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Purpose: The purpose of this study was to define and clarify the concept of the family caregivers’ perceived value of caring for older patients. Methods: The hybrid model of Schwartz-Barcott and Kim was used to perform the concept analysis of perceived value of caring. Concept analysis was conducted in three phases: the theoretical phase, fieldwork phase, and final analysis phase. Results: The perceived value of caring was found to have four dimensions and nine attributes. The providing care dimension consisted of two attributes—care behavior and care attitude—and the goals and expectation dimension included three attributes—social norms, family norms, and personal norms. The other two dimensions consisted of two attributes each: a belief in usefulness and a belief in meaning in the building beliefs dimension and life expansion and insight into the future in the suffering sublimation dimension. Finally, perceived value of caring can be defined as the personal belief that behavior or attitude of providing physical, mental, and social services to those in need of care is usefulness and important based on one’s goals and criteria, and with the personal effort of sublimating suffering that arises from care. Conclusion: Based on the study findings, it is suggested that the use of the perceived value of caring should expand in nursing phenomena.

A strategy for effectively improving oocyte quality and cryotolerance during vitrification via nanoparticle-mediated melatonin delivery is reported.

Purpose: The purpose of this study was to describe and understand the meaning and essence of care experienced by long-term-care-hospital nurses during the COVID-19 pandemic. Methods: This study is a phenomenological study by Colaizzi (1978) to understand and identify the meaning and essence of long-term-care-hospital nurses' experiences after the COVID-19 outbreak. Data collection was conducted from April to June 2021 for 10 nurses working at a long-term-care hospital. Interviews were conducted face-to-face or non-face-to-face. The face-to-face interviews were conducted in a space following COVID-19-mandated quarantine rules. Results: Seven theme clusters and 24 themes emerged: chaos experienced during the early stage of the pandemic, lack of a standardized care protocol hindering care delivery in long-term-care hospitals, sympathizing with the sorrows of patients and their families, taking the burdensome task of COVID-19 infection control as a nurse, COVID-19 vaccine-related anxiety, anger at the reality of long-term-care hospitals and the stigmatizing society, and hope for recovery from the disaster caused by COVID-19. Conclusion: This study will be helpful in establishing long-term-care policies and systems that will help improve the ability of long-term-care hospitals to cope with a pandemic in the post-COVID-19 era.

Purpose: This study aimed to identify the effect of visiting care service users’ functional status and caregiving appraisal on the somatization of primary caregivers.Methods: This study was a descriptive correlation study. The study subjects included 152 caregivers who care for visiting care service users at three long-term centers located in Metropolitan City B. Data collection was conducted with the structured self-report and observer-report questionnaires from July 15th to September 15th, 2020. The collected data were analyzed with descriptive statistics, t-test, one-way ANOVA, Pearson’s correlation coefficient, and hierarchical multiple regression using the IBM SPSS/WINdow 26.0 program.Results: As a result of conducting hierarchical regression analysis in three stages to identify the influencing factors on somatization of primary caregivers, satisfaction(β=-.26, p=.001) contributed the most to explaining the somatization, followed by subjective health condition(β=.24, p=.008) and burden(β=.22, p=.008).Conclusions: Increasing subjective health status, low burden, and high satisfaction experience with the primary caregiver will ultimately improve their ability to enjoy a healthy life.

Background Nurses have an essential role and responsibility to work at the forefront of patient care during the COVID-19 pandemic. Although the press and public have praised the dedication of nurses in the COVID-19 pandemic, there are several points to consider for nursing professional development. The purpose of this study is to collect the experiences of the nursing profession in the COVID-19 pandemic through interviews, seek improvements for the development of the nursing profession, and suggest directions for the future. Method This qualitative study adopts semi-structured interviews analyzing the nursing experience of the COVID-19 pandemic based on the professional practice models (PPMs). Ten nurses with at least two years of working experience and thorough work changes in the COVID-19 pandemic from various settings have participated in the study Results We identified thirty-nine problematic codes and nineteen improvement codes which mapped to 12 key concepts and corresponded to 6 constructs of the PPM model. Conclusions Nurses had to take on tasks beyond their duties in urgent situations, which restrained nurses from concentrating on their work. Clarifying working boundaries is fundamental for collaborative care and independent nursing practice. Collaboration and communication among healthcare workers based on mutual understanding can create a respectful working environment. Although there were many difficulties due to the uncertain situation, we can find that the nursing profession can make achievements through systematic and organizational support for sticking to the basics of nursing, securing technical expertise, cultivating critical thinking, and developing various professional attributes. In this way, the establishment of roles based on professional values and duties and the ascertainment of clear boundaries for nursing will ultimately help to improve the quality of patient care.

