was G6-P[1]-I2-R2-C2-M2-A11-N2-T6-E2-H3, confirming transmission of the G6-P[1] genotype in Chinese cattle herds. Further analysis showed that the isolated strain was a reassortant of bovine (VP7, VP6, NSP3, and NSP5), human (VP4, VP1, VP2, VP3, NSP2, and NSP4), and ovine (NSP1) rotaviruses. BRV strain DQ2020 caused damage to the intestinal villi of suckling mice and diarrhea, confirming pathogenicity. In summary, this study identified a reassortant strain of bovine, human, and ovine rotavirus that is pathogenic to lactating mice, and conducted whole genome sequence analysis, providing valuable insights for the genetic evolution of the virus and the development of vaccines. [ABSTRACT FROM AUTHOR]

ABSTRACT A compact eight‐port MIMO structure containing planar inverted‐F antennas (PIFAs) as radiating elements is designed for 5G cellular communication applications within the sub‐6 GHz band. The proposed antenna consists of four radiating elements that are placed at four corners on the same plane of geometry. The overall dimension of the ground plane is considered to be 59 mm × 120 mm to make it convenient for modern smart mobile handsets. Each radiating element has 2‐feeding plates, which are situated perpendicular to each other in orientation to make the arrangement cross‐polarized, which in turn exploits polarization diversity as well as spatial diversity among the combination of different radiating elements. The patches are structured by embedding rectangular slots in a meandered shape, and the ground plane is configured by introducing rectangular slots and narrow metallic strips. The prototype of the prescribed MIMO PIFA model has been fabricated and experimentally tested. It shows multi‐band operations (3.27–3.37, 4.16–4.35, 4.93–5.06, and 5.47–5.68 GHz) within the sub‐6 GHz spectrum. The isolation among various patches is observed within the range of −35 dB to −42 dB. The peak gain reaches around 8.1 dBi. The designed MIMO PIFA exhibits superior diversity performance by producing ECC ≤ 0.0006, DG ≈ 10 dB, CCL < 0.20 bits/Hz/s, and MEG ≤ −3 dB. Furthermore, the specific absorption rate (SAR) is evaluated according to the standard values of fat, skin, and muscle at 3.3 GHz. The prescribed model maintains acceptable SAR values for 1‐g and 10‐g tissues, which makes it suitable for smartphone applications. [ABSTRACT FROM AUTHOR]

• Exploration of under-utilized Bambara groundnut as a potential source of protein for novel functional products. • Its isolation, characterization and utilization in food matrices also provides complete overview to enhance its functionality. • Future prospects in utilizing potential of BG proteins. Bambara groundnut (BG) is a particularly valuable underutilized crop indigenous to Africa that has gained popularity due to its high adaptation to climate change, drought tolerance and pest and disease resistance. It is chiefly cultivated as a plant-based protein alternative (containing 18–30 % protein) to meat in rural cuisines rather than an oil-producing seed, as commonly seen with other legumes. BG contains all the essential and non-essential amino acids in sufficient quantities to fulfil dietary and functional requirements in diverse food systems. This review delves into the techniques employed for the extraction/ isolation of BG proteins and their characterization. Different methods for the hydroxylation of BG proteins have been comprehensively reviewed and the relation between biological activity of BG peptides and amino acid composition has also been established. Comprehensive insights are given into the diverse techno-functional attributes of legume proteins, covering aspects like solubility, water and oil absorption capacity, emulsification, foaming, and gelling ability. The text also emphasizes the practical applications of these proteins across a range of products, viz. , bakery items, extruded snacks, therapeutic foods and desserts. New developments in BG protein research have been outlined along with recommendations for future research and applications, encouraging its widespread utilization across the food sector and facilitating a successful transition toward more sustainable plant-based diets. [ABSTRACT FROM AUTHOR]

Mesenchymal stem cells (MSCs) can be isolated from numerous tissues and have the potential for self-renewal and multidirectional differentiation. Evidence is accumulating which suggests that MSCs are also present in the gingival tissue. This study aimed to evaluate the feasibility of collecting, purifying, and amplifying gingival-derived MSCs (GMSCs) from canine gingiva and to obtain GMSC-derived exosomes (GMSC-exo). GMSCs were isolated and cultured; furthermore, cellular immunofluorescence demonstrated that GMSCs possess characteristic MSC markers, and in vitro differentiation was induced, indicating that GMSCs can differentiate into multiple lineages. GMSC-exo was successfully extracted from GMSCs supernatant and found that they exhibit the typical characteristics of exosomes as analyzed by transmission electron microscopy, nanoflow analysis, and western blotting. GMSC-exo promoted the proliferation and migration of Madin–Darby canine kidney cells. It was concluded that canine gingiva is a good source of MSCs. Additionally, GMSC-exo is a potentially promising cell-free therapeutic tool for the treatment of canine gingival diseases. [ABSTRACT FROM AUTHOR]

A positive association has been demonstrated between social supports, quality of life, and survival outcomes in cancer. This study assessed levels of social supports among patients with cancer in an Irish institution, with an age- and gender-specific stratification. The study highlights relatively low levels of perceived socio-emotional support and social connectedness, but good levels of tangible and informational support in our cohort of patients with cancer. Cancer clinicians should consider social supports as a factor when deciding upon cancer therapies and surveillance programs, and link in available support services for individuals with low levels of social supports where feasible. [ABSTRACT FROM AUTHOR]

Applying isolation devices to nuclear power plant equipment can improve the equipment's seismic safety. When the equipment is connected to piping, however, its seismic safety can rather be decreased due to the increase in the relative displacement of the connected pipes. An emergency diesel generator (EDG) supplies power when a generator and an external power source in a nuclear power plant do not operate. Isolation devices can be installed to improve EDG's seismic safety. The seismic safety of EDG is generally evaluated considering only EDG and the isolation device. Since the piping connected to EDG is responsible for fuel supply and cooling, however, the seismic safety of EDG must be evaluated considering the piping as well. In this study, seismic fragility analyses were conducted according to the modeling of isolation devices for EDG equipped with the isolation device and the piping connected to EDG. Seismic response analyses were performed considering the fuel line in EDG to which the seismic isolation devices were applied, and seismic fragility curves were estimated based on the results of the seismic response analysis. The research results showed that the piping connected to EDG could be damaged earlier than EDG, indicating that proper piping system design and seismic safety assessment are required to apply isolation devices to EDG. [ABSTRACT FROM AUTHOR]

