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Figure S1. Peak analyses of Twist1 ChIP-Seq Data Sets. (A) Heat map view of signals observed in the presence of Twist1. (B) Density graph of the Twist1 peak locations in relation to the nearest transcriptional start sites (TSS). The x-axis represents the distance from the peak center to the nearest TSS, where zero is the position of the TSS. The y-axis represents the proportion of detected bindings that are located within each distance interval. (C) Twist1 ChIP-seq peak density on the Cul2.

Table S1．The plasmids information. Table S2．The primers of overexpressed vectors and siRNA. Table S3．The primers of RT-PCR. Table S4．Clinical characteristics of the patients used for developing PDTX models. Table S5．The sequences of circRNAs probe for FISH.

Twist is a critical epithelial–mesenchymal transition (EMT)–inducing transcription factor that increases expression of vimentin. How Twist1 regulates this expression remains unclear. Here, we report that Twist1 regulates Cullin2 (Cul2) circular RNA to increase expression of vimentin in EMT. Twist1 bound the Cul2 promoter to activate its transcription and to selectively promote expression of Cul2 circular RNA (circ-10720), but not mRNA. circ-10720 positively correlated with Twist1, tumor malignance, and poor prognosis in hepatocellular carcinoma (HCC). Twist1 promoted vimentin expression by increasing levels of circ-10720, which can absorb miRNAs that target vimentin. circ-10720 knockdown counteracted the tumor-promoting activity of Twist1 in vitro and in patient-derived xenograft and diethylnitrosamine-induced TetOn-Twist1 transgenic mouse HCC models. These data unveil a mechanism by which Twist1 regulates vimentin during EMT. They also provide potential therapeutic targets for HCC treatment and provide new insight for circular RNA (circRNA)-based diagnostic and therapeutic strategies.Significance: A circRNA-based mechanism drives Twist1-mediated regulation of vimentin during EMT and provides potential therapeutic targets for treatment of HCC.Graphical Abstract: http://cancerres.aacrjournals.org/content/canres/78/15/4150/F1.large.jpg. Cancer Res; 78(15); 4150–62. ©2018 AACR.

To investigate the clinical features and laboratory characteristics of primary autoimmune hemolytic anemia (AIHA) patients with negative results of direct antiglobulin test (DAT) by tube test but positive results by microcolumn gel assay, in order to provide references for the diagnosis of these patients.59 patients diagnosed with primary AIHA in our hospital from January 2015 to December 2020 were retrospectively analyzed. According to the results of tube test and microcolumn gel assay, the cases were divided into 3 groups, and the clinical and laboratory characteristics of each group were compared.The cases were grouped as follows: Group I, cases with negative results by both methods of DAT (n=5); Group II, cases with negative results by tube test but positive results by microcolumn gel assay (n=26); Group III, cases with positive results by both methods of DAT (n=28). There was no significant difference in age and sex between Group II and other groups, whereas the positive rate of anti-IgG + anti-C3d of Group II was lower than that in Group III (P=0.015). The main clinical manifestations of Group II were chest tightness, shortness of breath, fatigue, as well as yellow skin and sclera or dark urine, but the incidence rate of these symptoms was not significantly different from other groups. Anemia related indexes in Group II such as red blood cell (RBC) count and hemoglobin (Hb) were lower than the reference intervals, but there was no significant difference compared with other groups. Hemolysis related indexes in Group II such as reticulocyte (Ret) ratio, indirect bilirubin (IBIL), lactate dehydrogenase (LDH) and free-hemoglobin (F-Hb) were higher than the reference intervals, and the latter two items were signficantly higher than those in Group I (P=0.031 and P=0.036). Serum complement C3 and C4 in Group II were higher than those in Group III (P=0.010 and P=0.037).Anemia severity of primary AIHA patients who were negative of DAT by tube test but positive by microcolumn gel assay was similar to those with negative or positive results by both DAT methods, but the mechanism and degree of complement system involved in hemolysis might be different. Results above may be helpful for laboratory diagnosis of this kind of patients.DAT试管法阴性而微柱凝胶法阳性的原发自身免疫性溶血性贫血患者的临床与实验室特征.探讨直接抗人球蛋白试验（DAT）试管法阴性而微柱凝胶法阳性的原发自身免疫性溶血性贫血（AIHA）患者的临床与实验室特征，为此类患者的诊断提供借鉴。.回顾性分析2015年1月至2020年12月在本院就诊的原发AIHA患者59例。根据DAT试管法和微柱凝胶法结果将研究对象分为3组，比较各组的临床与实验室特征。.将研究对象分为DAT两方法均阴性组（Ⅰ组，n=5）、DAT试管法阴性而微柱凝胶法阳性组（Ⅱ组，n=26）、DAT两方法均阳性组（Ⅲ组，n=28）。Ⅱ组与其他组别相比，年龄、性别组成均无显著差异，DAT微柱凝胶法抗IgG+抗C3d阳性比例低于Ⅲ组（P=0.015）。Ⅱ组临床表现以胸闷、憋气、乏力等贫血相关症状以及皮肤、巩膜黄染或尿色加深等溶血相关症状为主，但发生率与其他组别无显著差异。Ⅱ组的红细胞数（RBC）、血红蛋白（Hb）等贫血相关指标低于参考范围，但与Ⅰ组、Ⅲ组相比差异无统计学意义。Ⅱ组的网织红细胞（Ret）比率、间接胆红素（IBIL）、乳酸脱氢酶（LDH）、游离血红蛋白（F-Hb）等溶血相关指标高于参考范围，其中后2项显著高于Ⅰ组（P=0.031和P=0.036）。Ⅱ组的血清补体C3、C4水平均高于Ⅲ组（P=0.010和P=0.037）。.DAT试管法阴性而微柱凝胶法阳性的原发AIHA患者与两方法均阴性和均阳性患者相比，贫血程度接近，但补体系统参与溶血的机制和程度可能不同。这一结果可能对此类患者的实验室诊断与鉴别提供帮助。.

