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BACKGROUND: The use of DNA metabarcoding has become an increasingly popular technique to infer feeding relationships in polyphagous herbivores and predators. Understanding host plant preference of native and invasive herbivore insects can be helpful in establishing effective integrated pest management (IPM) strategies. The invasive Halyomorpha halys and native Pentatoma rufipes are piercing‐sucking stink bug pests that are known to cause economic damage in commercial fruit orchards. RESULTS: In this study, we performed molecular gut content analysis (MGCA) on field‐collected specimens of these two herbivorous pentatomids using next‐generation amplicon sequencing (NGAS) of the internal transcribed spacer 2 (ITS2) barcode region. Additionally, a laboratory experiment was set up where H. halys was switched from a mixed diet to a monotypic diet, allowing us to determine the detectability of the initial diet in a time series of ≤3 days after the diet switch. We detected 68 unique plant species from 54 genera in the diet of two stink bug species, with fewer genera found per sample and a smaller diet breadth for P. rufipes than for H. halys. Both stink bug species generally prefer deciduous trees over gymnosperms and herbaceous plants. Landscape type significantly impacted the observed genera in the diet of both stink bug species, whereas season only had a significant effect on the diet of H. halys. CONCLUSION: This study provides further insights into the dietary composition of two polyphagous pentatomid pests and illustrates that metabarcoding can deliver a relevant species‐level resolution of host plant preference. © 2024 Society of Chemical Industry. [ABSTRACT FROM AUTHOR]

Background: Interactions between alien plants and insect herbivores in introduced ranges may determine their invasion success. However, few studies have investigated whether alien plants respond differently to native and introduced herbivores in their introduced ranges and whether genotypes of alien plants matter. We conducted a greenhouse experiment to examine the effects of herbivory by a native insect (Spodoptera litura), by an introduced insect (S. frugiperda), and simultaneously by both insect species on growth, morphology, and biomass allocation of 17 genotypes of an invasive alien clonal plant Hydrocotyle verticillata, and used selection gradient analysis to test which herbivory conditions favor selection of a specific leaf or root trait value. Results: Different genotypes of H. verticillata showed significant variation in growth, morphology, and biomass allocation, but their responses to herbivory were relatively consistent. All three herbivory treatments significantly decreased total mass and stolon mass, but herbivory of S. frugiperda increased specific leaf area. Herbivory of S. litura and simultaneous herbivory of both insect species also decreased leaf mass, petiole mass, root mass, and ramet mass. Selection gradient analysis showed that leaf and root traits varied under different herbivory treatments. To achieve greater fitness, as measured by total mass and/or number of ramets, H. verticillata favored larger leaf area under herbivory by S. frugiperda, larger leaf area and lower specific leaf area under herbivory by S. litura, and larger leaf area, lower specific leaf area, and lower root-to-shoot ratio under simultaneous herbivory. Conclusions: H. verticillata demonstrated contrasting responses to herbivory by native and introduced insects, showing a remarkable ability to coordinate leaf trait plasticity and optimize biomass allocation. This strategy allows H. verticillata to achieve greater fitness under various herbivory conditions, potentially contributing to its invasion success. These findings highlight the importance of plant–herbivore interactions in shaping invasion dynamics and underscore the complex adaptive mechanism that enables invasive plants to establish and spread in introduced ranges. [ABSTRACT FROM AUTHOR]

Hemocytes are pivotal in the immune response of insects against invasive pathogens. However, our knowledge of hemocyte types and their specific function in Tribolium castaneum, an increasingly important Coleoptera model insect in various research fields, remains limited. Presently, a combination of morphological criteria and dye‐staining properties were used to characterize hemocyte types from T. castaneum larvae, and 4 distinct types were identified: granulocytes, oenocytoids, plasmatocytes and prohemocytes. Following different immune challenges, the total hemocyte counts declined rapidly in the initial phase (at 2 h), then increased over time (at 4 and 6 h) and eventually returned to the naive state by 24 h post‐injection. Notably, the morphology of granulocytes underwent dramatic changes, characterized by an expansion of the surface area and an increased production of pseudopods, and with the number of granulocytes rising significantly through mitotic division. Granulocytes and plasmatocytes, the main hemocyte types in T. castaneum larvae, can phagocytose bacteria or latex beads injected into the larval hemolymph in vivo. Furthermore, these hemocytes participate in the encapsulation and melanization processes in vitro, forming capsules to encapsulate and melanize nickel–nitrilotriacetic acid (Ni–NTA) beads. This study provides the first comprehensive characterization of circulating hemocytes in T. castaneum larvae, offering valuable insights into cell‐mediated immunity in response to bacterial infection and the injection of latex beads. These results deepen our understanding of the cellular response mechanisms in T. castaneum larvae and lay a solid foundation for subsequent investigations of the involvement of T. castaneum hemocytes in combating pathogens. [ABSTRACT FROM AUTHOR]

ABSTRACT Aim Location Time Period Major Taxa Studied Methods Results Conclusions The global, human‐mediated dispersal of invasive insects is a major driver of ecosystem change, biodiversity loss, crop damage and other effects. Trade flows and invasive species propagule pressure are correlated, and their relationship is essential for predicting and managing future invasions. Invaders do not disperse exclusively from the species' native range. Instead, the bridgehead effect, where established, non‐native populations act as secondary sources of propagule, is recognised as a major driver of global invasion. The resulting pattern of global spread arises from a mixture of global interactions between invasive species, their vectors and, their invaded ranges, which has yet to be fully characterised.Global.1997–2020.Insects.We analysed 319,283 border interception records of 514 insect species from a broad range of taxa from four national‐level phytosanitary organisations. We classified interceptions as coming from species native range or from bridgehead countries and examined taxonomic autocorrelation of global movement patterns between species.While 65% of interceptions originated from bridgehead countries, highlighting the importance of the bridgehead effect across taxa, patterns among individual species were highly variable and taxonomically correlated. Forty per cent of species originated almost exclusively from their native range, 28% almost exclusively from their non‐native range and 32% from a mix of source locations. These patterns of global dispersal were geographically widespread, temporally consistent, and taxonomically correlated.Dispersal exclusively from bridgeheads represents an unrecognised pattern of global insect movement; these patterns emphasise the importance of the bridgehead effect and suggest that bridgeheads provide unique local conditions that allow invaders to proliferate differently than in their native range. We connect these patterns of global dispersal to the conditions during the human driven global dispersal of insects and provide recommendations for modellers and policymakers wishing to control the spread of future invasions. [ABSTRACT FROM AUTHOR]

Herbivorous insects generally choose host plants that optimize their growth. However, many specialists choose chemically defended host plants that slow their growth, prioritizing protection from natural enemies.Invasive specialist herbivores may have an added advantage in their non‐native range where they lack co‐evolved specialist natural enemies, in addition to being unpalatable to generalist predators.Here, we explore the diet preferences of the invasive yellowmargined leaf beetle, Microtheca ochroloma and those of native natural enemies. We measured M. ochroloma preferences among six Brassica cultivars varying in glucosinolate concentrations.In both field and laboratory experiments, M. ochroloma preferred the relatively less defended host plants, mizuna and Chinese cabbage. M. ochroloma grew quickest on mizuna.Generalist predators also preferred to eat M. ochroloma larvae that had been fed mizuna in choice trials, yet in the field, predatory insects were least commonly found on mizuna, and most commonly found on plants dominated by native herbivores.M. ochroloma consumes plants that increase their susceptibility to predators, but predation pressure in the field appears to be generally low. Our results suggest that invasive specialist herbivores may prioritize growth over protection against natural enemies.Weak top‐down regulation of specialist herbivore invaders might enable them to exploit less‐defended host plants in their novel range that facilitate faster growth and exacerbate crop damage. Invasive pests may be more effectively managed by leveraging plant preferences in the novel host range in trap crop systems. [ABSTRACT FROM AUTHOR]

Examining the host range of emerging invasive insects is essential to assess their invasion potential and to anticipate the negative impacts of their spread. The ongoing North American invasion of spotted lanternfly (SLF) [ Lycorma delicatula (White, 1845)] threatens agricultural, urban, and natural areas. The survival and development of SLF nymphs on Washington navel orange [ Citrus sinensis (L.) Osbeck (Sapindales: Rutaceae)] trees were assessed in a quarantine facility. Results indicated that SLF nymphs can develop to at least the third instar by feeding exclusively on Washington navel orange. This finding suggests that, at least up to the third stage of nymphal development, Washington navel orange might be a suitable host for SLF, highlighting the possibility that this invasive pest represents an unrecognized threat to this globally important crop and possibly to other Citrus species. [ABSTRACT FROM AUTHOR]