The isolates of lactic acid bacteria (LAB) from traditional fermented koumiss and homemade dry rye sourdough were examined for their potential to be used in bread production without baker's yeast. Among twenty isolates, three with superior fermentation ability and acidification capability were identified by 16S rRNA gene sequencing. Leuconostoc mesenteroides from koumiss, Levilactobacillus brevis from 48 h activated rye sourdough, and Leuconostoc citreum from rye sourdough extract were singly and mixed (1:1:1) inoculated into liquid sourdoughs and used for bread-making tests. Bread prepared with Saccharomyces cerevisiae was used as the control. The substitution of water with whey in LAB fermentation demonstrated lower pH and higher total titratable acidity values in the sourdough. The resulting bread's color parameters, textural properties, and sensory characteristics confirmed the suitability of the selected strains to produce bread without baker's yeast and highlighted the enhancement of new starter varieties. [ABSTRACT FROM AUTHOR]

Fruits are a very good source of various nutrients that can boost overall human health. In these days, the recovery of therapeutic compounds from different fruit wastes is trending in research, which might not only minimize the waste problem but also encounter a higher demand for various enzymes that could have antimicrobial properties against infectious diseases. The goal of this review is to focus on the recovery of therapeutic enzymes from fruit wastes and its present-day tendency for utilization. Here we discussed different parts of fruit waste, such as pulp, pomace, seed, kernel, peel, etc., that produce therapeutic enzymes like amylase, cellulose, lipase, laccase, pectinase, etc. These bioactive enzymes are present in different parts of fruit and could be used as therapeutics against various infectious diseases. This article provides a thorough knowledge compilation of therapeutic enzyme isolation from fruit waste on a single platform, distinctly informative, and significant review work on the topic that is envisioned to encourage further research ideas in these areas that are still under-explored. This paper explains the various aspects of enzyme isolation from fruit and vegetable waste and their biotherapeutic potential that could provide new insights into the development of biotherapeutics and attract the attention of researchers to enhance translational research magnitude further. [ABSTRACT FROM AUTHOR]

Introduction: Getah virus (GETV) is a multi-host virus found in pigs, horses, and blue foxes. Clinically, GETV can cause fever, diarrhea, and reproductive disorders in pigs, representing significant threats to pig breeding. At present, few studies have examined the pathogenicity of GETV in pigs of different ages. Methods: In the present study, a new strain, named GETV-QJ, was isolated from clinically ill pigs, and whole genome sequencing analysis was performed. Besides the pathogenicity of piglets and pregnant sows of this strain was further studied. Results: the results illustrated that the strain belonged to group III. The strain had 93.6%-96.3% homology with other subtypes, and its homology with the same subtype strain ranged 96.5%-99%. Further studies on the pathogenicity of the virus indicated that this strain caused severe diarrhea, fever, and intestinal and lung damage in 7-day-old piglets, resulting in their death. The piglet survival rate was 0%. In pregnant sows, this strain did not cause fever, death, or abortion, but it induced viremia, which affected the farrowing performance of sows and led to reduced piglet survival. Discussion: In this study, we isolated a highly virulent group III and comprehensively established a pathogenic model of GETV in piglets and sows, providing a reference and guidance for the prevention and control of this infection. [ABSTRACT FROM AUTHOR]

An isolating set in a graph is a set X of vertices such that every edge of the graph is incident with a vertex of X or its neighborhood. The isolation number of a graph, or equivalently the vertex-edge domination number, is the minimum number of vertices in an isolating set. Caro and Hansberg, and independently Żyliński, showed that the isolation number is at most one-third the order for every connected graph of order at least 6. We show that in fact all such graphs have three disjoint isolating sets. Further, using a family introduced by Lemańska, Mora, and Souto-Salorio, we determine all graphs with equality in the original bound. [ABSTRACT FROM AUTHOR]

Background: Currently, there is a lack of research on multi-drug resistant Pseudomonas aeruginosa (MDR-PA) isolation in bronchiectasis-related hemoptysis. The aim of this study to analyze the risk factors for recurrent hemoptysis following bronchial artery embolization (BAE) and compare the recurrent hemoptysis-free rates between MDR-PA, non-MDR-PA, and non-PA isolation. Methods: A retrospective study was performed of patients diagnosed with idiopathic bronchiectasis-related recurrent hemoptysis who underwent BAE at an university-affiliated hospital. Patients were categorized based on PA susceptibility tests into non-PA, non-MDR-PA, and MDR-PA groups. Univariate and multivariate Cox regression were conducted to identify independent risk factors for recurrent hemoptysis. The Kaplan-Meier curves was conducted to compare recurrent hemoptysis-free rates after BAE for non-PA, non-MDR-PA, and MDR-PA. Results: A total of 432 patients were included. 181 (41.90%) patients experienced recurrent hemoptysis during a median follow-up period of 25 months. MDR-PA isolation (adjusted hazard ratio (aHR) 2.120; 95% confidence interval (CI) [1.249, 3.597], p = 0.005) was identified as an independent risk factor for recurrent hemoptysis. Antibiotic treatment (aHR 0.666; 95% CI [0.476, 0.932], p = 0.018) reduced the risk of recurrent hemoptysis. The cumulative recurrent hemoptysis-free rates for non-PA, non-MDR-PA, and MDR-PA were as follows: at 3 months, 88.96%, 88.24%, and 75.86%, respectively; at 1 year, 73.13%, 69.10%, and 51.72%; and at 3 years, 61.91%, 51.69%, and 41.10% (p = 0.034). Conclusion: MDR-PA isolation was an independent risk factor of recurrent hemoptysis post-BAE. Reducing the occurrence of MDR-PA may effectively decrease the recurrence rates of hemoptysis. [ABSTRACT FROM AUTHOR]