The efficiency of gene therapy is often dictated by the gene delivery system. Cationic polymers are essential elements of gene delivery systems. The relatively cheap cationic polymer, polyethyleneimine, has high gene delivery efficiency and is often used for gene delivery. However, the efficiency of gene therapy with polyethyleneimine-pDNA polyplex (PEI) is low. Human mesenchymal stem cells transfected with polyethyleneimine and a plasmid carrying the important osteogenic differentiation gene runt-related transcription factor 2 (RUNX2) accumulated DNA double-strand breaks and mitochondrial damage proportional to the amount of polyethyleneimine, reducing viability. Genomic/cellular stabilizer mediating RUNX2 delivery (GuaRD), a new reagent incorporating RS-1 NPs developed in this study, promoted DNA repair and prevented the accumulation of cell damage, allowing the delivery of pRUNX2 into hMSCs. while maintaining genome and mitochondrial stability. DNA damage was significantly lower and the expression of DNA repair-related genes significantly higher with GuaRD than with PEI. In addition, GuaRD improved mitochondrial stability, decreased the level of reactive oxygen species, and increased mitochondrial membrane potential. Osteogenic extracellular matrix (ECM) expression and calcification were higher with GuaRD than with PEI, suggesting improved osteogenic differentiation. These results indicate that lowering the cytotoxicity of PEI and improving cell stability are key to overcoming the limitations of conventional gene therapy, and that GuaRD can help resolve these limitations.

Background: Fear may be critical in explaining individual and social behaviors. This study investigates the association between COVID-19 fear and health behavior and subjective health status changes of call center workers in the COVID-19 era. Methods: This cross-sectional study uses an online survey with 339 call center workers. We measured COVID-19 fear, health behaviors, and subjective health, and analyzed with the Macnemar or paired t-test, ANOVA or χ2 test, Scheffe’s test, and multiple linear regression. Results: COVID-19 fear was associated with poor stress management, shorter sleep hours, and binge eating. Moreover, COVID-19 fear and time pressure at work were negatively associated with subjective health status. Conclusion: Strengthening the support system for call center workers to manage the COVID-19 fear might be essential. Moreover, there is a need to improve dense environments and reduce time pressure by ensuring adequate rest time and increasing physical activity.

