1. Polishing domestic wastewater on a subsurface flow constructed wetland
- Author
-
Paula M. L. Castro, A. Matos, I. Aguiar-Pinto Mina, Marta Costa, C. Sousa Coutinho Calheiros, Veritati - Repositório Institucional da Universidade Católica Portuguesa, and Universidade do Minho
- Subjects
Time Factors ,constructed wetland ,Colony Count, Microbial ,Sewage ,02 engineering and technology ,Plant Science ,010501 environmental sciences ,01 natural sciences ,Waste Disposal, Fluid ,domestic wastewater ,RNA, Ribosomal, 16S ,Cluster Analysis ,DGGE ,020701 environmental engineering ,education.field_of_study ,Ecology ,Pollution ,Biota ,6. Clean water ,Constructed wetland ,RNA, Bacterial ,Biodegradation, Environmental ,Wastewater ,Sewage treatment ,Water Microbiology ,Environmental Monitoring ,DNA, Bacterial ,Population ,0207 environmental engineering ,microbial communities ,Industrial Waste ,Microbial communities ,Poaceae ,Water Purification ,Domestic wastewater ,Environmental Chemistry ,Phragmites australis ,education ,Subsurface flow ,0105 earth and related environmental sciences ,Science & Technology ,Bacteria ,Portugal ,business.industry ,Denaturing Gradient Gel Electrophoresis ,Environmental engineering ,Coliform bacteria ,Fecal coliform ,13. Climate action ,Wetlands ,Environmental science ,business - Abstract
Microbial monitoring of constructed wetlands (CWs) treating domestic wastewater is generally scarce, despite the need of more knowledge about its biocenosis. The sanitation quality of a wastewater treated in a CW is a crucial aspect, mainly when the receiving water body is used as a swimming and/or recreation area. The present study was carried out in a horizontal subsurface flow CW planted with Phragmites australis receiving pre-treated domestic wastewater (mean flow 50 m(3) day(-1)), from a population of about 300 inhabitants. The monitoring programme undertaken during the first year operation, revealed removal efficiencies of 61% BOD5, 44% COD, and 65% TSS for inlet water with ca. 90 mg L-1 BOD5, 157 mg L-1 COD, and 17 mg L-1 TSS. Total Coliform (TC) and Faecal Coliform (FC) bacteria were removed from wastewater (mean inlet values of 5 x 10(6) CFU 100 mL(-1) TC and of 9 x 10(5)CFU 100 mL(-1) FC), with efficiencies of 92 and 97%, respectively. The dynamics of microbial communities established in the system assessed by polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE), had revealed a high bacterial diversity within the system, with no relevant differences in composition at the CW inlet and outlet but exhibiting temporal differences in bacterial communities., Isabel A-P. Mina wishes to thank Aguas do Ave S.A., for the permission to carry out this study on Parada do Bouro Treatment Plant, and for the financial support conceded.Cristina S.C. Calheiros wishes to thank a research grant from Fundacao para a Ciencia e Tecnologia (FCT), Portugal (SFRH/BPD/63204/2009).
- Published
- 2011