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Wheat represents a fundamental component of the diet that contributes to ensuring global food security. However, its cultivation faces various abiotic and biotic constraints that result in fluctuating yields. The use of plant growth promoting rhizobacteria (PGPR) with the ability to solubilize phosphate presents itself as an environmentally friendly solution aimed to at enhancing sustainable agriculture practices. The objective of the present study is to assess the potential of PGPR in augmenting both yield and phosphorus (P) levels in wheat plants (Triticum durum L). under semi-controlled environmental conditions. Initially, the strains under investigation were screened for their capacity to dissolve different forms of mineral complex P, along with other plant growth promoting attributes such as iron chelation, Indole acetic acid (IAA) production, nitrogen fixation, and production of exopolysaccharides. Plant response to inoculation with PGPR was studied through an experiment conducted in a greenhouse involving four distinct treatments: (1) Control plants without any inoculation, (2) Inoculation with the PGP27 strain (Rahnella aquatilis), (3) Inoculation with the PGP25 strain (Erwinia persicina), and (4) Inoculation with the PGP374 strain (Raoultella terrigena). Results demonstrated that the studied strains exhibited significant promoting activities, including the solubilization of complex phosphate, the production of exopolysaccharides, IAA, siderophores, and atmospheric nitrogen fixation. Furthermore, the inoculation of plants with PGPR led to an increase in both biomass and plant phosphate concentrations, surpassing those observed in the control group. In conclusion, the findings propose that species like Raoultella and Erwinia have the potential to offer agronomic advantages as rhizobacteria that promote plant growth. [ABSTRACT FROM AUTHOR]

Efforts to raise awareness about foreign disinformation might accidentally increase distrust towards legitimate media. We argue that state discourse on disinformation is comparable to strategic framing in journalists' coverage of political events, and that it might imbue audiences with cynicism. Furthermore, in contrast to an experimental paradigm that depicts disinformation audiences as passive, we suggest that news consumers actively appropriate and produce content themselves. Conceptualising media content as 'strategic' rather than sincere might influence audiences to share and produce media content strategically. This Machiavellian tendency leads to similar effects on bias as motivated reasoning. Most accounts of motivated reasoning assume that limits of psychological processing are the reasons for biased judgements of what is true and fake, however, we argue that biases can also be due to culturally acquired second-order beliefs about knowledge. To explain this, we build on ideas about 'folk epistemology' and propose the term 'strategic epistemology towards media'. Resistance-building efforts against disinformation risk promoting such a strategic epistemology towards media and this can have harmful effects on democratic dialogue. To avoid this, educational interventions should be premised on social epistemology rather than experimental psychology. [ABSTRACT FROM AUTHOR]

• Inoculation increase the temperature, reduce the pH value and improve the GI. • Inoculants reduce the release of NH 3 and H 2 S. • Inoculants reduces the content of nitrogen compounds and increases the content of sulfur compounds. • There is correlation between physicochemical indexes and nitrogen and sulfur compounds in SS. In this study, high temperature thermotolerant nitrifying bacteria (TNB) and high temperature thermotolerant sulfide oxidizing bacteria (TSOB) were obtained from compost samples and inoculated into sewage sludge (SS) compost. The effects of inoculation on physical and chemical parameters, ammonia and hydrogen sulfide release, nitrogen form and sulfur compound content change and physical-chemical properties during nitrogen and sulfur conversion were studied. The results showed that inoculation of TNB and TSOB increased the temperature, pH, OM degradation, C/N ratio and germination index (GI) of compost. Compared with the control treatment (CK), the addition of inoculants reduced the release of NH 3 and H 2 S, and transformed them into nitrogen and sulfur compounds, the hydrolysis of polymeric ferrous sulfate was promoted, resulting in relatively high content of sulfite and sulfate. At the same time, the physical and chemical properties of SS have a strong correlation with nitrogen and sulfur compounds. [Display omitted] [ABSTRACT FROM AUTHOR]

Soil microbial mutualists like rhizobia bacteria can promote the establishment of rare, late‐successional legumes. Despite restoration efforts, these mutualists are often absent in the microbiome. Therefore, restoring this mutualism by directly inoculating rare legumes with rhizobia mutualists may increase plant establishment. We inoculated seedlings of Amorpha canescens, Dalea purpurea, and Lespedeza capitata with three strains of species‐specific rhizobia each to investigate how this mutualism would promote growth in the field and in the greenhouse. Because many herbaceous plants are vulnerable to herbivory, we used exclosures for half of our field transplantations to prevent mammalian herbivory. We did not find that rhizobia bacteria directly promoted the growth of our legumes in the field but rather that herbivory and environmental conditions overwhelmed the effects of the rhizobia. Of the plants transplanted, only 17.78% of 180 survived to the end of the growing season, all of which were protected from herbivory. Survival at the end of the growing season was also greater in the northern, drier end of the field site. In the second growing season, plants were more likely to survive in the exclosure treatment, while only four recovered in the open treatment. In the greenhouse, we found increased nodulation with inoculations, supporting the hypothesis that species‐specific mutualists are absent from restoration sites. Though several recent studies have shown that restoring mutualistic interactions has the potential to dramatically improve the outcomes of ecological restoration, our results show that protecting rare species from herbivory after transplantation might achieve greater gains in establishment. [ABSTRACT FROM AUTHOR]

Employing inoculation theory as a theoretical framework, we conducted an independent-groups experiment (N = 150), comparing the effects of an inoculation treatment to a control condition and examining uncertainty as a mediator of inoculation effects. To test inoculation effectiveness, we used an excerpt from an anti-vaccination conspiracy film Vaxxed as a counterattitudinal attack message. The results indicated that inoculation roused motivational defenses to protect initial attitudes and, subsequently, was effective at conferring resistance to conspiracy propaganda. Furthermore, relative to the control condition, inoculation treatment decreased attitudinal uncertainty. Importantly, and as predicted, within the inoculation condition, attitudinal uncertainty emerged as a mediator of the resistance process: For inoculated participants, an increase in motivational threat to defend one's attitudes fostered by the inoculation treatment reduced uncertainty, and attitudinal certainty, subsequently, facilitated resistance against the message advocated in the conspiracy film, damaging to public health. These results have theoretical implications for research on inoculation and offer practical import for attempts at mitigating conspiratorial ideation. [ABSTRACT FROM AUTHOR]

Theobroma cacao is primarily cultivated for its seeds. In the Philippines, where cacao yields are decreased by pod rots and vascular streak dieback (VSD)-like symptoms, the fungus Lasiodiplodia theobromae (Botryospaheriaceae) is the only reported species of Lasiodiplodia reported to cause disease in cacao. Here to identify and determine the pathogenicity of Lasiodiplodia isolates from cacao trees, we isolated Lasiodiplodia from from a leaf with blight (isolate PH22-080), stem with vascular streak (PH22-060), pod rot (PH22-120), an asymptomatic stem (PH22-014), and leaf litter (PH22-007). All strains were pathogenic on wounded leaves and stems and infected pods even without wounds. On the basis of molecular analysis of combined sequence data for ITS, rpb2, tef1-α and tub2 and morphological characteristics, the strains were identified as L. pseudotheobromae (PH22-080), L. theobromae (PH22-120), and Lasiodiplodia sp. (PH22-060), which were confirmed to be pathogenic on cacao leaves, stems, and pods. L. theobromae (PH22-007) and L. hormozganensis (PH22-014) showed potential to cause disease in cacao but further investigation is needed. [ABSTRACT FROM AUTHOR]

