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In view of the strong acidity and high heavy metal contents of the soil, the low vegetation cover, and strong soil erosion caused by mining activities, the reasonable determination of the cubic restoration mode is the key to determining the good or bad ecological restoration effects on mining wasteland. In this study, based on field experiments, a combined cubic ecological restoration scheme for soil improvement-vegetation reconstruction was constructed. Using analysis of variance, a regression model, and the Mantel test, the differences in soil properties and the biodiversity were analyzed under different restoration schemes, the entropy-weighted-TOPSIS method was used to optimize the best ecological restoration model. The results revealed that compared with the pre-restoration state, the restoration significantly increased the soil pH (p0.05) by 4.07-5.73, regulated the strong acidic environment of the soil, increased the organic matter content by 5.35-11.21 times, and improved the soil fertility. The available contents of Pb and Cd were reduced by 67.15-75.58 % and 64.15-88.68 %, respectively compared with the background values. Biodiversity improved significantly, and the available content of Cd was an important factor in the biodiversity recovery. The evaluation of the effect of the restoration scheme showed that the combination of mixed soil amendments of rice husks and chicken manure (10 kg/m

A defect pyrochlore-type Sn1.06Nb2O5.59F0.97 (SnNbOF) nano-octahedron is used as a redox-active support for fabricating Au@SnO2 core-shell and SnO2 quantum dots at room temperature without the use of organic species or foreign reducing reagents. Gold (Au) and SnO2 components were obtained through an in situ redox reaction between the HAuCl4 and reductive Sn2+ ions incorporated in SnNbOF. The composition and morphology of the resulting nanocomposites (denoted as Au-SnNbOF) could be controlled by adjusting the Au/SnNbOF ratio. The Au-SnNbOF nanocomposites exhibited efficient photoactivities for methyl orange (MO) degradation under the visible light irradiation (λ > 420 nm), during which the MO was almost completely degraded within 8 min. Among all the samples, the 5wt% Au-SnNbOF nanocomposite had the highest rate constant (0.43 min-1), which was 40 times higher than that of the blank SnNbOF. ispartof: RSC ADVANCES vol:10 issue:56 pages:33955-33961 ispartof: location:England status: published

Background: Most studies have shown that Beta-Casomorphin-7 (BCM-7) has many physiological functions, this study aims to investigate the effects of BCM-7 on renal function and immunity in naturally aging mice and to reveal the effects of BCM-7 on health and provide more theoretical reference for inflammatory aging.Methods: All mice were weighed weekly and the organ indexes were calculated, Paraffin sections were prepared for kidney histopathological examination with conventional Haematoxylin and Eosin (HE) staining, renal function indexes and cytokines were main measured by ELISA and biochemical colorimetry.Result: The weight of each group increased during gastric perfusion and the higher the intervention concentration, the lower the weight gain rate. The renal index was not affected by BCM-7, but the spleen index was significantly decreased by medium-dose BCM-7. There were inflammatory cell infiltration and other renal lesions in the aging control group and intervention group. A medium dose of BCM-7 can significantly reduce Cystatin C (Cys-C) content in aging mice, the content of Adrenocorticotropic hormone (ACTH) in aged mice decreased with the increase of BCM-7 intervention dose. BCM-7 and aging caused cytokine imbalance in mice.

Scope The aim of this study is to examine whether lycium barbarum polysaccharide (LBP) supplementation improves hyperglycemia and inflammation in diabetic KKAy mice. Methods The successfully established diabetic KKAy mice are randomized into five groups: diabetic model, metformin, low‐dose LBP, middle‐dose LBP, and high‐dose LBP . C57BL/6J mice are fed a chow diet as normal control . The blood glucose and body weight of mice were measured at different time points. At the end of 90 days, serum inflammatory factors were determined with ELISA kits. The expression of TLR4, MyD88, TRAF6, IκKβ, IκB, P-IκB and nuclear NF-κB proteins in mouse peritoneal macrophages were detected by Western Blotting. Results Blood glucose decreased significantly after the intervention among low-, medium-dose LBP groups and Met group ( P