Biodiesel is one of the most important bioenergy products. But the feedstock is the bottleneck of its production. In order to mine potential feedstock from the natural resources, a wild survey was conducted in 10 provinces or cities of North China, and 952 seed samples, which came from 587 species of 102 families, were collected for analysis of oil content, oil composition, and chemical properties. The result showed that there are 23 families with the average oil contents higher than 20%, and families of Lauraceae, Magnoliaceae, Celastraceae, Brassicaceae, Labiatae, Sapindaceae are dominant families rich in oil. The fatty acid composition of various vegetable oils is very different, showing the characteristics of different families, genera, and species. Erucic acid was mainly found in the Cruciferae；Pinolenic acid and taxoleic acid was mainly found in the Pinaceae; Oleic acid was mainly found in the Euphorbiaceae. The average iodine value in 81.4% of the families is less than 120 g/100g. Families with an acid value of less than 10 are mainly distributed in Rosaceae, Leguminosae, Cruciferae, Labiatae, Compositae, Liliaceae, Aceraceae, Rhamnaceae, Solanaceae, Cannabaceae, Malvacea, Oleaceae, Cornaceae, Sapindaceae, etc. The saponification value is concentrated between 140 and 220 mg/g in 344 species (62.66%). In 84 species (60.87%), the predicted cetane number is higher than 49, mainly distributed in the families of Rosaceae, Anacardiaceae, Betulaceae, Fagaceae, Oleaceae, Umbelliferae, Theaceae, Celastraceae, etc. These results will help in developing potential biodiesel feedstock, also provide a guide for development of specific oil industry.

To observe the difference in clinical efficacy between acupuncture with point selection based on syndrome differentiation along the meridians and acupuncture at non-meridian and non-acupoint points for functional dyspepsia (FD). A total of 74 FD patients were randomized into an observation group and a control group, with 37 cases in each group. Both groups received acupuncture treatment. Zusanli (ST 36) and Neiguan (PC 6) were selected in the observation group, with Taichong (LR 3) and Neiting (ST 44) added for excess syndrome, and Gongsun (SP 4) and Yinlingquan (SP 9) added for deficiency syndrome. Four non-meridian and non-acupoint points were selected in the control group. The treatments in both groups were performed once a day with a 2-day break after 5 consecutive treatments, which constituted one treatment course. A total of 4 courses were performed. The scores of Nepean dyspepsia index (NDI) and Leeds dyspepsia questionnaire (LDQ) were recorded before and after treatment, and during follow-up (8, 12, 16, 20 and 24 weeks after recruitment) to assess the clinical efficacy. The NDI scores in the two groups after treatment and at each time point during follow-up were higher than those before treatment (all P

