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A highly sensitive lateral flow immunoassay (LFIA) is developed for the enzyme-catalyzed and doublereading determination of clenbuterol (CLE), in which a new type of probe was adopted through the direct electrostatic adsorption of ultra-small copper-gold bimetallic enzyme mimics (USCGs) and monoclonal antibodies. In the assay, based on the peroxidase activity of USCG, the chromogenic substrate TMB-H2O2 was introduced to trigger its color development, and the results were compared with those before catalysis. The detection sensitivity after catalysis is 0.03 ng mL-1 under optimal circumstances, which is 6-fold better than that of the traditional Au NPs-based LFIA and 2-fold greater than that before catalysis. This approach was successfully applied to the detection of CLE in milk, pork and mutton samples with an optimum assay time of 7 min and best catalytic time of 80 s, after which satisfactory recoveries of 98.53-117.79% were obtained. Cu-Au nanoparticles as a signal tag and the use of their nanozyme properties are the first applications in the field of LFIA. This work can be a promising exhibition for the application of a cheaper substitute for HRP, ultra-small bimetallic enzyme mimics, in LFIAs. [ABSTRACT FROM AUTHOR]

Solar thermal and photovoltaic unit on traditional PV/T system are restrict each other because the collector is installed on the photovoltaic unit. Some coating cover plates were prepared with thermochromic materials of that application in industry, textiles and daily life. The coating cover plate will adjust its optical properties due to the temperature. An innovative thermochromic coating PV/T system was established. The comprehensive experimental device was set up for carrying out test in simulation. Comparing with the fluid beam-splitting PV/T system, there is more difference in the ray transmittance of the thermochromic coating cover plate before periods after discoloration. When temperature is less 45 °C, the heat-collecting effect is better whereas more 45 °C it decreases and then the ray transmittance and the performance of photovoltaic power generation for system are improved. When the mass fraction of 500 nm graphite powder added to the thermochromic coating is 2‰, the heat-collecting effect is the best, and the rate of primary energy utilization of the system is 40.1% in maximum value. The thermochromic coating PV/T system can flexibly adjust the use of photovoltaic power and thermal energy. When the water temperature is lower, the thermochromic coating PV/T system mainly collects heat and generates electricity as a supplement. After reaching the hot water requirement, the system reduces heat-collecting function and improves the photovoltaic power generation capacity, and it is of great practical value. The research results can provide a new solutions method for the application of PV/T systems.

This paper presents an input–output linearization (IOL) control strategy to achieve PFC (power factor correction) and output voltage constant in a boost converter. The method transforms the nonlinear system to a linear one in new coordinates by the appropriate nonlinear coordinate transformation and state feedback, ultimately allowing the controller to be designed according to the classical linear control theory. An example implementation is simulated using PSIM software. The simulation results show that the phases of input voltage and input current are consistent, and the output voltage remains constant under load step changes. A set of experimental devices are set up to verify the control method, and the results are consistent with the simulation. Thus, the method is proved to be effective in achieving PFC and provides robust constant voltage output in the boost converter.

The accuracy of localization and mapping of automated guided vehicles (AGVs) using visual simultaneous localization and mapping (SLAM) is significantly reduced in a dynamic environment compared to a static environment due to incorrect data association caused by dynamic objects. To solve this problem, a robust stereo SLAM algorithm based on dynamic region rejection is proposed. The algorithm first detects dynamic feature points from the fundamental matrix of consecutive frames and then divides the current frame into superpixels and labels its boundaries with disparity. Finally, dynamic regions are obtained from dynamic feature points and superpixel boundaries types; only the static area is used to estimate the pose to improve the localization accuracy and robustness of the algorithm. Experiments show that the proposed algorithm outperforms ORB-SLAM2 in the KITTI dataset, and the absolute trajectory error in the actual dynamic environment can be reduced by 84% compared with the conventional ORB-SLAM2, which can effectively improve the localization and mapping accuracy of AGVs in dynamic environments.

