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147 results on '"Hufnagl, Karin"'

1. Ameliorating Atopy by Compensating Micronutritional Deficiencies in Immune Cells: A Double-Blind Placebo-Controlled Pilot Study

Author

Bartosik, Tina, Jensen, Sebastian A., Afify, Sheriene M., Bianchini, Rodolfo, Hufnagl, Karin, Hofstetter, Gerlinde, Berger, Markus, Bastl, Maximilian, Berger, Uwe, Rivelles, Elisa, Schmetterer, Klaus, Eckl-Dorna, Julia, Brkic, Faris F., Vyskocil, Erich, Guethoff, Sonja, Graessel, Anke, Kramer, Matthias F., Jensen-Jarolim, Erika, and Roth-Walter, Franziska

Published
2022
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2. Ultra‐processed foods, allergy outcomes and underlying mechanisms in children: An EAACI task force report.

Author

Berni Canani, Roberto, Carucci, Laura, Coppola, Serena, D'Auria, Enza, O'Mahony, Liam, Roth‐Walter, Franziska, Vassilopolou, Emilia, Agostoni, Carlo, Agache, Ioana, Akdis, Cezmi, De Giovanni Di Santa Severina, Fiorenza, Faketea, Gavriela, Greenhawt, Matt, Hoffman, Karin, Hufnagl, Karin, Meyer, Rosan, Milani, Gregorio Paolo, Nowak‐Wegrzyn, Anna, Nwaru, Bright, and Padua, Ines

Subjects
FOOD allergy, ADVANCED glycation end-products, ALLERGIC rhinitis, FOOD additives, ATOPIC dermatitis
Abstract

Background: Consumption of ultra‐processed foods [UPFs] may be associated with negative health outcomes. Limited data exist regarding the potential role of UPFs in the occurrence of allergic diseases. The underlying mechanisms underpinning any such associations are also poorly elucidated. Methods: We performed a systematic review and narrative evidence synthesis of the available literature to assess associations between UPF consumption and pediatric allergy outcomes (n = 26 papers), including data on the association seen with the gut microbiome (n = 16 papers) or immune system (n = 3 papers) structure and function following PRISMA guidelines. Results: Dietary exposure to fructose, carbonated soft drinks, and sugar intake was associated with an increased risk of asthma, allergic rhinitis, and food allergies in children. Commercial baby food intake was associated with childhood food allergy. Childhood intake of fructose, fruit juices, sugar‐sweetened beverages, high carbohydrate UPFs, monosodium glutamate, UPFs, and advanced glycated end‐products (AGEs) was associated with the occurrence of allergic diseases. Exposure to UPFs and common ingredients in UPFs seem to be associated with increased occurrence of allergic diseases such as asthma, wheezing, food allergies, atopic dermatitis, and allergic rhinitis, in many, but not all studies. Conclusion: More preclinical and clinical studies are required to better define the link between UPF consumption and the risk of allergies and asthma. These observational studies ideally require supporting data with clearly defined UPF consumption, validated dietary measures, and mechanistic assessments to definitively link UPFs with the risk of allergies and asthma. [ABSTRACT FROM AUTHOR]

Published
2024
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3. Mould allergen Alt a 1 spiked with the micronutrient retinoic acid reduces Th2 response and ameliorates Alternaria allergy in BALB/c mice.

Author

Fakhimahmadi, Aila, Roth‐Walter, Franziska, Hofstetter, Gerlinde, Wiederstein, Markus, Jensen, Sebastian A., Berger, Markus, Szepannek, Nathalie, Bianchini, Rodolfo, Pali‐Schöll, Isabella, Jensen‐Jarolim, Erika, and Hufnagl, Karin

Subjects
ALTERNARIA alternata, MOLECULAR docking, ALTERNARIA, ALLERGENS, EPITOPES, TRETINOIN
Abstract

Background: We investigated the biological function of the mould allergen Alt a 1 as a carrier of micronutrients, such as the vitamin A metabolite retinoic acid (RA) and the influence of RA binding on its allergenicity in vitro and in vivo. Methods: Alt a 1‐RA complex formation was analyzed in silico and in vitro. PBMCs from Alternaria‐allergic donors were stimulated with Alt a 1 complexed with RA (holo‐Alt a 1) or empty apo‐Alt a 1 and analyzed for cytokine production and CD marker expression. Serum IgE‐binding and crosslinking assays to apo‐ and holo‐protein were correlated to B‐cell epitope analysis. Female BALB/c mice already sensitized to Alt a 1 were intranasally treated with apo‐Alt a 1, holo‐Alt a 1 or RA alone before measuring anaphylactic response, serum antibody levels, splenic cytokines and CD marker expression. Results: In silico docking calculations and in vitro assays showed that the extent of RA binding depended on the higher quaternary state of Alt a 1. Holo‐Alt a 1 loaded with RA reduced IL‐13 released from PBMCs and CD3+CD4+CRTh2 cells. Complexing Alt a 1 to RA masked its IgE B‐cell epitopes and reduced its IgE‐binding capacity. In a therapeutic mouse model of Alternaria allergy nasal application of holo‐Alt a 1, but not of apo‐Alt a 1, significantly impeded the anaphylactic response, impaired splenic antigen‐presenting cells and induced IL‐10 production. Conclusion: Holo‐Alt a 1 binding to RA was able to alleviate Th2 immunity in vitro, modulate an ongoing Th2 response and prevent anaphylactic symptoms in vivo, presenting a novel option for improving allergen‐specific immunotherapy in Alternaria allergy. [ABSTRACT FROM AUTHOR]

Published
2024
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4. The protective farm effect against allergies in a holoBLG based FSMP (food for specific medical purposes)-lozenge confers immune resilience in BALB/c mice

Author

Afify, Sheriene, primary, Regner, Andreas, additional, Pacios, Luis, additional, Blokhuis, Bart, additional, jensen, sebastian, additional, Redegeld, Frank, additional, Pali-Schöll, Isabella, additional, Hufnagl, Karin, additional, Bianchini, Rodolfo, additional, Guethoff, Sonja, additional, Kramer, Matthias, additional, Fiocchi, Alessandro, additional, Dvorak, Zdenek, additional, Jensen-Jarolim, Erika, additional, and Roth-Walter, Franziska, additional

Published
2024
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8. Binding to Iron Quercetin Complexes Increases the Antioxidant Capacity of the Major Birch Pollen Allergen Bet v 1 and Reduces Its Allergenicity

Author

Regner, Andreas, Szepannek, Nathalie, Wiederstein, Markus, Fakhimahmadi, Aila, Paciosis, Luis F., Blokhuis, Bart R., Redegeld, Frank A., Hofstetter, Gerlinde, Dvorak, Zdenek, Jensen-Jarolim, Erika, Hufnagl, Karin, Roth-Walter, Franziska, Afd Pharmacology, Afd Pharmacology, Biomedical International R+D GmbH, Bencard Allergie GmbH, Danube Allergy Research Cluster-DARC, Karl-Landsteiner University, Wiederstein, Markus, Fakhimahmadi, Aila, Paciosis, Luis F., Blokhuis, Bart R., Redegeld, Frank A., Hofstetter, Gerlinde, Dvorak, Zdenek, Jensen-Jarolim, Erika, Hufnagl, Karin, and Roth-Walter, Franziska

Subjects
immune resilience, Salicylic-Acid, Receptor, Activation, Protein, Deficiency, Ige, Inhibition, Flavonoids, Mechanism, Immunity, Physiology, holo-Bet v 1, major allergen, immunomodulation, iron, quercetin, birch pollen, pathogenesis-related proteins, innate defense, nutritional immunity, Clinical Biochemistry, Cell Biology, Biochemistry, Molecular Biology, Food Science
Abstract