Currently, developing supercapacitors with robust cycle stability and suitability for wide-temperature-range operations is still a huge challenge. In the present work, few-layer hexagonal boron nitride nanosheets (h-BNNSs) with a thickness of 2–4 atomic layers were fabricated via vacuum freeze-drying and nitridation. Then, the h-BNNSs/reduced graphene oxide (rGO) composite were further prepared using a hydrothermal method. Due to the combination of two two-dimensional (2D) van der Waals-bonded materials, the as-prepared h-BNNSs/rGO electrode exhibited robustness to wide-temperature-range operations from −10 to 50°C. When the electrodes worked in a neutral aqueous electrolyte (1 M Na2SO4), they showed a great stable cycling performance with almost 107% reservation of the initial capacitance at 0°C and 111% at 50°C for 5000 charge—discharge cycles.

By using in-situ synthesis of polythioamide (PTA) on activated carbon (AC), a polythioamide-modified activated carbon-based adsorbent (AC-PTA) was successfully prepared and used to study the selective adsorption effect and mechanism of Au(Ⅲ) in wastewater. The results showed that AC-PTA exhibited excellent selective adsorption to Au(Ⅲ) in the coexisting solution of multiple metal ions in a wide pH range (5.0). The adsorption effect for Au(Ⅲ) was the best at a pH of 2 and 3; the concentration of residue Au(Ⅲ) was less than 0.1 mg·L

With 8-(methyltosylaminoethynyl)-1-naphthyl (MTAEN) glycoside as donors, a novel and efficient glycosylation protocol has been established. The MTAEN glycosylation protocol exhibits the merits of shelf-stable donors, mild catalytic promotion conditions, considerably extended substrate scope encompassing both free alcohols, silylated alcohols, nucleobases, primary amides, and

Based on the quasi-two-dimensional (2D) field model of long primary double-sided linear induction motor (LPDLIM), an improved equivalent circuit model is proposed. First, the traditional equivalent circuit of LPDLIM is reviewed. Second, the skin effect correction coefficients for the secondary equivalent resistance and excitation reactance, and the secondary leakage inductance are derived. Moreover, an improved equivalent circuit model for LPDLIMs is presented, in which the leakage reactance of the secondary is considered, and the excitation reactance and secondary resistance are modified by the correction coefficients independently. Then, the slip frequency characteristics of various effect forces and variations of forces under different operations and mechanical air gap width are presented. Finally, the calculated forces by the proposed equivalent circuit are validated by the finite element method results and also compared with that of the traditional equivalent circuit model.

The purpose of this single-center, randomized, open, two-period, two-sequence crossover, single-dose administration, bioequivalence research was to evaluate the bioequivalence and safety of the generic formulations of metformin hydrochloride sustained-release (MH-SR) 500 mg tablets (test preparation [T]: YuantangSubjects received 500 mg T/R in each period, with a 7-day washout period. Venous blood samples of 4 mL each were collected from each subject 19 times spanning predose (0 h) to 36 h postdose. The metformin concentration in deproteinized plasma was determined by high-performance liquid chromatography-tandem mass spectrometry. Bioequivalence (80.00-125.00%) was assessed by adjusted geometric mean ratios (GMRs) and two-sided 90% confidence intervals (CIs) of the area under the curve (AUC) and maximum concentration (CThirty-four volunteers completed the clinical study. The 90% CIs (96.12-105.44% for AUC from time zero to the time of the last measurable concentration [AUCYuantang