This research work presents the design of a multiband dual‐polarized eight‐element MIMO antenna having dimensions (0.51 λ × 0.66 λ × 0.009 λ). The antenna is observed to be radiating between 1.7 GHz and 2.4 GHz, 4.1 GHz and 10 GHz and 10.6 GHz and 19.8 GHz, featuring 3 dB axial ratio bandwidth between 9.7 and 10 GHz, 12.1 and 13.1 GHz, 14.4 and 14.5 GHz and 17.9 and 18.9 GHz. In the proposed MIMO antenna, the triangular‐shaped monopole antenna is tapered at its two sides. The isolation between the various elements of the proposed antenna is observed to be in range of −20 dB–−70 dB for the concerned frequency range. On the basis of comparison with similar existing structures, the proposed antenna demonstrates superior performance in terms of total active reflection coefficient (TARC < −5 dB), diversity gain (DG around 10 dB), channel capacity loss (CCL < 0.3 bits/s/Hz), mean effective gain (MEG) and envelope correlation coefficient (ECC < 0.024). The proposed antenna provides wide bandwidth (15.8 GHz) and high value of peak gain (6 dB). The proposed geometry is novel as it exhibits both linear as well as circular polarization with multiband response and it provides the least ECC and maximum isolation. The experimental results are found to be in agreement with the simulated results, so the proposed antenna can be useful for WLAN, C‐band, X band and Ku band applications. [ABSTRACT FROM AUTHOR]

Background: The prevalence of cognitive diseases, including Alzheimer's disease and other forms of dementia, poses a significant global health challenge due to the limited availability of effective therapeutic options. Recent years have witnessed a growing emphasis in research on the exploration of natural compounds and their derivatives as prospective therapeutic agents for cognitive impairments. Main body Xanthotoxin, a furanocoumarin compound derived from botanical sources, exhibits promising therapeutic promise in several neurological conditions such as depression, neuronal inflammation, Alzheimer's disease, vascular cognitive impairment, epilepsy, and Parkinson's disease. This potential stems from its notable neuroprotective, antioxidant, and anti-inflammatory characteristics. The present study offers a comprehensive examination of the acquisition of XAT from both natural sources and synthetic means. It delves into the significance of XAT in the treatment of cognitive disorders and delineates potential avenues for future research in the domain of XAT and cognitive disorders. Conclusion: Ongoing research and advancements in the field of XAT have the potential to enhance its use as a potent therapeutic intervention for cognitive impairments, consequently enhancing the holistic welfare of those afflicted by these incapacitating disorders. [ABSTRACT FROM AUTHOR]

This paper proposes and analyzes a 2 × 2 multi‐input multioutput antenna based on an open‐loop resonator structure and an inverted L structure. The top layer of the antenna uses symmetric inverted hook‐shaped and inverted L branches and is connected to the bottom layer of the dual‐feed port and the ground plane using the VIAs, respectively. The open‐loop resonator structure at the bottom of the antenna and the top layer of the branched circuit are resonated to provide wide‐bandwidth and dual‐frequency characteristics. The substrate uses FR4 with an area of 40 × 30 × 0.8 mm3. Although the two antenna feed ports are very close to each other, using via to connect the defective bottom ground structure and the top inverted L‐shaped branch, the isolation still achieves an excellent performance of 15 dB. The antenna has multiband application characteristics, and the bands include 2.29–5.51, 6.14–10.26, 2.29–5.33, 5.19–6.64, and 6.78–10.16 GHz. In pattern and MIMO transmission efficiency measurement, the peak radiation efficiency is 89%, the peak gain is 8.5 dBi, and the ECC is less than 0.034. In the transmission efficiency measurement mode using 64‐QAM, watching the error vector magnitude at 2.56 and 5.11 GHz, the demodulation effect is very good, and the respective throughput results are 100% and 79.2%. The broadband characteristics of the antenna have a variety of applications, and it is simulated at the specific absorption rate, which meets the standards set by the Federal Communications Commission and is very suitable for use in wearable devices. The antenna can be applied to the X‐band, n77, n79, Sub‐6 GHz, WiMax, DSRC, WiFi 6, WiFi 6E, WiFi 7, C‐V2X, and C‐band. [ABSTRACT FROM AUTHOR]

Exosomes are one type of nanosized membrane vesicles with an endocytic origin. They are secreted by almost all cell types and play diverse functional roles. It is essential for research purposes to differentiate exosomes from microvesicles and isolate them from other components in a fluid sample or cell culture medium. Exosomes are important mediators in cell–cell communication. They deliver their cargos, such as mRNA transcripts, microRNA, lipids, cytosolic and membrane proteins and enzymes, to target cells with or without physical connections between cells. They are highly heterogeneous in size, and their biological functions can vary depending on the cell type, their ability to interact with recipient cells and transport their contents, and the environment in which they are produced. This review summarized the recent progress in exosome isolation and characterization techniques. Moreover, we review the therapeutic approaches, biological functions of exosomes in disease progression, tumour metastasis regulation, immune regulation and some ongoing clinical trials. [ABSTRACT FROM AUTHOR]

Salvia dumetorum essential oil (SDEO) was obtained using a Clevenger apparatus by hydrodistillation approach. The chemical composition of the essential oil was determined by GC-MS analysis. In this study SDEO was screened for its antibacterial activity against Staphylococcus aureus, Bacillus subtilis, Escherichia coli, and Candida albicans and the amount of biofilm formed by Streptococcus mutans bacteria. For the first time the chemical composition of SDEO growing in Central Kazakhstan was established. The results obtained from GC-MS showed the predominance of sesquiterpenoids (54.15%). The antibacterial study results showed that SDEO exhibited strong antibacterial activity against B. subtilis and S. aureus in vitro and also demonstrated an inhibitory effect on S. mutans biofilm formation on 1% sucrose medium. During the study, no antibacterial activity was detected against E. coli and C. albicans. These results demonstrated that SDEO can be used in the development of new antibacterial and anti-caries therapeutic dental products. [ABSTRACT FROM AUTHOR]