Introduction: This study tested the motivational power of inoculation to foster resistance to conspiracy propaganda (9/11 Truth Movement), comparing inoculation effects across United States and Finnish study participants. Method: We used a 2 inoculation (treatment vs. control) x 2 national culture (American vs. Finnish) independent groups design (N = 319), while examining the effects of motivational threat and thinking modes--analytic vs. intuitive--on the inoculation process. To test the effectiveness of the inoculation strategy, we used an excerpt from a conspiracy film Loose Change as a counterattitudinal attack message. Results: Our results indicated that inoculation was effective at motivating resistance regardless of national culture. Inoculation effects emerged mostly as a direct effect on resistance and two indirect effects wherein motivational threat mediated the relationship between inoculation and resistance as well as inoculation and analytic mode of message processing. Although we found that an increase in analytic mode of processing facilitated resistance and intuitive processing increased conspiracy-theory endorsement, the indirect effects between inoculation and resistance via message processing modes were not significant. Finally, the data revealed national culture differences in analytic mode and cultural-context differences mostly pertaining to the relationships between thinking styles, media literacy, and modes of thinking. Discussion: These results offer important theoretical implications for inoculation scholarship and suggest viable practical solutions for efforts to mitigate misinformation and conspiratorial beliefs. [ABSTRACT FROM AUTHOR]

Background: Endophytic fungi (EF) reside within plants without causing harm and provide benefits such as enhancing nutrients and producing bioactive compounds, which improve the medicinal properties of host plants. Selecting plants with established medicinal properties for studying EF is important, as it allows a deeper understanding of their influence. Therefore, the study aimed to investigate the impact of EF after inoculating the medicinal plant Perilla frutescens, specifically focusing on their role in enhancing medicinal properties. Results: In the current study, the impact of two EF i.e., Irpex lenis and Schizophyllum commune isolated from A. bracteosa was observed on plant Perilla frutescens leaves after inoculation. Plants were divided into four groups i.e., group A: the control group, group B: inoculated with I. lenis; group C: inoculated with S. commune and group D: inoculated with both the EF. Inoculation impact of I. lenis showed an increase in the concentration of chlorophyll a (5.32 mg/g), chlorophyll b (4.46 mg/g), total chlorophyll content (9.78 mg/g), protein (68.517 ± 0.77 mg/g), carbohydrates (137.886 ± 13.71 mg/g), and crude fiber (3.333 ± 0.37%). Furthermore, the plants inoculated with I. lenis showed the highest concentrations of P (14605 mg/kg), Mg (4964.320 mg/kg), Ca (27389.400 mg/kg), and Mn (86.883 mg/kg). The results of the phytochemical analysis also indicated an increased content of total flavonoids (2.347 mg/g), phenols (3.086 mg/g), tannins (3.902 mg/g), and alkaloids (1.037 mg/g) in the leaf extract of P. frutescens inoculated with I. lenis. Thus, overall the best results of inoculation were observed in Group B i.e. inoculated with I. lenis. GC-MS analysis of methanol leaf extract showed ten bioactive constituents, including 9-Octadecenoic acid (Z)-, methyl ester, and hexadecanoic acid, methyl ester as major constituents found in all the groups of P. frutescens leaves. The phenol (gallic acid) and flavonoids (rutin, kaempferol, and quercetin) were also observed to increase after inoculation by HPTLC analysis. The enhancement in the phytochemical content was co-related with improved anti-oxidant potential which was analyzed by DPPH (% Inhibition: 83.45 µg/ml) and FRAP (2.980 µM Fe (II) equivalent) assay as compared with the control group. Conclusion: Inoculation with I. lenis significantly enhances the uptake of nutritional constituents, phytochemicals, and antioxidant properties in P. frutescens, suggesting its potential to boost the therapeutic properties of host plants. [ABSTRACT FROM AUTHOR]

Misinformation is widely regarded as an undermining force to European democracies. Yet, to date, empirical research shows that the amount of misinformation people encounter is rather low, and not in proportion to the strong alarming messages spread throughout society. In this light, current interventions that pre-bunk misinformation by using warning messages may disproportionally prime suspicion and result in inflated estimates of misinformation. To assess whether messages that pre-bunk misinformation result in disproportionate risk perceptions related to inaccurate or false information, and to explore the effectiveness of alternative interventions, this article relied on an online between-subjects experiment in the Netherlands (N = 437). Our main findings indicate that exposure to a media literacy intervention does not result in higher first- or third-person risk perceptions related to misinformation exposure. However, a warning message that emphasizes the identification of reliable news while contextualizing the threats of misinformation significantly lowers perceived misinformation salience. As an important implication of our findings, we suggest that pre-bunking interventions should relativize the threats of misinformation by facilitating the recognition of honest and reliable information as an alternative path to help people identify reliable information. [ABSTRACT FROM AUTHOR]

Soil microbiota underpin ecosystem functionality yet are rarely targeted during ecosystem restoration. Soil microbiota recovery following native plant revegetation can take years to decades, while the effectiveness of soil inoculation treatments on microbiomes remains poorly explored. Therefore, innovative restoration treatments that target soil microbiota represent an opportunity to accelerate restoration outcomes. Here, we introduce the concept of ecological phage therapy—the application of phage for the targeted reduction of the most abundant and dominant bacterial taxa present in degraded ecosystems. We propose that naturally occurring bacteriophages—viruses that infect bacteria—could help rapidly shift soil microbiota towards target communities. Bacteriophages sculpt the microbiome by lysis of specific bacteria, and if followed by the addition of reference soil microbiota, such treatments could facilitate rapid reshaping of soil microbiota. Here, we experimentally tested this concept in a pilot study. We collected five replicate pre‐treatment degraded soil samples, then three replicate soil samples 48 hours after phage, bacteria, and control treatments. Bacterial 16S rDNA sequencing showed that phage‐treated soils had reduced bacterial diversity; however, when we combined ecological phage therapy with reference soil inoculation, we did not see a shift in soil bacterial community composition from degraded soil towards a reference‐like community. Our pilot study provides early evidence that ecological phage therapy could help accelerate the reshaping of soil microbiota with the ultimate aim of reducing timeframes for ecosystem recovery. We recommend the next steps for ecological phage therapy be (a) developing appropriate risk assessment and management frameworks, and (b) focussing research effort on its practical application to maximise its accessibility to restoration practitioners. [ABSTRACT FROM AUTHOR]

Teratosphaeria nubilosa is the predominant causal agent of Teratosphaeria leaf disease (TLD) in experimental plantations of E. globulus in Brazil. It exhibits slow vegetative growth and lack of sporulation in in vitro culture, making it difficult to obtain enough ascospores for mass inoculation. This study aimed to establish an inoculation method based on ascospore ejection from naturally infected leaves and use it to assess the response to T. nubilosa of eucalypt species and interspecific hybrids. First, the optimal temperature and incubation time for ascospores ejection from naturally infected E. globulus leaves were determined in vitro. Then, these optimal conditions were used to assess the response of eight eucalypt genotypes to T. nubilosa inoculation using ejected ascospores. The infected leaves were placed above (T1), above and under (T2) or under (T3) the plants to be inoculated. Significant differences in disease incidence, disease severity and plant defoliation were observed among genotypes and inoculation treatments. Plants exhibited higher incidence, more severe symptoms and more defoliation when exposed to T2. E. globulus and E. globulus × E. nitens clones were the most susceptible whereas an E. benthamii clone was immune. The sources of resistance identified in this work can be used in hybridization programmes with E. globulus to obtain interspecific hybrids expressing resistance to T. nubilosa and retaining the desirable traits for planting in temperate climates. The inoculation method optimized in this work could be used for small and medium‐scale screening of Eucalyptus spp. resistant to TLD. However, periodic validation of the species and genotype of the fungus associated with the naturally infected leaves used for inoculation and confirmation of the resistant phenotype of the selected eucalypt clones or progenies through experimental replications over time must be conducted. [ABSTRACT FROM AUTHOR]