16 Pág., Bet v 1 is the major allergen in birch pollen to which up to 95% of patients sensitized to birch respond. As a member of the pathogenesis-related PR 10 family, its natural function is implicated in plant defense, with a member of the PR10 family being reported to be upregulated under iron deficiency. As such, we assessed the function of Bet v 1 to sequester iron and its immunomodulatory properties on human immune cells. Binding of Bet v 1 to iron quercetin complexes FeQ2 was determined in docking calculations and by spectroscopy. Serum IgE-binding to Bet v 1 with (holoBet v1) and without ligands (apoBet v 1) were assessed by ELISA, blocking experiments and Western Blot. Crosslinking-capacity of apo/holoBet v 1 were assessed on human mast cells and Arylhydrocarbon receptor (AhR) activation with the human reporter cellline AZ-AHR. Human PBMCs were stimulated and assessed for labile iron and phenotypic changes by flow cytometry. Bet v 1 bound to FeQ2 strongly with calculated Kd values of 1 nm surpassing affinities to quercetin alone nearly by a factor of 1000. Binding to FeQ2 masked IgE epitopes and decreased IgE binding up to 80% and impaired degranulation of sensitized human mast cells. Bet v 1 facilitated the shuttling of quercetin, which activated the anti-inflammatory AhR pathway and increased the labile iron pool of human monocytic cells. The increase of labile iron was associated with an anti-inflammatory phenotype in CD14+monocytes and downregulation of HLADR. To summarize, we reveal for the first time that FeQ2 binding reduces the allergenicity of Bet v 1 due to ligand masking, but also actively contributes anti-inflammatory stimuli to human monocytes, thereby fostering tolerance. Nourishing immune cells with complex iron may thus represent a promising antigen-independent immunotherapeutic approach to improve efficacy in allergen immunotherapy., The study was partly supported by Biomedical Int. R+D GmbH, Vienna, Austria and by Bencard Allergy GmbH, Munich, Germany. A.F. and K.H. were supported by the Danube Allergy Research Cluster-DARC #08 of the Karl-Landsteiner University, Krems, Austria, to E.J.-J.

Published
2023

9. Binding to Iron Quercetin Complexes Increases the Antioxidant Capacity of the Major Birch Pollen Allergen Bet v 1 and Reduces Its Allergenicity

Author

Biomedical International R+D GmbH, Bencard Allergie GmbH, Danube Allergy Research Cluster, Karl Landsteiner University of Health Sciences, Wiederstein, Markus [0000-0002-4750-4746], Fakhimahmadi, Aila [0000-0002-6804-6238], Pacios, Luis F. [0000-0002-0585-4289], Blokhuis, Bart R. [0000-0002-9366-6404], Redegeld, Frank A. [0000-0001-8830-7960], Hofstetter, Gerlinde [0000-0003-2077-4479], Dvorak, Zdenek [0000-0002-3938-3585], Jensen-Jarolim, Erika [0000-0003-4019-5765], Hufnagl, Karin [0000-0002-2288-2468], Roth-Walter, Franziska [0000-0001-5005-9228], Regner, Andreas, Szepannek, Nathalie, Wiederstein, Markus, Fakhimahmadi, Aila, Pacios, Luis F., Blokhuis, Bart R., Redegeld, Frank A., Hofstetter, Gerlinde, Dvorak, Zdenek, Jensen-Jarolim, Erika, Hufnagl, Karin, Roth-Walter, Franziska, Biomedical International R+D GmbH, Bencard Allergie GmbH, Danube Allergy Research Cluster, Karl Landsteiner University of Health Sciences, Wiederstein, Markus [0000-0002-4750-4746], Fakhimahmadi, Aila [0000-0002-6804-6238], Pacios, Luis F. [0000-0002-0585-4289], Blokhuis, Bart R. [0000-0002-9366-6404], Redegeld, Frank A. [0000-0001-8830-7960], Hofstetter, Gerlinde [0000-0003-2077-4479], Dvorak, Zdenek [0000-0002-3938-3585], Jensen-Jarolim, Erika [0000-0003-4019-5765], Hufnagl, Karin [0000-0002-2288-2468], Roth-Walter, Franziska [0000-0001-5005-9228], Regner, Andreas, Szepannek, Nathalie, Wiederstein, Markus, Fakhimahmadi, Aila, Pacios, Luis F., Blokhuis, Bart R., Redegeld, Frank A., Hofstetter, Gerlinde, Dvorak, Zdenek, Jensen-Jarolim, Erika, Hufnagl, Karin, and Roth-Walter, Franziska

Abstract

Bet v 1 is the major allergen in birch pollen to which up to 95% of patients sensitized to birch respond. As a member of the pathogenesis-related PR 10 family, its natural function is implicated in plant defense, with a member of the PR10 family being reported to be upregulated under iron deficiency. As such, we assessed the function of Bet v 1 to sequester iron and its immunomodulatory properties on human immune cells. Binding of Bet v 1 to iron quercetin complexes FeQ2 was determined in docking calculations and by spectroscopy. Serum IgE-binding to Bet v 1 with (holoBet v1) and without ligands (apoBet v 1) were assessed by ELISA, blocking experiments and Western Blot. Crosslinking-capacity of apo/holoBet v 1 were assessed on human mast cells and Arylhydrocarbon receptor (AhR) activation with the human reporter cellline AZ-AHR. Human PBMCs were stimulated and assessed for labile iron and phenotypic changes by flow cytometry. Bet v 1 bound to FeQ2 strongly with calculated Kd values of 1 nm surpassing affinities to quercetin alone nearly by a factor of 1000. Binding to FeQ2 masked IgE epitopes and decreased IgE binding up to 80% and impaired degranulation of sensitized human mast cells. Bet v 1 facilitated the shuttling of quercetin, which activated the anti-inflammatory AhR pathway and increased the labile iron pool of human monocytic cells. The increase of labile iron was associated with an anti-inflammatory phenotype in CD14+monocytes and downregulation of HLADR. To summarize, we reveal for the first time that FeQ2 binding reduces the allergenicity of Bet v 1 due to ligand masking, but also actively contributes anti-inflammatory stimuli to human monocytes, thereby fostering tolerance. Nourishing immune cells with complex iron may thus represent a promising antigen-independent immunotherapeutic approach to improve efficacy in allergen immunotherapy.

Published
2022

10. Secretory protein beta-lactoglobulin in cattle stable dust may contribute to the allergy-protective farm effect

Author

Austrian Science Fund, Biomedical International R+D GmbH, Bencard Allergie GmbH, Pali-Schöll, Isabella [0000-0003-2089-6011], Bianchini, Rodolfo [0000-0003-0351-6937], Afify, Sheriene Moussa [0000-0003-2082-9038], Hofstetter, Gerlinde [0000-0003-2077-4479], Mayerhofer, Hanna [0000-0001-7973-2092], Hufnagl, Karin [0000-0002-2288-2468], Korath, Anna D J [0000-0002-6408-4157], Pranger, Christina [0000-0002-4232-5730], Widhalm, Raimund [0000-0002-8257-9903], Hann, Stephan [0000-0001-5045-7293], Wittek, Thomas [0000-0001-6113-8458], Kasper-Giebl, Anne [0000-0003-2736-2880], Pacios, Luis F [0000-0002-0585-4289], Roth-Walter, Franziska [0000-0001-5005-9228], Vercelli, Donata [0000-0001-5276-8976], von Mutius, Erika [0000-0002-8893-4515], Jensen-Jarolim, Erika [0000-0003-4019-5765], Pali-Schöll, Isabella, Bianchini, Rodolfo, Afify, Sheriene Moussa, Hofstetter, Gerlinde, Winkler, Simona, Ahlers, Stella, Altemeier, Theresa, Mayerhofer, Hanna, Hufnagl, Karin, Korath, Anna D J, Pranger, Christina, Widhalm, Raimund, Hann, Stephan, Wittek, Thomas, Kasper-Giebl, Anne, Pacios, Luis F., Roth-Walter, Franziska, Vercelli, Donata, von Mutius, Erika, Jensen-Jarolim, Erika, Austrian Science Fund, Biomedical International R+D GmbH, Bencard Allergie GmbH, Pali-Schöll, Isabella [0000-0003-2089-6011], Bianchini, Rodolfo [0000-0003-0351-6937], Afify, Sheriene Moussa [0000-0003-2082-9038], Hofstetter, Gerlinde [0000-0003-2077-4479], Mayerhofer, Hanna [0000-0001-7973-2092], Hufnagl, Karin [0000-0002-2288-2468], Korath, Anna D J [0000-0002-6408-4157], Pranger, Christina [0000-0002-4232-5730], Widhalm, Raimund [0000-0002-8257-9903], Hann, Stephan [0000-0001-5045-7293], Wittek, Thomas [0000-0001-6113-8458], Kasper-Giebl, Anne [0000-0003-2736-2880], Pacios, Luis F [0000-0002-0585-4289], Roth-Walter, Franziska [0000-0001-5005-9228], Vercelli, Donata [0000-0001-5276-8976], von Mutius, Erika [0000-0002-8893-4515], Jensen-Jarolim, Erika [0000-0003-4019-5765], Pali-Schöll, Isabella, Bianchini, Rodolfo, Afify, Sheriene Moussa, Hofstetter, Gerlinde, Winkler, Simona, Ahlers, Stella, Altemeier, Theresa, Mayerhofer, Hanna, Hufnagl, Karin, Korath, Anna D J, Pranger, Christina, Widhalm, Raimund, Hann, Stephan, Wittek, Thomas, Kasper-Giebl, Anne, Pacios, Luis F., Roth-Walter, Franziska, Vercelli, Donata, von Mutius, Erika, and Jensen-Jarolim, Erika