Bovine rotavirus (BRV) is the main cause of acute gastroenteritis in calves, resulting in significant economic losses to the cattle industry worldwide. Additionally, BRV has multiple genotypes, which could enable cross-species transmission, thereby posing a significant risk to public health. However, there is a problem of multiple genotypes coexisting in BRV, and the cross-protection effect between different genotypes of rotavirus strains is not effective enough. Therefore, mastering clinical epidemic genotypes and using epidemic genotype strains for vaccine preparation is an effective means of preventing and controlling BRV. In this study, BRV strain DQ2020 in MA104 cells was identified by transmission electron microscopy (TEM), reverse transcription polymerase chain reaction (RT-PCR), and colloidal gold immunochromatographic test strips. The whole genome of BRV strain DQ2020 was sequenced and pathogenicity in suckling mice was assessed. The results showed that after 10 passages in MA104 cells, BRV strain DQ2020 induced cytopathic effects. Wheel-shaped virus particles (diameter, *80 nm) were observed by TEM. A target band of 382 bp was detected by RT-PCR, a positive band was detected with the colloidal gold immunochromatographic test strips, and significant green fluorescence was observed by indirect immunofluorescence (IFA). The highest median tissue culture infectious dose of strain DQ2020 after 9 passages in MA104 cells was 10-4.81 viral particles/0.1 mL. Based on phylogenetic analysis of 11 gene fragments, the genotype of BRV strain DQ2020 was G6-P[1]-I2-R2-C2-M2-A11-N2-T6-E2-H3, confirming transmission of the G6-P[1] genotype in Chinese cattle herds. Further analysis showed that the isolated strain was a reassortant of bovine (VP7, VP6, NSP3, and NSP5), human (VP4, VP1, VP2, VP3, NSP2, and NSP4), and ovine (NSP1) rotaviruses. BRV strain DQ2020 caused damage to the intestinal villi of suckling mice and diarrhea, confirming pathogenicity. In summary, this study identified a reassortant strain of bovine, human, and ovine rotavirus that is pathogenic to lactating mice, and conducted whole genome sequence analysis, providing valuable insights for the genetic evolution of the virus and the development of vaccines. [ABSTRACT FROM AUTHOR]

The present work aims to design an ultra-wideband Quad-port MIMO antenna structure with good gain and isolation for the THz spectrum to cover 6G communication applications. The size of the presented design is 130 × 130 × 1.6 µm3. The structure is designed using a polyamide material. The analysis of structure was carried out over the 0.1THz to 150THz. The optimum shape of the radiating structure is designed based on four iterations. The fractal property of the structure is provided by engraving a patch with four circles. The result is optimized by varying the fractal's radius and the ground region's length. The bandwidth enhancement and impedance improvement is achieved due to the partial ground approach. The uncomplicated form of the radiating patch facilitates the process of manufacturing. The use of the partial ground technique is effective in mitigating mutual coupling. The suggested design offers an extensive range of frequencies, spanning 96 THz (49THz to 145THz), and achieves a reflection coefficientof − 53.56 decibels. The design yields a substantial gain of 19.60 dB and achieves consistent isolation of over 30 dB. The performance of further analyses, such as radiation pattern, E-field, H-field, and diversity performance indicators, is evaluated and found to be within the allowable range. The proposed design is very appropriate for 6G communication, high-resolution medical imaging, and industrial automation. [ABSTRACT FROM AUTHOR]

The need for faster communication in today's world has led to the need for antennas with high bandwidth and gain. A novel THz MIMO antenna based on metamaterial is presented in this manuscript for high-speed communication applications with broad bandwidth and high gain. The THz MIMO metamaterial antenna is designed using a C-shaped metamaterial resonator. The metamaterial antenna is also compared with a simple square patch MIMO antenna design for different antenna parameters like gain, reflection, and transmission coefficients. The metamaterial MIMO antenna gives a higher bandwidth of around 1.95 THz, three operating bands and a maximum isolation of about 80 dB. The maximum gain of the metamaterial MIMO design is 20.4 dB. The metamaterial MIMO antenna is also optimized for its geometrical parameters like slot length, slot width, substrate thickness and ground layer thickness. The optimized value of slot length, slot width, substrate and ground layer thicknesses are obtained as 10 µm, 10 µm, 1.5 µm and 1 µm, respectively. The MIMO parameters like ECC, DG, MEG, CCL and TARC are analyzed. All these parameter values fall within the standard requirement for MIMO antennas. The designed metamaterial MIMO antenna can be a good pick for 6G/TWPAN communication devices. [ABSTRACT FROM AUTHOR]

A two‐channel high‐selectivity, wideband bandpass filter has been presented. This is based on a microstrip mixed‐impedance resonator and defected ground structure with the mixed coupling strategy. Two pairs of peripheral stepped‐impedance resonator (SIR) structures are used as stubs, achieving TZs at the immediate lower and upper cut‐off sides of the passband and control the bandwidth, while the other two transmission zeros on the upper cut‐off side are yielded through the defected ground structure, and the coupling between the SIR and uniform‐impedance resonators components, respectively. A prototype of the circuit is fabricated and measured for validation. The measured results exhibited insertion and return losses better than 1.1 and 20.5 dB, respectively, and high isolation levels exceeding 19 dB. In total, four transmission zeros are achieved. The designed filtering circuit realized a 3‐dB fractional bandwidth of 58.1%. There is a 0.95f0 stopband at the upper cutoff. The fabrication of the prototype shows good agreement between the simulated and measured performance. [ABSTRACT FROM AUTHOR]

Nanocellulose is considered a promising and sustainable biomaterial, with excellent properties of biorenewability with improved mechanical properties. As a unique natural biopolymer, it has been applied to many different industries, where efficient and environmentally friendly productions are in demand. For the first time, ferns from the class Polypodiopsida were used for the isolation of cellulose fibers, which was performed using a chemo-mechanical method. As chemical treatment plays a crucial role in the isolation of nanocellulose, it affects the efficiency of the extraction process, as well as the properties of the resulting nanocellulose. Therefore, mechanical fibrillation was performed via grinding, while the chemical process consisted of three different treatments: alkali treatment, bleaching, and acid hydrolysis. In three different experiments, each treatment was separately prolonged to investigate the differing properties of isolated nanocellulose. Structural analysis and morphological analysis were investigated by SEM, EDS, FT-IR, and DLS. The thermal stability of cellulose fibers was investigated by TGA/DSC. The morphology of obtained nanocellulose was confirmed via SEM analysis for all samples, with particles ranging from 20 nm up to 600 nm, while the most consistent sizes were observed for NC3, ranging from 20 to 60 nm. FT-IR spectra showed prominent absorption peaks corresponding to cellulose, as well as the absence of absorption peaks, corresponding to lignin and hemicellulose. The EDS confirmed the elemental purity of nanocellulose, while TGA/DSC indicated higher thermal stability of nanocellulose, compared to untreated fern, which started to degrade earlier than nanocellulose. Such characteristics with unique properties make nanocellulose a versatile biomaterial for the industrial production of cellulosic materials. [ABSTRACT FROM AUTHOR]

