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Abstract The fiber index including fiber length, width, wall thickness and lumen diameter of Ferrocalamus strictus culms (1, 2, and ≥ 3 years) from Jinping, Mojiang and Lvchun counties of Yunnan Province was determined and the elemental content of the soil was also determined to analyze the fiber characteristics. The average relative fiber index measured for F. strictus culms were fiber length (1.30 mm), width (21.57 μm), slenderness ratio (60.79 μm), wall thickness (4.21 μm), lumen diameter (7.22 μm), and runkel ratio (1.22 μm), which belonged to the range of middle and long fibers. The fiber length increased with the culm age. The proportion of long fiber increased while short fibers decreased along with culm maturing. The fiber morphology did not show a specific trend with the culm height. Fiber length reached the maximum in the bottom portions of the culms. There is a correlation between fiber morphology and soil elements, the content of organic matter, total potassium, total sulfur, total aluminum, total zinc, total iron, total boron, alkali-hydrolyzed nitrogen and available silicon in the soil affects fiber morphology. The content of organic matter, total boron and alkali-hydrolyzed nitrogen in the soil from Mojiang County was largest. Comparatively, the culm fiber in Mojiang County had the best fiber index performance for utilization, since the greatest proportion of medium and long fibers and the optimal distribution of fiber length frequency was obtained from the culms in Mojiang County. This study can provide a theoretical basis for large-scale bamboo forest cultivation and the development and utilization of bamboo culm fiber.

The gut fungi play important roles in human health and are involved in energy metabolism. This study aimed to examine gut mycobiome composition in obese subjects in two geographically different regions in China and to identify specific gut fungi associated with obesity. A total of 217 subjects from two regions with different urbanization levels [Hong Kong (HK): obese, n = 59; lean, n = 59; Kunming (KM): obese, n = 50; lean, n = 49. Mean body mass index (BMI) for obesity = 33.7] were recruited. We performed deep shotgun metagenomic sequencing on fecal samples to compare gut mycobiome composition and trophic functions in lean and obese subjects across these two regions. The gut mycobiome of obese subjects in both HK and KM were altered compared to those of lean subjects, characterized by a decrease in the relative abundance of Nakaseomyces, Schizosaccharomyces pombe, Candida dubliniensis and an increase in the abundance of Lanchanceathermotolerans, Saccharomyces paradox, Parastagonospora nodorum and Myceliophthorathermophila. Reduced fungal – bacterial and fungal – fungal correlations as well as increased negative fungal-bacterial correlations were observed in the gut of obese subjects. Furthermore, the anti-obesity effect of fungus S. pombe was further validated using a mouse model. Supplementing high-fat diet-induced obese mice with the fungus for 12 weeks led to a significant reduction in body weight gain (p

Abstract Pelvic lymph node dissection (PLND) is commonly performed alongside radical prostatectomy. Its primary objective is to determine the lymphatic staging of prostate tumors by removing lymph nodes involved in lymphatic drainage. This aids in guiding subsequent treatment and removing metastatic foci, potentially offering significant therapeutic benefits. Despite varying recommendations from clinical practice guidelines across countries, the actual implementation of PLND is inconsistent, partly due to debates over its therapeutic value. While high-quality evidence supporting the superiority of PLND in oncological outcomes is lacking, its role in increasing surgical time and risk of complications is well-recognized. Despite these concerns, PLND remains the gold standard for lymph node staging in prostate cancer, providing invaluable staging information unattainable by other techniques. This article reviews PLND's scope, guideline perspectives, implementation status, oncologic and non-oncologic outcomes, alternatives, and future research needs.

High-quality genomic DNA extraction is fundamental for the study of gene cloning and expression in plants. Therefore, this study evaluated several methods for extracting genomic DNA from shoots of four Dendrocalamus species to determine the optimal technique. Genomic DNA was extracted using three different methods: a commercial DNA extraction kit method, a modified cetyltrimethylammonium bromide method and a sodium dodecyl sulfate method. A membership function analysis was employed to compare these methods. The results demonstrated that the commercial DNA extraction kit method was the most effective and comprehensive approach for extracting genomic DNA from shoots of four Dendrocalamus species. Furthermore, this study provided valuable insights into optimizing techniques for extracting genomic DNA in other bamboo species.

Silicon (Si) is abundant in the lithosphere, and previous studies have confirmed that silicon plays an important role in plant growth. Higher plants absorb soluble silicon from soil through roots which is deposited in plant tissues mainly in the form of phytoliths. Based on previous studies, the research progress in silicon and phytoliths in the structural protection, enhancement on photosynthesis and transpiration of plants and plant growth and stress resistance was reviewed. Meanwhile, gaps in phytolith research, including phytolith morphology and function, impact of diverse environmental factors coupling with phytoliths, phytolith characteristics at different stages of plant development and phytoliths in regional vegetation are identified. The paper intends to promote the wider application of phytolith research findings and provides reference for further research on phytoliths.

Abstract Necroptosis is a mode of programmed cell death that overcomes apoptotic resistance. The accurate prognosis of cutaneous melanoma is complicated to predict due to tumor heterogeneity. Necroptosis contributes to the regulation of oncogenesis and cancer immunity. We comprehensively investigated different necroptosis patterns by the non-negative matrix factorization (NMF) clustering analysis and explored the relationships among necroptosis patterns, infiltered immune cells, and tumor microenvironment (TME) scores. Two different necroptosis patterns were identified, and the two clusters could predict prognosis and immune landscape. A four-gene signature was successfully constructed and validated its predictive capability of overall survival (OS) in cutaneous melanoma patients. The prognostic value of the signature was further enhanced by incorporating other independent prognostic factors such as age and clinicopathological stages in a nomogram-based prediction model. Patients with lower risk scores tended to have better OS, higher TME score, immune checkpoints, immunophenoscore (IPS), and lower Tumor Immune Dysfunction and Exclusion (TIDE), which indicated better responses to immunotherapy. In addition, the pigmentation score of the high-risk group was visibly higher than those of the low-risk group. In conclusion, the necroptosis-related signature indicated favorable predictive performance in cutaneous melanoma patients, which provides guidance for immunotherapy and provide novel insights into precision medicine.

