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3 results on '"IGD motifs"'

1. Unleashing Cancer Cells on Surfaces Exposing Motogenic IGDQ Peptides

Author

Riccardo Marega, Federica De Leo, Valentina Corvaglia, Davide Bonifazi, Carine Michiels, Corvaglia, Valentina, Marega, Riccardo, De Leo, Federica, Michiels, Carine, and Bonifazi, Davide

Subjects
0301 basic medicine, Cell signaling, Cancer cells, cell migration, Surface Properties, 010402 general chemistry, 01 natural sciences, chemical gradient, Conserved sequence, Focal adhesion, Biomaterials, 03 medical and health sciences, Cell Movement, Cell Line, Tumor, Neoplasms, Humans, General Materials Science, Cell migration, Engineering (miscellaneous), cancer cell, cancer cells, chemical gradients, IGD motifs, peptide SAMs, Biotechnology, Microscopy, Confocal, biology, Water, General Chemistry, IGD motif, Biomaterial, Peptide SAMs, peptide SAM, 0104 chemical sciences, Cell biology, Fibronectin, 030104 developmental biology, Cell culture, Cancer cell, biology.protein, Phosphorylation, Chemical gradients, Gold, Peptides
Abstract

Thiolated peptides bearing the Ile-Gly-Asp (IGD) motif, a highly conserved sequence of fibronectin, are used for the preparation of anisotropic self-assembled monolayers (SAM gradients) to study the whole-population migratory behavior of metastatic breast cancer cells (MDA-MB-231 cells). Ile-Gly-Asp-Gln-(IGDQ)-exposing SAMs sustain the adhesion of MDA-MB-231 cells by triggering focal adhesion kinase phosphorylation, similarly to the analogous Gly-Arg-Gly-Asp-(GRGD)-terminating surfaces. However, the biological responses of different cell lines interfaced with the SAM gradients show that only those exposing the IGDQ sequence induce significant migration of MDA-MB-231 cells. In particular, the observed migratory behavior suggests the presence of cell subpopulations associated with a "stationary" or a "migratory" phenotype, the latter determining a considerable cell migration at the sub-cm length scale. These findings are of great importance as they suggest for the first time an active role of biological surfaces exposing the IGD motif in the multicomponent orchestration of cellular signaling involved in the metastatic progression.

Published
2016
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2. Cancer Cells: Unleashing Cancer Cells on Surfaces Exposing Motogenic IGDQ Peptides (Small 3/2016)

Author

Carine Michiels, Valentina Corvaglia, Davide Bonifazi, Riccardo Marega, Federica De Leo, Corvaglia, Valentina, Marega, Riccardo, De Leo, Federica, Michiels, Carine, and Bonifazi, Davide

Subjects
cell migration, Chemistry, Cell migration, General Chemistry, IGD motif, chemical gradient, Cell biology, Biomaterials, Cancer cell, IGD motifs, cancer cells, General Materials Science, peptide SAMs, chemical gradients, Biotechnology, cancer cell
Abstract

Thiolated peptides bearing the Ile-Gly-Asp (IGD) motif, a highly conserved sequence of fi bronectin, are used for the preparation of anisotropic self-assembled monolayers (SAM gradients) to study the whole-population migratory behavior of metastatic breast cancer cells (MDA-MB-231 cells). Ile-Gly-Asp-Gln-(IGDQ)-exposing SAMs sustain the adhesion of MDA-MB-231 cells by triggering focal adhesion kinase phosphorylation, similarly to the analogous Gly-Arg-Gly-Asp-(GRGD)-terminating surfaces. However, the biological responses of different cell lines interfaced with the SAM gradients show that only those exposing the IGDQ sequence induce signifi cant migration of MDA-MB-231 cells. In particular, the observed migratory behavior suggests the presence of cell subpopulations associated with a “stationary” or a “migratory” phenotype, the latter determining a considerable cell migration at the sub-cm length scale. These findings are of great importance as they suggest for the first time an active role of biological surfaces exposing the IGD motif in the multicomponent orchestration of cellular signaling involved in the metastatic progression.

Published
2016

3. Unleashing Cancer Cells on Surfaces Exposing Motogenic IGDQ Peptides.

Author

Corvaglia V, Marega R, De Leo F, Michiels C, and Bonifazi D

Subjects
Cell Line, Tumor, Cell Movement drug effects, Gold chemistry, Humans, Microscopy, Confocal, Peptides chemistry, Surface Properties, Water chemistry, Neoplasms pathology, Peptides pharmacology
Abstract

Thiolated peptides bearing the Ile-Gly-Asp (IGD) motif, a highly conserved sequence of fibronectin, are used for the preparation of anisotropic self-assembled monolayers (SAM gradients) to study the whole-population migratory behavior of metastatic breast cancer cells (MDA-MB-231 cells). Ile-Gly-Asp-Gln-(IGDQ)-exposing SAMs sustain the adhesion of MDA-MB-231 cells by triggering focal adhesion kinase phosphorylation, similarly to the analogous Gly-Arg-Gly-Asp-(GRGD)-terminating surfaces. However, the biological responses of different cell lines interfaced with the SAM gradients show that only those exposing the IGDQ sequence induce significant migration of MDA-MB-231 cells. In particular, the observed migratory behavior suggests the presence of cell subpopulations associated with a "stationary" or a "migratory" phenotype, the latter determining a considerable cell migration at the sub-cm length scale. These findings are of great importance as they suggest for the first time an active role of biological surfaces exposing the IGD motif in the multicomponent orchestration of cellular signaling involved in the metastatic progression., (© 2015 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.)

Published
2016
Full Text
View/download PDF

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