The orange-spotted grouper (Epinephelus coioides) is an important mariculture fish in China. However, in recent years, with the rapid development of aquaculture activities, outbreaks of viral diseases have affected the grouper aquaculture industry, causing severe economic losses. In the present study, we isolated and identified a virus from diseased, orange-spotted groupers from an aquaculture farm in Hainan Province, China. The isolated virus was identified as orange-spotted grouper iridovirus, hence named the orange-spotted grouper iridovirus Hainan strain (OSGIV-HN-2018-001). OSGIV-HN-2018-001 induces a cytopathic effect after the infection of mandarin fish (Siniperca chuatsi) brain clonal passage (SBC) cells. In addition, the cytoplasm of the OSGIV-HN-2018-001-infected SBC cells was found to contain a large number of hexagonal virus particles with a diameter of approximately 134 nm. Using the Illumina NovaSeq system, we assembled the sequence data and annotated the complete genome of OSGIV-HN-2018-001 (GenBank accession number: PP974677), which consisted of 110,699 bp and contained 122 open reading frames (ORFs). Phylogenetic tree analysis showed that OSGIV-HN-2018-001 was most closely related to ISKNV-ASB-23. The cumulative mortality rate of groupers infected with OSGIV-HN-2018-001 reached 100% on day 8. The spleens were enlarged and blackened after the dissection of the dying groupers. These results contribute to the understanding of the molecular regulatory mechanism of the iridovirus infection and provide a basis for iridovirus prevention. [ABSTRACT FROM AUTHOR]

This study aimed to identify the primary pathogenic bacteria responsible for the mortality of the Chinese giant salamander (Andriasd davidianus). A pathogenic bacterium was isolated from a diseased Chinese giant salamander exhibiting typical symptoms under aseptic conditions and then identified by morphological examinations, biochemical analysis, and the sequence analysis of bacterial 16S rRNA. Artificial infection testing was then conducted to determine the pathogenicity of the isolated bacteria strain. Drug susceptibility tests were conducted using the agar diffusion method. The isolated pathogenic bacteria, named DN-2, was successfully identified as Acinetobacter lwoffii. The artificial infection showed that the typical symptoms of the disease could be replicated as the ones originally occurred, and this bacterium exhibited high pathogenicity to the Chinese giant salamander. In the Chinese giant salamander, the median lethal dosage (LD50) of A. lwoffii DN-2 for A. davidianus was determined to be 4.63*104 CFU/g. Drug sensitivity testing showed that these bacteria were highly sensitive to erythromycin, gentamicin, neomycin, streptomycin, midecamycin, ciprofloxacin, piperacillin, florfenicol, doxycycline, carbenicillin, and sulfanilamide. In summary, A. lwoffii was identified as the primary pathogen responsible for the demise of A. davidianus. Our study first presents how these bacteria harm Chinese giant salamanders. [ABSTRACT FROM AUTHOR]

Identifying and analysing isolated populations is critical for conservation. Isolation can make populations vulnerable to local extinction due to increased genetic drift and inbreeding, both of which should leave imprints of decreased genome‐wide heterozygosity. While decreases in heterozygosity among populations are frequently investigated, fewer studies have analysed how heterozygosity varies among individuals, including whether heterozygosity varies geographically along lines of discrete population structure or with continuous patterns analogous to isolation by distance. Here we explore geographical patterns of differentiation and individual heterozygosity in the threatened eastern barred bandicoot (Perameles gunnii) in Tasmania, Australia, using genomic data from 85 samples collected between 2008 and 2011. Our analyses identified two isolated demes undergoing significant genetic drift, and several areas of fine‐scale differentiation across Tasmania. We observed discrete genetic structures across geographical barriers and continuous patterns of isolation by distance, with little evidence of recent or historical migration. Using a recently developed analytical pipeline for estimating autosomal heterozygosity, we found individual heterozygosities varied within demes by up to a factor of two, and demes with low‐heterozygosity individuals also still contained those with high heterozygosity. Spatial interpolation of heterozygosity scores clarified these patterns and identified the isolated Tasman Peninsula as a location where low‐heterozygosity individuals were more common than elsewhere. Our results provide novel insights into the relationship between isolation‐driven genetic structure and local heterozygosity patterns. These may help improve translocation efforts, by identifying populations in need of assistance, and by providing an individualised metric for identifying source animals for translocation. [ABSTRACT FROM AUTHOR]

Islet transplantation represents a promising therapeutic approach for diabetes management, yet the isolation and evaluation of pancreatic islets remain challenging. This study focuses on the isolation of islets from rabbit pancreases, followed by a comprehensive assessment of their viability and functionality. We developed a novel method for isolating islet cells from the pancreas of adult rabbits. We successfully isolated viable islets, which were subsequently evaluated through a combination of viability assays, an insulin enzyme-linked immunosorbent assay (ELISA), and fluorescence lifetime imaging microscopy (FLIM). The viability assays indicated a high percentage of intact islets post-isolation, while the insulin ELISA demonstrated robust insulin secretion in response to glucose stimulation. FLIM provided insights into the metabolic state of the islets, revealing distinct fluorescence lifetime signatures correlating with functional viability. Our findings underscore the potential of rabbit islets as a model for studying islet biology and diabetes therapy, highlighting the efficacy of combining traditional assays with advanced imaging techniques for comprehensive functional assessments. This research contributes to the optimization of islet isolation protocols and enhances our understanding of islet functional activity dynamics in preclinical settings. [ABSTRACT FROM AUTHOR]

Ezetimibe (EZE) is a dyslipidemic agent used to treat hyperlipidemia along with statins and diet changes. To ascertain the drug's degradation profile, stress tests were conducted on it in accordance with International Council for Harmonization recommendations. An ultrahigh‐performance liquid chromatography–mass spectrometry method was developed and validated for the identification and quantification of EZE and its degradants. Using preparative high‐performance liquid chromatography, all impurities were isolated, and their structures were characterized thoroughly using advanced spectral techniques. The drug was relatively stable in basic, peroxide, photolytic, and thermal conditions; however, five degradants were observed in acid degradation. Among the five degradants, three degradation products eluted as Peak‐1, Peak‐2, and Peak‐3 which were found to be novel impurities that were not reported previously. However, the remaining two impurities were identified as Peak‐4 and Peak‐5, despite the insufficient information available. The present study has focused on the isolation of these five acid degradation products and the structural confirmation of these degradants by highly efficient spectral techniques. Moreover, the possible degradation mechanism of the azetidinone ring in the presence of acid has been explained, which might be helpful in determining the quality and purity of EZE and similar pharmaceutical compounds. [ABSTRACT FROM AUTHOR]