Scope: RAW264.7 cells was used to study the regulatory effect of LBP on I κ B phosphorylation and NF - κ B nuclear translocation.Methods: (1)After 5, 10, 20 and 30 minutes of LBP intervention, Western blotting was used to detect the expression of I κ B and P-I κ B in each group, (2)After 5 minutes of intervention with different doses of LBP,Western blotting was used to detect the expression of P-I κ B and I κ B in the cells of each group, (3)After 30 minutes of LBP intervention, the distribution of NF - κ B nuclear translocation was observed by immunofluorescence, and the number of NF - κ B nuclear translocation was detected by Western blotting.Results : The results of the time-dependent regulation of I κ B phosphorylation by LBP showed that when stimulated by LPS, I κ B phosphorylated rapidly, the level of P-I κ B increased and I κ B decreased, and the level of I κ B increased at each time point in the combined treatment group of LPS (1 μ g / ml) + LBP (25 μ g / ml).After LPS stimulation, the expression of I κ B decreased and the expression of phosphorylated I κ B increased (P < 0.05); after LPS (L μ g / ml) + LBP (25,50 μ g / ml), the level of I κ B increased (P < 0.05).After LPS stimulation, the green fluorescence was mainly distributed in the nuclear area, and after LBP stimulation, the distribution of green fluorescence was slightly increased; in LPS + LBP group, the distribution of green fluorescence in the cytoplasm was increased, and the distribution of green fluorescence in the nucleus was decreased. After LPS stimulation, NF - κ B level in cytoplasm decreased (P < 0.05), and NF - κ B level in nucleus increased significantly (P < 0.05). After LPS (L μ g / ml) + LBP (25, 50, 100 μ g / ml), NF - κ B level in cytoplasm increased (P < 0.05), and NF - κ B level in nucleus decreased (P < 0.05).Conclusion: Collectively, these results suggested that LBP's anti-inflammatory effects were relevant to the interruption of NF-kappa B activation.

Abdominal obesity is associated with an increased risk of insulin resistance, which may be a potential contributor to dyslipidemia. However, the relationship between postprandial insulin resistance and lipid metabolism in abdominally obese subjects remains unknown. We hypothesized that postprandial dyslipidemia would be exaggerated in abdominally obese subjects with high postprandial insulin resistance. To test this hypothesis, serum glucose, insulin, triglycerides, total cholesterol, high-density lipoprotein cholesterol, and apolipoprotein B were measured at baseline and postprandial state at 0.5, 1, 2, 4, 6, and 8 hours after a liquid high-fat meal in non-abdominally obese controls (n=44) and abdominally obese subjects with low (AO-LPIR, n=40), middle (n=40), and high postprandial insulin resistance (AO-HPIR, n=40) based on the tertiles ratio of the insulin to glucose areas under the curve (AUC). Their serum adipokines were tested at baseline only. Fasting serum leptin was higher (P

To investigate the effect of: Diabetes was induced by feeding high-fat/high-sugar diet and injection of low-dose streptozotocin in MyD88-knockout mice. The diabetic mice were randomly divided into model group, positive control group and LBPs group. The expressions of TRAM, TRIF, TRAF6, RIP1 and TNF-α mRNA and proteins in mouse peritoneal macrophages were detected by real-time RT-PCR and Western blotting after LBPs treatment for 3 month. Serum TNF-α was determined with ELISA kit.: Real time RT-PCR showed that compared with model group, the relative expressions of: LBPs may not inhibit serum TNF-α level through TLR/NF-κB independent pathway.

Lycium barbarum polysaccharide (LBP) has traditionally been used in Chinese medicine as a chief ingredient of Lycium barbarum (wolf berry/goji berry) for the treatment of various diseases with the symptoms of frequent drinking and urination. This study was conducted as a randomized, controlled clinical trial. A total of 67 patients with type 2 diabetes (30 in control group and 37 in LBP group) were enrolled in this prospective, randomized, double-blind study (administration at 300mg/day body weight). In order to observe the hypoglycemic and lipid-lowering activity of LBP in patients with type 2 diabetes after dinner, various tests were conducted between control and LBP intervention groups in 3 months. Although, the study had small sample size and short follow-up, significant findings were observed. The results of our study indicated a remarkable protective effect of LBP in patients with type 2 diabetes. Serum glucose was found to be significantly decreased and insulinogenic index increased during OMTT after 3 months administration of LBP. LBP also increased HDL levels in patients with type 2 diabetes. It showed more obvious hypoglycemic efficacy for those people who did not take any hypoglycemic medicine compared to patients taking hypoglycemic medicines. This study showed LBP to be a good potential treatment aided-agent for type 2 diabetes.

