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198 results on '"Hung, Huey-Shan"'

7. Assessment of the Biocompatibility Ability and Differentiation Capacity of Mesenchymal Stem Cells on Biopolymer/Gold Nanocomposites.

Author

Hung, Huey-Shan, Shen, Chiung-Chyi, Wu, Jyun-Ting, Yueh, Chun-Yu, Yang, Meng-Yin, Yang, Yi-Chin, and Cheng, Wen-Yu

Subjects
*MESENCHYMAL stem cell differentiation, *BIOPOLYMERS, *NANOCOMPOSITE materials, *MESENCHYMAL stem cells, *BIOCOMPATIBILITY, *ATOMIC force microscopy
Abstract

This study assessed the biocompatibility of two types of nanogold composites: fibronectin-gold (FN-Au) and collagen-gold (Col-Au). It consisted of three main parts: surface characterization, in vitro biocompatibility assessments, and animal models. To determine the structural and functional differences between the materials used in this study, atomic force microscopy, Fourier-transform infrared spectroscopy, and ultraviolet-visible spectrophotometry were used to investigate their surface topography and functional groups. The F-actin staining, proliferation, migration, reactive oxygen species generation, platelet activation, and monocyte activation of mesenchymal stem cells (MSCs) cultured on the FN-Au and Col-Au nanocomposites were investigated to determine their biological and cellular behaviors. Additionally, animal biocompatibility experiments measured capsule formation and collagen deposition in female Sprague–Dawley rats. The results showed that MSCs responded better on the FN-Au and Col-AU nanocomposites than on the control (tissue culture polystyrene) or pure substances, attributed to their incorporation of an optimal Au concentration (12.2 ppm), which induced significant surface morphological changes, nano topography cues, and better biocompatibility. Moreover, neuronal, endothelial, bone, and adipose tissues demonstrated better differentiation ability on the FN-Au and Col-Au nanocomposites. Nanocomposites have a crucial role in tissue engineering and even vascular grafts. Finally, MSCs were demonstrated to effectively enhance the stability of the endothelial structure, indicating that they can be applied as promising alternatives to clinics in the future. [ABSTRACT FROM AUTHOR]

Published
2024
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9. Fabrication of Nanostructured PLGA Scaffolds Using Anodic Aluminum Oxide Templates

Author

Hsueh, Cheng-Chih, Wang, Gou-Jen, Hsu, Shan-Hui, and Hung, Huey-Shan

Subjects
Computer Science - Other Computer Science
Abstract

PLGA (poly(lactic-co-glycolic acid)) is one of the most used biodegradable and biocompatible materials. Nanostructured PLGA even has great application potentials in tissue engineering. In this research, a fabrication technique for nanostructured PLGA membrane was investigated and developed. In this novel fabrication approach, an anodic aluminum oxide (AAO) film was use as the template ; the PLGA solution was then cast on it ; the vacuum air-extraction process was applied to transfer the nano porous pattern from the AAO membrane to the PLGA membrane and form nanostures on it. The cell culture experiments of the bovine endothelial cells demonstrated that the nanostructured PLGA membrane can double the cell growing rate. Compared to the conventional chemical-etching process, the physical fabrication method proposed in this research not only is simpler but also does not alter the characteristics of the PLGA. The nanostructure of the PLGA membrane can be well controlled by the AAO temperate., Comment: Submitted on behalf of EDA Publishing Association (http://irevues.inist.fr/handle/2042/16838)

Published
2008

19. Correction: Hung et al. Anti-Inflammatory Fibronectin-AgNP for Regulation of Biological Performance and Endothelial Differentiation Ability of Mesenchymal Stem Cells. Int. J. Mol. Sci. 2021, 22, 9262

Author

Hung, Huey-Shan, primary, Chang, Kai-Bo, additional, Tang, Cheng-Ming, additional, Ku, Tian-Ren, additional, Kung, Mei-Lang, additional, Yu, Alex Yang-Hao, additional, Shen, Chiung-Chyi, additional, Yang, Yi-Chin, additional, Hsieh, Hsien-Hsu, additional, and Hsu, Shan-hui, additional

Published
2022
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20. Corrigendum to Functionalized collagen-silver nanocomposites for evaluation of the biocompatibility and vascular differentiation capacity of mesenchymal stem cells Volume 624 (2021), 126814

Author

Chang, Kai-Bo, primary, Shen, Chiung-Chyi, additional, Hsu, Shan-hui, additional, Tang, Chang Ming, additional, Yang, Yi-Chin, additional, Liu, Sin-Ying, additional, Ku, Tian Ren, additional, Kung, Mei-Lang, additional, Hsieh, Hsien-Hsu, additional, and Hung, Huey-Shan, additional

Published
2022
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23. Improved Delivery Performance of n-Butylidenephthalide-Polyethylene Glycol-Gold Nanoparticles Efficient for Enhanced Anti-Cancer Activity in Brain Tumor

Author

Hsing, Ming-Tai, primary, Hsu, Hui-Ting, additional, Chang, Chih-Hsuan, additional, Chang, Kai-Bo, additional, Cheng, Chun-Yuan, additional, Lee, Jae-Hwan, additional, Huang, Chien-Li, additional, Yang, Meng-Yin, additional, Yang, Yi-Chin, additional, Liu, Szu-Yuan, additional, Yen, Chun-Ming, additional, Yang, Shun-Fa, additional, and Hung, Huey-Shan, additional

Published
2022
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28. Favorable Biological Performance Regarding the Interaction between Gold Nanoparticles and Mesenchymal Stem Cells.

