1. Study of a suitable fish plasma for in vitro culture of glochidia Hyriopsis myersiana
- Author
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Uthaiwan, Kovitvadhi, Pakkong, Pannee, Noparatnaraporn, Napavarn, Vilarinho, Laura, and Machado, Jorge
- Subjects
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FISH genetics , *FISH farming - Abstract
Several organic and inorganic sources from the plasma of different fish species and horse serum were utilized as additives to the artificial culture M199 medium to improve glochidial survival and transformation of Hyriopsis myersiana. After 2–3 days of culturing in the medium containing plasma of Nile tilapia or hybrid catfish, striped catfish or horse serum, the glochidia presented significantly (P<0.05) lower percentage survival compared to medium containing common carp plasma. The highest (93.77±3.0) and lowest (32.42±5.85) percentage survival rates of glochidia were found with common carp and striped catfish plasma, respectively. After 10 days, relevant signs of glochidia transformation, such as the foot and mantle edge, were observed. In all assays, the glochidia transformation reached 100% most probably due to the exchange of the medium at the fifth day and the addition of 1 ml of distilled water at the ninth day of culturing. The intense mobility of juveniles in the medium containing the common carp plasma indicated excellent culture conditions. The ideal density for this plasma corresponded to 150–200 glochidia per culture dish.The present results suggest that M119 medium complemented with the common carp plasma and the medium exchange during culturing period may constitute a functional process to prepare an in vitro culture for freshwater mussels, particularly H. myersiana. The most relevant amino acids for a successful development are CIT, GLX, LEU, PRO, THR and ALA particularly with the contents in the common carp plasma. [Copyright &y& Elsevier]
- Published
- 2002
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