San Mateo serves as the mungbean capital of the Philippines. However, it lacks information concerning indigenous microsymbiont that could be exploited to sustain mungbean production. This study aimed to evaluate the phylogeny and symbiotic potential of indigenous nodule bacteria in San Mateo, Philippines. A total of 183 bacterial strains were isolated from mungbean nodules established in the area. The isolates were grouped into 9 distinct morpho-biochemical clusters, coded as MB1–MB9. Thereafter, a representative isolate for each cluster was subjected to 16S rRNA gene sequencing. Phylogeny of the sequences revealed that the isolates are distributed to diverse bacterial genera namely Bradyrhizobium, Rhizobium, Pseudomonas, Bacillus, Agrobacterium, Stenotrophomonas and Leifsonia. Interestingly, both isolates MB1 (Bradyrhizobium) and MB7 (Rhizobium) induced nodulation in mungbean after authentication tests. Unlike previous reports, this study confirms that Rhizobium strains also harbors nodulating trait and establishes symbiotic relationship with mungbean in tropical soils. Furthermore, inoculation trial revealed that native strain MB1 and MB7 lead to a significant increase in leaf N-accumulation, nodulation and shoot yield with corresponding symbiotic scores of 94.32 and 39.70% respectively. These results suggest the remarkable symbiotic performance of local strains isolated, which could be utilized to further boost mungbean production in the municipality. [ABSTRACT FROM AUTHOR]

Purpose: to determine the effect of pre-sowing seed treatment and foliar application of drugs on the productivity and quality of the resulting soybean grain at various water availability under irrigation. The use of a biological preparation as an, inoculant which contributed to the growth of the necessary nodule bacteria on the root system of a given crop and, as a result, carried out the symbiosis of a legume plant, for the assimilation of nitrogen from the air and its conversion into a mineral compound in the soil. Materials and methods. Studies of this technique were conducted on light chestnut soils of the Volgograd region with a medium-ripened variety of own selection Volgogradka 2, bred by the All-Russian Research Institute of Irrigated Agriculture. The laying of experiments and related observations were carried out according to generally accepted methods for two years 2022–2023 on the lands of the All-Russian Research Institute of Irrigated Agriculture (experimental station “Irrigated”, Vodny settlement, Volgograd). Results. The obtained data prove the advantage of pre-sowing inoculation of seeds under different water regimes of this crop. At the same time, the increase in the yield of soybean grain, depending on such pre-sowing treatment, exceeded the control option by 0.40 t/ha under the water regime of 70-80-70 % the lowest moisture capacity and 0.54 t/ha not lower than 80% the lowest moisture capacity. Conclusions. In the conducted studies, the best option proved to be the option with a pre-watering soil moisture threshold of 80% the lowest moisture capacity in moisture layers of 0.4 and 0.6 m and pre-sowing inoculation of seeds in combination with leaf spraying with preparations of various origins, which contributed to a maximum yield of 3.91 t/ha and a profitability level of 242.10 %.

Abstract Background Endophytic fungi (EF) reside within plants without causing harm and provide benefits such as enhancing nutrients and producing bioactive compounds, which improve the medicinal properties of host plants. Selecting plants with established medicinal properties for studying EF is important, as it allows a deeper understanding of their influence. Therefore, the study aimed to investigate the impact of EF after inoculating the medicinal plant Perilla frutescens, specifically focusing on their role in enhancing medicinal properties. Results In the current study, the impact of two EF i.e., Irpex lenis and Schizophyllum commune isolated from A. bracteosa was observed on plant Perilla frutescens leaves after inoculation. Plants were divided into four groups i.e., group A: the control group, group B: inoculated with I. lenis; group C: inoculated with S. commune and group D: inoculated with both the EF. Inoculation impact of I. lenis showed an increase in the concentration of chlorophyll a (5.32 mg/g), chlorophyll b (4.46 mg/g), total chlorophyll content (9.78 mg/g), protein (68.517 ± 0.77 mg/g), carbohydrates (137.886 ± 13.71 mg/g), and crude fiber (3.333 ± 0.37%). Furthermore, the plants inoculated with I. lenis showed the highest concentrations of P (14605 mg/kg), Mg (4964.320 mg/kg), Ca (27389.400 mg/kg), and Mn (86.883 mg/kg). The results of the phytochemical analysis also indicated an increased content of total flavonoids (2.347 mg/g), phenols (3.086 mg/g), tannins (3.902 mg/g), and alkaloids (1.037 mg/g) in the leaf extract of P. frutescens inoculated with I. lenis. Thus, overall the best results of inoculation were observed in Group B i.e. inoculated with I. lenis. GC-MS analysis of methanol leaf extract showed ten bioactive constituents, including 9-Octadecenoic acid (Z)-, methyl ester, and hexadecanoic acid, methyl ester as major constituents found in all the groups of P. frutescens leaves. The phenol (gallic acid) and flavonoids (rutin, kaempferol, and quercetin) were also observed to increase after inoculation by HPTLC analysis. The enhancement in the phytochemical content was co-related with improved anti-oxidant potential which was analyzed by DPPH (% Inhibition: 83.45 µg/ml) and FRAP (2.980 µM Fe (II) equivalent) assay as compared with the control group. Conclusion Inoculation with I. lenis significantly enhances the uptake of nutritional constituents, phytochemicals, and antioxidant properties in P. frutescens, suggesting its potential to boost the therapeutic properties of host plants. Graphical Abstract

Relevance. Vegetable soybean is a special variety with high added value, consumed fresh and harvested at the stage technical ripeness (R6). Accession-A is one of the promising vegetable soybean materials, studied and identified as a result of work that was carried out jointly with the Federal Scientific Vegetable Center in the Moscow Region (FSVC) and the Agrobiotechnology Department of the Agricultural Technological Institute (ATI) of the RUDN University named after Patrice Lumumba. The use of microorganisms to promote atmospheric nitrogen fixation is a common practice in soybean production.Methodology. The purpose of this study was to evaluate the effect of two microbiological preparations based on Sinorhizobium fredii and Bradyrhizobium jaроniсum in order to identify which microbiological preparation gives the best results in terms of morphological indicators and vegetable soybean yield under the conditions region of the Non-Chernozem zone of the Russian Federation. The object of the study was accession-A of vegetable soybean. Two types of treatment were carried out: B1 – treatment with a preparation with Bradyrhizobium jaronicum (1 l/t of seeds), B2 – treatment with a preparation with Sinorhizobium fredii (1 l/t of seeds). Control without treatment (K).Results. According to statistical analysis of biometric indicators, it was found that treatment with S. fredii (B2) had the highest average values for indicators of stem diameter (11.50 mm), lower pod attachment height (11.19 cm), number of branches (3.40 pcs.) and the number of productive nodes (27.48 pcs.). Preparation B2 improved the structure of the crop, influencing the obtainment of the highest indicators of plant weight (30.47 g), seed weight per plant (15.33 g), weight of 1000 seeds (171.25 g), number of seeds per plant (98.50 pcs.), number of seeds in a bean (2.89 pcs.) and seed yield (3.11 t/ha). The use of microbiological preparations based on B. japonicum and S. fredii gave better results compared to the control treatment.

Desert biocrusts play an important role in the control of desertification and artificial inoculation can promote the formation and development of biocrusts. Physiological and growth responses of biocrusts inoculated on desert surfaces were investigated to assess the effect of mixture ratio, inoculation times, and water supply under laboratory conditions. The application of biological sand-fixing material prepared by cultivated algae crust and polymeric composites in a 1:1 ratio accelerated the most accumulation of chlorophyll a in 0.55 mg kg-1, thickness in 3.06 mm, and fresh mass in 0.69 g cm-1, was the most beneficial to formation and development of artificial biocrust. The water supply and cultivation time always significantly promoted the growth and accumulation of chlorophyll a and biomass under artificial cultivation and inoculation treatments. Artificial inoculation of biological sand-fixing material can lead to the formation of desert biocrust, which provides an engineering application method for desertification control.