Sterile insect technique (SIT) is a useful strategy for preventing and mitigative establishment of invasive insect species. SIT of the pest tephritid Mediterranean fruit fly, Ceratitis capitata (Wiedemann, 1824) WiedemannWiedemann , has been effective in preventing population establishment in vulnerable agricultural areas of the United States. However, irradiation-induced sterilization can have detrimental impacts resulting in reduced performance metrics. Mediterranean fruit fly males reared for SIT have been shown to have differences in their microbiomes relative to other population sources, which has been postulated to be a factor in how well flies compete with wild conspecifics. To identify baseline performance metrics on the effects of irradiation on the gut microbiome of mass-reared flies in Hawai'i, a study was performed to assess performance metrics and microbiome (bacterial 16S rRNA) variation across multiple timepoints. Mediterranean fruit fly pupae were selected from mass-reared trays intended for release, and paired samples were either irradiated or remained as controls and transported to the laboratory for evaluation. Irradiated flies exhibited fewer successful fliers, more rapid mortality rates, and were less active relative to control nonirradiated flies. Contrary to initial expectations, irradiation did not exert substantial impacts on the composition or diversity of bacterial reads. Samples were primarily comprised of sequences classified as Klebsiella and there were low levels of both read and taxonomic diversity relative to other 16S surveys of medfly. Although this study does not demonstrate a strong effect of irradiation alone on the Mediterranean fruit fly microbiome, there are several explanations for this discrepancy. [ABSTRACT FROM AUTHOR]

Urban environments are vulnerable to the introduction of non-native species and sometimes contribute to their invasion success. Knowing how urban landscape features affect the population dynamics of exotic species is therefore essential to understand and manage these species. The spotted-wing drosophila, Drosophila suzukii, is a highly polyphagous fruit fly that has become a very problematic invasive species over the last decade. Because of its important damage on fruit production, D. suzukii populations have mainly been studied in agricultural areas, while their dynamics in urban landscape remain poorly explored. The objective of this study was to investigate the role of urban environment in the invasion success of D. suzukii by identifying local and landscape factors driving the abundance of the fly along seasons and urbanization gradients. To achieve this, 526 insect traps were randomly set in four different habitats (urban forest, park, riverside and town centre) along an urbanization gradient in the city of Amiens (France), between September 2018 and August 2019. The influence of landscape and local environmental variables on Drosophilidae species diversity and composition was examined using GLM and multivariate analyses. We found that Drosophilidae species richness and abundance were negatively impacted by urbanization. The Drosophilidae community was dominated by D. subobscura and D. suzukii, but their relative abundance varied with seasons. Drosophila suzukii used urban forest during winter and also during heat waves in summer. The fly was still active in this habitat in winter when the ground was covered with snow. The cover of brambles, shrubs, soil litter and dead wood debris were identified as valuable ecological indicators of the presence of D. suzukii. We highlight the role of the different components of urban environment in the ecology of D. suzukii, particularly with regard to its winter survival. These results could serve for designing management strategies in urban habitats in order to reduce the invasion success of D. suzukii. [ABSTRACT FROM AUTHOR]

The effect of different urbanization levels on terrestrial biodiversity associated with riparian zones remains poorly studied, despite the important ecosystem services it provides in cities. Studies focused on aquatic ecosystems in urban streams reported decreases in ecological quality and species richness, and lack of sensitive taxa. Thus, we investigated the impact of urbanization on riparian zone flora and terrestrial invertebrates using as case studies nine urban streams spanning an urbanization gradient in the Portuguese city of Coimbra. An unexpectedly high number of taxa were identified (163 plants, 80 terrestrial invertebrates), 80% new registrations for the region and one endemic-rare plant. Yet, diversity varied across streams, β-diversity reaching 39% Bray-Curtis similarity and averaging 25%, due to many underrepresented terrestrial taxa (N = 62 observed only in one site). Variation across the urbanization gradient reached 59% in plants and 82% in terrestrial invertebrates. High numbers of non-native taxa (13%; N = 32), mostly plants, suggested urbanization factors, e.g. human interference and discharge variation, may have favored invasions. Indeed, constructed natural spaces along streams, like parks, supported less biodiversity. Moreover, plant richness, especially of riparian plants, was correlated negatively with percentages of surrounding impervious areas and positively with water quality. This shows urbanization has negative effects on riparian vegetation, and water quality impacts both aquatic and terrestrial communities. Our results stress urban streams as novel ecosystems constituted by high numbers of non-native taxa, and the significance of riparian zones for biodiversity preservation. Less intense intervention on riparian vegetation is recommended to increase biodiversity. [ABSTRACT FROM AUTHOR]

Tomato is a widely cultivated crop significant for its economic and nutritional benefits. The South American tomato pinworm, Tuta absoluta, originated in Peru South America and has invaded many nations, causing up to 100% yield loss in tomatoes. The pest was classified as a quarantine pest by the European Plant Protection Organization, before invading the Spain region. Later, this quarantine pest also invaded other regions of Europe, Africa and Asian countries. Invasive insect pests cause global economic losses of 70 billion dollars annually. Among the several management measures suggested against pests, insecticides are the primary method in practice among growers due to significant results, easier operations, and other crucial advantages. Anyhow, repeatedapplication of insecticides has caused the pest to evolve resistance against most of the insecticides in vogue, resulting in a chain of events like management failures, using increased doses of insecticides, intensified chemical residues in the food chain, and irreparable environmental contamination. Major insecticides globally used to control T. absoluta belong to organophosphates, synthetic pyrethroids, neonicotinoids, diamides, avermectins, spinosyns, and oxadizines. Understanding the baseline susceptibility of pests to insecticides helps for better pest management options and is the same for T. absoluta populations to insecticides. The current review paper discusses the T. absoluta distribution, biology, spread, host range, baseline insecticide susceptibility, global insecticide resistance status, and possible management inputs based on our understanding of insecticide susceptibility. The pest can be managed with integrated insecticide resistance management including molecular approaches. [ABSTRACT FROM AUTHOR]

Invasive alien insects have the potential to pose a significant threat to global agriculture, with their distinctive traits enabling rapid reproduction, successful adaptation to new environments and high distribution capability. These pests can devastate crops, livestock, biodiversity and ecosystem functioning, resulting in ecological damage and substantial economic losses. Climate change plays a crucial role in driving the invasion of these pests, creating favorable conditions for their development, and negatively impacting global biodiversity. Among invasive alien insects, fall armyworm (FAW) (Spodoptera frugiperda) (JE Smith) (Lepidoptera: Noctuidae) has emerged as a major pest species, causing significant yield losses in maize cropping outside his native range. Initially, reliance on pesticides for control proved ineffective and led to pesticide resistance. Significant progress has been made in implementing integrated pest management (IPM) strategies that integrate agro-ecological and biological approaches. This review article focuses on the compilation of IPM methods, combining agro-ecological practices and biological control agents such as parasitoids and viruses, for the effective management of FAW. Approaches such as intercropping, agronomic practices, and the use of parasitoids and viruses have shown promising results in controlling FAW. This review article provides insights into successful management methods, recommendations and suggestions for the sustainable control of FAW using agro-ecological practices, biological control agents or their combination. [ABSTRACT FROM AUTHOR]

The fall armyworm (FAW), Spodoptera frugiperda (J.E. Smith) (Lepidoptera: Noctuidae), is an important insect pest of maize (Zea mays L., Poaceae). The use of synthetic insecticides to control this pest remains the most popular option amongst growers. Natural insecticides offer a safer alternative to synthetic insecticides for controlling FAW. This study aimed to investigate the lethal and sublethal effects of a calcium carbonate formulation against this invasive insect pest. The tested formulation was a mixture of calcium carbonate (five concentrations), wetting agent and dispersant agent. Four experiments were conducted including a direct contact test, a no‐choice feeding test, a choice feeding test and an oviposition test. The application of the calcium carbonate formulation on maize leaves had a strong anti‐oviposition effect for adult female FAW, but a low‐to‐moderate antifeedant effect on FAW larvae. The growth and development of FAW larvae were only slightly affected by the calcium carbonate formulation in the feeding and the direct contact tests. The lethal effects of the calcium carbonate formulation against FAW larvae were low in direct contact and feeding tests. These results indicate that a calcium carbonate formulation has the potential to be used as the push component in a push–pull strategy for FAW control. Considering its low toxicity, both through direct contact and feeding, calcium carbonate can be an environmentally friendly alternative for the management of FAW. [ABSTRACT FROM AUTHOR]

