31 results on '"Humberto Aguirre-Becerra"'
Search Results
2. Preliminary Screening on Antibacterial Crude Secondary Metabolites Extracted from Bacterial Symbionts and Identification of Functional Bioactive Compounds by FTIR, HPLC and Gas Chromatography–Mass Spectrometry
- Author
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Gobinath Chandrakasan, Juan Fernando García-Trejo, Ana Angelica Feregrino-Pérez, Humberto Aguirre-Becerra, Enrique Rico García, and María Isabel Nieto-Ramírez
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entomopathogenic nematodes ,symbiotic bacteria ,PCR ,antibacterial activity ,HPLC ,GC–MS ,Organic chemistry ,QD241-441 - Abstract
Secondary metabolites, bioactive compounds produced by living organisms, can unveil symbiotic relationships in nature. In this study, soilborne entomopathogenic nematodes associated with symbiotic bacteria (Xenorhabdus stockiae and Photorhabdus luminescens) were extracted from solvent supernatant containing secondary metabolites, demonstrating significant inhibitory effects against E. coli, S. aureus, B. subtilus, P. mirabilis, E. faecalis, and P. stutzeri. The characterization of these secondary metabolites by Fourier transforms infrared spectroscopy revealed amine groups of proteins, hydroxyl and carboxyl groups of polyphenols, hydroxyl groups of polysaccharides, and carboxyl groups of organic acids. Furthermore, the obtained crude extracts were analyzed by high-performance liquid chromatography for the basic identification of potential bioactive peptides. Gas chromatography–mass spectrometry analysis of ethyl acetate extracts from Xenorhabdus stockiae identified major compounds including nonanoic acid derivatives, proline, paromycin, octodecanal derivatives, trioxa-5-aza-1-silabicyclo, 4-octadecenal, methyl ester, oleic acid, and 1,2-benzenedicarboxylicacid. Additional extraction from Photorhabdus luminescens yielded functional compounds such as indole-3-acetic acid, phthalic acid, 1-tetradecanol, nemorosonol, 1-eicosanol, and unsaturated fatty acids. These findings support the potential development of novel natural antimicrobial agents for future pathogen suppression.
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- 2024
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3. Bioactive compounds and biological activity in edible insects: A review
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María de la Luz Sánchez-Estrada, Humberto Aguirre-Becerra, and Ana Angélica Feregrino-Pérez
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Edible insects ,Biological activities ,Nutritional aspects ,Anti-nutritional aspects ,Science (General) ,Q1-390 ,Social sciences (General) ,H1-99 - Abstract
New strategies to combat hunger are a current and urgent demand. The increase in population has generated a high demand for products and services that affect food production, cultivation areas, and climate. Viable and sustainable alternative sources have been sought to meet food quality requirements. In this context, edible insects are a good source of macro-nutrients, and bioactive compounds confer biological properties that improve their nutritional aspects and benefit human health. This review aims to present the benefits and contributions of edible insects from the point of view of the biological contribution of macronutrients, and bioactive compounds, as well as consider some anti-nutritional aspects reported in edible insects. It was found that insects possess most of the macronutrients necessary for human life and are rich in bioactive compounds commonly found in plants. These bioactive compounds can vary significantly depending on the developmental stage, diet, and species of edible insects. However, they also contain phytochemicals in which anti-nutrients predominate, which can adversely affect humans with allergenic reactions or reduced nutrient viability when consumed in high amounts or for prolonged periods. Hydrocyanide, oxalates, soluble oxalate, and phytate are the most studied anti-nutrients. However, the doses at which they occur are far below the limits in foods. In addition, anti-nutrient levels decrease significantly in processing, such as oven-drying and defatting methods. However, there are few studies, so more trials are needed to avoid generalizing. Therefore, edible insects can be considered complete food.
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- 2024
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4. Bioactive Compounds from Organic Waste
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Benito Parra-Pacheco, Byanka A. Cruz-Moreno, Humberto Aguirre-Becerra, Juan Fernando García-Trejo, and Ana Angélica Feregrino-Pérez
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organic waste ,bioactive compounds ,biological properties ,added value ,waste revaluation ,Organic chemistry ,QD241-441 - Abstract
The reuse and reincorporation of waste are the principles of circular economies. Compost, biofuels, animal feed, dyes, and bioactive compounds can be obtained from the revaluation of organic waste. Research on this subject is scarce and limited to specific sectors, such as agriculture and agroindustry, leaving aside others that generate large quantities of organic waste, such as floriculture. The remains of these sectors have a low decomposition rate compared to other organic wastes. They are a source of bioactive compounds (e.g., essential oils, pigments, phenols) that can be reincorporated into the production chain of various industries. This review describes the composition of waste from agroindustry, agriculture, and floriculture, analyzing their potential revalorization as a source of bioactive compounds and an alternative supply source.
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- 2024
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5. Fish Responses to Alternative Feeding Ingredients under Abiotic Chronic Stress
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Julieta Sánchez-Velázquez, Guillermo Abraham Peña-Herrejón, and Humberto Aguirre-Becerra
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alternative feeding ,chronic stress ,cortisol ,environmental stressors ,animal welfare ,Veterinary medicine ,SF600-1100 ,Zoology ,QL1-991 - Abstract
Aquaculture has become one of the most attractive food production activities as it provides high-quality protein for the growing human population. However, the abiotic chronic stress of fish in intensive fish farming leads to a detrimental condition that affects their health and somatic growth, comprising productive performance. This work aims to comprehensively review the impact of alternative and novel dietary protein sources on fish somatic growth, metabolism, and antioxidative capacity under environmental/abiotic stressors. The documental research indicates that ingredients from rendered animal by-products, insects, bacteria as single-cell proteins, and fungal organisms (e.g., yeast, filamentous fungus, and mushrooms) benefit fish health and performance. A set of responses allows fish growth, health, and survival to remain unaffected by feeding with alternative ingredients during chronic environmental stress. Those ingredients stimulate the production of enzymes such as catalase, glutathione peroxidase, and selenoproteins that counteract ROS effects. In addition, the humoral immune system promotes immunoglobulin production (IgM) and cortisol plasmatic reduction. Further investigation must be carried out to establish the specific effect by species. Additionally, the mixture and the pre-treatment of ingredients such as hydrolysates, solid fermentations, and metabolite extraction potentialize the beneficial effects of diets in chronically stressed fish.