Infectious bronchitis virus (IBV) is a major pathogen in poultry. The genotypes of IBV vary considerably, and their antigenicity may differ. Nationwide surveillance in South Korea was performed to determine the prevalence and distribution of IBV and its genotypes. By both active and passive surveillance, a total of 939 samples were collected and tested for IBV detection by pathogen-specific reverse transcriptase-PCR. IBV RNA-positive samples were inoculated in embryonated eggs for virus isolation. IBV was genotyped and analyzed phylogenetically based on a partial nucleotide sequence of the S1 gene. A total of 114 IBV strains were isolated; 34 (30.9%) of the 110 samples obtained by passive surveillance, and 80 (9.7%) of the 829 samples obtained by active surveillance, were positive. Most IBVs in both groups were isolated from broilers. Five genotypes (QX-like, B4-like, KM91-like, K40/09-like, and 20AD17-like) were observed in South Korea, with the QX-like genotype being the most common, and the 20AD17-like genotype being a novel genotype. These findings will help to maximize protection against IBV infection by providing a reference for the selection of an avian vaccine for IBV in South Korea.Vigilancia nacional del virus de la bronquitis infecciosa en Corea del Sur del año 2020 al 2021. El virus de la bronquitis infecciosa (IBV) es un patógeno importante en la avicultura. Los genotipos del virus de la bronquitis varían considerablemente y su antigenicidad puede ser diversa. Se realizó un estudio de vigilancia a nivel nacional en Corea del Sur para determinar la prevalencia y distribución del virus de bronquitis y sus genotipos. Mediante vigilancia activa como pasiva, se recolectaron un total de 939 muestras y se analizaron para la detección del virus de la bronquitis infecciosa mediante transcripción reversa y PCR específica para este patógeno. Se inocularon muestras positivas para ARN del virus de bronquitis en huevos embrionados para el aislamiento del virus. Los virus de bronquitis se genotipificaron y analizaron filogenéticamente basándose en una secuencia parcial de nucleótidos del gene S1. Se aislaron un total de 114 cepas del virus de bronquitis; 34 (30.9%) de las 110 muestras obtenidas por vigilancia pasiva y 80 (9.7%) de las 829 muestras obtenidas por vigilancia activa resultaron positivas. La mayoría de los virus de bronquitis en ambos grupos se aislaron de pollos de engorde. Se observaron cinco genotipos (similares a QX, similares a B4, similares a KM91, similares a K40/09 y similares a 20AD17) en Corea del Sur, siendo el genotipo similar a QX el más común y el genotipo similar a 20AD17 que ha sido un genotipo de nueva aparición. Estos hallazgos ayudarán a maximizar la protección contra la infección por el virus de la bronquitis infecciosa al proporcionar una referencia para la selección de vacunas aviares para bronquitis infecciosa en Corea del Sur.

Purpose: The purpose of this study was to identify the relationship between intrinsic motivation and learning outcomes of nursing college students who took the Fundamentals of nursing as a problem-based learning method.Methods: In this cross-sectional study, we identified the intrinsic motivations of 114 nursing students who completed problem-based learning using the Intrinsic Motivation Inventory. The t-test was conducted to identify differences according to intrinsic motivation, and correlation analysis was performed to confirm the relationship between intrinsic motivation and learning outcomes.Results: The group with higher intrinsic motivation showed higher scores in all domains of self-assessed learning outcomes than the lower group. It was the ‘Relatedness with an instructor’ that showed the highest correlation with the learning outcomes in the domains of intrinsic motivation.Conclusion: Problem-based learning is an effective learning method for cultivating the competencies needed for nurses. The intrinsic motivation of students is an important factor in the performance of problem-based learning. For the efficiency of problem-based learning, efforts should be made to develop and apply autonomy-supportive interventions that can enhance intrinsic motivation.

Background and aims Runting-stunting syndrome (RSS) in chickens, also known as malabsorption syndrome, which is characterized by mild to severe enteritis and diagnosed through typical histopathologic examination as well as clinical signs, results in considerable economic losses. Despite the many studies carried out over decades to determine the etiologic agents of RSS involved in the disease, several outbreaks remained without the elucidation of, potentially multiple, etiologies involved. Methods We performed comparative analysis of viral metagenomes from four chicken flocks affected with RSS using next-generation sequencing. Primers for the detection of chicken enteric viruses were designed from the sequencing data obtained with metagenomics. Multiplex reverse transcription–polymerase chain reaction (PCR) and PCR were performed to detect a variety of etiological agents previously described in natural cases of RSS. Results The most abundant viral families identified in this study were Astroviridae, Picornaviridae, Parvoviridae, Caliciviridae, Reoviridae and Picobirnaviridae. Chicken astrovirus sequences were present in all four samples, suggesting an association between chicken astrovirus and RSS and chicken astrovirus as a candidate pathogen responsible for RSS. Picobirnavirus and the newly identified chapparvovirus were found in chickens in the Republic of Korea for the first time, and the genetic diversity of enteric viruses and viral communities was showed. Conclusions Chicken astrovirus was consistently detected in broilers affected with RSS and the result of this study may contribute to knowledge of enteric diseases and viruses in chickens.