Background. In order to reduce the negative impact of mineral fertilizers and pesticides on agroecosystems, environmentally friendly plant protection systems are increasingly being utilized in soybean cultivation technologies. These systems aim to provide essential nutrients to plants while minimizing ecological harm. Materials and Methods. In vegetative studies, the processes of formation and functioning of symbiotic systems in soybeans with active strains of nodulating bacteria Bradyrhizobium japonicum (РC07, PC09, B78, B144) were investigated under the influence of pre-sowing seed treatment with the biofungicides Mycosan-N and Phytocide-r. Microbiological, physiological, statistical methods, and gas chromatography were employed in the research. Results. The impact of biological fungicides on the nodulation activity of nodule bacteria has been identified. It was determined that with comprehensive treatment of soybean seeds with B. japonicum РC07 and Mycosan-N, the mass of nodules formed on the roots exceeded that of the control plants by 7‒22 %, and under the influence of Phytocide-r, the mass was lower by 6‒20 % throughout the vegetation period. With the combined application of B. japonicum PC09 and Mycosan-N, a decrease in the number of root nodules by 8‒25 % and a reduction in their mass by 18‒35 % was observed throughout the vegetation period. Under the influence of Phytocide-r, with the inoculation of rhizobia strain PC09, the number of nodules was lower compared to the control plants only in the full flowering stage. At the same time, the reduction in their mass was 6‒20 % throughout the entire observation period. A stimulating effect of both biofungicides on the formation of the symbiotic apparatus involving strains B. japonicum B78 and B144 has been identified. With the combined application of B. japonicum РC07 and Phytocide-r, nitrogenase activity of symbiotic systems decreased by 7 % only in the stage of three true leaves compared to plants whose seeds were treated only with rhizobia. The nitrogenase activity of symbiotic systems formed with the participation of B. japonicum B78 and under the influence of Mycosan-N increased by 28 %, 15 %, and 12 % in the stages of three true leaves, budding, and full flowering, respectively. The action of Phytocide-r, with the inoculation by the nodule bacteria B78, resulted in an increase in nitrogenase activity by 14 % only in the budding stage. Under the comprehensive treatment of soybean seeds with involved biofungicides and B. japonicum B144, an increase in the intensity of N2 assimilation was noted by 29‒34 % in the stage of three true leaves and by 10‒16 % in the budding stage. Conclusions. The application of a scientifically justified selection of rhizobial strains and biological plant protection agents for pre-sowing treatment of soybean seeds will enable a more complete realization of their nitrogen-fixing potential and phytoprotective effects.

Field peas (Pisum sativum L.) is economically a significant crop in Ethiopia. The yield of field peas in our country has fell due to poor management practices of Bio fertilizer rates usage. Three rates of bio fertilizer (0, 250 and 500g ha-1) were laid out in Randomized Complete Block Design with three replications. Number of nodules per plant, Pod numbers per plant, plant height, and effective nodule number of productive tillers, total biomass yield, grain yield, and straw yield were recorded and significantly affected by bio-fertilizer rate application. The determined or the highest grain yield (2671kg ha-1) was recorded from 250 g ha-1 bio-fertilizer rate. Almost 250g ha-1 bio fertilizer rates were superior for the research area. Nevertheless, more investigation has to be done under different locations and seasons to come up with practical recommendations correlated to the current study.

Shallots are essential vegetables in horticulture, but there is insufficient information available on the effects of drought stress on different growth stages and the inoculation of plant growth-promoting rhizobacteria (PGPR) on their photosynthetic characteristics. This study aims to investigate the effects of drought stress at different growth stages (vegetative growth phase, bulb initiation phase, bulb development phase, and maturation phase) and PGPR inoculation (Pseudomonas Pb04 and Bacillus Pb03) to mitigate the negative impact of drought stress on photosynthetic characteristics, chlorophyll content, and shallot yield. The results showed that the optimal photosynthesis rate, chlorophyll content, and yield of shallots were most tolerant when the plants experienced drought stress in the maturation phase compared to other growth phases. During the maturation phase, the reduction in photosynthesis rate at PPFD 900 µmol.m-2.s-1 is only 19.1% compared to plants without drought stress. Drought stress during the bulb growth phase takes the longest to recover conditions after stress, leading to inhibited growth when stress occurs during this phase. In the bulb growth phase, the decrease in photosynthesis rate is 34.8% compared to the treatment without drought stress. PGPR can mitigate the sensitivity of plants to drought stress. Pseudomonas Pb04 predominantly suppresses the impact of drought stress during the vegetative growth phase, while Bacillus Pb03 has a more dominant effect on drought stress occurring during bulb initiation, bulb development, and maturation phases.

Mining-related lead (Pb) pollution of the soil poses serious hazards to ecosystems and living organisms, including humans. Improved heavy metal phytoremediation efficacy, achieved by using phytostabilizing plants assisted by plant-growth-promoting (PGP) microorganisms, has been presented as an effective strategy for remediating polluted soils. The objective of this research was to examine the response and potential of the plant-growth-promoting bacterium LMR356, a Rhodococcus qingshengii strain isolated from an abandoned mining soil, under lead stress conditions. Compared to non-contaminated culture media, the presence of lead induced a significant decrease in auxin production (from 21.17 to 2.65 μg mL−1) and phosphate solubilization (from 33.60 to 8.22 mg L−1), whereas other PGP traits increased drastically, such as 1-aminocyclopropane-1-carboxylic acid (ACC) deaminase activity (from 38.17 to 71.37 nmol mg−1 h−1 α-ketobutyrate), siderophore production (from 69 to 83%), exopolysaccharide production (from 1952.28 to 3637.72 mg mL−1), biofilm formation, and motility. We, therefore, investigated the behavior of Sulla spinosissima L. in the presence or absence of this strain under a variety of experimental conditions. Under hydroponic conditions, Sulla plants showed endurance to varying lead concentrations (500–1000 μM). Inoculation of plants with Rhodococcus qingshengii strain LMR356 enhanced plant tolerance, as demonstrated by the increase in plant biomass (ranging from 14.41 to 79.12%) compared to non-inoculated Pb-stressed and non-stressed control plants. Antioxidant enzyme activities (increasing by −42.71 to 126.8%) and chlorophyll (383.33%) and carotenoid (613.04%) content were also augmented. In addition to its impact on plant lead tolerance, strain LMR356 showed a growth-promoting effect on Sulla plants when cultivated in sterilized non-contaminated sand. Parameters such as plant biomass (16.57%), chlorophyll (24.14%), and carotenoid (30%) contents, as well as ascorbate peroxidase (APX), peroxidase (POD), and catalase (CAT) activities, were all elevated compared to non-inoculated plants. Furthermore, when the same plant species was cultivated in highly polluted soil, inoculation increased plant biomass and improved its physiological properties. These findings demonstrate that LMR356 is a phytobeneficial bacterial strain capable of enhancing Sulla growth under normal conditions and improving its heavy metal tolerance in multi-polluted soils. Thus, it can be considered a promising biofertilizer candidate for growing Sulla spinosissima L. or other selected plants intended for application in restoration and stabilization initiatives aimed at reviving and safeguarding environmentally compromised and polluted soils after mining activities. [ABSTRACT FROM AUTHOR]

Carrot is a major root vegetable crop grown in many parts of the world. In Europe, cavity spot disease continues to have a major impact on marketable yield. The disease is caused by several Pythium spp., with P. violae the main pathogen in the UK and results in small black lesions on mature carrot roots. The lack of reliable inoculation methods for these Pythium pathogens has, for many years, hampered the identification of new effective crop protection products or carrot varieties that are resistant to the disease. In this research, inoculation methods were developed for P. violae using mycelium or oospores, each of which successfully induced typical cavity spot symptoms in both pot‐ and field‐grown carrots as well as consistent root stunting in the former. These methods were also used to successfully identify carrot cultivars with resistance to cavity spot and confirmed the efficacy of the fungicide metalaxyl‐M against the disease. Results therefore demonstrated that the inoculation methods should be reliable for identifying the efficacy of crop protection products, assessing cavity spot resistance and for further studies investigating the biology and epidemiology of the pathogen. [ABSTRACT FROM AUTHOR]