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- 2024
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6. Bioactive Compounds of Endemic Medicinal Plants (Cuphea spp.) Cultured in Aquaponic Systems: A Short Study
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Priscila Sarai Flores-Aguilar, Amanda Kim Rico-Chávez, Eloy Rodriguez-deLeón, Humberto Aguirre-Becerra, Sergio Aurelio Zamora-Castro, and Genaro Martín Soto-Zarazúa
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medicinal plants ,aquaponics ,biostimulation ,sustainable food production systems ,secondary metabolites ,Agriculture (General) ,S1-972 - Abstract
Aquaculture waters can be associated with the modification of the phytochemical profile in plants when they are used for irrigation; thus, Integrated Agri-Aquaculture Systems such as aquaponics represent a strategy to improve the bioactive content of medicinal plants. This study aimed to analyze the effect caused by cultivation using aquaponics on the modification of the content of bioactive compounds such as phenols, flavonoids, and apigenin in Cuphea hyssopifolia and Cuphea cyanea irrigated with Cyprinus carpio waters. The results of each culture method showed unique differences (p ≤ 0.05) in the concentrations of bioactive compounds and antioxidant activity in Cuphea spp. For C. hyssopifolia in aquaponics, 76% (61.08 ± 7.2 mg g−1 GAEq) of phenols and 50% (5.62 ± 0.5 mg g−1 CAEq) of flavonoids were maintained compared to 20% (16.99 ± 0.4 mg g−1 GAEq) of phenols and 76.5% (8.19 ± 1.6 mg g−1 CAEq) of flavonoids in conventional culture. For C. cyanea in aquaponics, 91% (15.36 ± 0.8 mg g−1 GAEq) of phenols and 47% (3.52 ± 0.6 mg g−1 CAEq) of flavonoids were maintained compared to 24% (14.11 ± 1.3 mg g−1 GAEq) of phenols and 82% (1.79 ± 0.1 mg g−1 CAEq) of flavonoids in conventional culture. An increase of more than 60% in the apigenin content of C. hyssopifolia in aquaponics confirms a eustress effect related to the use of organically enriched waters. The results indicate that aquaponics can promote the biostimulation/elicitation of medicinal plants and increase their bioactive compounds, but this effect does not occur in the same way between species.
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- 2023
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7. Nanomaterials as an alternative to increase plant resistance to abiotic stresses
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Humberto Aguirre-Becerra, Ana Angélica Feregrino-Pérez, Karen Esquivel, Claudia Elena Perez-Garcia, Ma. Cristina Vazquez-Hernandez, and Aurora Mariana-Alvarado
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nanoparticles ,nanomaterials ,abiotic stress ,plant eustress ,secondary metabolites ,elicitor ,Plant culture ,SB1-1110 - Abstract
The efficient use of natural resources without negative repercussions to the environment has encouraged the incursion of nanotechnology to provide viable alternatives in diverse areas, including crop management. Agriculture faces challenges due to the combination of different abiotic stresses where nanotechnology can contribute with promising applications. In this context, several studies report that the application of nanoparticles and nanomaterials positively affects crop productivity through different strategies such as green synthesis of nanoparticles, plant targeted protection through the application of nanoherbicides and nanofungicides, precise and constant supply of nutrients through nanofertilizers, and tolerance to abiotic stress (e.g., low or high temperatures, drought, salinity, low or high light intensities, UV-B, metals in soil) by several mechanisms such as activation of the antioxidant enzyme system that alleviates oxidative stress. Thus, the present review focuses on the benefits of NPs against these type of stress and their possible action mechanisms derived from the interaction between nanoparticles and plants, and their potential application for improving agricultural practices.
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- 2022
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8. Characterization of the Key Compounds of Bell Pepper by Spectrophotometry and Gas Chromatography on the Effects of Induced Stress on the Concentration of Secondary Metabolite
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Sandra N. Jimenez-García, Lina Garcia-Mier, Xóchitl S. Ramirez-Gomez, Ramon G. Guevara-Gonzalez, Humberto Aguirre-Becerra, Alexandro Escobar-Ortiz, Luis M. Contreras-Medina, Juan F. Garcia-Trejo, Moises A. Vazquez-Cruz, and Ana A. Feregrino-Perez
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sweet pepper ,elicitor ,salicylic acid ,hydrogen peroxide ,electrical conductivity ,bioactive compounds ,Organic chemistry ,QD241-441 - Abstract
Sweet peppers are consumed worldwide, and traditional uses have sparked interest in their applications as dietary antioxidants, which can be enhanced in plants using elicitors. These are endowed with phytochemicals with potential health benefits such as antioxidants, bioavailability, and bioaccessibility. The trend in metabolomics shows us chemical fingerprints linking metabolomics, innovative analytical form, and bioinformatics tools. The objective was to evaluate the impact of multiple stress interactions, elicitor concentrations, and electrical conductivity on the concentration of secondary metabolites to relate their response to metabolic pathways through the foliar application of a cocktail of said elicitors in pepper crops under greenhouse conditions. The extracts were analyzed by spectrophotometry and gas chromatography, and it was shown that the PCA analysis identified phenolic compounds and low molecular weight metabolites, confirming this as a metabolomic fingerprint in the hierarchical analysis. These compounds were also integrated by simultaneous gene and metabolite simulants to obtain effect information on different metabolic pathways. Showing changes in metabolite levels at T6 (36 mM H2O2 and 3.6 dS/m) and T7 (0.1 mM SA and 3.6 dS/m) but showing statistically significant changes at T5 (3.6 dS/m) and T8 (0.1 mM SA, 36 mM H2O2, and 3.6 dS/m) compared to T1 (32 dS/m) or control. Six pathways changed significantly (p < 0.05) in stress-induced treatments: aminoacyl t-RNA and valine-leucine-isoleucine biosynthesis, and alanine-aspartate-glutamate metabolism, glycoxylate-dicarboxylate cycle, arginine-proline, and citrate. This research provided a complete profile for the characterization of metabolomic fingerprint of bell pepper under multiple stress conditions.