Under the condition of normalized epidemic, how athletes train and compete well has been in the spotlight. This article reported the symptom, hospitalization and training situation of seven confirmed cases of coronavirus-disease-2019 (COVID-19) among Chinese national teams. Moreover, the paper summarized the experience of Chinese national teams in terms of epidemic prevention and control, treatment of infection, and safe return to play. Through a scientific combination of medication and non-medical treatment, seven athletes were all discharged from the hospital. These discharged athletes underwent strict isolation and scientific training before returning to sports teams. Before returning to play, continuous monitoring of physical and mental condition was required. All seven athletes returned to play safely and performed excellently. As for hosting large-scale sporting events, the entire enclosed-loop management from immigration to competition was proposed in this paper. This study could serve as a standard of epidemic prevention and control, treatment for infection and safe return to play during competition and training around the world.

Bamboo propagation and seed collection are severely limited by a protracted and unpredictable flowering cycle. Dendrocalamus hamiltonii Nees et Arn. ex Munro was renowned for its delectable, bitter-free bamboo shoots, making it an exceptional choice for culinary purposes, which has significant economic value in China. To date, no fruit has been collected, and there are currently no comprehensive studies available on the floral morphology and embryology of D. hamiltonii. The morphological and anatomical characteristics of floral organs were described, and the developmental process of male and female gametophytes was elucidated, through anatomical observation. It was found that the floret of D. hamiltonii was composed of one lemma, palea, pistil, and six stamens, but lacked lodicules. The single ovule, possessing a parietal placenta, was anatropous and featured a double integument. The floral organs showed normal in external morphology, whereas male and female gametophyte development was abnormal, such as pollen grain shrinkage, hollow anther chambers, and underdeveloped ovules, which might be the important factors of its low seed setting rate. This study provided important information on the developmental stages of D. hamiltonii flowers and offered a theoretical basis for understanding the low seed setting rate of bamboo.

The moisture, ash, and silicon content, as well as the phytolith morphotype and concentration in the tissue-cultured, seed-cultured, and grafted seedling leaves of Dendrocalamus brandisii were determined to investigate the differences in silicon uptake and phytolith morphology in the leaves from different rearing methods. The results showed that ash, silicon content, and phytolith concentration were higher in the mature leaves. Tissue-cultured seedlings had a significantly higher moisture content than grafted seedlings. Ash and silicon demonstrated the same order of grafted seedlings > tissue-cultured seedlings > seed-cultured seedlings. The highest phytolith concentration was found in tissue-cultured seedlings. The phytolith morphotypes in D. brandisii seedling leaves raised by different methods were identical and grouped into eight morphotypes. The phytolith assemblage was characterized by a high frequency of bilobate and saddle, accounting for more than 60%, whereas the morphotypes of elongate, blocky, flabellate, and circular phytoliths accounted for the smallest proportion, normally all below 4.5%. The phytolith size demonstrated an increasing trend in the maturing leaves. The sizes of bilobate, saddle, and acute phytoliths expanded the fastest in tissue-cultured seedling leaves, implying rapid growth of the cell in tissue-cultured seedlings. Accordingly, the tissue-cultured seedlings contained more silicon and phytoliths of larger sizes, which could be a better choice of stock supply for establishing large-scale plantations. If the stock of the seed-cultured and grafted seedlings is to be used, silicon fertilizer application is an optimal option to boost seedling growth.

ObjectiveWhile prior reports have characterized visible changes in neuroimaging findings in individuals suffering from sudden sensorineural hearing loss (SSNHL), the utility of regional homogeneity (ReHo) as a means of diagnosing SSNHL has yet to be established. The present study was thus conducted to assess ReHo abnormalities in SSNHL patients and to establish whether these abnormalities offer value as a diagnostic neuroimaging biomarker of SSNHL through a support vector machine (SVM) analysis approach.MethodsResting-state functional magnetic resonance imaging (rs-fMRI) analyses of 27 SSNHL patients and 27 normal controls were conducted, with the resultant imaging data then being analyzed based on a combination of ReHo and SVM approaches.ResultsRelative to normal control individuals, patients diagnosed with SSNHL exhibited significant reductions in ReHo values in the left cerebellum, bilateral inferior temporal gyrus (ITG), left superior temporal pole (STP), right parahippocampal gyrus (PHG), left posterior cingulum cortex (PCC), and right superior frontal gyrus (SFG). SVM analyses suggested that reduced ReHo values in the left cerebellum were associated with high levels of diagnostic accuracy (96.30%, 52/54), sensitivity (92.59%, 25/27), and specificity (100.00%, 27/27) when distinguishing between SSNHL patients and control individuals.ConclusionThese data suggest that SSNHL patients exhibit abnormal resting-state neurological activity, with changes in the ReHo of the left cerebellum offering value as a diagnostic neuroimaging biomarker associated with this condition.

