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Background: Motor Vehicle Accidents (MVAs) are major health hazards in Saudi Arabia and may result in multiple injuries. Moreover, it is one of the most common causes of morbidity and mortalities, worldwide. Thus, the awareness of the bystanders who are the first to witness the MVAs is important. Objective: The purpose of this study was to evaluate of the level of the knowledge among the adults in Al-Madinah in terms of dealing with multiple trauma victims at the scene of the accident. Methodology: A cross sectional study was conducted in Al-Madinah, Saudi Arabia during October and November 2017 on a university 621 persons participated in the study. A self-administered questionnaire was used. The average age of participants was ranged from 18 to -49 years with male: female ratio of 1.18:1. Results: Out of 621 participants, 107 (17.2%) exhibited a low level of knowledge, 311 (50.1%) have a good level, and 203 (32.7%) showed an excellent level. Gender was found to be statistically significant in favor of male participants (p=0.004). Participants with a high degree of education had been found to have a better knowledge in dealing with trauma patients at the scene of the accident (p=0.009). Participants who attended a training course showed a better knowledge in dealing in such situations (p=0.001). Conclusion: Promoting courses that target educating and training public on the appropriate way of dealing with trauma victims may be helpful.