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- 2023
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9. Effect of Extended Photoperiod with a Fixed Mixture of Light Wavelengths on Tomato Seedlings
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Humberto Aguirre-Becerra, Juan Fernando García-Trejo, Cristina Vázquez-Hernández, Aurora Mariana Alvarado, Ana Angélica Feregrino-Pérez, Luis Miguel Contreras-Medina, and Ramón G. Guevara-Gonzalez
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seedling ,light wavelength ,extended photoperiod ,photomorphogenesis ,secondary metabolites ,Plant culture ,SB1-1110 - Abstract
Light is an abiotic factor, and its quality, quantity, and photoperiod can be modulated to work as eustress inductors to regulate plant processes. It is known that red (R), blue (B), far-red (FR), and ultraviolet-A wavelengths can promote photomorphogenesis and secondary metabolite production in plants. Several ratios of R:B and the addition of end of-day FR, separately, have beneficial effects on plant development, whereas adding ultraviolet-A enhances the production of secondary metabolites such as phenols. However, the effects of extended photoperiods with a mixture of these four wavelengths and extra end-of-day FR have not been evaluated for plants of commercial interest. The objective of this study was to determine the effects on tomato seedlings (‘Saladette’, CORDOBA F1) of different overnight photoperiods using a fixed combination of R (625 nm), B (460 nm), FR (720 nm), and ultraviolet-A (410 nm). We expected increases in the production of specialized metabolites and the generation of beneficial changes in the seedling biomass and morphology. Four treatments involving overnight artificial light provided by a light-emitting diode (LED) module were established: TC (control), 0 h; T1, 4 hours; T2, 8 hours; and T3, 12 hours. All treatments were subjected to a 12-hour natural photoperiod and 12 hours of overnight artificial light. The experiment lasted 4 weeks, and plants were sampled every week for physical and phytochemical measurements. In general, seedlings subjected to 4-hour and 8-hour treatments presented better results than those subjected to the control and 12-hour overnight photoperiod treatments. Seedlings subjected to treatments with an 8-hour overnight photoperiod presented large accumulation of biomass in the stem rather than in the leaves because they had large stem dry weight, stem weight, and elongation and higher first, second, and third internode lengths; however, they had lower leaf area, leaf dry weight, and health index. Seedlings subjected to treatment with a 4-hour overnight photoperiod were visually bigger, with large leaf expansion, total length, stem weight, total weight, and specific leaf area; however, this treatment had a negative impact on the biomass accumulation, with lower leaf weight, stem dry weight, and health index. The 12-hour treatment had a negative impact on the leaf area, and thus the specific leaf area, of seedlings; however, the biomass accumulation was large, with higher leaf dry weight, total dry matter, and specific leaf area, but no difference in stem dry weight compared with the control. At the end of the experiment, the total phenolic content increased in all treatments compared with the control, but the flavonoid content decreased. Moreover, the antioxidant capacity was higher for T2 during the last 2 weeks of the experiment. Results are discussed according to the possibilities of using this light strategy for seedling production.
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- 2020
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10. Pitahaya Peel: A By-Product with Great Phytochemical Potential, Biological Activity, and Functional Application
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Sandra N. Jimenez-Garcia, Lina Garcia-Mier, Xóchitl S. Ramirez-Gomez, Humberto Aguirre-Becerra, Alexandro Escobar-Ortiz, Luis M. Contreras-Medina, Juan F. Garcia-Trejo, and Ana A. Feregrino-Perez
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phenolic compounds ,biological activity ,Hylocerous spp. ,Organic chemistry ,QD241-441 - Abstract
Hylocereus spp. present two varieties of commercial interest due to their color, organoleptic characteristics, and nutritional contribution, such as Hylocerous polyrhizus and Selenicerus undatus. The fruit recognized as dragon fruit or Pitahaya is an exotic fruit whose pulp is consumed, while the peel is discarded during the process. Studies indicate that the pulp has vitamin C and betalains, and seeds are rich in essential fatty acids, compounds that can contribute to the prevention of chronic non-communicable diseases (cancer, hypertension, and diabetes). In the present study, polyphenolic compounds, biological activity, and fatty acids present in the peel of the two varieties of pitahaya peel were evaluated, showing as a result that the variety S. undatus had higher antioxidant activity with 51% related to the presence of flavonoids 357 mgRE/g sample and fatty acids (hexadecanoic acid and linoleate) with 0.310 and 0.248 mg AG/g sample, respectively. On the other hand, H. polyrhizuun showed a significant difference in the inhibitory activity of amylase and glucosidase enzymes with 68% and 67%, respectively. We conclude that pitahaya peel has potential health effects and demonstrate that methylated fatty acids could be precursors to betalain formation, as well as showing effects against senescence and as a biological control against insects; in the same way, the peel can be reused as a by-product for the extraction of important enzymes in the pharmaceutical and food industry.
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- 2022
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11. Review and Perspectives of the Use of Alginate as a Polymer Matrix for Microorganisms Applied in Agro-Industry
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Betsie Martínez-Cano, Cristian Josué Mendoza-Meneses, Juan Fernando García-Trejo, Gonzalo Macías-Bobadilla, Humberto Aguirre-Becerra, Genaro Martín Soto-Zarazúa, and Ana Angélica Feregrino-Pérez
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sodium alginate ,coating ,encapsulation ,microorganisms ,biocontrol ,plant-growth promotion ,Organic chemistry ,QD241-441 - Abstract
Alginate is a polysaccharide with the property of forming hydrogels, which is economic production, zero toxicity, and biocompatibility. In the agro-industry, alginate is used as a super absorbent polymer, coating seeds, fruits, and vegetables and as a carrier of bacteria and fungi as plant-growth promoters and biocontrol. The latter has a high impact on agriculture since the implementation of microorganisms in a polymer matrix improves soil quality; plant nutrition, and is functional as a preventive measure for the appearance of phytopathogenic. Additionally, it minimizes losses of foods due to wrong post-harvest handling. In this review, we provide an overview of physicochemical properties of alginate, some methods for preparation and modification of capsules and coatings, to finally describe its application in agro-industry as a matrix of plant-growth-promoting microorganisms, its effectiveness in cultivation and post-harvest, and its effect on the environment, as well as the prospects for future agro-industrial applications.