This study aims to explore the safety and efficacy of growth hormone (GH) therapy by retrospectively analyzing the changes of insulin-like growth factor-1 (IGF-1) Z-scores in children with short stature after treatment with GH. The etiology of 104 children with short stature was classified according to the GH stimulation test and IGF-1 levels: (1) growth hormone deficiency (GHD); (2) mild growth hormone deficiency (M-GHD); (3) idiopathic short stature (ISS); (4) GH insensitivity syndrome (GHIS). In addition, all patients were treated with recombinant human growth hormone (rhGH) for 12 months, and the growth rate (Gv), height, body mass, bone age, height standard deviation scores (HtSDS), IGF-1 and adverse reactions were compared among these three groups before and after treatment. The height, body mass, Gv and HtSDS were significantly higher in each group compared with those before treatment. Furthermore, the Z-score of IGF-1 significantly increased after 1 month of GH treatment and was positively correlated with the dosage of GH. Moreover, the difference in standard deviation score was significantly positively correlated with the increase in standard deviation score of IGF-1. The detection of the GH-IGF-1 axis function can be carried out for the etiologic diagnosis of short stature. IGF-1 increased after rhGH treatment, and IGF-1 level was correlated to the time of therapy and dosage of GH. IGF-1-based GH dosing targeted to age- and gender-adjusted means may save medical costs and offer a more dose-sparing and potentially safer mode of therapy compared with traditional weight-based dosing.

There remains controversy regarding whether the growth charts constructed from data of term infants, such as those produced by the World Health Organization (WHO) standards, can appropriately evaluate the postnatal growth of preterm infants. This retrospective cohort study, conducted in the First Affiliated Hospital of Shandong First Medical University in Jinan China, aimed to compare the postnatal growth charts of singleton preterm and term infants using WHO standards at 40–160 weeks postmenstrual age (PMA). A total of 5,459 and 15,185 sets of longitudinal measurements [length/height, weight, head circumference (HC), and body mass index (BMI)] from birth to 160 weeks PMA were used to construct growth charts for 559 singleton preterm (mean PMA at birth, 33.84 weeks) and 1,596 singleton term infants (born at 40 weeks PMA), respectively, using the Generalized Additive Models for Location, Scale, and Shape (GAMLSS) method. Z-scores (prematurity corrected) were calculated using WHO Anthro software. Compared to WHO standards, all parameters of preterm infants were increased, especially in terms of length/height and weight; the gap between the two almost spanned two adjacent centile curves. Compared to term controls, the length/height, weight, and BMI of preterm infants were higher at 40 weeks PMA, surpassed by term infants at 52–64 weeks PMA, and quite consistent thereafter. The HC of preterm infants at 40–160 weeks PMA was quite consistent with both term controls and the WHO standards. The Z-scores for length/height, weight, and BMI of preterm infants relative to the WHO standards gradually decreased from 1.20, 1.13, and 0.74 at 40–44 weeks PMA to 0.67, 0.42, and 0.03 at 132–160 weeks PMA, respectively; Z-scores for HC of preterm infants rapidly decreased from 0.73 to 0.29 at 40–50 weeks PMA, and then fluctuated in the range of 0.08–0.23 at 50–160 weeks PMA. Preterm infants had higher growth trajectories than the WHO standards and similar but not identical trajectories to term infants during the first 2 years of life. These findings reemphasize the necessity of constructing local growth charts for Chinese singleton preterm infants.

Practical use of heterogeneous Fenton-like catalysis is inhibited by poor mass transfer and slow conversion of Fe(Ⅲ) to Fe(Ⅱ). In this study, we prepared a novel iron-copper bimetallic organic framework (MIL-101 (Fe,Cu)) using the solvothermal method, and carefully investigated its interfacial characters, catalytic efficacy toward dyes with methylene blue as a model pollutant, and the catalytic activating mechanisms involved in it. The MIL-101(Fe,Cu) exhibited a three-dimensional octahedral shape with a complete crystal structure. The specific BET surface area and average pore size were determined to be as high as 667.2 m

Mn(Ⅱ)-oxidizing microorganisms can catalytically increase the oxidation rate of divalent manganese by several orders of magnitude, and affect the valence state and fate of elemental manganese. In addition to Mn(Ⅱ)-oxidization by a single microbial strain, our previous studies revealed that interspecies interactions between two bacterial strains (