The studies on the paleo-fluid in the Ordovician–Silurian detachment zone in the Middle–Upper Yangtze area focus on the origin of high-density methane inclusions and the evolution process of formation pressure, but rarely deal with the significance of paleo-fluid to shale gas preservation. In this paper, the relationship between fracture formation and detachment zone was analyzed by observing the Ordovician–Silurian outcrops in western Hunan–Hubei area and by investigating the geological characteristics of fracture veins in the drilling cores. Then, the significance of paleo-fluid forming environment and detachment zone to shale gas preservation was studied by using inclusion compositions of fracture veins and homogenization temperature test data. Finally, accumulation–dispersion modes of shale gas in the detachment zone were established. The following results were obtained. First, the detachment zone is lithologically composed of silicite with intercalated shale at the Ordovician–Silurian interface. In the detachment zone, rocks are broken and small crumples are developed. The conjugate-vertical joints are relatively developed in the silicite above and below the detachment zone. Second, multi-stage and multi-type inclusions, especially the aqueous inclusions, are developed in the veins of the detachment zone. Third, the infiltration depth of ancient meteoric water along the detachment zone in the study area is over 4000 m. High-density overpressure methane inclusions were captured in the detachment zone during early stage, while normal-pressure methane and nitrogen inclusions were captured during late stage. Fourth, the ionic constituents of inclusions recording the fluid activity during the late stage was characterized by high sodium-chloride coefficient, high desulfurization coefficient and low metamorphic coefficient, and it is indicated that the sealing capacity of the shale in the detachment zone gets worse. Fifth, the accumulation and dispersion of shale gas in the detachment zone within the study area is divided into three modes, i.e., syncline, broad anticline and closed anticline. In conclusion, the detachment zone in the study area is permeable. Synclines and closed anticlines developed in detachment structures are unfavorable for the accumulation of shale gas, while broad anticlines are favorable. Keywords: Western Hunan–Hubei area, Ordovician, Silurian, Detachment zone, Paleo-fluid, Inclusions, Homogenization temperature, Ionic constituent, Shale gas, Preservation condition

The traditional simultaneous localization and mapping (SLAM) system uses static points of the environment as features for real-time localization and mapping. When there are few available point features, the system is difficult to implement. A feasible solution is to introduce line features. In complex scenarios containing rich line segments, the description of line segments is not strongly differentiated, which can lead to incorrect association of line segment data, thus introducing errors into the system and aggravating the cumulative error of the system. To address this problem, a point-line stereo visual SLAM system incorporating semantic invariants is proposed in this paper. This system improves the accuracy of line feature matching by fusing line features with image semantic invariant information. When defining the error function, the semantic invariant is fused with the reprojection error function, and the semantic constraint is applied to reduce the cumulative error of the poses in the long-term tracking process. Experiments on the Office sequence of the TartanAir dataset and the KITTI dataset show that this system improves the matching accuracy of line features and suppresses the cumulative error of the SLAM system to some extent, and the mean relative pose error (RPE) is 1.38 and 0.0593 m, respectively.

Anti-Müllerian hormone (AMH) is recognized as a reliable marker of ovarian reserve. However, the regulatory mechanism of goose AMH gene remains poorly understood. In the present study, both the full-length coding sequence (CDS) and promoter sequence of goose AMH have been cloned. Its CDS consisted of 2013 nucleotides encoding 670 amino acids and the amino acid sequence contained two structural domain: AMH-N and transforming growth factor beta (TGF-β) domain. The obtained promoter sequence spanned from the −2386 bp to its transcription start site (ATG). Core promoter regions and regulatory elements were identified as well as transcription factors were predicted in its promoter sequence. The luciferase activity was the highest spanning from the −331 to −1 bp by constructing deletion promoter reporter vectors. In CHO cells, the luciferase activity significantly increased by co-expression of AMH and GATA binding protein 4 (GATA-4), while that significantly decreased by mutating the binding sites of GATA-4 located in the −778 and −1477 bp. Results from quantitative real-time polymerase chain reaction (qPCR) indicated that levels of AMH mRNA in geese granulosa layers decreased gradually with the increasing follicular diameter. Taken together, it could be concluded that the transcriptional activity of AMH was activated by GATA-4 to inhibit the development of small follicles in goose.