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- 2022
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12. Jacaranda flower (Jacaranda mimosifolia) as an alternative for antioxidant and antimicrobial use
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Humberto Aguirre-Becerra, Silvia Araceli Pineda-Nieto, Juan Fernando García-Trejo, Ramón G. Guevara-González, Ana Angelica Feregrino-Pérez, Beatriz Liliana Álvarez-Mayorga, and Dulce María Rivera Pastrana
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Jacaranda mimosifolia ,Antioxidant ,Antimicrobial ,Phenolic compounds ,Science (General) ,Q1-390 ,Social sciences (General) ,H1-99 - Abstract
Antimicrobial resistance to antibiotics is a serious health problem worldwide, for this reason, the search for natural agents with antimicrobial power against pathogenic microorganisms is of current importance. The objective of this work was to evaluate the antioxidant capacity (ABTS+ and DPPH), antimicrobial activity, and polyphenol compounds of methanolic and aqueous extracts of Jacaranda mimosifolia flowers. The antimicrobial activity against Bacillus cereus ATCC 10876, Bacillus subtilis ATCC 6633, Enterococcus faecalis ATCC 51299, Escherichia coli ATCC 25922, Listeria monocytogenes ATCC 19115, Pseudomonas aeruginosa ATCC 27853, Salmonella typhimurium ATCC 14028, Staphylococcus aureus ATCC 25923, and Streptococcus mutans ATCC 25175, was determined using the Kirby Bauer technique. The results of polyphenolic compounds showed a high amount of total flavonoids in the methanolic and aqueous extracts (503.3 ± 86.5 and 245. 7 ± 27.8 mg Rutin Equivalents/g DW, respectively). Quercetin, gallic acid, caffeic acid, and rutin were identified by the HPLC-DAD technique, while in the GC-MS analysis, esters, fatty acids, organic compounds, as well as monosaccharides were identified. Higher antioxidant capacity was detected by the ABTS technique (94.9% and 62.6%) compared to DPPH values (52.5% and 52.7 %) for methanolic and aqueous extracts, respectively. The methanolic extract showed a greater inhibitory effect on gram-positive bacteria, with a predominant higher inhibition percentage on Listeria monocytogenes and Streptococcus mutans (86% for both). In conclusion, Jacaranda flower extracts could be a natural antimicrobial and antioxidant alternative due to the considerable amount of polyphenolic compounds, and serve as a sustainable alternative for the isolation of active ingredients that could help in agriculture, aquaculture, livestock, pharmaceutics, and other industrial sectors, to remediate problems such as oxidative stress and antimicrobial abuse.
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- 2020
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13. A Simple Methodology to Develop Bifilar, Quadrifilar, and Octofilar Calculable Resistors
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Alepth H. Pacheco-Estrada, Felipe L. Hernandez-Marquez, Carlos D. Aviles, Carlos Duarte-Galvan, Juvenal Rodríguez-Reséndiz, Humberto Aguirre-Becerra, and Luis M. Contreras-Medina
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calculable resistor ,frequency dependence ,impedance metrology ,reference standard ,evanohm heat-treatment ,Technology ,Engineering (General). Civil engineering (General) ,TA1-2040 ,Biology (General) ,QH301-705.5 ,Physics ,QC1-999 ,Chemistry ,QD1-999 - Abstract
This paper describes the development of bifilar, quadrifilar, and octofilar Calculable Resistors (CRs). The research involves Evanohm-S and Isaohm wire heat treatment processes to achieve temperature coefficients less than 0.5 μ Ω / Ω / ∘ C in the CR’s wire resistance element, tests of different terminal−wire joining techniques, and construction aspects achieving a stability of less than 0.05 μ Ω / Ω /day. This kind of construction methodology has not been presented in detail in previous CR papers, and it is essential to accomplish the correct parameters of a CR. Without it, the development of a CR can take several months or even years. A comparison between CRs developed in this research and a CR from the Federal Institute of Metrology (METAS) in Switzerland was carried out. Measurement results between the 10 k Ω octofilar CR and the METAS 1 k Ω coaxial CR show an agreement better than 0.35 μ Ω / Ω through the audio-frequency range. Therefore, the octofilar CR can be used as an AC resistance reference with traceability to the quantum Hall resistance in DC.
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- 2020
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14. Response of Phenolic Compounds in Lippia graveolens Kunth Irrigated with Aquaculture Wastewater and Steiner Solution
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María Isabel Nieto-Ramírez, Ana Angélica Feregrino-Pérez, Humberto Aguirre Becerra, Benito Parra-Pacheco, Mónica Vanessa Oviedo-Olvera, and Juan Fernando García-Trejo
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Plant Science ,antioxidant capacity ,cultivation conditions ,elicitor ,oregano ,secondary metabolites - Abstract
Lippia graveolens is one of the most important aromatic species in Mexico due to antioxidant and antibiotic activities reported in its essential oil. The aim of this work was to assess the effect of irrigation with aquaculture wastewater and salicylic acid addition on the production of phenolic compounds in L. graveolens. L. graveolens plants (14) were irrigated with aquaculture wastewater and (14) using Steiner solution for 28 days; at the same time, salicylic acid was exogenously applied at 0.0 (control), 0.5 and 1.0 mM concentrations in both treatments at 5 and 19 experimental days. The total phenolic content was measured by Folin–Ciocalteu, the flavonoid content was determined by the aluminum chloride method, and the antioxidant capacity was measured by DPPH and FRAP assays. The results showed an increase in the total phenolic and flavonoid content in plants irrigated with aquaculture wastewater solution (17.25 ± 2.35 to 38.16 ± 4.47 mg eq GA·g⁻1 W). The antioxidant capacity was higher in plants irrigated with Steiner solution (98.52 mg eq T·g⁻1 W). In conclusion, L. graveolens irrigated with aquaculture wastewater leads to an increase in the total phenolic content and Steiner-solution antioxidant capacity in plants.
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- 2023
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15. Biologically Active Peptides from Buckwheat (Fagopyrum esculentum Moench) Grain
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Humberto Aguirre-Becerra, Byanka Cruz-Moreno, Ana Patricia Arenas-Salazar, Ma. Cristina Vázquez-Hernández, and Ana Angélica Feregrino-Pérez
- Abstract
Buckwheat (Fagopyrum esculentum Moench) is a pseudocereal with a high content of amino acids, such as lysine; its lack of gluten makes it an attractive product with high nutritional potential that could be included in the human diet. It contains several compounds such as phenolics, carotenoids, organic acids, vitamin E and B complex vitamins, and macro and microelements. Its high protein quality and peptide content are relevant in F. esculentum. Additionally, biologically active peptides (BAPs) have been associated with the prevention of some chronic and neurodegenerative diseases. Consequently, the agro-industrial, cosmetic, food and pharmaceutical sectors have shown a strong interest in this pseudocereal due to its wide range of compounds. This chapter is a compilation of scientific contributions related to buckwheat properties, including generalities, nutritional value, bioactive compounds, BAPs, and finally, perspectives and conclusions. 