In Ethiopia, resistant varieties and cultural disease management methods are commonly used to combat Coffee Berry Disease. However, the lack of improved and resistant varieties in coffee-growing areas, including the Arsi Zone, remains a challenge. The main objective of this study was to assess the resistance of Arsi coffee collections to coffee Berry Disease through artificial inoculation of green berries on mother trees and younger coffee seedlings under controlled conditions. The research was conducted at Gololcha (Arbagugu state farm) and Mechara Agricultural Research Center. Additionally, a hypocotyl test was performed at the Jimma Agricultural Research Center. For the field experiment, 13 Arsi coffee collections and the coffee Berry Disease -susceptible Arusa coffee variety were tested. Standard checks like the susceptible variety (370) and resistant varieties 741 and 754 were used for the hypocotyl tests, with a total of seventeen treatments. The results demonstrated a significant difference in coffee Berry Disease reaction levels among the different genotypes under field conditions (P < 0.001). The infection percentage of berries varied from 2.89% to 25.93% at Gololcha and 3.25% to 34.92% at Mechara three weeks after the treatment applications. Notably, the resistant variety 741 exhibited the lowest infection percentage (15.04%) compared to all other collections and varieties at the seedling stage. Given these findings, the coffee collections that displayed the lowest infection percentages under both experimental conditions (Ar15/11, Ar05/11, and Ar70/11) were recommended for further breeding strategies aimed at developing resistant coffee varieties against coffee Berry Disease. [ABSTRACT FROM AUTHOR]

The effect of inoculants was confirmed to improve the mass effect of ferritic spheroidal graphite iron castings with heavy sections. In the first series of tests, primary inoculation was performed simultaneously with the spheroidizing treatment, followed by the addition of post-inoculant, and the effectiveness of this method was evaluated in the terms of nodule count and mechanical properties. Post-inoculation is the common term for inoculation after initial treatment. In the first series of tests, six inoculants were evaluated in separately cast test samples, for the second part of the evaluation, Fe–Si–Ca–Zr and Fe–Si–Ca–Ba① were selected. In the second series of tests, these inoculants were used and evaluated by cast on test samples with the wall thickness of 40 and 70 mm. The results showed that possibly the change to Fe–Si–Ca–Zr provided the greatest increase in the nodule count. Tensile properties of samples post-inoculated Fe–Si–Ca–Zr showed almost the same values for tensile strength, proof stress, and elongation at 40 mm and 70 mm wall thickness. In impact tests, differences in absorbed energy were observed in samples with a wall thickness of 70 mm, with Fe–Si–Ca–Zr having the highest absorbed energy. Therefore, the Fe–Si–Ca–Zr inoculant is effective post-inoculation for reducing mass effect. Also, the factors were discussed through electron probe micro analyzer (EPMA) analysis of inoculant. [ABSTRACT FROM AUTHOR]

The selection of MgFeSi alloy and inoculants used for ductile iron treatment has confounded members of the foundry industry for many years. Frequently foundries have used certain types of alloys "because they have always done it that way" or "because someone else was doing it." The current study involves ductile iron produced with three grades of MgFeSi with varying levels and types of rare earths along with four different inoculants. The iron was cast into test samples of various thickness from 2×2×8 inches to 10×10×8 inches. Mechanical properties and microstructures were evaluated at multiple locations in each of the 36 samples for a total of 156 points of data. In this paper, we will summarize the findings for differing section sizes. [ABSTRACT FROM AUTHOR]

Data-driven political campaigning strategies often remain a black box for citizens; however, educational interventions provide a means to enhance understanding, conscious evaluations, and skills. In this context, we term this combination digital campaign competence (DCC). We conducted an online pre-registered experiment in Austria (N = 553) using a 2 × 2 between-subject design to compare intervention formats (reading a voter guide vs. playing a campaign game) and content framing (emphasizing risks vs. benefits of data-driven campaigning) plus a control condition. Results show no significant differences in framing on DCC. However, variations are observed among different formats, with the non-interactive voter guide proving to be the most effective one. Contrary to our expectations, the voter guide emphasizing the risks of data-driven political campaigning enhanced conceptual understanding levels, influenced evaluative perceptions, and aided skill development to detect highly targeted ads. We argue that innovative interventions do not always guarantee success in enhancing competencies. [ABSTRACT FROM AUTHOR]

Co-inoculation of soybeans with Bradyrhizobium and plant growth-promoting bacteria has displayed promise for enhancing plant growth, but concrete evidence of its impact on soybean yields is limited. Therefore, this study assessed the comparative efficacy of two 1-aminocyclopropane-1-carboxylate deaminase-producing Pseudomonas species (OFT2 and OFT5) co-inoculated with Bradyrhizobium ottawaense (SG09) on the growth, physiology, nodulation efficiency, and grain yield of three major Japanese soybean cultivars: Enrei, Fukuyutaka, and Satonohohoemi. The experiments were conducted in a warehouse under natural light conditions. The treatments included the inoculation of SG09, SG09 + OFT2, and SG09 + OFT5. Compared with Bradyrhizobium inoculation alone, co-inoculation led to significant improvements in nodulation efficiency, growth, and physiological performance in the Enrei and Fukuyutaka cultivars, but not in the Satonohohoemi cultivar. Furthermore, co-inoculation significantly boosted the total nitrogen content and ion uptake in the shoots, ultimately leading to a remarkable improvement in the grain yield in the Enrei and Fukuyutaka cultivars. These findings contribute to clarifying the interplay among Bradyrhizobium, Pseudomonas, and the plant host cultivar. Notably, Bradyrhizobium–Pseudomonas co-inoculation represents a potentially effective biofertilization strategy for soybean production, highlighting promising avenues for sustainable agricultural practices. [ABSTRACT FROM AUTHOR]

Seed coating is an effective method for delivering beneficial microorganisms to the soil. Additionally, coated seeds are protected against pathogens or microbial contaminants during storage. This work aimed to evaluate the effect of seed coating with the beneficial fungus Trichoderma koningiopsis against fungal contaminants of sweet sorghum (Sorghum bicolor (L.) Moench) during storage. Sorghum-treated seeds with three concentrations of Trichoderma koningiopsis were stored at two temperatures (5 °C and 18 °C) over 12 months to evaluate physiological-, and sanitary quality of seeds. The concentrations of the most prevalent contaminant fungi Diaporthe melonis, Curvularia lunata and Penicillium polinocum were monitored. The viability of Trichoderma koningiopsis was evaluated every three months and correlated with the seed quality variables. The results indicate that beneficial fungi can protect seeds against fungal contamination during storage avoiding the application of a chemical fungicide. The coating procedure did not affect the seed germination over the 12 months of storage. [ABSTRACT FROM AUTHOR]

The effect of an inoculum composed of Amanita stranella and Suillus decipiens was evaluated on morphological features and total phenolic compounds in seedlings of Pinus pseudostrobus var. coatepecensis – a narrow endemic Mexican variety. The treatments were used: (1) A. stranella; (2) S. decipiens; (3) combined inoculum and (4) control, using a completely randomized design with 12 seedlings per treatment. The morphotype descriptions of both fungi significantly facilitate their identification in the field. The results showed significant differences in plant growth among the treatment groups. Seedlings treated with the combined treatment exhibited remarkable increases in height, surpassing the A. stranella, S. decipiens treatments, and the control group by 21.81%, 28.83%, and 59%, respectively. Moreover, the root collar diameter in the combined treatment group measured 2.53 cm, contrasting with the control group, 1.05 cm. Additionally, the shoot height/root length ratio and Dickson's index were both close to 1 in the combined treatment, suggesting a well-developed root system in relation to the above-ground part of the plant. The percentage of ectomycorrhizal root colonization showed no significant differences among the inoculated treatments. Furthermore, the combined treatment significantly increased the total phenolic content in plants (39.2 mg GAE g− 1). Therefore, the combined inoculation of P. pseudostrobus var. coatepecensis seedlings with A. stranella and S. decipiens is recommended during the nursery stage for reforestation practices. [ABSTRACT FROM AUTHOR]