The effects of Lycium barbarum L. (L. barbarum) on the cardiometabolic risk factors from randomized controlled trials (RCTs) have shown inconsistent results. The present meta-analysis aimed to investigate the effects of L. barbarum supplementation on the cardiometabolic risk factors. A systematic literature search was performed in Chinese National Knowledge Infrastructure (CNKI), PubMed, Scopus, and Wanfang databases updated to March 2017. The mean changes in cardiometabolic risk factors were calculated as the weighted mean difference (WMD) using a random-effects model. Seven RCTs with a total of 548 subjects were included. The pooled estimate showed that L. barbarum intervention significantly reduced the fasting glucose concentrations (−0.36 mmol L−1/−6.5 mg dL−1; 95% confident interval (CI): −0.62, −0.10 mmol L−1/−11.3, −1.8 mg dL−1). In addition, L. barbarum supplementation marginally reduced the concentrations of total cholesterol (TC) (−0.30 mmol L−1/−11.6 mg dL−1; 95% CI: −0.75, 0.15 mmol L−1/−29.0, 5.8 mg dL−1; P = 0.189) and triglyceride (TG) (−0.20 mmol L−1/−17.7 mg dL−1; 95% CI: −0.46, 0.05 mmol L−1/−40.7, 4.4 mg dL−1; P = 0.122), but the summary estimates did not reach statistical significance. No benefit was found in relation to bodyweight and blood pressure. The present meta-analysis provides some evidence that supplemental L. barbarum might have favourable effect on glucose control.

MC4R (melanocortin-4 receptor) gene polymorphisms have been associated with serum triglycerides (TG) in Caucasians and Japanese, but no reports are available Chinese. The purpose of this study was to find whether there was an association of rs17782313 polymorphisms at the MC4R gene with serum TG in elderly Chinese.2,012 over 40 years participated in a cross-sectional study in which their body mass index (BMI), TG, high density lipoprotein-cholesterol (HDL-C), and MC4R rs17782313 polymorphisms were determined.For women, carriers of the T/T genotype had significantly lower serum TG than those with C/C genotype (p=0.006). Carriers of the C/C genotype of this polymorphisms exhibited significantly lower fasting HDL-C levels compared with T/T and T/C genotypes (p=0.025), and increased glycosylated hemoglobin (HbA1c) (p=0.043), but no change in blood pressure. Higher serum TG in carriers of the C/C genotype of MC4R gene remained stable after adjustment for age, smoking, drinking, BMI, waist circumference (WC) and three or more components of the metabolic syndrome (MS) by multivariable linear regression (p=0.01) in women. The carriers of the C/C genotype of MC4R gene showed significantly greater odds ratio for TG than T/C and T/T genotypes, even when adjusted for age, smoking, drinking, BMI and WC in women.The rs17782313 C/C genotype is associated with higher TG levels in older Chinese women.背景与目的：研究显示高加索人和日本人的黑色素皮质激素-4受体（MC4R） 基因多态性与血清甘油三酯（TG）相关，但没有关于中国人的报道。本研究 的目的是研究黑色素皮质激素-4受体基因rs17782313多态性是否与中国老年人 血清甘油三酯有关。方法与研究设计：本研究采用横断面的研究方法调查了 2012位年龄在40岁以上的成年人，测定其体质指数、甘油三酯、高密度脂蛋白 胆固醇以及黑色素皮质激素-4受体基因rs17782313多态性等指标。结果：携带 T/T基因型的女性血清甘油三酯明显低于携带C/C基因型的女性（p=0.006）。 携带C/C基因型的女性的高密度脂蛋白胆固醇则明显低于携带T/T和T/C基因型 的女性（p=0.025），但其糖化血红蛋白显著高于携带T/T和T/C基因型的女性 （p=0.043），血压无统计学意义。在校正了年龄、吸烟、饮酒、体质指数、 腰围和代谢综合征等因素后，多元线性回归分析发现携带C/C基因型的女性有 较高的血清甘油三酯（p=0.01）。在女性中，即使校正了年龄、吸烟、饮酒、 体质指数和腰围，携带C/C基因型的女性甘油三酯的比值比（OR值）仍然显 著高于携带T/C和T/T基因型的女性。结论：中国老年女性的黑色素皮质激素-4 受体基因rs17782313的C/C基因型与较高的血清甘油三酯有关。.