Dryocosmus kuriphilus, commonly known as the chestnut gall wasp, belongs to the family Cynipidae and is native to China. It is a highly invasive insect species causing serious damage to chestnut trees and has rapidly spread to various continents, including Europe, North America, and Oceania. The D. kuriphilus has become one of the important pests of chestnut plants in the world and is listed as a quarantine object by the European and Mediterranean Plant Protection Organization (EPPO). In this study, we used PacBio long reads, Illumina short reads, and Hi-C sequencing data to construct a chromosome-level assembly of the D. kuriphilus genome. The assembled genome includes 14,729 contigs with a total length of 2.28 Gb and a contig N50 of 0.8 Mb. With Hi-C technology, 2.17 Gb (95.02%) of contigs were anchored and oriented into the 10 pseudochromosomes with the scaffold N50 of 198.8 Mb and the scaffold N90 of 158.8 Mb. In total, 24,086 protein-coding genes were predicted in the assembled D. kuriphilus genome as the reference gene set. A total of 1.82 Gb repeats (occupying 79.7% of the genome), including 1.42 Gb of transposable elements and 0.40 Gb of tandem repeats, were identified in D. kuriphilus genome. In the evaluation of completeness, the BUSCO analysis determined a level of 98.1% completeness for the assembled genome sequences based on the Insecta database (OrthoDB version 10). The high-quality genome assembly of D. kuriphilus will not only provide a valuable reference for the study of its evolutionary history and genetic structure but also facilitate the research of host-pest interactions and invasiveness. Moreover, this genome assembly will promote in the development of effective management strategies to mitigate the economic and ecological impacts of this invasive pest on chestnut trees and ecosystems. [ABSTRACT FROM AUTHOR]

Simple Summary: This study explores the diversity of ladybird beetles on San Cristóbal Island, Galápagos Archipelago. We found nineteen species, including four already known and nine new ones reported for the first time on the island. Our research showed that endemic species, unique to the Galápagos, are rare and primarily found in native forests, highlighting their need for protection. The native species Cycloneda sanguinea was the most common and adaptable. Non-native species, like Cheilomenes sexmaculata, were found across all disturbed areas, including urban and agricultural zonas, and may be spreading into natural habitats. This information is crucial for conserving the Galápagos' delicate ecosystems by emphasising the importance of monitoring and managing native and non-native insect species to preserve local biodiversity and prevent adverse environmental impacts. This study investigates the species richness and distribution of ladybird beetles (Coccinellidae) across various habitats on San Cristóbal Island in the Galápagos Archipelago, Ecuador. Through extensive field surveys, we catalogued nineteen species, including four previously known species (two endemics, Psyllobora bisigma and Scymnobius scalesius, and two natives, Cycloneda sanguinea and Tenuisvalvae bromelicola). We also identified nine possibly native species reported for the first time in the Galapagos islands in this study or correspond to the first voucher specimens for the island. We collected three previously reported non-native species: Cheilomenes sexmaculata, Novius cardinalis, and Paraneda guticollis. Three species belonging to the genera Stethorus, Calloeneis, and Delphastus remain undetermined, pending further taxonomic analyses. Our findings reveal a rich and complex community with notable differences in species abundance and habitat preference. Endemic species were found to be particularly scarce and restricted mainly to crops undergoing forest regeneration and deciduous forests, emphasising their vulnerability and specialised habitat requirements. The native Cycloneda sanguinea emerged as the most prevalent species, exhibiting broad ecological adaptability. Non-native species, like Cheilomenes sexmaculata, were predominantly found in disturbed habitats, with some showing early signs of spreading into more natural environments, raising concerns about their potential impact on local biodiversity. These findings contribute valuable knowledge to understanding Coccinellidae diversity on San Cristóbal Island and highlight the importance of continued monitoring, particularly in the face of ongoing environmental change and the introduction of non-native species. This study underscores the need for targeted conservation efforts to protect the unique and fragile ecosystems of the Galápagos Archipelago. [ABSTRACT FROM AUTHOR]

Simple Summary: Spodoptera frugiperda is an important economic pest that has recently invaded Africa and Asia. This study systematically assessed its life history traits under varying temperatures, photoperiods, and humidity levels. The results show that its physiological activity is highest at 32 °C, with reduced durations of the developmental stages, increased oviposition quantity and period in females, and a shorter pupal stage, which extends the adult lifespan. These findings help predict population dynamics and inform management strategies. Spodoptera frugiperda (J.E. Smith) is a significant economic pest that has recently invaded Africa and Asia. However, much of the information regarding its ecological capabilities in these newly invaded environments remains largely unknown. In this study, the life history traits of the fall armyworm under conditions of increased temperature, different photoperiods, and varying humidity levels were systematically evaluated. Among 43 studies, a total of 20 studies were included in the analysis by passing the screening criteria, and random-effects meta-analysis, fixed-effects meta-analysis, and meta-regression were conducted. It has been found that with the increase in temperature above 20 °C, various physiological indicators of the fall armyworm are significantly enhanced. When the temperature reaches 32 °C, the physiological activities of S. frugiperda are at their highest point. As the temperature increases, the duration of each developmental stage of the fall armyworm decreases significantly, accompanied by an increase in oviposition quantity and period in females. Additionally, the pupal development time is shortened, which leads to an increase in the lifespan of the adult moth. Using temperature and relative humidity as environmental variables, the optimal survival conditions for each insect state of the fall armyworm were calculated. These findings can assist in predicting the population dynamics of the fall armyworm and in formulating appropriate and practical management strategies. [ABSTRACT FROM AUTHOR]

The solanum fruit fly Bactrocera latifrons (Diptera: Tephritidae) is an invasive alien insect that causes huge economic losses to pepper and other solanaceous plant industries. It is mainly distributed in South and Southeast Asia, SW Europe, Western USA, and in some African countries. However, the potential global geographical distribution of B. latifrons is unknown. Therefore, in this study, based on the current (1981–2010) and future (2040–2059) climatic scenarios determined using the CNRM-CM5, Access1.0, GFDL-ESM-2M, and NorESM1-M models, we used a species distribution model (CLIMEX 4.0) to project the potential global geographic distribution of B. latifrons to prevent further invasion and harm. In the current climate scenario, South America (1286.06 × 104 km2), Africa (1435.47 × 104 km2), and Oceania (410.66 × 104 km2) have the largest proportions of suitable land areas for B. latifrons colonization. Under all four future climate models, the global potential suitable area for Bactrocera latifrons is projected to decrease and shift towards higher latitudes. This study provides an important baseline upon which researchers, quarantine personnel, and governments can develop the appropriate control strategies against B. latifrons. [ABSTRACT FROM AUTHOR]

ABSTRACT Insects have evolved a spectrum of strategies that facilitate survival in the face of adverse environmental conditions and bottom‐up or top‐down pressures. The egg is the first stage in the life cycle of most insects. It is not only immobile but in many insects is the stage that survives unfavourable seasons when food resources are unavailable. Eggs are targeted by oophagous natural enemies and also are subject to abiotic stresses. In response to these diverse stresses, insects have developed various egg protection strategies. Females of many insects lay eggs in clusters and then use their own body resources to cover them to provide protection from harsh environments and biotic attack. Such egg protection strategies have allowed some herbivorous insects to thrive in new environments and become serious invasive pests. Females of many insects protect their eggs in other ways (e.g. laying eggs in concealed places, direct parental care) while others do not provide protection at all. Here, we review various egg protective strategies in insects. Our focus is on adaptive ecological mechanisms and temporal variation as well as the benefits and costs of egg coverings. We highlight several case studies on how these egg protective traits might impede biological control of globally important agricultural and forest pests and propose a framework for incorporating egg protective traits into biological control programs especially for invasive insect pests. [ABSTRACT FROM AUTHOR]