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- 2023
16. Observer and parameter estimation for IWP: Comparison of a super-twisting algorithm and an adaptive method.
- Author
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Jesus N. Guerrero-Tavares, Humberto Aguirre-Becerra, and Salvador Ortiz-Santos
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- 2014
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17. Impact of Nanomaterials on Chlorophyll Content in Plants
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Luis Páramo, Humberto Aguirre Becerra, José Emilio Ramírez Piña, José Antonio Cervantes Chávez, Ana A. Feregrino-Pérez, and Karen Esquivel
- Published
- 2023
18. Effect of Extended Photoperiod with a Fixed Mixture of Light Wavelengths on Tomato Seedlings
- Author
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Cristina Vazquez-Hernandez, Luis Miguel Contreras-Medina, Humberto Aguirre-Becerra, Ramón G. Guevara-González, Juan Fernando García-Trejo, Ana A. Feregrino-Perez, and Aurora Mariana Alvarado
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photoperiodism ,Horticulture ,Wavelength ,secondary metabolites ,food and beverages ,light wavelength ,lcsh:SB1-1110 ,seedling ,lcsh:Plant culture ,extended photoperiod ,Mathematics ,photomorphogenesis - Abstract
Light is an abiotic factor, and its quality, quantity, and photoperiod can be modulated to work as eustress inductors to regulate plant processes. It is known that red (R), blue (B), far-red (FR), and ultraviolet-A wavelengths can promote photomorphogenesis and secondary metabolite production in plants. Several ratios of R:B and the addition of end of-day FR, separately, have beneficial effects on plant development, whereas adding ultraviolet-A enhances the production of secondary metabolites such as phenols. However, the effects of extended photoperiods with a mixture of these four wavelengths and extra end-of-day FR have not been evaluated for plants of commercial interest. The objective of this study was to determine the effects on tomato seedlings (‘Saladette’, CORDOBA F1) of different overnight photoperiods using a fixed combination of R (625 nm), B (460 nm), FR (720 nm), and ultraviolet-A (410 nm). We expected increases in the production of specialized metabolites and the generation of beneficial changes in the seedling biomass and morphology. Four treatments involving overnight artificial light provided by a light-emitting diode (LED) module were established: TC (control), 0 h; T1, 4 hours; T2, 8 hours; and T3, 12 hours. All treatments were subjected to a 12-hour natural photoperiod and 12 hours of overnight artificial light. The experiment lasted 4 weeks, and plants were sampled every week for physical and phytochemical measurements. In general, seedlings subjected to 4-hour and 8-hour treatments presented better results than those subjected to the control and 12-hour overnight photoperiod treatments. Seedlings subjected to treatments with an 8-hour overnight photoperiod presented large accumulation of biomass in the stem rather than in the leaves because they had large stem dry weight, stem weight, and elongation and higher first, second, and third internode lengths; however, they had lower leaf area, leaf dry weight, and health index. Seedlings subjected to treatment with a 4-hour overnight photoperiod were visually bigger, with large leaf expansion, total length, stem weight, total weight, and specific leaf area; however, this treatment had a negative impact on the biomass accumulation, with lower leaf weight, stem dry weight, and health index. The 12-hour treatment had a negative impact on the leaf area, and thus the specific leaf area, of seedlings; however, the biomass accumulation was large, with higher leaf dry weight, total dry matter, and specific leaf area, but no difference in stem dry weight compared with the control. At the end of the experiment, the total phenolic content increased in all treatments compared with the control, but the flavonoid content decreased. Moreover, the antioxidant capacity was higher for T2 during the last 2 weeks of the experiment. Results are discussed according to the possibilities of using this light strategy for seedling production.
- Published
- 2020
19. Importance of the Secondary Metabolites and Biological Parameter Modification by Metallic, Oxide, and Carbon-Based Nanomaterials Over Forage Plants
- Author
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Luis Páramo, Ana A. Feregrino-Pérez, Humberto Aguirre Becerra, Ramón G. Guevara-González, and Karen Esquivel
- Published
- 2022
20. Role of Stress and Defense in Plant Secondary Metabolites Production
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Ramón G. Guevara-González, Ma. Cristina Vázquez-Hernández, Aurora Alvarado-Mariana, Humberto Aguirre-Becerra, Ana A. Feregrino-Perez, Juan Fernando García-Trejo, and Diana Saenz de la O
- Subjects
Abiotic component ,Biotic component ,Chemistry ,business.industry ,Mechanism (biology) ,fungi ,Hormesis ,food and beverages ,Biomass ,Biotechnology ,Elicitor ,Plant defense against herbivory ,business ,Secondary metabolism - Abstract
Secondary plant metabolites are natural bioactive compounds which are an important income for the pharmaceutical, food, cosmetic, agriculture, and other sectors due to their health-promoting properties and prevention and treatment of some diseases. The secondary metabolites can be classified into three main groups: phenolic compounds, terpenoids, and nitrogen compounds. The secondary metabolism in plants is a mechanism of adaptation and evolution as a defense to harsh environmental factors that induce stress. According to the hormesis curve of each plant model, the stress can be divided into distress (bad stress that leads to damage and ultimately plant death) or eustress (good stress that leads to activation of secondary metabolism). The environmental factors can be divided into biotic and abiotic which can be artificially induced to activate plant defense responses leading to the production of secondary metabolites. Several approaches to this process called elicitation have been proposed in the last decades with different types of metabolism-inducing factors or elicitors. Novel elicitation using abiotic factors includes electromagnetic waves (including several wavelengths of the light spectra, and electric and magnetic fields), acoustic waves, nanostructures, volatile compounds, nutrient deprivation, and several metals and salt soil pollutants. In the same order, novel elicitation using biotic factors include new bacteria consortium, fungi, phytohormones, and miRNA solutions. In general, the purpose of elicitation is to interact with the biochemical routes in order to produce secondary metabolites in high quantities, usually with negative effects in biomass production or morphology, but increasing plant quality in terms of aroma, taste or color. The metabolic profile and general response of elicitation vary greatly depending on the plant model, the elicitor level or concentration, and the stimulation time. Considering these facts, this chapter clearly and concisely discusses the most current strategies of elicitation for the increase of secondary metabolites production in plants.
