1. Antibacterial, antioxidant and cytotoxic activities of different fractions of acetone extract from flowers of Dipterocarpus intricatus Dyer (Dipterocarpaceae)
- Author
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Hong Thia Le, Van Son Le, Thien Hong Van, Hoang Dung Nguyen, Dang HoaiTrang Nguyen, Ngọc Nam Trịnh, Huynh Mai Thu Nguyen, Pham Tan Quoc Le, and Thao Nguyen Luu
- Subjects
Dipterocarpaceae ,Antioxidant ,Ecology ,biology ,Traditional medicine ,medicine.medical_treatment ,Plant Science ,biology.organism_classification ,Biochemistry, Genetics and Molecular Biology (miscellaneous) ,chemistry.chemical_compound ,Dipterocarpus intricatus ,chemistry ,Acetone ,medicine ,Cytotoxic T cell ,Ecology, Evolution, Behavior and Systematics - Abstract
This study has shown for the first time the antimicrobial, antioxidant and cytotoxicity of 3 fractions of acetone extract, including hexane, chloroform and ethyl acetate from flowers of Dipterocarpus intricatus. Antibacterial test using disc diffusion method showed that the chloroform and ethyl acetate fractions inhibited the growth of all the tested bacteria, including Escherichia coli, Pseudomonas aeruginosa, Salmonella enteritidis, Salmonella typhimurium, Bacillus cereus and Staphylococcus aureus while the hexane fraction showed the antibacterial activity against B. cereus and S. enteritidis. Antioxidant activity and cancer cell resistance of those extracts were conducted using DPPH and MTT methods respectively. As a result, the DPPH radical scavenging activity of the hexane, chloroform and ethyl acetate fractions were determined with the IC50 values of 0.508, 0.22 and 0.075 mg/mL respectively while the cytotoxicity to HepG2 cell line of those fractions was 163.3 ppm, 106.7 ppm and 459.3 ppm. These results suggested the potential application of these fractions isolated from D. intricatus flowers as the natural antimicrobial, antioxidant and cytotoxic agents for medicine.
- Published
- 2021