Tuta absoluta, known as the South American tomato leaf miner, significantly impacts tomato plants (Solanum lycopersicum) economically on a global scale. This pest, belonging to the Gelechiidae family, is native to South America and was first identified in Peru in 1917. Since its discovery, T. absoluta has rapidly spread to Europe, Africa, and Asia, severely threatening tomato production in these regions. The widespread application of chemical pesticides against this pest has resulted in significant environmental harm, including contamination of soil and water, and has had negative effects on non-target species such as beneficial insects, birds, and aquatic life. Although substantial research has been conducted, biological control methods for T. absoluta remain insufficient, necessitating further study. This review covers the Biology, Classification, and Entomopathogen-Based Management of T. absoluta (Meyrick) in Asia. It provides essential insights into the pest's life cycle, ecological impacts, and the potential of entomopathogens as biocontrol agents. The detailed information presented aims to facilitate the development of sustainable pest control strategies, minimizing environmental impact and promoting the use of entomopathogens as viable alternatives to chemical pesticides in controlling T. absoluta insect pest. [ABSTRACT FROM AUTHOR]

Earth is now experiencing declines in insect abundance and diversity unparalleled in human history. The drivers underlying those declines are many, complex, and incompletely known. Here, using a natural experiment, we report the first test of the hypothesis that forest defoliation by an invasive outbreak insect compromises the fitness of a native insect via damage‐induced increases in toxicity of the forest canopy. We demonstrate that defoliation by the invasive spongy moth (Lymantria dispar) elicits an average 8.4‐fold increase in foliar defense expression among aspen (Populus tremuloides) genotypes. In turn, elevated defense dramatically reduces survivorship, feeding, and growth of a charismatic mega moth (Anthereae polyphemus). This work suggests that changes to the phytochemical landscape of forests, mediated by invasive outbreak insects, are likely to negatively impact native insects, with potential repercussions for community diversity and ecosystem function across expansive scales. [ABSTRACT FROM AUTHOR]

In 2023, two bark beetle species were recorded in Russia for the first time based on findings in Sochi: the cypress bark beetle, Phloeosinus armatus, on Mediterranean cypress (Cupressus sempervirens) and the black twig borer, Xylosandrus compactus, on southern magnolia (Magnolia grandiflora), noble laurel (Laurus nobilis), and cherry laurel (Prunus laurocerasus). P. armatus is a Mediterranean pest attacking exclusively Cupressaceae. In Sochi, it was recorded on cypress trees in several locations, including on plants for planting imported from Italy. X. compactus is a highly polyphagous pest from East Asia. In Sochi, it has established populations in ornamental planted areas. The paper provides species descriptions with identification keys. One specimen of X. compactus from Sochi was DNA‐barcoded and compared with 31 specimens deposited in the BOLD and GenBank under the name of X. compactus. High genetic variability (>7%) was detected highlighting the difficulty of identifying 'true' X. compactus using DNA data. Genetically, the specimen of X. compactus from Sochi was identical to those from Italy, Spain and France. An accidental introduction with ornamental plants for planting from Italy is considered the most probable pathway of invasion of both bark beetle species into the Russian Black Sea coast of the Caucasus. [ABSTRACT FROM AUTHOR]

Increasing temperatures, prolonged drought, the increased severity and intensity of storms, and other effects of climate change are being felt globally, and long-lived forest tree species may struggle in their current ranges. Oaks (Quercus spp.) have evolved a range of adaptations to dry and hot conditions and are believed to be a "climate change winner" by increasing their suitable habitat. However, a mixture of life history traits and increasing susceptibility to herbivores and xylovores as well as secondary pathogen infections still put oaks at risk of decline. Oak species found in the Midwestern, Great Lakes, and Northeastern United States and Canada are important keystone species with high ecological and economical importance. They are also vulnerable to existing, new, and emerging threats that have the potential to cause mortality across entire stands quickly. Current examples of insect threats include the Lymantria dispar (spongy moth), Agrilus bilineatus (twolined chestnut borer), and Nitidulidae (sap beetles) as disease vectors. Examples of emerging insects of concern include Cynipidae (oak gall wasps) and Enaphalodes rufulus (red oak borer). This study describes these insects, explains their mechanisms of action and the effects on oaks, and explores mitigation strategies for each. [ABSTRACT FROM AUTHOR]

The evolution of oviposition preference in insects is considered a key evolutionary strategy in the context of host–plant interaction. It is hypothesized that insects maximize the survival and fitness of the subsequent generations by preferring specific host plant(s), known as the "preference-performance hypothesis." In this study, we tested whether adult host preference reflects the immature performance in an oligophagous insect, Phthorimaea absoluta Meyrick, a rapidly emerging invasive pest in Asia, Africa, and Europe. Based on a preliminary survey of the potential host plants of P. absoluta , we selected 6 Solanaceae species, namely, tomato, potato, eggplant, black nightshade, sweet pepper, and tobacco, for the oviposition preference studies. The results indicated that the tomato was the most preferred host in no-, dual- and multiple-choice assays, followed by potato, eggplant, and black nightshade. Subsequently, the insect life-table parameters were found to be superior on tomato compared to other hosts. The order of oviposition preference on the host plants was strongly correlated with the life-table parameters of P. absoluta. Thus, we provide clear evidence for the preference–performance hypothesis in the host selection behavior of P. absoluta. We also emphasize the necessity of conducting oviposition behavior research at various geographic locations to develop tailor-made integrated pest management programs. [ABSTRACT FROM AUTHOR]

Hylurgus ligniperda invaded Shandong, China, through imported forest timber, posing a threat to China's forest health. Exotic insects with broad environmental tolerance, including low temperatures, may have a better chance of surviving the winters and becoming invasive. Understanding the cold-tolerance strategies of H. ligniperda may help to design sustainable pest management approaches. In this study, we aim to investigate the cold-tolerance ability and relevant physiological indicators in overwintering H. ligniperda adults to determine any possible overwintering strategies. Supercooling points (SCPs) for adults H. ligniperda differed significantly across months and reached the lowest level in the mid- and post-overwintering period, the minimum SCPs −6.45 ± 0.18 °C. As the cold exposure temperature decreased, the survival rate of adults gradually decreased, and no adult survived more than 1 day at −15 °C, and the LLT50 for 1 day was −7.1 °C. Since H. ligniperda adults can survive internal ice formation, they are freeze-tolerant insects. Throughout the overwintering period, the SCPs and the water, protein, sorbitol, and glycerol content in adults decreased initially and then increased. We reported significant correlations between total protein, sorbitol, trehalose, and glycerol content in the beetles and SCPs. Glycogen, lipid, protein, trehalose, and sorbitol content in adult beetles may directly affect their cold-tolerance capacity and survival during winter. This study provides a physiological and biochemical basis for further study of metabolism and cold-tolerance strategies in H. ligniperda adults, which may help predict population dynamics and distribution potential of pests. [ABSTRACT FROM AUTHOR]

This document is a list of references for a research article titled "Adaptation strategies of insects to their environment by collecting and utilizing external microorganisms." The article explores the ways in which insects interact with microorganisms in their environment and how these interactions contribute to their adaptation and survival. The references cover a range of topics related to insect-microorganism interactions, including the role of microorganisms in insect herbivory, the antimicrobial potential of insect-associated bacteria, the impact of microorganisms on insect behavior, and the use of microorganisms for biological control of pests. The article emphasizes the importance of understanding these interactions for sustainable pest management and highlights the potential for using insect-associated microorganisms in various applications, such as antibiotic production and biomass degradation. [Extracted from the article]

Introduction: In the invaded areas, it is believed that invasive species reduce their investment in defense due to the absence of natural enemies. Methods: By field investigation and a series of laboratory assays, This study explored the defense strategies of invasive plants. Results: Field investigation indicated that invasive plants have a antifeedant effect on herbivorous pests, and the distribution frequency of wormholes of native plants shows a peak at a distance of 2-3m from the invasive species. The feeding preference experiment conducted with two generalist herbivorous insects (native insect Spodoptera litura and invasive insect Spodoptera frugiperda) showed that the invasive plants have a stronger antifeedant effect than native plants. By analyzing the content of secondary metabolites in the leaves of three invasive plants (Sphagneticola trilobata, Mikania micrantha, Ipomoea cairica) and three native plants (Ipomoea nil, Paederia foetida, Polygonum chinense), the leaves of invasive plants had higher concentrations of substances associated with defenses, including total phenols, flavonoids, jasmonic acid, tannin, H2O2, and total antioxidant capacity (TAC), and lower soluble protein concentrations than native plants. After leaf damage, compared to native plants, the leaves of invasive plants showed an overall increase in substances associated with defense, except for soluble sugar. Discussion: These results suggest that invasive plants maintain active defense strategies in invaded areas, leading to changes in the distribution patterns of herbivorous insects in a manner that facilitates invasion. [ABSTRACT FROM AUTHOR]