Seed inoculation with symbiotic bacteria is a commonly employed practice in soybean cultivation. As a result, nodulation proceeds properly and plants self-supply atmospheric nitrogen, requiring either minimal or no additional nitrogen fertilization. The aim of the study was to investigate the response of soybeans to the application of the recommended or double dose of commercial inoculants (HiStick® Soy or TURBOSOY®) and/or mineral nitrogen fertilization compared to the untreated control. It was demonstrated that a double dose of the tested preparations had the most favorable effect on nodulation. However, the impact of weather conditions modified their effectiveness during the study years, which was especially visible in 2022. Sowing seeds without inoculation (control) resulted in the formation of sparse root nodules and consequently the lowest leaf area index (LAI) and soil plant analysis development (SPAD) measurements. In addition, the values of SPAD and LAI indices varied across the years of the study, indicating that weather conditions modified nitrogen uptake by plants. Overall, seed inoculation and/or nitrogen fertilization positively influenced the chemical composition of seeds compared to the control. The only decrease observed was in the oil content, while the double dose of HiStick® Soy preparation reduced the polyphenol content. The double dose of the tested inoculants had the most favorable impact on yield components and seed yield. However, applying inoculation at the recommended dose or in combination with nitrogen fertilization yielded similar or slightly worse results, depending on the year. Therefore, soybean seed inoculation should be recommended, although the effectiveness of the procedure will depend on various factors, including the type of inoculant, dosage, nitrogen fertilization, and weather conditions. [ABSTRACT FROM AUTHOR]

The corrosion resistance of bearing materials is crucial for the long-term service and high precision of modern equipment, and has aroused widespread research interest. Inoculation treatment is beneficial for improving the mechanical properties of GCr15 bearing steel, but its impact on corrosion behavior remains to be investigated. In the present work, the influence of inoculation treatment on the corrosion morphology, open circuit potential, impedance spectrum, and polarization curve evolution of GCr15 steel was studied through in situ corrosion morphology analysis and electrochemical testing. The results showed that the samples treated after inoculation showed a reduced tendency for pitting corrosion and an obviously improved corrosion resistance. This improvement is related to the transformation of columnar grains into equiaxed grains during the inoculation process, which reduces the amount and distribution of inclusions and pores, thereby delaying the rapid development of pitting corrosion. This study provides new insights into the corrosion mechanism of gestational steel. [ABSTRACT FROM AUTHOR]

Biogas digestive effluent (BDE) is a nutrient-enriched source that can be utilized as an organic fertilizer for rice cultivation without synthetic fertilizer (SF) application. However, a primary concern is the stimulation of methane (CH4) emissions due to the enrichment of the labile organic carbon, a favorite substrate of methanogenic archaea. Methanotrophs potentially reduce greenhouse gas (GHG) emissions from rice fields owing to metabolizing CH4 as a carbon source and energy. We therefore examined the effect of the application of methanotroph-inoculated BDE to the rice cultivated paddy soil on GHG emissions and rice productivity under a pot experiment. Methanotrophs (Methylosinus sp. and Methylocystis sp.), isolated from the Vietnamese Mekong Delta's rice fields, were separately inoculated to the heated BDE, followed by a 5-day preincubation. Methanotroph-inoculated BDE was supplied to rice cultivation to substitute SF at 50% or 100% in terms of nitrogen amount. The results showed that the total CH4 emissions increased ~34% with the application of BDE. CH4 emissions were significantly reduced by ~17–21% and ~28–44% under the application of methanotroph-inoculated BDE at 100% and 50%, respectively. The reduction in CH4 was commensurate with the augmentation of pmoA transcript copy number under methanotroph-inoculated BDE. In addition, methanotroph-inoculated BDE application did not increase nitrous oxide (N2O) emissions and adversely affect rice growth and grain productivity. This study highlighted the BDE-recirculated feasibility for a lower CH4 emission rice production based on methanotrophs where high CH4-emitting fields were confirmed. [ABSTRACT FROM AUTHOR]

This experimental study evaluated the effectiveness of different essential oils against the in vitro growth of Sclerotium rolfsii. The experiment employed a completely randomized design (CRD) with three concentrations (500, 1000, and 1500 ppm) of each essential oil, including thyme oil (Thymus vulgaris L.), cinnamon oil (Cinnamomum zeylanicum Blume), juniper oil (Juniperus horizontalis L.), neem oil (Azadirachta indica A. Juss.), lemon grass oil (Cymbopogon citratus (DC.) Stapf), peppermint oil (Mentha piperita L.), and an unamended control medium. This setup aimed to evaluate their efficacy against the mycelial growth of S. rolfsii. The data were analyzed using R software in R-Studio, and means were compared using Duncan's Multiple Range Test (DMRT) at a 5% level of significance. Mycelium growth data were recorded at 24 hours, 48 hours, and 72 hours of incubation. All tested essential oils significantly inhibited the mycelial growth of the pathogen compared to the control (p<0.05). After 72 hours, thyme oil at all concentrations and lemongrass oil at 1500 ppm both achieved 100% growth inhibition. In contrast, neem oil at 500 and 1000 ppm showed the lowest inhibitory effects, with rates of 27.56% and 34.62%, respectively. Lemongrass oil at 500 ppm (75.39%) showed statistical similarity to cinnamon oil at 1000 ppm (79.12%). Peppermint oil at 1500 ppm resulted in 82.73% inhibition, and cinnamon oil at 1000 ppm (75.73%) showed comparable results to peppermint oil at 1000 ppm. Thus, the study highlights the superior performance of thyme oil among the tested essential oils. These effective essential oils can potentially be used at lower concentrations to minimize potential hazards. However, further research and field trials are essential to validate these findings for practical applications. [ABSTRACT FROM AUTHOR]

In the aftermath of an insurrection, the role of propaganda garners more attention as Americans begin to confront the destabilizing and radicalizing effects that a sustained propaganda campaign can have on an anxious public. Given the potential for an even greater resurgence in the use of propaganda, this study examined an inoculation-based treatment designed to increase propaganda education in just ninety minutes. The treatment targets the use of common propaganda techniques in contemporary political rhetoric by exposing the message receiver to instances of these techniques in authentic use. The treatment is designed to elicit motivation and prompt analytical skill building aimed at increasing one's ability to detect the usage of propaganda techniques. The results reveal a large effect size in propaganda detection accuracy for participants. In addition, the findings point to a robust effect unimpacted by the frequency of visits to the training site or the length of time spent in training beyond the ninety minutes, thus providing an impetus for follow-up research. [ABSTRACT FROM AUTHOR]

Summary: Communities of arbuscular mycorrhizal (AM) fungi assemble passively over time via biotic and abiotic mechanisms. In degraded soils, AM fungal communities can assemble actively when humans manage mycorrhizas for ecosystem restoration.We investigated mechanisms of urban AM fungal community assembly in a 2‐yr green roof experiment. We compared AM fungal communities in inoculated and uninoculated trays to samples from two potential sources: the inoculum and air.Active inoculation stimulated more distinct and diverse AM fungal communities, an effect that intensified over time. In the treatment trays, 45% of AM fungal taxa were detected in the inoculum, 2% were detected in aerial samples, 23% were detected in both inoculum and air, and 30% were not detected in either source.Passive dispersal of AM fungi likely resulted in the successful establishment of a small number of species, but active inoculation with native AM fungal species resulted in an immediate shift to a diverse and unique fungal community. When urban soils are constructed or modified by human activity, this is an opportunity for intervention with AM fungi that will persist and add diversity to that system. [ABSTRACT FROM AUTHOR]

A significant legume crop in Ethiopia, chickpeas (Cicer arietinum L.) have several advantages, including high nutritional value and the capacity to improve soils deficient in nitrogen through biological nitrogen fixation using several endosymbiotic Mesorhizobium spp. strains. However, the host variety, the soil's capacity to hold nutrients, and the endosymbiont's innate physiological traits all affect how efficient the strains are. The primary obstacles to its cultivation in the research area are inadequate agronomicmethods and low soil fertility [low nitrogen (N), low soil organic matter (OM), low accessible phosphorous (P), sulfur (S), and boron (B)], which results in ineffective nodulation. To evaluate the effects of NPSB fertilization and inoculation, a field experiment was carried out in Buchach Kebele's Cheha area during the primary cropping season of 2021/22. The trial included two chickpea kinds (Local and Arerti), twoNPSB levels (zero and 121 kg NPSB ha-1), and four levels of Mesorhizobium strains (CP-M41, CP-EAL 029, CP-M20b, and un-inoculated control). A randomized complete block design (RCBD) was used to organize the treatments in a factorial form with three replications. In comparison to the single application and the control, the interaction impact of strains, NPSB fertilizer, and variety greatly increased nodulation parameters, growth parameters, yield, and yield components. The Arerti variety combined with the CP-M41 Mesorhizobium strain andNPSB fertilizer had themaximum grain production (3177.16 kg ha-1). It yielded 15.96%, 24.06%, and 37.93%more than the Arerti with CP-M41 strain, Arertiwith NPSB, and the control treatments, respectively. The partial budget analysis of the study treatments showed that the Arerti varietywith the combined application of 121 kg NPSB ha-1 and Mesorhizobium strain CP-M41 inoculation produced the highest net return (102,092.6 ETB ha-1) with an acceptable marginal rate of return (618%). It has been found that the CPM41 strain and the Arerti variety, when combined with 121 kg NPSB ha-1 application, is a suitable treatment combination to achieve increased chickpea crop yield and profit in the studied area. However, the results need further validation in the farmer's field before recommending to farmers. [ABSTRACT FROM AUTHOR]