This study intends to provide the grounds for improving clinical practice curriculum reflecting the reality and demands of the clinical practice of nursing by identifying the needs of nursing students for clinical practice education and the experiences of clinical practice education perceived by graduates. A convergent parallel mixed-methods design was applied. According to the results of the quantitative study, application of core basic nursing skills based on expertise and skills and cooperation and communication were considered as important, and achievement was also high. According to the results of the qualitative study, it is essential to use such teaching methods like simulation so that students can improve their skill competencies. Additionally, quality improvement of clinical practice instructors was demanded to enhance the quality of clinical practice education. This study is significant because it established the future direction for the development of a clinical practice curriculum to enhance professional competencies for nurses in the future based on the needs of nursing students and graduates. It is necessary to develop an integrated assessment tool to measure achievement as well as develop a clinical practice curriculum.

Background In July 2015, the carcasses of 11 cockatiels were submitted for disease diagnosis to the Avian Disease Division of the Animal and Plant Quarantine Agency of Korea. The cockatiels, which appeared dehydrated and underweight, had exhibited severe diarrhea and 22 % mortality over 2 weeks. Traditional diagnosis did not reveal the causes of these symptoms. Methods We conducted metagenomics analysis on intestines and livers from the dead cockatiels using Illumina high-throughput sequencing. To obtain more accurate and longer contigs, which are required for further genetic characterization, we compared the results of three de novo assembly tools (metaSPAdes, MEGAHIT, and IDBA-UD). Results Sequence reads of Campylobacter jejuni (C. jejuni) and Chlamydia psittaci (C. psittaci) were present in most of the cockatiel samples. Either of these bacteria could cause the reported symptoms in psittaciformes. metaSPAdes (ver.3.14.1) identified the 1152 bp flaA gene of C. jejuni and the 1096 bp ompA gene of C. psittaci. Genetic analysis revealed that flaA of C. jejuni was recombinant between C. jejuni and Campylobacter coli, and that ompA of C. psittaci isolated from cockatiel was closely related to strains isolated from humans. Conclusions C. jejuni and C. psittaci were detected in cockatiels in the Republic of Korea using metagenomic analysis. This approach is useful for understanding pathogens of pet birds. Three de novo assemblers were compared to obtain accurate contigs from large quantities of reads, and sequences of C. jejuni and C. psittaci generated by metaSPAdes were analyzed.

Work-to-family conflicts (WFC), an aggravating factor of stress, may affect cardiovascular health. However, the link between WFC and cardiovascular health behaviors is not fully defined. This study intends to identify the mediating effect of coping strategy on the relation between WFC with cardiovascular health behaviors, such as smoking, stress management, increased physical activity, and healthy diet habits. The study is a cross-sectional online survey with 358 call center counselors. We adopted a linear regression to confirm the relationship between WFC, coping strategy, and cardiovascular health behavior, which are the main variables of the study. Maladaptive coping partially mediated the relationship between WFC and cardiovascular health behavior. Based on the results, we insist that a support system to reduce WFC is necessary, as well as increasing the availability of resources and policies to reduce WFC in the workplace and find ways to improve maladaptive coping to promote healthy behaviors of call center workers.

Physical attraction, perceived in terms of satisfaction with body image and interpersonal relationships, can be an essential factor in an individual’s emotional and social well-being. However, empirical studies that link physical attraction to health are limited. This study explores the mediating effect of life satisfaction on the relation between perceived physical attractiveness and lifestyle of health, making an effort to reach an optimal state in physical, emotional, social, spiritual, and intellectual domains in Korean adults. Four hundred fifty-nine adults in a community setting participated in this cross-sectional study. The results showed positive associations among physical attractiveness, life satisfaction, and health-promoting lifestyle after adjusting for age, gender, marital status, education, and job status. Life satisfaction partially mediated the relationship between perceived physical attractiveness and a higher lifestyle of health (z = 2.80, p = 0.005). For health promotion and disease prevention, positive psychology can be a suitable strategy. Physical attractiveness and life satisfaction can be important keys to maintain health-promoting lifestyle behavior.

