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15 results on '"Hunault, Lise"'

4. Clostridioides difficilebinary toxin CDT induces biofilm-like persisting microcolonies

Author

Meza Torres, Jazmin, primary, Tinevez, Jean-Yves, additional, Crozouls, Aline, additional, Mary, Héloïse, additional, Kim, Minhee, additional, Hunault, Lise, additional, Chamorro-Rodriguez, Susan, additional, Lejal, Emilie, additional, Altamirano-Silva, Pamela, additional, Gobaa, Samy, additional, Peltier, Johann, additional, Chassaing, Benoit, additional, and Dupuy, Bruno, additional

Published
2024
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5. Rocuronium-specific antibodies drive perioperative anaphylaxis but can also function as reversal agents in preclinical models.

Author

Dejoux, Alice, Zhu, Qianqian, Ganneau, Christelle, Goff, Odile Richard-Le, Godon, Ophélie, Lemaitre, Julien, Relouzat, Francis, Huetz, François, Sokal, Aurélien, Vandenberghe, Alexis, Pecalvel, Cyprien, Hunault, Lise, Derenne, Thomas, Gillis, Caitlin M., Iannascoli, Bruno, Wang, Yidan, Rose, Thierry, Mertens, Christel, Nicaise-Roland, Pascale, and England, Patrick

Subjects
IMMUNOGLOBULIN E, IMMUNOLOGIC memory, NEUROMUSCULAR blocking agents, NEUROMUSCULAR blockade, MAST cells
Abstract

Neuromuscular blocking agents (NMBAs) relax skeletal muscles to facilitate surgeries and ease intubation but can lead to adverse reactions, including complications because of postoperative residual neuromuscular blockade (rNMB) and, in rare cases, anaphylaxis. Both adverse reactions vary between types of NMBAs, with rocuronium, a widely used nondepolarizing NMBA, inducing one of the longest rNMB durations and highest anaphylaxis incidences. rNMB induced by rocuronium can be reversed by the synthetic γ-cyclodextrin sugammadex. However, in rare cases, sugammadex can provoke anaphylaxis. Thus, additional therapeutic options are needed. Rocuronium-induced anaphylaxis is proposed to rely on preexisting rocuronium-binding antibodies. To understand the pathogenesis of rocuronium-induced anaphylaxis and to identify potential therapeutics, we investigated the memory B cell antibody repertoire of patients with suspected hypersensitivity to rocuronium. We identified polyclonal antibody repertoires with a high diversity among V(D)J genes without evidence of clonal groups. When recombinantly expressed, these antibodies demonstrated specificity and low affinity for rocuronium without cross-reactivity for other NMBAs. Moreover, when these antibodies were expressed as human immunoglobulin E (IgE), they triggered human mast cell activation and passive systemic anaphylaxis in transgenic mice, although their affinities were insufficient to serve as reversal agents. Rocuronium-specific, high-affinity antibodies were thus isolated from rocuronium-immunized mice. The highest-affinity antibody was able to reverse rocuronium-induced neuromuscular blockade in nonhuman primates with kinetics comparable to that of sugammadex. Together, these data support the hypothesis that antibodies cause anaphylactic reactions to rocuronium and pave the way for improved diagnostics and neuromuscular blockade reversal agents. Editor's summary: Neuromuscular blocking agents (NMBAs) such as rocuronium are used during anesthesia to assist with surgeries and intubations. Although these agents are effective, they are not without risk. Patients can develop postoperative residual neuromuscular blockade, which can be treated with an NMBA reversal agent, and life-threatening anaphylaxis can occur in rare cases. Here, Dejoux et al. isolated and characterized memory B cells from three individuals who developed reactions to NMBA treatment and had serum antibodies to rocuronium. The authors found that IgE antibodies generated from these memory B cells were sufficient to induce anaphylaxis in mice treated with rocuronium, although they were too low affinity to be used as reversal agents. This led the authors to immunize mice with rocuronium to generate antibodies of higher affinity, which were able to reverse neuromuscular blockade in rocuronium-treated nonhuman primates. Together, these data explain why these three patients had a reaction and offer a therapeutic approach to reversing neuromuscular blockade. —Courtney Malo [ABSTRACT FROM AUTHOR]

Published
2024
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6. Anti-S-layer monoclonal antibodies impact Clostridioides difficile physiology

Author

Hunault, Lise, primary, Auria, Emile, additional, England, Patrick, additional, Deschamps, Julien, additional, Briandet, Romain, additional, Kremer, Vanessa, additional, Iannascoli, Bruno, additional, Vidal-Maison, Léo, additional, Guo, Chunguang, additional, Macdonald, Lynn, additional, Péchiné, Séverine, additional, Denève-Larrazet, Cécile, additional, Dupuy, Bruno, additional, Gorochov, Guy, additional, Bruhns, Pierre, additional, and Sterlin, Delphine, additional