The inoculation of nano-sized particles of refractory Al2O3, TiO2, MgO, ZrO2 oxides into the welding bath was studied. The inoculation of oxides with a low-level mismatch to the δ-Fe lattice and increased wetting with liquid iron (MgO, ZrO2) contributes to the growth of dendrites, which are formed during melt crystallization. Metal modification with MgO, ZrO2 oxides decreases the hardness of polygonal ferrite and increases the content of lower bainite in the microstructure of joints, brittle fracture resistance of the joint metal. Metal modification with Al2O3, TiO2 oxides contributes to an increase in the hardness of polygonal ferrite, the content of upper bainite in the joint microstructure, and the strength of welded joints of low-alloy steels. [ABSTRACT FROM AUTHOR]

Background: Little is known about research in Foundling Hospitals during the 18th century. Summary: The London "Hospital for the Maintenance and Education of Exposed and Deserted Young Children" opened in 1741, after fundraising by the former shipmaster Thomas Coram and a Charter by King George II. From 1741 to 1756, fewer than 100 infants a year were admitted by lot. With onset of the Seven Years' War in 1756, the House of Commons resolved and financed the admission of all deserted babies. The number of admitted babies rose to 4,000 per year, and their mortality increased. The Institution was not intended as a research and teaching facility but soon became a site for gaining knowledge of young infants. Appointed physicians included Richard Conyers, William Cadogan, William Watson, and William Buchan. Their research focused on frequent conditions in the hospital's infirmary such as scabies, fever, measles, chilblains and scorbutic eruptions, and set standards for infant care and nutrition in the English-speaking world during the 18th century. They described the dangers connected with tight swaddling, meconium purgation, artificial feeding, and the difficulty to obtain wet nurses in the big cities. A major topic was their fight against smallpox, then fatal for 80% if infected infants, and the development of an effective technique of inoculation. Key Messages: Research at the London Foundling Hospital differed from modern understanding of controlled clinical trials but revealed systematic, hypothesis-driven approaches in the mid-18th century. As in other Foundling Hospitals, absent parental interference facilitated innovations. [ABSTRACT FROM AUTHOR]

Field peas (Pisum sativum L.) is economically a significant crop in Ethiopia. The yield of field peas in our country has fell due to poor management practices of Bio fertilizer rates usage. Three rates of bio fertilizer (0, 250 and 500g ha-1) were laid out in Randomized Complete Block Design with three replications. Number of nodules per plant, Pod numbers per plant, plant height, and effective nodule number of productive tillers, total biomass yield, grain yield, and straw yield were recorded and significantly affected by bio-fertilizer rate application. The determined or the highest grain yield (2671kg ha-1) was recorded from 250 g ha-1 bio-fertilizer rate. Almost 250g ha-1 bio fertilizer rates were superior for the research area. Nevertheless, more investigation has to be done under different locations and seasons to come up with practical recommendations correlated to the current study. [ABSTRACT FROM AUTHOR]

Shallots are essential vegetables in horticulture, but there is insufficient information available on the effects of drought stress on different growth stages and the inoculation of plant growth-promoting rhizobacteria (PGPR) on their photosynthetic characteristics. This study aims to investigate the effects of drought stress at different growth stages (vegetative growth phase, bulb initiation phase, bulb development phase, and maturation phase) and PGPR inoculation (Pseudomonas Pb04 and Bacillus Pb03) to mitigate the negative impact of drought stress on photosynthetic characteristics, chlorophyll content, and shallot yield. The results showed that the optimal photosynthesis rate, chlorophyll content, and yield of shallots were most tolerant when the plants experienced drought stress in the maturation phase compared to other growth phases. During the maturation phase, the reduction in photosynthesis rate at PPFD 900 µmol·m-2s-1 is only 19.1% compared to plants without drought stress. Drought stress during the bulb growth phase takes the longest to recover conditions after stress, leading to inhibited growth when stress occurs during this phase. In the bulb growth phase, the decrease in photosynthesis rate is 34.8% compared to the treatment without drought stress. PGPR can mitigate the sensitivity of plants to drought stress. Pseudomonas Pb04 predominantly suppresses the impact of drought stress during the vegetative growth phase, while Bacillus Pb03 has a more dominant effect on drought stress occurring during bulb initiation, bulb development, and maturation phases. [ABSTRACT FROM AUTHOR]

The mycorrhizal association represents a crucial symbiotic bond necessary for the vitality of the majority of plants. This report aims to investigate the impact of endomycorrhizal fungi on the production of fig trees from cuttings. The research took place in a nursery environment by inoculating fig cuttings sourced from three sites in Morocco with a composite inoculum of arbuscular mycorrhizal fungi (AMF). The mean root and vegetative masses of the inoculated plants after 140 days were 33.1 g and 35 g compared with 1.8 g and 6.4 g in the control. The mean shoot length and number of leaves developed from treated cutting were 98.5 cm and 17, respectively 29 cm and 4 leaves in control. Thus, the newly formed roots showed a well-established mycorrhizal colonization and contained different structures characteristic of arbuscular endomycorrhizae: arbuscules, vesicles, endophytes, hyphae and spores. The frequency and intensity of root mycorrhization were approximately 80% and 15.5%, respectively. The contents of arbuscules and vesicles were 2.5% and 5.5%. The study of formed spores showed a combination of 52 spores per 100 g of soil, belonging to 11 species and 3 genera: Glomus, Acaulospora and Rhizophagus with dominance of the genus Glomus (85%). Glomus macrocarpum and Glomus deserticola were the most abundant species, appearing at 30.77% and 19.23%, respectively. The results demonstrate a pronounced stimulating effect on both root formation and vegetative shoot development in the inoculated cuttings. Specifically, the early inoculation of fig cutting with the AMF inoculum significantly enhanced rhizogenesis end root system development. Consequently, the gradual establishment of mycorrhizal symbiosis at the root level of inoculated plants led to good development of the root mass and the vegetative mass. The inoculated plants showed good root and vegetative mass development due to the installation and the progressive development of mycorrhizal symbiosis at their roots.

Drought and salinity are significant challenges to global food security. This study investigated the interactive impacts of Piriformospora indica inoculation with salinity and drought stresses on rice. Two greenhouse experiments were conducted. The first experiment evaluated two P. indica inoculation levels and three salinity levels (0-, 50-, and 100-mM sodium chloride), while the subsequent experiment assessed two inoculation levels under three drought intensities (25%, 50%, and 100% of available water content). P. indica spores were inoculated following optimized seed disinfection and germination processes. The shoot and root biomass under salinity stress were consistently higher in inoculated plants compared to controls. Sodium concentrations in shoots and roots exhibited an overall upward trend, with the trend being less pronounced in inoculated plants due to increased potassium uptake. Under salinity stress, nitrogen, magnesium, and calcium concentrations significantly increased in inoculated plants. With increasing salinity, there was a significant increase in catalase enzyme activity and soluble carbohydrate concentrations across all treatments, with a greater increase in inoculated plants. Plants under drought stress experienced reduced root and shoot biomass, but inoculated plants maintained higher biomass. Increasing drought stress led to decreased nitrogen, magnesium, and calcium concentrations in all treatments, with the reduction being less severe in inoculated plants. Catalase enzyme activity and carbohydrate increased with rising drought stress, with the increase being more pronounced in inoculated plants compared to non-inoculated ones. By promoting plant growth, nutrient uptake, and stress tolerance, P. indica inoculation has a significant potential to enhance crop productivity in extreme climate conditions.