Abstract Background Coronavirus disease 2019 (COVID-19) caused by the enveloped RNA virus SARS-CoV-2 primarily affects the respiratory and gastrointestinal tracts. SARS-CoV-2 was isolated from fecal samples, and active viral replication was reported in human intestinal cells. The human gut also harbors an enormous amount of resident viruses (collectively known as the virome) that play a role in regulating host immunity and disease pathophysiology. Understanding gut virome perturbation that underlies SARS-CoV-2 infection and severity is an unmet need. Methods We enrolled 98 COVID-19 patients with varying disease severity (3 asymptomatic, 53 mild, 34 moderate, 5 severe, 3 critical) and 78 non-COVID-19 controls matched for gender and co-morbidities. All subjects had fecal specimens sampled at inclusion. Blood specimens were collected for COVID-19 patients at admission to test for inflammatory markers and white cell counts. Among COVID-19 cases, 37 (38%) patients had serial fecal samples collected 2 to 3 times per week from time of hospitalization until after discharge. Using shotgun metagenomics sequencing, we sequenced and profiled the fecal RNA and DNA virome. We investigated alterations and longitudinal dynamics of the gut virome in association with disease severity and blood parameters. Results Patients with COVID-19 showed underrepresentation of Pepper mild mottle virus (RNA virus) and multiple bacteriophage lineages (DNA viruses) and enrichment of environment-derived eukaryotic DNA viruses in fecal samples, compared to non-COVID-19 subjects. Such gut virome alterations persisted up to 30 days after disease resolution. Fecal virome in SARS-CoV-2 infection harbored more stress-, inflammation-, and virulence-associated gene encoding capacities including those pertaining to bacteriophage integration, DNA repair, and metabolism and virulence associated with their bacterial host. Baseline fecal abundance of 10 virus species (1 RNA virus, pepper chlorotic spot virus, and 9 DNA virus species) inversely correlated with disease COVID-19 severity. These viruses inversely correlated with blood levels of pro-inflammatory proteins, white cells, and neutrophils. Among the 10 COVID-19 severity-associated DNA virus species, 4 showed inverse correlation with age; 5 showed persistent lower abundance both during disease course and after disease resolution relative to non-COVID-19 subjects. Conclusions Both enteric RNA and DNA virome in COVID-19 patients were different from non-COVID-19 subjects, which persisted after disease resolution of COVID-19. Gut virome may calibrate host immunity and regulate severity to SARS-CoV-2 infection. Our observation that gut viruses inversely correlated with both severity of COVID-19 and host age may partly explain that older subjects are prone to severe and worse COVID-19 outcomes. Altogether, our data highlight the importance of human gut virome in severity and potentially therapeutics of COVID-19. Video Abstract.

This study was aimed at screening potential probiotic candidates to enhance the antimicrobial activity of Artemisia argyi against foodborne pathogens. Ten LAB strains were isolated from natural Artemisia argyi fermentation liquor (AAFL) and assessed for safety and antimicrobial ability. Therein, Lactiplantibacillus plantarum WLPL01, Lacticaseibacillus casei WLCA01, WLCA02, and WLCA03, and Lactobacillus harbiness WLHA01 were further evaluated for their potential probiotic properties (gastrointestinal tolerance and adhesion capacity). The results suggested that L. plantarum WLPL01 exhibited excellent properties and was, therefore, selected as the starter for A. argyi leaves fermentation. Then, L. plantarum WLPL01-fermented AAFL (AAFL-LP) was further investigated for its antimicrobial activity against foodborne pathogens. The results demonstrated that the inhibitory effect of AAFL-LP to foodborne pathogens, such as Listeria monocytogenes CMCC54007, Salmonella Typhimurium ATCC 13311, and Candida albicans ATCC 14053, was enhanced when compared to spontaneously fermented AAFL (AAFL-spontaneous). In addition, an analysis of the whole genome of L. plantarum WLPL01 revealed the presence of 13.9 kb long and 16 plantaricin-encoding loci (pln locus), and the increased antimicrobial activities of AAFL-LP might correlate with the production of bacteriocin. Our results indicate that L. plantarum WLPL01 can be used as a starter for Artemisia argyi fermentation to enhance its antimicrobial activity against foodborne pathogens.

Reasonable management could produce the good growth of bamboo plants. There are few studies in bamboo that integrate phenology and ecophysiological traits; in this case, water relations, photosynthetic rates and carbohydrate content in different organs of Neosinocalamus affinis were determined. The moisture content was easily affected by the local precipitation and showed a similar trend in leaves and branches, with the highest values in July and August, but did not vary significantly between vegetative phenological stages in culms. The emergence and growth of shoots caused an apparent decrease in the moisture content of 1-year culms. NSC content showed a similar trend in culms and branches in classes of different ages and decreased progressively from March through November and then increased in the following months. Net photosynthetic rate and transpiration pull showed the highest values in July and then decreased constantly, which implied that a great deal of water was consumed for photoassimilate synthesis. The net photosynthetic rates of leaves were significantly affected by the water status of culms in the dry season, but correlated significantly only with the leaf water content in the wet season for the sufficient water supply. The transpiration pulls, water potential and sap flow rates revealed the water status of culms better than the water content of culms. The shoot germination and growth of N. affinis were more dependent on the photoassimilate accumulation in the early stage from May to July. There was no real dormancy period for N. affinis due to its apparent photoassimilate accumulation and water flow in January. This suggested that proper irrigation was essential during leaf and branch extension during the dry season.

Abstract Glioblastoma multiforme (GBM) brings serious physical and psychological pain to GBM patients, whose survival rate remains not optimistic. Long noncoding RNAs (lncRNAs) have been reported to participate in the progression of many cancers, including GBM. However, the mechanism and function of long intergenic non-protein coding RNA 1152 (LINC01152) in GBM are still unclear. In our study, we aimed to explore the function and mechanism of LINC01152 in GBM. Then qRT-PCR analysis was implemented to search the expression of RNAs in GBM tissues and cells. Functional assays such as EdU assay, colony formation assay, TUNEL assay and flow cytometry analysis were conducted to estimate GBM cell proliferation and apoptosis. RNA pull down assay, luciferase reporter assay, RIP and ChIP assays were implemented to search the binding between molecules. As a result, we discovered that LINC01152 was upregulated in GBM tissues and cells. LINC01152 and mastermind like transcriptional coactivator 2 (MAML2) could both play the oncogenic part in GBM. Moreover, LINC01152 positively regulated MAML2 in GBM by sponging miR-466 and recruiting SRSF1. In turn, RBPJ/MAML2 transcription complex was found to activate the transcription of LINC01152 in GBM cells. In conclusion, LINC01152 could upregulate the expression of MAML2 to promote tumorigenesis in GBM via Notch signaling pathway.