Abstract

Growing up on a cattle farm and consuming raw cow's milk protects against asthma and allergies. We expect a cattle-specific protein as active component in this farm effect.

Published
2022

12. Binding to Iron Quercetin Complexes Increases the Antioxidant Capacity of the Major Birch Pollen Allergen Bet v 1 and Reduces Its Allergenicity

Author

Afd Pharmacology, Regner, Andreas, Szepannek, Nathalie, Wiederstein, Markus, Fakhimahmadi, Aila, Paciosis, Luis F., Blokhuis, Bart R., Redegeld, Frank A., Hofstetter, Gerlinde, Dvorak, Zdenek, Jensen-Jarolim, Erika, Hufnagl, Karin, Roth-Walter, Franziska, Afd Pharmacology, Regner, Andreas, Szepannek, Nathalie, Wiederstein, Markus, Fakhimahmadi, Aila, Paciosis, Luis F., Blokhuis, Bart R., Redegeld, Frank A., Hofstetter, Gerlinde, Dvorak, Zdenek, Jensen-Jarolim, Erika, Hufnagl, Karin, and Roth-Walter, Franziska

Published
2023

13. Secretory protein beta-lactoglobulin in cattle stable dust may contribute to the allergy-protective farm effect

Author

Pali-Schöll, Isabella, Bianchini, Rodolfo, Afify, Sheriene Moussa, Hofstetter, Gerlinde, Winkler, Simona, Ahlers, Stella, Altemeier, Theresa, Mayerhofer, Hanna, Hufnagl, Karin, Korath, Anna D J, Pranger, Christina, Widhalm, Raimund, Hann, Stephan, Wittek, Thomas, Kasper-Giebl, Anne, Pacios, Luis F, Roth-Walter, Franziska, Vercelli, Donata, von Mutius, Erika, Jensen-Jarolim, Erika, Austrian Science Fund, Biomedical International R+D GmbH, Bencard Allergie GmbH, Pali-Schöll, Isabella [0000-0003-2089-6011], Bianchini, Rodolfo [0000-0003-0351-6937], Afify, Sheriene Moussa [0000-0003-2082-9038], Hofstetter, Gerlinde [0000-0003-2077-4479], Mayerhofer, Hanna [0000-0001-7973-2092], Hufnagl, Karin [0000-0002-2288-2468], Korath, Anna D J [0000-0002-6408-4157], Pranger, Christina [0000-0002-4232-5730], Widhalm, Raimund [0000-0002-8257-9903], Hann, Stephan [0000-0001-5045-7293], Wittek, Thomas [0000-0001-6113-8458], Kasper-Giebl, Anne [0000-0003-2736-2880], Pacios, Luis F [0000-0002-0585-4289], Roth-Walter, Franziska [0000-0001-5005-9228], Vercelli, Donata [0000-0001-5276-8976], von Mutius, Erika [0000-0002-8893-4515], Jensen-Jarolim, Erika [0000-0003-4019-5765], Pali-Schöll, Isabella, Bianchini, Rodolfo, Afify, Sheriene Moussa, Hofstetter, Gerlinde, Mayerhofer, Hanna, Hufnagl, Karin, Korath, Anna D J, Pranger, Christina, Widhalm, Raimund, Hann, Stephan, Wittek, Thomas, Kasper-Giebl, Anne, Pacios, Luis F, Roth-Walter, Franziska, Vercelli, Donata, von Mutius, Erika, and Jensen-Jarolim, Erika

Subjects
BLG, Immunomodulation, Zinc, Farm effect, Cattle, Stable
Abstract

15 Pág., Growing up on a cattle farm and consuming raw cow's milk protects against asthma and allergies. We expect a cattle-specific protein as active component in this farm effect., This study was supported by the Austrian Science Fund FWF (SFB F4606-B28 and MCCA W1248-B30 to EJJ), in part by Biomedical International R+D GmbH, Vienna, Austria, and by Bencard Allergie GmbH, Munich, Germany.

Published
2022

14. Nutritional Provision of Iron Complexes by the Major Allergen Alt a 1 to Human Immune Cells Decreases Its Presentation.

Author

Fakhimahmadi, Aila, Hasanaj, Ilir, Hofstetter, Gerlinde, Pogner, Clara, Gorfer, Markus, Wiederstein, Markus, Szepannek, Nathalie, Bianchini, Rodolfo, Dvorak, Zdenek, Jensen, Sebastian A., Berger, Markus, Jensen-Jarolim, Erika, Hufnagl, Karin, and Roth-Walter, Franziska

Subjects
QUATERNARY structure, ALLERGENS, ALTERNARIA alternata, IRON, PROTEIN binding, ALANINE aminotransferase, CASEINS
Abstract

Alternaria alternata is a common fungus strongly related with severe allergic asthma, with 80% of affected individuals being sensitized solely to its major allergen Alt a 1. Here, we assessed the function of Alt a 1 as an innate defense protein binding to micronutrients, such as iron–quercetin complexes (FeQ2), and its impact on antigen presentation in vitro. Binding of Alt a 1 to FeQ2 was determined in docking calculations. Recombinant Alt a 1 was generated, and binding ability, as well as secondary and quaternary structure, assessed by UV-VIS, CD, and DLS spectroscopy. Proteolytic functions were determined by casein and gelatine zymography. Uptake of empty apo– or ligand-filled holoAlt a 1 were assessed in human monocytic THP1 cells under the presence of dynamin and clathrin-inhibitors, activation of the Arylhydrocarbon receptor (AhR) using the human reporter cellline AZ-AHR. Human PBMCs were stimulated and assessed for phenotypic changes in monocytes by flow cytometry. Alt a 1 bound strongly to FeQ2 as a tetramer with calculated Kd values reaching pico-molar levels and surpassing affinities to quercetin alone by a factor of 5000 for the tetramer. apoAlt a 1 but not holoAlta 1 showed low enzymatic activity against casein as a hexamer and gelatin as a trimer. Uptake of apo– and holo–Alt a 1 occurred partly clathrin-dependent, with apoAlt a 1 decreasing labile iron in THP1 cells and holoAlt a 1 facilitating quercetin-dependent AhR activation. In human PBMCs uptake of holoAlt a 1 but not apoAlt a 1 significantly decreased the surface expression of the costimulatory CD86, but also of HLADR, thereby reducing effective antigen presentation. We show here for the first time that the presence of nutritional iron complexes, such as FeQ2, significantly alters the function of Alt a 1 and dampens the human immune response, thereby supporting the notion that Alt a 1 only becomes immunogenic under nutritional deprivation. [ABSTRACT FROM AUTHOR]