- Published
- 2020
21. Jacaranda flower (Jacaranda mimosifolia) as an alternative for antioxidant and antimicrobial use
- Author
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Silvia Araceli Pineda-Nieto, Beatriz Liliana Álvarez-Mayorga, Humberto Aguirre-Becerra, Ramón G. Guevara-González, Juan Fernando García-Trejo, Dulce María Rivera Pastrana, and Ana A. Feregrino-Perez
- Subjects
0301 basic medicine ,DPPH ,03 medical and health sciences ,chemistry.chemical_compound ,Rutin ,0302 clinical medicine ,Food science ,Agar diffusion test ,Gallic acid ,lcsh:Social sciences (General) ,lcsh:Science (General) ,Multidisciplinary ,ABTS ,Jacaranda mimosifolia ,biology ,Chemistry ,biology.organism_classification ,Antimicrobial ,Phenolic compounds ,030104 developmental biology ,Polyphenol ,lcsh:H1-99 ,Antioxidant ,030217 neurology & neurosurgery ,lcsh:Q1-390 ,Research Article - Abstract
Antimicrobial resistance to antibiotics is a serious health problem worldwide, for this reason, the search for natural agents with antimicrobial power against pathogenic microorganisms is of current importance. The objective of this work was to evaluate the antioxidant capacity (ABTS+ and DPPH), antimicrobial activity, and polyphenol compounds of methanolic and aqueous extracts of Jacaranda mimosifolia flowers. The antimicrobial activity against Bacillus cereus ATCC 10876, Bacillus subtilis ATCC 6633, Enterococcus faecalis ATCC 51299, Escherichia coli ATCC 25922, Listeria monocytogenes ATCC 19115, Pseudomonas aeruginosa ATCC 27853, Salmonella typhimurium ATCC 14028, Staphylococcus aureus ATCC 25923, and Streptococcus mutans ATCC 25175, was determined using the Kirby Bauer technique. The results of polyphenolic compounds showed a high amount of total flavonoids in the methanolic and aqueous extracts (503.3 ± 86.5 and 245. 7 ± 27.8 mg Rutin Equivalents/g DW, respectively). Quercetin, gallic acid, caffeic acid, and rutin were identified by the HPLC-DAD technique, while in the GC-MS analysis, esters, fatty acids, organic compounds, as well as monosaccharides were identified. Higher antioxidant capacity was detected by the ABTS technique (94.9% and 62.6%) compared to DPPH values (52.5% and 52.7 %) for methanolic and aqueous extracts, respectively. The methanolic extract showed a greater inhibitory effect on gram-positive bacteria, with a predominant higher inhibition percentage on Listeria monocytogenes and Streptococcus mutans (86% for both). In conclusion, Jacaranda flower extracts could be a natural antimicrobial and antioxidant alternative due to the considerable amount of polyphenolic compounds, and serve as a sustainable alternative for the isolation of active ingredients that could help in agriculture, aquaculture, livestock, pharmaceutics, and other industrial sectors, to remediate problems such as oxidative stress and antimicrobial abuse., Jacaranda mimosifolia; Antioxidant; Antimicrobial; Phenolic compounds.
- Published
- 2020
22. A Simple Methodology to Develop Bifilar, Quadrifilar, and Octofilar Calculable Resistors
- Author
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Juvenal Rodríguez-Reséndiz, Alepth H. Pacheco-Estrada, Felipe L. Hernandez-Marquez, Carlos Duarte-Galvan, Luis Miguel Contreras-Medina, Humberto Aguirre-Becerra, and Carlos D. Aviles
- Subjects
Bifilar coil ,02 engineering and technology ,010501 environmental sciences ,Quantum Hall effect ,calculable resistor ,lcsh:Technology ,01 natural sciences ,law.invention ,lcsh:Chemistry ,law ,Simple (abstract algebra) ,General Materials Science ,lcsh:QH301-705.5 ,Instrumentation ,Wire resistance ,reference standard ,0105 earth and related environmental sciences ,Fluid Flow and Transfer Processes ,Physics ,evanohm heat-treatment ,lcsh:T ,Process Chemistry and Technology ,General Engineering ,021001 nanoscience & nanotechnology ,lcsh:QC1-999 ,Computer Science Applications ,Computational physics ,Metrology ,lcsh:Biology (General) ,lcsh:QD1-999 ,lcsh:TA1-2040 ,frequency dependence ,impedance metrology ,Ac resistance ,Resistor ,Coaxial ,lcsh:Engineering (General). Civil engineering (General) ,0210 nano-technology ,lcsh:Physics - Abstract
This paper describes the development of bifilar, quadrifilar, and octofilar Calculable Resistors (CRs). The research involves Evanohm-S and Isaohm wire heat treatment processes to achieve temperature coefficients less than 0.5 &mu, &Omega, / &Omega, / ∘ C in the CR&rsquo, s wire resistance element, tests of different terminal&ndash, wire joining techniques, and construction aspects achieving a stability of less than 0.05 &mu, /day. This kind of construction methodology has not been presented in detail in previous CR papers, and it is essential to accomplish the correct parameters of a CR. Without it, the development of a CR can take several months or even years. A comparison between CRs developed in this research and a CR from the Federal Institute of Metrology (METAS) in Switzerland was carried out. Measurement results between the 10 k &Omega, octofilar CR and the METAS 1 k &Omega, coaxial CR show an agreement better than 0.35 &mu, through the audio-frequency range. Therefore, the octofilar CR can be used as an AC resistance reference with traceability to the quantum Hall resistance in DC.