In this paper, a new rotation-magnified viscoelastic damper (RMVD) was developed. The damper applying the lever principle amplified the relatively small angular deformation manyfold at the beam-column joints, increased the shear deformation and hysteretic energy dissipation of viscoelastic materials to achieve an ideal energy dissipation effect. Firstly, the basic structure and working principle of the RMVD were introduced, and the quality index of high damping butyl rubber was discussed through the uniaxial tensile test and shear test. Secondly, the physical model of a new rotation-magnified viscoelastic damper was built, and its hysteresis curve was obtained through experiments. Additionally, the mechanical properties were compared with those of the traditional non-amplification function angle damper. Then, according to the structural characteristics and experimental results of the RMVD, the mechanical models were proposed to conduct a numerical simulation. Finally, the dynamic time-history analysis of the steel frame structure with the new damper installed at the beam-column joints was performed, and the results showed that the RMVD has a better damping control effect.

Currently, the construction process of large offshore platforms worldwide involves building the topside and hull separately and then having them integrated. The jacking closure scheme by overlapped support tower bearing the topside is a new integration method, which has a great advantage over other traditional methods in vertical bearing capacity. But its weak bearing capacity to lateral forces like wind load limits the jacking height. Moreover, few engineering examples and academic researches also cause insufficient understanding of it. Therefore, this paper, based on the first deep-water semi-submersible platform with the10,000-ton oil storage worldwide, i.e. “Deep Sea No. 1” energy station, aims to evaluate the wind-resistant performance during the jacking closure process. Firstly, the background and the jacking closure scheme of this project are introduced in detail. Secondly, the finite element models (FEMs) of the jacking system are established according to the overlapped characteristics of jacking towers and the corresponding failure criteria. Then, the wind-induced vibration response is simulated, and the static pushover is conducted to evaluate the ultimate bearing capacity and its influencing factors. The failure criteria are checked using ANSYS parameter design language. It is found that “the occurrence of tensile stress on the contact surface” is the first and main failure mode of the structure. Additionally, the results also reveal that the strand cables can not only improve the ultimate bearing capacity of the structure but also change its weak direction. However, the improvement is gradually weakened with the increase of jacking height. In contrast, the contribution of the bracing pipes to the structural bearing capacity is always significant. These findings can be used to develop a more reasonable closure plan.

In recent years, improving the performance of catalysts through defect modulation has attracted extensive attention. However, the impact of defect rearrangement on Fenton-like photocatalytic reactions has not been studied. In this study, Fe-containing polyoxometalate molecules were grafted onto the surface of defective TiO

Objective: To analyze the modern research and application of Flos Puerariae, and lay a foundation for the comprehensive utilization of Flos Puerariae in the future. Methods: The relevant literatures on Flos Puerariae were screened out on the knowledge network for nearly 20 years. Through analysis and summary, the pharmacological effects, chemical components and attending diseases of Flos Puerariae were summarized. Results: The main constituents of Pueraria lobata were flavonoids, alkaloids and saponins of Flos Puerariae, which had pharmacological effects such as hangover, liver and inflammation, and elimination of free radicals. The search for "Flos Puerariae" on the Internet was conducted from 1998.1 to 2018.10. There are 661 related literatures, including 499 journal articles, and 378 of them are finally selected. Through statistical induction, GeFlos Puerariae is clinically used for diseases such as drunkenness, alcoholism, alcoholic liver disease, etc., and has good curative effect; Conclusion: The existing pharmacological effects of Flos Puerariae can better serve the clinic. The compatibility of Flos Puerariae and other traditional Chinese medicines enhances the therapeutic effect, provides theoretical guidance for some complex diseases, and provides experimental basis for clinical application.