The Internet of Things is based on a communication network to automatically receive various information from the natural world. It uses intelligent objects with perception, communication and computers to automatically receive various information from nature. All independently managed physical objects are connected to each other to achieve integrated perception, reliable transmission and intelligent processing, and to establish intelligent information between people and objects, and objects and object service systems. This article mainly introduces the impact of industrial agglomeration on the regional economy in a simulated intelligent environment based on machine learning. This paper proposes a method to detect industrial agglomeration index to analyze industrial agglomeration. Through the establishment of the industrial agglomeration index system, the level of integration of the manufacturing industry in our city was objectively analyzed, and the impact of the integration of manufacturing industry on our city was tested empirically. Finally, the relationship between industrial integration and regional economic development was tested. The experimental results in this paper show that industrial integration in a smart environment based on simulation learning has a significant positive impact on regional economic development. Among them, the level of cooperation between the manufacturing service industry and the manufacturing industry increased by 1%, and the level of regional economic development increased by 0.025%.

Abstract Mounting literatures have revealed the crucial effects of long noncoding RNA (lncRNA) in various cancers, including glioma. HNF1A‐AS1, a novel lncRNA, is reported to modulate tumorigenesis and development of multiple cancers. However, the tumorigenic function of lncRNA HNF1A‐AS1 in glioma remains largely unknown. quantitative reverse transcription and polymerase chain reaction and western blot assays were applied to evaluate the expression of relevant mRNAs and proteins. 5‐Ethynyl‐2’‐ deoxyuridine, terminal deoxynucleotidyl transferase dUTP nick‐end labeling, flow cytometry, and transwell assays were conducted for examining the influence of HNF1A‐AS1 on glioma cell functions. The relationship among RNAs was investigated by mechanical experiments. The results demonstrated that HNF1A‐AS1 was predominantly highly expressed in glioma cell lines compared with nontumor glial epithelial cell, which was associated with the stimulation of transcription factor myelocytomatosis oncogene. Knockdown of HNF1A‐AS1 remarkably inhibited glioma cells proliferation, migration, and invasion, while accelerating cell apoptosis in vitro. Mechanically, HNF1A‐AS1 served as a miR‐32‐5p sponge. Moreover, SOX4 was discovered as a target of miR‐32‐5p. Inhibited miR‐32‐5p or upregulated SOX4 could markedly counteract the inhibitory effects of silencing HNF1A‐AS1 on glioma malignant biological behaviors. HNF1A‐AS1 exerted oncogenic property in glioma progression via upregulating miR‐32‐5p–mediated SOX4 expression, suggesting potential novel therapeutic target for future glioma treatment.

Renal ischemia-reperfusion (IR) is frequently observed in patients who are critically ill, yet there are no reliable or effective approaches for the treatment of this condition. Ferroptosis, a form of programmed cell death, is regulated by key genes such as glutathione peroxidase 4 (GPX4) and heme oxygenase-1 (HMOX1) and participates in the injury of renal tubular epithelial cells during IR. This study aimed to investigate the miRNA-mRNA regulatory networks involved in ferroptosis following renal IR. Using bioinformatics analysis, HMOX1 was found to be significantly upregulated during the early stages of renal IR injury, and microRNA-3587 (miR-3587) was identified as a putative regulator of HMOX1. When a miR-3587 inhibitor was applied in a hypoxia-reoxygenation (HR) model system using renal tubular epithelial cells, HO-1 protein (encoded by HMOX1) expression was significantly increased relative to that observed in the HR group, with concomitant increases in GPX4 protein levels, enhanced cell viability, a reduction in malondialdehyde content, decreased Fe2+ level, and the restoration of normal mitochondrial membrane potential. Transmission electron microscopy showed a reduced or absent mitochondrial crest and a damaged mitochondrial outer membrane. Targeting of HMOX1 by miR-3587 was confirmed by luciferase reporter gene assay. In conclusion, these preliminary results indicate that inhibition of miR-3587 promotes HO-1 upregulation, thereby protecting renal tissues from IR-induced ferroptosis.

Bamboo is a plant that may replace plastics and wood as a new green environmental protection material that can be developed. Ferrocalamus strictus is a very special and rare bamboo which is a plant species with extremely small populations (PSESP) endemic to Yunnan, China, and it is listed as a China key national first-class protected wild plant. To explore the effects of extreme climate and environmental changes on this PSESP, we conducted a case study on the response of F. strictus to climate under two extreme future emission scenarios. We combined 29 environmental variables, including the temperature, precipitation, altitude, slope, aspect, soil pH, soil organic carbon content, and mean UV-B radiation of the highest month, to analyze the important environmental factors limiting the distribution of F. strictus and identified the priority conservation areas (PCAs) of F. strictus. Under the two future extreme climate scenarios, the core potential suitable distribution area of F. strictus would decrease by 26%–34% in 2040, 44%–46% in 2060, and 23%–58% in 2080, respectively. Bio18 (precipitation in the warmest quarter) and Bio3 (isotherm) were the two key factors limiting the geographical distribution of F. strictus (the contribution rates were 40.1% and 18.45%, respectively). The results of this study show that F. strictus is in urgent need of protection and PSESPs require more attention.

The gut microbiome comprised of microbes from multiple kingdoms, including bacteria, fungi, and viruses. Emerging evidence suggests that the intestinal fungi (the gut “mycobiome”) play an important role in host immunity and inflammation. Advances in next generation sequencing methods to study the fungi in fecal samples and mucosa tissues have expanded our understanding of gut fungi in intestinal homeostasis and systemic immunity in health and their contribution to different human diseases. In this review, the current status of gut mycobiome in health, early life, and different diseases including inflammatory bowel disease, colorectal cancer, and metabolic diseases were summarized.

