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Made available in DSpace on 2019-10-05T06:21:11Z (GMT). No. of bitstreams: 0 Previous issue date: 2018-12-01 Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Eighteen semen samples from nine Santa Ines rams were cryopreserved in order to study the cryoprotective capacity of dimethylacetamide (DMA) at two concentrations (3% and 6%), and its interaction with trehalose (TRE, 100 mOsmol). A further objective of this study was to evaluate in vivo fertility of ram semen cryopreserved with dimethylacetamide. The control treatment was an extender medium with 6% glycerol (GLY). Five treatments were examined: 6% GLY, 3% DMA, 6% DMA, 3% DMA + TRE, and 6% DMA + TRE. After thawing, the kinetic sperm parameters were analyzed using a computerized system. Sperm viability was observed using a multiple parameter sperm staining with propidium iodide (for plasmatic membrane integrity), JC-1 (for mitochondrial membrane potential), and FITC-PSA (for acrosomal integrity). The isosmotic extender with GLY was the most effective medium for the maintenance of sperm characteristics, compared to extenders containing DMA as cryoprotectant. Regarding fertility, the extender medium with 3% DMA may be a safe alternative for sperm cryopreservation, and does not compromise the fertility rates. The addition of TRE decreased the kinetic sperm parameters; however, a positive effect on the integrity of the plasmatic and acrosomal membranes was observed. Univ Fed Bahia, Sch Vet & Anim Sci Med, Ave Adhemar de Barros 500, BR-40170110 Salvador, BA, Brazil Sao Paulo State Univ, Coll Vet Med & Anim Sci, BR-18618000 Botucatu, SP, Brazil Embrapa Coastal Tablelands, Anim Reprod Biotechnol Lab, Ave Beira Mar 3250, BR-49025040 Aracaju, Sergipe, Brazil Sao Paulo State Univ, Coll Vet Med & Anim Sci, BR-18618000 Botucatu, SP, Brazil

Made available in DSpace on 2016-04-01T18:44:20Z (GMT). No. of bitstreams: 0 Previous issue date: 2014. Added 1 bitstream(s) on 2016-04-01T18:49:50Z : No. of bitstreams: 1 ISSN1517-784X-2014-19-02-69-77.pdf: 222564 bytes, checksum: 36c0abdb3ae1af4a7b499f28bad72e61 (MD5) Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Vinte amostras de semen de dez carneiros da raça Santa Inês foram criopreservadas com oobjetivo de verificar o efeito da adição de trealose (100mOsmol) (grupo 1 –TRIS+TRE) ao diluidor Tris-gema de ovo-glicerol (grupo 2 –TRIS), associado ou não ao quelante de cálcio EDTA (grupo 3 –TRIS+TRE+EDTA), sobre a viabilidade espermática pós-descongelação. Após avaliação, as amostras diluídas foram resfriadas à 5oC e congeladas em vapor de nitrogênio líquido. Após descongelação, os parâmetros de cinética espermática foram analizados por um sistema computadorizado (CASA). A viabilidade espermática foi observada através da coloração para avaliação multi-paramétrica com iodeto de propídio (MPI, membrana plasmática íntegra), JC-1 (PMM, potencial de membrana mitocondrial) e FITC-PSA (IAC, integridade acrossomal). A análise estatística foi realizada através do programa estatístico SAS, aplicando o procedimento MEANS para a análise descritiva e o procedimento GLM com o teste de Student-Newman-Keuls (SNK) para a comparação dos parâmentros espermáticos entre os diferentes grupos (com P0,05) entre os grupos. Pode-se concluir que a adição da trealose, associada ou não ao EDTA, proporcionou efeito negativo para criopreservação do sêmen ovino. Twenty semen samples from ten rams of the Santa Inês breed were cryopreserved with the goal of verifying the effect of trehalose addition (100mOsmol) (group 1 -TRIS+TRE) to a Tris-egg yolk-glycerol extender (group 2 -TRIS), associated or not to the calcium chelator EDTA (group 3 -TRIS+TRE+EDTA), on the post-thaw sperm viability. After evaluation, the diluted semen samples were cooled at 5C and frozen in liquid nitrogen steam. After thawing, the kinetic sperm parameters were analyzed by a computer analyses system (CASA). The sperm viability was observed by using the multiple sperm parameter staining with propidium iodide (PMI, plasmatic membrane integrity), JC-1 (MMP, mitochondrialmembrane potential) and FITC-PSA (ACI, acrosomal integrity). The statistical analysis was performed using the SAS software, applying the MEANS procedure for descriptive analysis and the GLM procedure with the Student-Newman-Keuls test (SNK) for comparisonof sperm parameters among different groups(with P0.05) among the groups. It can be concluded that the trehalose addition to the TRIS extender, associated or not to EDTA, had detrimental effect on cryosurvivalram sperm. Universidade Federal da Bahia (UFBA) Universidade Estadual Paulista Júlio de Mesquita Filho, Departamento de Reprodução Animal e Radiologia Veterinária, Faculdade de Medicina Veterinária e Zootecnia de Botucatu, Botucatu, Distrito de Rubião Júnior, s/n, Rubião Júnior, CEP 18610250, SP, Brasil Universidade Estadual Paulista Júlio de Mesquita Filho, Departamento de Reprodução Animal e Radiologia Veterinária, Faculdade de Medicina Veterinária e Zootecnia de Botucatu, Botucatu, Distrito de Rubião Júnior, s/n, Rubião Júnior, CEP 18610250, SP, Brasil