Published
2023
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16. Binding to Iron Quercetin Complexes Increases the Antioxidant Capacity of the Major Birch Pollen Allergen Bet v 1 and Reduces Its Allergenicity

Author

Regner, Andreas, primary, Szepannek, Nathalie, additional, Wiederstein, Markus, additional, Fakhimahmadi, Aila, additional, Paciosis, Luis F., additional, Blokhuis, Bart R., additional, Redegeld, Frank A., additional, Hofstetter, Gerlinde, additional, Dvorak, Zdenek, additional, Jensen-Jarolim, Erika, additional, Hufnagl, Karin, additional, and Roth-Walter, Franziska, additional

Published
2022
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17. Micronutritional supplementation with a holoBLG-based FSMP (food for special medical purposes)-lozenge alleviates allergic symptoms in BALB/c mice: Imitating the protective farm effect

Author

Biomedical International R+D GmbH, Austrian Science Fund, Bencard Allergie GmbH, Afify, Sheriene Moussa [0000-0003-2082-9038], Pacios, Luis F [0000-0002-0585-4289], Redegeld, Frank A. [0000-0001-8830-7960], Pali-Schöll, Isabella [0000-0003-2089-6011], Hufnagl, Karin [0000-0002-2288-2468], Bianchini, Rodolfo [0000-0003-0351-6937], Guethoff, Sonja [0000-0001-5846-1035], Kramer, Matthias F [0000-0002-3740-4733], Fiocchi, Alessandro [0000-0002-2549-0523], Dvorak, Zdenek [0000-0002-3938-3585], Jensen-Jarolim, Erika [0000-0003-4019-5765], Roth-Walter, Franziska [0000-0001-5005-9228], Afify, Sheriene Moussa, Regner, Andreas, Pacios, Luis F., Blokhuis, Bart R., Jensen, Sebastian A, Redegeld, Frank A., Pali-Schöll, Isabella, Hufnagl, Karin, Bianchini, Rodolfo, Guethoff, Sonja, Kramer, Matthias F, Fiocchi, Alessandro, Dvorak, Zdenek, Jensen-Jarolim, Erika, Roth-Walter, Franziska, Biomedical International R+D GmbH, Austrian Science Fund, Bencard Allergie GmbH, Afify, Sheriene Moussa [0000-0003-2082-9038], Pacios, Luis F [0000-0002-0585-4289], Redegeld, Frank A. [0000-0001-8830-7960], Pali-Schöll, Isabella [0000-0003-2089-6011], Hufnagl, Karin [0000-0002-2288-2468], Bianchini, Rodolfo [0000-0003-0351-6937], Guethoff, Sonja [0000-0001-5846-1035], Kramer, Matthias F [0000-0002-3740-4733], Fiocchi, Alessandro [0000-0002-2549-0523], Dvorak, Zdenek [0000-0002-3938-3585], Jensen-Jarolim, Erika [0000-0003-4019-5765], Roth-Walter, Franziska [0000-0001-5005-9228], Afify, Sheriene Moussa, Regner, Andreas, Pacios, Luis F., Blokhuis, Bart R., Jensen, Sebastian A, Redegeld, Frank A., Pali-Schöll, Isabella, Hufnagl, Karin, Bianchini, Rodolfo, Guethoff, Sonja, Kramer, Matthias F, Fiocchi, Alessandro, Dvorak, Zdenek, Jensen-Jarolim, Erika, and Roth-Walter, Franziska

Abstract

Previously, the protective farm effect was imitated using the whey protein beta-lactoglobulin (BLG) that is spiked with iron-flavonoid complexes. Here, we formulated for clinical translation a lozenge as food for special medical purposes (FSMP) using catechin-iron complexes as ligands for BLG. The lozenge was tested in vitro and in a therapeutical BALB/c mice model.

Published
2021

18. Retinoic acid-loading of the major birch pollen allergen Bet v 1 may improve specific allergen immunotherapy: In silico, in vitro and in vivo data in BALB/c mice

Author

Austrian Science Fund, Hufnagl, Karin [0000-0002-2288-2468], Afify, Sheriene M. [0000-0003-2082-9038], Wallner, Michael [0000-0001-6568-7892], Hauser, Michael [0000-0001-6558-755X], Wiederstein, Markus [0000-0002-4750-4746], Gadermaier, Gabriele [0000-0002-4886-417X], Wildner, Sabrina [0000-0003-2348-7598], Redegeld, Frank A. [0000-0001-8830-7960], Blokhuis, Bart R. [0000-0002-9366-6404], Hofstetter, Gerlinde [0000-0003-2077-4479], Pali-Schöll, Isabella [0000-0003-2089-6011], Roth-Walter, Franziska [0000-0001-5005-9228], Pacios, Luis F. [0000-0002-0585-4289], Jensen-Jarolim, Erika [0000-0003-4019-5765], Hufnagl, Karin, Afify, Sheriene M., Braun, Nina, Wagner, Stefanie, Wallner, Michael, Hauser, Michael, Wiederstein, Markus, Gadermaier, Gabriele, Wildner, Sabrina, Redegeld, Frank A., Blokhuis, Bart R., Hofstetter, Gerlinde, Pali-Schöll, Isabella, Roth-Walter, Franziska, Pacios, Luis F., Jensen-Jarolim, Erika, Austrian Science Fund, Hufnagl, Karin [0000-0002-2288-2468], Afify, Sheriene M. [0000-0003-2082-9038], Wallner, Michael [0000-0001-6568-7892], Hauser, Michael [0000-0001-6558-755X], Wiederstein, Markus [0000-0002-4750-4746], Gadermaier, Gabriele [0000-0002-4886-417X], Wildner, Sabrina [0000-0003-2348-7598], Redegeld, Frank A. [0000-0001-8830-7960], Blokhuis, Bart R. [0000-0002-9366-6404], Hofstetter, Gerlinde [0000-0003-2077-4479], Pali-Schöll, Isabella [0000-0003-2089-6011], Roth-Walter, Franziska [0000-0001-5005-9228], Pacios, Luis F. [0000-0002-0585-4289], Jensen-Jarolim, Erika [0000-0003-4019-5765], Hufnagl, Karin, Afify, Sheriene M., Braun, Nina, Wagner, Stefanie, Wallner, Michael, Hauser, Michael, Wiederstein, Markus, Gadermaier, Gabriele, Wildner, Sabrina, Redegeld, Frank A., Blokhuis, Bart R., Hofstetter, Gerlinde, Pali-Schöll, Isabella, Roth-Walter, Franziska, Pacios, Luis F., and Jensen-Jarolim, Erika

Published
2020

19. Micronutritional supplementation with a holoBLG-based FSMP (food for special medical purposes)-lozenge alleviates allergic symptoms in BALB/c mice: Imitating the protective farm effect

Author

Afd Pharmacology, Pharmacology, Afify, Sheriene Moussa, Regner, Andreas, Pacios, Luis F, Blokhuis, Bart R, Jensen, Sebastian A, Redegeld, Frank A, Pali-Schöll, Isabella, Hufnagl, Karin, Bianchini, Rodolfo, Guethoff, Sonja, Kramer, Matthias F, Fiocchi, Alessandro, Dvorak, Zdenek, Jensen-Jarolim, Erika, Roth-Walter, Franziska, Afd Pharmacology, Pharmacology, Afify, Sheriene Moussa, Regner, Andreas, Pacios, Luis F, Blokhuis, Bart R, Jensen, Sebastian A, Redegeld, Frank A, Pali-Schöll, Isabella, Hufnagl, Karin, Bianchini, Rodolfo, Guethoff, Sonja, Kramer, Matthias F, Fiocchi, Alessandro, Dvorak, Zdenek, Jensen-Jarolim, Erika, and Roth-Walter, Franziska