Summary: Metal or metal-ion-based batteries represent the mainstream battery technology, in which metal ions either participate in the electrode redox reaction or act as the charge carrier in the electrolyte. The principle guides battery designs and leads to the most widely adopted battery construction of metal-ion acceptor/metal salt in solvent/metal-ion donor. Although these architectures have seen significant progress, there are still many issues with them, including dendrite growth, solid-electrolyte interface corruption, and safety concerns. In this article, the intercalation/deintercalation of organic cations other than metal ions is explored in an all-organic battery composed of organic anode/cathode and pure ionic liquid electrolyte. This metal-free and solvent-free concept has been demonstrated by polyimide/1-ethyl-3-methylimidazolium bis(trifluoromethylsulfonyl)imide/polytriphenylamine full-cell, through which capacity near theoretical value, more than 5,000 cycling life, rate capability up to 200 C, and remarkable low-temperature performance has been achieved. Our work provides an alternate way of developing future efficient, safe, green, and versatile batteries. : Electrochemistry; Electrical Property; Energy Materials Subject Areas: Electrochemistry, Electrical Property, Energy Materials

Non-structural carbohydrate (NSC) dynamics in different bamboo types underlie the different management practices of bamboo plantations. The changes in moisture, soluble sugars, and starch content in response to phenological variations in different types of bamboo were measured to reveal the NSC dynamics in different bamboo forests for high yield. It was reported for the first time that new leaves of monopodial bamboo started sprouting after the completion of branching, while sympodial bamboo extended new leaves and branches simultaneously. NSC accumulation for shooting lasted about eight months for monopodial bamboo but only a short interval of two to three months for sympodial bamboo. The moisture content in different types of bamboo showed a similar trend of increasing from March to July and then decreasing until the coming January. From March to May, particularly in March, irrigation was required for the shooting of monopodial bamboo, whereas the shoot buds in sympodial bamboo showed sprouting, branching, and leafing. Total NSCs in different bamboo types remained relatively high in March, gradually decreased to the lowest in July, and then increased continuously until the coming January. Average soluble sugar and starch contents showed the same trend as the total NSCs in different types of bamboo. During the period from March to July, large amount of photoassimilates were required for shooting and height growth and then for branching and leafing in monopodial bamboo. Abundant photoassimilates were also required for branching, leafing, and shoot bud differentiation, as well as for subsequent shoot germination in sympodial bamboo. Thus, fertilizer application to both monopodial and sympodial bamboo plantations should be scheduled in early May and late July. Given the moisture, soluble sugar, starch, and total NSC content, January was the best season for harvesting monopodial bamboo for high production and future growth, whereas for sympodial bamboo, November was the best season.

In recent years, China's economic development has advanced by leaps and bounds, but the development of China's rural logistics system is still at its primary stage. Some remote areas with inconvenient transportation are still in a state of serious lack or even blank, and due to the high cost of rural logistics delivery services, the rural logistics business of the enterprise also has a profit problem, which limits the development of rural logistics talent innovation to some extent. The purpose of this paper is to study a new influencing factor model of the Internet rural logistics talent innovation mechanism. This paper innovatively proposes countermeasures to improve the innovation of e-commerce practitioners in rural areas. Through research, the author finds that the innovation of rural e-commerce application talents in China is generally low. The key point of the solution lies in how to improve the level of innovation in rural e-commerce application talents. According to the status quo, identify the factors that hinder the innovation and improvement of rural e-commerce application talents. Combined with the great environment of the development of rural e-commerce industry in China, the paper proposes to improve the countermeasures for improving the innovation of rural e-commerce application talents. Improve the current situation of rural e-commerce application talents mediocrity and promote the innovation of rural e-commerce application talents. Fundamentally promote agricultural development and the building of a new socialist countryside. This paper adopts the literature research method based on fuzzy comprehensive evaluation method, system analysis method and the combination of questionnaire survey and interview. Through big data and information science methods for data processing, using a company's Internet rural talent data set to simulate, the results It shows that with the method of this paper, the recognition rate reaches 98%, the speed increases obviously, and it is 20% faster than others.

The physiological function of bamboo shoot sheaths is still unclear. In the present study, we investigated the anatomical and physiological influences of bamboo shoot sheaths on internode elongation by longitudinally striping parts of sheaths. The internodes would bend toward the bare sides during night. The results showed that amounts of water leaked at the cut of shoot sheaths during night, which impeded the increase of water, water pressure and assimilate transport rates, and decreased starch and soluble sugar catabolism in the bare side of the internodes. A higher level of water pressure and sugar metabolism increased the vacuole expansion and promoted the cell expansion in the outer sides as compared to the bare sides. The bending growth of internodes was mainly due to the significant differences in cell expansion, which was led by the difference in water pressure and sugar hydrolysis levels between the inner and outer sides. Bamboo internode elongation mainly relied on the increase of water pressure and soluble sugar concentration. Shoot sheaths played an important role in the rapid growth of bamboo shoots as a controller in water and assimilate transportation. This study gave a new insight into understanding the rapid growth mechanism of bamboo plants.

Strobilurin fungicides have been widely used in agricultural fields for decades. These pesticides are designed to manage fungal pathogens, although their broad-spectrum mode of action also produces non-target impacts. Therefore, the removal of strobilurins from ecosystems has received much attention. Different remediation technologies have been developed to eliminate pesticide residues from soil/water environments, such as photodecomposition, ozonation, adsorption, incineration, and biodegradation. Compared with conventional methods, bioremediation is considered a cost-effective and ecofriendly approach for the removal of pesticide residues. Several strobilurin-degrading microbes and microbial communities have been reported to effectively utilize pesticide residues as a carbon and nitrogen source. The degradation pathways of strobilurins and the fate of several metabolites have been reported. Further in-depth studies based on molecular biology and genetics are needed to elaborate their role in the evolution of novel catabolic pathways and the microbial degradation of strobilurins. The present review summarizes recent progress in strobilurin degradation and comprehensively discusses the potential of strobilurin-degrading microorganisms in the bioremediation of contaminated environments.