Effective patient-provider communication is critical to promote patient satisfaction, encourage patient involvement in care, and improve health outcomes. Although prior HCI works aim to enhance the dyadic communication by improving patients’ communication skills, little is known about healthcare providers’ communication work to facilitate effective communication with their child patients. Through semi-structured interviews with 10 healthcare providers and clinic observations, our study identified four strategies that providers used in their communication with patients: building rapport, developing familiarity with care settings, respecting patients’ communication modes and preferences, and delegating small decision-making and directing questions to patients. Based on these strategies, we discuss three key elements that providers value and work toward to achieve effective communication in pediatric care practice. Our study also uncovers the detailed process of how the providers develop their strategies to tailor their communication to the patients’ specific needs and preferences, and we describe design opportunities for communication technology.

Infectious bursal disease (IBD) is one of the most important immunosuppressive diseases of young chickens, causing considerable economic losses to the poultry industry. More than 30 years ago, an antigenic variant (av) pathotype of the IBD virus (IBDV) was reported to originate in, and subsequently spread among, poultry farms in the USA. Recently, a novel avIBDV lineage was identified in China and was shown to exhibit clear differences in its pathogenicity as well as molecular characteristics compared with the previously isolated variant strains. In this study, we conducted a passive surveillance of chicken carcasses submitted to our research division from June-December 2019, and detected the IBDV strains by reverse transcription PCR. Five avIBDV strains were isolated, and their pathogenicity was determined by necropsy and molecular analysis. Additionally, a coinfection field case involving an avIBDV strain and a very virulent IBDV (vvIBDV) strain was identified. Multiple sequence alignment and phylogenetic analysis of partial viral protein 1 (VP1) and hypervariable region (hv) VP2 genes revealed that those strains originated from two different avIBDV lineages. The co-occurrence of two sub-groups of avIBDVs in South Korea confirms for the first time the evolution of antigenic variant IBDV strains, and highlights the urgency for the development of new strategies for IBDV intervention in South Korea.RESEARCH HIGHLIGHTS Five avIBDV strains were identified in South Korea by passive surveillance test in 2019.A coinfection between two IBDV strains from different genogroups was reported in a field case.By phylogenetic analysis, Korean avIBDVs belonged to two distinct lineages of antigenic variant genogroup.

Modifiable lifestyle behaviors, such as lack of physical activity, smoking, and unhealthy diet, are associated with the risk of cardiovascular diseases in postmenopausal women, in addition to other risk factors, such as aging and physiological changes. Therefore, it is necessary to emphasize the importance of encouraging healthy lifestyles and health-promoting behaviors among postmenopausal women, to achieve a better health status. However, it is difficult to modify lifestyle and maintain that change. This study was aimed at identifying the factors that affect the maintenance of healthy lifestyle habits in postmenopausal women, using various theoretical models. This qualitative study included focus-group interviews with 21 Korean postmenopausal women aged 54 to 69 years. A theory-guided thematic analysis was performed based on the Health Belief Model, Self-Determination Theory, Social Cognitive Theory, and Theory of Planned Behavior. As a result, facilitators and inhibitors to healthy lifestyle modification and maintenance are identified. Various sources of motivation and reinforcement are important for menopausal women to maintain a healthy lifestyle. Autonomy support and self-regulation strategies play an important role in integrating health-promoting behaviors into a daily routine. In addition to personal effort, a social support system is also important to help individuals maintain a healthy lifestyle

Objective This study examined the associations of work-family conflicts (WFC) with problem drinking and the mediating effect of depression among Korean married workers. Methods The data of 2386 married workers were used from the 2017 Korean Welfare Panel Study. The data classified into two groups, young adults and middle-aged based on age 45. The association of WFC with problem drinking and the mediating effect of depression was assessed by combining logistic and linear regression. Results WFC was associated with problem drinking, and the mediating effect of depression was significant in the middle-aged group. Meanwhile, depression only has a direct effect on problem drinking in the young adult group. Conclusion A strategy for treating depression may be important for countering problem drinking. However, an age-tailored intervention should be considered WFC supporting middle-aged workers.