Published
2024
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8. Anti-S-layer monoclonal antibodies impactC. difficilephysiology

Author

Hunault, Lise, primary, Auria, Emile, additional, England, Patrick, additional, Deschamps, Julien, additional, Briandet, Romain, additional, Kremer, Vanessa, additional, Iannascoli, Bruno, additional, Vidal-Maison, Léo, additional, Guo, Chunguang, additional, Macdonald, Lynn, additional, Péchiné, Séverine, additional, Denève-Larrazet, Cécile, additional, Dupuy, Bruno, additional, Gorochov, Guy, additional, Bruhns, Pierre, additional, and Sterlin, Delphine, additional

Published
2023
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9. A monoclonal antibody collection forC. difficiletyping

Author

Hunault, Lise, primary, England, Patrick, additional, Barbut, Frédéric, additional, Iannascoli, Bruno, additional, Godon, Ophélie, additional, Déjardin, François, additional, Thomas, Christophe, additional, Dupuy, Bruno, additional, Guo, Chunguang, additional, Macdonald, Lynn, additional, Gorochov, Guy, additional, Sterlin, Delphine, additional, and Bruhns, Pierre, additional

Published
2023
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10. SARS-CoV-2 Omicron BA.1 breakthrough infection drives late remodeling of the memory B cell repertoire in vaccinated individuals

Author

Sokal, Aurélien, primary, Barba-Spaeth, Giovanna, additional, Hunault, Lise, additional, Fernández, Ignacio, additional, Broketa, Matteo, additional, Meola, Annalisa, additional, Fourati, Slim, additional, Azzaoui, Imane, additional, Vandenberghe, Alexis, additional, Lagouge-Roussey, Pauline, additional, Broutin, Manon, additional, Roeser, Anais, additional, Bouvier-Alias, Magali, additional, Crickx, Etienne, additional, Languille, Laetitia, additional, Fournier, Morgane, additional, Michel, Marc, additional, Godeau, Bertrand, additional, Gallien, Sébastien, additional, Melica, Giovanna, additional, Nguyen, Yann, additional, Canoui-Poitrine, Florence, additional, Pirenne, France, additional, Megret, Jérôme, additional, Pawlotsky, Jean-Michel, additional, Fillatreau, Simon, additional, Reynaud, Claude-Agnès, additional, Weill, Jean-Claude, additional, Rey, Félix A., additional, Bruhns, Pierre, additional, Mahévas, Matthieu, additional, and Chappert, Pascal, additional

Published
2023
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11. Omicron BA.1 breakthrough infection drives long-term remodeling of the memory B cell repertoire in vaccinated individuals

Author

Sokal, Aurélien, primary, Barba-Spaeth, Giovanna, additional, Hunault, Lise, additional, Fernández, Ignacio, additional, Broketa, Matteo, additional, Meola, Annalisa, additional, Fourati, Slim, additional, Azzaoui, Imane, additional, Vandenberghe, Alexis, additional, Lagouge-Roussey, Pauline, additional, Broutin, Manon, additional, Roeser, Anais, additional, Bouvier-Alias, Magali, additional, Crickx, Etienne, additional, Languille, Laetitia, additional, Fournier, Morgane, additional, Michel, Marc, additional, Godeau, Bertrand, additional, Gallien, Sébastien, additional, Melica, Giovanna, additional, Nguyen, Yann, additional, Canoui-Poitrine, Florence, additional, Noizat-Pirenne, France, additional, Megret, Jérôme, additional, Pawlotsky, Jean-Michel, additional, Fillatreau, Simon, additional, Reynaud, Claude-Agnès, additional, Weill, Jean-Claude, additional, Rey, Félix A., additional, Bruhns, Pierre, additional, Mahévas, Matthieu, additional, and Chappert, Pascal, additional

Published
2023
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12. Omicron BA.1 breakthrough infection drives long-term remodeling of the memory B cell repertoire in vaccinated individuals