Background: Papillary thyroid carcinoma usually shows an excellent prognosis. However, its recurrence or persistence rate is high. In this study, we used bioinformatics to identify autophagy-related genes (ARGs) and establish a novel scoring system for papillary thyroid carcinoma. Methods: We collected ARGs sequencing data of patients with papillary thyroid carcinoma from The Cancer Genome Atlas database. Differentially expressed ARGs were identified by the “Limma” package in R language. After univariate and multivariate Cox regression analysis, an ARG signature was developed. The established prognostic signature was evaluated by Kaplan-Meier curve and time-dependent receiver operating characteristic. Results: A sum of 26 differentially expressed ARGs were identified. Gene set enrichment analysis revealed that several significantly oncological signatures were enriched, such as autophagy, p53 signaling pathway, apoptosis, human cytomegalovirus infection, and platinum drug resistance. After univariate and multivariate analysis, 3 ARGs ( ITPR1 , CCL2 , and CDKN2A ) were selected to develop autophagy-related signature. Patients with high risk had significantly shorter overall survival than those with low risk. The areas under the curve indicated that the signature showed good accuracy of prediction. Conclusions: We established a novel scoring system based on 3 ARGs, which provides a promising tool for the development of personalized therapy.

Aiming at the problems of weak dynamic response and difficulty in diagnosis of early damage of rolling bearings, a diagnosis method for early damage of rolling bearings is proposed. Taking radial rolling bearings as the main research object, the load symmetric structure of deep groove ball bearings is analyzed. Based on the mechanical second-order system theory, the sensor monitoring structure is constructed. The generalized resonance principle is used to identify weak signals, and the fiber Bragg grating is used for signal sensing. The signal is obtained through the fiber Bragg grating high-speed demodulator. When a continuous periodic generalized resonance wave appears in the amplitude–frequency analysis of the signal, and there is a high-frequency resonance frequency, it can be proved that the bearing is faulty. The diagnosis method can effectively avoid the interference of low-frequency signals, the frequency spectrum is pure and there is no electromagnetic interference. It fully shows that the fiber Bragg grating sensor is suitable for the monitoring and diagnosis of the early weak fault of the bearing.

Composite polymer electrolytes (CPEs) incorporate the advantages of solid polymer electrolytes (SPEs) and inorganic solid electrolytes (ISEs), which have shown huge potential in the application of safe lithium-metal batteries (LMBs). Effectively avoiding the agglomeration of inorganic fillers in the polymer matrix during the organic–inorganic mixing process is very important for the properties of the composite electrolyte. Herein, a partial cross-linked PEO-based CPE was prepared by porous vinyl-functionalized silicon (p-V-SiO2) nanoparticles as fillers and poly (ethylene glycol diacrylate) (PEGDA) as cross-linkers. By combining the mechanical rigidity of ceramic fillers and the flexibility of PEO, the as-made electrolyte membranes had excellent mechanical properties. The big special surface area and pore volume of nanoparticles inhibited PEO recrystallization and promoted the dissolution of lithium salt. Chemical bonding improved the interfacial compatibility between organic and inorganic materials and facilitated the homogenization of lithium-ion flow. As a result, the symmetric Li|CPE|Li cells could operate stably over 450 h without a short circuit. All solid Li|LiFePO4 batteries were constructed with this composite electrolyte and showed excellent rate and cycling performances. The first discharge-specific capacity of the assembled battery was 155.1 mA h g−1, and the capacity retention was 91% after operating for 300 cycles at 0.5 C. These results demonstrated that the chemical grafting of porous inorganic materials and cross-linking polymerization can greatly improve the properties of CPEs.

The last few decades have witnessed the rapid growth of research & development on large-scale systems (LSSs) due to the increasing complexity and the growing demand of modern engineering systems. The LSSs can normally be viewed as interconnections of multiple subsystems. Many practical systems can be described by the LSSs such as power systems, multi-robot systems, communication networks, transportation networks, and supply chains. In such large-scale systems, the centralized control framework may become impossible for its implementation. The decentralized and distributed controls have emerged as the attractive control methodologies to handle the scale and interactions of large-scale complex systems. However, the interactions among different subsystems introduce many challenges in the analysis and synthesis of such systems. Therefore, it is of significance to address several fundamental problems regarding real-time analysis, estimation and control of these systems.

Paraquat herbicide has served over five decades to control annual and perennial weeds. Despite agricultural benefits, its toxicity to terrestrial and aquatic environments raises serious concerns. Paraquat cannot rapidly degrade in the environment and is adsorbed in clay lattices that require urgent environmental remediation. Advanced oxidation processes (AOPs) and bioaugmentation techniques have been developed for this purpose. Among various techniques, bioremediation is a cost-effective and eco-friendly approach for pesticide-polluted soils. Though several paraquat-degrading microorganisms have been isolated and characterized, studies about degradation pathways, related functional enzymes and genes are indispensable. This review encircles paraquat removal from contaminated environments through adsorption, photocatalyst degradation, AOPs and microbial degradation. To provide in-depth knowledge, the potential role of paraquat degrading microorganisms in contaminated environments is described as well.