Summary: In this study, ultrasound‐assisted covalent binding method, free radical‐induced method, was used to prepare Moringa protein isolate (MPI) – gallic acid conjugates (MPI‐GAC) to examine the improvement of the sonication application. Additionally, the influence of polyphenol conjugation on functional and structural attributes of MPI was assessed thru spectral analysis, scanning electron microscopy, antioxidant activity, emulsification, foaming, and water and oil holding properties. The enhanced value of grafted polyphenol species was observed after introducing ultrasound treatment during free radical mediation process. The structural changes in protein by gallic acid conjugation were confirmed by using FT‐IR which illustrated the modification in MPI‐GAC with modified peak width of the amide‐I band. Scanning electron microscopy also showed a significant difference in the structure of MPI and MPI‐GAC. The grafting of gallic acid onto MPI directed under ultrasound‐assisted conditions conferred distinctive functional characteristics, containing elevated emulsification capacity (51.4 mg mL−1) along with enhanced water and oil holding capacity by 2.07 and 5.11 g g−1, respectively. Furthermore, conjugation also increased the protein solubility 89.3–97.6% and significant trend (68.04–88.06%) of antioxidant capacity was observed by increasing the concentration. The findings of this research work offer new insights on the effective fabrication of MPI‐GAC with multifaceted functionalities, thus broadening the scope and possibilities of high‐value Moringa seed protein. [ABSTRACT FROM AUTHOR]

Bio-orthogonal labeling of vesicles conjugated with fluorescent molecular groups, enables in vivo bioimaging to monitor their behavior, thus aiding in diagnostics or revealing the internalization mechanisms of extracellular vesicles (EVs). Orthogonally based magnetic beads, microfluidics, and microarray chips specifically and in a high-throughput manner isolate EVs derived from various cancers for diagnosis of diseases and cancer development. By loading drugs onto the surface of EVs or forming covalent bonds between EVs and cells through bio-orthogonal methods, the therapeutic efficiency of EVs can be enhanced. Bio-orthogonal glycometabolism enables EVs to directly target tumor cells and their derived EVs in vivo , thereby inhibiting tumor metastasis and growth. Extracellular vesicle (EV)-based therapeutics have gained substantial interest in the areas of drug delivery, immunotherapy, and regenerative medicine. However, the clinical translation of EVs has been slowed due to limited yields and functional heterogeneity, as well as inadequate targeting. Engineering EVs to modify their inherent function and endow them with additional functions has the potential to advance the clinical translation of EV applications. Bio-orthogonal click chemistry is an engineering approach that modifies EVs in a controlled, specific, and targeted way without compromising their intrinsic structure. Here, we provide an overview of bio-orthogonal labeling approaches involved in EV engineering. We also present the isolation methods of bio-orthogonally labeled vesicles using magnetic beads, microfluidics, and microarray chip technologies. We highlight the in vivo applications of bio-orthogonal labeling EVs for diagnosis and therapy, especially the exciting potential of bio-orthogonal glycometabolic engineered EVs for targeted therapies. [ABSTRACT FROM AUTHOR]

Social isolation can have long-term effects on brain development and behavior and increases the risk of developing clinical conditions, including anxiety disorders. One modulator of the stress response is gamma-aminobutyric acid, an inhibitory neurotransmitter synthesized by glutamic acid decarboxylase (GAD). This study examined sex differences in behavior and GAD expression following prolonged social isolation beginning in adolescence in Long Evans rats. Males and females were equally divided into group-housed (GH) and socially isolated conditions on Postnatal Day 28 (n = 8 per group). Beginning 5 weeks later, tests were conducted for anxietylike behaviors (open-field test and elevated plus maze), social interactions (sociability test), and spatial memory (novel object location). Sex differences in behavior were observed, with GH females showing fewer anxietylike behaviors in the open-field test and elevated plus maze and spending more time with objects (sociability task) compared to GH males. Isolation had no effect on males but increased anxiety and reduced neophilic measures in females, removing sex differences. On the sociability task, all groups spent more time with novel rats compared to objects, suggesting social interest was retained after isolation. In the hippocampus, isolation reduced GAD in both sexes, and sex differences were seen (F > M). However, no group differences in behavior were observed in the hippocampal-dependent novel object location task. Our findings suggest that prolonged social isolation beginning in adolescence is anxiogenic for female Long Evans rats. Furthermore, sex and housing impact hippocampal GABA-ergic activity, which may have important implications in the treatment of anxiety disorders. [ABSTRACT FROM AUTHOR]

Alfred, Lord Tennyson's narrative poem The Lady of Shalott explores profound themes of isolation, artistry, and the quest for freedom against the backdrop of Victorian societal norms. Tennyson, renowned for his lyrical language and introspective style, crafts a haunting tale of a cursed woman confined to a tower on the island of Shalott. Forbidden to directly observe the outside world, the Lady weaves scenes reflected in a mirror, navigating between artistic expression and the stifling constraints of her existence. This article delves into Tennyson's portrayal of the Lady's isolation, emphasizing how her imprisonment symbolizes not only physical confinement but also psychological and emotional barriers. Through meticulous analysis of Tennyson's imagery, narrative techniques, and thematic exploration, the article examines the Lady's tragic journey towards self-discovery and transcendence, ultimately revealing broader insights into Victorian notions of femininity, the power of art, and the perennial human desire for autonomy. [ABSTRACT FROM AUTHOR]