Published
2022

20. Secretory protein beta‐lactoglobulin in cattle stable dust may contribute to the allergy‐protective farm effect

Author

Pali‐Schöll, Isabella, primary, Bianchini, Rodolfo, additional, Afify, Sheriene Moussa, additional, Hofstetter, Gerlinde, additional, Winkler, Simona, additional, Ahlers, Stella, additional, Altemeier, Theresa, additional, Mayerhofer, Hanna, additional, Hufnagl, Karin, additional, Korath, Anna D. J., additional, Pranger, Christina, additional, Widhalm, Raimund, additional, Hann, Stephan, additional, Wittek, Thomas, additional, Kasper‐Giebl, Anne, additional, Pacios, Luis F., additional, Roth‐Walter, Franziska, additional, Vercelli, Donata, additional, von Mutius, Erika, additional, and Jensen‐Jarolim, Erika, additional

Published
2022
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21. Micronutritional supplementation with a holoBLG‐based FSMP (food for special medical purposes)‐lozenge alleviates allergic symptoms in BALB/c mice: Imitating the protective farm effect

Author

Afify, Sheriene Moussa, primary, Regner, Andreas, additional, Pacios, Luis F., additional, Blokhuis, Bart R., additional, Jensen, Sebastian A., additional, Redegeld, Frank A., additional, Pali‐Schöll, Isabella, additional, Hufnagl, Karin, additional, Bianchini, Rodolfo, additional, Guethoff, Sonja, additional, Kramer, Matthias F., additional, Fiocchi, Alessandro, additional, Dvorak, Zdenek, additional, Jensen‐Jarolim, Erika, additional, and Roth‐Walter, Franziska, additional

Published
2021
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22. Bet v 1 from birch pollen is a hypoallergen with vitamin D3 in the pocket

Author

Hufnagl, Karin, primary, Kromp, Livia, additional, Bianchini, Rodolfo, additional, Afify, Sheriene Moussa, additional, Wiederstein, Markus, additional, Redegeld, Frank A., additional, Zuvalova, Iveta, additional, Dvorak, Zdenek, additional, Hofstetter, Gerlinde, additional, Roth‐Walter, Franziska, additional, Pacios, Luis F., additional, and Jensen‐Jarolim, Erika, additional

Published
2021
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23. Functional iron‐deficiency in women with allergic rhinitis is associated with symptoms after nasal provocation and lack of iron‐sequestering microbes

Author

Petje, Lisa‐Marie, primary, Jensen, Sebastian A., additional, Szikora, Sebastian, additional, Sulzbacher, Martin, additional, Bartosik, Tina, additional, Pjevac, Petra, additional, Hausmann, Bela, additional, Hufnagl, Karin, additional, Untersmayr, Eva, additional, Fischer, Lisa, additional, Vyskocil, Erich, additional, Eckl‐Dorna, Julia, additional, Jensen‐Jarolim, Erika, additional, Hofstetter, Gerlinde, additional, Afify, Sheriene Moussa, additional, Krenn, Claus G., additional, Roth, Georg A., additional, Rivelles, Elisa, additional, Hann, Stephan, additional, and Roth‐Walter, Franziska, additional

Published
2021
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24. Bet v 1 from birch pollen is a hypoallergen with vitamin D3 in the pocket

Author

Afd Pharmacology, Pharmacology, Hufnagl, Karin, Kromp, Livia, Bianchini, Rodolfo, Afify, Sheriene Moussa, Wiederstein, Markus, Redegeld, Frank A, Zuvalova, Iveta, Dvorak, Zdenek, Hofstetter, Gerlinde, Roth-Walter, Franziska, Pacios, Luis F, Jensen-Jarolim, Erika, Afd Pharmacology, Pharmacology, Hufnagl, Karin, Kromp, Livia, Bianchini, Rodolfo, Afify, Sheriene Moussa, Wiederstein, Markus, Redegeld, Frank A, Zuvalova, Iveta, Dvorak, Zdenek, Hofstetter, Gerlinde, Roth-Walter, Franziska, Pacios, Luis F, and Jensen-Jarolim, Erika

Published
2021

26. Filling the Antibody Pipeline in Allergy: PIPE Cloning of IgE, IgG1 and IgG4 against the Major Birch Pollen Allergen Bet v 1

Author

Köhler, Verena K., Crescioli, Silvia, Fazekas-Singer, Judit, Bax, Heather J., Hofer, Gerhard, Pranger, Christina L., Hufnagl, Karin, Bianchini, Rodolfo, Flicker, Sabine, Keller, Walter, Karagiannis, Sophia N., and Jensen-Jarolim, Erika

Subjects
FcεRI, IgG1, in vitro, allergy, lcsh:Chemistry, blocking antibodies, lcsh:Biology (General), lcsh:QD1-999, allergen immunotherapy, antibodies, IgG4, IgE, CD23, lcsh:QH301-705.5
Abstract

Birch pollen allergy is among the most prevalent pollen allergies in Northern and Central Europe. This IgE-mediated disease can be treated with allergen immunotherapy (AIT), which typically gives rise to IgG antibodies inducing tolerance. Although the main mechanisms of allergen immunotherapy (AIT) are known, questions regarding possible Fc-mediated effects of IgG antibodies remain unanswered. This can mainly be attributed to the unavailability of appropriate tools, i.e., well-characterised recombinant antibodies (rAbs). We hereby aimed at providing human rAbs of several classes for mechanistic studies and as possible candidates for passive immunotherapy. We engineered IgE, IgG1, and IgG4 sharing the same variable region against the major birch pollen allergen Bet v 1 using Polymerase Incomplete Primer Extension (PIPE) cloning. We tested IgE functionality and IgG blocking capabilities using appropriate model cell lines. In vitro studies showed IgE engagement with Fc&epsilon, RI and CD23 and Bet v 1-dependent degranulation. Overall, we hereby present fully functional, human IgE, IgG1, and IgG4 sharing the same variable region against Bet v 1 and showcase possible applications in first mechanistic studies. Furthermore, our IgG antibodies might be useful candidates for passive immunotherapy of birch pollen allergy.

Published
2020

27. Retinoic acid-loading of the major birch pollen allergen Bet v 1 may improve specific allergen immunotherapy: in silico, in vitro and in vivo data in BALB/c mice

Author

Afd Pharmacology, Pharmacology, Hufnagl, Karin, Moussa Afify, Sheriene, Braun, Nina, Wagner, Stefanie, Wallner, Michael, Hauser, Michael, Wiederstein, Markus, Gadermaier, Gabriele, Wildner, Sabrina, Redegeld, Frank A, Blokhuis, Bart R, Hofstetter, Gerlinde, Pali-Schöll, Isabella, Roth-Walter, Franziska, Pacios, Luis F, Jensen-Jarolim, Erika, Afd Pharmacology, Pharmacology, Hufnagl, Karin, Moussa Afify, Sheriene, Braun, Nina, Wagner, Stefanie, Wallner, Michael, Hauser, Michael, Wiederstein, Markus, Gadermaier, Gabriele, Wildner, Sabrina, Redegeld, Frank A, Blokhuis, Bart R, Hofstetter, Gerlinde, Pali-Schöll, Isabella, Roth-Walter, Franziska, Pacios, Luis F, and Jensen-Jarolim, Erika

Published
2020

29. Cow’s milk protein β-lactoglobulin confers resilience against allergy by targeting complexed iron into immune cells

Author

Roth-Walter, Franziska, primary, Afify, Sheriene Moussa, additional, Pacios, Luis F., additional, Blokhuis, Bart R., additional, Redegeld, Frank, additional, Regner, Andreas, additional, Petje, Lisa-Marie, additional, Fiocchi, Alessandro, additional, Untersmayr, Eva, additional, Dvorak, Zdenek, additional, Hufnagl, Karin, additional, Pali-Schöll, Isabella, additional, and Jensen-Jarolim, Erika, additional

Published
2021
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30. Micronutritional supplementation with a holoBLG‐based FSMP (food for special medical purposes)‐lozenge alleviates allergic symptoms in BALB/c mice: Imitating the protective farm effect.