Pyrethroids are broad-spectrum insecticides and presence of chiral carbon differentiates among various forms of pyrethroids. Microbial approaches have emerged as a popular solution to counter pyrethroid toxicity to marine life and mammals. Bacterial and fungal strains can effectively degrade pyrethroids into non-toxic compounds. Different strains of bacteria and fungi such as Bacillus spp., Raoultella ornithinolytica, Psudomonas flourescens, Brevibacterium sp., Acinetobactor sp., Aspergillus sp., Candida sp., Trichoderma sp., and Candia spp., are used for the biodegradation of pyrethroids. Hydrolysis of ester bond by enzyme esterase/carboxyl esterase is the initial step in pyrethroid biodegradation. Esterase is found in bacteria, fungi, insect and mammalian liver microsome cells that indicates its hydrolysis ability in living cells. Biodegradation pattern and detected metabolites reveal microbial consumption of pyrethroids as carbon and nitrogen source. In this review, we aim to explore pyrethroid degrading strains, enzymes and metabolites produced by microbial strains. This review paper covers in-depth knowledge of pyrethroids and recommends possible solutions to minimize their environmental toxicity.

Alzheimer’s disease (AD) is the most common cause of dementia. It is the fifth leading cause of death among elderly people. With high genetic heritability (79%), finding the disease’s causal genes is a crucial step in finding a treatment for AD. Following the International Genomics of Alzheimer’s Project (IGAP), many disease-associated genes have been identified; however, we do not have enough knowledge about how those disease-associated genes affect gene expression and disease-related pathways. We integrated GWAS summary data from IGAP and five different expression-level data by using the transcriptome-wide association study method and identified 15 disease-causal genes under strict multiple testing (α < 0.05), and four genes are newly identified. We identified an additional 29 potential disease-causal genes under a false discovery rate (α < 0.05), and 21 of them are newly identified. Many genes we identified are also associated with an autoimmune disorder.

The disorder distribution of protein in the compartment or organelle leads to many human diseases, including neurodegenerative diseases such as Alzheimer's disease. The prediction of protein subcellular localization play important roles in the understanding of the mechanism of protein function, pathogenes and disease therapy. This paper proposes a novel subcellular localization method by integrating the Convolutional Neural Network (CNN) and eXtreme Gradient Boosting (XGBoost), where CNN acts as a feature extractor to automatically obtain features from the original sequence information and a XGBoost classifier as a recognizer to identify the protein subcellular localization based on the output of the CNN. Experiments are implemented on three protein datasets. The results prove that the CNN-XGBoost method performs better than the general protein subcellular localization methods.

In response to the difficulty of weak detection of early bearing damage, resonance demodulation technology and the principle of fiber Bragg grating sensing strain were combined to design a fiber Bragg grating generalized resonance sensor, which can extract the weak pulse signal of weak detection of early bearing’s early damage from rolling bearing. First, a principle of resonance dynamics of second-order mechanical systems based on fiber Bragg grating and generalized resonance principles is proposed. Second, the basic structure of the sensor is designed. Then, ANSYS finite element simulation is used to analyze the natural frequency of the sensor. Finally, the natural frequency value of the sensor was obtained through experiments. The experimental results of proof-of-principle show that the experimental results are consistent with the theoretical predictions. The theoretical model is accurate, which verifies the feasibility of the sensor.

Purpose: Utilizing the Social Cognitive Theory framework, this research aims to illuminate how self-efficacy acts as a mediating factor, enhancing employability among working university students by fostering essential capabilities in digital leadership and intercultural competence. Design/methodology/approach: Quantitative study with 206 working undergraduates from an Indonesian university. Questionnaires and structural equation modeling (SEM) with SPSS and SmartPLS were used. Findings: Findings showed that working undergraduate students possess a high level of digital leadership capability and intercultural competence and tend to display increased confidence in their ability to perform challenging tasks and attain anticipated outcomes, thereby enhancing their employability. The integration of these skills, coupled with a solid self-efficacy belief, emerges as a powerful combination in the modern employment landscape in the digital transformation and Artificial Intelligence age. Research limitations/implications: This research is conducted in one university in Indonesia, potentially limiting its generalizability. Future studies could expand its regions to multiple universities across different countries for a more comprehensive implication. Practical implications: This research answered the call for strategies from the university level on enhancing the development of essential competencies for working undergraduate students' employability. It further highlights the necessity for shaping the curriculum to prioritize digital leadership capability, intercultural competence, and the cultivation of self-efficacy among working students. Social implications: This insight of the research has profound social implications, indicating that educational institutions must adapt their curricula to prioritize these competencies. By doing so, universities can better prepare students to navigate the complexities of the modern workplace, thereby contributing to a workforce that is not only technologically proficient but also culturally adept and resilient in the face of challenges. This adaptation is essential for fostering a generation of graduates who are well-equipped to contribute positively to a diverse and rapidly evolving labor market, ultimately benefiting both the individuals and the wider society by promoting economic development and cultural understanding. Originality/value: Adopting a quantitative approach, this research offers a data-driven lens of employability determinants, deepening the understanding of how intertwined competencies shape employment outcomes in the AI age.

Dendrocalamus brandisii is an edible bamboo species found in Southwest China and South Asia. However, there is limited information about the anatomical and chemical information of its culms for its utilization and processing. In this paper, the anatomical and chemical properties of different age culms were determined. There are three vascular bundle types found in its culms. The radial length-to-tangential diameter ratio of vascular bundles varied with culm zone but did not vary with age. The outer diameter of metaxylem vessels showed a similar trend. The fiber length (L), wall thickness (W), and Runkel ratio increased with age, while the fiber length-to-outer diameter ratio (L/D) and lumen diameter (Ld) decreased with age. The chemical properties of D. brandisii also differed with age class and height. The holocellulose and ash content decreased from age 1 to 2 years and then increased at year 3. The acid-insoluble lignin, alcohol-toluene extractives, and silica contents increased with age class, whereas the acid-soluble lignin exhibited the opposite trend. The fiber length and L/D values of D. brandisii suggest it would be suitable material for pulp fibers, but its lignin content was relatively high compared with other bamboo species.