The recent biological invasion of box tree moth Cydalima perspectalis on Buxus trees has a major impact on European boxwood stands through severe defoliation. This can hinder further regrowth and threaten survival of populations. In a mesocosm approach and controlled larval density over a 2‐month period, responses of B. sempervirens essential and specialized metabolites were characterized using metabolomics, combining 1H–NMR and LC–MS/MS approaches. This is the first metabolome depiction of major Buxus responses to boxwood moth invasion. Under severe predation, remaining green leaves accumulate free amino acids (with the noticeable exception of proline). The leaf trans‐4‐hydroxystachydrine and stachydrine reached 10–13% and 2–3% (DW), while root content was lower but also modulated by predation level. Larval predation promoted triterpenoid and (steroidal) alkaloid synthesis and diversification, while flavonoids did not seem to have a relevant role in Buxus resistance. Our results reveal the concomitant responses of central and specialized metabolism, in relation to severity of predation. They also confirm the potential of metabolic profiling using 1H–NMR and LC–MS to detect re‐orchestration of metabolism of native boxwood after severe herbivorous predation by the invasive box‐tree moth, and thus their relevance for plant–insect relationships and ecometabolomics. [ABSTRACT FROM AUTHOR]

Simple Summary: The colonization of new areas by invasive insects has been a prominent concern in entomology. Bactrocera dorsalis serves as a typical invasive insect, which has expanded its range from southern to northern regions of China in recent years. Host suitability plays a crucial role in the successful establishment of B. dorsalis populations. In this study, we have observed a decline in the fitness of B. dorsalis when feeding on cucumber, primarily due to significant changes in the gut microbiota composition of the insect. Among them, Empedobacter brevis and Enterococcus faecalis were identified as key factors leading to the reduced fitness of B. dorsalis. These findings confirm the close association between insect fitness and symbiotic bacteria within the insect's body. Furthermore, the source of food and gut bacteria have significant impacts on the invasion of exotic insects. How alien pests invade new areas has always been a hot topic in invasion biology. The spread of the Bactrocera dorsalis from southern to northern China involved changes in food sources. In this paper, in controlled conditions, we take Bactrocera dorsalis as an example to study how plant host transformation affects gut bacteria by feeding it its favorite host oranges in the south, its favorite host peaches and apples in the north, and feeding it cucumbers as a non-favorite host plant, thereby further affecting their fitness during invasion. The result showed that, after three generations of feeding on cucumbers, Bactrocera dorsalis took longer to develop as a larva while its longevity and fecundity decreased and pre-adult mortality increased. Feeding it cucumbers significantly reduced the overall diversity of gut microbiota of Bactrocera dorsalis. The relative abundance of Enterobacter necessary for survival decreased, while the Empedobacter and Enterococcus increased, resulting in decreased carbohydrate transport and metabolism and increased lipid transport and metabolism. Feeding Bactrocera dorsalis Empedobacter brevis and Enterococcus faecalis resulted in a 26% increase in pre-adult mortality and a 2–3 d increase in adult preoviposition period (APOP). Additionally, Enterococcus faecalis decreased the longevity of female and male adults by 17 and 12 d, respectively, and decreased fecundity by 11%. We inferred that the shifted plant hosts played an important role in posing serious harm to Bactrocera dorsalis invading from the south to the north. Therefore, after an invasion of Bactrocera dorsalis into northern China, it is difficult to colonize cucumbers for a long time, but there is still a risk of short-term harm. The findings of this study have established that the interactions between an insect's food source and gut bacteria may have an important effect on insect invasions. [ABSTRACT FROM AUTHOR]

Aim: Invasions of non‐native insects can have substantial impacts on agriculture, forestry, human health and biodiversity with considerable economic and environmental consequences. To understand the causes of these invasions, it is important to quantify the relative influence of principal drivers such as international imports and climatic effects. Location: North America, Chile, Europe, Australia, New Zealand and Japan. Time Period: 1881–2020. Methods: To evaluate the relative contributions of various factors in explaining global variation in numbers of non‐native insect establishments in different world regions, we conducted two multivariate regression analyses to quantify temporal changes in family‐level composition and native ranges of established non‐native species in several world regions. Results: There were temporal changes in the family‐level composition of non‐native species assemblages. Prior to 1900, invasions were dominated by scale insects, subsequently shifting to a more diverse set of species, except in North America, which had relatively small compositional change over time compared to other regions. Spatial and temporal variation in the composition of established species was associated with differences in the origin of imports and climatic factors, each explaining 26.3% and 27.4% of the total variation, respectively. The analysis of native ranges of non‐native species indicated that there was no consistent temporal variation across all regions. Established species in New Zealand were predominantly native to Australasia and species in North America and Chile were mainly from Europe. Non‐native species in Europe mainly originated from the Nearctic region while those in Japan and Australia generally originated from multiple regions. Climatic factors in the destination regions had a primary effect (66.3%) on variation in the native range of established species, although imports also had substantial effects (45.4%). Main Conclusions: Geographical variation in climate and imports act together as drivers of establishment success for non‐native insects in all six regions. [ABSTRACT FROM AUTHOR]

Spodoptera frugiperda, an invasive pest insect that targets maize and other crops, first arrived in Japan in the summer of 2019. This species occurs year‐round in East Asian subtropical regions such as southern mainland China and the island of Taiwan, where the mean air temperature in the coldest month is above 10°C. Adults are similarly found throughout the year on the southwestern islands of Japan. Trap monitoring there showed continuous or intermittent S. frugiperda catches in the three winter seasons since 2019. However, it was difficult to distinguish between immigrants arriving from these neighbouring areas and local individuals occurring on each Japanese island. In this study, the possible natal origin of captured insects on five small islands (Yonagunijima, Taramajima, Okinawajima, Amamioshima and Tanegashima) was determined by investigating the strontium radiogenic isotope ratios (87Sr/86Sr) and comparing them with those of reference hosts and insects. Since trapping data and the 87Sr/86Sr values of trapped insects didn't support S. frugiperda's winter breeding on the northernmost island, Tanegashima, further analysis was limited to the four southern islands. The 87Sr/86Sr values of reference host plants and reared insects on the four islands ranged from 0.70929 to 0.71009, while those of catch insects ranged from 0.70885 to 0.71090. The 87Sr/86Sr values of the catch insects and the reference on the four islands did not differ significantly. In addition, the monthly averages of daily mean air temperature in January and February 2020–2022 were above 10°C, and the wind direction at the surface was mostly from the northeast or northwest. These pieces of evidence, together with winter host availability, suggested that S. frugiperda occurs year‐round on the islands. In other words, the year‐round occurrence area of S. frugiperda in East Asia extends to the Japanese southwestern islands below Amamioshima Island. [ABSTRACT FROM AUTHOR]

The introduction of wood-boring insects to non-native areas can pose a significant threat to global forest ecosystems. Aromia bungii and Massicus raddei, native to Asia including Korea but potentially important in urban forestry worldwide, are severe trunk borers that have recently infested host trees in urban landscapes of Korea. The signs of external injury can help identify cerambycid-infested trees. However, assessing the internal condition of trees without causing damage is challenging, which hinders the management of infested trees that are still alive. This study evaluated the potential use of sonic tomography to assess injury characteristics in the woody parts of trees, such as Prunus × yedoensis and Quercus palustris infested by A. bungii and M. raddei, respectively. Among the 45 investigated trees, the number of holes on external parts was recorded by visual examination. Sonic tomography images revealed decayed areas, allowing for a comparison of internal damage between infested and non-infested trees. The internal status of trees infested by the two cerambycid beetles differed from that of healthy trees. While some healthy trees exhibited minimal internal damage, those that were not infested by beetles showed decayed areas, potentially caused by non-biological factors such as frozen cracks. In conclusion, sonic tomography equipment can potentially be used for non-invasive assessment of internal injuries caused by wood-boring insects in urban trees. [ABSTRACT FROM AUTHOR]