Author

Afify, Sheriene Moussa, Regner, Andreas, Pacios, Luis F., Blokhuis, Bart R., Jensen, Sebastian A., Redegeld, Frank A., Pali‐Schöll, Isabella, Hufnagl, Karin, Bianchini, Rodolfo, Guethoff, Sonja, Kramer, Matthias F., Fiocchi, Alessandro, Dvorak, Zdenek, Jensen‐Jarolim, Erika, and Roth‐Walter, Franziska

Subjects
ANTIGEN presenting cells, MAST cells, MILK allergy, LACTOGLOBULINS, ANTIGEN presentation, WHEY proteins
Abstract

Background: Previously, the protective farm effect was imitated using the whey protein beta‐lactoglobulin (BLG) that is spiked with iron‐flavonoid complexes. Here, we formulated for clinical translation a lozenge as food for special medical purposes (FSMP) using catechin‐iron complexes as ligands for BLG. The lozenge was tested in vitro and in a therapeutical BALB/c mice model. Methods: Binding of iron‐catechin into BLG was confirmed by spectroscopy and docking calculations. Serum IgE binding of children allergic or tolerating milk was assessed to loaded (holo‐) versus empty (apo‐) BLG and for human mast cell degranulation. BLG and Bet v 1 double‐sensitized mice were orally treated with the holoBLG or placebo lozenge, and immunologically analysed after systemic allergen challenge. Human PBMCs of pollen allergic subjects were flow cytometrically assessed after stimulation with apoBLG or holoBLG using catechin‐iron complexes as ligands. Results: One major IgE and T cell epitope were masked by catechin‐iron complexes, which impaired IgE binding of milk‐allergic children and degranulation of mast cells. In mice, only supplementation with the holoBLG lozenge reduced clinical reactivity to BLG and Bet v 1, promoted Tregs, and suppressed antigen presentation. In allergic subjects, stimulation of PBMCs with holoBLG led to a significant increase of intracellular iron in circulating CD14+ cells with significantly lower expression of HLADR and CD86 compared to their stimulation with apoBLG. Conclusion: The FSMP lozenge targeted antigen presenting cells and dampened immune activation in human immune cells and allergic mice in an antigen‐non‐specific manner, thereby conferring immune resilience against allergic symptoms. The whey protein beta‐lactoglobulin BLG binding to micronutrients such as iron‐catechin complexes (holoBLG) was recognized less by IgE antibodies of children allergic to milk, leading to reduced mast cell degranulation. BLG also facilitated catechin‐dependent activation of the anti‐inflammatory arylhydrocarbon receptor. In mice, oral application of a holoBLG‐based lozenge which also contained zinc and vitamin A, improved the allergic symptoms of double‐sensitized mice independent of the allergens they were challenged with. Double‐sensitized mice supplemented with the holoBLG lozenge also had reduced levels of allergen‐specific antibodies and a lower frequency of activated antigen presenting cells. [ABSTRACT FROM AUTHOR]

Published
2022
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32. Retinoic acid‐loading of the major birch pollen allergen Bet v 1 may improve specific allergen immunotherapy: In silico, in vitro and in vivo data in BALB/c mice

Author

Hufnagl, Karin, primary, Afify, Sheriene Moussa, additional, Braun, Nina, additional, Wagner, Stefanie, additional, Wallner, Michael, additional, Hauser, Michael, additional, Wiederstein, Markus, additional, Gadermaier, Gabriele, additional, Wildner, Sabrina, additional, Redegeld, Frank A., additional, Blokhuis, Bart R., additional, Hofstetter, Gerlinde, additional, Pali‐Schöll, Isabella, additional, Roth‐Walter, Franziska, additional, Pacios, Luis F., additional, and Jensen‐Jarolim, Erika, additional

Published
2020
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41. Retinoic acid prevents immunogenicity of milk lipocalin Bos d 5 through binding to its immunodominant T-cell epitope

Author

Hufnagl, Karin, primary, Ghosh, Debajyoti, additional, Wagner, Stefanie, additional, Fiocchi, Alessandro, additional, Dahdah, Lamia, additional, Bianchini, Rodolfo, additional, Braun, Nina, additional, Steinborn, Ralf, additional, Hofer, Martin, additional, Blaschitz, Marion, additional, Roth, Georg A., additional, Hofstetter, Gerlinde, additional, Roth-Walter, Franziska, additional, Pacios, Luis F., additional, and Jensen-Jarolim, Erika, additional

Published
2018
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42. IgG4 drives M2a macrophages to a regulatory M2b‐like phenotype: potential implication in immune tolerance.

Author

Bianchini, Rodolfo, Roth‐Walter, Franziska, Ohradanova‐Repic, Anna, Flicker, Sabine, Hufnagl, Karin, Fischer, Michael Bernhard, Stockinger, Hannes, and Jensen‐Jarolim, Erika

Subjects
IMMUNOLOGICAL tolerance, IMMUNE complexes, MACROPHAGES, FLOW cytometry, GRASS pollen, IMMUNOGLOBULIN E
Abstract

Background: Macrophages can be converted in vitro into immunoregulatory M2b macrophages in the presence of immune complexes (ICs), but the role of the specific subclasses IgG1 or IgG4 in this phenotypic and functional change is not known. Objective: We aimed to refine the original method by applying precisely defined ICs of the subclasses IgG4 or IgG1 constructed by two independent methods. Methods: Monocyte‐derived macrophages (MDMs) were treated with M‐CSF, followed by IL‐4/IL‐13 to induce the M2a allergic phenotype. To mimic unspecific or allergen‐specific ICs, plates were coated with myeloma IgG1 or IgG4, or with grass pollen allergen Phl p 5 followed by recombinant human Phl p 5‐specific IgG1 or IgG4. M2a polarized macrophages were then added, cultured, and examined for cellular markers and cytokines by flow cytometry, ELISA, and rtPCR. Alternatively, immune complexes with IgG1 or IgG4 were formed using protein L. Results: IgG4 ICs down regulated CD163 and CD206 on M2a cells, and significantly increased IL‐10, IL‐6, TNFα, and CCL1 secretion, indicating a shift to an M2b‐like phenotype. Treatment with IgG4 ICs resulted in expression of FcγRII and down modulation of FcγRII compared with IgG1 treated cells (P = 0.0335) or untreated cells (P < 0.00001). Conclusion: Immune complexes with subclasses IgG1 and IgG4 can in vitro be generated by plate absorption, and in fluid form by protein L. Cross‐linking of FcγRIIb by the IgG4 subclass redirects pro‐allergic M2a macrophages to an M2b‐like immunosuppressive phenotype. This suggests an interplay of macrophages with IgG4 in immune tolerance, likely relevant in allergen immunotherapy. This study highlights the central role of IgG4 in immune tolerance. The treatment of M2a with ICs consisting of IgG4 shows a considerably greater effect in promoting an M2b‐like phenotype than with IgG1‐containing ICs. The secretion of CCL1 and IL‐10 by M2b‐like macrophages is important to establish a tolerogenic microenvironment. [ABSTRACT FROM AUTHOR]

Published
2019
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43. WAO International Scientific Conference (WISC 2016) Abstracts