Author

Sokal, Aurélien, Barba-Spaeth, Giovanna, Hunault, Lise, Fernández, Ignacio, Broketa, Matteo, Meola, Annalisa, Fourati, Slim, Azzaoui, Imane, Vandenberghe, Alexis, Lagouge-Roussey, Pauline, Broutin, Manon, Roeser, Anais, Bouvier-Alias, Magali, Crickx, Etienne, Languille, Laetitia, Fournier, Morgane, Michel, Marc, Godeau, Bertrand, Gallien, Sébastien, Melica, Giovanna, Nguyen, Yann, Canoui-Poitrine, Florence, Noizat-Pirenne, France, Megret, Jérôme, Pawlotsky, Jean-Michel, Fillatreau, Simon, Reynaud, Claude-Agnès, Weill, Jean-Claude, Rey, Félix, Bruhns, Pierre, Mahévas, Matthieu, Chappert, Pascal, Institut Necker Enfants-Malades (INEM - UM 111 (UMR 8253 / U1151)), Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Université Paris Cité (UPCité), Hôpital Beaujon [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), CIC - CHU Henri Mondor, Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Paris-Est Créteil Val-de-Marne - Paris 12 (UPEC UP12), Virologie Structurale - Structural Virology, Institut Pasteur [Paris] (IP)-Centre National de la Recherche Scientifique (CNRS)-Université Paris Cité (UPCité), Anticorps en thérapie et pathologie - Antibodies in Therapy and Pathology, Institut Pasteur [Paris] (IP)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Paris Cité (UPCité), Physiologie, physiopathologie et thérapeutique (ED 394), Sorbonne Université (SU), Centre d'Immunologie et des Maladies Infectieuses (CIMI), Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS), CHU Henri Mondor [Créteil], Institut Mondor de Recherche Biomédicale (IMRB), Institut National de la Santé et de la Recherche Médicale (INSERM)-IFR10-Université Paris-Est Créteil Val-de-Marne - Paris 12 (UPEC UP12), Hôpital Henri Mondor, Université Paris-Est Créteil Val-de-Marne - Paris 12 (UPEC UP12), Hôpital Cochin [AP-HP], IMRB - CEPIA/'Clinical Epidemiology And Ageing : Geriatrics, Primary Care and Public Health' [Créteil] (U955 Inserm - UPEC), Institut National de la Santé et de la Recherche Médicale (INSERM)-IFR10-Université Paris-Est Créteil Val-de-Marne - Paris 12 (UPEC UP12)-Institut National de la Santé et de la Recherche Médicale (INSERM)-IFR10-Université Paris-Est Créteil Val-de-Marne - Paris 12 (UPEC UP12), Etablissement Français du Sang [Créteil], Structure Fédérative de Recherche Necker (SFR Necker - UMS 3633 / US24), Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Université Paris Cité (UPCité), and This work was funded by the CAPNET (Comité ad-hoc de pilotage national des essaisthérapeutiques et autres recherches, French government) and by the Fondation PrincesseGrace. Assistance Publique – Hôpitaux de Paris (AP-HP, Département de la Recherche Cli-nique et du Développement) was the promotor and sponsor of MEMO-COV-2. Work in theUnit of Structural Virology was funded by Institut Pasteur, Urgence COVID-19 FundraisingCampaign of Institut Pasteur. PB acknowledges funding from the Institut Pasteur and InsitutNational de la Santé et de la Recherche Médicale (INSERM). AS was supported by a Posted’accueil from INSERM.

Subjects
[SDV]Life Sciences [q-bio], [SDV.IMM]Life Sciences [q-bio]/Immunology
Abstract

SummaryHow infection by a viral variant showing antigenic drift impacts a preformed mature human memory B cell (MBC) repertoire remains an open question. Here, we studied the MBC response up to 6 months after Omicron BA.1 breakthrough infection in individuals previously vaccinated with three doses of mRNA vaccine. Longitudinal analysis, using single-cell multi-omics and functional analysis of monoclonal antibodies from RBD-specific MBCs, revealed that a BA.1 breakthrough infection mostly recruited pre-existing cross-reactive MBCs with limitedde novoresponse against BA.1-restricted epitopes. Reorganization of clonal hierarchy and new rounds of germinal center reaction, however, combined to maintain diversity and induce progressive maturation of the MBC repertoire against common Hu-1 and BA.1, but not BA.5-restricted, SARS-CoV-2 Spike RBD epitopes. Such remodeling was further associated with marked improvement in overall neutralizing breadth and potency. These findings have fundamental implications for the design of future vaccination booster strategies.

Published
2023
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15. Detection of TET-Oxidized 5-Methylcytosine Bases by Capillary Gel Electrophoresis.

Author

Vaisvila R, Hunault L, and Saleh L

Subjects
Data Analysis, Enzyme Activation, Humans, Oligonucleotides chemistry, Oligonucleotides metabolism, Oxidation-Reduction, 5-Methylcytosine chemistry, Electrophoresis, Capillary methods, Mixed Function Oxygenases metabolism
Abstract

The oxidation activity of the mammalian ten-eleven translocation dioxygenase (TET) on 5-methylcytosine (5mC) of DNA is usually monitored by analytical methods such as dot blotting and liquid chromatography-mass spectrometry (LC-MS). Herein, we describe a high throughput capillary gel electrophoresis assay for monitoring the in vitro oxidation of 5mC by TET. The method is rapid and quantitative, and can serve as a powerful tool in mechanistic studies of TET.

Published
2021
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