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BACKGROUND: Temperature is a primary factor that determines the eco‐geographical distribution and population development of invasive insects. Temperature stress leads to various negative effects, including excess reactive oxygen species (ROS), and catalase (CAT) is a key enzyme against ROS in the antioxidant pathway. The whitefly Bemisia tabaci MED is a typical invasive pest that causes damage worldwide. Our previous studies have shown that CAT promotes whitefly adaptation to high temperature by eliminating ROS. However, the mechanism underlying the low‐temperature adaptation of whiteflies is still unknown. RESULTS: In this study, we investigated the role of CAT in the low‐temperature tolerance of B. tabaci MED by analyzing its survival rate, reproduction, and ROS levels at 25 °C (as a control, suitable temperature), 20 °C (moderately decreased temperature), and 4 °C (severely decreased temperature). Silencing of BtCAT1, BtCAT2, or BtCAT3 reduced the viability of whiteflies under a short‐term severely decreased temperature (4 °C), which manifested as decreases in survival and fecundity accompanied by significant increases in ROS levels. Moreover, even at a moderately decreased temperature (20 °C), silencing of BtCAT1 led to high ROS levels and low survival rates in adults. CONCLUSION: Silencing of BtCATs significantly increased the sensitivity of B. tabaci MED to low temperatures. BtCAT1 is likely more essential than other BtCATs for low‐temperature tolerance in whiteflies. © 2024 Society of Chemical Industry. [ABSTRACT FROM AUTHOR]

The introduction in the same area of different invasive species can result in novel interactions, with unpredictable consequences. We carried out a study in Galicia (northwestern Iberian Peninsula) with the aim of clarifying the interactions between two invasive species Cryphonectria parasitica and Dryocosmus kuriphilus. In 2018, we selected five chestnut plots affected by both species. We compared trees affected only by the insect and trees affected by both the insect and the fungus with respect to attack level, gall characteristics, female size and fecundity, and concentrations of nitrogen, water and secondary metabolites. We also evaluated female preferences in a greenhouse assay. There were higher levels of attack in trees affected by both invaders. However, the greenhouse assay showed that ovipositing females do not preferentially choose trees attacked by the fungus. The presence of the fungus had no effect on the size, wall thickness, or hardness of D. kuriphilus galls, but larvae were smaller in trees also affected by the fungus. The fecundity of females was strongly related to the presence of chestnut blight; the number of eggs per female was almost double in trees affected by the fungus. There were no relations between blight and the nitrogen or water content in the galls, where the insects feed. There were also no effects of chestnut blight on the concentration of terpenes or phenols, but condensed tannins were higher in trees with chestnut blight. The higher tannins induced by chestnut blight may directly or indirectly benefit gall wasps. Positive relationships between condensed tannin concentration and reproductive performance of other gall makers were previously reported. Tannins can also improve the negative effects of environmental conditions inside the gall. Our results indicate that the presence of chestnut blight can increase the suitability of chestnut trees for the invasive insect, D. kuriphilus, through the increase in tannins due to the presence of the fungus. [ABSTRACT FROM AUTHOR]

Invasive wood-boring insects are a major economic and ecological concern worldwide as they impact native woody plant populations. These pest species are increasing in prevalence, with devastating impact, as global trade leads to higher rates of introduction and establishment. The emerald ash borer (Agrilus planipennis; EAB) is one such species, which has caused widespread damage across much of the United States and is now spreading across Europe. Non-indigenous woodborers such as EAB are difficult to detect at early stages of invasion, which is when management and eradication efforts are most effective and cost efficient. Environmental DNA (eDNA) surveys have demonstrated power in detecting invasive species when rare in the landscape due to their ability to detect trace amounts of DNA and identify to species. Here, we trialled a novel eDNA method for collecting environmental samples within host trees where invasive pest larvae are feeding, using EAB as a case study. We extracted tree cores approximately 1 cm in length using an increment hammer to assess detectability of eDNA from larvae feeding under the bark. In trees visibly infested with EAB, we observed a seasonal peak in EAB DNA detection probability (~ 64%; towards the end of the growing season), indicating a potential impact of ash tree phenology or EAB phenology on detection. When we trialled the method in a site with ash trees of low or uncertain EAB abundance, we did not record positive EAB eDNA detections. This outcome may have resulted from differing EAB phenology at the northern latitude of this survey site or because larval galleries were less numerous causing EAB DNA to be scarcer within the tree. Our results, however, provide preliminary evidence that increment hammer tree cores can be used to detect eDNA of EAB and, perhaps, other wood-boring pests. Further work is needed to clarify false negative survey detections at ash trees showing little to no signs or symptoms of infestation, as well as investigating the deposition, transport and persistence dynamics of EAB eDNA within trees. [ABSTRACT FROM AUTHOR]

Fall armyworm (FAW), Spodoptera frugiperda (J. E. Smith) (Lepidoptera: Noctuidae), is a serious invasive insect pest affecting global maize production. Effective integrated management of FAW is essential to minimize the yield losses. The objective of this study is to determine a suitable package for the sustainable management of FAW by validating the potentiality of various integrated approaches through field trials. In the present study, five different integrated pest management treatments consisted of different components were synthesized and evaluated against fall armyworm in maize-based systems during winter (December 2022–April 2023) and rainy (July–November 2023) seasons. The data on the number of plants damaged, leaf damage rating (1–9 scale), and natural enemies such as spiders, coccinellids, and earwigs were recorded from 20 randomly selected plants at 7 and 14 days after the first and second sprays. Yield data (q ha−1) were recorded at the time of harvest. Treatment 1 consisting of pheromone traps at a rate of 4 per acre with ICAR-NBAIR lures, erection of bird perches at a rate of 10 per acre, seed treatment with Chlorantraniliprole 50 FS at a rate of 5.6 ml per kilogram of seed, and spray application of azadirachtin 1500 ppm at the rate of 5 ml per litre, and Metarrhizium anisopliae with spore count of 1 × 108 cfu/g (1 kg per acre) at a rate of 5 g per litre significantly reduced the per cent plant infestation (12.7) and leaf damage rating (2.1) by FAW larvae compared to untreated control (39.7, 4.1), respectively. The higher natural enemy population (spiders, coccinellids, and earwigs) was also observed in Treatment 1 (8.8) compared to Treatment 5 (4.1) (chemical control). Furthermore, higher grain yield of 51.5 q ha−1 was obtained in Treatment 1 with a cost–benefit ratio of 1:2.1, whereas in untreated control, the yield obtained was 29.0 q ha−1 with a cost–benefit ratio of 1:1.3. The highest per cent of avoidable yield losses of 43.6 was observed in Treatment 1 in comparison with other treatments. Integration of sustainable management approaches reduces the application of chemical insecticides and enhances the population of natural enemies which would be beneficial to maize farmers. [ABSTRACT FROM AUTHOR]

Crapemyrtle bark scale (CMBS), Acanthococcus lagerstroemiae (Kuwana) (Hemiptera: Eriococcidae), is a non-native scale insect that has spread throughout many urban areas of the Southeast and Middle Atlantic regions of the United States following its initial detection near Dallas, Texas in 2004, severely reducing the aesthetic value and health of the popular ornamental crapemyrtle tree (Lagerstroemia spp.). We infested crapemyrtles with known numbers of CMBS to determine the minimum number of individuals required for establishment after initial arrival on plants. We also investigated how netting—implemented to understand differences in establishment when scale dispersal and predation are inhibited—influenced population growth. We determined that one female CMBS egg sac can successfully establish a new population ~ 92% of the time and that netting had negligible effects on establishment. Our results underscore the importance of surveying and managing CMBS and scale insects with similar biology when attempting to prevent infestation of nursery stock, which is widely implicated as a vector for long-distance dispersal of scale insects. [ABSTRACT FROM AUTHOR]