- Published
- 2020
- Full Text
- View/download PDF
23. Effect of Two Levels of Temperature and Natural and Artificial Photoperiod on Growth and Metamorphosis of Bullfrog Tadpoles in an Intensive Rearing System
- Author
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Guillermo Abraham Peña-Herrejón, Humberto Aguirre-Becerra, Andre Muniz‐Afonso, Ana A. Feregrino-Perez, Genaro M. Soto-Zarazúa, and Juan Fernando García-Trejo
- Subjects
0106 biological sciences ,0301 basic medicine ,photoperiodism ,media_common.quotation_subject ,Zoology ,Aquatic Science ,Biology ,010603 evolutionary biology ,01 natural sciences ,03 medical and health sciences ,030104 developmental biology ,Bullfrog ,Metamorphosis ,media_common - Published
- 2018
24. Influence of Elicitors and Eustressors on the Production of Plant Secondary Metabolites
- Author
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Ramón G. Guevara-González, Juan Fernando García-Trejo, Aurora Mariana Alvarado, Ixchel Parola-Contreras, Ma. Cristina Vázquez-Hernández, Ana A. Feregrino-Perez, Laura Helena Caicedo-Lopez, Luis Miguel Contreras-Medina, Humberto Aguirre-Becerra, and Ernesto Magaña-Lopez
- Subjects
Abiotic component ,chemistry.chemical_classification ,fungi ,food and beverages ,Primary metabolite ,Metabolism ,Biology ,Plant cell ,Tissue culture ,Biochemistry ,chemistry ,Tissue cell ,Time of use ,Carotenoid - Abstract
Plants are the main source of secondary metabolites, which can be used in different sectors such as pharmaceutics, food, cosmetics, agriculture, etc. turning them into an attractive source of income. Primary metabolites (carbohydrates, lipids, and proteins) have been linked to vital processes such as growth, development, and fruiting, while the secondary metabolites (phenols, flavonoids, and carotenoids, to name a few) are the result of adaptation and evolution of the plants with respect to changes in the environment. Therefore, it can be said that the secondary metabolites are secreted when the plant is under biotic and abiotic stresses. These secondary metabolites possess several bio-active functions and, hence, are well recognized for industrial applications. Due to difficulties for extracting these natural plant bio-active compounds, they are usually produced alternatively through plant cell/tissue culture methods. Further, several approaches, such as the use of plant and tissue cell cultures, the application of metabolism-inducing factors or elicitation, control of biological factors, such as intensity of light, induction by sound waves, and application of nanoparticles are used to alter/enhance in vitro production of these bio-active metabolites. Overall, these approaches interact with the biochemical routes of the plant either in positive or negative ways to produce secondary metabolites in high quantities. Although there is information on this aspect, the effect of these strategies cannot be generalized, since it has been observed that the metabolism of the plant response depends on the study model, the concentration and time of use of the stimulus, as well as its nature. Considering the above facts, this chapter discusses on the most current strategies for the production of secondary metabolites in plants in a continuous and reliable manner.
- Published
- 2019
25. Growth and Metabolic Effects of Stocking Density in Bullfrog Tadpoles (Rana catesbeiana) under Greenhouse Conditions
- Author
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Perla V. Munguia-Fragozo, Oscar Alatorre-Jacome, Humberto Aguirre-Becerra, Juan F. García-Trejo, Genaro M. Soto-Zarazúa, and Enrique Rico-García
- Subjects
General Agricultural and Biological Sciences - Published
- 2015
26. Breeding system for Astyanax mexicanus
- Author
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Julieta Sanchez-Velazquez, Guillermo Abraham Peña-Herrejón, Fernando Garcia-Trejo, Humberto Aguirre-Becerra, and Andrez Cruz-Hernandez
- Subjects
Fishery ,geography ,geography.geographical_feature_category ,Cave ,Aquaculture ,Ecology ,business.industry ,%22">Fish ,Biology ,business - Abstract
Astyanax mexicanus is a Mexican native fish that have surface and cave phenotypes. Due to their distinctive characteristics caused by and independent evolution it is a model for studies on evolution and development, and it could be used for cross-species comparisons in aquaculture since it is close related to economically important species. A. mexicanus have been in the aquarium trade in the United States since the early 1940s, however, there are no reports of its production inside Mexico. We propose a breeding system to enable the establishment of experimental and commercial lineages available for trade. The system was made in a rack structure, with interchangeable modular fish tanks. To induce the spawning of the species, an overnight temperature change its needed, requiring an increment for the cave fish and a temperature reduction for the surface fish. A temperature regulation trial was performed, changing the temperature from 26°C to 22°C overnight and turning it back to 26°C at the next morning. The obtained temperature gradient was adequate to give the spawning condition of the species as stated in previous studies. The proposed system its adequate to start breeding trials with different A. mexicanus populations.
- Published
- 2017
27. LED grow light for Lemna gibba plant
- Author
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Morales, Ortiz, primary, Gibran, Ismael, additional, Humberto, Aguirre Becerra, additional, Trejo Juan Fernando, Garcia, additional, Putzu-Torres, Anna Paola, additional, and Jacome Oscar, Alatorre, additional
- Published
- 2018
- Full Text
- View/download PDF
28. Thermal feasibility of tilapia production under greenhouse conditions in a semi-desert region of Mexico
- Author
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Julieta Sanchez-Velazquez, Guillermo Abraham Peña-Herrejón, Fernando Garcia–Trejo, and Humberto Aguirre-Becerra
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food.ingredient ,biology ,business.industry ,Environmental engineering ,Greenhouse ,Tilapia ,biology.organism_classification ,Nile tilapia ,Animal science ,food ,Aquaculture ,Productivity (ecology) ,Fish growth ,Environmental science ,%22">Fish ,Semi desert ,business - Abstract
Nile tilapia do not achieve the desired productivity in the semi-desert regions of Mexico, because of their temperature variations. Temperature needs to be regulated to obtain commercial weights in 6 months, as stated by governmental agencies. With the use of greenhouses it is not possible to maintain all year round the optimum water for tilapia production, thus the temperature effect needs to be considered. A comparison was performed between two different semi-arid environmental temperatures. Fingerlings were stocked (60 days) by triplicate inside a greenhouse. Water, greenhouse and environmental temperatures were measured. Fish were weighed at the end. The weekly average values were compared graphically. External temperatures were of 20.5 ± 6.7 (ET=20.5) and 16.9 ± 8.4 °C (ET=17) respectively. ET=20.5 °C showed a more stable water (26.8 ± 1.3 °C) and greenhouse temperatures (24.4 ± 4.4 °C) while ET=17 °C had a greater variation of water (22 ± 3.3 °C) and greenhouse (18.7 ± 5.5 °C) temperatures. Tilapia under ET=20.5 °C achieved a better weight of 22.23 ± 1.85 g whit a tendency for bigger weights while Tilapia under ET=17 °C achieved a more uneven weight of 16.21 ± 3.30 with a tendency for smaller fish. The temperature and growth variation will have to be considered when planning a tilapia production project to obtain the desired weights. Tilapia under greenhouse in semi-arid regions of Mexico, without any other technology, will only be feasible, according to the plans proposed by the government, during the warmer period of the year.