Currently, the construction process of large offshore platforms worldwide involves building the topside and hull separately and then having them integrated. Jacking technology is emerging as a new integration method more suitable for offshore platform construction for its higher tonnage and larger scales compared with the lifting technology. Conventionally, an overlapped support tower (OST) is adopted to jack the topside to a predetermined height. However, with the OST’s increasing jacking height, the wind load effect becomes more significant, which in turn limits the OST height and applications. In this paper, failure criteria were developed for the OST based on the load types and structural characteristics of easy assembly and disassembly. Then a simplified method to simulate the OST was proposed. In addition, a finite element model (FEM) of the entire jacking system with the topside and OST (including 6 jacking towers, 8 strand cables and 23 bracing pipes) was established, and wind-induced vibration analysis was performed to ensure safety of the jacking system. The results show that the addition of the topside increases the torsional effect of the jacking system; the unfavorable wind angles are 0° and 15°, which should be paid much attention during the jacking operation. The recommended wind-induced vibration coefficients for the OST and topside are 3.0 and 4.0, respectively. The proposed method and simulation results of this study provide a reference for the jacking and integration design of similar offshore platforms.

// Li Shang 1, * , Jia-Jie Hao 1, * , Xue-Ke Zhao 3, * , Jian-Zhong He 4, * , Zhi-Zhou Shi 1 , Hui-Juan Liu 5 , Li-Fei Wu 6 , Yan-Yi Jiang 1 , Feng Shi 1 , Hai Yang 1 , Yu Zhang 1 , Yi-Zhen Liu 1 , Tong-Tong Zhang 1 , Xin Xu 1 , Yan Cai 1 , Xue-Mei Jia 5 , Min Li 6 , Qi-Min Zhan 1 , En-Min Li 4 , Li-Dong Wang 3 , Wen-Qiang Wei 2 , Ming-Rong Wang 1 1 State Key Laboratory of Molecular Oncology, Cancer Institute (Hospital), Peking Union Medical College and Chinese Academy of Medical Sciences, Beijing 100021, China 2 Department of Cancer Epidemiology, Cancer Institute (Hospital), Peking Union Medical College and Chinese Academy of Medical Sciences, Beijing 100021, China 3 Henan Key Laboratory for Esophageal Cancer Research of the First Affiliated Hospital, Zhengzhou University, Zhengzhou 450000, China 4 Department of Biochemistry and Molecular Biology, Shantou University Medical College, Shantou 515041, China 5 Department of Histology and Embryology, Anhui Medical University, Hefei 230032, China 6 Department of Gastroenterology, Anqing City Hospital, Affiliated Anqing Hospital of Anhui Medical University, Anqing 246003, China * These authors have contributed equally to this work Correspondence to: Ming-Rong Wang, e-mail: wangmr2015@126.com Wen-Qiang Wei, e-mail: weiwq@cicams.ac.cn Li-Dong Wang, e-mail: ldwang2007@126.com En-Min Li, e-mail: nmli@stu.edu.cn Keywords: ANO1, esophageal squamous cell carcinoma, precancerous lesions, biomarker, prognosis Received: September 17, 2015 Accepted: March 01, 2016 Published: March 21, 2016 ABSTRACT Objectives: Anoctamin 1 (ANO1) has been found to be overexpressed in esophageal squamous cell carcinoma (ESCC) in our previous study. Herein we showed the clinical relevance of ANO1 alterations with ESCC and esophageal precancerous lesion progression. Results: ANO1 was detected in 38.1% (109/286) and 25.4% (77/303) of tumors in the two cohorts, but in none of morphologically normal operative margin tissues. ANO1 expression was significantly associated with a shorter overall survival (OS), especially in patients with moderately differentiated and stage IIA tumors. In 499 iodine-unstained biopsies from the endoscopic screening cohort in 2005-2007, all the 72 pathologically normal epithelial mucosa presented negative immunostaining, whereas ANO1 expression was observed in 3/11 tumors and 5/231 intraepithelial lesions. 7/8 ANO1-positive cases had developed unfavorable outcomes revealed by endoscopic follow-up in 2012. Analysis of another independent cohort of 148 intraepithelial lesions further confirmed the correlation between ANO1 expression and progression of precancerous lesions. 3/4 intraepithelial lesions with ANO1 expression had developed ESCC within 4-9 years after the initial endoscopic examination. Methods: Immunohistochemistry (IHC) was performed to examine ANO1 expression in surgical ESCC specimens and two independent cohorts of esophageal biopsies from endoscopic screening in high-incidence area of ESCC in northern China. Association between ANO1 expression, clinico-pathologic parameters, and the impact on overall survival was analyzed. Conclusions: Positive ANO1 is a promising biomarker to predict the unfavorable outcome for ESCC patients. More importantly, it can predict disease progression of precancerous lesions.