The inhomogeneous distribution of parallel pipe flow leads to difficulty in the efficient and reliable operation of fluid power equipment. In view of this, a new type of rectifier nozzle has been proposed in parallel pipelines. Numerical simulation and experimental studies were used to reveal the influence of the rectification nozzle on the flow distribution characteristics. The hydraulic characteristics of the parallel pipelines with and without rectifier nozzles were compared and analyzed. The effects of the temperature and inlet flow on the flow uniformity were studied. The results showed that the initial temperature had little effect on the flow distribution of parallel pipelines, and the flow rates of the branches were not much different. The inlet flow had great influence on the distribution characteristics of the parallel pipelines, but the rectifier nozzles changed the local resistance structure and pressure distribution at the shunt, thereby improving the non-uniformity of the flow distribution of the parallel pipelines, and the maximum difference between the two pipes was optimized from 28.89 t/h (20.3%) to 2.2 t/h (1.5%). The rectifying nozzle could distort the flow field of each branch during the split, making the distribution of flow rate and flow state more uniform and stable. At high inlet fluid temperatures, cavitation could occur under the pressure drop of the nozzle, and partial cavitation had little effect on the flow distribution.

D-phenothrin is one of the most popular pyrethroid insecticides for its broad spectrum and high insecticidal activity. However, continuous use of D-phenothrin has resulted in serious environmental contamination and raised public concern about its impact on human health. Biodegradation of D-phenothrin has never been investigated and its metabolic behaviors remain unknown. Here, a novel bacterial strain P31 was isolated from active sludge, which completely degraded (100%) D-phenothrin at 50 mg⋅L-1 in 72 h. Based on the morphology, 16S rRNA gene and Biolog tests, the strain was identified as Pseudomonas fulva. Biodegradation conditions were optimized as 29.5°C and pH 7.3 by utilizing response surface methodology. Strain P31 depicted high tolerance and strong D-phenothrin degradation ability through hydrolysis pathway. Strain P31 degraded D-phenothrin at inhibition constant (Ki) of 482.1673 mg⋅L-1 and maximum specific degradation constant (qmax) of 0.0455 h-1 whereas critical inhibitor concentration remained as 41.1189 mg⋅L-1. The 3-Phenoxybenzaldehyde and 1,2-benzenedicarboxylic butyl dacyl ester were identified as the major intermediate metabolites of D-phenothrin degradation pathway through high-performance liquid chromatography and gas chromatography-mass spectrometry. Bioaugmentation of D-phenothrin-contaminated soils with strain P31 dramatically enhanced its degradation, and over 75% of D-phenothrin was removed from soils within 10 days. Moreover, the strain illustrated a remarkable capacity to degrade other synthetic pyrethroids, including permethrin, cyhalothrin, β-cypermethrin, deltamethrin, fenpropathrin, and bifenthrin, exhibiting great potential in bioremediation of pyrethroid-contaminated environment.

Persistent use of permethrin has resulted in its ubiquitous presence as a contaminant in surface streams and soils, yet little is known about the kinetics and metabolic behaviors of this pesticide. In this study, a novel bacterial strain Acinetobacter baumannii ZH-14 utilizing permethrin via partial hydrolysis pathways was isolated from sewage sludge. Response surface methodology based on Box-Behnken design of cultural conditions was used for optimization resulting in 100% degradation of permethrin (50 mg·L−1) within 72 h. Strain ZH-14 degraded permethrin up to a concentration of 800 mg·L−1. Biodegradation kinetics analysis indicated that permethrin degradation by this strain was concentration dependent, with a maximum specific degradation rate, half-saturation constant, and inhibition constant of 0.0454 h−1, 4.7912 mg·L−1, and 367.2165 mg·L−1, respectively. High-performance liquid chromatography and gas chromatography-mass spectrometry identified 3-phenoxybenzenemethanol and 3-phenoxybenzaldehyde as the major intermediate metabolites of the permethrin degradation pathway. Bioaugmentation of permethrin-contaminated soils with strain ZH-14 significantly enhanced degradation, and over 85% of permethrin was degraded within 9 days with the degradation process following the first-order kinetic model. In addition to degradation of permethrin, strain ZH-14 was capable of degrading a large range of synthetic pyrethroids such as deltamethrin, bifenthrin, fenpropathrin, cyhalothrin, and beta-cypermethrin which are also widely used pesticides with environmental contamination problems, suggesting the promising potentials of A. baumannii ZH-14 in bioremediation of pyrethroid-contaminated terrestrial and aquatic environments.

The chemical properties and fiber morphology of Fargesia fungosa at different culm ages and height portions were investigated. The variations in moisture, ash, SiO2, and toluene–alcohol extractive contents with culm ages were greater than they were with heights. The holocellulose varied neither significantly with age nor with height. The fiber length, width, length-to-width ratio, and wall thickness of F. fungosa increased with culm ages. Meanwhile, the middle portions of culms at all age classes had the highest values of fiber length and width. The lumen diameter decreased, whereas the wall-lumen ratio increased, with increasing bamboo ages and heights. The 3-year-old culms of F. fungosa are suitable for pulp and papermaking based on their fine chemical properties and fiber morphology.

This paper aims at solving the problem of explosion proof in measurement of thermal gas flow using electronic sensor by presenting a new type of flow sensor by optical fiber heating. A measuring unit based on fiber Bragg grating (FBG) for fluid temperature and a unit for heat dissipation are designed to replace the traditional electronic sensors. The light in C band from the amplified spontaneous emission (ASE) light source is split, with one part used to heat the absorbing coating and the other part used in the signal processing unit. In the heating unit, an absorbing coating is introduced to replace the traditional resistance heating module to minimize the risk of explosion. The measurement results demonstrate a fine consistency between the flow and temperature difference in simulation. The method to enhance the measurement resolution of flow is also discussed.