Abstract Soil microbiota underpin ecosystem functionality yet are rarely targeted during ecosystem restoration. Soil microbiota recovery following native plant revegetation can take years to decades, while the effectiveness of soil inoculation treatments on microbiomes remains poorly explored. Therefore, innovative restoration treatments that target soil microbiota represent an opportunity to accelerate restoration outcomes. Here, we introduce the concept of ecological phage therapy—the application of phage for the targeted reduction of the most abundant and dominant bacterial taxa present in degraded ecosystems. We propose that naturally occurring bacteriophages—viruses that infect bacteria—could help rapidly shift soil microbiota towards target communities. Bacteriophages sculpt the microbiome by lysis of specific bacteria, and if followed by the addition of reference soil microbiota, such treatments could facilitate rapid reshaping of soil microbiota. Here, we experimentally tested this concept in a pilot study. We collected five replicate pre‐treatment degraded soil samples, then three replicate soil samples 48 hours after phage, bacteria, and control treatments. Bacterial 16S rDNA sequencing showed that phage‐treated soils had reduced bacterial diversity; however, when we combined ecological phage therapy with reference soil inoculation, we did not see a shift in soil bacterial community composition from degraded soil towards a reference‐like community. Our pilot study provides early evidence that ecological phage therapy could help accelerate the reshaping of soil microbiota with the ultimate aim of reducing timeframes for ecosystem recovery. We recommend the next steps for ecological phage therapy be (a) developing appropriate risk assessment and management frameworks, and (b) focussing research effort on its practical application to maximise its accessibility to restoration practitioners.

Abstract The objective of this work was to evaluate the response of citrus seedlings formed from buds challenged with Phytophthora citrophthora to the infection caused by this pathogen. For this, ‘Pera’ orange and ‘Tahiti’ acid lime scions were grafted on ‘Rangpur’ lime rootstock. The experiment was carried out in a greenhouse, in a completely randomized design in 2×4 factorial arrangement. Seedlings were challenged by a P. citrophthora isolate. Lesion size, plant height, and stem diameter were evaluated, as well as the following physiological attributes: internal CO2 concentration, transpiration, stomatal conductance, photosynthetic rate, and water use efficiency. In the ‘Pera’/‘Rangpur’ combination, seedlings formed by buds challenged by the pathogen exhibited smaller lesion sizes and higher photosynthetic rates. However, in the ‘Tahiti’/‘Rangpur’ combination, seedlings presented larger lesions, greater stem diameters, lower transpiration rates, and an increased water use efficiency. The challenge with the pathogen and the use of the ‘Pera’ orange/‘Rangpur’ lime combination (less sensitive to the disease) shows additive effects in the induction of resistance to gummosis.

In response to the escalating costs and diminishing efficiency of nitrogen fertilizers, the agricultural community is actively seeking sustainable alternatives that leverage natural nitrogen sources derived from biological N-fixation processes to enhance crop yield. This study investigates the combined effects of Klebsiella quasipneumoniae inoculation and varying nitrogen fertilizer doses on soil fertility, nutrient availability, and the yield and quality parameters of baby corn (Zea mays. L). The study involved the application of five nitrogen levels (0, 75, 150, 225, and 300 kg N ha-1) in conjunction with Klebsiella quasipneumoniae inoculum on HM-4 variety of baby corn, employing a comprehensive experimental design with five treatments and four replications. All treatments demonstrated increased ear count and weights of ear, silk, husk, edible cob, and biomass compared to the control. The study highlights the potential of Klebsiella quasipneumoniae inoculation in synergy with reduced nitrogen fertilizer to enhance total N contents in soil and positively impact baby corn yield and cob quality parameters. Optimal results were achieved with a 50% reduction in nitrogen fertilizer (150 kg N ha-1), emphasizing the importance of integrated nutrient management. The findings contribute valuable insights to sustainable agriculture, offering a promising strategy for increased baby corn production, improved nutritional quality, and environmental conservation. This integrated approach, involving microbial inoculation and nitrogen management, emerges as a key element in modern agricultural practices, promoting both productivity and nutritional content in baby corn crops.

Rice as a staple food for very large population suffers from various biotic and abiotic stresses. Among the biotic stresses, Blast, Bacterial leaf blight and Brown plant hopper (BPH) are considered to most potential threats that significantly affect the rice productivity. The use of chemical usage for controlling these diseases and pest attacks is not environmentally friendly and is expensive. Using Molecular Marker assisted backcross breeding program we have improved one of our hybrid parental line with 5 genes (xa13, Xa21, Pi54, Bph20 and Bph21). We have developed 15 near isogenic lines having similar agronomical characters as of recipient parent, they are promising for their direct induction in breeding program. Our results indicated that out of fifteen lines, Six lines i.e. GK 101-12, GK 101-15, GK 101-9, GK 101-5 and GK 101-2 out-performed in all the three locations tested, they had not only have very high level of resistance to BLB, Blast and BPH but also showed significantly higher yield compared to susceptible check as well as recipient parent. These lines may be used for improvement of our existing hybrid and also for developing of new hybrids with other CMS lines. These lines are also useful for developing new set of restorer lines through (R x R) and also useful for introgression of wide spectrum of resistance to the varieties/hybrid.

The study investigated the impact of the interaction between soybean and rhizobia on the assimilation apparatus functioning and biomass accumulation in different soybean lines with varying photoperiod sensitivity. Nearly isogenic lines (NILs) of soybean were used, with genes E1, E2, and E3 in different allelic states: Clark (e1E2E3), L80-5879 (E1e2e3), L63-3117 (e1e2E3), and L71-920 (e1e2e3). The experimental group for each line was treated with Bradyrhizobium japonicum 634b. Plants were grown under natural long-day conditions (16 hours). Growth indicators of the studied lines, such as relative growth rate (RGR), net assimilation rate (NAR), leaf area ratio (LAR), and specific leaf area (SLA), were analyzed, as well as the content of chlorophylls A and B in the V3 and V5 developmental stages. The results demonstrate that the influence of rhizobia on the functioning of the assimilation apparatus and biomass accumulation depends on the soybean line genotype. In the study, RGR, which characterizes the biomass accumulation rate, has similar trends to those observed with NAR, characterizing the assimilation apparatus's functioning. However, each line showed its own tendencies. For instance, in the short-day variety Clark, under bacterial influence, the value of RGR and NAR decreased. Additionally, LAR and SLA values indicated a reduction in the total photosynthetic surface area and leaf dry matter. Bacterial inoculation did not significantly affect the content of photosynthetic pigments in Clark leaves. Another short-day line, L80-5879, showed no significant impact of bacterial inoculation on biomass accumulation. However, soybean interaction with Bradyrhizobium japonicum 634b led to a decrease in leaf surface area and dry matter content. Probably, bacterial inoculation supported assimilation processes by increasing auxiliary chlorophyll b in photosystem I. A general trend of significant RGR reduction in neutral-day soybean lines, L63-3117 and L71-920, was identified under bacterial influence. The interaction with rhizobia differently affected LAR and SLA values, indicating distinct adaptive mechanisms to the interactions. In conditions of almost zero plant biomass accumulation, Bradyrhizobium japonicum 634b caused a decrease in the total photosynthetic surface area and chlorophyll a and b content in the L63-3117 line. In L71-920, bacterial inoculation had no effect on the total photosynthetic surface area, while leaf dry matter and photosynthetic pigment content decreased. The obtained results demonstrate that interaction with rhizobia can influence the functioning of the assimilation apparatus in soybeans with varying photoperiod sensitivity that is determined by genotype. It is important in improving soybean productivity and its application in agricultural practices.