The phylogeography of invasive pests is fundamentally important for the reconstruction of invasion pathways. Infection patterns with maternally inherited endosymbionts such as Cardinium and Wolbachia may provide additional resolution power. Kelly's citrus thrips, Pezothrips kellyanus, a significant Australian-native pest of citrus, has independently colonised New Zealand and the Mediterranean region. However, the Australian source populations for these two invasive ranges are unknown. Furthermore, Australian populations have previously been found with Cardinium and Wolbachia coinfections. Surprisingly, invasive-range populations only had Cardinium but not Wolbachia, although both endosymbionts independently cause cytoplasmic incompatibility that facilitates the endosymbionts' spread in host populations. To resolve this puzzle, we examined mitochondrial diversity and endosymbiont prevalence in 59 P. kellyanus populations across the native and invasive ranges. We found that populations from Victoria in Australia were Wolbachia-free and shared mitochondrial haplotypes with invasive-range populations, suggesting they were the likely source of the founder populations in both invasive ranges. However, we also detected a linkage pattern between Wolbachia and particular haplotypes in Australia. Furthermore, populations with a high Wolbachia prevalence in eastern Australia had a substantially lower haplotype diversity than populations with low Wolbachia prevalence in other parts of Australia. This indicates a Wolbachia-driven selective sweep of mitochondria in the native range of this haplodiploid host due to maternal coinheritance. In contrast, Cardinium was associated with all haplotypes, demonstrating an older association with Cardinium than Wolbachia. Overall, while endosymbionts are informative for tracing pest invasions, the selective sweeps they cause can potentially confound phylogeographic patterns and invasion pathways. [ABSTRACT FROM AUTHOR]

Simple Summary: Tuta absoluta (Meyrick) is an invasive insect pest that poses a threat to solanaceous crops. In recent years, the occurrences of extremely high temperature have been increasing due to climate change. Therefore, it is important to study the effect of high temperature on the growth and development of T. absoluta. In this study, when the eggs, pupae, and adults of T. absoluta were exposed to high-temperature stress, the pupal stage exhibited the highest sensitivity. This was demonstrated by a significant decrease in emergence rate, longevity, and fecundity. High-temperature stress during the egg stage resulted in a cascading effect, leading to a reduction in the adult longevity. This study provides a new insight for the integrated management of T. absoluta under high temperature. Insect life processes and reproductive behaviors are significantly affected by extremely high temperatures. This study focused on Tuta absoluta, which poses a severe threat to tomato cultivars. The effects of intense heat stress on the growth, development, oviposition, and longevity of T. absoluta were investigated. This investigation encompassed various developmental stages, including eggs, pupae, and adults. This study revealed that egg hatching and pupa emergence rates were significantly reduced at a temperature of 44 °C maintained for 6 h. The longevity of adults that emerged after the egg and pupal stages were exposed to 44 °C for 6 h was significantly reduced compared to the control. Notably, there was no significant variation in adult fecundity after egg-stage exposure to high temperatures. However, all treatments exhibited significantly reduced fecundity compared to the control after exposure to high temperatures during the pupal stage. Adult survival rates after exposure to 40 °C and 44 °C for 3 h were 74.29% and 22.40%, respectively, dramatically less than that of the control, which was 100%. However, no significant differences were noted in terms of longevity and egg production. These results offer a better understanding of the complex interactions between extreme temperatures and the life history traits of T. absoluta, thereby offering valuable insights for implementing management strategies to alleviate its impact on tomato crops in response to climate change. [ABSTRACT FROM AUTHOR]

Simple Summary: In South Korea, the increase in growing tropical and subtropical crops due to climate change and consumer demand has brought along a challenge: invasive insect pests damaging these fruits. To address this issue, we explored using ethyl formate (EF) fumigation as a safer alternative to traditional methods. We focused on two types of aphids, common pests in the trade of fresh produce. We found that EF effectively controlled aphids across different stages of fruit cultivation and post-harvest storage, achieving 100% mortality without harming the plants. Importantly, EF levels rapidly decreased after fumigation, ensuring safety. These findings suggest that EF fumigation could be a valuable solution for protecting crops from pests in a changing climate. This research provides farmers and policymakers with a safer and more sustainable method for pest control in greenhouse and post-harvest storage, ensuring the availability and quality of tropical fruits for consumers while reducing environmental risks associated with traditional pesticides. Tropical and subtropical crops are being increasingly cultivated in South Korea, leading to an increase in damage by exotic insect pests. Consequently, ethyl formate (EF) is currently being considered for quarantine and pre-shipment fumigation. In this study, we evaluated the effectiveness of EF fumigation for controlling Aphis spiraecola Patch and Aphis gossypii Glover, two representative quarantine pests on passion fruit ("Pink Bourbon") during greenhouse cultivation and post-harvest storage. The efficacy of EF against both aphids in terms of the lethal concentration causing 50% mortality (LCt50%) and LCt99% was 1.36–2.61 g h/m3 and 3.73–7.55 g h/m3 under greenhouse conditions (23 °C), and 1.37–2.02 g h/m3 and 3.80–14.59 g h/m3 post-harvest (5 °C), respectively. EF at 4 g/m3 for 4 h resulted in 100% mortality of A. spiraecola, which was more resistant to EF, without causing phytotoxic damage to the trees in a 340 m3 greenhouse. Post-harvest fruit fumigation at 10 g/m3 for 4 h in a mid-size (0.8 m3) fumigation chamber resulted in complete disinfection. Moreover, the EF level decreased below the EF threshold within 10 min after natural ventilation in the greenhouse. Therefore, our results suggest EF fumigation as an effective method for controlling A. spiraecola and A. gossypii. [ABSTRACT FROM AUTHOR]

Along with climate change, or by transportation of agricultural products, such as fruits and ornamental plants, their alien insect pests have also started to appear in Hungary. Although these pest species are sometimes sparsely studied, their biology and interactions with their host plants provide key information for monitoring their spread and identifying their vulnerable points, targets for developing methods preventing their overpopulation. In this review article we have collected data on newly emerged moth species recently found in Hungary that might threaten plantations in Hungary in the near future. [ABSTRACT FROM AUTHOR]

Nipa palm hispid beetle (Octodonta nipae) is an insect species that is native to Malaysia but has spread to southern China and beyond, seriously threatening palm production. A lack of high-quality genome resources has hindered understanding of the insect's invasive characteristics and ecological adaptations. Here, we combined Illumina short read, PacBio long-read, and high-throughput chromosome conformation capture (Hi-C) sequencing technologies to generate a high-quality, chromosome-scale genome assembly of nipa palm hispid beetle. The genome assembly was 1.31 Gb in size, consisting of nine chromosomes. The contig and scaffold N50 values were 1.022 Mb and 148.6 Mb, respectively. The genome assembly completeness was estimated at 99.1%. Annotation revealed 16,305 protein-coding genes and 62.16% repeat sequences. This high-quality genome assembly is a valuable resource that will contribute to understanding of the genetic factors underlying the invasive characteristics of nipa palm hispid beetle, ultimately promoting development of efficient control policies. [ABSTRACT FROM AUTHOR]

Overwintering conditions affect the physiological state of ectotherms, and therefore, their cold hardiness and survival. A measure of the lethal and sublethal impacts of overwintering conditions on pest populations is crucial to predict population dynamics and to manage pests the following spring. The impact of winter conditions can be most intense for invasive insects undergoing range expansion. Insect herbivores can display plastic host use behaviours that depend on their body condition following winter. The pea leaf weevil, Sitona lineatus L. (Coleoptera: Curculionidae), is an invasive pest of field peas, Pisum sativum L., and faba bean, Vicia faba L. (Fabaceae). Pea leaf weevil has expanded its range in North America to include the Prairie Provinces of Canada. This study investigated the effects of temperature and microhabitat on overwintering survival and cold hardiness of pea leaf weevil in its expanded range. Further, we investigated the sublethal effect of overwintering temperature and duration on post‐overwintering survival, feeding, and oviposition of pea leaf weevil. We also investigated the role of juvenile hormone in modulating body condition of overwintering weevils. The overwintering survival of pea leaf weevil adults increased with soil temperature and varied with region and microhabitat. More weevils survived winters when positioned near tree shelterbelts compared to open alfalfa fields. The supercooling point of pea leaf weevil varied throughout its expanding range but did not differ for weevils held in the two microhabitats. The average threshold lethal temperature of pea leaf weevil at all three sites was −9.4 °C. Weevils that overwintered for a longer duration and at a higher temperature subsequently fed more on faba bean foliage and laid more eggs compared to those which overwintered for a shorter duration at a lower temperature. Our findings highlight that warm winters would increase overwintering survival and post‐overwintering fitness, facilitating further pea leaf weevil invasion northward in the Prairie Provinces of Canada. [ABSTRACT FROM AUTHOR]

