1. Meiotic susceptibility for induction of sperm with chromosomal aberrations in patients receiving combination chemotherapy for Hodgkin lymphoma.
- Author
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Frias, Sara, Van Hummelen, Paul, Meistrich, Marvin L, and Wyrobek, Andrew J
- Subjects
Testis ,Spermatozoa ,Humans ,Hodgkin Disease ,Chromosome Aberrations ,Mitoxantrone ,Vinblastine ,Vincristine ,Prednisone ,Antineoplastic Combined Chemotherapy Protocols ,Radiotherapy Dosage ,In Situ Hybridization ,Fluorescence ,Cohort Studies ,Meiosis ,Spermatogenesis ,Mutagenesis ,Time Factors ,Adult ,Male ,Semen Analysis ,Organs at Risk ,Chemoradiotherapy ,Organ Sparing Treatments ,Fertility Preservation ,Adult Germline Stem Cells ,Cancer Survivors ,In Situ Hybridization ,Fluorescence ,General Science & Technology - Abstract
Improvements in survival rates with gonad-sparing protocols for childhood and adolescence cancer have increased the optimism of survivors to become parents after treatment. Findings in rodents indicate that chromosomal aberrations can be induced in male germ cells by genotoxic exposures and transmitted to offspring and future generations with effects on development, fertility and health. Thus, there is a need for effective technologies to identify human sperm carrying chromosomal aberrations to assess the germ-line risks, especially for cancer survivors who have received genotoxic therapies. The time-dependent changes in the burden of sperm carrying structural chromosomal aberrations were assessed for the first time in a cancer setting, using the AM8 sperm FISH protocol which simultaneously detects abnormalities in chromosomal structure and number in sperm. Nine Hodgkin lymphoma (HL) patients provided 20 semen samples before, during, and after NOVP therapy (Novantrone, Oncovin, Velban and Prednisone) and radiation therapy that produced scattered gonadal doses from
- Published
- 2020