Calophyllum species are well known due to their abundance of potentially beneficial phytochemicals, such as xanthones, coumarins, and others. However, Calophyllum gracilentum is an understudied specie with very limited information. Medicinal plants have been proven to have therapeutic potential in managing neurological disorders associated with acetylcholinesterase (AChE) dysregulation. Still, there has been little investigation on Calophyllum plants for this purpose. Herein, we report on the isolation of a new oxygenated xanthone 5,10-dihydroxy-9-methoxy-2,2-dimethyl-12-(3-methylbut-2-enyl) pyrano[3,2- b ] xanthen-6(2 H)-one (1) and eleven known xanthones (2–12), three chromanone acids (13–15), and phytosterols (16–18), respectively from the stem bark of the Calophyllum gracilentum. The evaluation of AChE inhibitory activity showed that all the extracts and xanthones (2 , 5 , 6 , 8 , 9 , 12) tested have potential AChE inhibitory activity. Compounds (5) and (12) are prospective AChE inhibitors with IC 50 values of 1.8 ± 0.0001 and 4.0 ± 0.0002 µmol/L. The molecular docking analysis demonstrated compound (5) and compound (12) bind well to the active site which is the anionic site containing Trp 84 and Asp 72 in the of Torpedo californica acetylcholinesterase (TcAChE) through π-π stacking, hydrogen bonding, and π-donor hydrogen bond from the xanthone ring, besides π-alkyl and π-σ interactions from the substituent group with the binding energy of −11.1 kcal/mol for compound (5) and binding energy of −10.4 kcal/mol for compound (12). [Display omitted] • A new xanthone, 12 known xanthones, chromanone acids and phytosterols were isolated. • 5 and 12 showed strong inhibition of AChE activity. • 5 and 12 bound to the AChE active site by π-π stacking. [ABSTRACT FROM AUTHOR]

Background: Leadership can be an isolating experience and leaders from underrepresented groups (URGs) may experience even greater isolation and vulnerability because of lack of representation. Given the collaborative nature of medicine, leadership programs for physicians need to address isolation. Social support is one mechanism to combat this isolation; however, most leadership programs focus exclusively on skills building. Purpose: The Stanford Network for Advancement and Promotion (SNAP) program was developed to reduce isolation among physician leaders from URGs in academic medicine leadership by building a supportive network of peers. Methodology/Approach: Ten women physicians from diverse racial/ethnic backgrounds were invited to participate in SNAP. Annual surveys were administered to participants to assess the effectiveness of SNAP on decreasing feelings of isolation and increasing professional leadership growth. The authors charted the expansion and adaptation of the program model across gender and in additional settings. Results: SNAP effectively created a sense of community among the physician leaders. Participants also reported feeling challenged by the program and that they had grown in terms of critical thinking, organizational knowledge, and empowerment as leaders. Participants found community building to be the most valuable program component. Because of this success, the SNAP model has been adapted to create 10 additional cohorts. Conclusion: Leadership programs like SNAP that focus on reducing isolation are instrumental for retaining and promoting the career advancement of physicians from URGs. Practice Implications: Developing a diverse workforce of academic physicians is essential to providing high-quality and equitable clinical care, research, and medical education. [ABSTRACT FROM AUTHOR]

A Multiple Inputs Multiple Outputs (MIMO) antenna with a compact dimension of 0.28λ0 0.36λ0 for ultra-wideband (UWB) spectrum applications has been proposed, where, λ0 is the free space wavelength at the resonating frequency of 3.34 GHz. The proposed work consists of two identical radiating antenna elements and a modified T-shaped ground structure with a loaded rectangular slot. The modified T-shaped structure greatly affects impedance matching and electromagnetic field distribution. Due to that, the impedance bandwidth, isolation, compactness, and other MIMO parameters are also enhanced. The proposed antenna confirms a measured UWB frequency of 3.0 - 11.66 GHz. The antenna isolation greater than 20 dB throughout the working spectrum is achieved. The results further signify that the antenna displays good MIMO performance with respect to envelope correlation coefficients (ECC), diversity gain (DG), channel capacity loss (CCL), and gain. The ECC value of less than 0.01, diversity gain above 9.98 dBi, peak gain of 3.52 dBi, and CCL lesser than 0.25 bits/S/Hz are achieved. The group delay and phase response are also studied for ensuring distortion-less signal transmission under broad impedance bandwidth. [ABSTRACT FROM AUTHOR]

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Achieving a compact-size, high-performance antenna covering the K-Ka bands is not an easy task. A Multi-Input Multi-Ooutput (MIMO) funnel-shaped wideband antenna for RADAR applications is introduced in this paper. One of the flaws of the MIMO antenna is its size. In modern communications, the space given for an antenna is minimal due to the compact size of the communication devices. In the present work, by keeping space occupancy in mind, two element compact antennas, with 76x34x1.6 mm3 size, operating bandwidth of 24.7 GHz, isolation greater than 18.9 dB, and gain of 9.26 dB, were designed. The designed funnel shape patches and defective ground structure provide better gain and isolation, therefore allowing for larger bandwidth. Furthermore, the simulated and measured results are in good agreement. [ABSTRACT FROM AUTHOR]

The search for effective treatments for Autism Spectrum Disorder (ASD) has led to the investigation of herbal remedies, some of which show promise in addressing the complex array of symptoms associated with the disorder. This study presents a comprehensive examination of a specific herbal drug, focusing on the isolation and characterization of its active compounds, as well as its potential ASD-related activity. The isolation process involved the extraction of bioactive constituents from the herbal drug using state-of-the-art techniques such as chromatography and spectroscopy. The isolated compounds were then meticulously characterized to determine their chemical structures and purity, providing essential insights into their pharmacological properties. Subsequently, the study delves into the ASD activity evaluation of the herbal drug's constituents. In vitro experiments assessed their interaction with neuroreceptors and their impact on neuronal signaling pathways. In vivo studies, employing animal models and behavioral assessments, explored the potential therapeutic effects of the herbal drug on ASD-related behaviors, neurobiology and cognitive functions. The findings of this research contribute to our understanding of the mechanisms underlying herbal interventions for ASD and may offer new avenues for the development of targeted treatments. Ultimately, the study bridges the gap between traditional herbal medicine and modern scientific approaches, paving the way for potential therapeutic solutions in the challenging landscape of ASD management. [ABSTRACT FROM AUTHOR]