We performed viral metagenomics analysis of Japanese quail affected with enteritis to elucidate the viral etiology. Metagenomics generated 21,066,442 sequence reads via high-throughput sequencing, with a mean length of 136 nt. Enrichment in viral sequences suggested that at least three viruses were present in quail samples. Coronavirus and picornavirus were identified and are known as pathogens causing quail enteritis that match the observed morphology. Abundant reads of coronavirus from quail samples yielded four fragment sequences exhibiting six genomes of avian coronavirus. Sequence analysis showed that this quail coronavirus was related to turkey coronavirus and chicken infectious bronchitis virus. Quail picornavirus 8177 bp in size was identified and was similar to the QPV1/HUN/01 virus detected in quails without clinical symptoms in Hungary with 84.6% nucleotide and 94.6% amino acid identity. Our results are useful for understanding the genetic diversity of quail viruses. Further studies must be performed to determine whether quail coronavirus and quail picornavirus are pathogens of the digestive tract of quails.

Infectious laryngotracheitis (ILT), fowlpox (FP), and reticuloendotheliosis are important poultry diseases caused by gallid herpesvirus 1 (ILTV), fowlpox virus (FWPV), and reticuloendotheliosis virus (REV), respectively. Coinfections with ILTV and FWPV occur naturally in chickens, and FP in its more virulent wet form is characterized by diphtheritic lesions and easily confused with ILT. Moreover, the insertion of only partial REV-LTR or a nearly full-length REV into the FWPV genome, located between the ORF 201 and ORF 203, has increased recently in wild-type field FWPV isolates. Therefore, it is critical to detect ILTV, FWPV, REV-integrated FWPV, and REV early and accurately. In this study, we successfully developed a multiplex PCR assay for the simultaneous detection of ILTV, FWPV, REV-integrated FWPV, and REV, and the detection limits was 1 × 54 copies/tube. When used to test clinical samples, the results of the multiplex PCR were in 100% agreement with singleplex PCRs and sequencing. This new multiplex PCR is a simple, rapid, sensitive, specific, and cost-effective method for detection of 4 viruses in clinical specimens.

We performed viral metagenomics analysis of Japanese quail affected with enteritis to elucidate the viral etiology. Metagenomics generated 21,066,442 sequence reads via high-throughput sequencing, with a mean length of 136 nt. Enrichment in viral sequences suggested that at least three viruses were present in quail samples. Coronavirus and picornavirus were identified and are known as pathogens causing quail enteritis that match the observed morphology. Abundant reads of coronavirus from quail samples yielded four fragment sequences exhibiting six genomes of avian coronavirus. Sequence analysis showed that this quail coronavirus was related to turkey coronavirus and chicken infectious bronchitis virus. Quail picornavirus 8177 bp in size was identified and was similar to the QPV1/HUN/01 virus detected in quails without clinical symptoms in Hungary with 84.6% nucleotide and 94.6% amino acid identity. Our results are useful for understanding the genetic diversity of quail viruses. Further studies must be performed to determine whether quail coronavirus and quail picornavirus are pathogens of the digestive tract of quails.

The diagnosis of cancer brings about significant changes in the life of a child. In addition to physical pain, pediatric patients face psychological and social challenges. At the same time, some patients also have positive experiences with and attitudes toward their illness and treatment. Drawing on 19 semi-structured interviews with pairs of pediatric cancer patients and their parental caregivers, we examined patients' perspectives on their experience of living with cancer. We identified four salient themes in patients' positive experiences: future-oriented thinking, developing strong personal bonds and relationships, gaining knowledge and life experience, and developing self-management and coping skills. Collectively, the patients' positive experiences indicate that they adapt to their new lives through an evolving process. Based on this process, we provide design implications for health technologies to support and promote positive experiences during illness and treatment.