Author

Bao, Jun, Wang, Yi-Hui, Liu, Quan-Hua, Bao, Yi-Xiao, Azouz, Nurit, Caldwell, Julie, Ray, Leanne, Rochman, Mark, Mingler, Melissa, Eilerman, Matthew, Wen, Ting, Myers, Jocelyn Biagini, Hershey, Gurjit Khurana, Kottyan, Leah, Martin, Lisa, Marc, Rothenberg, Gonzalez-Uribe, Victor, Del Rio-Chivardi, Jaime, Del Rio-Navarro, Blanca, Lou, Hongfei, Ma, Siyuan, Zhao, Yan, Cao, Feifei, He, Fei, Liu, Zhongyan, Wang, Chengshuo, Bachert, Claus, Zhang, Luo, Abrams, Elissa, Becker, Allan, Kandhare, Amit, Bodhankar, Subhash, Grossman, Nicole, Doros, Gheorghe, Laden, Francine, Fuhlbrigge, Anne, Wechsler, Michael, Pace, Wilson, Yawn, Barbara, Israel, Elliot, Lee, Junehyuk, Adler, Frederick, Kim, Peter, Huang, Yung Feng, Chen, Ying Yao, Pan, Chiun Yen, Lee, Herng Sheng, Khalemsky, Michael, Schwartz, David G., Kolkhir, Pavel, Pogorelov, Dmitry, Kochergin, Nikolay, Komarow, Hirsh, Young, Michael, Eisch, Robin, Scott, Linda, Metcalfe, Dean, Singer, Alexander, Wakeman, Andrew, Gerstner, Thomas, Song, Woo-Jung, Shim, Ji-Su, Won, Ha-Kyeong, Kang, Sung-Yoon, Sohn, Kyoung-Hee, Kim, Byung-Keun, Jo, Eun-Jung, Kim, Min-Hye, Kim, Sang-Heon, Park, Heung-Woo, Kim, Sun-Sin, Chang, Yoon-Seok, Morice, Alyn H., Lee, Byung-Jae, Cho, Sang-Heon, Min, Kyung-Up, Boscolo, Maria Assunta, Brivio, Giulio, Bosisio, Sergio, Manzocchi, Nicoletta, Pulixi, Edoardo, Grignani, Giulia, D’Andrea, Eloisia, Ricci, Massimo, Passini, Elena, Italia, Maurizio, Urrutia-Pereira, Marilyn, Fagundes, Stefani, Oliano, Vinicius Jardim, Solé, Dirceu, Benzaquen, Sadia, Aragaki, Alejandro, Balestra, Ricardo, Harden, Dawn, Caudell-Stamper, Danielle, Glady, Gilbert, Holbreich, Mark, Lyakhovska, Nataliya, Kaidashev, Igor, Bystron, Jaromir, Hutyrova, Beata, Balakirski, Galina, Vanstreels, Luk, Wurpts, Gerda, Merk, Hans F., Baron, Jens Malte, Plange, Johanna, Rihs, Hans-Peter, Raulf, Monika, Roeseler, Stefani, Tolcachier, Alberto, Chamorro, Armando, Otero, Ruth, Brooks, Joel, Hess, Michael, Benz, Jared, MacDonald, Joseph, Chatha, Usma, Lent, Dale, Köse, Şükran, Tatar, Bengü Gireniz, Akkoçlu, Gülgün, Çukurova, İbrahim, Ödemiş, İlker, Toker, Ayşin Kılınç, Hasssan, Abdullahi, Gobir, Abdulrazaq Abdullahi, Kim, Cheol-Woo, Choi, Young Hwa, Lee, Jeong Hye, Cho, Rae Jeong, Nam, Yu Ran, Nam, Joo Hyun, Kim, Woo Kyung, Filipovic, Ivana, Zivkovic, Zorica, Filipovic, Djordje, Shik, Dana, Smith, Andrew, Hsi, Wang Yui, Friedman, Stuart, Gizaw, Yonatan, Bakhda, Rima, Mohammed, Kumail, Wasserman, Richard, Hague, Angela, Pence, Deanna, Rolen, Joanna, Sugerman, Robert, Silvers, Stacy, Kamili, Qurat, Knauer, Nadezhda, Zazernyi, Alexandr, Blinova, Elena, Demina, Daria, Kozlov, Vladimir, Agrawal, Komal, Kale, Sagar, Arora, Naveen, Vasilkova, Volha, Mokhort, Tatiana, Silvers, William, Eisenberg, Rachel, Mehta, Rushita, Rubinstein, Arye, Aston, Antony, Turner, Paul, Ruiz-Garcia, Monica, Boyle, Robert, Brown, Simon, Schejter, Yael Dinur, Ovadia, Adi, Kim, Vy, Reid, Brenda, Roifman, Chaim, Rosenfield, Lana, Avilla, Ernie, Harada, Laurie, Allen, Marilyn, Waserman, Susan, Yoon, Ho Joo, Koo, Gun Woo, Chang, Suk-Il, Yoon, Hye-Ran, Park, Dong Won, Park, Tai Sun, Moon, Ji-Yong, Kim, Tae Hyung, Sohn, Jang Won, Shin, Dong Ho, Jorjoliani, Tsici, Jorjoliani, Lia, Adamia, Nino, katamadze, Nona, Ramachandra, Deepika, Jorjoliani, Liana, Karseladze, Rusudan, Saginadze, Lali, Chkuaseli, Natalia, Dolgova, Anna, Stukolova, Olga, Sudina, Anna, Cherkashina, Anna, Shipulin, German, Rosenthal, Richard, Howe, Harvey, Knause, Paul, Greemberg, Rony, De Bruycker, Jean Jacques, Fernandez, Isabel, Le Deist, Françoise, Haddad, Elie, Rha, Yeong Ho, Lee, Kyung Suk, Choi, Sun Hee, Tam, Herman, Simons, Estelle, Simons, Elinor, Golebiowska-Wawrzyniak, Maria, Markiewicz, Katarzyna, Faitelson, Yoram, Stein, Miguel, Mandelberg, Avigdor, Dalal, Ilan, Levin, Michael, Hobane, Lelani, Basera, Wisdom, Botha, Maresa, Gray, Claudia, Zar, Heather, Kjaeva, Biserka Jovkovska, Arsovski, Zoran, Grivcheva-Panovska, Vesna, Odebode, Adeyinka, Adekunle, Adedotun, Adeonipekun, Peter, Farombi, Ebenezer, Camacho-Ordoñez, Nadezhda, Martinez-Vázquez, Alejandrina Josefina, García-Cruz, María de la Luz H., Tan, Qi, Min, Rui, Dai, Guan-qun, Xie, Wei-Ping, Mao, Huang, Wang, Hong, Yadav, Rakesh, Singh, Sneha, Yadav, Divya, Khaleva, Ekaterina, Bahnson, Henry T., Franz, Amber, Garvey, Lene Heise, Jay, Nicola, Haque, Rubaiyat, Fox, Adam, Lack, Gideon, du Toit, George, Radic, Snezana, Milenkovic, Branislava, Neskovic, Ana, Danojevic, Ljiljana, Nachshon, Liat, Goldberg, Michael, Levy, Michael, Katz, Yitzhak, Elizur, Arnon, Rosario, Cristine, Kasper, Juliana, Chong-Neto, Herbeto, Riedi, Carlos, Rosario, Nelson, Levy, Michael B., Har-Even, Ronly, Carmel, Mor, Goldberg, Michael R., Kherkheulidze, Maia, Kavlashvili, Nani, Kandelaki, Eka, Adamai, Nino, Ubiria, Irma, Burke, Andrea, Kastner, Monika, Zheleva, Denica, Darlenski, Razvigor, Bozinakis, Konstantinos, Kriebardis, Anastasios, Styliara, Sofia, Karastathi, Aikaterini, Farmakas, Nikolaos, Ribeiro, Maria Luiza Kraft Kohler, Barcellos, Ana Carolina, Silva, Hannah Gabriele Ferreira, Carletto, Luís Henrique Mattei, Bet, Marcela Carolina, Rossetto, Nathalia Zorze, Rosario, Nelson Augusto, Chong-Neto, Herberto Jose, Valença, Fernanda, Novaes, Marina, Gomes, Mariana, Seifert, Carla, Neto, Alfredo, Loyola, Flavia, Rios, José, Silva, Tatiana, Neves, Aline, Abadoglu, Oznur, Gemicioglu, Bilun, Bayram, Hasan, Cimrin, Arif, Akyildiz, Levent, Cilli, Aykut, Gunen, Hakan, Ozlu, Tevfik, Suerdem, Mecit, Uzaslan, Esra, Misirligil, Zeynep, Ristic-Stojanovic, Snezana, Milicevic, A., Milenkovic, A., Cvejic, Jelena, Jankovic, Jelena, Dimic-Janjic, Sanja, Djurdjevic, Natasa, Barzylovych, Vladyslava, Umanets, Tetiana, Barzylovych, Anastasia, Winkler, Karyn, Margarinos, Jessica, Martin, Dylan, Nowakowski, Maja, Joks, Rauno, Zangen, Tsili, Bernadsky, Olga, Boaz, Mona, Hermann, Gratiana, Aviv, Rachel, Kuperboim, Olga, Ramichanov, Larisa, Broide, Efrat, Shamir, Raanan, Zevit, Noam, Shaoul, Ron, Fich, Alex, Levine, Arie, Melamed, Isaac, Gangwar, Roopesh Singh, Minai-Fleminger, Yael, Seaf, Mansour, Gutgold, Amichai, Shikotra, Aarti, Chauhan, Anoop, Holgate, Stephen, Bradding, Peter, Howarth, Peter, Eliashar, Ron, Berkman, Neville, Levi-Schaffer, Francesca, Woo, Sung-il, Celik, Betul, Bulut, Tangul, Yalcin, Arzu Didem, Caldas, Luiz Querino, Confino-Cohen, Ronit, Rosman, Yossi, Goldberg, Arnon, Breuer, Oded, Singh, Roopesh, Barhoum, Ahlam, Kerem, Eitan, Slavyanskaya, Tatiana, Sepiashvili, Revaz, Blinova, Elena A., Pashkina, Ekaterina A., Leonova, Marina I., Nepomnyaschikh, Vera M., Demina, Darya V., Kozlov, Vladimir A., Shamri, Revital, Young, Kristen M., Weller, Peter F., de Paula Vieira, Rodolfo, Oliveira-Junior, Manoel Carneiro, Damasceno-Rodrigues, Nilsa Regina, Arantes-Costa, Fernanda Magalhães, Martins, Milton Arruda, Oliveira, Ana Paula Ligeiro, Bernard, Alfred, Sardella, Antonia, Voisin, Catherine, Royce, Simon, Philpott, Hamish, Nandurkar, Sanjay, Thien, Francis, Gibson, Peter, Bianchini, Rodolfo, Roth-Walter, Franziska, Ohradanova-Repic, Anna, Hofstetter, Gerlinde, Herrmann, Ina, Carvalho, Maria Isabel, Hufnagl, Karin, Bajna, Erika, Roth, Georg, Stockinger, Hannes, Jensen-Jarolim, Erika, Almog, Meital, Kessel, Aharon, Apov, Larisa, Salguero, Carlos Sanchez, Chacon, Alvaro Sanchez, Nazarov, Abbos, Ergashev, Shaxbos, Nesterova, Irina, Kovaleva, Svetlana, Chudilova, Galina, Lomtatidze, Ludmila, De Schryver, Sarah, Dery, Alizee, Clarke, Ann, Nadeau, Kari, Weatherall, Kimberly, Greenwood, Celia, Daley, Denise, Asai, Yuka, Ben-Shoshan, Moshe, Balmasova, Irina, Malova, Elena, Wagner, Stefanie, Pacios, Luis F., Wallner, Michael, Wiederstein, Markus, Tevs, Anna E., Tataurshchikova, Nataly, Sangidorj, Baigalmaa, Ronzhina, Anna, Chikhladze, Manana, Wirz, Oliver F., van de Veen, Willem, Mirer, David, Morita, Hideaki, Altunbulakli, Can, Johnston, Sebastian L., Glanville, Nicholas, Papadopoulos, Nikolaos G., Akdis, Cezmi A., Akdis, Mübeccel, Reshef, Avner, Riedl, Marc, Panovska, Vesna Grivcheva, Moldovan, Dumitru, Baker, James, Yang, William H., Andrejevic, Sladjana, Lockey, Richard F., Hakl, Roman, Kivity, Shmuel, Bellizzi, Luca, Harper, Joseph R., Relan, Anurag, and Cicardi, Marco