Duck hepatitis A virus (DHA) is very fatal viral disease affecting young ducklings under one month old. The disease is generally spread among duckling flocks inducing sever dramatic and economic losses. The present research highlights investigation of duck hepatitis virus through collection of hundred spleen and liver field samples from various commercial 3-11 days old duckling sectors (Pekin and Mullard) at ten Egyptian governorates in 2022 and 2023 with historical view of high mortalities and nervous manifestations with background of previous immunization. The clinically infected specimens were directly screened using RT-PCR assay to detect duck hepatitis A virus through amplification of VP1 gene that reveals only one sample (obtained from Menofia governorate) was positive for DHAV-3. BLAST analysis of Partial obtained sequence of VP1 gene showed that it was closely related Egyptian strain (accession number OR543968) besides nucleotides and amino acid changes were observed in comparison with other strains. Phylogenetic analysis of the obtained strain revealed clustering with viruses of Chinese origin and distinctive from vaccinal strains utilized in Egypt. Successful isolation of duck hepatitis A virus was achieved through inoculation of tissue homogenates into allantoic cavity of 9-11 day old embryonated chicken eggs. The outcomes of this work supplied rationalized knowledge about the epidemiological criteria of DHA virus in Egypt; emphasize the significance of DHA survey and vaccine selection. [ABSTRACT FROM AUTHOR]

Bioactive flavonoid epicatechin has been reported in the peel of litchi fruit but isolated from its hydroalcoholic extracts. This study isolated epicatechin with cellular glucose uptake modulatory and ROS production inhibitory properties from the ethyl acetate (EtOAc) extract using a bioassay-guided approach. The fruit peel was defatted with hexane and sequentially extracted using dichloromethane (DCM), EtOAc, methanol (MeOH) and water. In vitro phytochemical models, namely antioxidant (Fe3+ reducing, radical scavenging and anti-linoleic acid peroxidative) and glycaemic control (α-glucosidase and α-amylase inhibitory and glucose uptake modulatory), were employed for the bioassay-guided isolation, while the isolated compound was characterised using NMR and mass spectrometry and assessed for dose-dependent inhibition of α-glucosidase and lipopolysaccharide (LPS)-induced cellular ROS production, as well as modulation of cellular glucose uptake. Relative to the other extracts, the EtOAc extract had appreciable phenol and flavonoid contents, which perhaps influenced its potent anti-lipid peroxidative (65.0%) and α-glucosidase inhibitory (52.4%) effects. The α-glucosidase inhibitory potency of the fractions (1–8) from the EtOAc extracts correlated with their flavonoid contents, with fraction 5 outperforming other fractions. The fraction comprised a pool of fractions obtained from the DCM:MeOH:water (7:3:0.281 v/v/v) solvent system. LC-MS revealed the predominant presence of epicatechin in fraction 5, which was later isolated from one of the sub-fractions (sub-fraction 4) of fraction 5. This sub-fraction had stronger anti-lipid peroxidative (65.5%), α-glucosidase inhibitory (65.8%) and glucose uptake modulatory (38.2%) effects than the other sub-fractions from fraction 5, which could have been influenced by the isolated epicatechin. Moreover, the isolated epicatechin inhibited α-glucosidase (IC50 = 35.3 µM), modulated cellular glucose uptake (EC50 = 78.5 µM) and inhibited LPS-induced ROS production in RAW 264.7 macrophages in a dose-dependent fashion [IC50 = 18.9 µM; statistically comparable (p > 0.05) to ascorbic acid, IC50 = 9.57 µM]. Epicatechin from litchi peel EtOAc extract could potentiate glucose uptake modulatory, α-glucosidase inhibitory and ROS suppressive capacities, which could be influential in the use of litchi fruit peel for managing diabetes and associated oxidative damage. [ABSTRACT FROM AUTHOR]

Modern wireless communication systems have undergone a radical change with the introduction of multiple-input multiple-output (MIMO) antennas, which provide increased channel capacity, fast data rates, and secure connections. To achieve real-time requirements, such antenna technology needs to have good gains, wider bandwidths, satisfactory radiation characteristics, and high isolation. This article presents an eight-element CPW-fed antenna for the 5G mid-band. The proposed antenna consists of eight symmetrical, modified circular monopole antennas with a connected CPW-fed ground plane that offers 24 dB isolation over the operating range. The antenna is further investigated in terms of the scattering parameters, and radiation characteristics under both the x and y-axis bending scenarios. The antenna holds a volume of 83 × 129 × 0.1 mm3 and covers a measured impedance bandwidth of 4.5–5.5 GHz (20%) with an average gain of 4 dBi throughout the operating band. MIMO diversity performance of the antenna is performed, and the antenna exhibits good performance suitable for MIMO applications. Furthermore, the channel capacity (CC) is estimated, and the antenna gives a value of 41.8–42.6 bps/Hz within the operating bandwidth, which is very close to an ideal 8 × 8 MIMO system. The antenna shows an excellent match between the simulated and measured findings. [ABSTRACT FROM AUTHOR]

True morels (Morchella spp.) are highly valuable and medicinal mushrooms. Saprophytic morels have been cultivated, especially in China and some Western countries, over the last few decades. Türkiye has a rich potential in terms of wild morel diversity, with nearly 40 Morchella species in its genetic pool, though only 22 of these have been identified molecularly. It has high economic value worldwide, and Türkiye exports morels worth approximately 2 million $ annually. There is also significant interest in morel mushroom cultivation in Türkiye. In this study, 40 Morchella strains were collected and isolated from different regions of Türkiye and analyzed based on the Internal Transcribed Spacer (ITS) of the nuclear ribosomal DNA (rDNA) region. A phylogenetic dendrogram was drawn. The isolates of M. importuna, M. exima, M. exuberans, M. dunali, M. tridentina, M. crassipes, and M. esculenta were identified based on the ITS rDNA region. However, the identification of isolates 849-Kg027 and 966-Kg142 could not be determined clearly, and the isolates of M. vulgarius and M. spongiola were not distinct based on the ITS analysis. The macro-morphological features of the mycelia were investigated. Mycelia colors ranged from off-white to pale gray in the juvenile stage, orange to pale brown during early pigmentation, and pale brown to dark brown in the senescence stage. M. crassipes, M. exuberans, and 966-Kg142 formed lighter-colored mycelia, whereas M. dunali and M. vulgarius exhibited the darkest mycelial pigments. Sclerotia formation was compact, pale yellow to yellow, and abundant. In conclusion, molecular identification of Turkish morel cultures was performed, and cultural characteristics along with morphological differences were examined. The cultures have been deposited for further study in the Mushroom Gene Bank at the Atatürk Central Horticultural Research Institute. [ABSTRACT FROM AUTHOR]