Metamasius callizona (Chevrolat) (Coleoptera: Curculionidae) is an invasive weevil destroying Tillandsia utriculata L. (Bromeliaceae) populations in Florida, USA. A T. utriculata population in Belize coexists with M. callizona without adverse effects. Tillandsia utriculata and M. callizona from Belize and Florida were studied in the laboratory to determine how Belize T. utriculata can coexist with Belize M. callizona, using Florida and Belize T. utriculata, as well as pineapple, Ananas comosus (L.) Merr. (Bromeliaceae), as host plants. Florida M. callizona on pineapple leaves produced more eggs per female per day and had a shorter developmental time and pupation period, as well as a smaller adult width, than Belize M. callizona. Florida and Belize weevils did not differ significantly in rates of egg hatch, pupation, and adult emergence. Florida M. callizona laid similar numbers of eggs per day on Florida and Belize T. utriculata. Egg hatch rates were similar on pineapple and Florida and Belize T. utriculata. Pupation rates were similar on pineapple and Florida T. utriculata leaves; no larvae on Belize T. utriculata attained fourth instar. Field and laboratory observations revealed that Belize and Florida M. callizona mined Belize T. utriculata without destroying the meristem, allowing plants to survive, but Belize M. callizona destroyed the core of pineapple tops. Soluble solids were greatest in pineapple and least in Belize T. utriculata. Leaf toughness was greatest in pineapple and least in Florida T. utriculata. Metamasius callizona biology and behavior and T. utriculata plant characteristics may drive the severity of weevil damage on populations of T. utriculata. The discovery of a T. utriculata population in Belize coexisting with M. callizona offers a possible way to control M. callizona in Florida. [ABSTRACT FROM AUTHOR]

The increase in introduced insect pests and pathogens due to anthropogenic environmental changes has become a major concern for tree species worldwide. Common ash (Fraxinus excelsior L.) is one of such species facing a significant threat from the invasive fungal pathogen Hymenoscyphus fraxineus. Some studies have indicated that the susceptibility of ash to the pathogen is genetically determined, providing some hope for accelerated breeding programs that are aimed at increasing the resistance of ash populations. To address this challenge, we used a genomic selection strategy to identify potential genetic markers that are associated with resistance to the pathogen causing ash dieback. Through genome‐wide association studies (GWAS) of 300 common ash individuals from 30 populations across Poland (ddRAD, dataset A), we identified six significant SNP loci with a p‐value ≤1 × 10−4 associated with health status. To further evaluate the effectiveness of GWAS markers in predicting health status, we considered two genomic prediction scenarios. Firstly, we conducted cross‐validation on dataset A. Secondly, we trained markers on dataset A and tested them on dataset B, which involved whole‐genome sequencing of 20 individuals from two populations. Genomic prediction analysis revealed that the top SNPs identified via GWAS exhibited notably higher prediction accuracies compared to randomly selected SNPs, particularly with a larger number of SNPs. Cross‐validation analyses using dataset A showcased high genomic prediction accuracy, predicting tree health status with over 90% accuracy across the top SNP sets ranging from 500 to 10,000 SNPs from the GWAS datasets. However, no significant results emerged for health status when the model trained on dataset A was tested on dataset B. Our findings illuminate potential genetic markers associated with resistance to ash dieback, offering support for future breeding programs in Poland aimed at combating ash dieback and bolstering conservation efforts for this invaluable tree species. [ABSTRACT FROM AUTHOR]

Metcalfa pruinosa is a highly polyphagous, invasive insect in Europe with the capability of transferring phytoplasma. In the present study, we aim to identify volatile organic compounds that may act as semiochemicals for M. pruinosa. Using a dynamic volatile collection system, volatile organic compounds were collected from Ailanthus altissima, Aristolochia clematitis and Tagetes patula. Compounds were identified using gas chromatography–mass spectrometry. We also tested the electrophysiological responses of M. pruinosa to the collected volatile organic compounds using gas chromatography‐electroantennographic detection. In total, 77 volatile organic compounds were identified, and 29 compounds elicited an electroantennographic response. We analysed responses to volatile organic compounds extracted from Tagetes patula to compare antennal sensitivity between developmental stages and sexes. No differences were found in responders' number or response amplitude between males and females or adults and nymphs for any volatile. Based on the detected compounds, M. pruinosa adult females and males seem to have similar ecological needs in the host habitat. However, when we compared the response amplitudes among compounds, significant differences were found. These differences suggest that (E)‐4,8‐dimethyl‐nonatriene, methyl salicylate, and piperitone could have an important role in M. pruinosa's behaviour. This is the first contribution to M. pruinosa olfaction profile providing a base for future semiochemical‐based pest control tactics. [ABSTRACT FROM AUTHOR]

During the period 2019-2020, parasitoids of invasive oak lace bug (Corythucha arcuata) were studied in Bulgaria. Biological material (larvae of C. arcuata) was collected from European oak (Quercus robur) or Austrian oak (Quercus cerris) in five localities of the country. Five eulophid specimens (Hymenoptera: Eulophidae) were reared from the host larvae: Tamarixia pubescens, Elasmus sp., Minotetrastichus sp., Pnigalio sp. and Sympiesis sp. The impact of the parasitoids on the host number was very low (0.1-0.3%). The present records in Bulgaria are the first documented case of adaptation of native parasitoids to the host in the newly occupied areas in Europe and Asia. [ABSTRACT FROM AUTHOR]

Tuta absoluta (the tomato pinworm) is an invasive insect pest with a highly damaging effect on tomatoes causing between 80 and 100% yield losses if left uncontrolled. Resistance to chemical pesticides have been reported in some T. absoluta populations. Insect microbiome plays an important role in the behavior, physiology, and survivability of their host. In a bid to explore and develop an alternative control method, the associated microbiome of this insect was studied. In this study, we unraveled the bacterial biota of T. absoluta larvae and adults by sequencing and analyzing the 16S rRNA V3-V4 gene regions using Illumina NovaSeq PE250. Out of 2,092,015 amplicon sequence variants (ASVs) recovered from 30 samples (15 larvae and 15 adults), 1,268,810 and 823,205 ASVs were obtained from the larvae and adults, respectively. A total of 433 bacterial genera were shared between the adults and larval samples while 264 and 139 genera were unique to the larvae and adults, respectively. Amplicon metagenomic analyses of the sequences showed the dominance of the phylum Proteobacteria in the adult samples while Firmicutes and Proteobacteria dominated in the larval samples. Linear discriminant analysis effect size (LEfSe) comparison revealed the genera Pseudomonas, Delftia and Ralstonia to be differentially enriched in the adult samples while Enterococcus, Enterobacter, Lactococcus, Klebsiella and Wiessella were differentially abundant in the larvae. The diversity indices showed that the bacterial communities were not different between the insect samples collected from different geographical regions. However, the bacterial communities significantly differed based on the sample type between larvae and adults. A co-occurrence network of significantly correlated taxa revealed a strong interaction between the microbial communities. The functional analysis of the microbiome using FAPROTAX showed that denitrification, arsenite oxidation, methylotrophy and methanotrophy as the active functional groups of the adult and larvae microbiomes. Our results have revealed the core taxonomic, functional, and interacting microbiota of T. absoluta and these indicate that the larvae and adults harbor a similar but transitory set of bacteria. The results provide a novel insight and a basis for exploring microbiome-based biocontrol strategy for this invasive insect pest as well as the ecological significance of some of the identified microbiota is discussed. [ABSTRACT FROM AUTHOR]

The environmental impact of exotic insect pest species is often poorly quantified, making current pest management strategies obsolete. The fall armyworm (FAW) is endemic to the Western hemisphere – where it is considered a major pest of maize – and invasive to Africa, Asia and Oceania since 2016, 2018 and 2020, respectively. FAW's highly polyphagous behaviour and potential expansion of its host range in invaded habitats may put some non-crop flowering plants and their ecosystem services at risk. While its potential for intraguild competition and hybridisation with endemic maize pest species could have important consequences for pest population dynamics and pest management, a FAW inter-strain hybrid – resistant to pesticides – has already been documented in China. Integrated pest management (IPM) combines host plant resistance, agronomic practices and biological control for the long-term prevention of pests. Within invaded areas, a lack of knowledge of FAW's ecological adaptations, the absence of resistant cultivars, and an insufficient build-up of natural enemies provide challenges that translate into research opportunities to support IPM solutions for this exotic pest species. [ABSTRACT FROM AUTHOR]