Subjects
lcsh:Immunologic diseases. Allergy, Pulmonary and Respiratory Medicine, 010504 meteorology & atmospheric sciences, business.industry, Immunology, Library science, Meeting Abstracts, 01 natural sciences, 03 medical and health sciences, 0302 clinical medicine, 030228 respiratory system, Immunology and Allergy, Medicine, lcsh:RC581-607, business, 0105 earth and related environmental sciences, Wechsler Intelligence Scale for Children
Published
2017

46. Prevention of birch pollen-related food allergy by mucosal treatment with multi-allergen-chimers in mice

Author

Hoflehner, Elisabeth, Hufnagl, Karin, Schabussova, Irma, Jasinska, Joanna, Hoffmann-Sommergruber, Karin, Bohle, Barbara, Maizels, Rick M., and Wiedermann, Ursula

Subjects
Proteomics, Anatomy and Physiology, Mouse, lcsh:Medicine, Protein Engineering, T-Lymphocytes, Regulatory, Cell Degranulation, Epitopes, Mice, Immune Physiology, lcsh:Science, Betula, Immunity, Cellular, Mice, Inbred BALB C, Allergy and Hypersensitivity, Vaccination, Animal Models, Recombinant Proteins, Basophils, Cytokines, Medicine, Pollen, Female, Immunotherapy, Food Hypersensitivity, Research Article, Immunology, Peptide Mapping, Immunomodulation, Model Organisms, Vaccine Development, Immune Tolerance, otorhinolaryngologic diseases, Animals, RNA, Messenger, Antibodies, Blocking, Biology, Administration, Intranasal, lcsh:R, Immunity, Immunoregulation, Allergens, Antigens, Plant, Immunoglobulin E, Immunity, Humoral, Nasal Mucosa, Gene Expression Regulation, Clinical Immunology, Immunization, lcsh:Q
Abstract

Background Among birch pollen allergic patients up to 70% develop allergic reactions to Bet v 1-homologue food allergens such as Api g 1 (celery) or Dau c 1 (carrot), termed as birch pollen-related food allergy. In most cases, specific immunotherapy with birch pollen extracts does not reduce allergic symptoms to the homologue food allergens. We therefore genetically engineered a multi-allergen chimer and tested if mucosal treatment with this construct could represent a novel approach for prevention of birch pollen-related food allergy. Methodology BALB/c mice were poly-sensitized with a mixture of Bet v 1, Api g 1 and Dau c 1 followed by a sublingual challenge with carrot, celery and birch pollen extracts. For prevention of allergy sensitization an allergen chimer composed of immunodominant T cell epitopes of Api g 1 and Dau c 1 linked to the whole Bet v 1 allergen, was intranasally applied prior to sensitization. Results Intranasal pretreatment with the allergen chimer led to significantly decreased antigen-specific IgE-dependent β-hexosaminidase release, but enhanced allergen-specific IgG2a and IgA antibodies. Accordingly, IL-4 levels in spleen cell cultures and IL-5 levels in restimulated spleen and cervical lymph node cell cultures were markedly reduced, while IFN-γ levels were increased. Immunomodulation was associated with increased IL-10, TGF-β and Foxp3 mRNA levels in NALT and Foxp3 in oral mucosal tissues. Treatment with anti-TGF-β, anti-IL10R or anti-CD25 antibodies abrogated the suppression of allergic responses induced by the chimer. Conclusion Our results indicate that mucosal application of the allergen chimer led to decreased Th2 immune responses against Bet v 1 and its homologue food allergens Api g 1 and Dau c 1 by regulatory and Th1-biased immune responses. These data suggest that mucosal treatment with a multi-allergen vaccine could be a promising treatment strategy to prevent birch pollen-related food allergy.

Published
2012

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