- Published
- 2016
29. Diseño e implementación de un sistema de incubación de renacuajos de rana toro (Rana catesbeiana, Shaw1802) basados en el control e intensidad luminosa y temperatura
- Author
-
HUMBERTO AGUIRRE BECERRA
- Subjects
11 [cti] ,7 [cti] - Abstract
La acuicultura permite producir animales y plantas acuáticas para consumo humano de forma intensiva a través del control de variables físicas y químicas del agua donde se desarrolla el cultivo. El cultivo de rana toro forma parte importante de la acuicultura. En México se establecieron granjas de cultivo de rana toro, adaptando sistemas de producción (diseño de granja, sistemas de control, alimentación, etc.) de países como Brasil y China sin éxito debido a las diferentes condiciones climáticas. La fase de renacuajo es crítica en el cultivo de este animal, ya que los organismos que sobrevivan esta etapa metamorfizarán y se convertirán en rana joven, la cual será la base para la engorda y futura venta. La etapa larval de la rana presenta un bajo nivel de sobrevivencia y bajos niveles de crecimiento. Este trabajo presenta un sistema de control de temperatura y fotoperiodo artificialcon la finalidad de obtener mayores parámetros de crecimiento e índice de supervivencia en el renacuajo. La temperatura se controla recirculando agua caliente a través de un intercambiador de calor instalado en el estanque. El fotoperiodo artificial es controlado a través de una lámpara de 90 leds de luz blanca de alta intensidad. El controlador cuenta con una interfaz de usuario basada en una pantalla táctil capacitiva. El sistema cuenta con un datalogger a través de una memoria uSD que el usuario retira de la pantalla táctil, inserta en su computadora y abre el archivo de texto generado por la pantalla. Se hicieron pruebas de validación para el funcionamiento, y se realizó una prueba para evaluar el efecto del sistema sobre los renacuajos. Los tratamientos que presentaron mejores parámetros de crecimiento fueron los que contaban con control de temperatura. El fotoperiodo artificial influyó negativamente en el crecimiento de los renacuajos. Sin embargo, el tratamiento que presentó mayor nivel de supervivencia fue el de fotoperiodo. Los tratamientos con control de fotoperiodo muestran mayores niveles de nitritos y nitratos en el agua. Los tratamientos con control de temperatura muestran menores niveles de amonio en el agua. Aquaculture allows the animal and plants intensive production for human consumption through the control of physical and chemical variables of the water where the animal develops. Bullfrog farming is an important part of aquaculture. Farms were established in Mexico for bullfrog culture, adapting production systems (farm design, control systems, food, etc.) of countries like Brazil and China without success due to different weather conditions. The tadpole stage is critical, since the organisms that survive this stage will metamorphose and will become young frog, which will be the basis for further fattening and sale. The Larval stage of the frog has low survival and growth parameters. This paper presents a temperature and artificial photoperiod control system in order to obtain greater growth and survival parameters in a tadpole culture. Temperature is controlled by recirculation of hot water through a heat exchanger installed in the pond. Artificial photoperiod is controlled through a 90 high intensity white light LED lamp. The controller has a user interface based on a capacitive touch screen. The system has a datalogger through uSD memory that the user takes out from the touch screen, inserts it in a computer and opens the text file generated by the touchscreen. Validation tests were performed for operation behavior, and testing was performed to evaluate the effect of the system on the tadpoles. Treatments with temperature control showed better growth parameters. Artificial photoperiod negatively influenced the tadpole growth. However, the treatment which showed higher survival level was artificial photoperiod. Photoperiod control treatments show higher levels of nitrites and nitrates in the water. Temperature control treatments show lower ammonia levels in the water
- Published
- 2014
30. Panorama general y programas de protección de seguridad alimentaria en México
- Author
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Humberto Aguirre Becerra, Juan Fernando García Trejo, María Cristina Vázquez Hernández, Aurora Mariana Alvarado, and Hilda Romero Zepeda
- Subjects
food security ,Mexico ,nutrition ,Medicine - Abstract
Proporcionar seguridad alimentaria a la población representa un desafío global, especialmente para los países en vías de desarrollo debido a su insostenible crecimiento poblacional, bajo nivel de ingresos y tasa acelerada de urbanización. En México, un alto porcentaje de la población no tiene acceso a una canasta básica y por ende posee algún grado de inseguridad nutricional. Existen programas gubernamentales que han procurado mitigar este problema al proveer una cantidad suficiente de alimentos, sobretodo en poblaciones vulnerables como comunidades indígenas, rurales, niños y mujeres. Por otra parte, los alarmantes índices de obesidad y sobrepeso que han elevado la morbilidad de enfermedades crónicas como hipertensión y diabetes en la población, reflejan un problema relacionado con el hambre oculta, aquella donde hay una deficiencia de micronutrientes al enfocarse en una dieta alta en contenido energético, evidenciando la falta de calidad nutritiva en los alimentos y desinformación en la población mexicana. El presente artículo muestra la situación de seguridad alimentaria en México y algunos programas gubernamentales que procuran mitigar este problema, de los cuales solo algunos prestan poca atención a los aspectos nutricionales, restringiendo el derecho a una alimentación de calidad.
31. Consumo actual de edulcorantes naturales (beneficios y problemática): Stevia
- Author
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María Cristina Vázquez Hernández, Ramón Gerardo Guevara González, Humberto Aguirre Becerra, Aurora Mariana Alvarado, and Hilda Romero Zepeda
- Subjects
additive ,diterpenoid steviol glycosides ,Stevia ,sweetener ,Medicine - Abstract
La estevia [Stevia rebaudiana (Bertoni)] es un arbusto de tipo perenne de la familia de las Asteraceas que crece en áreas tropicales y subtropicales de Suramérica. Hoy en día, su cultivo se ha extendido a otras regiones del mundo, incluyendo Canadá y algunas partes de Asia, Europa y México, donde sus hojas se han utilizado tradicionalmente como edulcorante natural durante cientos de años. En la actualidad, el uso potencial y las implicaciones prácticas de la estevia como un edulcorante se muestran en una serie de alimentos procesados, ya que contiene glucósidos de esteviol como ingrediente activo, que puede ser bajo o no calórico, y hasta 100-300 veces más dulce que la sacarosa. Además, las hojas secas de estevia contienen también minerales, vitaminas, compuestos fenólicos, flavonoides y otros compuestos antioxidantes, con propiedades antimicrobianas y antioxidantes. Stevia acumula hasta un 30 % de los glucósidos de esteviol (SGs por su sigla en inglés) del peso seco de las hojas. El esteviósido y el rebaudiósido A son las principales SGs. Desde diciembre de 2011, los SGs (E 960) se han autorizado como aditivo alimentario y edulcorante en Estados Unidos. Su uso en diversas categorías de alimentos está regulado como por ejemplo en suplementos alimenticios y alimentos dietéticos para propósitos médicos especiales y control de peso. Sin embargo, la información ofrecida al consumidor es engañosa y dista de ser confiable. Este artículo ofrece al público interesado, datos que deben de ser evaluados al comprar productos adicionados con estevia.
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