1. Quality and Probiotic Lactic Acid Bacteria Diversity of Rabbit Meat Bekasam-Fermented Meat
- Author
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Kusmajadi Suradi, Husmy Yurmiati, Eka Wulandari, and Toto Subroto
- Subjects
Salmonella ,medicine.disease_cause ,Article ,law.invention ,03 medical and health sciences ,Probiotic ,chemistry.chemical_compound ,0302 clinical medicine ,fermented meat ,law ,medicine ,Food science ,Lactobacillus buchneri ,bekasam ,biology ,Chemistry ,microbiology ,0402 animal and dairy science ,04 agricultural and veterinary sciences ,16S ribosomal RNA ,biology.organism_classification ,040201 dairy & animal science ,Yeast ,Lactic acid ,rabbit meat ,030221 ophthalmology & optometry ,Animal Science and Zoology ,Fermentation ,Bacteria ,probiotic ,Food Science - Abstract
Rabbit meat bekasam is a traditional fermentation product from Indonesia. This study aimed to determine the chemical and microbiological characteristics of rabbit meat bekasam during the fermentation process in order to isolate, characterize (in vitro and in vivo), and identify lactic acid bacteria (LAB) as the probiotic candidate. The chemical contents of bekasam on 7-day fermentation were investigated in explorative and experimental methods in a completely randomized design. A proximate analysis reported a decrease in the moisture content, fat and carbohydrate content, and an increase in protein content. Also, lactic acid content was increased from 0.48% to 1.12%, and pH was decreased from 5.3 to 4.3. Other properties indicated different values, such as bacteria (2.75×106 to 4.45×107 CFU/g), total LAB (3.82×106 to 4.67×108 CFU/g), total yeast (9.89×106 to 3.82×108 CFU/g) and total mould (4.34×101 to 4.86×103 CFU/g). The experiment produced nine LAB isolates, including two probiotics subjected to further 16S rRNA gene analysis, which indicated that Lactobacillus buchneri was the potential probiotic isolate. After being tested on BALB/c mice, L. buchneri could improve the immune system by inhibiting the growth of Coliform and Salmonella.
- Published
- 2020