Author

Lin, Ruei-Hong, Lee, Hsu-Tung, Yeh, Chun-An, Yang, Yi-Chin, Shen, Chiung-Chyi, Chang, Kai-Bo, Liu, Bai-Shuan, Hsieh, Hsien-Hsu, Wang, Hui-Min David, and Hung, Huey-Shan

Subjects
GOLD nanoparticles, MESENCHYMAL stem cells, NANOMEDICINE, SCANNING electron microscopes, MATRIX metalloproteinases, REACTIVE oxygen species, CELL populations
Abstract

Gold nanoparticles (AuNPs) are well known to interact with cells, leading to different cell behaviors such as cell proliferation and differentiation capacity. Biocompatibility and biological functions enhanced by nanomedicine are the most concerning factors in clinical approaches. In the present research, AuNP solutions were prepared at concentrations of 1.25, 2.5, 5 and 10 ppm for biocompatibility investigations. Ultraviolet–visible spectroscopy was applied to identify the presence of AuNPs under the various concentrations. Dynamic Light Scattering assay was used for the characterization of the size of the AuNPs. The shape of the AuNPs was observed through a Scanning Electron Microscope. Afterward, the mesenchymal stem cells (MSCs) were treated with a differentiation concentration of AuNP solutions in order to measure the biocompatibility of the nanoparticles. Our results demonstrate that AuNPs at 1.25 and 2.5 ppm could significantly enhance MSC proliferation, decrease reactive oxygen species (ROS) generation and attenuate platelet/monocyte activation. Furthermore, the MSC morphology was observed in the presence of filopodia and lamellipodia while being incubated with 1.25 and 2.5 ppm AuNPs, indicating that the adhesion ability was enhanced by the nanoparticles. The expression of matrix metalloproteinase (MMP-2/9) in MSCs was found to be more highly expressed under 1.25 and 2.5 ppm AuNP treatment, relating to better cell migrating ability. Additionally, the cell apoptosis of MSCs investigated with Annexin-V/PI double staining assay and the Fluorescence Activated Cell Sorting (FACS) method demonstrated the lower population of apoptotic cells in 1.25 and 2.5 ppm AuNP treatments, as compared to high concentrations of AuNPs. Additionally, results from a Western blotting assay explored the possibility that the anti-apoptotic proteins Cyclin-D1 and Bcl-2 were remarkably expressed. Meanwhile, real-time PCR analysis demonstrated that the 1.25 and 2.5 ppm AuNP solutions induced a lower expression of inflammatory cytokines (TNF-α, IL-1β, IFN-γ, IL-6 and IL-8). According to the tests performed on an animal model, AuNP 1.25 and 2.5 ppm treatments exhibited the better biocompatibility performance, including anti-inflammation and endothelialization. In brief, 1.25 and 2.5 ppm of AuNP solution was verified to strengthen the biological functions of MSCs, and thus suggests that AuNPs become the biocompatibility nanomedicine for regeneration research. [ABSTRACT FROM AUTHOR]

Published
2023
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32. Delivery Capacity and Anticancer Ability of the Berberine-Loaded Gold Nanoparticles to Promote the Apoptosis Effect in Breast Cancer

Author

Chiu, Chen-Feng, primary, Fu, Ru-Huei, additional, Hsu, Shan-hui, additional, Yu, Yang-Hao (Alex), additional, Yang, Shun-Fa, additional, Tsao, Thomas Chang-Yao, additional, Chang, Kai-Bo, additional, Yeh, Chun-An, additional, Tang, Cheng-Ming, additional, Huang, Sheng-Chu, additional, and Hung, Huey-Shan, additional

Published
2021
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33. Peiminine Reduces ARTS-Mediated Degradation of XIAP by Modulating the PINK1/Parkin Pathway to Ameliorate 6-Hydroxydopamine Toxicity and α-Synuclein Accumulation in Parkinson’s Disease Models In Vivo and In Vitro

Author

Hsu, Yu-Ling, primary, Hung, Huey-Shan, additional, Tsai, Chia-Wen, additional, Liu, Shih-Ping, additional, Chiang, Yu-Ting, additional, Kuo, Yun-Hua, additional, Shyu, Woei-Cherng, additional, Lin, Shinn-Zong, additional, and Fu, Ru-Huei, additional

Published
2021
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40. Maackiain Ameliorates 6-Hydroxydopamine and SNCA Pathologies by Modulating the PINK1/Parkin Pathway in Models of Parkinson’s Disease in Caenorhabditis elegans and the SH-SY5Y Cell Line

Author

Tsai, Rong-Tzong, primary, Tsai, Chia-Wen, additional, Liu, Shih-Ping, additional, Gao, Jia-Xin, additional, Kuo, Yun-Hua, additional, Chao, Pei-Min, additional, Hung, Huey-Shan, additional, Shyu, Woei-Cherng, additional, Lin, Shinn-Zong, additional, and Fu, Ru-Huei, additional

Published
2020
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49. Irisflorentin Modifies Properties of Mouse Bone Marrow-Derived Dendritic Cells and Reduces the Allergic Contact Hypersensitivity Responses

Author

Fu, Ru-Huei, primary, Tsai, Chia-Wen, additional, Tsai, Rong-Tzong, additional, Liu, Shih-Ping, additional, Chan, Tzu-Min, additional, Ho, Yu-Chen, additional, Lin, Hsin-Lien, additional, Chen, Yue-Mi, additional, Hung, Huey-Shan, additional, Chiu, Shao-Chih, additional, Tsai, Chang-Hai, additional, Wang, Yu-Chi, additional, Shyu, Woei-Cherng, additional, and Lin, Shinn-Zong, additional

Published
2015
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