Ethnopharmacological relevance Herbaceous peony (Paeonia lactiflora Pall.) flower has been used widely in dietotherapy in China and other countries. It has good ethnopharmacological value in the treatment of various metabolic diseases. However, the molecular mechanisms by which it lowers serum uric acid are unknown. The development of pharmaceutical resources is very important. Here, we sought to elucidate the mode of action of herbaceous peony in terms of reducing uric acid levels. Aim of the study: In the present research, the effects of the total glucosides of herbaceous peony flower were investigated in a rat hyperuricaemia model. Another aim of the study was to clarify the mechanism by which herbaceous peony flower (TGPF) lowers serum uric acid levels. Materials and methods A hyperuricaemic rat model was induced via intragastric administration of 100 mg/kg adenine and 250 mg/kg ethambutol hydrochloride (EH) for 23 d. Then TongFengShu 600 mg/kg, allopurinol 42 mg/kg, or TGPF (50 mg/kg, 100 mg/kg, or 200 mg/kg) was administered 1 h after the adenine and EH treatments. Results TGPF improved weight loss and decreased serum UA, XOD, MCP-1, TNF-α, Cr, and BUN in the rats with hyperuricaemic nephropathy. TGPF downregulated renal URAT1 and GLUT9, upregulated renal OAT1, and ameliorated histopathological changes in the thymus, spleen, and kidney. Conclusion TGPF is promising as a therapeutic agent against hyperuricaemia. It regulates the uric acid transporters and diminished serum uric acid levels, and alleviates renal pathology associated with hyperuricaemia.

According to the characteristics of gout's clinical symptoms, the common gout animal models were analyzed, and the anastomosis and application prospect of the existing gout animal models with the clinical symptoms were discussed. At present, there are three models of gout: hyperuricemia model, gouty arthritis model and gouty nephropathy model. There are many methods of gout modeling, but there is a lack of animal model replication that could reflect the characteristics of gout's clinical symptoms and reflect the etiology of gout, and there is still a gap between the clinical symptoms and the clinical symptoms. In this paper, the characteristics and modeling methods of the three animal models were summarized, and compared with the clinical symptoms and standards, the anastomosis between the existing animal models and the clinical symptoms were analyzed and discussed, and the evaluation and improvement methods of the corresponding animal models were put forward. It provides a reliable experimental method for the treatment of gout by traditional Chinese medicine.

Anosovite (Mg0.3Ti2.7O5) phase was successfully separated from synthesized titanium bearing blast furnace slag by super gravity. Supposing that the titanium exists in the slag in terms of TiO2, the mass fraction of TiO2 is 23.49% in the parallel sample without centrifugal separation. With the parameter of t=5min, T=1553K and the gravity coefficient ranged from 600 to 1000, the mass fraction of TiO2 in the concentrate increase from 40.17% to 58.35%, while the recovery ratio of Ti in the concentrate slightly decrease from 83.49% to 81.81%.

Leaf color mutants commonly found in rice have important implications in basic research and breeding science. In this study, we isolated a pale-green leaf mutant (pgl10) from the offspring of the rice cultivar Nipponbare (Oryza sativa L. spp. japonica) through ethyl methanesulfonate mutagenesis. Compared with the wild-type Nipponbare, the pgl10 mutant had phenotypically pale-green leaves with significantly decreased chlorophyll (a and b) and carotenoid contents. Transmission electron micrographs showed that pgl10 had less grana lamellae of chloroplasts than Nipponbare. The results of tissue-specific gene expression analysis revealed that pgl10 was expressed in various rice organs, including roots, stems, leaves, sheaths, and spikes. The expression of Chl synthesis-associated gene in pgl10 was decreased. Genetic analysis suggested that PGL10 was controlled by a recessive gene. Map-based cloning and genome sequencing data showed that pgl10 was a frameshift mutation caused by a single base insertion on chromosome 10. Bioinformation analysis indicated that PGL10 encoded protochlorophyllide oxidoreductase B. Therefore, pgl10 can be a genetic material for further studies on PGL10.