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3 results on '"Hyriopsis myersiana"'

1. Study of a suitable fish plasma for in vitro culture of glochidia Hyriopsis myersiana

Author

Uthaiwan, Kovitvadhi, Pakkong, Pannee, Noparatnaraporn, Napavarn, Vilarinho, Laura, and Machado, Jorge

Subjects
*FISH genetics, *FISH farming
Abstract

Several organic and inorganic sources from the plasma of different fish species and horse serum were utilized as additives to the artificial culture M199 medium to improve glochidial survival and transformation of Hyriopsis myersiana. After 2–3 days of culturing in the medium containing plasma of Nile tilapia or hybrid catfish, striped catfish or horse serum, the glochidia presented significantly (P<0.05) lower percentage survival compared to medium containing common carp plasma. The highest (93.77±3.0) and lowest (32.42±5.85) percentage survival rates of glochidia were found with common carp and striped catfish plasma, respectively. After 10 days, relevant signs of glochidia transformation, such as the foot and mantle edge, were observed. In all assays, the glochidia transformation reached 100% most probably due to the exchange of the medium at the fifth day and the addition of 1 ml of distilled water at the ninth day of culturing. The intense mobility of juveniles in the medium containing the common carp plasma indicated excellent culture conditions. The ideal density for this plasma corresponded to 150–200 glochidia per culture dish.The present results suggest that M119 medium complemented with the common carp plasma and the medium exchange during culturing period may constitute a functional process to prepare an in vitro culture for freshwater mussels, particularly H. myersiana. The most relevant amino acids for a successful development are CIT, GLX, LEU, PRO, THR and ALA particularly with the contents in the common carp plasma. [Copyright &y& Elsevier]

Published
2002
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2. Scanning electron microscopy of glochidia and juveniles of the freshwater mussel,Hyriopsis myersiana

Author

Jorge Machado, Napa Varn Noparatnaraporn, Kannika Chatchavalvanich, and Kovitvadhi Uthaiwan

Subjects
Scanning electron microscope, Periostracum, media_common.quotation_subject, Mussel, Anatomy, Biology, Hyriopsis myersiana, Brood, Ultrastructure, Juvenile, Animal Science and Zoology, Metamorphosis, Developmental Biology, media_common
Abstract

Summary The ultrastructure of early stages of the mussel, Hyriopsis (Limnoscapha) myersiana (Lea, 1856), was observed by scanning electron microscopy from the glochidial period until the onset of the juvenile stage 10 days later. Further observations were performed for an additional 13 days to assess juvenile development. Glochidia extracted from the brood chambers have a hookless, semi-oval and equivalve calcareous shell with numerous pores in the internal surface, pits in the external surface and cuticular spines in the ventral region. Keratin fibers with a random arrangement in the cuticle of the glochidial shell were also detected. The appearance of the foot within 10 days of in vitro glochidial culture was considered the main feature of metamorphosis to the juvenile stage. Another change during the following 13 days was the formation of a new periostracum exhibiting growth lines under the old glochidial shell. This development occurs mainly in the anterior region and is followed by hardening of the p...

Published
2001

3. Culture of glochidia of the freshwater pearl mussel Hyriopsis myersiana (Lea, 1856) in artificial media

Author

Kovitvadhi Uthaiwan, Jorge Machado, and Napavarn Noparatnaraporn

Subjects
biology, Ecology, media_common.quotation_subject, Aquatic Science, Hyriopsis myersiana, biology.organism_classification, Bivalvia, Oreochromis, Animal science, Freshwater pearl mussel, Artificial culture, Juvenile, Metamorphosis, Mollusca, media_common
Abstract

The freshwater pearl mussel, Hyriopsis myersiana (Limnoscapha) (Lea, 1856) was cultured in two artificial media at 23±2°C. Each artificial medium contained a mixture of M199, (Life Technologies, No. 71N0262) horse serum or fish ( Oreochromis niloticus ) artificial medium plasma as a protein source, and antibiotics/antimycotics at a ratio of 2:1:0.5. Glochidia were reared until they became juveniles, i.e. until the mantle and foot could be observed under a light microscope. The duration of glochidia development until the juvenile stage was 9–10 days in both media. After 1 month of controlled feeding with phytoplankton, the juveniles showed an elongate of shell with several growth lines. The more suitable artificial culture formula for the transformation from glochidia to juvenile stage was the medium containing protein from fish plasma. Survival from glochidia to juvenile stage was up to 85.3±3.9% in fish plasma, while it was equal to 46.2±12.7% in horse serum. The transformation from glochidia to juvenile stage was up to 84.3±2.3% in fish plasma, while it was equal to 44.3±8.9% in horse serum. Percentage survival and transformation from glochidia to juvenile stage were significantly higher in fish plasma than in horse serum ( P

Published
2001

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